Nucleobindin-2

Overview


Name	Nucleobindin-2
Smed ID	SMED30031061
Length (bp)	1226

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Nucleobindin-2 (SMED30031061) t-SNE clustered cells

Violin plots show distribution of expression levels for Nucleobindin-2 (SMED30031061) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nucleobindin-2 (SMED30031061) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nucleobindin-2 (SMED30031061) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30031061

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X2 cell	SMED30031061	h1SMcG0021063	SmedASXL_019494	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
Smed sexual biotype	SMED30031061	h1SMcG0021063	Contig49434	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30031061	h1SMcG0021063	Contig49434	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
epidermis	SMED30031061	h1SMcG0021063	dd_Smed_v4_6010_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30031061	h1SMcG0021063	dd_Smed_v4_6010_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30031061	h1SMcG0021063	dd_Smed_v4_6010_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30031061	h1SMcG0021063	SMED30031061	smed_20140614	PMID:26457503 Tu et al., 2015
parenchyma	SMED30031061	h1SMcG0021063	SMED30031061	smed_20140614	PMID:26457503 Tu et al., 2015
Category 2 cell	SMED30031061	h1SMcG0021063	dd_Smed_v6_6010_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
Category 3 cell	SMED30031061	h1SMcG0021063	dd_Smed_v6_6010_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Nucleobindin-2 vs. Ensembl Human
Match: NUCB1 (nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043])

HSP 1 Score: 132.109 bits (331), Expect = 1.193e-33
Identity = 100/283 (35.34%), Postives = 154/283 (54.42%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL +  L+ K   +++ N+ + +   L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  E+      ++LEE ++     R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   51 YHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRE-----LDFVSHHVRTKLDELKRQEVSRLRML-LKAKMDAEQDPNV-QVDHLNLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRH---REHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 318

[Close]

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL +  L+ K   +++ N+ + +   L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  E+      ++LEE ++     R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   51 YHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRE-----LDFVSHHVRTKLDELKRQEVSRLRML-LKAKMDAEQDPNV-QVDHLNLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRH---REHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 318

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Human
Match: NUCB1 (nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043])

HSP 1 Score: 120.939 bits (302), Expect = 9.194e-31
Identity = 95/283 (33.57%), Postives = 138/283 (48.76%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E E                                     H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  E+      ++LEE ++     R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   51 YHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRE-----LDFVSHHVRTKLDELKRQEFE-------------------------------------HLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRH---REHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 288

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Human
Match: NUCB2 (nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044])

HSP 1 Score: 120.939 bits (302), Expect = 2.560e-30
Identity = 94/284 (33.10%), Postives = 156/284 (54.93%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNL---AKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y EY++ V+  L TD  F+ K+        K G  S E     L+  S   R KLDE+KR+E+ RL +   AKL+       +L     +   L++       +H++  + +KF   D + LI     DL   D+ R +E++++E  K+ E ++ L+   + + + +   + ++ +K   NH  +N PGS++QLKE+W   D LD + F+ KTFF LHD+N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M ++DT+KD +VT EE+L
Sbjct:   54 YDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKE-----LDLVSHHVRTKLDELKRQEVGRLRMLIKAKLD-------SLQDIGMDHQALLKQF-----DHLNHLNPDKFESTDLDMLIKAATSDLEHYDKTRHEEFKKYEMMKEHERREYLKT-LNEEKRKEEESKFEEMKKKHENHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVTLEEFL 319

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Human
Match: NUCB2 (nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044])

HSP 1 Score: 121.324 bits (303), Expect = 6.330e-30
Identity = 94/284 (33.10%), Postives = 156/284 (54.93%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNL---AKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y EY++ V+  L TD  F+ K+        K G  S E     L+  S   R KLDE+KR+E+ RL +   AKL+       +L     +   L++       +H++  + +KF   D + LI     DL   D+ R +E++++E  K+ E ++ L+   + + + +   + ++ +K   NH  +N PGS++QLKE+W   D LD + F+ KTFF LHD+N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M ++DT+KD +VT EE+L
Sbjct:   54 YDEYLKQVIDVLETDKHFREKLQKADIEEIKSGRLSKE-----LDLVSHHVRTKLDELKRQEVGRLRMLIKAKLD-------SLQDIGMDHQALLKQF-----DHLNHLNPDKFESTDLDMLIKAATSDLEHYDKTRHEEFKKYEMMKEHERREYLKT-LNEEKRKEEESKFEEMKKKHENHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNSDGFLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVTLEEFL 319

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Celegans
Match: nucb-1 (NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc:Q8MQ51])

HSP 1 Score: 116.316 bits (290), Expect = 1.945e-28
Identity = 81/275 (29.45%), Postives = 159/275 (57.82%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y +Y++ V+  L +D +F  ++ + K     E+ + A N A     + +D++ +   ++L+ AK +    L++ +   + + D    ++ + D  H+   + EKF KED  KLI +   D+ ++D+ R+ +++++E KK  E   KL  +  P+ + K   E +++ K   +H+ L  PGS++QL+E+W   D L++  ++ KTFF LHD+N DG W+  E+ES+   E+ K+Y   N   D +E+  E+ +MR+H M+++D ++D +++ +E+L
Sbjct:   71 YTKYLEEVVKILESDPKFNERLKNMK-----EEDIKAGNIA-----DHIDDLPQEVFDKLHKAKFDEIERLRKQIE-EQIKADGGAHNIKMPD--HLDVQELEKFHKEDLRKLIQKTVADMNVMDEQRKDDFKQYEMKKQAEEDHKLA-QMTPEEREKAKSEHEESLKRHNDHEKLKHPGSKDQLQEVWEESDHLEKDQYDPKTFFALHDLNGDGFWNDFELESLFQLELEKMYNETNPDDDMKERAEEMYRMREHVMKQIDVNQDRMISMQEFL 331

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Celegans
Match: nucb-1 (NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc:Q8MQ51])

HSP 1 Score: 116.316 bits (290), Expect = 2.542e-28
Identity = 81/275 (29.45%), Postives = 159/275 (57.82%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y +Y++ V+  L +D +F  ++ + K     E+ + A N A     + +D++ +   ++L+ AK +    L++ +   + + D    ++ + D  H+   + EKF KED  KLI +   D+ ++D+ R+ +++++E KK  E   KL  +  P+ + K   E +++ K   +H+ L  PGS++QL+E+W   D L++  ++ KTFF LHD+N DG W+  E+ES+   E+ K+Y   N   D +E+  E+ +MR+H M+++D ++D +++ +E+L
Sbjct:   71 YTKYLEEVVKILESDPKFNERLKNMK-----EEDIKAGNIA-----DHIDDLPQEVFDKLHKAKFDEIERLRKQIE-EQIKADGGAHNIKMPD--HLDVQELEKFHKEDLRKLIQKTVADMNVMDEQRKDDFKQYEMKKQAEEDHKLA-QMTPEEREKAKSEHEESLKRHNDHEKLKHPGSKDQLQEVWEESDHLEKDQYDPKTFFALHDLNGDGFWNDFELESLFQLELEKMYNETNPDDDMKERAEEMYRMREHVMKQIDVNQDRMISMQEFL 331

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Celegans
Match: nucb-1 (NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc:Q8MQ51])

