Rho-associated protein kinase

Overview
NameRho-associated protein kinase
Smed IDSMED30031032
Length (bp)4011
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rho-associated protein kinase (SMED30031032) t-SNE clustered cells

Violin plots show distribution of expression levels for Rho-associated protein kinase (SMED30031032) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rho-associated protein kinase (SMED30031032) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rho-associated protein kinase (SMED30031032) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30031032

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30031032h1SMcG0015497 dd_Smed_v4_11429_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
anterior region of the whole animalSMED30031032h1SMcG0015497 dd_Smed_v4_11429_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031032h1SMcG0015497 Contig38286newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031032h1SMcG0015497 Contig38286uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031032h1SMcG0015497 Contig9002newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30031032h1SMcG0015497 Contig9002uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
muscle cellSMED30031032h1SMcG0015497 dd_Smed_v4_11429_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Rho-associated protein kinase vs. Ensembl Human
Match: ROCK1 (Rho associated coiled-coil containing protein kinase 1 [Source:HGNC Symbol;Acc:HGNC:10251])

HSP 1 Score: 393.66 bits (1010), Expect = 2.683e-116
Identity = 183/400 (45.75%), Postives = 264/400 (66.00%), Query Frame = 3
Query:   75 NVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            N++ D K  ++ D L D    L  D      L  NK   NF  ++K    K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL+FP D+++S  A + +   + DR VRLG+     +IK H+FF N   +W W+T++D+ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:   16 NLLRDPKSEVNSDCLLDGLDALVYDL-DFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-EGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRN-GVEEIKRHLFFKND--QWAWETLRDTVAPVVPDLSSDIDTSNFDDLEED----------KGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. Ensembl Human
Match: ROCK1 (Rho associated coiled-coil containing protein kinase 1 [Source:HGNC Symbol;Acc:HGNC:10251])

HSP 1 Score: 390.578 bits (1002), Expect = 9.611e-114
Identity = 183/400 (45.75%), Postives = 264/400 (66.00%), Query Frame = 3
Query:   75 NVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            N++ D K  ++ D L D    L  D      L  NK   NF  ++K    K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL+FP D+++S  A + +   + DR VRLG+     +IK H+FF N   +W W+T++D+ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:   16 NLLRDPKSEVNSDCLLDGLDALVYDL-DFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-EGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRN-GVEEIKRHLFFKND--QWAWETLRDTVAPVVPDLSSDIDTSNFDDLEED----------KGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. Ensembl Human
Match: ROCK2 (Rho associated coiled-coil containing protein kinase 2 [Source:HGNC Symbol;Acc:HGNC:10252])

HSP 1 Score: 365.925 bits (938), Expect = 1.024e-113
Identity = 181/405 (44.69%), Postives = 271/405 (66.91%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +I+ H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNG-VEEIRQHPFFKND--QWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYYR 417          
BLAST of Rho-associated protein kinase vs. Ensembl Human
Match: ROCK2 (Rho associated coiled-coil containing protein kinase 2 [Source:HGNC Symbol;Acc:HGNC:10252])

HSP 1 Score: 388.652 bits (997), Expect = 6.466e-113
Identity = 181/405 (44.69%), Postives = 271/405 (66.91%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +I+ H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRN-GVEEIRQHPFFKND--QWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYYR 417          
BLAST of Rho-associated protein kinase vs. Ensembl Human
Match: CDC42BPB (CDC42 binding protein kinase beta [Source:HGNC Symbol;Acc:HGNC:1738])

HSP 1 Score: 336.65 bits (862), Expect = 3.056e-94
Identity = 177/434 (40.78%), Postives = 268/434 (61.75%), Query Frame = 3
Query:   51 VLLENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVC-FYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNT-KYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPD-DEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDV--DTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDK 1337
            +LL+  ++N     +  +S++ L D    L+ +C S S L  +KY   F +  K   + + + ++   DF ++K+IG+GAFG+V +V+ K T  ++A+KILNK +++    T  ++EERD+++  D +WI  LHYAFQD   LY+VMDY  GG+L+  +SK E  L +D+  FY  E+VLAI+ +H   +VHRDIKPDN+L+D+ GHI+LADFGSC+KM+     V  + A+GTPDYISPE+L +  D   KYG E DWW+LGV +YE+ YGE PFY+++L+ TYGKIM HE+   FP    +VS  A D +++LIC R  RLGQ     D K H FF        W+ I++  +P+IP + +  DTS FD  +DDV  +T  L   +     T F G ++ F GF++   ++   +     +++ SN + +D+
Sbjct:   13 LLLDGPWRN-----ESALSVETLLDVLVCLYTEC-SHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMND-DGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQN-GIEDFKKHAFFEG----LNWENIRNLEAPYIPDVSSPSDTSNFDV-DDDVLRNTEILPPGSH----TGFSGLHLPFIGFTFTT-ESCFSDRGSLKSIMQSNTLTKDE 428          
BLAST of Rho-associated protein kinase vs. Ensembl Celegans
Match: let-502 (Rho-associated protein kinase let-502 [Source:UniProtKB/Swiss-Prot;Acc:P92199])

HSP 1 Score: 383.645 bits (984), Expect = 6.057e-113
Identity = 183/400 (45.75%), Postives = 265/400 (66.25%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            ++  ++ + D K  I+++ L D+   L  DCK +  L   K   NF  ++++V E L   RMK +DF  LK+IG+GAFG+V LVRH +T  ++A+K+LNK  ++    + F+ EERDIM   +S WIV+L YAFQDP+ LYMVM+Y+PGG+LVN M+  EV  EK   FY AEIV A+  LH+ G++HRD+KPDN+LI I GHIKLADFG+CVKM+     V C+ A+GTPDYISPEVL +Q  + ++GKEVDWW++GV +YE+  GE PFY++ L++TY  IM H+ SL FP +  +S  A D +KK +     RLG+     DI++H FF N   EWT+ T+++++ P IP L++D DT++F    ++++T   + +       TF G  + F GF+Y
Sbjct:    3 QDELRDQLVDPKSPINIESLLDTITALVNDCK-IPVLMRMKSVDNFISRYERVVESLAALRMKAADFRQLKVIGRGAFGEVHLVRHTRTNTVYAMKMLNKDDMIKRADSAFFWEERDIMAHANSEWIVRLQYAFQDPRHLYMVMEYMPGGDLVNLMTSYEV-SEKWTRFYTAEIVEALAALHSMGYIHRDVKPDNMLISISGHIKLADFGTCVKMN-ANGVVRCSTAVGTPDYISPEVLRNQGQDAEFGKEVDWWSVGVFIYEMLVGETPFYAEALVSTYTNIMNHKTSLKFPDEPLISTQAKDIIKKFLSAAPDRLGRN-SVDDIRNHKFFVND--EWTFATLREASPPVIPSLKSDDDTTHF----EEIETRDRDNAGDFQLPKTFNGNQLPFIGFTY 392          
BLAST of Rho-associated protein kinase vs. Ensembl Celegans
Match: mrck-1 (Serine/threonine-protein kinase mrck-1 [Source:UniProtKB/Swiss-Prot;Acc:O01583])

