Neuronal acetylcholine receptor subunit alpha-4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30031159 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA3 (cholinergic receptor nicotinic alpha 3 subunit [Source:HGNC Symbol;Acc:HGNC:1957]) HSP 1 Score: 206.453 bits (524), Expect = 2.356e-58 Identity = 134/401 (33.42%), Postives = 231/401 (57.61%), Query Frame = 1
Query: 199 GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEK--LHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
E +L ++ +DY + I RPV NV D V++ + +++ Q++ +DE NQI+ N+W K W D L WNP ++ E +R+P KIW PDIVLYN A D ++ +K + + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K PD+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + L +E+ N N R + K E + H+ K++ NL+ SSS+
Sbjct: 34 AEHRLFERLFEDYNE---IIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKAL--LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSN-LNCFSRAESKGCKEGYPC-QDGMCGYCHHRRIKISNFSANLTRSSSS 425
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA3 (cholinergic receptor nicotinic alpha 3 subunit [Source:HGNC Symbol;Acc:HGNC:1957]) HSP 1 Score: 206.453 bits (524), Expect = 2.356e-58 Identity = 134/401 (33.42%), Postives = 231/401 (57.61%), Query Frame = 1
Query: 199 GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEK--LHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
E +L ++ +DY + I RPV NV D V++ + +++ Q++ +DE NQI+ N+W K W D L WNP ++ E +R+P KIW PDIVLYN A D ++ +K + + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K PD+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + L +E+ N N R + K E + H+ K++ NL+ SSS+
Sbjct: 34 AEHRLFERLFEDYNE---IIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKAL--LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSN-LNCFSRAESKGCKEGYPC-QDGMCGYCHHRRIKISNFSANLTRSSSS 425
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA3 (cholinergic receptor nicotinic alpha 3 subunit [Source:HGNC Symbol;Acc:HGNC:1957]) HSP 1 Score: 205.682 bits (522), Expect = 3.670e-58 Identity = 134/401 (33.42%), Postives = 231/401 (57.61%), Query Frame = 1
Query: 199 GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEK--LHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
E +L ++ +DY +I RPV NV D V++ + +++ Q++ +DE NQI+ N+W K W D L WNP ++ E +R+P KIW PDIVLYN A D ++ +K + + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K PD+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + L +E+ N N R + K E + H+ K++ NL+ SSS+
Sbjct: 34 AEHRLFERLFEDY---NEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKAL--LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSN-LNCFSRAESKGCKEGYPC-QDGMCGYCHHRRIKISNFSANLTRSSSS 425
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: CHRNA7 (cholinergic receptor nicotinic alpha 7 subunit [Source:HGNC Symbol;Acc:HGNC:1960]) HSP 1 Score: 200.675 bits (509), Expect = 2.958e-56 Identity = 134/381 (35.17%), Postives = 209/381 (54.86%), Query Frame = 1
Query: 184 YASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPIS 1317
+ S+ GE +KL +++K+Y + RPV N + V + L+L+QIM +DEKNQ+LT N+W + +W D L WN + V+ VR P +IW PDI+LYN ADER V VN G ++PP IFKS+C ID+ FPFD Q C LKFGSW+Y G LD+Q+ E DI Y + EW ++ P +R PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ MI++ LS +++IV+ + +P W R +N + L MK+ + +++ + H +R+ + E P P S
Sbjct: 18 HVSLQGEFQRKLYKELVKNYNP---LERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQM----QEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRP-----------GEDKVRPACQHKQRRCSLASVEMSAVAPPPAS 380
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Match: AC243734.1 (cholinergic receptor nicotinic alpha 7 subunit [Source:NCBI gene;Acc:1139]) HSP 1 Score: 200.675 bits (509), Expect = 2.958e-56 Identity = 134/381 (35.17%), Postives = 209/381 (54.86%), Query Frame = 1
Query: 184 YASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPIS 1317
+ S+ GE +KL +++K+Y + RPV N + V + L+L+QIM +DEKNQ+LT N+W + +W D L WN + V+ VR P +IW PDI+LYN ADER V VN G ++PP IFKS+C ID+ FPFD Q C LKFGSW+Y G LD+Q+ E DI Y + EW ++ P +R PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ MI++ LS +++IV+ + +P W R +N + L MK+ + +++ + H +R+ + E P P S
Sbjct: 18 HVSLQGEFQRKLYKELVKNYNP---LERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQM----QEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRP-----------GEDKVRPACQHKQRRCSLASVEMSAVAPPPAS 380
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-16 (Acetylcholine receptor subunit alpha-type acr-16 [Source:UniProtKB/Swiss-Prot;Acc:P48180]) HSP 1 Score: 193.356 bits (490), Expect = 4.431e-54 Identity = 115/316 (36.39%), Postives = 183/316 (57.91%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQ--PDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
E++L ++++Y + RPV N + V V +AL QI+ +DEKNQ++ VN W YTW D L+W+ + N+ +VR P KIW PD++LYN D + V G + W+PP IFK +C IDI FPFD Q C+ KFGSWTY G KLD+Q DI +Y + EW + R + D + PD+ F+L ++R + Y+ + L++PC+L +L+TL+ F LPP++ K+ L + + ++ L +++E +P S +VP LG ++ MI++T ST ++ V+NL++R + ++ W R
Sbjct: 23 ERRLYEDLMRNYNN---LERPVANHSEPVTVHLKVALQQIIDVDEKNQVVYVNAWLDYTWNDYNLVWDKAEYGNITDVRFPAGKIWKPDVLLYNSVDTNFDSTYQTNMIVYSTGLVHWVPPGIFKISCKIDIQWFPFDEQKCFFKFGSWTYDGYKLDLQ--PATGGFDISEYISNGEWALPLTTVERN--EKFYDCCPEPYPDVHFYLHMRRRTLYYGFNLIMPCILTTLMTLLGFTLPPDAGEKITLQITVLLSICFFLSIVSEMSPPTSEAVPLLGIFFTCCMIVVTASTVFTVYVLNLHYRTPETHDMGPWTR 331
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-9 (AcetylCholine Receptor [Source:UniProtKB/TrEMBL;Acc:Q18556]) HSP 1 Score: 194.126 bits (492), Expect = 6.124e-54 Identity = 116/332 (34.94%), Postives = 197/332 (59.34%), Query Frame = 1
Query: 175 LADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMP--PDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQL----YENFSE--IDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK 1146
L + S + E +L+ + +Y RPV N + +VV + L QI+ +DEKNQ++T+ W +Y W D L W+P + ++++R+P + IW PD++LYN ADE V V+Y G ++ +PP I K +C IDI+ FPFD Q C+LKFGSWTY G+ +D+++ +N S+ ID+ Y ++ EW +L PAR N+ D P L F+LI++R + Y+ L++P L+SL+T++ F LPP++ K+ L + I ++ L ++A+ TP S +VP +G ++ M++++ S +++V+NL+ R + + +LR+
Sbjct: 19 LTEVHSSADEYRLLADLRHNYDPYE---RPVANASEPLVVSVKIYLQQILDVDEKNQVITLVAWIEYQWTDYKLKWDPSEYGGIKDIRIPGNANAIWKPDVLLYNSADENFDSTYPVNYVVSYTGDVLQVPPGILKLSCKIDITYFPFDDQICHLKFGSWTYSGNFIDLRINGPEGKNISDEGIDVQYYVQNGEWNLLAVPARH--ETNIFDEQPYPSLFFYLIIQRRTLYYGLNLIIPSFLISLMTVLGFTLPPDAGEKITLEITILLSVCFFLSMVADMTPPTSEAVPLIGAFFSCCMLVVSASVVFTVLVLNLHNRKPETHEMSPFLRE 345
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-19 (AcetylCholine Receptor [Source:UniProtKB/TrEMBL;Acc:B3WFZ2]) HSP 1 Score: 193.356 bits (490), Expect = 1.592e-53 Identity = 110/304 (36.18%), Postives = 178/304 (58.55%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQP--DLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFR 1107
E++L K+ ++Y K + RPVRN + VVV G+ QI+ +DEK+QI+ NVW + +W D L W+P F N++EVR+P + IW PD++LYN D++ V V Y G + W+PPAI +S+C IDI+ FPFD Q C +KFGSWTY G D+ + I Y + EW +L ++R + + +P D+TF + ++R + Y+ + L++PC+L+S L L+ F LP + K+ LG+ IF++ + + ++AE P S ++P + Y+ M + S ++I +N + R
Sbjct: 25 ERRLYAKLAENYNK---LARPVRNESEAVVVLLGMDYQQILDIDEKHQIMNSNVWLRMSWTDHYLTWDPSEFGNIKEVRLPINNIWKPDVLLYNSVDQQFDSTWPVNAVVLYTGNVTWIPPAIIRSSCAIDIAYFPFDTQHCTMKFGSWTYSGFFTDLI----NTTISPATYKPNGEWELLGLTSQRSIF--FYECCPEPYYDVTFTVSIRRRTLYYGFNLLLPCMLISSLALLSFTLPADCGEKLNLGVTIFMSLCVFMIMVAEAMPQTSDALPLIQIYFSCIMFQVGASVVATVIALNFHHR 319
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-19 (AcetylCholine Receptor [Source:UniProtKB/TrEMBL;Acc:B3WFZ2]) HSP 1 Score: 193.356 bits (490), Expect = 1.723e-53 Identity = 110/304 (36.18%), Postives = 178/304 (58.55%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQP--DLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFR 1107
E++L K+ ++Y K + RPVRN + VVV G+ QI+ +DEK+QI+ NVW + +W D L W+P F N++EVR+P + IW PD++LYN D++ V V Y G + W+PPAI +S+C IDI+ FPFD Q C +KFGSWTY G D+ + I Y + EW +L ++R + + +P D+TF + ++R + Y+ + L++PC+L+S L L+ F LP + K+ LG+ IF++ + + ++AE P S ++P + Y+ M + S ++I +N + R
Sbjct: 31 ERRLYAKLAENYNK---LARPVRNESEAVVVLLGMDYQQILDIDEKHQIMNSNVWLRMSWTDHYLTWDPSEFGNIKEVRLPINNIWKPDVLLYNSVDQQFDSTWPVNAVVLYTGNVTWIPPAIIRSSCAIDIAYFPFDTQHCTMKFGSWTYSGFFTDLI----NTTISPATYKPNGEWELLGLTSQRSIF--FYECCPEPYYDVTFTVSIRRRTLYYGFNLLLPCMLISSLALLSFTLPADCGEKLNLGVTIFMSLCVFMIMVAEAMPQTSDALPLIQIYFSCIMFQVGASVVATVIALNFHHR 325