HSP 1 Score: 116.316 bits (290), Expect = 2.542e-28
Identity = 81/275 (29.45%), Postives = 159/275 (57.82%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y +Y++ V+  L +D +F  ++ + K     E+ + A N A     + +D++ +   ++L+ AK +    L++ +   + + D    ++ + D  H+   + EKF KED  KLI +   D+ ++D+ R+ +++++E KK  E   KL  +  P+ + K   E +++ K   +H+ L  PGS++QL+E+W   D L++  ++ KTFF LHD+N DG W+  E+ES+   E+ K+Y   N   D +E+  E+ +MR+H M+++D ++D +++ +E+L
Sbjct:   71 YTKYLEEVVKILESDPKFNERLKNMK-----EEDIKAGNIA-----DHIDDLPQEVFDKLHKAKFDEIERLRKQIE-EQIKADGGAHNIKMPD--HLDVQELEKFHKEDLRKLIQKTVADMNVMDEQRKDDFKQYEMKKQAEEDHKLA-QMTPEEREKAKSEHEESLKRHNDHEKLKHPGSKDQLQEVWEESDHLEKDQYDPKTFFALHDLNGDGFWNDFELESLFQLELEKMYNETNPDDDMKERAEEMYRMREHVMKQIDVNQDRMISMQEFL 331

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Fly
Match: NUCB1 (gene:FBgn0052190 transcript:FBtr0075171)

HSP 1 Score: 115.931 bits (289), Expect = 7.535e-28
Identity = 86/279 (30.82%), Postives = 155/279 (55.56%), Query Frame = 2

Query:  203 DYAEYMQSVMTELGTDIEFQNKVLDKKGS---RSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            +Y  Y++ V+  L  D EF+ K LDK      RS +     L++ +   R KLDEIKRRE+ERL            R L    YE    I    +  + H+   +   F  ED  KLI +   DL   D+ R  E++ +E +K+ E ++  + E D + + K   ELK+  +  ++H+ L+ PG++ QL+++W   D +D++ F+ KTFF++HD++ +G+WD  EV+++ + E+ K+Y++     D  E+  E+ +MR+H+ ++ D + D +++ +E++
Sbjct:   50 EYERYLREVVEALEADPEFRKK-LDKAPEADIRSGK-IAQELDYVNHHVRTKLDEIKRREVERL------------RELANQAYELSNDIDRKHLKVSQHLDHDNEHTFEIEDLRKLIQKTSDDLAEADRKRRGEFKEYEMQKEFE-REAQKKEMDEESRKKFETELKEKEEKHKDHEKLHHPGNKAQLEDVWEKQDHMDKNDFDPKTFFSIHDVDSNGYWDEAEVKALFVKELDKVYQSDLPEDDMRERAEEMERMREHYFQETDMNHDGLISIDEFM 313

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Zebrafish
Match: nucb1 (nucleobindin 1 [Source:ZFIN;Acc:ZDB-GENE-060825-222])

HSP 1 Score: 126.331 bits (316), Expect = 9.920e-32
Identity = 94/284 (33.10%), Postives = 149/284 (52.46%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIK---KKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+      R +LDE+KR+E+ RL +        LK  L+    +  ++  +  +    H+   +   F  +D E LI+    DL   D  R +E++R+E  K+ E +   K L+ EK  + + KR++ELK+     R H  +N PGS +QL+E+W   D LD   FN KTFF LHD N DG  D++E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +V+ EE+L
Sbjct:   44 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKE-----LDLVGHHVRTRLDELKRQEVSRLRM-------LLKAKLDSTNAQSVQMDHASLLKQFEHLDPHNQNTFEAKDLELLIATATKDLENYDAERHEEFKRYEMLKEHERREYLKSLDQEKR-EKEEKRMDELKEKH---RQHPKVNSPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNSDGVLDVQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMQNVDVNHDRLVSLEEFL 311

[Close]

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIK---KKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+      R +LDE+KR+E+ RL +        LK  L+    +  ++  +  +    H+   +   F  +D E LI+    DL   D  R +E++R+E  K+ E +   K L+ EK  + + KR++ELK+     R H  +N PGS +QL+E+W   D LD   FN KTFF LHD N DG  D++E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +V+ EE+L
Sbjct:   44 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKE-----LDLVGHHVRTRLDELKRQEVSRLRM-------LLKAKLDSTNAQSVQMDHASLLKQFEHLDPHNQNTFEAKDLELLIATATKDLENYDAERHEEFKRYEMLKEHERREYLKSLDQEKR-EKEEKRMDELKEKH---RQHPKVNSPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNSDGVLDVQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMQNVDVNHDRLVSLEEFL 311

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Zebrafish
Match: nucb2b (nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4775])

HSP 1 Score: 124.79 bits (312), Expect = 2.341e-31
Identity = 99/290 (34.14%), Postives = 161/290 (55.52%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTL---KRNLNINKYERDKLIRSLTVDDNN------HIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L  D  F+ K+ +      K+G  + E     L+F S + R KLDE+KR+E+ RL        RTL   K++LN  K         +TVD         H++  +   F  ED ++LI    +DL   D+ R +E++R+E  K+ E ++ L+   + + + K  E  ++ +K   +H  LN PGS++QLKE+W   D LD + F+ KTFFNLHD N DG++D +E+E++   E+ KIY+   +  D  E   E ++MR+H M ++DT+KD +V+ EE++
Sbjct:   48 YDRYLREVIDFLEKDPHFREKLHNTDMEDIKQGKLAKE-----LDFVSHNVRTKLDELKRQEVNRL--------RTLIKAKQDLNGEK--------GMTVDHQALLKQFEHLNHMNPHTFEVEDLDRLIKSATNDLENFDKERHEEFKRYEMMKEHERREHLKT-MNEEERKKEEEHYEEMKKKHADHPKLNHPGSQDQLKEVWEEADGLDPNDFDPKTFFNLHDTNGDGYFDEQELEALFTKELEKIYDPAQEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSLEEFI 315

[Close]

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTL---KRNLNINKYERDKLIRSLTVDDNN------HIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L  D  F+ K+ +      K+G  + E     L+F S + R KLDE+KR+E+ RL        RTL   K++LN  K         +TVD         H++  +   F  ED ++LI    +DL   D+ R +E++R+E  K+ E ++ L+   + + + K  E  ++ +K   +H  LN PGS++QLKE+W   D LD + F+ KTFFNLHD N DG++D +E+E++   E+ KIY+   +  D  E   E ++MR+H M ++DT+KD +V+ EE++
Sbjct:   48 YDRYLREVIDFLEKDPHFREKLHNTDMEDIKQGKLAKE-----LDFVSHNVRTKLDELKRQEVNRL--------RTLIKAKQDLNGEK--------GMTVDHQALLKQFEHLNHMNPHTFEVEDLDRLIKSATNDLENFDKERHEEFKRYEMMKEHERREHLKT-MNEEERKKEEEHYEEMKKKHADHPKLNHPGSQDQLKEVWEEADGLDPNDFDPKTFFNLHDTNGDGYFDEQELEALFTKELEKIYDPAQEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSLEEFI 315

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BLAST of Nucleobindin-2 vs. Ensembl Zebrafish
Match: nucb2a (nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-14])

HSP 1 Score: 122.479 bits (306), Expect = 3.832e-30
Identity = 93/281 (33.10%), Postives = 159/281 (56.58%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L  D  F+ K+ +      K+G  + E     L+F S   R+KLDE+KR+E+ RL    ++ K+ ++   +I   +   L++     ++ + H+     F  ED ++LI     DL   D+ R +E++++E  K+ E ++ L+   D D + K  E  ++ +K   +H  +N PGS++QLKE+W   D LD   F+ KTFFNLHD N DG +D +E+ES+   E+ KIY+  N+  D  E   E ++MR+H M ++D++KD +V+ +E+L
Sbjct:   53 YDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKE-----LDFVSHHVRSKLDELKRQEVSRLRTL-IKAKQDIEGGNDI-AVDHQALLKQFEYLNHMNPHT-----FEVEDLDRLIKSATKDLENYDKERHEEFKKYEMMKEHERREHLKT-LDEDGRKKEEEHYEEMKKKHADHPKVNHPGSKDQLKEVWEEADGLDPEDFDPKTFFNLHDTNGDGFFDEQELESLFTKELEKIYDPTNEEDDMVEMEEERLRMREHVMNEVDSNKDRLVSLDEFL 320