HSP 1 Score: 352.443 bits (903), Expect = 4.548e-100
Identity = 184/409 (44.99%), Postives = 256/409 (62.59%), Query Frame = 3
Query:   57 LENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSK-VEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDN-TKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDE----VSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFG--EDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            LEN + +  S   E +S + L DS   L+ +C + S L   K    F +  K V  K    R+   DF +LK+IG+GAFG+V +VR +  G+++A+KILNK ++V    T  ++EERD+++  D RWI  LHYAFQD K LY VMDY  GG+++  +SK V+ + E    FY AE+VLAI+ LH  G+VHRD+KPDN+L+D++GHI+LADFGSC+++      V  N A+GTPDYISPE+L +  D   +YGKE DWW+LG+ +YE+ YG  PFYS+ L++TYGKIM+H+  L F PDDE    VS  A D +++LIC   VR G+    SD + H FF        W+TI+DS  P++P + +  DTS FD    EDD  T  L ++        F G ++ F GFSY
Sbjct:   17 LENIYMDGPSKKPEALSFETLIDSLICLYDECCN-STLRKEKCIAEFVESVKTVISKAKKLRLSRDDFEVLKVIGKGAFGEVAVVRMRGVGEIYAMKILNKWEMVKRAETACFREERDVLVYGDRRWITNLHYAFQDEKNLYFVMDYYIGGDMLTLLSKFVDHIPESMAKFYIAEMVLAIDSLHRLGYVHRDVKPDNVLLDMQGHIRLADFGSCLRI-LADGSVASNVAVGTPDYISPEILRAMEDGRGRYGKECDWWSLGICMYEMLYGTTPFYSERLVDTYGKIMSHQDMLDF-PDDEIDWVVSEEAKDLIRQLICSSDVRFGRN-GLSDFQLHPFFEGID----WNTIRDSNPPYVPEVSSPEDTSNFDVDVCEDDF-TPCLQETQPPRVLAAFTGNHLPFVGFSY 416          
BLAST of Rho-associated protein kinase vs. Ensembl Celegans
Match: mrck-1 (Serine/threonine-protein kinase mrck-1 [Source:UniProtKB/Swiss-Prot;Acc:O01583])

HSP 1 Score: 352.443 bits (903), Expect = 4.548e-100
Identity = 184/409 (44.99%), Postives = 256/409 (62.59%), Query Frame = 3
Query:   57 LENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSK-VEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDN-TKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDE----VSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFG--EDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            LEN + +  S   E +S + L DS   L+ +C + S L   K    F +  K V  K    R+   DF +LK+IG+GAFG+V +VR +  G+++A+KILNK ++V    T  ++EERD+++  D RWI  LHYAFQD K LY VMDY  GG+++  +SK V+ + E    FY AE+VLAI+ LH  G+VHRD+KPDN+L+D++GHI+LADFGSC+++      V  N A+GTPDYISPE+L +  D   +YGKE DWW+LG+ +YE+ YG  PFYS+ L++TYGKIM+H+  L F PDDE    VS  A D +++LIC   VR G+    SD + H FF        W+TI+DS  P++P + +  DTS FD    EDD  T  L ++        F G ++ F GFSY
Sbjct:   17 LENIYMDGPSKKPEALSFETLIDSLICLYDECCN-STLRKEKCIAEFVESVKTVISKAKKLRLSRDDFEVLKVIGKGAFGEVAVVRMRGVGEIYAMKILNKWEMVKRAETACFREERDVLVYGDRRWITNLHYAFQDEKNLYFVMDYYIGGDMLTLLSKFVDHIPESMAKFYIAEMVLAIDSLHRLGYVHRDVKPDNVLLDMQGHIRLADFGSCLRI-LADGSVASNVAVGTPDYISPEILRAMEDGRGRYGKECDWWSLGICMYEMLYGTTPFYSERLVDTYGKIMSHQDMLDF-PDDEIDWVVSEEAKDLIRQLICSSDVRFGRN-GLSDFQLHPFFEGID----WNTIRDSNPPYVPEVSSPEDTSNFDVDVCEDDF-TPCLQETQPPRVLAAFTGNHLPFVGFSY 416          
BLAST of Rho-associated protein kinase vs. Ensembl Celegans
Match: mrck-1 (Serine/threonine-protein kinase mrck-1 [Source:UniProtKB/Swiss-Prot;Acc:O01583])

HSP 1 Score: 349.747 bits (896), Expect = 3.193e-99
Identity = 182/408 (44.61%), Postives = 255/408 (62.50%), Query Frame = 3
Query:   57 LENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSK-VEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDN-TKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDE----VSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKS-TTFIGENIQFSGFSY 1259
            LEN + +  S   E +S + L DS   L+ +C + S L   K    F +  K V  K    R+   DF +LK+IG+GAFG+V +VR +  G+++A+KILNK ++V    T  ++EERD+++  D RWI  LHYAFQD K LY VMDY  GG+++  +SK V+ + E    FY AE+VLAI+ LH  G+VHRD+KPDN+L+D++GHI+LADFGSC+++      V  N A+GTPDYISPE+L +  D   +YGKE DWW+LG+ +YE+ YG  PFYS+ L++TYGKIM+H+  L F PDDE    VS  A D +++LIC   VR G+    SD + H FF        W+TI+DS  P++P + +  DTS FD   D  + +F     Q  +    F G ++ F GFSY
Sbjct:   17 LENIYMDGPSKKPEALSFETLIDSLICLYDECCN-STLRKEKCIAEFVESVKTVISKAKKLRLSRDDFEVLKVIGKGAFGEVAVVRMRGVGEIYAMKILNKWEMVKRAETACFREERDVLVYGDRRWITNLHYAFQDEKNLYFVMDYYIGGDMLTLLSKFVDHIPESMAKFYIAEMVLAIDSLHRLGYVHRDVKPDNVLLDMQGHIRLADFGSCLRI-LADGSVASNVAVGTPDYISPEILRAMEDGRGRYGKECDWWSLGICMYEMLYGTTPFYSERLVDTYGKIMSHQDMLDF-PDDEIDWVVSEEAKDLIRQLICSSDVRFGRN-GLSDFQLHPFFEGID----WNTIRDSNPPYVPEVSSPEDTSNFDV--DVCEDDFTPCETQPPRVLAAFTGNHLPFVGFSY 414          
BLAST of Rho-associated protein kinase vs. Ensembl Celegans
Match: mrck-1 (Serine/threonine-protein kinase mrck-1 [Source:UniProtKB/Swiss-Prot;Acc:O01583])