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Match: acr-25 (AcetylCholine Receptor [Source:UniProtKB/TrEMBL;Acc:Q65XS8]) HSP 1 Score: 192.586 bits (488), Expect = 2.642e-53 Identity = 106/318 (33.33%), Postives = 184/318 (57.86%), Query Frame = 1
Query: 190 SMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
+ E L T++L+DY+ + RP+ N + V+VK GL L QI+ +DEKNQ++ VN W K++WVD L W P+++ V ++R ++WTPD+++YN AD + + + V G + WMPP +++ +C I + FPFD Q C+LKFGSWT+ G KL++++ +N DI +Y ++ EW + +R + DL F +++R + Y+A+ L++PC+L+++LTLV F PP++ KM L + I ++ + +AE +P S +VPFL ++ + + + + +N + R K + + R
Sbjct: 22 TQKSEGDLYTELLEDYEP---LERPIENSSEAVMVKMGLVLQQIVDVDEKNQVVDVNAWLKFSWVDYSLKWKPEDYGGVTDLRFRKGQLWTPDVLMYNSADPQFDSRYASNLLVYPNGLVNWMPPGLYRLSCKIQVVWFPFDVQECFLKFGSWTFDGTKLNLEIDDN--GFDISNYMQNGEWTLEGTTVKRNIQYYQCCPEPYYDLVFTFVIRRRALYYAFNLILPCILITMLTLVGFTFPPDAGEKMSLQITIMLSICIFQNYVAELSPPTSEAVPFLSAFFAVCLFTCACCVTATTLALNFHHRTGKSHQMNNTFR 334
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha5 (gene:FBgn0028875 transcript:FBtr0300594) HSP 1 Score: 220.32 bits (560), Expect = 1.548e-61 Identity = 128/336 (38.10%), Postives = 202/336 (60.12%), Query Frame = 1
Query: 142 WIFVIFPSYITLADYASMSG--EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
WIF++ Y+ L+ ++G EK+L+ +L Y + RPV N D + + +GL L+QI+ +DEKNQ+L NVW K W D L WN ++ V+++R+PP +IW PD+++YN ADE V V G +++PP IFKSTC IDI+ FPFD Q C +KFGSWTY G +LD+QL + DI Y + EW +L P +R + D+TF +I++R + Y+ + L++PCVL++ + L+ F LPP+S K+ LG+ I ++ ++ L ++AET P+ S +VP LG Y+ M ++ S +I+++N + R + +W+R
Sbjct: 293 WIFLLI--YLNLSAKVCLAGYHEKRLLHDLLDPYNT---LERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGG-DISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIR 622
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha5 (gene:FBgn0028875 transcript:FBtr0080594) HSP 1 Score: 220.32 bits (560), Expect = 1.548e-61 Identity = 128/336 (38.10%), Postives = 202/336 (60.12%), Query Frame = 1
Query: 142 WIFVIFPSYITLADYASMSG--EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
WIF++ Y+ L+ ++G EK+L+ +L Y + RPV N D + + +GL L+QI+ +DEKNQ+L NVW K W D L WN ++ V+++R+PP +IW PD+++YN ADE V V G +++PP IFKSTC IDI+ FPFD Q C +KFGSWTY G +LD+QL + DI Y + EW +L P +R + D+TF +I++R + Y+ + L++PCVL++ + L+ F LPP+S K+ LG+ I ++ ++ L ++AET P+ S +VP LG Y+ M ++ S +I+++N + R + +W+R
Sbjct: 293 WIFLLI--YLNLSAKVCLAGYHEKRLLHDLLDPYNT---LERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGG-DISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIR 622
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha5 (gene:FBgn0028875 transcript:FBtr0309867) HSP 1 Score: 220.32 bits (560), Expect = 1.548e-61 Identity = 128/336 (38.10%), Postives = 202/336 (60.12%), Query Frame = 1
Query: 142 WIFVIFPSYITLADYASMSG--EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
WIF++ Y+ L+ ++G EK+L+ +L Y + RPV N D + + +GL L+QI+ +DEKNQ+L NVW K W D L WN ++ V+++R+PP +IW PD+++YN ADE V V G +++PP IFKSTC IDI+ FPFD Q C +KFGSWTY G +LD+QL + DI Y + EW +L P +R + D+TF +I++R + Y+ + L++PCVL++ + L+ F LPP+S K+ LG+ I ++ ++ L ++AET P+ S +VP LG Y+ M ++ S +I+++N + R + +W+R
Sbjct: 293 WIFLLI--YLNLSAKVCLAGYHEKRLLHDLLDPYNT---LERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGG-DISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIR 622
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha5 (gene:FBgn0028875 transcript:FBtr0474155) HSP 1 Score: 219.935 bits (559), Expect = 3.292e-61 Identity = 128/336 (38.10%), Postives = 204/336 (60.71%), Query Frame = 1
Query: 142 WIFVIFPSYITLADYASMSG--EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
WIF++ Y+ L+ ++G EK+L+ +L Y + RPV N D + + +GL L+QI+ +DEKNQ+L NVW K W D L WN ++ V+++R+PP +IW PD+++YN ADE V V G +++PP IFKSTC IDI+ FPFD Q C +KFGSWTY G +LD+QL ++ + DI Y + EW +L P +R + D+TF +I++R + Y+ + L++PCVL++ + L+ F LPP+S K+ LG+ I ++ ++ L ++AET P+ S +VP LG Y+ M ++ S +I+++N + R + +W+R
Sbjct: 293 WIFLLI--YLNLSAKVCLAGYHEKRLLHDLLDPYNT---LERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQL-QDETGGDISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIR 622
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Match: nAChRalpha7 (gene:FBgn0086778 transcript:FBtr0301835) HSP 1 Score: 211.46 bits (537), Expect = 6.669e-60 Identity = 122/325 (37.54%), Postives = 193/325 (59.38%), Query Frame = 1
Query: 169 ITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
I + A+ EK+L+ +L +Y + RPV N D + + +GL L+QI+ +DEKNQ+L N+W K W D L WN F V ++R+PP ++W PD+++YN ADE V V G +++PP IFKSTC IDI+ FPFD Q C +KFGSWTY G +LD+QL + DI + + EW +L P +R + D+TF ++++R + Y+ + L+VPCVL++ + L+ F LPP+S K+ LG+ I ++ ++ L ++AET P+ S +VP LG Y+ M ++ S +I+++N + R + +W+R
Sbjct: 50 IMIPACAAGPHEKRLLHALLDNYNS---LERPVVNESDPLQLSFGLTLMQIIDVDEKNQLLITNIWLKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYATNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGG-DISSFITNGEWDLLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHEMSEWIR 370
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: si:ch73-380n15.2 (si:ch73-380n15.2 [Source:ZFIN;Acc:ZDB-GENE-090313-161]) HSP 1 Score: 211.846 bits (538), Expect = 1.951e-60 Identity = 130/339 (38.35%), Postives = 200/339 (59.00%), Query Frame = 1
Query: 133 VIVWIFVIFPSYITLADYASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
IV+ IF SYI S+ GE +KL +++++Y + + RPV N +VV+ GL L+QI+ +DEKNQ+L N W + W D L WNP N+ V+ +R P ++IWTPDI+LYN ADER V VN G ++PP I KSTC ID+ FPFD Q C LKFGSWT+ G LD+Q+ +DI Y + EW ++ P +R L PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ M+++ LS ++++V+ + + +P W+R
Sbjct: 3 CIVYFLSIFLSYI-----GSLQGEYQRKLYKELMENYNR---LERPVLNDSAPIVVELGLTLLQIIDVDEKNQVLITNAWLQLGWTDVYLSWNPDNYPGVQTLRFPSNQIWTPDILLYNSADERFDATFHTNVLVNASGACQYIPPGILKSTCYIDVRWFPFDIQKCDLKFGSWTHNGWLLDLQM----QAVDISTYIPNGEWDLVGVPGKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGLSVVVTVLVLQFHHHDPQGGKMPRWVR 329
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: si:ch73-380n15.2 (si:ch73-380n15.2 [Source:ZFIN;Acc:ZDB-GENE-090313-161]) HSP 1 Score: 211.846 bits (538), Expect = 2.207e-60 Identity = 131/342 (38.30%), Postives = 201/342 (58.77%), Query Frame = 1
Query: 124 LIGVIVWIFVIFPSYITLADYASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
L IV+ IF SYI S+ GE +KL +++++Y + + RPV N +VV+ GL L+QI+ +DEKNQ+L N W + W D L WNP N+ V+ +R P ++IWTPDI+LYN ADER V VN G ++PP I KSTC ID+ FPFD Q C LKFGSWT+ G LD+Q+ +DI Y + EW ++ P +R L PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ M+++ LS ++++V+ + + +P W+R
Sbjct: 7 LPNCIVYFLSIFLSYI-----GSLQGEYQRKLYKELMENYNR---LERPVLNDSAPIVVELGLTLLQIIDVDEKNQVLITNAWLQLGWTDVYLSWNPDNYPGVQTLRFPSNQIWTPDILLYNSADERFDATFHTNVLVNASGACQYIPPGILKSTCYIDVRWFPFDIQKCDLKFGSWTHNGWLLDLQM----QAVDISTYIPNGEWDLVGVPGKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGLSVVVTVLVLQFHHHDPQGGKMPRWVR 336
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrna4b (cholinergic receptor, nicotinic, alpha 4b [Source:ZFIN;Acc:ZDB-GENE-090505-3]) HSP 1 Score: 213.001 bits (541), Expect = 6.803e-60 Identity = 130/339 (38.35%), Postives = 206/339 (60.77%), Query Frame = 1
Query: 145 IFVIFPSYITLADYASMS-----GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK 1146
+F + ++ LA SMS E++L+ + +Y K + RPV N+ D V+V +GL++ Q++ +DEKNQ++T NVW K W D L WNP+ + NV +R+P + IW PDIVLYN AD V Y GRI W PPAI+KS+C ID++ FPFD Q+C +KFGSWTY D+ I L S++D +DY ES EW+I++ + K PD+T+ I++R ++ L++PC+L+S LT+++F+LP E K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R + ++P W+R+
Sbjct: 7 VFFLLCVHLQLAYVFSMSPAHAHAEERLLQSLFINYNK---LSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWNDYKLRWNPEEYENVTSIRIPSEIIWRPDIVLYNNADGDFAVTHLTKAQVFYNGRIKWKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTY--DRAKIDLISMASDVDQMDYWESGEWVIINAVGTYNIKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHSMPHWVRQ 340
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: si:ch73-380n15.2 (si:ch73-380n15.2 [Source:ZFIN;Acc:ZDB-GENE-090313-161]) HSP 1 Score: 210.305 bits (534), Expect = 7.380e-60 Identity = 129/339 (38.05%), Postives = 200/339 (59.00%), Query Frame = 1
Query: 133 VIVWIFVIFPSYITLADYASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
