SMED30031042

Overview
NameSMED30031042
Smed IDSMED30031042
Length (bp)2544
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30031042 (SMED30031042) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30031042 (SMED30031042) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30031042 (SMED30031042) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30031042 (SMED30031042) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30031042

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30031042SMESG000015139.1 SmedASXL_001991SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30031042 SmedASXL_002649SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30031042SMESG000015139.1 dd_Smed_v4_43122_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30031042SMESG000015139.1 dd_Smed_v4_43122_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30031042SMESG000015139.1 dd_Smed_v4_43122_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30031042SMESG000015139.1 dd_Smed_v6_43122_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30031042 dd_Smed_v6_46557_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30031042 vs. TrEMBL
Match: A0A1S3ID45 (G-protein coupled receptor 98-like OS=Lingula unguis OX=7574 GN=LOC106163214 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.692e-14
Identity = 63/253 (24.90%), Postives = 114/253 (45.06%), Query Frame = -1 
Query:  256 VTYVINIVLSPTVLFSNIAPNLNVGCIIISIILYASLTFTCGWPLVYMTAIFNVQAINNVNSKNRYCTYVLVGMGIP-IAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVE-IAEIIYMVICFFIRRLVFICSLNEKSRYKESANQSYNETQGNRNDSIE 1008
            + YV+   +SP +L    A + N  CII+ +  +        W        + +  +N+ ++  +Y  Y L G G+P I      +I   LY KF  ++  + + GDVN +G +CFI +       VI P+++  F + +  + +      W+++DD+Y G  NI E   ++F W    L  ++GGL +     W +  FC    I  +  +V+   +R     C   +K+ Y      S + T G+  D ++
Sbjct:  600 ICYVVAAYISPEILVPPTADD-NSRCIILGLFTHYFFLAQFTWMFTQAINFWKILVLNDEHTDRKYVLYFLTGWGLPAIIVAAFYVITYNLY-KFVYNVPENYIYGDVNLYGEMCFIVNPYAALGGVIGPILVCLFVIGIAFIHAFQVTPQWQAYDDVYRGRYNINEVRTILFFWATIILTWLWGGLHMAYGELWMLIFFCIFNTILGLFALVVYCALRNPCLPCFRPQKASY------SLDITGGSSADGMQ 844

HSP 2 Score: 45.8246 bits (107), Expect = 3.692e-14
Identity = 73/301 (24.25%), Postives = 124/301 (41.20%), Query Frame = -2 
Query: 1041 EHLLNIFCSLSNPLRKDEVVGFSKYLKLLEK-----LVNE--KANQVKCDN--ILKTWSKCPE-NFGL------YFGKIEKPHLKLFMKNVSKFSQIISFETHFLTMSTNPELCCAVELVQLNSDIWIQRKSK-FILASKITVFHITSNRVPSKGSVV-FKIPLTSEMSDSIKIKCGIWIDES---WT-DSYCNVVRYDVISLNDSSSMFCNCSHMSTFSSYVTVDPSPSSDDRLIIY------------ISESLSIVSHLICLMVMLYKKQRNTFFTILFAFYLILGICIRVRQKNINYL 1841