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BLAST of Nucleobindin-2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 129.798 bits (325), Expect = 3.305e-33
Identity = 98/284 (34.51%), Postives = 153/284 (53.87%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN-LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEE--LKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL  L K ++  T++ N+ I+      L++        H+   +   F   D E LI     DL   D AR +E++R+E  K+ E ++ L   K  D + +++EE   ++ +K  + H  +N PGS +QLKE+W   D LD + FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +VT +E+L
Sbjct:   44 YDRYLREVIDVLETDGHFREKLQAANADDIKSGKLSKE-----LDFVSHHVRTKLDELKRQEVSRLRMLIKAKMDATMEENVQIDHM---SLLKQF-----EHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYL---KSLDEEKRKMEEAHFEEMKKKHKEHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFL 311

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 130.183 bits (326), Expect = 4.059e-33
Identity = 99/284 (34.86%), Postives = 152/284 (53.52%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN-LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL  L K ++  T++ N+ I+      L++        H+   +   F   D E LI     DL   D AR +E++R+E  K+ E ++ L++  E+   ++    EE+KK  K    H  +N PGS +QLKE+W   D LD + FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +VT +E+L
Sbjct:   26 YDRYLREVIDVLETDGHFREKLQAANADDIKSGKLSKE-----LDFVSHHVRTKLDELKRQEVSRLRMLIKAKMDATMEENVQIDHM---SLLKQF-----EHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDEEKRKMEEAHFEEMKKKHK---EHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFL 293

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 130.183 bits (326), Expect = 5.174e-33
Identity = 99/284 (34.86%), Postives = 152/284 (53.52%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN-LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL  L K ++  T++ N+ I+      L++        H+   +   F   D E LI     DL   D AR +E++R+E  K+ E ++ L++  E+   ++    EE+KK  K    H  +N PGS +QLKE+W   D LD + FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +VT +E+L
Sbjct:   44 YDRYLREVIDVLETDGHFREKLQAANADDIKSGKLSKE-----LDFVSHHVRTKLDELKRQEVSRLRMLIKAKMDATMEENVQIDHM---SLLKQF-----EHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDEEKRKMEEAHFEEMKKKHK---EHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFL 311

[Close]

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN-LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL  L K ++  T++ N+ I+      L++        H+   +   F   D E LI     DL   D AR +E++R+E  K+ E ++ L++  E+   ++    EE+KK  K    H  +N PGS +QLKE+W   D LD + FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +VT +E+L
Sbjct:   44 YDRYLREVIDVLETDGHFREKLQAANADDIKSGKLSKE-----LDFVSHHVRTKLDELKRQEVSRLRMLIKAKMDATMEENVQIDHM---SLLKQF-----EHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDEEKRKMEEAHFEEMKKKHK---EHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFL 311

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Xenopus
Match: nucb1 (nucleobindin 1 [Source:NCBI gene;Acc:407884])

HSP 1 Score: 129.798 bits (325), Expect = 6.390e-33
Identity = 99/284 (34.86%), Postives = 152/284 (53.52%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN-LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL  L K ++  T++ N+ I+      L++        H+   +   F   D E LI     DL   D AR +E++R+E  K+ E ++ L++  E+   ++    EE+KK  K    H  +N PGS +QLKE+W   D LD + FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D + D +VT +E+L
Sbjct:   14 YDRYLREVIDVLETDGHFREKLQAANADDIKSGKLSKE-----LDFVSHHVRTKLDELKRQEVSRLRMLIKAKMDATMEENVQIDHM---SLLKQF-----EHLDPQNQHTFEARDLELLIQAATKDLENYDAARHEEFKRYEMMKEHERREYLKSLDEEKRKMEEAHFEEMKKKHK---EHPKVNVPGSIDQLKEVWEETDGLDPNEFNPKTFFKLHDTNGDGVLDEQELEALFTKELEKVYDPKNEEDDMVEMEEERLRMREHVMKNVDANHDRLVTLDEFL 281

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Mouse
Match: Nucb1 (nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388])

HSP 1 Score: 135.191 bits (339), Expect = 7.037e-35
Identity = 101/287 (35.19%), Postives = 158/287 (55.05%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNL---AKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN---EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S + R KLDE+KR+E+ RL +   AK++ K+  + NL ++      L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+   E+  + + K  E+ ++ R+ P+    +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   50 YHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQE-----LDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQ--EPNLQVDHM---NLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPK----VNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 317

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Mouse
Match: Nucb1 (nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388])

HSP 1 Score: 135.191 bits (339), Expect = 7.369e-35
Identity = 101/287 (35.19%), Postives = 158/287 (55.05%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNL---AKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN---EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S + R KLDE+KR+E+ RL +   AK++ K+  + NL ++      L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+   E+  + + K  E+ ++ R+ P+    +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   50 YHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQE-----LDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQ--EPNLQVDHM---NLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPK----VNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 317

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Mouse
Match: Nucb2 (nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179])

HSP 1 Score: 117.472 bits (293), Expect = 9.322e-29
Identity = 89/281 (31.67%), Postives = 148/281 (52.67%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y EY++ V+  L TD  F+ K+        + G  S E     L+  S   R +LDE+KR+E+ RL +  ++ K    ++  +N +        L +    H++  +   F   D + LI     DL   D+ R +E++++E  K+ E ++ L+   +   K +  +  +  RK   +H  +N PGS++QLKE+W   D LD + F+ KTFF LHD+N DG  D +E+E++   E+ K+Y   N   D  E   E ++MR+H M ++D +KD +VT EE+L
Sbjct:   54 YDEYLKQVIEVLETDPHFREKLQKADIEEIRSGRLSQE-----LDLVSHKVRTRLDELKRQEVGRLRML-IKAKLDALQDTGMNHH--------LLLKQFEHLNHQNPNTFESRDLDMLIKAATADLEQYDRTRHEEFKKYEMMKEHERREYLKTLSEEKRKEEESKFEEMKRKH-EDHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFL 319

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Mouse
Match: Nucb2 (nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179])

HSP 1 Score: 117.472 bits (293), Expect = 9.697e-29
Identity = 89/281 (31.67%), Postives = 148/281 (52.67%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y EY++ V+  L TD  F+ K+        + G  S E     L+  S   R +LDE+KR+E+ RL +  ++ K    ++  +N +        L +    H++  +   F   D + LI     DL   D+ R +E++++E  K+ E ++ L+   +   K +  +  +  RK   +H  +N PGS++QLKE+W   D LD + F+ KTFF LHD+N DG  D +E+E++   E+ K+Y   N   D  E   E ++MR+H M ++D +KD +VT EE+L
Sbjct:   54 YDEYLKQVIEVLETDPHFREKLQKADIEEIRSGRLSQE-----LDLVSHKVRTRLDELKRQEVGRLRML-IKAKLDALQDTGMNHH--------LLLKQFEHLNHQNPNTFESRDLDMLIKAATADLEQYDRTRHEEFKKYEMMKEHERREYLKTLSEEKRKEEESKFEEMKRKH-EDHPKVNHPGSKDQLKEVWEETDGLDPNDFDPKTFFKLHDVNNDGFLDEQELEALFTRELEKVYNPQNAEDDMIEMEEERLRMREHVMSEIDNNKDRLVTLEEFL 319

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Mouse
Match: Nucb1 (nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388])

HSP 1 Score: 101.679 bits (252), Expect = 4.516e-24
Identity = 62/156 (39.74%), Postives = 97/156 (62.18%), Query Frame = 2

Query:  563 DLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            DL   D A  +E++R+E  K+ E ++ LE+  +   K     +L++ ++  R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   30 DLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAE-RKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 184