IV+ IF S+I S+ GE +KL +++++Y + + RPV N +VV+ GL L+QI+ +DEKNQ+L N W + W D L WNP N+ V+ +R P ++IWTPDI+LYN ADER V VN G ++PP I KSTC ID+ FPFD Q C LKFGSWT+ G LD+Q+ +DI Y + EW ++ P +R L PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ M+++ LS ++++V+ + + +P W+R
Sbjct: 5 CIVYFLSIFLSHI-----GSLQGEYQRKLYKELMENYNR---LERPVLNDSAPIVVELGLTLLQIIDVDEKNQVLITNAWLQLGWTDVYLSWNPDNYPGVQTLRFPSNQIWTPDILLYNSADERFDATFHTNVLVNASGACQYIPPGILKSTCYIDVRWFPFDIQKCDLKFGSWTHNGWLLDLQM----QAVDISTYIPNGEWDLVGVPGKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGLSVVVTVLVLQFHHHDPQGGKMPRWVR 331
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2]) HSP 1 Score: 209.149 bits (531), Expect = 2.367e-59 Identity = 126/338 (37.28%), Postives = 203/338 (60.06%), Query Frame = 1
Query: 139 VWIFVIFPSYI--TLAD-YASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
+W+ ++ ++ L+D + E KL K+ Y K RPVRN+ D V+VK+GL++ Q++ +DEKNQ++T NVW K W D L WNP F NV +R+P + IW PDIVLYN AD + + G++ W+PPAI+KS+C ID++ FPFD Q+C +KFGSWTY K+D++ E +D+ DY ES EW I++ K + PD+T+F I++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R ++P W+
Sbjct: 13 LWMLILIADHVQPVLSDGWIHAHAEDKLFHKLFIGYNKWS---RPVRNISDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPSEFDNVTSIRVPSEMIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVSWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQIEG--TVDLKDYWESGEWAIINAVGTYNTKKYDCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHSMPRWVH 345
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: ENSXETT00000021532.1 (cholinergic receptor, nicotinic alpha 7 [Source:NCBI gene;Acc:100493611]) HSP 1 Score: 208.764 bits (530), Expect = 3.660e-59 Identity = 128/323 (39.63%), Postives = 196/323 (60.68%), Query Frame = 1
Query: 187 ASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQP--DLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
S+ GE +KL +++K+Y + RPV N + V +G++L+QIM +DEKNQ+LT N+W + W+D L WN + V+ VR P +IW PDI+LYN ADER V VN G ++PP IFKS+C ID+ FPFD Q C LKFGSWTY G LD+Q+ E+ DI +Y + EW ++ P +R +N + +P D+TF +I++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ MI++ LS +++IV+ + N+P W R
Sbjct: 21 VSLQGEYQRKLYKELMKNYNP---LERPVLNDSHPLTVYFGMSLMQIMDVDEKNQVLTTNIWLQMQWIDYYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDASFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDIQKCNLKFGSWTYGGWSLDLQMLES----DISEYISNGEWDLVGVPGKRS--ENYYECCKEPYLDVTFTIIMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPHGGNMPKWTR 334
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: CHRNA2 (cholinergic receptor, nicotinic alpha 2 [Source:NCBI gene;Acc:100495873]) HSP 1 Score: 206.838 bits (525), Expect = 1.845e-58 Identity = 154/545 (28.26%), Postives = 273/545 (50.09%), Query Frame = 1
Query: 112 CHMRLIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWV 1743
C R + ++ WI + ++ + E++L ++ Y + RPV N D V+VK+GL++ Q++ +DEKNQ++T NVW K W D L WNP ++ NV +R+P + IW PDIVLYN AD + + G++ W+PPAI+KS+C ID++ FPFD QSC +KFGSWTY LD++ E +D+ DY ES EW I++ + K PD+T++ I++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R +P W+R ++ + L MK+ + E+K S + K E + + A ++ +++++ + + Q R+ Y N V+ S PR+S ++ +S+ LL ++ + + + + + +D + ++W+ VAMV+DR+F W+
Sbjct: 4 CMGRTLSILCWIITF--TTVSCMERTRTHAEERLFKRLFTGY---NRWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPADYDNVTSIRVPSEMIWIPDIVLYNNADGEFAITHMTKAHLFHNGKVKWVPPAIYKSSCSIDVTFFPFDQQSCKMKFGSWTYDKAWLDLESMER--NVDLKDYWESGEWAIVNAVGKYNSKKYDCCTEIYPDITYYFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPLWVRSVFLDHIPRWLFMKRPPAPVEEVVSQYDAPELKLSTS--KYWMETDVDDK----------WAEPEAELQTCHVRHMTTHNQSLQYRYDY---NHHVSG----GQSQTPRHS----------------------------------STETAESSFLLSPSVLKALEGVHYIANHLRAEDADFSVKEDWKYVAMVIDRIFLWM 488
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: CHRNA2 (cholinergic receptor, nicotinic alpha 2 [Source:NCBI gene;Acc:100495873]) HSP 1 Score: 204.142 bits (518), Expect = 1.207e-57 Identity = 123/344 (35.76%), Postives = 202/344 (58.72%), Query Frame = 1
Query: 112 CHMRLIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
C R + ++ WI + ++ + E++L ++ Y + RPV N D V+VK+GL++ Q++ +DEKNQ++T NVW K W D L WNP ++ NV +R+P + IW PDIVLYN AD + + G++ W+PPAI+KS+C ID++ FPFD QSC +KFGSWTY LD++ E +D+ DY ES EW I++ + K PD+T++ I++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R +P W+R
Sbjct: 4 CMGRTLSILCWIITF--TTVSCMERTRTHAEERLFKRLFTGY---NRWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPADYDNVTSIRVPSEMIWIPDIVLYNNADGEFAITHMTKAHLFHNGKVKWVPPAIYKSSCSIDVTFFPFDQQSCKMKFGSWTYDKAWLDLESMER--NVDLKDYWESGEWAIVNAVGKYNSKKYDCCTEIYPDITYYFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPLWVR 340
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: mppe1 (metallophosphoesterase 1 [Source:Xenbase;Acc:XB-GENE-987928]) HSP 1 Score: 198.749 bits (504), Expect = 8.134e-56 Identity = 125/369 (33.88%), Postives = 215/369 (58.27%), Query Frame = 1
Query: 175 LADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGM----KQVISSASSEKLHLIKSEIKNSRNHIKRQ 1266
L + + E +L +LK Y RPV NV D V++ + +++ Q++ +DE NQI+ N+W ++ W D L WNP ++ +E +R+P +IW PDIVLYN A V Y G + W+PPAIFKS+C ID++ FPFD+Q+C LKFGSWTY DK I L S+I++ ++ ES EW+I++ P + +K D+T+ L ++R ++ +++PC+L+S LT+++F+LP + K+ L M++ ++ ++ L ++ E PS S +P +G Y MI +TLS +++ V+N+++R K +P W+++ ++ K++ M KQ + L + SEI++ ++ + Q
Sbjct: 11 LLGFFCSNVEHRLYEDLLKSYNPYI---RPVMNVSDPVILHFEVSMSQLVKVDEVNQIMETNLWLRHLWNDYKLKWNPSDYGGIEFLRIPASQIWRPDIVLYNNAVGAFHVDDKAKAVVRYSGDVTWIPPAIFKSSCKIDVTFFPFDYQNCSLKFGSWTY--DKAKINLVMIGSKINLKEFWESGEWVIINAPGYKHEIKYNCCVEIYQDITYSLYIRRLPLFYTIYIIIPCLLISCLTILVFYLPSDCGEKVTLCMSVLLSLTVFLLVITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPHWVKRFFLHLLPKVMFMTRPEKQPYALKPQAYLDISSSEIRSCQDQLFYQ 374
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Match: mppe1 (metallophosphoesterase 1 [Source:Xenbase;Acc:XB-GENE-987928]) HSP 1 Score: 198.749 bits (504), Expect = 9.162e-56 Identity = 125/369 (33.88%), Postives = 215/369 (58.27%), Query Frame = 1
Query: 175 LADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGM----KQVISSASSEKLHLIKSEIKNSRNHIKRQ 1266
L + + E +L +LK Y RPV NV D V++ + +++ Q++ +DE NQI+ N+W ++ W D L WNP ++ +E +R+P +IW PDIVLYN A V Y G + W+PPAIFKS+C ID++ FPFD+Q+C LKFGSWTY DK I L S+I++ ++ ES EW+I++ P + +K D+T+ L ++R ++ +++PC+L+S LT+++F+LP + K+ L M++ ++ ++ L ++ E PS S +P +G Y MI +TLS +++ V+N+++R K +P W+++ ++ K++ M KQ + L + SEI++ ++ + Q
Sbjct: 16 LLGFFCSNVEHRLYEDLLKSYNPYI---RPVMNVSDPVILHFEVSMSQLVKVDEVNQIMETNLWLRHLWNDYKLKWNPSDYGGIEFLRIPASQIWRPDIVLYNNAVGAFHVDDKAKAVVRYSGDVTWIPPAIFKSSCKIDVTFFPFDYQNCSLKFGSWTY--DKAKINLVMIGSKINLKEFWESGEWVIINAPGYKHEIKYNCCVEIYQDITYSLYIRRLPLFYTIYIIIPCLLISCLTILVFYLPSDCGEKVTLCMSVLLSLTVFLLVITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPHWVKRFFLHLLPKVMFMTRPEKQPYALKPQAYLDISSSEIRSCQDQLFYQ 379
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna9 (cholinergic receptor, nicotinic, alpha polypeptide 9 [Source:MGI Symbol;Acc:MGI:1202403]) HSP 1 Score: 206.838 bits (525), Expect = 6.418e-59 Identity = 168/533 (31.52%), Postives = 253/533 (47.47%), Query Frame = 1
Query: 163 SYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGV-NATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIV 1758
S I + A+ +KL + + +DY RPV + + V + L QI +DE+NQILT +W + TW D L W+ + ++ +R+P D +W PDIVLYN AD+ E + V + Y G I W PAI KS+C +D++ FPFD Q C L FGSWTY G+++DI + D+ D+ E EW + PA + V+ + PD+TF L+LKR S+++ L++PCVL+S L + F+LP S K+ LG+ I +A ++ ++AE P AS +VP +G YY M LIT ST L+I+V+N++F + R +P W + + S+IL + V S S + Q+ ++ T+ +P +R+K E++ L + YQ QNTD SY RY L KN+ I K LK + N EW+ VA V+DR F W++F +V
Sbjct: 18 SGIKAVETANGKYAQKLFSDLFEDYSNAL---RPVEDTDAVLNVTLQVTLSQIKDMDERNQILTAYLWIRQTWHDAYLTWDRDQYDGLDSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRYDGLITWDSPAITKSSCVVDVTYFPFDSQQCNLTFGSWTYNGNQVDI--FNALDSGDLSDFIEDVEWEVQGMPAVKNVISYGCCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFYLPAASGEKVSLGVTILLAMTVFQLMVAEIMP-ASENVPLIGKYYIATMALITASTALTIMVMNIHFCGAEARPVPHWAKVVILKYMSRILFVYDVGESCLSPR---------------HSQEPEQITKVYGKLPGSNLKASRNKDLSRKKELRKLLKNDLGYQGGIP---QNTD---------------------SYCARYEA-------------------------------LTKNIEYIAKCLK-------DHKATNSKGSEWKKVAKVIDRFFMWIFFAMV 467