            + +L+IFC L +P R D   G  + +KL E+     L N+  +  +   DN  +L   ++C    FG+      Y    E   LK    NV    Q++         S NP +C  V  ++ +S+ W    +   +LA K+  F I      +  S V F++   +    + + +C ++ +ES   WT  S C       + L   S + C CSH+S+++         +SD  L+ Y               +L+I+SH IC    +Y          +FA  L++ +C       I Y+
Sbjct:  324 KGILSIFCELLDPTRTDSTRGQYELIKLFERFTFICLTNQSCQTPEATDDNKELLMFDNECTYVRFGVARWYPDYINGFEYKGLKQDYFNVPI--QLLDTTAEGGVNSANP-MCQDVHFIEYSSEQWFNPNANNLLLAQKVLTFSIRGRTFGTISSPVKFRVNTDNRRITARRAQC-VYFEESTQAWTSGSVCRATNDLNLDLGIDSFVDCECSHLSSYAVQAQ-----TSDPNLVWYPIWFFISCFIAMTGLALAILSHHIC---SIYS---------MFAANLLMHMCFATMATEICYV 603
[Close]
BLAST of SMED30031042 vs. TrEMBL
Match: A0A267G5X0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029255g1 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 5.436e-14
Identity = 55/207 (26.57%), Postives = 105/207 (50.72%), Query Frame = -1 
Query:  397 YVINIVLSPTVLFSNIAPNLNVGCIIISIILYASLTFTCG--WPLVYMTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSN---SLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVEI 1002
            Y++N  LSP+ +  N     N  CI +S++   +  F C   W L      + V  +N+ ++   Y  +V++G  +P  W  +++  V  Y  F N   +++   ++G VN+ GFIC+IK + +   V+++P++++   L+ + V +   +  W  +DDI  G  N  E   L+  W L     +FGGL +  ++ W + ++  +EI
Sbjct: 5933 YIVNAALSPSSILVNSMAQSNDRCIAVSVL--QTYFFLCQFFWMLAQSVNYWKVLVMNDEHTNRNYHIFVIIGWVVPAVW--VLLCTVISYAVFHNLFPNISPDQLVGSVNNNGFICYIKQLYVMLIVILLPILVVLVGLLFVFVNAYQVLPQWAMYDDICKGRPNHKEVRNLLIFWALLLGCTLFGGLHMAYSDLWWLIIYAVLEI 6135

HSP 2 Score: 48.9062 bits (115), Expect = 5.436e-14
Identity = 48/264 (18.18%), Postives = 99/264 (37.50%), Query Frame = -2 
Query: 1071 NIFCSLSNPLRKDEVVGFSKYLKLLEKLVNEKANQVKCDNILKTWSKCPE----NFGLYFGKIEKPHLKLFMKNVSKFSQ----IISFETHFLTMSTNPELCCAVELVQLNSDIWIQRKSKFILASKITVFHITSNRVPSKGS--VVFK-IPLTSEMSDSIKIKCGIWIDESWTDSYCNVVRYDVISLNDSSSMFCNCSHMSTFSSYVTVDPSPSSDDRLIIYISESLSIVSHLICLMVMLYKKQRNTFFTILFAFYLILGICI 1829
            +++CSL  P R D  V  S   +LL +          C+   +  S CPE       L+   I     K+++   +  S          +  L  ++N   C  V+     S  W +  +  +L   I  F + +  +    S  V F+ I     + D  + +C  W   +W    C V     +       + C+C+H+S+++  +   P+      L  YI   ++   +L  ++   +     + F+     ++++ + +