[Close]

BLAST of Nucleobindin-2 vs. UniProt/SwissProt
Match: sp|Q0P569|NUCB1_BOVIN (Nucleobindin-1 OS=Bos taurus OX=9913 GN=NUCB1 PE=1 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.511e-34
Identity = 101/283 (35.69%), Postives = 153/283 (54.06%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL +  L+ K   ++  NI + +   L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  E+      ++LEE ++     R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   48 YHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSRE-----LDFVSHHVRTKLDELKRQEVSRLRML-LKAKMDAQQEPNI-QLDHLNLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRH---REHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEDDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 315

[Close]

BLAST of Nucleobindin-2 vs. UniProt/SwissProt
Match: sp|Q63083|NUCB1_RAT (Nucleobindin-1 OS=Rattus norvegicus OX=10116 GN=Nucb1 PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.952e-34
Identity = 100/284 (35.21%), Postives = 155/284 (54.58%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNL---AKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S + R KLDE+KR+E+ RL +   AK++ K+  + NL ++      L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  +   K     +L++ ++  R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   50 YHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQE-----LDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQ--EPNLQVDHM---NLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAE-RKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 317

[Close]

BLAST of Nucleobindin-2 vs. UniProt/SwissProt
Match: sp|Q02819|NUCB1_MOUSE (Nucleobindin-1 OS=Mus musculus OX=10090 GN=Nucb1 PE=1 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 5.155e-34
Identity = 101/287 (35.19%), Postives = 158/287 (55.05%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNL---AKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN---EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S + R KLDE+KR+E+ RL +   AK++ K+  + NL ++      L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+   E+  + + K  E+ ++ R+ P+    +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   50 YHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQE-----LDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQ--EPNLQVDHM---NLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPK----VNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 317

[Close]

BLAST of Nucleobindin-2 vs. UniProt/SwissProt
Match: sp|Q02818|NUCB1_HUMAN (Nucleobindin-1 OS=Homo sapiens OX=9606 GN=NUCB1 PE=1 SV=4)

HSP 1 Score: 132.109 bits (331), Expect = 5.727e-33
Identity = 100/283 (35.34%), Postives = 154/283 (54.42%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL +  L+ K   +++ N+ + +   L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  E+      ++LEE ++     R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   51 YHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRE-----LDFVSHHVRTKLDELKRQEVSRLRML-LKAKMDAEQDPNV-QVDHLNLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRH---REHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFL 318

[Close]

BLAST of Nucleobindin-2 vs. UniProt/SwissProt
Match: sp|Q5R4U1|NUCB1_PONAB (Nucleobindin-1 OS=Pongo abelii OX=9601 GN=NUCB1 PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 1.018e-32
Identity = 100/283 (35.34%), Postives = 153/283 (54.06%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y+Q V+  L TD  F+ K+        K G  S E     L+F S   R KLDE+KR+E+ RL +  L+ K   +++ N+ + +   L++        H+   +   F   D E LI     DL   D A  +E++R+E  K+ E ++ LE+  E+      +RLEE ++     R H  +N PGS+ QLKE+W   D LD + FN KTFF LHDIN DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +DT++D +VT  E+L
Sbjct:   51 YHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRE-----LDFVSHHVRTKLDELKRQEVSRLRML-LKAKMDAEQDPNV-QVDHLNLLKQF-----EHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERRLEEQQRRH---REHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLGEFL 318

[Close]

BLAST of Nucleobindin-2 vs. TrEMBL
Match: B7Q350 (Niemann-Pick type C1 domain-containing protein, putative OS=Ixodes scapularis OX=6945 GN=8035180 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.605e-45
Identity = 98/279 (35.13%), Postives = 165/279 (59.14%), Query Frame = 2

Query:  203 DYAEYMQSVMTELGTDIEFQNKVLDKK-GSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLEN--EKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            +Y  Y+Q V+  L  D EF  K+ +       + D    L F   S RN+LDE+KR E++RL   +L +K   ++   +   +RD++   L VD  N  HS     F  +D +KLI     DL  +D+ R++E++ +E +K+LE + KLEN  E++   + +  EELK+  K   +H  ++EPGS+ QL+E W   D +++  F+  TFF +HD+N DG  D +E+E++L PEV K+Y+  N+  DP E+  EI +MR+H + ++ ++KD ++++EE++
Sbjct: 1211 EYDRYLQEVVRALEDDAEFAAKLRNVSIDDIKSGDVAHQLEFVKHSVRNRLDELKRIEVDRLR--RLTVKAMERKEFGL---DRDQVKMPLHVDYQNP-HS-----FEIDDLKKLIVTATKDLEKLDEKRKEEFKEYEMQKELEYRHKLENMTEEEKKKEQQHHEELKRKHK---DHPAVHEPGSKPQLEETWEKQDHMNKEDFDPNTFFAMHDLNGDGQLDQDEIEAILTPEVKKVYDPNNEEDDPRERQEEIQRMREHVVHEVSSNKDGMISHEEFM 1475

[Close]

BLAST of Nucleobindin-2 vs. TrEMBL
Match: A0A1I8J719 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.010e-42
Identity = 101/280 (36.07%), Postives = 161/280 (57.50%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDD-----NNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTG-DPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEY 1027
            Y +Y+  V++ + +D EF N++   +    +++    L FAS S RN+LDEIKR+E+ERLN  +  L+  L    N  K  R K    L   D       H+  ++   F   D +KLI+Q+  D+  VDQARE EY+R+E  K+   + +L   KD   +    ++++  R+  R H+ +++PGS EQLKE W   D++D   F+ K FF LHDIN DGH D+ E+E++   E+ K+Y   N T  D  E+ HE+ +MR+  + ++D +KD +V++EE+
Sbjct:   46 YRQYLSEVISAMESDKEFANRL---RSMNDSDEMANQLEFASHSVRNRLDEIKRQEVERLNELEKALEDKLALKANPGKASRAKFRFGLQSLDEATIKRAHLDHSNPHTFEIADLQKLINQVTQDIDQVDQAREAEYKRYEMGKEHSRRARLRRIKDKKAREAAEQQMRAERERHRRHERVHQPGSREQLKEAWEKGDRMDRDSFDPKAFFMLHDINGDGHMDVNEIEALFWKELDKVYSKENDTTVDRVERQHEMERMRRAVLMRLDKNKDRMVSWEEF 322

[Close]

BLAST of Nucleobindin-2 vs. TrEMBL
Match: A0A267G7U2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034413g5 PE=4 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.102e-42
Identity = 101/280 (36.07%), Postives = 161/280 (57.50%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDD-----NNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTG-DPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEY 1027
            Y +Y+  V++ + +D EF N++   +    +++    L FAS S RN+LDEIKR+E+ERLN  +  L+  L    N  K  R K    L   D       H+  ++   F   D +KLI+Q+  D+  VDQARE EY+R+E  K+   + +L   KD   +    ++++  R+  R H+ +++PGS EQLKE W   D++D   F+ K FF LHDIN DGH D+ E+E++   E+ K+Y   N T  D  E+ HE+ +MR+  + ++D +KD +V++EE+
Sbjct:   48 YRQYLSEVISAMESDKEFANRL---RSMNDSDEMANQLEFASHSVRNRLDEIKRQEVERLNELEKALEDKLALKANPGKASRAKFRFGLQSLDEATIKRAHLDHSNPHTFEIADLQKLINQVTQDIDQVDQAREAEYKRYEMGKEHSRRARLRRIKDKKAREAAEQQMRAERERHRRHERVHQPGSREQLKEAWEKGDRMDRDSFDPKAFFMLHDINGDGHMDVNEIEALFWKELDKVYSKENDTTVDRVERQHEMERMRRAVLMRLDKNKDRMVSWEEF 324