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna3 (cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887]) HSP 1 Score: 201.445 bits (511), Expect = 5.216e-57 Identity = 132/404 (32.67%), Postives = 228/404 (56.44%), Query Frame = 1
Query: 187 ASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLK-NMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSA--SSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
++ E +L + +DY +I RPV NV V++++ +++ Q++ +DE NQI+ N+W K W D L W P ++ VE +R+P +KIW PDIVLYN AD + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K N + Q D+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + + +E+ N N R K E H+ K++ NL+ SSS+
Sbjct: 24 SASEAEHRLFQYLFEDY---NEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQ-DITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSN-LNCFSRADSKSCKEGYPC-QDGTCGYCHHRRVKISNFSANLTRSSSS 419
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna3 (cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887]) HSP 1 Score: 201.445 bits (511), Expect = 9.803e-57 Identity = 132/404 (32.67%), Postives = 228/404 (56.44%), Query Frame = 1
Query: 187 ASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLK-NMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSA--SSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
++ E +L + +DY +I RPV NV V++++ +++ Q++ +DE NQI+ N+W K W D L W P ++ VE +R+P +KIW PDIVLYN AD + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K N + Q D+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + + +E+ N N R K E H+ K++ NL+ SSS+
Sbjct: 29 SASEAEHRLFQYLFEDY---NEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQ-DITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSN-LNCFSRADSKSCKEGYPC-QDGTCGYCHHRRVKISNFSANLTRSSSS 424
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna3 (cholinergic receptor, nicotinic, alpha polypeptide 3 [Source:MGI Symbol;Acc:MGI:87887]) HSP 1 Score: 201.445 bits (511), Expect = 9.952e-57 Identity = 132/404 (32.67%), Postives = 228/404 (56.44%), Query Frame = 1
Query: 187 ASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLK-NMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSA--SSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
++ E +L + +DY +I RPV NV V++++ +++ Q++ +DE NQI+ N+W K W D L W P ++ VE +R+P +KIW PDIVLYN AD + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K N + Q D+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + + +E+ N N R K E H+ K++ NL+ SSS+
Sbjct: 24 SASEAEHRLFQYLFEDY---NEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQ-DITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSTEEDAPKTRNFYGAELSN-LNCFSRADSKSCKEGYPC-QDGTCGYCHHRRVKISNFSANLTRSSSS 419
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Match: Chrna2 (cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) [Source:MGI Symbol;Acc:MGI:87886]) HSP 1 Score: 201.83 bits (512), Expect = 1.059e-56 Identity = 123/340 (36.18%), Postives = 201/340 (59.12%), Query Frame = 1
Query: 127 IGVIVWIFVIFPSYITLADYASMS-GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
I + +W ++ P+ LA S + E +L + Y + RPV N D V+V++GL++ Q++ +DEKNQ++T NVW K W D L W+P F N+ +R+P + IW PDIVLYN AD + + G + W+PPAI+KS+C ID++ FPFD Q+C +KFGSWTY K+D++ E +D+ DY ES EW I++ K PD+T++ +++R ++ L++PC+L+S LT+++F+LP E K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R N+P+W+R
Sbjct: 12 IHLYLWCLLLMPA--VLAQQGSHTHAEDRLFKHLFGGYNRWA---RPVPNTSDVVIVRFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMER--TVDLKDYWESGEWAIINATGTYNSKKYDCCAEIYPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVR 344
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P09482|ACHA4_CHICK (Neuronal acetylcholine receptor subunit alpha-4 OS=Gallus gallus OX=9031 GN=CHRNA4 PE=1 SV=1) HSP 1 Score: 214.927 bits (546), Expect = 1.035e-59 Identity = 133/345 (38.55%), Postives = 210/345 (60.87%), Query Frame = 1
Query: 154 IFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQ 1185
IFP++ + A E++L+ K+ Y K RPV N+ D V+V++GL++ Q++ +DEKNQ++T NVW K W D L W+P+ + NV +R+P + IW PDIVLYN AD + Y GRI WMPPAI+KS+C ID++ FPFD Q+C +KFGSWTY DK I L S +D +DY ES EW+I++ K PD+T+ I++R ++ L++PC+L+S LT+++F+LP E K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R + +PDW+R+ ++ ++L MK+
Sbjct: 18 IFPAFGHVETRAH--AEERLLKKLFSGY---NKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPQEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRIKWMPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTY--DKAKIDLVSMHSHVDQLDYWESGEWVIINAVGNYNSKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPRTHTMPDWVRRVFLDIVPRLLFMKR 355
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|Q07263|ACHA3_BOVIN (Neuronal acetylcholine receptor subunit alpha-3 OS=Bos taurus OX=9913 GN=CHRNA3 PE=2 SV=1) HSP 1 Score: 206.838 bits (525), Expect = 6.362e-58 Identity = 132/404 (32.67%), Postives = 236/404 (58.42%), Query Frame = 1
Query: 187 ASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISS-ASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
++ E +L ++ +DY +I RPV NV D V++++ +++ Q++ +DE NQI+ N+W K W D L WNP ++ E +R+P +KIW PDIVLYN A D ++ +K + + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K PD+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ ++++ S ++ N R + K E + H+ +K++ NL+ SSS+
Sbjct: 20 STSDAEHRLFERLFEDY---NEIIRPVANVSDPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYDGAEFMRVPAEKIWKPDIVLYNNAVGDFQVDDKTKAL--LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPAWVKTIFLNLLPRVMFMTRPASNEGNTQRPRPFYSAELSNLNCFSRIESKVCKEGYPC-QDGLCGYCHHRRAKISNFSANLTRSSSS 415
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P43144|ACHA9_RAT (Neuronal acetylcholine receptor subunit alpha-9 OS=Rattus norvegicus OX=10116 GN=Chrna9 PE=1 SV=2) HSP 1 Score: 206.068 bits (523), Expect = 8.455e-58 Identity = 165/519 (31.79%), Postives = 248/519 (47.78%), Query Frame = 1
Query: 205 KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGV-NATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIV 1758
+KL + + +DY RPV + + V + L QI +DE+NQILT +W + TW D L W+ + ++ +R+P D +W PDIVLYN AD+ E + V + Y G I W PAI KS+C +D++ FPFD Q C L FGSWTY G+++DI + D+ D+ E EW + PA + V+ + PD+TF L+LKR S+++ L++PCVL+S L + F+LP S K+ LG+ I +A ++ ++AE P AS +VP +G YY M LIT ST L+I+V+N++F + R +P W + + S+IL + V S S + Q+ ++ T+ +P +R+K E++ L + YQ QNTD SY RY L KN+ I K LK + N EW+ VA V+DR F W++F +V
Sbjct: 32 QKLFSDLFEDYSSAL---RPVEDTDAVLNVTLQVTLSQIKDMDERNQILTAYLWIRQTWHDAYLTWDRDQYDRLDSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRYDGLITWDSPAITKSSCVVDVTYFPFDSQQCNLTFGSWTYNGNQVDI--FNALDSGDLSDFIEDVEWEVHGMPAVKNVISYGCCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFYLPAASGEKVSLGVTILLAMTVFQLMVAEIMP-ASENVPLIGKYYIATMALITASTALTIMVMNIHFCGAEARPVPHWAKVVILKYMSRILFVYDVGESCLSPR---------------HSQEPEQVTKVYSKLPESNLKTSRNKDLSRKKEVRKLLKNDLGYQGGIP---QNTD---------------------SYCARYEA-------------------------------LTKNIEYIAKCLK-------DHKATNSKGSEWKKVAKVIDRFFMWIFFAMV 467
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P32297|ACHA3_HUMAN (Neuronal acetylcholine receptor subunit alpha-3 OS=Homo sapiens OX=9606 GN=CHRNA3 PE=1 SV=4) HSP 1 Score: 206.453 bits (524), Expect = 1.131e-57 Identity = 134/401 (33.42%), Postives = 231/401 (57.61%), Query Frame = 1
Query: 199 GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEK--LHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSN 1386
E +L ++ +DY + I RPV NV D V++ + +++ Q++ +DE NQI+ N+W K W D L WNP ++ E +R+P KIW PDIVLYN A D ++ +K + + Y G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSW+Y K+D+ L S +++ DY ES EW I+ P + +K PD+T+ L ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R +P W++ +N +++ M + S+ + + L +E+ N N R + K E + H+ K++ NL+ SSS+
Sbjct: 34 AEHRLFERLFEDYNE---IIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKAL--LKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIG--SSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSN-LNCFSRAESKGCKEGYPC-QDGMCGYCHHRRIKISNFSANLTRSSSS 425
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Match: sp|P09480|ACHA2_CHICK (Neuronal acetylcholine receptor subunit alpha-2 OS=Gallus gallus OX=9031 GN=CHRNA2 PE=2 SV=1) HSP 1 Score: 205.297 bits (521), Expect = 5.745e-57 Identity = 125/340 (36.76%), Postives = 200/340 (58.82%), Query Frame = 1
Query: 124 LIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