Sbjct: 5669 SVYCSLIQPARADSTVSRSALAQLLAEFHFAITPASACELTDQQHSACPEIVSRAANLHPRNING---KVYLNTAADLSSNREPYFKIPSKLLPDTSNSSSCQKVQFSVFKSSQWFEVSTLDVLTDTIYSFDLNNKNLDEVISTPVQFRVINFNRRLVDR-RARCAYWSGSNWQSGLCTVDNQAGLDAGQDDFVACSCNHLSSYAVVIDKHPATLVGYNLWFYIPMGIAACVYLFVVIANCFFASNISQFSAHLQLHMLVAVFV 5928
[Close]
BLAST of SMED30031042 vs. TrEMBL
Match: A0A1S3JBB7 (G-protein coupled receptor 98 OS=Lingula unguis OX=7574 GN=LOC106171389 PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.982e-13
Identity = 59/231 (25.54%), Postives = 105/231 (45.45%), Query Frame = -1 
Query:  316 YVINIVLSPTVLFSNIAPNLNVGCIIISIILYASLTFTCGWPLVYMTAIFNVQAINNVNSKNRYCTYVLVGMGIP-IAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVE-IAEIIYMVICFFIRRLVFICSLNEKSRY 1002
            YV+   +SP +L    A + N  CII+ +  +        W        + +  +N+ ++  +Y  Y L G G+P I      +I   LY KF  ++  + + GDVN +G +CFI +       VI P+++  F + +  + +      W+++DD+Y G  NI E   ++F W    L  ++GGL +     W +  FC    I  +  +V+   +R     C   +K+ Y
Sbjct: 3694 YVVAAYISPEILVPPTADD-NSRCIILGLFTHYFFLAQFTWMFTQAINFWKILVLNDEHTDRKYVLYFLTGWGLPAIIVAAFYVITYNLY-KFVYNVPENYIYGDVNLYGEMCFIVNPYAALGGVIGPILVCLFVIGIAFIHAFQVTPQWQAYDDVYRGRYNINEVRTILFFWATIILTWLWGGLHMAYGELWMLIFFCIFNTILGLFALVVYCALRNPCLPCFRPQKASY 3922

HSP 2 Score: 50.8322 bits (120), Expect = 1.982e-13
Identity = 73/299 (24.41%), Postives = 122/299 (40.80%), Query Frame = -2 
Query: 1041 LLNIFCSLSNPLRKDEVVGFSKYLKLLEK-----LVNEKAN--QVKCDN--ILKTWSKCPE-NFGL------YFGKIEKPHLKLFMKNVSKFSQIISFETHFLTMSTNPELCCAVELVQLNSDIWIQ-RKSKFILASKITVFHITSNRVPSKGS-VVFKIPLTSEMSDSIKIKCGIWIDES---WTD-SYCNVVRYDVISLNDSSSMFCNCSHMSTFSSYVTVDPSPSSDDRLIIY------------ISESLSIVSHLICLMVMLYKKQRNTFFTILFAFYLILGICIRVRQKNINYL 1835
            +L+IFC L +P R D   G  + +KL E+     L N+     +   DN  +L   ++C    FG+      Y    E   LK    NV    Q++         S NP  C  V  ++ +S+ W     +  +LA K+  F I      +  S V F++   +    + + +C ++ +ES   WT  S C       + L   S + C CSH+S+++       + +SD  L+ Y               +L+I+SH IC    +Y          +FA  L++ +C       I Y+
Sbjct: 3418 ILSIFCELLDPTRSDSTRGQYELIKLFERFTFVCLTNQSCQTPEATDDNKELLMFDNECTYVRFGVARWYPDYINGFEYKGLKQDYFNVP--IQLLDTTAEGGVNSANPT-CQDVHFIEYSSEQWFNPNANNLLLAQKVLTFSIRGRTFGTISSPVKFRVNTDNRRITARRAQC-VYFEESTQAWTSGSVCRATNDLNLDLGIDSFVDCECSHLSSYAV-----QAQTSDPNLVWYPIWFFISCFIAMTGLALAILSHHIC---SIYS---------MFAANLLMHMCFATMATEICYV 3695
[Close]
BLAST of SMED30031042 vs. TrEMBL
Match: R7VAI0 (G_PROTEIN_RECEP_F2_4 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_219079 PE=4 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.156e-9