HSP 1 Score: 349.747 bits (896), Expect = 3.193e-99
Identity = 182/408 (44.61%), Postives = 255/408 (62.50%), Query Frame = 3
Query:   57 LENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSK-VEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDN-TKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDE----VSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKS-TTFIGENIQFSGFSY 1259
            LEN + +  S   E +S + L DS   L+ +C + S L   K    F +  K V  K    R+   DF +LK+IG+GAFG+V +VR +  G+++A+KILNK ++V    T  ++EERD+++  D RWI  LHYAFQD K LY VMDY  GG+++  +SK V+ + E    FY AE+VLAI+ LH  G+VHRD+KPDN+L+D++GHI+LADFGSC+++      V  N A+GTPDYISPE+L +  D   +YGKE DWW+LG+ +YE+ YG  PFYS+ L++TYGKIM+H+  L F PDDE    VS  A D +++LIC   VR G+    SD + H FF        W+TI+DS  P++P + +  DTS FD   D  + +F     Q  +    F G ++ F GFSY
Sbjct:   17 LENIYMDGPSKKPEALSFETLIDSLICLYDECCN-STLRKEKCIAEFVESVKTVISKAKKLRLSRDDFEVLKVIGKGAFGEVAVVRMRGVGEIYAMKILNKWEMVKRAETACFREERDVLVYGDRRWITNLHYAFQDEKNLYFVMDYYIGGDMLTLLSKFVDHIPESMAKFYIAEMVLAIDSLHRLGYVHRDVKPDNVLLDMQGHIRLADFGSCLRI-LADGSVASNVAVGTPDYISPEILRAMEDGRGRYGKECDWWSLGICMYEMLYGTTPFYSERLVDTYGKIMSHQDMLDF-PDDEIDWVVSEEAKDLIRQLICSSDVRFGRN-GLSDFQLHPFFEGID----WNTIRDSNPPYVPEVSSPEDTSNFDV--DVCEDDFTPCETQPPRVLAAFTGNHLPFVGFSY 414          
BLAST of Rho-associated protein kinase vs. Ensembl Fly
Match: Rok (gene:FBgn0026181 transcript:FBtr0340152)

HSP 1 Score: 366.311 bits (939), Expect = 1.534e-105
Identity = 175/399 (43.86%), Postives = 257/399 (64.41%), Query Frame = 3
Query:   63 NNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            N  +  + D     ++D L D+   L  DC   S L   K    +  ++K +  ++   RM   DF+ +K+IG GAFG+VQLVRHK +  ++A+K L+K +++    + F+ EER IM   +S WIV+LH+AFQD K LYMVMD++PGG++V+ M   ++  EK   FY  E+VLA++ +HN GFVHRD+KPDN+L+D  GH+KLADFG+C++M    + V+ N A+GTPDYISPEVL SQ  + +YG+E DWW++G+ LYE+ +GE PFY+D+L+ TYGKIM H+ SLSFPP+ E+S  A   ++  + DR+ RLG+     DIK+H FF N +  W++D I++S  P +P L +D DT  F+  E D     +    +G     F G ++ F GF+Y
Sbjct:   23 NTLEREMRDPTSICNVDCLLDTVSALVSDCDHES-LRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIP-EKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSN-AVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRY-GIEDIKAHPFFRNDT--WSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKG-----FDGNHLPFIGFTY 410          

HSP 2 Score: 51.2174 bits (121), Expect = 9.258e-6
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 2949 NVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH-----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            N +N+ +   W++++V +  + + FY  D+   +       L LS +  VRSV+  DVI A + EIPRIFQ+ Y
Sbjct: 1144 NKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLY 1217          
BLAST of Rho-associated protein kinase vs. Ensembl Fly
Match: Rok (gene:FBgn0026181 transcript:FBtr0340153)

HSP 1 Score: 366.696 bits (940), Expect = 1.596e-105
Identity = 175/399 (43.86%), Postives = 257/399 (64.41%), Query Frame = 3
Query:   63 NNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            N  +  + D     ++D L D+   L  DC   S L   K    +  ++K +  ++   RM   DF+ +K+IG GAFG+VQLVRHK +  ++A+K L+K +++    + F+ EER IM   +S WIV+LH+AFQD K LYMVMD++PGG++V+ M   ++  EK   FY  E+VLA++ +HN GFVHRD+KPDN+L+D  GH+KLADFG+C++M    + V+ N A+GTPDYISPEVL SQ  + +YG+E DWW++G+ LYE+ +GE PFY+D+L+ TYGKIM H+ SLSFPP+ E+S  A   ++  + DR+ RLG+     DIK+H FF N +  W++D I++S  P +P L +D DT  F+  E D     +    +G     F G ++ F GF+Y
Sbjct:   14 NTLEREMRDPTSICNVDCLLDTVSALVSDCDHES-LRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIP-EKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSN-AVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRY-GIEDIKAHPFFRNDT--WSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKG-----FDGNHLPFIGFTY 401          

HSP 2 Score: 51.2174 bits (121), Expect = 9.188e-6
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 2949 NVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH-----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            N +N+ +   W++++V +  + + FY  D+   +       L LS +  VRSV+  DVI A + EIPRIFQ+ Y
Sbjct: 1135 NKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLY 1208          
BLAST of Rho-associated protein kinase vs. Ensembl Fly
Match: Rok (gene:FBgn0026181 transcript:FBtr0340151)