+I ++VW FV + E +L + Y + RPV N D V+VK+GL++ Q++ +DEKNQ++T NVW K W D L WNP++F NV +R+P + IW PDIVLYN AD + G++ W+PPAI+KS+C ID++ FPFD Q+C +KFGSWTY K+D++ E+ +D+ DY ES EW I++ R K PD+TF+ +++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R +P W+R
Sbjct: 8 IIPLLVWCFVTLQAATREQKQPHGFAEDRLFKHLFTGYNRWS---RPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWSDYKLRWNPEDFDNVTSIRVPSEMIWIPDIVLYNNADGEFAVTHMTKAHLFSNGKVKWVPPAIYKSSCSIDVTYFPFDQQNCKMKFGSWTYDKAKIDLENMEH--HVDLKDYWESGEWAIINAIGRYNSKKYDCCTEIYPDITFYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPSTHTMPHWVR 342
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A5K4F4B2 (Putative nachr subunit OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 436.417 bits (1121), Expect = 1.047e-141 Identity = 275/611 (45.01%), Postives = 394/611 (64.48%), Query Frame = 1
Query: 136 IVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQV---ISSASSEKLHLIKSEIKNS--RNHIKRQQEKETTESRETIPTPISPRAR------------------------HKSSKMAMEMQNLS----GSSSNYQTRF--------QYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYE 1845
++WI++I + + ++ S EK+LI +++ +Y+K GKIGRPV+N +D VVV YGL+L Q++ LDEKNQILT+NVWAKYTW+D++L W+P N+SN+ EVR+PP IWTPDIVL+NYADERL EKRDVM++V+Y+GR+ W PPAIFKS C IDI NFPFD+Q C+LKF SWTY ++LD+Q +N +E+DI YT SNEW ++ +PA RFV+ + + PDLTFFL LKRN A++ YLLV PCVLLS++T VIFWLPP+ PAKMIL MNIFVAFSLLL++LA +TPSAST VP+LGY+YCLNM L++LSTF S IV++L+F+ K +++P WL K V + +L + ++ + + +++KL ++++IKN+ + +K + +T S + +P P +P++ + + ++ Q LS GS +Y T YP QN Y H IP + S E + A K + +++S S I LE NL ++ ALK LM KIA+KD + +EWR+V M +DRLF W Y +VI++G+ LL PRG ++++I+ + Y+
Sbjct: 4 LLWIWLI--CLVFIPEHVIPSSEKRLIKQLIDNYEKAGKIGRPVKNTKDRVVVGYGLSLFQLLDLDEKNQILTINVWAKYTWIDQLLRWDPANYSNIREVRIPPSVIWTPDIVLHNYADERLKEKRDVMLNVDYMGRVFWSPPAIFKSNCPIDIKNFPFDYQHCFLKFASWTYNSEQLDLQFLDNRTEVDIDQYTSSNEWSLVARPAYRFVMLSDECGKEIPDLTFFLFLKRNPAFYGYLLVFPCVLLSVITTVIFWLPPQIPAKMILSMNIFVAFSLLLKILAVSTPSASTIVPYLGYFYCLNMTLLSLSTFASTIVVHLHFQRVKSKHVPPWLIKVVRFFATLLHVSKLEPPVVADANQKLSAVEAQIKNAKMKQSVKSENLLDTWSSNQQLP-PSNPQSNLIHSVGVETQWDYNKISSPEEYLLNPNELSVLQKQQLSMLSDGSRISYCTSVNGCQFSSHHYPNQN-------YLQHKSIPWGDYAMNQSNE---------SGTAKIHDKMLKKFKQSKQQSRGSTIKLEFNLGDLCNALKQLMLKIAKKDEIAQRDREWRLVLMTIDRLFCWFYSTVVIVAGVYLLFPRGRSHTVNEIIAMHQEAYD 595
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A5K4F410 (Putative nachr subunit OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 434.876 bits (1117), Expect = 6.681e-141 Identity = 272/591 (46.02%), Postives = 383/591 (64.81%), Query Frame = 1
Query: 196 SGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQV---ISSASSEKLHLIKSEIKNS--RNHIKRQQEKETTESRETIPTPISPRAR------------------------HKSSKMAMEMQNLS----GSSSNYQTRF--------QYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYE 1845
S EK+LI +++ +Y+K GKIGRPV+N +D VVV YGL+L Q++ LDEKNQILT+NVWAKYTW+D++L W+P N+SN+ EVR+PP IWTPDIVL+NYADERL EKRDVM++V+Y+GR+ W PPAIFKS C IDI NFPFD+Q C+LKF SWTY ++LD+Q +N +E+DI YT SNEW ++ +PA RFV+ + + PDLTFFL LKRN A++ YLLV PCVLLS++T VIFWLPP+ PAKMIL MNIFVAFSLLL++LA +TPSAST VP+LGY+YCLNM L++LSTF S IV++L+F+ K +++P WL K V + +L + ++ + + +++KL ++++IKN+ + +K + +T S + +P P +P++ + + ++ Q LS GS +Y T YP QN Y H IP + S E + A K + +++S S I LE NL ++ ALK LM KIA+KD + +EWR+V M +DRLF W Y +VI++G+ LL PRG ++++I+ + Y+
Sbjct: 35 SSEKRLIKQLIDNYEKAGKIGRPVKNTKDRVVVGYGLSLFQLLDLDEKNQILTINVWAKYTWIDQLLRWDPANYSNIREVRIPPSVIWTPDIVLHNYADERLKEKRDVMLNVDYMGRVFWSPPAIFKSNCPIDIKNFPFDYQHCFLKFASWTYNSEQLDLQFLDNRTEVDIDQYTSSNEWSLVARPAYRFVMLSDECGKEIPDLTFFLFLKRNPAFYGYLLVFPCVLLSVITTVIFWLPPQIPAKMILSMNIFVAFSLLLKILAVSTPSASTIVPYLGYFYCLNMTLLSLSTFASTIVVHLHFQRVKSKHVPPWLIKVVRFFATLLHVSKLEPPVVADANQKLSAVEAQIKNAKMKQSVKSENLLDTWSSNQQLP-PSNPQSNLIHSVGVETQWDYNKISSPEEYLLNPNELSVLQKQQLSMLSDGSRISYCTSVNGCQFSSHHYPNQN-------YLQHKSIPWGDYAMNQSNE---------SGTAKIHDKMLKKFKQSKQQSRGSTIKLEFNLGDLCNALKQLMLKIAKKDEIAQRDREWRLVLMTIDRLFCWFYSTVVIVAGVYLLFPRGRSHTVNEIIAMHQEAYD 608
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A095AWG0 (Neuronal acetylcholine receptor subunit alpha-4 OS=Schistosoma haematobium OX=6185 GN=MS3_07391 PE=3 SV=1) HSP 1 Score: 434.491 bits (1116), Expect = 7.685e-141 Identity = 274/614 (44.63%), Postives = 392/614 (63.84%), Query Frame = 1
Query: 136 IVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQV---ISSASSEKLHLIKSEIKNS--RNHIKRQQEKETTESRETIPTPISPRARHKSSK------------------------MAMEMQNLS----GSSSNYQT--------RFQYPRQNTDVTDIPYTAHSDIPRNSF---NPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYE 1845
++WI+++ + + ++ S EK+LI +++ +Y+K GKIGRPV+N +D VVV YGL+L Q++ LDEKNQILT+NVWAKYTW+D++L W+P N+SN+ EVR+PP IWTPDIVLYNYADERL EKRDVM++V+Y+GR+ W PPAIFKS C IDI NFPFD+Q C+LKF SWTY ++LD+Q +N +E+DI YT SNEW ++ +PA RFV+ + + PDLTFFL LKRN A++ YLLV PCVLLS++T VIFWLPP+ PAKMIL MNIFVAFSLLL++LA +TPSAST VP+LGY+YCLNM L++LSTF S IV++L+F+ K +++P WL K V + +L + ++ + + +++KL +++++IKN+ + IK + +T S + +P P +P++ S ++ Q LS GS +Y T YP QN Y IP + P S + K +++S +S I L NL ++ ALK LM KIA+KD + +EWR+V M +DRLF W Y ++VI++G+ LL PRG ++++I+ + Y+
Sbjct: 4 LIWIWLV--CLVFIPEHVIPSSEKRLIKQLIDNYEKAGKIGRPVKNTKDRVVVGYGLSLFQLLDLDEKNQILTINVWAKYTWIDQLLRWDPANYSNIREVRIPPSVIWTPDIVLYNYADERLKEKRDVMLNVDYMGRVFWSPPAIFKSNCPIDIKNFPFDYQHCFLKFASWTYNSEQLDLQFLDNRTEVDIDQYTSSNEWSLVARPAYRFVMLSDECGKEIPDLTFFLFLKRNPAFYGYLLVFPCVLLSVITTVIFWLPPQIPAKMILSMNIFVAFSLLLKILAVSTPSASTIVPYLGYFYCLNMTLLSLSTFASTIVVHLHFQRVKSKHVPPWLIKVVRFFATLLHVSKLEPPVVADTNQKLSVVEAQIKNAKMKQSIKSENLLDTWSSNQQLP-PSNPQSNLIHSAGVETQWDYNKISSTEEYLLNPNELSVLQKQQLSMLSDGSQISYSTGVNGCQFPSHHYPNQN-------YLQQKSIPWGDYATNQPNKSGSVK------------IHDKLLKKFLQSKQQSRRSTIQLGFNLGDLCNALKQLMLKIAKKDEIAQRDREWRLVLMTIDRLFCWFYSIVVIVAGVYLLFPRGRSHTVNEIIAMHQEAYD 595
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A1I8G3D5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 413.305 bits (1061), Expect = 2.690e-134 Identity = 232/374 (62.03%), Postives = 292/374 (78.07%), Query Frame = 1
Query: 175 LADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRE 1296
+A+ ++S EK LI K+L DYK G GRPV+N D V V++GL LIQI+ LDEKNQ+LT+ VW++++W D++L WN ++ V EVR+P +WTPDIVLYNYADERL E+R+VM+ N+ GR+IW+PPAI+KSTC +DI+NFPFD QSC+LKFGSWTY G KLDIQ Y+N S I DY +SNEW I+ PA R V N PDLTFFL LKRN + AYLLV+PCVLLS LTLVIFWLPPESPAKM+LGMNIFVAF LLL LLAE+TPSASTSVP++G+YYCLNM+LITLSTFLS+IVINLYFR DKR LP WL+K + +K+L M+QVI+ + +EKL LI+SEIK++R+H K++++ + E
Sbjct: 8 IAESIALSDEKTLIKKILLDYKTAGVDGRPVQNTTDVVTVQFGLGLIQILDLDEKNQVLTIKVWSRFSWTDQLLQWNATQYNGVSEVRIPTRDVWTPDIVLYNYADERLKEQREVMIVTNHTGRVIWIPPAIYKSTCQVDITNFPFDQQSCHLKFGSWTYDGFKLDIQFYQNKSNISTDDYIQSNEWHIVALPATRNVKFYPCCNEPYPDLTFFLFLKRNPEFFAYLLVLPCVLLSSLTLVIFWLPPESPAKMLLGMNIFVAFFLLLLLLAESTPSASTSVPYIGFYYCLNMLLITLSTFLSVIVINLYFRGDKRNRLPGWLKKCIEFLAKMLCMRQVITKSETEKLRLIRSEIKHNRHHNKKKKQGRRAQVVE 381
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Match: A0A267GKY0 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005119g9 PE=3 SV=1) HSP 1 Score: 411.764 bits (1057), Expect = 1.918e-131 Identity = 231/369 (62.60%), Postives = 289/369 (78.32%), Query Frame = 1
Query: 190 SMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRE 1296
++S EK LI K+L DYK G GRPV+N D V V++GL LIQI+ LDEKNQ+LT+ VW++++W D++L WN ++ V EVR+P +WTPDIVLYNYADERL E+R+VM+ N+ GR+IW+PPAI+KSTC +DI+NFPFD QSC+LKFGSWTY G KLDIQ Y+N S I DY +SNEW I+ PA R V N PDLTFFL LKRN + AYLLV+PCVLLS LTLVIFWLPPESPAKM+LGMNIFVAF LLL LLAE+TPSASTSVP++G+YYCLNM+LITLSTFLS+IVINLYFR DKR LP WL+K + +K+L M+QVI+ + +EKL LI+SEIK++R+H K++++ + E
Sbjct: 81 ALSDEKTLIKKILLDYKTAGVDGRPVQNTTDVVTVQFGLGLIQILDLDEKNQVLTIKVWSRFSWTDQLLQWNATQYNGVSEVRIPTRDVWTPDIVLYNYADERLKEQREVMIVTNHTGRVIWIPPAIYKSTCQVDITNFPFDQQSCHLKFGSWTYDGFKLDIQFYQNKSNISTDDYIQSNEWHIVALPATRNVKFYPCCNEPYPDLTFFLFLKRNPEFFAYLLVLPCVLLSSLTLVIFWLPPESPAKMLLGMNIFVAFFLLLLLLAESTPSASTSVPYIGFYYCLNMLLITLSTFLSVIVINLYFRGDKRNRLPGWLKKCIEFLAKMLCMRQVITKSETEKLRLIRSEIKHNRHHNKKKKQGRRAQVVE 449 HSP 2 Score: 75.485 bits (184), Expect = 2.133e-10 Identity = 38/80 (47.50%), Postives = 59/80 (73.75%), Query Frame = 1
Query: 1606 LEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKS-MDQILEEYARKY 1842
+E +L EIR++++NLM ++A+KD ++A+EWRMVA+VLDR+FFW Y VI+I +G LL+P S +++ +EEY Y
Sbjct: 564 MESDLREIRRSIRNLMMRLAQKDATQKVAREWRMVALVLDRIFFWCYLVIIITAGFTLLMPTSPAASRIEETIEEYKADY 643
