Rho GTPase-activating protein 20

Overview
NameRho GTPase-activating protein 20
Smed IDSMED30031095
Length (bp)2407
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rho GTPase-activating protein 20 (SMED30031095) t-SNE clustered cells

Violin plots show distribution of expression levels for Rho GTPase-activating protein 20 (SMED30031095) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rho GTPase-activating protein 20 (SMED30031095) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rho GTPase-activating protein 20 (SMED30031095) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031095

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30031095h1SMcG0012610 SmedASXL_013412SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30031095h1SMcG0012610 SmedASXL_013412SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30031095h1SMcG0012610 Contig19319uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031095h1SMcG0012610 Contig19319newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031095h1SMcG0012609 Contig19319uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031095h1SMcG0012609 Contig19319newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parapharyngeal regionSMED30031095h1SMcG0012610 dd_Smed_v6_21560_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30031095h1SMcG0012610 dd_Smed_v4_21560_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Celegans
Match: rga-2 (Rho GTPase Activating protein [Source:UniProtKB/TrEMBL;Acc:Q9XW53])

HSP 1 Score: 53.9138 bits (128), Expect = 4.500e-7
Identity = 48/157 (30.57%), Postives = 81/157 (51.59%), Query Frame = 1 
Query:  919 PKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCN-DFIISEDIFNK------AAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFM-EISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIF 1365
            P+ +  ++  +  DG D++G+FR + K S+F +     L C  D  +  D F+K      A+I K +LR+I G ++       L+G  + +M EI+ E      KK S    L   LP +H  L  N+L +LN I +SP++K+   +++  +APS  
Sbjct:  264 PQPIMTIVDHLRMDGFDAEGIFRKSPKQSTFKE-----LKCELDKGVVPD-FHKYNTHVLASILKEYLRSIPGKIL-------LSGNYELWMREIADEP--NTEKKVSCCRALLSHLPTSHSILLANVLKLLNKISNSPSSKMNASSLSVCLAPSFL 405
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Xenopus
Match: trhd (tumorhead [Source:Xenbase;Acc:XB-GENE-1214876])

HSP 1 Score: 65.855 bits (159), Expect = 2.910e-10
Identity = 44/156 (28.21%), Postives = 83/156 (53.21%), Query Frame = 1 
Query:  910 GEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFII-SEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAI-IDSPNAKVEPFAIARAIAPSIFPL 1371
            G +PK +  +L  +Y +GP++KG+FR +A   ++ +  + ++  ++  I  E +F  AA+   FLRNI  S+++ +    + GL  +  EI   E+     K     +L + LP+ ++ L Q++  VL+ I   S   ++  F +A  IAP++  L
Sbjct:  365 GNLPKPIMDMLLLLYEEGPNTKGIFRRSANAKTWKELKEKLISGDEVQIDGESVFVAAAVITDFLRNIPDSILSSD----MYGLWMEATEIEQHEY-----KIDIIKRLLEQLPEANFILLQHLFGVLHHIEKKSEENQMTAFNLALCIAPTMLWL 511
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Xenopus
Match: gapvd1 (GTPase activating protein and VPS9 domains 1 [Source:Xenbase;Acc:XB-GENE-997388])

HSP 1 Score: 53.5286 bits (127), Expect = 1.622e-6
Identity = 46/153 (30.07%), Postives = 77/153 (50.33%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLD--CNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIID-SPNAKVEPFAIARAIAPSIF 1365
            +PK +  +L  +  DGP ++G+FR +A   S  +  K  LD  C      E I   A++FK FLRNI GS+ +       + L +K++ +   E  ++  K  K   L ++LP+ +  L + +  VL  I   S + ++  F +A  IAPS+ 
Sbjct:  376 IPKPVMDMLCVLCRDGPFTRGIFRRSANAKS-CREMKERLDSGCEINFERESILVTASVFKDFLRNIPGSIFS-------SKLYEKWVSVLAYESQEDKIKAVKM--LIETLPQINVLLLRYLFGVLYCIEQRSESNQMSAFNLAVCIAPSLL 518
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Xenopus
Match: gapvd1 (GTPase activating protein and VPS9 domains 1 [Source:Xenbase;Acc:XB-GENE-997388])