HSP 1 Score: 366.311 bits (939), Expect = 1.674e-105
Identity = 175/399 (43.86%), Postives = 257/399 (64.41%), Query Frame = 3
Query:   63 NNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            N  +  + D     ++D L D+   L  DC   S L   K    +  ++K +  ++   RM   DF+ +K+IG GAFG+VQLVRHK +  ++A+K L+K +++    + F+ EER IM   +S WIV+LH+AFQD K LYMVMD++PGG++V+ M   ++  EK   FY  E+VLA++ +HN GFVHRD+KPDN+L+D  GH+KLADFG+C++M    + V+ N A+GTPDYISPEVL SQ  + +YG+E DWW++G+ LYE+ +GE PFY+D+L+ TYGKIM H+ SLSFPP+ E+S  A   ++  + DR+ RLG+     DIK+H FF N +  W++D I++S  P +P L +D DT  F+  E D     +    +G     F G ++ F GF+Y
Sbjct:   23 NTLEREMRDPTSICNVDCLLDTVSALVSDCDHES-LRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIP-EKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSN-AVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRY-GIEDIKAHPFFRNDT--WSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKG-----FDGNHLPFIGFTY 410          

HSP 2 Score: 51.2174 bits (121), Expect = 9.345e-6
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 2949 NVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH-----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            N +N+ +   W++++V +  + + FY  D+   +       L LS +  VRSV+  DVI A + EIPRIFQ+ Y
Sbjct: 1135 NKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLY 1208          
BLAST of Rho-associated protein kinase vs. Ensembl Fly
Match: Rok (gene:FBgn0026181 transcript:FBtr0074286)

HSP 1 Score: 366.696 bits (940), Expect = 1.679e-105
Identity = 175/399 (43.86%), Postives = 257/399 (64.41%), Query Frame = 3
Query:   63 NNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            N  +  + D     ++D L D+   L  DC   S L   K    +  ++K +  ++   RM   DF+ +K+IG GAFG+VQLVRHK +  ++A+K L+K +++    + F+ EER IM   +S WIV+LH+AFQD K LYMVMD++PGG++V+ M   ++  EK   FY  E+VLA++ +HN GFVHRD+KPDN+L+D  GH+KLADFG+C++M    + V+ N A+GTPDYISPEVL SQ  + +YG+E DWW++G+ LYE+ +GE PFY+D+L+ TYGKIM H+ SLSFPP+ E+S  A   ++  + DR+ RLG+     DIK+H FF N +  W++D I++S  P +P L +D DT  F+  E D     +    +G     F G ++ F GF+Y
Sbjct:   23 NTLEREMRDPTSICNVDCLLDTVSALVSDCDHES-LRRLKNIEQYAAKYKPLAMQINQLRMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIP-EKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQVVSSN-AVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRY-GIEDIKAHPFFRNDT--WSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKG-----FDGNHLPFIGFTY 410          

HSP 2 Score: 51.2174 bits (121), Expect = 9.198e-6
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 2949 NVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH-----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            N +N+ +   W++++V +  + + FY  D+   +       L LS +  VRSV+  DVI A + EIPRIFQ+ Y
Sbjct: 1144 NKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRADAKEIPRIFQLLY 1217          
BLAST of Rho-associated protein kinase vs. Ensembl Fly
Match: gek (gene:FBgn0023081 transcript:FBtr0072287)

HSP 1 Score: 310.457 bits (794), Expect = 4.004e-86
Identity = 166/391 (42.46%), Postives = 241/391 (61.64%), Query Frame = 3
Query:  105 SLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVC-FYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNT-KYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDD----EVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            SLD L D+F  L+ +C S S+L   K  ++F K  K     +   R+   DF++LKIIG+GAFG+V +V+   T  ++A+KILNK +++    T  ++EERD+++  D +WI  LHYAFQD   LY+VMDY  GG+L+  +SK E  L +D+  FY  E++LAIN +H   +VHRDIKPDN+L+D +GH++LADFGSC+++D     V  N A+GTPDYISPE+L +  D   +YG E DWW+LGV +YE+ YGE PFY+++L+ TYGKIM H+   + P  +    +VS  + D + KLIC    RLGQ     D   H +F        W  I+   +P++P + +  DTS FD  ++DV    L  S     +  F G ++ F GF++
Sbjct:   50 SLDYLLDTFIVLYDEC-SNSSLRREKGVSDFLKLSKPFVHIVRKLRLSRDDFDILKIIGRGAFGEVCVVQMISTEKVYAMKILNKWEMLKRAETACFREERDVLVFGDRQWITNLHYAFQDNINLYLVMDYYCGGDLLTLLSKFEDKLPEDMAKFYITEMILAINSIHQIRYVHRDIKPDNVLLDKRGHVRLADFGSCLRLDK-DGTVQSNVAVGTPDYISPEILRAMEDGKGRYGTECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHQNCFNLPSQETLNYKVSETSQDLLCKLICIPENRLGQN-GIQDFMDHPWFVG----IDWKNIRQGPAPYVPEVSSPTDTSNFDVDDNDVR---LTDSIPPSANPAFSGFHLPFIGFTF 430          
BLAST of Rho-associated protein kinase vs. Ensembl Zebrafish
Match: rock1 (Rho-associated, coiled-coil containing protein kinase 1 [Source:ZFIN;Acc:ZDB-GENE-100712-2])

HSP 1 Score: 385.185 bits (988), Expect = 4.925e-112
Identity = 182/399 (45.61%), Postives = 261/399 (65.41%), Query Frame = 3
Query:   78 VVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            ++ D K  ++ D L DS   L  D      L  NK   +F K++K    K+ + RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A   +   + DR VRLG+     +IK H FF N   +WTW+ I+++ +P +P L  D DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:   19 MLKDPKSEVNTDCLLDSLDALVYDL-DFPALRKNKSIDDFLKRYKDTISKIRELRMKAEDYEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSNWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-DGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPEDSDISKDAKSLICAFLTDREVRLGRN-GVDEIKRHGFFKND--QWTWENIRETAAPVVPELSCDTDTSNFDDIEED----------RGEEETFPIPKAFVGNQLPFVGFTY 401          
BLAST of Rho-associated protein kinase vs. Ensembl Zebrafish
Match: rock2a (rho-associated, coiled-coil containing protein kinase 2a [Source:ZFIN;Acc:ZDB-GENE-030115-3])