Identity = 54/255 (21.18%), Postives = 106/255 (41.57%), Query Frame = -1 
Query:  295 VTYVINIVLSPTVLFSNIAPNLNVGCIIISIILYASLTFTCGWPLVYMTAIFNVQAINNVNSKNRYCTYVLVG----------------MGIPIAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVEIAEIIYMVICFFI-RRLVFICSLNEKSRYKESANQS 1008
            + YVI++ LSP  L S  A   N  C ++ +I +        W +      + V  +N+ +++ +Y  Y  +G                +G+P+    +  I   +  ++ + L    +  DV     ICF+ +     C VI+P ++      +  + +      W+++DD+Y G  NI E   L+  W    +  ++ GL +     W + +FC   +A  +Y  + + + R     C   EKS   E++  +
Sbjct:  794 ICYVIDVYLSPNHLLSIEADQNNYRCTVMGLIFHYFFLTQFSWMMTQAINFYKVLVLNDEHTERKYVLYFFIGWGECEMSHMICAEPSPIGLPVCLLALFYIVTYVIYRYVSFLPEDRIYSDVTQNEDICFVNNAFAALCGVILPCLLFLMVTAIAFIQAFQVTAQWQAYDDVYRGRYNINEIRTLLLFWAFIVITWLWAGLHMAYGQLWMMVLFCIFNVALGVYAFVAYAVLRHPCLPCFRPEKSVAYEASGHA 1048

HSP 2 Score: 33.8834 bits (76), Expect = 2.156e-9
Identity = 68/302 (22.52%), Postives = 120/302 (39.74%), Query Frame = -2 
Query: 1020 LLNIFCSLSNPLRKDE---VVGFSKYLKLLEKLVNEKANQVKCDNIL--KTWSKCPENFGLYFGKIEKPHLKLFMKNVSKFSQIISFETH------------FLTMSTNPELCCAVELVQLNSDIWIQRKSKFI-LASKITVFHITSNRVP-SKGSVVFKIPLTSEMSDSIKIKCGIWID--ESWT--DSYCNVVRYDVISLNDSSSMFCNCSHMSTFSSYVT-VDPSPSSDDR------LIIYISESLSIVSHLICLMVMLYKKQRNTFFTILFAFYLILGICIRVRQKNINYLRCLKVYL 1835
            L+N+ C L +P R +    V G  +  +L+E           C N     T   CP         +E   LK + + ++ F   +S E +            +   S NP  C ++ +V  +S +W   +S  + L  K+    +        +  V +++   ++ + + +  C  + +  E+WT  +  C V     +S++  + + C C HMS ++     V P     D        I  I  +L+IV H ICL          T+ T +FA  L + +C  V    I Y+  + VYL
Sbjct:  525 LMNVLCQLLDPSRSERSGSVRGRWELTRLVEDYAYSLVQHEDCANGAYKATSQDCP--------YLELVVLKDYPEGINDFQWGVSQEDYLKVPSNLLSARDYGRGSNNPR-CYSLAIVVYSSAVWFTSQSTPVFLNDKVLSASLQDQESRFLENPVEYRVYTANKKTAAKRASCRYFDENVETWTSGNDVCTVTNNLALSID--THVDCECKHMSLYAVESNIVRPEIVGFDLWFFVCCFICMIGLALAIVCHHICL----------TYHT-MFAASLHMHMCFAVLAAEICYV--IDVYL 802
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BLAST of SMED30031042 vs. Ensembl Cavefish
Match: adgrv1 (adhesion G protein-coupled receptor V1 [Source:NCBI gene;Acc:415105])

HSP 1 Score: 37.7354 bits (86), Expect = 6.299e-7
Identity = 44/198 (22.22%), Postives = 78/198 (39.39%), Query Frame = -2 
Query: 1017 PELCCAVELVQLNSDIWIQRKSKFILASKITVFHIT---SNRVPSKGSVVFKIPLTSEMSDSIKIKCGIW--IDESWTD--SYCNVVRYDVISLNDSSSMF--CNCSHMSTFSSYVTVDPSPSSDDRL----IIYISE-SLSIVSHLICLMVMLYKKQRNTFFTILFAFYLILGICIRV---RQKNINYLRCLKVYLY 1559