HSP 1 Score: 52.7582 bits (125), Expect = 2.639e-6
Identity = 46/153 (30.07%), Postives = 76/153 (49.67%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLD--CNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIID-SPNAKVEPFAIARAIAPSIF 1365
            +PK +  +L  +  DGP ++G+FR +A   S  +  K  LD  C      E I   A++FK FLRNI GS+ +         L +K++ +   E  ++  K  K   L ++LP+ +  L + +  VL  I   S + ++  F +A  IAPS+ 
Sbjct:  316 IPKPVMDMLCVLCRDGPFTRGIFRRSANAKS-CREMKERLDSGCEINFERESILVTASVFKDFLRNIPGSIFSSK-------LYEKWVSVLAYESQEDKIKAVKM--LIETLPQINVLLLRYLFGVLYCIEQRSESNQMSAFNLAVCIAPSLL 458
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Xenopus
Match: gapvd1 (GTPase activating protein and VPS9 domains 1 [Source:Xenbase;Acc:XB-GENE-997388])

HSP 1 Score: 52.7582 bits (125), Expect = 2.901e-6
Identity = 46/153 (30.07%), Postives = 77/153 (50.33%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLD--CNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIID-SPNAKVEPFAIARAIAPSIF 1365
            +PK +  +L  +  DGP ++G+FR +A   S  +  K  LD  C      E I   A++FK FLRNI GS+ +       + L +K++ +   E  ++  K  K   L ++LP+ +  L + +  VL  I   S + ++  F +A  IAPS+ 
Sbjct:  316 IPKPVMDMLCVLCRDGPFTRGIFRRSANAKS-CREMKERLDSGCEINFERESILVTASVFKDFLRNIPGSIFS-------SKLYEKWVSVLAYESQEDKIKAVKM--LIETLPQINVLLLRYLFGVLYCIEQRSESNQMSAFNLAVCIAPSLL 458
[Close]
BLAST of Rho GTPase-activating protein 20 vs. TrEMBL
Match: H0ZLY7 (Rho-GAP domain-containing protein OS=Taeniopygia guttata OX=59729 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 5.240e-10
Identity = 51/153 (33.33%), Postives = 80/153 (52.29%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFI--ISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAI-IDSPNAKVEPFAIARAIAPSIF 1365
            +PK L  +LS +Y +GP +KG+FR +    +F K  K  LD    +    E IF  A++FK FLRNI GS+++         LC K+  +S  +   N +K     +L + LP+ +  L + I  VL+ I + S   ++  F +A  IAPS+ 
Sbjct:   17 LPKPLLDMLSLLYQEGPSTKGIFRRSGNAKTF-KELKEKLDSGTEVDLARESIFVTASLFKDFLRNIPGSILSSQ-------LCDKW--VSVLDQGNNEEKIKSIQRLIEHLPRANVVLLRYIFGVLHGIEMRSEENQMNAFNLAVCIAPSLL 159
[Close]
BLAST of Rho GTPase-activating protein 20 vs. TrEMBL
Match: U3K292 (Rho GTPase activating protein 20 OS=Ficedula albicollis OX=59894 GN=ARHGAP20 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.040e-9
Identity = 74/261 (28.35%), Postives = 122/261 (46.74%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFI--ISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAI-IDSPNAKVEPFAIARAIAPSIF-------PLVRLSYWGNISNLLEAAI------------YLTDKIVKPADRKRIID---------SYIKVKNESNNIKNESRNDSQTFVENDSMTLSSI---TESDSKQ 1596
            +PK L  +LS +Y +GP +KG+FR +    +F K  K  LD    +    E IF  A++FK FLRNI GS+++         LC K+  IS  +   N +K     +L + LP+ +  L + I  VL+ I + S   ++  F +A  IAPS+        P +   Y   IS+L++  I             L  +I+    R+   D         SY  ++NE+N+  + S +D     + D+ +  S+   ++ DS+Q