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2]) HSP 1 Score: 211.846 bits (538), Expect = 1.953e-60 Identity = 131/361 (36.29%), Postives = 210/361 (58.17%), Query Frame = 1
Query: 175 LADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNH 1254
L D+ E +L K+ Y K RPVRN+ D V+VK+GL++ Q++ +DEKNQ++T NVW K W D L W P ++ NV +R+P + IW PDIVLYN AD + + G+++W+PPAI+KS+C ID++ FPFD Q+C +KFGSWTY K+D++ EN +D+ DY ES EW I++ K + PD+T+F I++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R +P W++ + + L MK+ + +L L E K N
Sbjct: 14 LCDWTHAHAEDQLFKKLFSGYNKWS---RPVRNITDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFHTGKVLWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLERIEN--TVDLKDYWESGEWAIVNAVGTYNTKKYDCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHKMPGWVQSIFLGFIPRWLFMKRPAPDQTRRRLLLNAQEKKQVPNQ 369
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: si:ch73-380n15.2 (si:ch73-380n15.2 [Source:ZFIN;Acc:ZDB-GENE-090313-161]) HSP 1 Score: 211.46 bits (537), Expect = 3.849e-60 Identity = 128/331 (38.67%), Postives = 197/331 (59.52%), Query Frame = 1
Query: 157 FPSYITLADYASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
FPS + + S+ GE ++L ++L +Y + + RPV N +VV+ GL L+QI+ +DEKNQ+L N W + W D L WNP N+ V+ +R P ++IWTPDI+LYN ADER V VN G ++PP I KSTC ID+ FPFD Q C LKFGSWTY G LD+Q+ E+DI Y + EW ++ P +R L PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ M+++ LS ++++V+ + + +P W+R
Sbjct: 28 FPSAVLMN---SLQGEHQRRLYKELLTNYNR---LERPVANDSAPLVVELGLTLLQIIDVDEKNQVLITNAWLQLGWTDVYLTWNPDNYPGVQNLRFPSNQIWTPDILLYNSADERFDATFHTNVLVNASGTCQYIPPGILKSTCYIDVRWFPFDVQKCDLKFGSWTYNGWLLDLQM----QEVDISTYIPNGEWDLVGVPGKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGLSVVVTVLVLQFHHHDPQGGKMPKWVR 348
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna3 (neuronal acetylcholine receptor subunit alpha-3-like [Source:NCBI gene;Acc:103025148]) HSP 1 Score: 210.69 bits (535), Expect = 8.061e-60 Identity = 160/537 (29.80%), Postives = 273/537 (50.84%), Query Frame = 1
Query: 157 FPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLS-EIRKALKNLMTKIAEKDRGNRIAKE----WRMVAMVLDRLFFWVY 1746
F ++ +AS E +L + + Y + RPV NV D V+V++ +++ Q++ +DE NQI+ N+W ++ W D L W+PK+F VE +R+P ++IW PDIVLYN A D ++ +K ++ N G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSWTY K+D+ L S I++ D+ ES EW+I+ P + +K D+T+ L ++R ++ +++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R K +P W+R+ LG+ + + + ++ S + T P + PR S + S S + R R N + P D + P + + +S +L LS E+R+A+++ + IAE R AKE W+ VAMV+DR+F WV+
Sbjct: 8 FLFFLLPGGWAS-EAEHRLFSVIFSSYNQYI---RPVENVSDPVIVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLRWDPKDFGGVEFIRVPSNRIWKPDIVLYNNAVGDFQVDDKTKALLRYN--GDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIG--STINLKDFWESGEWMIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRR------VFLGLLPRVMFMTRPDRDPERPQVSGSSARSCTLHNSSSFPQHLTKPQSLPPRTHLLLSTELSTLSEAVKSGSGFLCR--EGRCNCCLRQQPGKIPLDSGVGALGPAGG--------SLGPVSGGLVGNGGGSSPCSSSESLDGGLLAFSALSPEVREAIES-VKYIAENMRLQNEAKEVQDDWKYVAMVIDRIFLWVF 519
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna3 (neuronal acetylcholine receptor subunit alpha-3-like [Source:NCBI gene;Acc:103025148]) HSP 1 Score: 203.756 bits (517), Expect = 4.744e-58 Identity = 122/340 (35.88%), Postives = 212/340 (62.35%), Query Frame = 1
Query: 133 VIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK 1146
++ +IF+ F + +AS E +L + + Y + RPV NV D V+V++ +++ Q++ +DE NQI+ N+W ++ W D L W+PK+F VE +R+P ++IW PDIVLYN A D ++ +K ++ N G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSWTY K+D+ L S I++ D+ ES EW+I+ P + +K D+T+ L ++R ++ +++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R K +P W+R+
Sbjct: 7 ILHYIFICFSA----GGWAS-EAEHRLFSVIFSSYNQYI---RPVENVSDPVIVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLRWDPKDFGGVEFIRVPSNRIWKPDIVLYNNAVGDFQVDDKTKALLRYN--GDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIG--STINLKDFWESGEWMIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRR 334
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:103025036]) HSP 1 Score: 206.838 bits (525), Expect = 9.391e-58 Identity = 124/337 (36.80%), Postives = 202/337 (59.94%), Query Frame = 1
Query: 136 IVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK 1146
+V I + Y+ A E++L+ + Y K + RPV N+ D V+V +GL++ Q++ +DEKNQ++T NVW K W D L WNP+ + NV +R+P + IW PDIVLYN AD + + GRI W PPAI+KS+C ID++ FPFD Q+C +KFGSWTY K+D+ ++ ++D +DY ES EW+I++ + K PD+T+ I++R ++ L++PC+L+S LT+++F+LP E K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R + +P W+R+
Sbjct: 9 LVLIICLQHGYVCTLTPARAHAEERLLQVLFSRYNK---LSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWSDYKLRWNPEEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAQLFHDGRIKWKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDRAKIDLVSMDD--DVDQMDYWESGEWVIINAVGKYNTKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSRTHRMPHWVRQ 340
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrna3 (cholinergic receptor, nicotinic, alpha 3 [Source:ZFIN;Acc:ZDB-GENE-070822-1]) HSP 1 Score: 213.001 bits (541), Expect = 9.158e-62 Identity = 117/296 (39.53%), Postives = 192/296 (64.86%), Query Frame = 1
Query: 259 RPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK 1146
RPV NV D V+V++ +++ Q++ LDE NQI+ N+W K+TW D WNP F +E +R+P DKIW PDIVLYN AD + + Y G ++WMPPAIFKS+C ID++ FPFD+Q+C +KFGSWTY K+D+ L ++I++ D+ ES EW+I+ P + +K PD+T+ + ++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS ++I V+N+++R + +P+W+R+
Sbjct: 24 RPVFNVSDPVIVRFEVSMSQLVKLDEVNQIMATNLWLKHTWNDYKFQWNPGQFEGMEYIRVPSDKIWKPDIVLYNNADGDFQVEGKTKALLKYDGTVMWMPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLVG--TKINLKDFWESGEWVIIDVPGYKHDIKYNCCEQIYPDITYSVYVRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLSISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITIFVLNVHYRTPEAHTMPNWVRR 317
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrnb3b (cholinergic receptor, nicotinic, beta 3b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-030102-4]) HSP 1 Score: 197.208 bits (500), Expect = 2.202e-56 Identity = 125/330 (37.88%), Postives = 199/330 (60.30%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRN-LPDWLRK-GVNATSKILGMKQ 1185
E +L+ +MLKDY+K RPV + DTV VK+GL + Q++ +DEKNQ++T NVW K WVD L WNP ++ + +R+P IW PDIVLY+ AD R V + GRI W PPAI+KS+C ID++ FPFD Q+C +KFGSWTY G +D+ + S++D +Y ++ EW L+ + V + + P +T+ +++R ++ L+VPC+ LS LT+ +F+LP + K+ L ++ V+ ++ L ++ E PS+S +P +G Y MI +TLS +++ VIN++ R + + W+R+ ++ KIL M+
Sbjct: 35 EDRLLKRMLKDYQKWV---RPVDKLSDTVEVKFGLGIAQLVDVDEKNQLMTTNVWLKQEWVDINLRWNPDDYGGINSIRVPASSIWLPDIVLYDNADGRFEGSIVTKAVVKHDGRIKWTPPAIYKSSCTIDVTFFPFDQQNCSMKFGSWTYDGTLVDLIMVN--SDVDRGEYFDNGEWEFLNATGNKGV--RLDGHRFYPYITYSFVIRRLPLFYTLFLIVPCLGLSFLTVFVFYLPSDEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIITVFVINVHHRSSSTHHPMAPWVRQLFLHKLPKILCMRS 357
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrna2a (cholinergic receptor, nicotinic, alpha 2a (neuronal) [Source:ZFIN;Acc:ZDB-GENE-040108-2]) HSP 1 Score: 171.014 bits (432), Expect = 4.215e-46 Identity = 116/329 (35.26%), Postives = 183/329 (55.62%), Query Frame = 1
Query: 292 VKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEKLHLIKS-----EIKNSRNHIK 1260
V+Y + DEKNQ++T NVW K W D L W+P + NV +R+P + IW PDIVLYN AD + + GR+ W PPAI+KS+C ID++ FPFD Q+C +KFGSWTY DK I L ++D+ DY ES EW I+H K PD+T+ I++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y MI +TLS +++ V+N++ R +P W+R+ ++ + L M++ +L L ++ + RNH K
Sbjct: 1 VRYAEFRTHLCDCDEKNQMMTTNVWLKQEWDDYKLQWDPAEYENVTSIRVPSELIWVPDIVLYNNADGDFAVTHMTKAHLFHTGRVRWKPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTY--DKAKIDLVSMARDVDLKDYWESGEWAIVHAVGTYNTKKYDCCPQVYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPATHAMPRWVRRVFLDRVPRWLVMRRPPPQQRRSELLLARAARPPCALGGQRNHHK 327
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: chrnb1l (cholinergic receptor, nicotinic, beta 1 (muscle) like [Source:ZFIN;Acc:ZDB-GENE-060811-1]) HSP 1 Score: 154.066 bits (388), Expect = 1.394e-40 Identity = 121/389 (31.11%), Postives = 212/389 (54.50%), Query Frame = 1
Query: 178 ADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQ--LYENFSEID--IID---YTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRK-GVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISP 1320