HSP 1 Score: 381.333 bits (978), Expect = 1.599e-110
Identity = 193/434 (44.47%), Postives = 282/434 (64.98%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDD---VDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTNREN 1361
             + ++ D +  I+L+ L DS   L LD      L  NK   NF  +++KV   + + +M+  DF+ +K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   DS W+V+L  AFQD + LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV +YE+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S    + +   + DR VRLG++    +IK H FF N   +WT+ TI+++ +P +P L +D+DTS FD  E+D   V+T F            F+G  + F GF+Y       KEN+   N +NS+ +  D   S NRE+
Sbjct:   16 LEAMIKDPRSAINLESLLDSMNALVLDL-DFPALRKNKNIENFLNRYEKVMNHIRELQMRPEDFDRVKVIGRGAFGEVQLVRHKASQKVYAMKVLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRSLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDRYGHLKLADFGTCMKMDG-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIYEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISQEGKNIICAFLTDREVRLGRS-GVEEIKRHPFFRND--QWTFSTIRETAAPVVPELSSDIDTSNFDEIEEDKGEVET-FPT-------PKAFVGNQLPFVGFTYF------KENQLL-NAVNSSAMKNDHPVS-NRED 427          

HSP 2 Score: 56.6102 bits (135), Expect = 2.367e-7
Identity = 37/129 (28.68%), Postives = 59/129 (45.74%), Query Frame = 3
Query: 2868 STNLQNRNSKTESMTMDFRNINQILEVN--------VKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICYDKNDSLYLTAQMNPISKSTTS 3218
            S N+Q+ +S + S   D      +LE+         V+N  K+  WE+++V +  + + FY  +    H      L +  L  VRSV+  DV  A + EIPRIFQI Y          ++ P+    +S
Sbjct: 1115 SLNVQSLDSASMSSGPDMDTDESLLEIRLEGWLSLPVRNNTKRFGWERKYVVVSSKKILFYNSEQDKEHSNPYMVLDIDKLFHVRSVTQTDVYRADAKEIPRIFQILYANEGESKKEQELEPLPGDKSS 1243          
BLAST of Rho-associated protein kinase vs. Ensembl Zebrafish
Match: rock2a (rho-associated, coiled-coil containing protein kinase 2a [Source:ZFIN;Acc:ZDB-GENE-030115-3])

HSP 1 Score: 380.948 bits (977), Expect = 1.691e-110
Identity = 190/431 (44.08%), Postives = 279/431 (64.73%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTNREN 1361
             + ++ D +  I+L+ L DS   L LD      L  NK   NF  +++KV   + + +M+  DF+ +K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   DS W+V+L  AFQD + LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV +YE+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S    + +   + DR VRLG++    +IK H FF N   +WT+ TI+++ +P +P L +D+DTS FD  E+D       +         F+G  + F GF+Y       KEN+   N +NS+ +  D   S NRE+
Sbjct:   16 LEAMIKDPRSAINLESLLDSMNALVLDL-DFPALRKNKNIENFLNRYEKVMNHIRELQMRPEDFDRVKVIGRGAFGEVQLVRHKASQKVYAMKVLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRSLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDRYGHLKLADFGTCMKMDG-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIYEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISQEGKNIICAFLTDREVRLGRS-GVEEIKRHPFFRND--QWTFSTIRETAAPVVPELSSDIDTSNFDEIEEDK-----GEVETFPTPKAFVGNQLPFVGFTYF------KENQLL-NAVNSSAMKNDHPVS-NRED 427          

HSP 2 Score: 56.6102 bits (135), Expect = 2.400e-7
Identity = 37/129 (28.68%), Postives = 59/129 (45.74%), Query Frame = 3
Query: 2868 STNLQNRNSKTESMTMDFRNINQILEVN--------VKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICYDKNDSLYLTAQMNPISKSTTS 3218
            S N+Q+ +S + S   D      +LE+         V+N  K+  WE+++V +  + + FY  +    H      L +  L  VRSV+  DV  A + EIPRIFQI Y          ++ P+    +S
Sbjct: 1115 SLNVQSLDSASMSSGPDMDTDESLLEIRLEGWLSLPVRNNTKRFGWERKYVVVSSKKILFYNSEQDKEHSNPYMVLDIDKLFHVRSVTQTDVYRADAKEIPRIFQILYANEGESKKEQELEPLPGDKSS 1243          
BLAST of Rho-associated protein kinase vs. Ensembl Zebrafish
Match: rock2b (rho-associated, coiled-coil containing protein kinase 2b [Source:ZFIN;Acc:ZDB-GENE-060125-2])

HSP 1 Score: 373.244 bits (957), Expect = 1.158e-107
Identity = 181/399 (45.36%), Postives = 261/399 (65.41%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ + K  ++L+ L DS   L LD    + L  NK    F  +++K    L + ++K  DF+ +K+IG+GAFG VQLVRHK +  ++A+K L+K ++V    + F+ EERDIM    S WIV+L  AFQD K LY+VM+++PGG+LV   S  ++  E+   FY AE+VLA++ +H+ GF+HRDIKPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL+SQ     YG+E DWW++GV +YE+  G+ PFYS++L+ TYGKIM H+ SL+FP D E+S  A D +   +  R VRLG+T    +IK H FF N   +WT+DTI+D+ +P +P L +D+DTS FD   DD+  + +  +        F G  + F GF+Y R
Sbjct:   16 LETLIKNQKSPLNLESLLDSVTALALDLNHPA-LRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIP-EEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDS-TGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRT-GVDEIKCHPFFKND--QWTFDTIRDTMAPVVPELSSDIDTSNFD---DDIKDDPIG-TETFPPPRAFAGNQLPFVGFTYFR 404          
BLAST of Rho-associated protein kinase vs. Ensembl Zebrafish
Match: rock2b (rho-associated, coiled-coil containing protein kinase 2b [Source:ZFIN;Acc:ZDB-GENE-060125-2])

HSP 1 Score: 373.244 bits (957), Expect = 1.238e-107
Identity = 181/399 (45.36%), Postives = 261/399 (65.41%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ + K  ++L+ L DS   L LD    + L  NK    F  +++K    L + ++K  DF+ +K+IG+GAFG VQLVRHK +  ++A+K L+K ++V    + F+ EERDIM    S WIV+L  AFQD K LY+VM+++PGG+LV   S  ++  E+   FY AE+VLA++ +H+ GF+HRDIKPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL+SQ     YG+E DWW++GV +YE+  G+ PFYS++L+ TYGKIM H+ SL+FP D E+S  A D +   +  R VRLG+T    +IK H FF N   +WT+DTI+D+ +P +P L +D+DTS FD   DD+  + +  +        F G  + F GF+Y R
Sbjct:   16 LETLIKNQKSPLNLESLLDSVTALALDLNHPA-LRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIP-EEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDS-TGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRT-GVDEIKCHPFFKND--QWTFDTIRDTMAPVVPELSSDIDTSNFD---DDIKDDPIG-TETFPPPRAFAGNQLPFVGFTYFR 404          
BLAST of Rho-associated protein kinase vs. Ensembl Xenopus
Match: med18 (mediator complex subunit 18 [Source:Xenbase;Acc:XB-GENE-1002158])