[Close]

BLAST of Nucleobindin-2 vs. TrEMBL
Match: A0A267DJS5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034413g1 PE=4 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 2.408e-42
Identity = 101/280 (36.07%), Postives = 161/280 (57.50%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDD-----NNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTG-DPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEY 1027
            Y +Y+  V++ + +D EF N++   +    +++    L FAS S RN+LDEIKR+E+ERLN  +  L+  L    N  K  R K    L   D       H+  ++   F   D +KLI+Q+  D+  VDQARE EY+R+E  K+   + +L   KD   +    ++++  R+  R H+ +++PGS EQLKE W   D++D   F+ K FF LHDIN DGH D+ E+E++   E+ K+Y   N T  D  E+ HE+ +MR+  + ++D +KD +V++EE+
Sbjct:   48 YRQYLSEVISAMESDKEFANRL---RSMNDSDEMANQLEFASHSVRNRLDEIKRQEVERLNELEKALEDKLALKANPGKASRAKFRFGLQSLDEATIKRAHLDHSNPHTFEIADLQKLINQVTQDIDQVDQAREAEYKRYEMGKEHSRRARLRRIKDKKAREAAEQQMRAERERHRRHERVHQPGSREQLKEAWEKGDRMDRDSFDPKAFFMLHDINGDGHMDVNEIEALFWKELDKVYSKENDTTVDRVERQHEMERMRRAVLMRLDKNKDRMVSWEEF 324

[Close]

BLAST of Nucleobindin-2 vs. TrEMBL
Match: A0A267G656 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig034413g4 PE=4 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.972e-42
Identity = 101/280 (36.07%), Postives = 160/280 (57.14%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDD-----NNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTG-DPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEY 1027
            Y +Y+  V++ + +D EF N++   +    +++    L FAS S RN+LDEIKR+E+ERLN  +  L+  L    N  K  R K    L   D       H+  +    F   D +KLI+Q+  D+  VDQARE EY+R+E  K+   + +L   KD   +    ++++  R+  R H+ +++PGS EQLKE W   D++D   F+ K FF LHDIN DGH D+ E+E++   E+ K+Y   N T  D  E+ HE+ +MR+  + ++D +KD +V++EE+
Sbjct:   48 YRQYLSEVISAMESDKEFANRL---RSMNDSDEMANQLEFASHSVRNRLDEIKRQEVERLNELEKALEDKLALKANPGKASRAKFRFGLQSLDEATIKRAHLDHSKPHTFEIADLQKLINQVTQDIDQVDQAREAEYKRYEMGKEHSRRARLRRIKDKKAREAAEQQMRAERERHRRHERVHQPGSREQLKEAWEKGDRMDRDSFDPKAFFMLHDINGDGHMDVNEIEALFWKELDKVYSKENDTTVDRVERQHEMERMRRAVLMRLDKNKDRMVSWEEF 324

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Cavefish
Match: nucb1 (nucleobindin-2-like [Source:NCBI gene;Acc:103046537])

HSP 1 Score: 112.849 bits (281), Expect = 4.796e-27
Identity = 93/284 (32.75%), Postives = 148/284 (52.11%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIK---KKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+      R +LDE+KR+E+ RL +        LK  L+    +  ++     +    H+   +   F  +D E LI+    DL   D  R +E++R+E  K+ E +   K L+ EK  + + KR+E+L   R+  R H  +N PGS +QL+E+W   D LD   FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+ +D ++D +V+ EE++
Sbjct:   49 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKE-----LDLVGHHVRTRLDELKRQEVSRLRM-------LLKAKLDSTNTQSVQMDHVSLLKQFEHLDPHNQNTFEAKDLELLIAAATKDLENYDAERHEEFKRYEMLKEHERREYLKSLDQEKR-EREEKRIEDL---REKHRQHPKVNAPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNGDGLLDEQELEALFTKELEKVYDPKNEEDDMMEMEEERLRMREHVMKNVDLNRDRLVSLEEFI 316

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Cavefish
Match: nucb1 (nucleobindin-2-like [Source:NCBI gene;Acc:103046537])

HSP 1 Score: 103.605 bits (257), Expect = 7.050e-24
Identity = 92/282 (32.62%), Postives = 141/282 (50.00%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIK---KKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEE 1024
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+      R +LDE+KR+E+ RL +        LK  L+    +  ++     +    H+   +   F  +D E LI+    DL   D  R +E++R+E  K+ E +   K L+ EK  + + KR+E+L   R+  R H  +N PGS +QL+E+W   D LD   FN KTFF LHD N DG  D +E+E++   E+ K+Y+  N+  D  E   E ++MR+H M+    DK  + T  E
Sbjct:   49 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKE-----LDLVGHHVRTRLDELKRQEVSRLRM-------LLKAKLDSTNTQSVQMDHVSLLKQFEHLDPHNQNTFEAKDLELLIAAATKDLENYDAERHEEFKRYEMLKEHERREYLKSLDQEKR-EREEKRIEDL---REKHRQHPKVNAPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNGDGLLDEQELEALFTKELEKVYDPKNEEDDMMEMEEERLRMREHVMKNTLDDKQPVYTEAE 314

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Cavefish
Match: nucb2a (nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-14])

HSP 1 Score: 51.6026 bits (122), Expect = 7.967e-7
Identity = 70/281 (24.91%), Postives = 127/281 (45.20%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L  D  F+ K+ +      K+G  + E     L+F S   R KLDE+KR+E+ RL    ++ K+ ++   N    +   L++     ++ + H+     F  ED ++LI     DL   D+ R +E++++E  K+ E ++ L+   D + + K  E  ++ +K   +H  +N P                    F LK  + L                    E+ KIY+  N+  D  E   E ++MR+H M ++D +KD +V+ +E+L
Sbjct:   53 YDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKE-----LDFVSHHVRTKLDELKRQEVSRLRTL-IKAKQDVEEG-NGMAVDHQALLKQFEYLNHMNPHT-----FEVEDLDRLIKSATSDLENFDKERHEEFKKYEMMKEHERREHLKT-LDEEGRKKEEEHYEEMKKKHADHPKVNHP------------------VRFILKLIYTL-------------------TELEKIYDPTNEEDDMVEMEEERLRMREHVMNEVDANKDRLVSLDEFL 283

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Sea Lamprey
Match: nucb2b (nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4775])

HSP 1 Score: 83.9593 bits (206), Expect = 1.067e-18
Identity = 68/215 (31.63%), Postives = 113/215 (52.56%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNK-----VLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN-LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLH 832
            Y  Y++ V+  L  D  F+ K     + D K  R +++    L+F S   R+KLDE+KR+E+ RL  L K ++     ++L ++      L++     D+N+ HS     F  +D EKLI     DL   D AR +E++R+E  K+ E ++ L+   D + + +  +     ++  ++H  +N PGS++QL+E+W   D LD   F  KTFF LH
Sbjct:   60 YDRYLREVIDVLEKDTHFKQKLETADIEDIKNGRLSKE----LDFVSHHVRSKLDELKRQEVARLRMLIKAKMDAAQGKDLRMD---HQSLLKQFEHLDHNNPHS-----FEPQDLEKLIKTATKDLENYDAARHEEFKRYEMLKEHERREFLKT-LDEERRQEEEKRFLDMKQKHKDHPKVNHPGSKDQLQEVWEESDGLDPEQFEPKTFFKLH 261

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Nematostella
Match: EDO43098 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZI2])