A + E+ L+ + + K + RP R V V+ G+ L Q++SL +T++ +W D L W+P+N+ N+ +R+ P ++W PDI L N D V V V+Y G + W+PP+I++S+CG+ + FPFD+Q+C + F S+TY +++++ L E+ EI IID + S EW I HKP+R+ N +D Q D+TF+LI++R ++ +VVPC+L+++ + F+LPP++ KM L +++ +A ++ L LLA+ P S VP + Y M+++T L++IV+NL+FR +P W+R+ +++ K L +++ + KL ++ + H + T S+ + P SP
Sbjct: 16 AGVRGLEAEENLLKDLFVVQQYKTLV-RPARTATGRVTVRLGMTLSQLISLPACQPDMTLSRLISLSWEDPRLSWDPRNYDNLRSIRLSPKRVWLPDIALSNNNDGNFDFGLYVHVLVDYTGAVRWVPPSIYRSSCGMQVKYFPFDWQNCSMVFQSFTYDTSEINLEYALDEHRQEIRHIIIDESTFPVSGEWQIKHKPSRKNT--NPRDPLYQ-DITFYLIMERKPLFYIINIVVPCILITICAVFTFYLPPDAGEKMGLCISVLLALTVFLLLLADKVPETSLGVPVIVNYLMFTMVIVTACVILTVIVLNLHFRSPATHIMPYWVRRIFIHSLPKYLWIERPKKEELAPKLPVLTQH----QQHCHDEYFIRTPPSQALLLLPTSP 396
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008550.1 (pep scaffold:Pmarinus_7.0:GL476601:34:38373:1 gene:ENSPMAG00000007717.1 transcript:ENSPMAT00000008550.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 146.362 bits (368), Expect = 1.849e-38 Identity = 93/241 (38.59%), Postives = 142/241 (58.92%), Query Frame = 1
Query: 421 NVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
V+ +R P +IWTPDI+LYN ADER V VNY G +PP + KSTC ID+ FPFD Q C LKFGSWT+ G +D+Q+ E D Y + EW ++ P +R L PD+T+ L ++R + Y+ L+VPCVL+S L+L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP +G Y+ MI++ S +++IV+ + + +P W+R
Sbjct: 5 GVKNLRFQPHQIWTPDILLYNSADERFDATFHTNVLVNYTGNCQHLPPGMLKSTCYIDVRWFPFDVQKCDLKFGSWTHDGWLIDLQMLEA----DTSGYIANGEWDLVEIPGKRNELYYDCCQEPYPDVTYTLTIRRRTLYYGLNLLVPCVLISALSLLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIGQYFASTMIIVAFSVVVTVIVLQYHHHNPDGKKMPQWVR 241
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO32052 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW62]) HSP 1 Score: 191.045 bits (484), Expect = 9.125e-56 Identity = 108/268 (40.30%), Postives = 165/268 (61.57%), Query Frame = 1
Query: 256 GRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYAD---ERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNM 1050
RPV +V+D VVVK GL L QI+ +DEKNQILT NVW + W D +L WNP++F V+ + + P+ +W PDI+LYN D E L +K D V ++ G W+ PAI K+ C +D+ FPFD Q C LKFGSWT+ G K+DIQL + + +DI DY+E+ EW + P RR V P +TF L+++R + ++ Y +V+P +++LL+L F+LPP S ++ + + ++ S+ + ++ E P S VP + ++ +M
Sbjct: 9 ARPVLHVKDPVVVKMGLMLKQIIDVDEKNQILTTNVWIRLYWKDHLLNWNPEDFGGVKSINVEPNNVWKPDILLYNPTDLRVESLYKKIDTKVILSSDGSAAWLSPAIIKTECKVDVRLFPFDVQYCDLKFGSWTHDGLKIDIQLRKT-TGVDIDDYSENREWFLKDAPGRRDVKLYNCCPEPYPTVTFTLVIRRRAMFYVYNMVLPTGMIALLSLFSFYLPPNSGERVSFVITVLLSMSVYMIMVTENMPQ-SADVPLVSKFFMASM 274
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO42054 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2J7]) HSP 1 Score: 195.282 bits (495), Expect = 2.115e-55 Identity = 119/322 (36.96%), Postives = 190/322 (59.01%), Query Frame = 1
Query: 196 SGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERL---IEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQ-P--DLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLR 1143
+ EK+L+ + ++Y + RPV N D V V++ L+LIQI++++ K+Q++TV+VW + W + ML WN + +E V + P K+W PDI+LYN ADE +EK V + G W PA F STC I++ FPFD Q+C LKFGSWTY G++L + + D+ Y + EW +L PA+ L + N + P D+T +L ++R Y+ + L++PC L++ LTL+ F+LPPES K+ LG+ + +A ++ L L+AET PS S +P LG Y+ M + +S + +++N + R +P W++
Sbjct: 36 TAEKRLLADLFRNYDSEV---RPVFNDSDKVNVEFSLSLIQIIAVNSKSQLVTVSVWIRQKWTNPMLAWNTSKYGGIESVNLNPAKVWIPDIILYNNADETYAGGLEKYKTSVIASSDGTQEWNSPASFTSTCNINVRYFPFDSQNCSLKFGSWTYVGNRLTMTSTSD--SADLSKYVNNGEWHLLAMPAK---LNRVFYNCCKYPFYDVTLYLEIQRKPLYYVFNLIIPCALIATLTLIKFFLPPESGEKVGLGITVLLAMTVFLLLVAETLPSTSDHIPLLGQYFVATMFVTAISLVATCLILNFFHRNPSTSPMPHWVK 349
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO32053 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW63]) HSP 1 Score: 190.274 bits (482), Expect = 5.657e-55 Identity = 108/286 (37.76%), Postives = 172/286 (60.14%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADER---LIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNM 1050
E L+ +L++Y K RPV NV D V +K G+ L Q++ L+E++QILT+NVW + W D +L WNP +F ++ + + PD++W PD++LYN D R L K D V VN+ G W+ PAI KS C ID+ FPFD QSC L FGSWT+ G K+DI L + S ID +++++ EW + RR V + P +T+ +IL+R + ++ + +V+P +++LL+L F+LPP S ++ + + +A S+ L ++ E P ST +P + ++ +M
Sbjct: 1 EPALLKYLLQNYSKDA---RPVLNVNDPVQLKLGIMLRQVIDLNERDQILTINVWIRQYWKDHLLKWNPDDFGGIKSINIAPDRVWKPDVLLYNRTDLRFGSLNNKIDTNVIVNHDGSTTWLAPAIIKSECKIDVRFFPFDVQSCELTFGSWTHDGLKIDIHLKQE-SGIDTENFSDNREWFLKGTSGRRDVKMYICCPEPYPTVTYTIILRRRAMFYVFNMVLPTGVIALLSLFSFYLPPNSGERVSFVITVLLAMSVYLIMVTENIPR-STDIPLVSKFFMASM 281
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO47969 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKU6]) HSP 1 Score: 182.185 bits (461), Expect = 6.696e-52 Identity = 108/307 (35.18%), Postives = 180/307 (58.63%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLI---EKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQP--DLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFR 1107
E++LI+ +L+ Y + RPV N T+ V +G+ ++QI++++ +NQI+ NVW + W +E+L W+P N+ V+ VR+ +W PDIVLY+ AD EK V ++ G W PA F+STC ID++ FPFD Q+CY+KFGSWTY +DIQ + + + Y +S EW ++ R + +P D+T + ++R ++A+ L+ PC+++ + L+ F+LPPES ++ L + + +A ++ L+L+AET P S S+P LG +Y M I +S + V+N++ R
Sbjct: 1 EQRLISDLLQGYSRNA---RPVLNSNHTLKVFFGMEIVQIVNVENRNQIIQTNVWIRQRWDNELLTWHPSNYEGVQTVRLDAKSVWIPDIVLYDSADSVFSGGHEKYKTHVIISKDGSSAWFSPASFRSTCKIDVTYFPFDEQTCYMKFGSWTYTIADVDIQ--ASPAPVKSSKYLKSAEWDLMSATTSR--KSQIYACCPEPISDVTIAMTIRRKPLFYAFNLITPCMIMLTMILLGFFLPPESGERITLSITVLLAMAVFLQLVAETLPRNSESIPLLGRFYITIMTEIAVSLMATCWVLNIHHR 300
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Match: EDO43642 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY59]) HSP 1 Score: 172.17 bits (435), Expect = 1.019e-48 Identity = 103/286 (36.01%), Postives = 167/286 (58.39%), Query Frame = 1
Query: 202 EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYAD---ERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNM 1050
E KL+ + +Y RPV +V V+ G++L QI+ LDE+NQIL N+W + W + +L W+P +F N+ + + P ++W PD++LYN AD + +++ V + + G +W+ P IFKSTC D++ FPFD QSC LKFGSWTY G +L+I + EN ++I I + + EW + A R V+ PD+TF L ++R + ++ +++PC L++ LTL+ + LPPES ++ L + +A ++ L L++E P + VP + YY M
Sbjct: 2 ENKLLKDLFSNYNPSV---RPVLKATSSVTVQLGVSLHQIIDLDERNQILISNLWVRQKWNNPLLTWDPTHFGNLTSINVDPLRVWRPDLILYNNADRDTDGALDRFYTKVQLKFDGSSLWLAPIIFKSTCKFDVAYFPFDEQSCILKFGSWTYDGLRLNI-VPENGTDIGIDKFVSNGEWELTGVSANRNVMFYDCCVEPYPDVTFTLKIRRLTLFYYMNMLIPCFLITGLTLLSYILPPESGERITLVVTSLLAQAVYLLLVSEIMPPVAEVVPLISIYYTTAM 283
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: si:ch73-380n15.2 (neuronal acetylcholine receptor subunit alpha-7 [Source:NCBI gene;Acc:101162456]) HSP 1 Score: 213.772 bits (543), Expect = 6.016e-61 Identity = 132/356 (37.08%), Postives = 206/356 (57.87%), Query Frame = 1
Query: 127 IGVIVWIFVIFPSYITLADYASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQ 1185
+G+ +W +F SM GE +KL ++L +Y + + RPV N ++V+ G L+QI+ +DEKNQ+L N W + W D L WNP+N+ V+ +R P D++WTPDI+LYN ADER V VN G ++PP I KSTC ID+ FPFD Q C LKFGSWT+ G LD+Q+ ++DI Y + EW ++ PA+R L PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ M+++ +S +++IV+ + +P W+R +N + L MKQ
Sbjct: 12 VGLYIWAAFLFK--------GSMQGEYQRKLYKELLANYNR---LERPVVNDSAAILVELGFTLLQIIDVDEKNQVLITNAWLQLHWTDIYLTWNPENYPGVQNLRFPSDQVWTPDILLYNSADERFDATFHTNVLVNASGHCQYIPPGILKSTCYIDVRWFPFDVQKCDLKFGSWTHNGWLLDLQML----DVDISTYISNGEWDLVGVPAKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGMSVVVTVIVLQFHHHDPHGGKMPKWVRVVLLNWCAWFLRMKQ 352
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: si:ch73-380n15.2 (neuronal acetylcholine receptor subunit alpha-7 [Source:NCBI gene;Acc:101162456]) HSP 1 Score: 212.231 bits (539), Expect = 2.905e-60 Identity = 129/336 (38.39%), Postives = 199/336 (59.23%), Query Frame = 1
Query: 187 ASMSGE--KKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQ 1185
SM GE +KL ++L +Y + + RPV N ++V+ G L+QI+ +DEKNQ+L N W + W D L WNP+N+ V+ +R P D++WTPDI+LYN ADER V VN G ++PP I KSTC ID+ FPFD Q C LKFGSWT+ G LD+Q+ ++DI Y + EW ++ PA+R L PD+TF + ++R + Y+ L++PCVL+S L L++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP + Y+ M+++ +S +++IV+ + +P W+R +N + L MKQ