Sbjct:  376 LPKPLLDMLSLLYQEGPSTKGIFRRSGSAKTF-KELKEKLDSGTEVDLARESIFVTASLFKDFLRNIPGSILSSQ-------LCDKW--ISVLDQGNNEEKIKSIQRLIEHLPRANVVLLRYIFGVLHGIEMRSEENQMNAFNLAVCIAPSLLWPPVSSTPDIESEYIKKISSLVQFLIENCCRIFGDEITSLFGEILMTCKRENSSDVASLHQNDSSYDSLENEANDEADSSSSDWIKTRDQDNRSRESVFTLSDGDSEQ 626
[Close]
BLAST of Rho GTPase-activating protein 20 vs. TrEMBL
Match: A0A218UTQ4 (Rho GTPase-activating protein 20 OS=Lonchura striata domestica OX=299123 GN=ARHGAP20_0 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.401e-9
Identity = 51/153 (33.33%), Postives = 80/153 (52.29%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFI--ISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAI-IDSPNAKVEPFAIARAIAPSIF 1365
            +PK L  +LS +Y +GP +KG+FR +    +F K  K  LD    +    E IF  A++FK FLRNI GS+++         LC K+  +S  +   N +K     +L + LP+ +  L + I  VL+ I + S   ++  F +A  IAPS+ 
Sbjct:  375 LPKPLLDMLSLLYQEGPSTKGIFRRSGSAKTF-KELKEKLDLGTEVDLARESIFVTASLFKDFLRNIPGSILSSQ-------LCDKW--VSVLDQGNNEEKIKSIQRLIEHLPRANVVLLRYIFGVLHGIEMRSEENQMNAFNLAVCIAPSLL 517
[Close]
BLAST of Rho GTPase-activating protein 20 vs. TrEMBL
Match: A0A093J0F8 (Rho GTPase-activating protein 20 (Fragment) OS=Eurypyga helias OX=54383 GN=N326_11208 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 7.553e-9
Identity = 49/153 (32.03%), Postives = 79/153 (51.63%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFI--ISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAI-IDSPNAKVEPFAIARAIAPSIF 1365
            +PK L  +LS +Y +GP ++G+FR  +  +   K  K  LD    +    E IF  A++FK FLRNI GS+++         LC K+  +S  +   N +K     +L + LP+ +  L + I  VL+ I + S   ++  F +A  IAPS+ 
Sbjct:   17 LPKPLLDMLSLLYQEGPSTEGIFR-RSGSAKTCKELKEKLDSGAEVDLACESIFVTASLFKDFLRNIPGSILSSQ-------LCDKW--VSVMDQGNNEEKIKSIQRLIEQLPRANVVLLRYIFGVLHGIEMRSEENQMNAFNLAICIAPSLL 159
[Close]
BLAST of Rho GTPase-activating protein 20 vs. TrEMBL
Match: A0A2P4T5M9 (Uncharacterized protein (Fragment) OS=Bambusicola thoracicus OX=9083 GN=CIB84_004620 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.084e-8
Identity = 57/187 (30.48%), Postives = 93/187 (49.73%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFI--ISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAI-IDSPNAKVEPFAIARAIAPSIF-------PLVRLSYWGNISNLLEAAIYLTDKIVK 1446
            +PK L  +LS +Y +GP ++G+FR  +  +   K  K  LD    +    E IF  A++FK FLRNI GS++T         LC K+  +S  +   N +K     +L + LP+ +  L + I  VL+ I + S   ++  F +A  IAPS+        P +   +   IS+L++   +LT+   K
Sbjct:  161 LPKPLLDMLSLLYQEGPSTEGIFR-RSGSAKTCKELKEKLDSGAEVDLACESIFVTASLFKDFLRNIPGSILTSQ-------LCDKW--VSVMDQGNNEEKIKSIQRLIEQLPRANVVLLRYIFGVLHGIEMRSEENQMNAFNLAICIAPSLLWPPASSTPDIESEFTKKISSLVQ---FLTENCCK 334
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Cavefish
Match: ENSAMXT00000053633.1 (rho GTPase-activating protein 20-like [Source:NCBI gene;Acc:103036999])