HSP 1 Score: 125.946 bits (315), Expect = 1.623e-32
Identity = 93/285 (32.63%), Postives = 156/285 (54.74%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVL-----DKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLN--INKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNH---KPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            YA Y++ V+  L  D ++  K++     D +  R  ED    ++      R+KLDE+KR+E+ER        +R ++R +N  +N  +  +    L  D+N          F  +DF+KL+ +   ++   D+ R +E+++HE +K+ E K K+   KD D K ++  E        ++H   K L+ PGS+ QL+++W   D LD   F+ KTFF LHD+N DG  D  E+E++ + EV K+Y   ++  DP+E+  EI +MR+H M ++D DKD  V+ +E+L
Sbjct:   44 YARYLRQVIEILEKDEDYVRKLMNASDDDLRSGRIAED----IDLVKHDVRSKLDELKRQEVER--------QRMIRRQMNDHLNGIKEREYWNPLFDDENPDF-------FGADDFKKLLWKHHEEMDKQDRERREEFKKHEMQKEHERKIKM---KDMDEKHRKEAEEHFRELQEKHHNQSKQLHHPGSKAQLEQVWEESDGLDAKDFDPKTFFKLHDVNGDGFLDTGELEALFVKEVTKLYNPKDEDYDPKERDEEISRMREHVMNEIDKDKDGFVSQDEFL 306

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Medaka
Match: nucb2a (nucleobindin-2 [Source:NCBI gene;Acc:101163155])

HSP 1 Score: 124.79 bits (312), Expect = 4.230e-31
Identity = 95/281 (33.81%), Postives = 157/281 (55.87%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L  D  F+ K+ +      K+G  + E     L+F S   R KLDE+KR+E+ RL    ++ K+ ++   +I   +   L++     ++ + H+     F  ED ++LI     DL   D+ R +E++R+E  K+ + ++ L+   D + K K  E  ++ RK   +H  +N PGSE QLKE+W   D LD   F+ KTFFNLHD N DG +D +E+E++   E+ KIY+  N+  D  E   E ++MR+H M ++DT+KD +V+ +E+L
Sbjct:   63 YDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKE-----LDFVSHHVRTKLDELKRQEVNRLRTL-IKAKQDIEGGNDI-AVDHQALLKQFEYLNHMNPHT-----FEVEDLDRLIKSATKDLENYDKERHEEFKRYEMMKEHDRREHLKTLNDEERK-KEEEHYEEMRKKHADHPKVNHPGSENQLKEVWEEADGLDPEDFDPKTFFNLHDTNGDGFFDEQELEALFTKELEKIYDPTNEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSMDEFL 330

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Medaka
Match: NUCB2 (nucleobindin 2 [Source:NCBI gene;Acc:101162363])

HSP 1 Score: 123.25 bits (308), Expect = 4.266e-31
Identity = 92/283 (32.51%), Postives = 151/283 (53.36%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKS--KRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L  D  F+ K+ +      K+G  S E     LNF   + R+KLDE+KR EI RL +    L +        N  +   L++        H++  +   F  +D ++LI     DL   D+ R  E++++E  K+ E +++L+   + D K   +  EE+KK      NH  +N PGSE+QLKE+W+  D LD   F+ KTFF +HD N DG +D  E+E++   E+ K+Y   N+  D  E   E ++M++H M ++DT+KD +V++ E++
Sbjct:   52 YDRYLREVIEYLEKDPHFREKLKNADMDDIKQGKLSKE-----LNFVQHNFRSKLDELKREEINRLRM----LIKAKHDTEEHNAMDHQTLLKQF-----EHLNHMNPHTFEVDDLDRLIQSATKDLENFDKERHDEFKKYELLKEHERRERLKGMSEEDRKKAEQHYEEMKKKHA---NHPKVNHPGSEDQLKEVWQETDGLDPQDFDPKTFFKMHDSNGDGFFDESELEALFTKELEKVYNPDNEEDDMVEMEEERLRMKEHVMNEVDTNKDRLVSFSEFM 317

[Close]

BLAST of Nucleobindin-2 vs. Ensembl Medaka
Match: nucb1 (nucleobindin-2 [Source:NCBI gene;Acc:101169036])

HSP 1 Score: 120.553 bits (301), Expect = 8.302e-30
Identity = 97/284 (34.15%), Postives = 150/284 (52.82%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLD------KKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIK---KKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y  Y++ V+  L TD  F+ K+        K G  S E     L+      R +LDE+KR+E+ RL +        LK  L+    +  ++  +  +    H+   +   F  +D E LIS    DL   D  R +E++R+E  K+ E +   K L+ EK  + + KR++ELK+     R H  +N PGS +QL+E+W   D LD   FN KTFF LHD N+DG  D +E+E++   E+ K+Y   N+  D  E   E ++MR+H M+ +DT++D +VT EE+L
Sbjct:   47 YDRYLREVIEVLETDPHFREKLQTANTEDIKNGRLSKE-----LDLVGHHVRTRLDELKRQEVSRLRM-------LLKAKLDSTNTQSLQMDHASLLKQFEHLDPHNQNTFEAKDLELLISAATKDLENYDAERHEEFKRYEMLKEHERREYLKSLDQEKR-EKEEKRIQELKEKH---RQHPKVNAPGSVDQLREVWEETDGLDPQEFNPKTFFKLHDTNEDGVLDEQELEALFTKELEKVYNPKNEEDDMMEMEEERLRMREHVMKNVDTNRDRLVTLEEFL 314

[Close]

BLAST of Nucleobindin-2 vs. Planmine SMEST
Match: SMESG000017142.1 (SMESG000017142.1)

HSP 1 Score: 552.747 bits (1423), Expect = 0.000e+0
Identity = 312/314 (99.36%), Postives = 312/314 (99.36%), Query Frame = 2

Query:  218 MQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYLXXXXXXXXXXXXXXXVTEEQDNXXXXXXXXXXXXXXXXXVPKR 1159
            MQSVMTELGTDIEFQNKVLDKKGS SNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYLEFSKKKEFK NKEWKVTEEQDNEIYSNEDIEEFEEELKIVPKR
Sbjct:    1 MQSVMTELGTDIEFQNKVLDKKGSMSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYLEFSKKKEFKINKEWKVTEEQDNEIYSNEDIEEFEEELKIVPKR 314

[Close]

BLAST of Nucleobindin-2 vs. Planmine SMEST
Match: SMESG000016720.1 (SMESG000016720.1)

HSP 1 Score: 344.354 bits (882), Expect = 2.150e-116
Identity = 169/284 (59.51%), Postives = 225/284 (79.23%), Query Frame = 2

Query:  179 FFSELNQKDYAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            F  ELNQK+Y+EYM++VM EL  D   Q K+ ++  S SN+D++ AL+ ASSS+RNKLDEIK REI RLN  KL+LKR LK +  ++K ERD++  +LT     H+HST++ KFTK+D EKL+SQI+HDLRLVD  RE++YRR+EWKKD  ++K+LE  KDP  KSKRLEE+KK+RK+ R HK +NEPGSE+Q KE+W++ D LD SYFN+KTFFNLHD+NQDG+WDI E+E+++ P++ KIYE  N T DPEEK+HEIMKMR+H MEK+D DKD+++T +E +
Sbjct:   28 FIDELNQKEYSEYMRNVMQELLKDKNIQGKIFNQ-NSTSNKDFMDALDLASSSSRNKLDEIKSREINRLNSLKLQLKRMLKSSTKLSKNERDEMADNLTSYRRTHLHSTEDSKFTKDDMEKLMSQIRHDLRLVDTVREEQYRRYEWKKDWRLRKELEKFKDPVQKSKRLEEIKKSRKSYRKHKRINEPGSEDQFKEIWKSVDNLDISYFNIKTFFNLHDLNQDGNWDINEIEAVVQPDIEKIYEDANGTADPEEKFHEIMKMRQHLMEKIDNDKDDLITLQELI 310

[Close]

BLAST of Nucleobindin-2 vs. Planmine SMEST
Match: SMESG000000846.1 (SMESG000000846.1)