HSP 1 Score: 383.645 bits (984), Expect = 3.783e-111
Identity = 182/401 (45.39%), Postives = 263/401 (65.59%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVD--TNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ D +  I+++ L D   ++ LD      L  NK   NF  +++K+  ++   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD K LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA+N +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     DIK H FF N   +W WD I+++ +P +P L +D+DTS FD  EDD      F N          F G  + F GF+Y R
Sbjct:   16 LEAILRDPRSPINVEGLLDGLNSIVLDL-DFPALRKNKNIDNFLNRYEKIVREVRKLQMKAEDYDVVKVIGRGAFGEVQLVRHKGSQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDEKHLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALNAIHSMGLIHRDVKPDNMLLDKYGHLKLADFGTCMKMDQ-TGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPEDVEISVHAKNLICAFLTDREVRLGRN-GIEDIKQHPFFKND--QWNWDNIRETVAPVVPELASDIDTSNFDDIEDDKGDAETFPN-------PKAFAGNQLPFIGFTYYR 403          
BLAST of Rho-associated protein kinase vs. Ensembl Xenopus
Match: med18 (mediator complex subunit 18 [Source:Xenbase;Acc:XB-GENE-1002158])

HSP 1 Score: 383.259 bits (983), Expect = 8.709e-111
Identity = 182/401 (45.39%), Postives = 263/401 (65.59%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVD--TNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ D +  I+++ L D   ++ LD      L  NK   NF  +++K+  ++   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD K LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA+N +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     DIK H FF N   +W WD I+++ +P +P L +D+DTS FD  EDD      F N          F G  + F GF+Y R
Sbjct:   16 LEAILRDPRSPINVEGLLDGLNSIVLDL-DFPALRKNKNIDNFLNRYEKIVREVRKLQMKAEDYDVVKVIGRGAFGEVQLVRHKGSQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDEKHLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALNAIHSMGLIHRDVKPDNMLLDKYGHLKLADFGTCMKMDQ-TGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPEDVEISVHAKNLICAFLTDREVRLGRN-GIEDIKQHPFFKND--QWNWDNIRETVAPVVPELASDIDTSNFDDIEDDKGDAETFPN-------PKAFAGNQLPFIGFTYYR 403          
BLAST of Rho-associated protein kinase vs. Ensembl Xenopus
Match: med18 (mediator complex subunit 18 [Source:Xenbase;Acc:XB-GENE-1002158])

HSP 1 Score: 382.874 bits (982), Expect = 1.735e-110
Identity = 182/401 (45.39%), Postives = 263/401 (65.59%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVD--TNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ D +  I+++ L D   ++ LD      L  NK   NF  +++K+  ++   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD K LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA+N +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     DIK H FF N   +W WD I+++ +P +P L +D+DTS FD  EDD      F N          F G  + F GF+Y R
Sbjct:   16 LEAILRDPRSPINVEGLLDGLNSIVLDL-DFPALRKNKNIDNFLNRYEKIVREVRKLQMKAEDYDVVKVIGRGAFGEVQLVRHKGSQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDEKHLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALNAIHSMGLIHRDVKPDNMLLDKYGHLKLADFGTCMKMDQ-TGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPEDVEISVHAKNLICAFLTDREVRLGRN-GIEDIKQHPFFKND--QWNWDNIRETVAPVVPELASDIDTSNFDDIEDDKGDAETFPN-------PKAFAGNQLPFIGFTYYR 403          
BLAST of Rho-associated protein kinase vs. Ensembl Xenopus
Match: med18 (mediator complex subunit 18 [Source:Xenbase;Acc:XB-GENE-1002158])

HSP 1 Score: 379.793 bits (974), Expect = 5.384e-110
Identity = 186/412 (45.15%), Postives = 266/412 (64.56%), Query Frame = 3
Query:   36 KLNNYVLLENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVD--TNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            K+ NY++   +  + +SD   +I LDL F +             L  NK   NF  +++K+  ++   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD K LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA+N +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     DIK H FF N   +W WD I+++ +P +P L +D+DTS FD  EDD      F N          F G  + F GF+Y R
Sbjct:    8 KILNYLIKMESMGDGLSDGLNSIVLDLDFPA-------------LRKNKNIDNFLNRYEKIVREVRKLQMKAEDYDVVKVIGRGAFGEVQLVRHKGSQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDEKHLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALNAIHSMGLIHRDVKPDNMLLDKYGHLKLADFGTCMKMDQ-TGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPEDVEISVHAKNLICAFLTDREVRLGRN-GIEDIKQHPFFKND--QWNWDNIRETVAPVVPELASDIDTSNFDDIEDDKGDAETFPN-------PKAFAGNQLPFIGFTYYR 394          
BLAST of Rho-associated protein kinase vs. Ensembl Xenopus
Match: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1 [Source:NCBI gene;Acc:100496057])

HSP 1 Score: 377.096 bits (967), Expect = 2.962e-109
Identity = 171/365 (46.85%), Postives = 248/365 (67.95%), Query Frame = 3
Query:   75 NVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDD 1169
             ++ D K  ++ D L D    +  D      +  NK   NF  ++K    K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +W W+T++D+ +P +P L +D+DTS FD  E+D
Sbjct:   16 GILRDPKSEVNTDCLLDGLDAMVYDL-DFPAMRKNKNIDNFLNRYKDTMSKVRDLRMKADDYEVVKVIGRGAFGEVQLVRHKSSRKVYAMKLLSKLEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNR-EGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPEDSDISKEAKNLICAFLTDREVRLGRN-GVEEIKRHPFFKND--QWAWETLRDTVAPVVPDLTSDIDTSNFDDLEED 374          
BLAST of Rho-associated protein kinase vs. Ensembl Mouse
Match: Rock1 (Rho-associated coiled-coil containing protein kinase 1 [Source:MGI Symbol;Acc:MGI:107927])