HSP 1 Score: 52.7582 bits (125), Expect = 1.813e-6
Identity = 44/151 (29.14%), Postives = 76/151 (50.33%), Query Frame = 1 
Query:  916 MPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCN--DFIIS-EDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVL-NAIIDSPNAKVEPFAIARAIAP 1356
            +PK +  +L  +Y +GP ++G+FR +A  +   +  + +LD    D  ++ + IF  AA+FK FLR+I GSL+       L+ L +K+M            +      L  SLP  +  L ++IL VL N  + +   ++  F +A  I+P
Sbjct:  375 LPKPIMDMLVFLYQEGPFTRGIFRRSA-GAKACRELRDILDSGFQDIQLARQSIFVIAAVFKDFLRSIPGSLL-------LSDLYEKWMATMDSSDEAEDNQLQAIQSLVTSLPSENILLLKHILAVLYNIQLRAEENQMNAFNLAVCISP 517
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Planmine SMEST
Match: SMESG000074055.1 (SMESG000074055.1)

HSP 1 Score: 1408.66 bits (3645), Expect = 0.000e+0
Identity = 743/749 (99.20%), Postives = 747/749 (99.73%), Query Frame = 1 
Query:  130 MRMNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVXXXXXXXXXXXXXXDLCMKHALISRATDVATPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADRKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNLISCDKLSDCELEKESDIENFSQDSILXXXXXXXXXXXXXXXXXCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIESDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDXXXXXXXXXXXXEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ 2376
            MRMNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVKLYLIKKIEEKLILDLCMKHALISRATDVA PKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIY DGPDSKGVFRLNAKDSSFSKWYKHMLDC+DFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPAD+KRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQN+ISCDKLSDCELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIE+DHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISSSSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ
Sbjct:    1 MRMNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVKLYLIKKIEEKLILDLCMKHALISRATDVAIPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYCDGPDSKGVFRLNAKDSSFSKWYKHMLDCDDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADKKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNMISCDKLSDCELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIENDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISSSSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ 749
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Planmine SMEST
Match: SMESG000074055.1 (SMESG000074055.1)

HSP 1 Score: 1403.27 bits (3631), Expect = 0.000e+0
Identity = 741/747 (99.20%), Postives = 745/747 (99.73%), Query Frame = 1 
Query:  136 MNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVXXXXXXXXXXXXXXDLCMKHALISRATDVATPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADRKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNLISCDKLSDCELEKESDIENFSQDSILXXXXXXXXXXXXXXXXXCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIESDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDXXXXXXXXXXXXEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ 2376
            MNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVKLYLIKKIEEKLILDLCMKHALISRATDVA PKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIY DGPDSKGVFRLNAKDSSFSKWYKHMLDC+DFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPAD+KRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQN+ISCDKLSDCELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIE+DHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISSSSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ
Sbjct:    1 MNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVKLYLIKKIEEKLILDLCMKHALISRATDVAIPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYCDGPDSKGVFRLNAKDSSFSKWYKHMLDCDDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADKKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNMISCDKLSDCELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIENDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISSSSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ 747
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Planmine SMEST
Match: SMESG000074055.1 (SMESG000074055.1)