HSP 1 Score: 174.096 bits (440), Expect = 8.156e-50
Identity = 117/291 (40.21%), Postives = 179/291 (61.51%), Query Frame = 2

Query:  167 EKVTFFSELNQKDYAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYE---RDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            E+   F++L+   Y EY+  ++ EL +D  F  ++ D +     E    +L F     R++LDEIKRREIERLN+ + E++R   R L    Y+    D  ++S+   D  H+   +   F   D EKLI +++ DL  +D+ +EK+++ HE KK+   KKKLE  KDP+ + K  E++K++    +NH  ++ PGSEEQLKE+W  +D LD   FN K FF+LHD+N DGH DI E+ES++  E+ K+YEA N   D +EKYHE+M+MR   M+ +D +KD +V+ +E+L
Sbjct:   29 EESNLFNDLH---YKEYLDEIIKELESDKNFAERIKDAQAHNRTEHIAQSLQFVHHRIRSRLDEIKRREIERLNVLENEIER---RRLEAKGYKVPPPDPKLKSVISFDKEHLDHNNVHTFEMSDLEKLIKKVEDDLSQIDKEQEKQFQSHEMKKEHLKKKKLETIKDPNERKKVEEQMKESEIRHKNHSKVHRPGSEEQLKEVWDKNDHLDPKEFNPKAFFDLHDVNGDGHLDINEIESLMQNELSKVYEANNSDDDMQEKYHEMMRMRLEMMKVIDKNKDNLVSKDEFL 313

[Close]

BLAST of Nucleobindin-2 vs. Planmine SMEST
Match: SMESG000000846.1 (SMESG000000846.1)

HSP 1 Score: 173.326 bits (438), Expect = 2.318e-49
Identity = 114/278 (41.01%), Postives = 172/278 (61.87%), Query Frame = 2

Query:  206 YAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYE---RDKLIRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            Y EY+  ++ EL +D  F  ++ D +     E    +L F     R++LDEIKRREIERLN+ + E++R   R L    Y+    D  ++S+   D  H+   +   F   D EKLI +++ DL  +D+ +EK+++ HE KK+   KKKLE  KDP+ + K  E++K++    +NH  ++ PGSEEQLKE+W  +D LD   FN K FF+LHD+N DGH DI E+ES++  E+ K+YEA N   D +EKYHE+M+MR   M+ +D +KD +V+ +E+L
Sbjct:   56 YKEYLDEIIKELESDKNFAERIKDAQAHNRTEHIAQSLQFVHHRIRSRLDEIKRREIERLNVLENEIER---RRLEAKGYKVPPPDPKLKSVISFDKEHLDHNNVHTFEMSDLEKLIKKVEDDLSQIDKEQEKQFQSHEMKKEHLKKKKLETIKDPNERKKVEEQMKESEIRHKNHSKVHRPGSEEQLKEVWDKNDHLDPKEFNPKAFFDLHDVNGDGHLDINEIESLMQNELSKVYEANNSDDDMQEKYHEMMRMRLEMMKVIDKNKDNLVSKDEFL 330

[Close]

BLAST of Nucleobindin-2 vs. Planmine SMEST
Match: SMESG000022270.1 (SMESG000022270.1)

HSP 1 Score: 161.384 bits (407), Expect = 1.687e-45
Identity = 106/288 (36.81%), Postives = 169/288 (58.68%), Query Frame = 2

Query:  191 LNQKDYAEYMQSVMTELGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLNLAKLELKRTLKRNLNINKYERDKL-IRSLTVDDNNHIHSTDNEKFTKEDFEKLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKD-------PDVKSKRLEELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDINQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKMDTDKDEIVTYEEYL 1030
            LN  DYA Y+  VM+EL  D +F   +     ++S++    ALN  S + R KLDE+KR EI RLN  KLE + + +++   NKY   ++ I  +   D +H+   + E F+ ED ++LI +       +D+  ++ Y+ +E +K  E KK+ E E+         D ++K LEE K        HK +NEPGS EQLK +W+ +D++ E  FN KTFF LHD+N DG+ D+ E+E+++  E+ K Y+  +   D  E+YHE+ +MRK F+E +D DK+ +++ EE++
Sbjct:   39 LNSVDYASYLSEVMSELHKDEKFSKTI---NTAKSSDSVSEALNIVSPNIRTKLDELKRLEIIRLN--KLEKEASKRKS---NKYHPHEMPIPPIKSVDISHLDDINPESFSPEDLKRLIDRTTEIFNQIDKRNDENYKMYEMEK--EAKKRAELERTYSDTKILEDAQNKWLEEQKSKHS---KHKMINEPGSIEQLKSVWQNEDQMSEDSFNPKTFFYLHDVNGDGNLDVAELETLMQKELDKAYDPNDNATDVIERYHEMSRMRKKFLEHVDLDKNHLISLEEFM 313

The following BLAST results are available for this feature:

BLAST of Nucleobindin-2 vs. Ensembl Human
BLAST of Nucleobindin-2 vs. Ensembl Celegans
BLAST of Nucleobindin-2 vs. Ensembl Fly
BLAST of Nucleobindin-2 vs. Ensembl Zebrafish
BLAST of Nucleobindin-2 vs. Ensembl Xenopus
BLAST of Nucleobindin-2 vs. Ensembl Mouse
BLAST of Nucleobindin-2 vs. UniProt/SwissProt
BLAST of Nucleobindin-2 vs. TrEMBL
BLAST of Nucleobindin-2 vs. Ensembl Cavefish
BLAST of Nucleobindin-2 vs. Ensembl Sea Lamprey
BLAST of Nucleobindin-2 vs. Ensembl Yeast
BLAST of Nucleobindin-2 vs. Ensembl Nematostella
BLAST of Nucleobindin-2 vs. Ensembl Medaka
BLAST of Nucleobindin-2 vs. Planmine SMEST

BLAST of Nucleobindin-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
NUCB1	1.193e-33	35.34	nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043]	[more]
NUCB1	1.193e-33	35.34	nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043]	[more]
NUCB1	9.194e-31	33.57	nucleobindin 1 [Source:HGNC Symbol;Acc:HGNC:8043]	[more]
NUCB2	2.560e-30	33.10	nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044]	[more]
NUCB2	6.330e-30	33.10	nucleobindin 2 [Source:HGNC Symbol;Acc:HGNC:8044]	[more]

BLAST of Nucleobindin-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nucb-1	1.945e-28	29.45	NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc...	[more]
nucb-1	2.542e-28	29.45	NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc...	[more]
nucb-1	2.542e-28	29.45	NUCleoBindin homolog [Source:UniProtKB/TrEMBL;Acc...	[more]

BLAST of Nucleobindin-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
NUCB1	7.535e-28	30.82	gene:FBgn0052190 transcript:FBtr0075171	[more]

BLAST of Nucleobindin-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
nucb1	9.920e-32	33.10	nucleobindin 1 [Source:ZFIN;Acc:ZDB-GENE-060825-22...	[more]
nucb1	9.920e-32	33.10	nucleobindin 1 [Source:ZFIN;Acc:ZDB-GENE-060825-22...	[more]
nucb2b	2.341e-31	34.14	nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4...	[more]
nucb2b	2.341e-31	34.14	nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4...	[more]
nucb2a	3.832e-30	33.10	nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-1...	[more]

BLAST of Nucleobindin-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
nucb1	3.305e-33	34.51	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	4.059e-33	34.86	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	5.174e-33	34.86	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	5.174e-33	34.86	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]
nucb1	6.390e-33	34.86	nucleobindin 1 [Source:NCBI gene;Acc:407884]	[more]