HSP 1 Score: 373.629 bits (958), Expect = 4.730e-116
Identity = 187/421 (44.42%), Postives = 272/421 (64.61%), Query Frame = 3
Query:   75 NVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNI 1322
            N++ D K  ++ D L D    L  D      L  NK   NF  ++K    K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL+FP D+++S  A + +   + DR VRLG+     +IK H+FF N   +W W+T++D+ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y   +        S+N  +SN+
Sbjct:   16 NLLRDPKSEVNSDCLLDGLDALVYDL-DFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-EGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNG-VEEIKRHLFFKND--QWAWETLRDTVAPVVPDLSSDIDTSNFDDLEED----------KGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANASENRSSSNV 420          
BLAST of Rho-associated protein kinase vs. Ensembl Mouse
Match: Rock1 (Rho-associated coiled-coil containing protein kinase 1 [Source:MGI Symbol;Acc:MGI:107927])

HSP 1 Score: 390.193 bits (1001), Expect = 8.023e-114
Identity = 187/421 (44.42%), Postives = 272/421 (64.61%), Query Frame = 3
Query:   75 NVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNI 1322
            N++ D K  ++ D L D    L  D      L  NK   NF  ++K    K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL+FP D+++S  A + +   + DR VRLG+     +IK H+FF N   +W W+T++D+ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y   +        S+N  +SN+
Sbjct:   16 NLLRDPKSEVNSDCLLDGLDALVYDL-DFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-EGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRN-GVEEIKRHLFFKND--QWAWETLRDTVAPVVPDLSSDIDTSNFDDLEED----------KGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANASENRSSSNV 420          
BLAST of Rho-associated protein kinase vs. Ensembl Mouse
Match: Rock2 (Rho-associated coiled-coil containing protein kinase 2 [Source:MGI Symbol;Acc:MGI:107926])

HSP 1 Score: 385.185 bits (988), Expect = 1.439e-111
Identity = 180/405 (44.44%), Postives = 270/405 (66.67%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +IK H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYFR 417          

HSP 2 Score: 51.6026 bits (122), Expect = 9.462e-6
Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 3
Query: 2892 SKTESMTMDFRNINQILEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDV---QTCHFRLL-LSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            S +E ++ + R +   L + V+N  KK  W K++V +  + + FY  +    Q+  + +L +  L  VR V+  DV  A + EIPRIFQI Y
Sbjct: 1199 SSSEPISTESR-LEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILY 1289          
BLAST of Rho-associated protein kinase vs. Ensembl Mouse
Match: Rock2 (Rho-associated coiled-coil containing protein kinase 2 [Source:MGI Symbol;Acc:MGI:107926])

HSP 1 Score: 384.415 bits (986), Expect = 1.589e-111
Identity = 180/405 (44.44%), Postives = 270/405 (66.67%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +IK H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYFR 417          
BLAST of Rho-associated protein kinase vs. Ensembl Mouse
Match: Rock1 (Rho-associated coiled-coil containing protein kinase 1 [Source:MGI Symbol;Acc:MGI:107927])

HSP 1 Score: 370.933 bits (951), Expect = 8.189e-109
Identity = 166/344 (48.26%), Postives = 240/344 (69.77%), Query Frame = 3
Query:  243 MKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            MK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL+FP D+++S  A + +   + DR VRLG+     +IK H+FF N   +W W+T++D+ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:    1 MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVP-EKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-EGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRN-GVEEIKRHLFFKND--QWAWETLRDTVAPVVPDLSSDIDTSNFDDLEED----------KGDEETFPIPKAFVGNQLPFVGFTY 329          
BLAST of Rho-associated protein kinase vs. UniProt/SwissProt
Match: sp|O75116|ROCK2_HUMAN (Rho-associated protein kinase 2 OS=Homo sapiens OX=9606 GN=ROCK2 PE=1 SV=4)

HSP 1 Score: 388.267 bits (996), Expect = 4.366e-112
Identity = 181/405 (44.69%), Postives = 271/405 (66.91%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +I+ H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRN-GVEEIRQHPFFKND--QWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYYR 417          
BLAST of Rho-associated protein kinase vs. UniProt/SwissProt
Match: sp|A8WVU9|ROCK_CAEBR (Rho-associated protein kinase let-502 OS=Caenorhabditis briggsae OX=6238 GN=let-502 PE=3 SV=2)

HSP 1 Score: 384.8 bits (987), Expect = 4.720e-112
Identity = 181/391 (46.29%), Postives = 263/391 (67.26%), Query Frame = 3
Query:   87 DDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            D K  I+++ L D+   L  DCK +  L   K   NF  +++++ E +   RMK +DF  LK+IG+GAFG+V LVRH +T  ++A+K+LNK  ++    + F+ EERDIM   +S WIV+L YAFQDP+ LYMVM+Y+PGG+LVN M+  EV  EK   FY AEIV A+  LHN G++HRD+KPDN+LI   GHIKLADFG+CVKM+     V C+ A+GTPDYISPEVL +Q  ++++GKEVDWW++GV +YE+  GE PFY++ L++TY  IM H+ SL FP +  +S  A D +KK +     RLG+  +  +I++H FF N   EWT++T++D+T P +P L++D DT++F    ++++T   + ++      TF G  + F GF+Y
Sbjct:   12 DPKSPINIESLLDTITALVNDCK-IPVLMRMKSVDNFISRYERIVESVAALRMKATDFRQLKVIGRGAFGEVHLVRHTRTNTVYAMKMLNKDDMIKRADSAFFWEERDIMAHANSEWIVRLQYAFQDPRHLYMVMEYMPGGDLVNLMTSYEV-SEKWTRFYTAEIVEALAALHNMGYIHRDVKPDNMLISRSGHIKLADFGTCVKMNS-NGVVRCSTAVGTPDYISPEVLRNQGKDSEFGKEVDWWSVGVFIYEMLVGETPFYAEALVSTYANIMNHQTSLRFPDEPLISTQAKDIIKKFLSAAPERLGKN-NVDEIRNHKFFKND--EWTFETLKDATPPIVPSLKSDDDTTHF----EEIETRDRDNASDFQLPKTFNGNQLPFIGFTY 392          
BLAST of Rho-associated protein kinase vs. UniProt/SwissProt
Match: sp|P92199|ROCK_CAEEL (Rho-associated protein kinase let-502 OS=Caenorhabditis elegans OX=6239 GN=let-502 PE=1 SV=1)