HSP 1 Score: 1378.62 bits (3567), Expect = 0.000e+0
Identity = 728/734 (99.18%), Postives = 732/734 (99.73%), Query Frame = 1 
Query:  175 MDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVXXXXXXXXXXXXXXDLCMKHALISRATDVATPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADRKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNLISCDKLSDCELEKESDIENFSQDSILXXXXXXXXXXXXXXXXXCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIESDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDXXXXXXXXXXXXEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ 2376
            MDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVKLYLIKKIEEKLILDLCMKHALISRATDVA PKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIY DGPDSKGVFRLNAKDSSFSKWYKHMLDC+DFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPAD+KRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQN+ISCDKLSDCELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIE+DHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISSSSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ
Sbjct:    1 MDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLSKDRVKLYLIKKIEEKLILDLCMKHALISRATDVAIPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYCDGPDSKGVFRLNAKDSSFSKWYKHMLDCDDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADKKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNMISCDKLSDCELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQTSSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTESEFQIENDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQSDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISSSSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ 734
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Planmine SMEST
Match: SMESG000040021.1 (SMESG000040021.1)

HSP 1 Score: 167.548 bits (423), Expect = 2.643e-44
Identity = 109/384 (28.39%), Postives = 196/384 (51.04%), Query Frame = 1 
Query:  340 CMKHALISRATDVATP-KIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNH-TIEYENGQIPYSIEEKWGVGEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFIISEDIFNKAAIFKFFLRNIEGSLMTKN-TRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEPFAIARAIAPSIFPLVRLSYWGNIS-NLLEAAIYLTDKIVKPADRKRIIDSY 1479
            C+KH  ++  +D + P   +   L+ DP   + +++  +P++     + F +    +N + + I F + + N +    K S  ++++G   G DL++ I+            Y+L L+ G+  + +RDD ++L ++EH  +   K  C  GC+ ES  P+  L + I   N   IE++     YS++EKW   E+P+WL +LL+D+Y +GP+++G+FR +A       W   +     F I++    KA+I K F+R I+  L +K    K L+     F  ++ EEF  +  K    IK   +LPK ++   QN+LVVL AII++       + I+     S F      +    +  L E  I LTD++V P+D+++II+++
Sbjct:   63 CLKHTYVTFVSDESIPISKNKSLLLGDPLRNDFIVDIGNPNLMLSCHTNFFKERERFNKELSRIKFPILIKNTIATFDKMSVRITVNGSISGTDLRNHIDANLNLN--KGTQYLLLLNTGKTFVPLRDDVSLLMLYEHFTFCEKKYICAQGCYIESKAPDKELEVYIKLDNRKLIEFKIND--YSLKEKWINDEIPRWLMKLLADLYFEGPETRGIFRESASVQRIRSWINQIDSKKYFTIADKPLVKASILKIFIRGIKQGLFSKGYIGKHLDIFY--FQRLTVEEFLSDENKTDDLIKDLMNLPKKNFCFLQNLLVVLGAIINNNTNNNTVYNISVLFNQSFFDNENELFKQEATLKLTETIIKLTDRLVGPSDKQKIIENF 440
[Close]
BLAST of Rho GTPase-activating protein 20 vs. Planmine SMEST
Match: SMESG000074052.1 (SMESG000074052.1)