            P  C  V  ++ +++ W        L  K+    I    S  +     VV++I          +  C +W    ESW     +C VV        D +  F  C C+H+S +++Y  +    + ++       I IS  +L+I+SH++C    ++  +     T +    L + IC  V   R K  N   C  + L+
Sbjct: 5684 PSACQKVHFIEYSTEHWFLTNKASALNDKVFSVSIQGRGSRPLADGKEVVYRIHTPDRRVKPRQSLCLLWNQAAESWLSDGQFCRVV--------DDTENFVECACTHLSIYTAYAEIGALSTYNEAFYAAGFICISGFALAIISHVLCSRFPMFAAK---LLTHMMVACLGMQICFLVSAFRGKMFNKDGCAALGLF 5870

HSP 2 Score: 36.1946 bits (82), Expect = 6.299e-7
Identity = 41/192 (21.35%), Postives = 88/192 (45.83%), Query Frame = -1 
Query:  361 GCIIISIILYASLTFTCGWPLVYMTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVEIAEIIYMVICFFI 936
            GC  + +  +        W L+     +    +N+ +++ RY  Y L+  G+P     +VII + + +       + +V G V   G +CFI ++    C      +I    ++V+ + +    + WK++DD+Y G  N +E  ++++L+ L  L+ ++ GL +     W + +     +   +Y+   +FI
Sbjct: 5863 GCAALGLFFHYFHLSQFSWMLIQAINFWQTLVMNDEHTERRYLLYFLLSWGLP----ALVIIVLVIVLLGGYGWNIHEVYGLVQ--GDLCFIPNIYAALCTAAFVPLICLVGVLVVFIHAYHVTQQWKAYDDVYRGRTNSSEVPMVLYLFALVTLVCLWAGLHMAYRYLWMLILLVIFNVFLGLYVFAVYFI 6048
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BLAST of SMED30031042 vs. Ensembl Sea Lamprey
Match: adgrv1 (adhesion G protein-coupled receptor V1 [Source:NCBI gene;Acc:415105])

HSP 1 Score: 51.6026 bits (122), Expect = 9.148e-7
Identity = 68/259 (26.25%), Postives = 107/259 (41.31%), Query Frame = -2 
Query: 1188 QFINRISNDGQDNVYTEHL----LNIFCSLSNPLRKDEVVGFSKYLKLLEKLVNEKANQVKCDNILKTWSKCPENFG--------LYFGKIEKPHLKLFMKNVSKFSQIISFETHFLTMSTNPEL--CCAVELVQLNSDIWIQRKSK-FILASKITVFHITSNRVPSK-----GSVVFKIPLTSEMSDSIKIKCGIW--IDESW-TDS-YCNVVRYDVISLNDSSSMF-CNCSHMSTFSSYVTVDPSPSSDDRLIIYIS 1889
            Q +++I ++G+     E +     NI C L +P R D   G++   ++ E+        ++CD+         ++ G         Y  +I   H       V + +  ++     L + T P L  C  V  V+  S  W Q K +  ++A   TV  +    +PS+     G V ++I  T       K  C +W   DE W  DS YC VV        DS +   C CSHMST++ Y   D   S +  L +YIS
Sbjct: 5587 QVLHQIISEGEKRALNESINKVIYNILCHLVDPSRLD-TRGYTLLGEVTERFAFSLLTGIQCDSAPIRGKTMLDSCGRLMVLSARWYPQQINGHHF------VGRAADSLALPERLLPV-TGPSLQECYDVLFVEYRSQQWYQPKDQPSVMAG--TVLSVGVKGIPSRPLTQEGEVTYRIHATDRRVVPHKSACLLWNPADEGWLADSKYCRVVE-------DSFNFVECACSHMSTYAVYGKTDNLTSYN--LAVYIS 5826
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BLAST of SMED30031042 vs. Ensembl Nematostella