Sbjct: 48 GSMQGEYQRKLYKELLANYNR---LERPVVNDSAAILVELGFTLLQIIDVDEKNQVLITNAWLQLHWTDIYLTWNPENYPGVQNLRFPSDQVWTPDILLYNSADERFDATFHTNVLVNASGHCQYIPPGILKSTCYIDVRWFPFDVQKCDLKFGSWTHNGWLLDLQML----DVDISTYISNGEWDLVGVPAKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGMSVVVTVIVLQFHHHDPHGGKMPKWVRVVLLNWCAWFLRMKQ 376
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: chrna4b (neuronal acetylcholine receptor subunit alpha-2 [Source:NCBI gene;Acc:101158195]) HSP 1 Score: 210.69 bits (535), Expect = 6.357e-59 Identity = 137/386 (35.49%), Postives = 226/386 (58.55%), Query Frame = 1
Query: 199 GEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEKLHLIK--------SEIKNSRNHIKR--QQEKETTESRETIPTPISPR 1323
E++L+ + Y K + RPV+N DTV+V +GL++ Q++ +DEKNQ++T NVW K W D L WNP+++ NV +R+P + IW PDIVLYN AD + Y G+I WMPPAI+KS+C ID++ FPFD QSC +KFGSWTY D+ I L S++D +DY ES EW+I++ + K D+T++ I++R ++ L++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L L+ E PS S +P +G Y M+ +TLS ++I V+N++ R + +P W+R+ ++ ++L MK+ +A LI+ S NS++ + ++ T+ ++P SPR
Sbjct: 44 AEERLLQNLFARYNK---LSRPVQNTSDTVLVHFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWNPEDYENVTSIRIPSEIIWRPDIVLYNNADGDFAVTHLTKAHLFYDGQIKWMPPAIYKSSCSIDVTFFPFDQQSCKMKFGSWTY--DRAKIDLISMASDVDQMDYWESGEWVIVNAVGKYNTKKYECCAEIYADITYYFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSQCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMVFVTLSIIITIFVLNVHHRSPQTHGMPHWVRRVFLDLVPRVLFMKRPPGTAKQHCKRLIEMMHCPTTISATGNSQSFWTGLDSELRQMTQVHNSLPKVDSPR 424
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: chrna3 (neuronal acetylcholine receptor subunit alpha-3 [Source:NCBI gene;Acc:101159658]) HSP 1 Score: 206.068 bits (523), Expect = 6.179e-58 Identity = 133/413 (32.20%), Postives = 237/413 (57.38%), Query Frame = 1
Query: 175 LADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYA--DERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGM-------KQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSS 1383
L+ E KL + + +Y + RPV NV D V+V++ +++ Q++ +DE NQI+ N+W ++ W D L WNPK F VE +R+P ++IW PDIVLYN A D ++ +K ++ N G + W+PPAIFKS+C ID++ FPFD+Q+C +KFGSWTY K+D+ L S I++ D+ ES EW I+ P + +K D+T+ L ++R ++ +++PC+L+S LT+++F+LP + K+ L +++ ++ ++ L ++ ET PS S +P +G Y MI +TLS +++ V+N+++R K +P W+R+ + +++ M ++V +S + +HL + +S Q+ + ++ + ++ R R ++ + N SG S+
Sbjct: 19 LSGGTCSEAEHKLFSVIFSNYNQYI---RPVENVSDPVIVQFEVSMSQLVKVDEVNQIMETNLWLRHIWNDYKLKWNPKEFGGVEFIRVPSNRIWKPDIVLYNNAVGDFQVDDKTKALLRFN--GDVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDLVLIG--STINLKDFWESGEWTIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKTHTMPCWVRRVFLGLLPRVMFMTRPERDPEKVAVGSSVQTVHLRPCTVHSSGT----LQKHKARAPGSSMMSSLTNRQRILNNTELSNLNNRSGDSN 420
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Match: si:ch211-39a7.1 (neuronal acetylcholine receptor subunit alpha-7-like [Source:NCBI gene;Acc:101160921]) HSP 1 Score: 204.912 bits (520), Expect = 2.076e-57 Identity = 166/539 (30.80%), Postives = 260/539 (48.24%), Query Frame = 1
Query: 184 YASMSG--EKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQP--DLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKG-VNATSKILGMKQVISSASSEK----LHLIK-------SEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSK-------MAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRL 1731
+ S+ G ++ L+ +LKDY + + RPV N T+ V + L+LIQIM +DEKNQ+LT N+W + TW D L WN + V+ +R D++WTPDI+LYN AD+ V VN G ++PP IF STC +D+ FPFD Q C LKFGSWTY G LD+Q+ E DI Y + EW ++ P R + D +P D+TF + ++R + Y+A L++PCVLLS +TL++F LP +S K+ LG+ + ++ ++ + L+AE P+ S SVP +G Y+ MI++ +S + +V+ + N+P W++ + + L MK+ S E+ HL + I N +H ++ +T P + H S + M Q++ SS P Q P A S P P A A P S+ +A + + L I + ++ + + E+D +A +W+ V+DRL
Sbjct: 17 HVSLQGPHQRSLLKSLLKDYNR---MERPVANDSHTLTVHFTLSLIQIMDVDEKNQVLTSNMWLQMTWYDHYLQWNQSEYPGVKNLRFTSDQVWTPDILLYNSADDDFDSTFKTNVLVNSSGYAKYLPPGIFMSTCNVDVRWFPFDIQKCNLKFGSWTYDGWLLDLQMI----EADISSYMPNGEWDLIGVPGSRNEI--FYDCCKEPYTDVTFVITIRRRTLYYALNLLIPCVLLSSMTLLVFVLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIGQYFASIMIIVGMSVIATTVVLQYHHHDPNGGNMPKWVQLVLLQWVAWFLRMKRPGESDDPERPPCAPHLRRCSSGSQSGSIPNPPDHTLHPLHPQSLAPLQTGPLHVGHPHLHTQSTTNNNGNLLYMGFQSIEDSSV-----LSEPVQRNVSAGPPRVAGSPPPH---LPSQFCSSPPPPASNMDTVACPSTVSSGGGFGGGVGGCSTAGIGDPQLQAILEEVRYMADRFREQDEAESVANQWKFAGAVIDRL 538
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000069967.1 (SMESG000069967.1) HSP 1 Score: 1005.74 bits (2599), Expect = 0.000e+0 Identity = 576/577 (99.83%), Postives = 576/577 (99.83%), Query Frame = 1
Query: 118 MRLIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYEK 1848
MRLIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSST KKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK
Sbjct: 1 MRLIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSSTTKKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK 577
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000069967.1 (SMESG000069967.1) HSP 1 Score: 968.763 bits (2503), Expect = 0.000e+0 Identity = 557/568 (98.06%), Postives = 560/568 (98.59%), Query Frame = 1
Query: 145 IFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYEK 1848
I ++F + DYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSST KKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK
Sbjct: 3 ILIVFMILLLGVDYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSSTTKKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK 570
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000069967.1 (SMESG000069967.1) HSP 1 Score: 966.066 bits (2496), Expect = 0.000e+0 Identity = 555/557 (99.64%), Postives = 555/557 (99.64%), Query Frame = 1
Query: 178 ADYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYEK 1848
DYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSST KKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK
Sbjct: 7 VDYASMSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSSTTKKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK 563
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000069967.1 (SMESG000069967.1) HSP 1 Score: 956.822 bits (2472), Expect = 0.000e+0 Identity = 551/552 (99.82%), Postives = 551/552 (99.82%), Query Frame = 1
Query: 193 MSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYEK 1848
MSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSST KKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK
Sbjct: 1 MSGEKKLITKMLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSSTTKKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK 552
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Match: SMESG000069967.1 (SMESG000069967.1) HSP 1 Score: 937.562 bits (2422), Expect = 0.000e+0 Identity = 541/542 (99.82%), Postives = 541/542 (99.82%), Query Frame = 1
Query: 223 MLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYXXXXXXXXXXXXXXXXXXXPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMXXXXXXXXXXXXXXNLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVXXXXXXXXXXXXXXKSMDQILEEYARKYEK 1848
MLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSST KKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK
Sbjct: 1 MLKDYKKKGKIGRPVRNVQDTVVVKYGLALIQIMSLDEKNQILTVNVWAKYTWVDEMLIWNPKNFSNVEEVRMPPDKIWTPDIVLYNYADERLIEKRDVMVSVNYIGRIIWMPPAIFKSTCGIDISNFPFDFQSCYLKFGSWTYKGDKLDIQLYENFSEIDIIDYTESNEWLILHKPARRFVLKNMKDNTSQPDLTFFLILKRNSAYHAYLLVVPCVLLSLLTLVIFWLPPESPAKMILGMNIFVAFSLLLRLLAETTPSASTSVPFLGYYYCLNMILITLSTFLSIIVINLYFRCDKRRNLPDWLRKGVNATSKILGMKQVISSASSEKLHLIKSEIKNSRNHIKRQQEKETTESRETIPTPISPRARHKSSKMAMEMQNLSGSSSNYQTRFQYPRQNTDVTDIPYTAHSDIPRNSFNPGYSYEYRYSNAPPPAAPASPLPSSTTKKQTQQKKSNQSAILLEKNLSEIRKALKNLMTKIAEKDRGNRIAKEWRMVAMVLDRLFFWVYFVIVILSGLILLLPRGLPKSMDQILEEYARKYEK 542 The following BLAST results are available for this feature:
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Neuronal acetylcholine receptor subunit alpha-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30031159 ID=SMED30031159|Name=Neuronal acetylcholine receptor subunit alpha-4|organism=Schmidtea mediterranea sexual|type=transcript|length=2010bpback to top protein sequence of SMED30031159-orf-1 >SMED30031159-orf-1 ID=SMED30031159-orf-1|Name=SMED30031159-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=578bp MRLIGVIVWIFVIFPSYITLADYASMSGEKKLITKMLKDYKKKGKIGRPVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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