HSP 1 Score: 147.132 bits (370), Expect = 4.916e-36
Identity = 114/445 (25.62%), Postives = 212/445 (47.64%), Query Frame = 1 
Query:  340 CMKHALISRATDVATPKIDSCRLISDPSGYEVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKESA------NVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDATILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFY-----------NHTIEYENGQIP-------YSIEEKWGVGEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKHMLDCNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEISFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIID-SPNAKVEPFAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADRKRIIDSYIKVKNESNNIKNESRNDSQTFVENDSMT-LSSITESDSKQ 1596
             +KH  + + +D   P IDSC  I  P G ++++ F+SP   +E+ +  ++ +  Y     N    + +    ND +  S+      ++++SG T G  LK      F   DI +    +   +  +   + DD +++ + EHA  +  +  C   C Y       T  ++I +Y           N  +      +P       Y++  +W   EMP+WL  LL+D++++G + +G+FR++       ++   + + +D  I   I NKA I K F+  IEGSL+T  T++    L  +   I  E+F      Q+K +KL Q +P  +  LFQ+ILV+L+ II  S   ++    I+   + ++F  +       I+ L E  ++LT K+V P     I+ SY++ K+   +++N S+++    ++ +S +  +S T SDS +
Sbjct:  233 ALKHTYLDQCSDTQIPNIDSCLRIGGPFGNDMIIRFQSPLTRNEWFNYVNDEINSYQETIKNSTTTITI---FNDTVPSSSAPLVSRSITISGNTSGSSLKKMAMNTFEIKDIDVKDLSIVFLLKNKENVLHDDVSLMVLQEHAN-NVQENSCVKSCIYCD---KGTAAVKIIYYLRCNRPLNESMNKGLAQTKKPVPVKPKQNKYNMNVRWIDSEMPQWLMNLLADLFNEGVECEGIFRMSGSVFQSDEYVYQISETDDLKIDTTIHNKANILKKFISIIEGSLLTSITKEVFYNLSNEINSI--EDFLSQEDLQTKLLKLIQKIPANNLNLFQHILVILDTIIQKSQINQMNIQNISTCFSQNLFKHLSDDAMK-INKLFECILHLTSKLVNPVTYSNIVKSYVQRKSSVGSVENHSKSNGVGCIDQESNSPTTSRTVSDSNE 667
The following BLAST results are available for this feature:
BLAST of Rho GTPase-activating protein 20 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rga-24.500e-730.57Rho GTPase Activating protein [Source:UniProtKB/T... [more]
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
trhd2.910e-1028.21tumorhead [Source:Xenbase;Acc:XB-GENE-1214876][more]
gapvd11.622e-630.07GTPase activating protein and VPS9 domains 1 [Sour... [more]
gapvd12.639e-630.07GTPase activating protein and VPS9 domains 1 [Sour... [more]
gapvd12.901e-630.07GTPase activating protein and VPS9 domains 1 [Sour... [more]
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H0ZLY75.240e-1033.33Rho-GAP domain-containing protein OS=Taeniopygia g... [more]
U3K2921.040e-928.35Rho GTPase activating protein 20 OS=Ficedula albic... [more]
A0A218UTQ43.401e-933.33Rho GTPase-activating protein 20 OS=Lonchura stria... [more]
A0A093J0F87.553e-932.03Rho GTPase-activating protein 20 (Fragment) OS=Eur... [more]
A0A2P4T5M91.084e-830.48Uncharacterized protein (Fragment) OS=Bambusicola ... [more]
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000053633.11.813e-629.14rho GTPase-activating protein 20-like [Source:NCBI... [more]
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho GTPase-activating protein 20 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000074055.10.000e+099.20SMESG000074055.1[more]