Match: EDO42266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1V5])

HSP 1 Score: 54.299 bits (129), Expect = 2.273e-7
Identity = 53/216 (24.54%), Postives = 99/216 (45.83%), Query Frame = -1 
Query:  355 YVINIVLSPTVLFSNIAPNLNVGCIIISIILYASLTFTCGWPLVYMTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVEIAEIIYMVICFFIRR 1002
            YVI   +S T    N +P   V C ++ + ++        W  V    ++ +  +N+ ++  +Y  + L+G G+P+    +      +  + + S   +D+ GDV+  G +CFI +        + PV++    + V+   +      W+ +DDIY G  NI E   +I L+    +  +F GL +     W I VF  ++I   +Y+ I +FI R
Sbjct: 5066 YVIGAYVSSTQAVVNESPA--VRCSVLGVFIHYFYLCQFSWMFVQGWNLWRILVLNDEHTDRKYVLFFLLGWGMPVVVIVLY----IVVAQAAFSWPFTDMYGDVHQNGDMCFIPNAYAALASAVAPVLLFLMGVAVVFTQAYQVTMQWRYYDDIYRGHYNIKEVRHVIALFVFVTITWLFAGLHLAYGYLWMIIVFAILDILLGLYVFILYFILR 5275
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BLAST of SMED30031042 vs. Planmine SMEST
Match: SMESG000015139.1 (SMESG000015139.1)

HSP 1 Score: 466.077 bits (1198), Expect = 2.443e-159
Identity = 278/280 (99.29%), Postives = 280/280 (100.00%), Query Frame = -1 
Query:   28 MTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDXXXXXXXXXXXXXXXXXXXXXXXXISLCRIRTWKSFDDIYLGSRNITEXXXXXXXXXXXXXLMVFGGLTIVTNNRWTIFVFCAVEIAEIIYMVICFFIRRLVFICSLNEKSRYKESANQSYNETQGNRNDSIEDLIYYIKFGDENSKIQETEFIPQVTDSESVVTFEDVDGSSMALSTKDLDNNMSFLNAFDDIHSSTSDKEYIMESQI 867
            MTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDVIIGTCVVIIPVVIIFFWLIVLIVISLCRIRTWKSFDDIYLGSRNITEFILLIFLWFLNFLLMVFGGLTIVTNNRWTIFVFCAVEIAEIIYMVICFFIRRLVF+CSLNEKSRYKESANQSYNETQGNRNDSIEDLIYYIKFGDENSKIQETEFIPQVTDSESVVTFEDVDGSSMALSTKDLDNNMSFLNAFDDI+SSTSDKEYIMESQI
Sbjct:    1 MTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSNSLTVSDVIGDVNSFGFICFIKDVIIGTCVVIIPVVIIFFWLIVLIVISLCRIRTWKSFDDIYLGSRNITEFILLIFLWFLNFLLMVFGGLTIVTNNRWTIFVFCAVEIAEIIYMVICFFIRRLVFVCSLNEKSRYKESANQSYNETQGNRNDSIEDLIYYIKFGDENSKIQETEFIPQVTDSESVVTFEDVDGSSMALSTKDLDNNMSFLNAFDDIYSSTSDKEYIMESQI 280
The following BLAST results are available for this feature:
BLAST of SMED30031042 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
A0A1S3ID453.692e-1424.90G-protein coupled receptor 98-like OS=Lingula ungu... [more]
A0A267G5X05.436e-1426.57Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1S3JBB71.982e-1325.54G-protein coupled receptor 98 OS=Lingula unguis OX... [more]
R7VAI02.156e-921.18G_PROTEIN_RECEP_F2_4 domain-containing protein OS=... [more]