BLAST of Nucleobindin-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Nucb1	7.037e-35	35.19	nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388]	[more]
Nucb1	7.369e-35	35.19	nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388]	[more]
Nucb2	9.322e-29	31.67	nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179]	[more]
Nucb2	9.697e-29	31.67	nucleobindin 2 [Source:MGI Symbol;Acc:MGI:1858179]	[more]
Nucb1	4.516e-24	39.74	nucleobindin 1 [Source:MGI Symbol;Acc:MGI:97388]	[more]

BLAST of Nucleobindin-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q0P569\|NUCB1_BOVIN	1.511e-34	35.69	Nucleobindin-1 OS=Bos taurus OX=9913 GN=NUCB1 PE=1...	[more]
sp\|Q63083\|NUCB1_RAT	4.952e-34	35.21	Nucleobindin-1 OS=Rattus norvegicus OX=10116 GN=Nu...	[more]
sp\|Q02819\|NUCB1_MOUSE	5.155e-34	35.19	Nucleobindin-1 OS=Mus musculus OX=10090 GN=Nucb1 P...	[more]
sp\|Q02818\|NUCB1_HUMAN	5.727e-33	35.34	Nucleobindin-1 OS=Homo sapiens OX=9606 GN=NUCB1 PE...	[more]
sp\|Q5R4U1\|NUCB1_PONAB	1.018e-32	35.34	Nucleobindin-1 OS=Pongo abelii OX=9601 GN=NUCB1 PE...	[more]

BLAST of Nucleobindin-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
B7Q350	1.605e-45	35.13	Niemann-Pick type C1 domain-containing protein, pu...	[more]
A0A1I8J719	2.010e-42	36.07	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A267G7U2	2.102e-42	36.07	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A267DJS5	2.408e-42	36.07	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A267G656	3.972e-42	36.07	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]

BLAST of Nucleobindin-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nucb1	4.796e-27	32.75	nucleobindin-2-like [Source:NCBI gene;Acc:10304653...	[more]
nucb1	7.050e-24	32.62	nucleobindin-2-like [Source:NCBI gene;Acc:10304653...	[more]
nucb2a	7.967e-7	24.91	nucleobindin 2a [Source:ZFIN;Acc:ZDB-GENE-030826-1...	[more]

BLAST of Nucleobindin-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
nucb2b	1.067e-18	31.63	nucleobindin 2b [Source:ZFIN;Acc:ZDB-GENE-030131-4...	[more]

BLAST of Nucleobindin-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Nucleobindin-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO43098	1.623e-32	32.63	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Nucleobindin-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
nucb2a	4.230e-31	33.81	nucleobindin-2 [Source:NCBI gene;Acc:101163155]	[more]
NUCB2	4.266e-31	32.51	nucleobindin 2 [Source:NCBI gene;Acc:101162363]	[more]
nucb1	8.302e-30	34.15	nucleobindin-2 [Source:NCBI gene;Acc:101169036]	[more]

BLAST of Nucleobindin-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000017142.1	0.000e+0	99.36	SMESG000017142.1	[more]
SMESG000016720.1	2.150e-116	59.51	SMESG000016720.1	[more]
SMESG000000846.1	8.156e-50	40.21	SMESG000000846.1	[more]
SMESG000000846.1	2.318e-49	41.01	SMESG000000846.1	[more]
SMESG000022270.1	1.687e-45	36.81	SMESG000022270.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30031061 ID=SMED30031061|Name=Nucleobindin-2|organism=Schmidtea mediterranea sexual|type=transcript|length=1226bp
CAATTACTACGTCATTTTGATTGAAAATAAATGCTTGCGTAGTCCTACAC
AAATTTTAAGACATACTTTATTATTCAATCTCTTCTGAATGTTTCATTGT
GATTTATTATTGTTATTATTATTTATATTGTTAATATACACGGATCACGT
TAGAAACGAGGACAAGGAGAAAGTAACATTCTTCAGTGAGTTGAATCAAA
AAGATTATGCAGAGTATATGCAAAGTGTGATGACAGAGTTGGGAACCGAT
ATAGAATTCCAAAATAAAGTTCTTGATAAAAAGGGCTCAAGGTCGAACGA
AGACTACGTTGGAGCTTTAAATTTTGCAAGTAGTTCGGCTCGCAATAAAT
TGGATGAAATAAAACGTAGAGAAATCGAAAGATTAAATTTGGCAAAACTG
GAACTGAAAAGAACATTGAAAAGAAATTTAAATATTAATAAATACGAAAG
AGATAAATTGATACGAAGTTTGACAGTAGACGACAACAATCATATTCATT
CAACAGATAATGAAAAGTTTACAAAAGAAGATTTTGAAAAATTAATTTCT
CAAATTCAGCATGATTTGCGACTTGTCGACCAGGCTAGAGAAAAAGAATA
TCGAAGGCATGAATGGAAAAAAGATTTAGAAATTAAAAAAAAATTGGAAA
ATGAAAAAGATCCCGATGTTAAAAGTAAAAGGTTAGAAGAATTAAAAAAG
GCAAGAAAAACTCCCAGAAATCATAAACCTTTGAATGAACCAGGAAGCGA
GGAACAATTGAAAGAACTGTGGAGAACAGATGATAAACTCGATGAATCAT
ATTTTAATCTTAAAACCTTTTTCAATTTACACGACATCAATCAAGACGGG
CATTGGGATATTGAAGAAGTTGAGTCAATGCTGATTCCCGAAGTTCTAAA
GATTTATGAAGCGGGAAATCAAACTGGAGATCCTGAAGAAAAGTATCACG
AAATAATGAAAATGAGAAAACATTTCATGGAGAAAATGGACACTGATAAA
GATGAAATAGTTACTTACGAAGAATATCTTGAATTTTCAAAAAAGAAAGA
ATTCAAAAACAATAAAGAATGGAAGGTGACTGAAGAGCAAGACAATGAGA
TCTATTCAAATGAAGACATTGAAGAGTTTGAAGAAGAACTAAAAATTGTT
CCAAAAAGGTGACAAATTCAGTAAAATGAAAATTTTCAAGTTCGGATTCT
TGAATGGCTTTTGTTTTATTTTTGTG

protein sequence of SMED30031061-orf-1

>SMED30031061-orf-1 ID=SMED30031061-orf-1|Name=SMED30031061-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=358bp
MFHCDLLLLLLFILLIYTDHVRNEDKEKVTFFSELNQKDYAEYMQSVMTE
LGTDIEFQNKVLDKKGSRSNEDYVGALNFASSSARNKLDEIKRREIERLN
LAKLELKRTLKRNLNINKYERDKLIRSLTVDDNNHIHSTDNEKFTKEDFE
KLISQIQHDLRLVDQAREKEYRRHEWKKDLEIKKKLENEKDPDVKSKRLE
ELKKARKTPRNHKPLNEPGSEEQLKELWRTDDKLDESYFNLKTFFNLHDI
NQDGHWDIEEVESMLIPEVLKIYEAGNQTGDPEEKYHEIMKMRKHFMEKM
DTDKDEIVTYEEYLEFSKKKEFKNNKEWKVTEEQDNEIYSNEDIEEFEEE
LKIVPKR*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000028	Category 2 cell
PLANA:0000035	Category 3 cell
PLANA:0002032	epidermal cell
PLANA:0002111	X2 cell
PLANA:0002142	parenchyma

Vocabulary: **INTERPRO**
Term	Definition
IPR040250	Nucleobindin
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005509	calcium ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 241..316 e-value: 1.0E-8 score: 35.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 288..323 score: 8.544
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 236..271 score: 8.153
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 220..322 e-value: 5.3E-24 score: 86.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 203..223
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..18 score: 0.877
IPR040250	Nucleobindin	PANTHER	PTHR19237	NUCLEOBINDIN	coord: 31..351
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 225..318

Main menu

Nucleobindin-2

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 10

Owlery

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