SMESG000074055.10.000e+099.20SMESG000074055.1[more]
SMESG000074055.10.000e+099.18SMESG000074055.1[more]
SMESG000040021.12.643e-4428.39SMESG000040021.1[more]
SMESG000074052.14.916e-3625.62SMESG000074052.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031095 ID=SMED30031095|Name=Rho GTPase-activating protein 20|organism=Schmidtea mediterranea sexual|type=transcript|length=2407bp
TTTTTTTTATTCAAGTTTTAAGTAATATTTTGAATTATATTCATTTAAAT
TTATATCTAATTTGAGTAACATATTTTAATATTTAATTATTGTAATCAAA
TAAATATAATTGTTGTGCACAAGATTTTGATGCGAATGAATCAGAGTAAA
CTCAAAATTAACCCTTTCAATAAAATGGATAGAGACTTCAAATATGATGC
TCGTCAATTAATAAAAGTTTTTGACAAAGAATCAAAGTCTTATATTTTTA
ACGTCTCAGATATTAAAATCAAATTATCTAAAGATCGAGTTAAATTGTAC
TTAATTAAGAAGATTGAAGAAAAATTAATTCTTGATCTTTGCATGAAACA
TGCGTTGATTAGTCGAGCAACTGATGTTGCTACTCCGAAAATAGATTCTT
GTCGATTGATTAGTGATCCATCAGGCTATGAAGTTTTACTAAATTTTAAA
AGTCCATCAATTGCCAGTGAATATGAATCAAAATTCTCAGAAAGTATGAG
AATGTATAATACTAAATTTGCTAATATCTGTTTTGATGTAAATGTTACCA
ATTTGGTAAACGATAAATTGAAAGAATCAGCAAATGTTTCCCTATCTGGT
TTGACATTAGGTAAAGATCTTAAATCGTGGATTGAGCAAGAGTTTATATT
TCCTGATATCCACATGCCATGTTATATATTACAATTACACGTTGGAGAAG
AATTATTAACAATCCGAGATGACGCGACAATACTTCAAGTTCATGAGCAT
GCTGCTTATAGCCATGCCAAAGGTCACTGCTTCATAGGTTGTTTTTATGA
AAGCGTAAAACCAAACTCGACACTTTTGATAGAAATTAATTTCTATAATC
ACACAATTGAATATGAAAATGGCCAAATTCCGTATTCGATTGAAGAGAAA
TGGGGGGTTGGTGAAATGCCGAAATGGTTAACGAGATTACTGTCCGATAT
ATACTCCGATGGACCTGATTCTAAGGGGGTTTTCCGGTTGAATGCCAAGG
ATAGCTCGTTTTCAAAATGGTACAAACACATGCTTGATTGCAACGATTTT
ATAATTTCGGAAGATATCTTTAACAAAGCTGCTATTTTTAAATTTTTTTT
GAGAAATATTGAAGGAAGTTTAATGACAAAAAATACGAGAAAACAATTAA
ACGGTCTTTGTAAGAAATTCATGGAAATAAGTTTTGAGGAATTCTTTAAA
AATTCTAAAAAGCAATCAAAATTTATCAAACTATTCCAATCTCTCCCAAA
AACACATTATTTTTTATTTCAAAACATTCTGGTTGTCCTAAATGCGATAA
TAGATAGTCCAAATGCTAAAGTTGAGCCCTTTGCTATTGCCCGGGCAATT
GCGCCAAGTATTTTCCCTCTGGTCAGGTTAAGTTACTGGGGAAACATATC
GAACTTGTTGGAAGCGGCCATTTATCTGACAGATAAAATAGTCAAGCCCG
CAGATAGGAAGCGAATTATTGATTCTTATATTAAAGTGAAAAATGAGTCC
AATAACATAAAAAATGAAAGCCGAAATGATAGCCAAACGTTTGTTGAAAA
TGATTCCATGACTTTATCATCAATCACAGAATCGGATAGTAAACAGCAAA
ATTTGATCTCATGTGACAAACTATCGGATTGTGAATTGGAGAAAGAATCA
GATATTGAAAATTTTAGCCAAGATTCTATTCTGTCTGACAACGTCTCGTC
GTCGGACATTGAGAGCGACTCGTCATACAAATCCTGTGATTATCTGAAAA
AAGAAAACATTCAAAATCTTCCCCAAACCAGCTCTGAAAAACAATCAGAA
ACTGTCGATAGAAATATCTGTGAAATCGAATCAAGCGAAATGATTTCAAA
ATTACCAAATACCAAAAACATTTGTATGGGAGTAAAAATGAAACAAATCT
ATAGAAGTTGCAGCAAATTTACTGAATCAGAATTCCAAATTGAAAGTGAT
CACGTCAAAGAAAAATTATCACCTGTCTTGAGAAATGAGAAAAAAACTGT
TGGCCAAAGACATAAATCATTAATCCAATCTGATTCGTTAGGGAAAGCTC
TCGATCGAAACAATTTTGAATTCAACCAATCAGATTTCAATATGGAAAAA
GATAATATTAAAGAAAACTTTTCCCCAGTCTTAAGAAAAGAAACCAAAAA
TATTGGACAGAGACACAGATCTCTTATCCAACAGGATTCATCGATGAAAC
CGCTAGATGTCATGTCACCCATATCCAGTAGTTCGATGCCAGTAGAATTC
CCAGTAAAAAGAAAACACAGTCGAAAAGCTTTGTCAGCTGTTTCTGAACT
CAATAACAATAACAATCACCGTAGCCGTTCTCCATATGTTAAAAAACTGA
TTAAATTATACGAAAATAAAAAACAATAAAGTATTCCAGATGAATCTTAG
GAGGAGC
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protein sequence of SMED30031095-orf-1