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BLAST of SMED30031042 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
adgrv16.299e-722.22adhesion G protein-coupled receptor V1 [Source:NCB... [more]
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BLAST of SMED30031042 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
adgrv19.148e-726.25adhesion G protein-coupled receptor V1 [Source:NCB... [more]
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BLAST of SMED30031042 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO422662.273e-724.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30031042 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30031042 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000015139.12.443e-15999.29SMESG000015139.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031042 ID=SMED30031042|Name=SMED30031042|organism=Schmidtea mediterranea sexual|type=transcript|length=2544bp
AAAATTCTTGGAGTAACCTTTGACTTATATTTGCGATTCCATAATATATT
CTTTATCGCTTGTTGAAGAATGAATATCATCAAATGCGTTTAGGAAACTC
ATATTATTATCCAAATCTTTCGTTGATAGCGCCATTGATGATCCGTCCAC
ATCTTCAAAGGTGACAACACTTTCACTGTCAGTGACTTGAGGAATAAATT
CGGTTTCTTGGATTTTTGAATTTTCATCACCAAATTTTATATAGTAGATC
AAATCTTCAATCGAATCATTACGATTTCCTTGAGTTTCATTGTAAGATTG
GTTCGCGGATTCCTTGTACCGAGATTTTTCGTTTAATGAGCAGATAAAGA
CAAGACGTCGAATAAAGAAACAAATCACCATATAAATTATCTCCGCTATT
TCGACAGCACAAAATACAAATATCGTCCATCGGTTGTTCGTAACAATTGT
CAGTCCACCGAAAACCATAAGTAAAAAATTCAGAAACCATAAAAATATCA
ACAAGATAAACTCTGTAATATTTCTCGATCCCAAATATATATCATCAAAG
GATTTCCAAGTTCGAATCCGGCAGAGTGAAATAACGATGAGCACTATCAG
CCAGAAAAATATTATTACAACGGGAATTATTACAACGCATGTACCGATAA
TGACGTCTTTAATAAAACATATGAAACCAAAACTATTAACATCGCCGATT
ACATCCGACACCGTCAAACTGTTGGAAAATTTAACGTATAGAGTGACGCC
TATGATAACAATGGTTGTCCATGCAATGGGAATTCCCATCCCAACTAAAA
CATATGTGCAATATCTATTTTTAGAATTGACATTATTGATAGCTTGCACG
TTGAAAATGGCAGTCATGTATACTAAAGGCCAACCACACGTAAACGTTAG
AGAAGCATATAATATAATGCTTATGATTATGCAACCAACGTTCAAATTAG
GGGCGATATTTGAAAAAAGTACAGTTGGACTTAAAACAATATTTATAACG
TAAGTTACCTAGAGTTATAGAGATAAACTTTAAGGCAACGCAAGTAATTT
ATATTTTTTTGCCTTACTCTTATGCATATACCTAATATCAAATAAAAGGC
GAACAAAATTGTAAAAAATGTATTTCTCTGTTTTTTGTATAACATTACCA
TTAAACATATCAAATGAGATACTATAGATAAAGATTCACTAATATAAATA
ATTAAACGATCATCACTCGATGGGGAGGGGTCAACTGTGACATAGCTACT
AAATGTAGACATGTGGGAACAGTTGCAAAACATGCTTGACGAATCATTTA
GGGAGATAACATCATACCTTACAACGTTACAGTAACTGTCAGTCCATGAT
TCATCTATCCAAATACCACATTTTATTTTAATGGAATCAGACATTTCTGA
AGTCAAAGGAATTTTGAACACTACCGAACCCTTACTTGGAACTCTGTTAC
TGGTAATGTGGAAAACTGTTATTTTAGATGCAAGAATAAACTTTGATTTT