>SMED30031095-orf-1 ID=SMED30031095-orf-1|Name=SMED30031095-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=750bp
MRMNQSKLKINPFNKMDRDFKYDARQLIKVFDKESKSYIFNVSDIKIKLS
KDRVKLYLIKKIEEKLILDLCMKHALISRATDVATPKIDSCRLISDPSGY
EVLLNFKSPSIASEYESKFSESMRMYNTKFANICFDVNVTNLVNDKLKES
ANVSLSGLTLGKDLKSWIEQEFIFPDIHMPCYILQLHVGEELLTIRDDAT
ILQVHEHAAYSHAKGHCFIGCFYESVKPNSTLLIEINFYNHTIEYENGQI
PYSIEEKWGVGEMPKWLTRLLSDIYSDGPDSKGVFRLNAKDSSFSKWYKH
MLDCNDFIISEDIFNKAAIFKFFLRNIEGSLMTKNTRKQLNGLCKKFMEI
SFEEFFKNSKKQSKFIKLFQSLPKTHYFLFQNILVVLNAIIDSPNAKVEP
FAIARAIAPSIFPLVRLSYWGNISNLLEAAIYLTDKIVKPADRKRIIDSY
IKVKNESNNIKNESRNDSQTFVENDSMTLSSITESDSKQQNLISCDKLSD
CELEKESDIENFSQDSILSDNVSSSDIESDSSYKSCDYLKKENIQNLPQT
SSEKQSETVDRNICEIESSEMISKLPNTKNICMGVKMKQIYRSCSKFTES
EFQIESDHVKEKLSPVLRNEKKTVGQRHKSLIQSDSLGKALDRNNFEFNQ
SDFNMEKDNIKENFSPVLRKETKNIGQRHRSLIQQDSSMKPLDVMSPISS
SSMPVEFPVKRKHSRKALSAVSELNNNNNHRSRSPYVKKLIKLYENKKQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR000198RhoGAP_dom
IPR008936Rho_GTPase_activation_prot
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 741..749
IPR000198Rho GTPase-activating protein domainSMARTSM00324RhoGAP_3coord: 261..442
e-value: 7.8E-4
score: 1.0
IPR000198Rho GTPase-activating protein domainPFAMPF00620RhoGAPcoord: 264..413
e-value: 4.1E-10
score: 39.7
IPR000198Rho GTPase-activating protein domainPROSITEPS50238RHOGAPcoord: 252..438
score: 14.863
IPR008936Rho GTPase activation proteinGENE3DG3DSA:1.10.555.10coord: 249..431
e-value: 1.6E-12
score: 49.1
IPR008936Rho GTPase activation proteinSUPERFAMILYSSF48350GTPase activation domain, GAPcoord: 262..414