CTTTGAATCCAAATATCGCTGTTTAATTGAACCAATTCTACCGCGCAACA
AAGTTCAGGATTTGTTGACATGGTCAGGAAATGAGTTTCAAATGAAATAA
TTTGACTGAACTTTGATACGTTTTTCATAAATAATTTCAAATGAGGTTTT
TCAATTTTTCCGAAATACAAACCGAAATTTTCTGGACATTTGGACCATGT
CTTCAAAATATTGTCACATTTCACTTGATTAGCCTTTTCATTAACAAGCT
TTTCCAGAAGCTTTAAATATTTGGAAAATCCGACAACTTCATCTTTGCGC
AAGGGATTCGATAAGGAACAGAATATGTTTAATAAATGTTCTGTATATAC
ATTATCCTGACCATCATTGGAAATTCGATTAATAAATTGTTCTAACATTT
CTCCAATAATGACATTATGATAAACTGTTAGCACGGACCTTTCTATAAGC
CTCTCCAGAATCACAAGAGAATTTATTAAGCTTTTTACATCGAAAGAACT
TAAAAATTTATTTTTTACAAATTCTAATAAAGTTTCTAGAAAACATGCCA
AGCTTCCTGACGAGGTGCTATCTTGTAGAAATAAGACCTGAGAGGAATTG
TTCATAAACTGTAGTGCAGTTGAGGAAATTTCACTACAGAACAAGAAGTC
ACGAGGAAGCCATCCATCCTTTATAAATTCGTTTGAAAGCATATTTAATT
TCAGGGTTGAAAGGACGTGTGACAGGTTTGAGACAAATTTAAAATCACTT
TTTCTTACAAGGCTTCTCGCGGTTATATTTGATTCTCTGTTATTTTCATA
AAGATCTTTTTTTAAGTTTTGTGTGAAAGTTTTGTCTAATTTATCGAATT
TATTCAGAAAAAACCGGTTATTCTGTTCCAGAACTTGTGTGGAAAAACTG
GTAAAGTTTGATAAAACATTTGCATATTTATTCACTAATAGAAAAAATAT
AAATAAAGCCATCCGCAATAAAATGGAATAAAATGTTCAGTAACTGAAAG
CTATTAATTATTTATGTAATCTTTTCTCAAAAATCAATTCAGTA
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protein sequence of SMED30031042-orf-1

>SMED30031042-orf-1 ID=SMED30031042-orf-1|Name=SMED30031042-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=281bp
MTAIFNVQAINNVNSKNRYCTYVLVGMGIPIAWTTIVIIGVTLYVKFSNS
LTVSDVIGDVNSFGFICFIKDVIIGTCVVIIPVVIIFFWLIVLIVISLCR
IRTWKSFDDIYLGSRNITEFILLIFLWFLNFLLMVFGGLTIVTNNRWTIF
VFCAVEIAEIIYMVICFFIRRLVFICSLNEKSRYKESANQSYNETQGNRN
DSIEDLIYYIKFGDENSKIQETEFIPQVTDSESVVTFEDVDGSSMALSTK
DLDNNMSFLNAFDDIHSSTSDKEYIMESQI*
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protein sequence of SMED30031042-orf-2

>SMED30031042-orf-2 ID=SMED30031042-orf-2|Name=SMED30031042-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp
MALFIFFLLVNKYANVLSNFTSFSTQVLEQNNRFFLNKFDKLDKTFTQNL
KKDLYENNRESNITARSLVRKSDFKFVSNLSHVLSTLKLNMLSNEFIKDG
WLPRDFLFCSEISSTALQFMNNSSQVLFLQDSTSSGSLACFLETLLEFVK
NKFLSSFDVKSLINSLVILERLIERSVLTVYHNVIIGEMLEQFINRISND
GQDNVYTEHLLNIFCSLSNPLRKDEVVGFSKYLKLLEKLVNEKANQVKCD
NILKTWSKCPENFGLYFGKIEKPHLKLFMKNVSKFSQIISFETHFLTMST
NPELCCAVELVQLNSDIWIQRKSKFILASKITVFHITSNRVPSKGSVVFK
IPLTSEMSDSIKIKCGIWIDESWTDSYCNVVRYDVISLNDSSSMFCNCSH
MSTFSSYVTVDPSPSSDDRLIIYISESLSIVSHLICLMVMLYKKQRNTFF
TILFAFYLILGICIRVRQKNINYLRCLKVYLYNSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000203GPS
Vocabulary: cellular component
TermDefinition
GO:0016020membrane