HSP 1 Score: 383.645 bits (984), Expect = 7.705e-112
Identity = 183/400 (45.75%), Postives = 265/400 (66.25%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            ++  ++ + D K  I+++ L D+   L  DCK +  L   K   NF  ++++V E L   RMK +DF  LK+IG+GAFG+V LVRH +T  ++A+K+LNK  ++    + F+ EERDIM   +S WIV+L YAFQDP+ LYMVM+Y+PGG+LVN M+  EV  EK   FY AEIV A+  LH+ G++HRD+KPDN+LI I GHIKLADFG+CVKM+     V C+ A+GTPDYISPEVL +Q  + ++GKEVDWW++GV +YE+  GE PFY++ L++TY  IM H+ SL FP +  +S  A D +KK +     RLG+     DI++H FF N   EWT+ T+++++ P IP L++D DT++F    ++++T   + +       TF G  + F GF+Y
Sbjct:    3 QDELRDQLVDPKSPINIESLLDTITALVNDCK-IPVLMRMKSVDNFISRYERVVESLAALRMKAADFRQLKVIGRGAFGEVHLVRHTRTNTVYAMKMLNKDDMIKRADSAFFWEERDIMAHANSEWIVRLQYAFQDPRHLYMVMEYMPGGDLVNLMTSYEV-SEKWTRFYTAEIVEALAALHSMGYIHRDVKPDNMLISISGHIKLADFGTCVKMN-ANGVVRCSTAVGTPDYISPEVLRNQGQDAEFGKEVDWWSVGVFIYEMLVGETPFYAEALVSTYTNIMNHKTSLKFPDEPLISTQAKDIIKKFLSAAPDRLGRN-SVDDIRNHKFFVND--EWTFATLREASPPVIPSLKSDDDTTHF----EEIETRDRDNAGDFQLPKTFNGNQLPFIGFTY 392          
BLAST of Rho-associated protein kinase vs. UniProt/SwissProt
Match: sp|M3TYT0|ROCK2_PIG (Rho-associated protein kinase 2 OS=Sus scrofa OX=9823 GN=ROCK2 PE=1 SV=1)

HSP 1 Score: 386.341 bits (991), Expect = 2.466e-111
Identity = 181/405 (44.69%), Postives = 270/405 (66.67%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD K LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +IK H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYYR 417          
BLAST of Rho-associated protein kinase vs. UniProt/SwissProt
Match: sp|P70336|ROCK2_MOUSE (Rho-associated protein kinase 2 OS=Mus musculus OX=10090 GN=Rock2 PE=1 SV=1)

HSP 1 Score: 385.956 bits (990), Expect = 3.333e-111
Identity = 180/405 (44.44%), Postives = 270/405 (66.67%), Query Frame = 3
Query:   60 ENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +   + ++ D +  I+++ L D   +L LD      L  NK   NF  +++K+ +K+   +MK  D++++K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L  AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G +HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ SL FP D E+S  A + +   + DR VRLG+     +IK H FF N   +W WD I+++ +P +P L +D+D+S FD  ED   DV+T  + ++        F+G  + F GF+Y R
Sbjct:   27 QRKLEALIRDPRSPINVESLLDGLNSLVLDL-DFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDE-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKA--------FVGNQLPFIGFTYFR 417          
BLAST of Rho-associated protein kinase vs. TrEMBL
Match: A0A3Q1HNI3 (Rho-associated, coiled-coil containing protein kinase 1 OS=Anabas testudineus OX=64144 PE=4 SV=1)

HSP 1 Score: 396.741 bits (1018), Expect = 1.563e-114
Identity = 185/409 (45.23%), Postives = 267/409 (65.28%), Query Frame = 3
Query:   57 LENNFQNV---VSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            +E  F+ +   + D K  ++ D L D    L  D      L  NK   NF  ++K+   K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L +AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +WTW+ I+D+ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:    7 MEARFEKIDAMLKDPKSEVNTDCLLDGLDALVYDL-DFPALRKNKSIDNFLNRYKETISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSSWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMDK-DGMVRCDTAVGTPDYISPEVLKSQGGDGNYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRN-GVDEIKRHPFFKND--QWTWENIRDTAAPVVPELASDIDTSNFDDIEED----------RGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. TrEMBL
Match: A0A1S3JNX4 (rho-associated protein kinase 2-like isoform X2 OS=Lingula unguis OX=7574 GN=LOC106174872 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.947e-114
Identity = 189/410 (46.10%), Postives = 270/410 (65.85%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKS 1298
             +  + D    IS+D L DS  +L  DC     +  NK   NF  +++K+   + + RMK  DF ++K+IG+GAFG+VQLVRH  T  ++A+K+L+K +++    + F+ EER+IM   +S+WIV+LH+AFQD + LYMVMDY+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GFVHRD+KPDN+L+D +GH+KLADFG+C++MD     V  + A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TYGKIM H+ SLSFP D E+S  A   ++  + DRS RLGQ +   +IK H FF  +  +WTWD+I+ +  P +P L  D+DTS F    DD++ +   Q    +    F G ++ F GF+Y R  +   E K S
Sbjct:   17 LEGRMKDPNSEISVDGLLDSITSLVADC-DFPAIRRNKNVENFLGRYEKIVADVNNSRMKADDFEMVKVIGRGAFGEVQLVRHVSTRKVYAMKLLSKYEMIKRSDSAFFWEEREIMANANSQWIVQLHFAFQDHRFLYMVMDYMPGGDLVNLMSNYDV-PEKWAKFYCAEVVLALDAIHSMGFVHRDVKPDNMLLDAQGHLKLADFGTCMRMDK-DGMVRSDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYGKIMDHKNSLSFPEDIEISRDAKSLIRAFLTDRSERLGQ-RGVEEIKGHPFFTRND-QWTWDSIRKTVPPVVPELSCDVDTSNF----DDIEPDETAQETFPVPK-AFAGNHLPFIGFTYNRAYHLLSEAKAS 416          
BLAST of Rho-associated protein kinase vs. TrEMBL
Match: A0A0S7LGB8 (ROCK1 OS=Poeciliopsis prolifica OX=188132 GN=ROCK1 PE=4 SV=1)

HSP 1 Score: 379.793 bits (974), Expect = 5.353e-114
Identity = 187/409 (45.72%), Postives = 267/409 (65.28%), Query Frame = 3
Query:   57 LENNFQNV---VSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            +E  F+ +   + D K  I+ D L D    L  D      L  NK   NF  ++KK   K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L +AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  N  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +WTWD I+++ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:    7 MEARFEKIDAMLQDPKSEINTDCLLDGLDALVYDL-DFPALRKNKSIDNFLNRYKKTISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNE-DGMVQCDTAVGTPDYISPEVLKSQGGNGNYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRN-GVDEIKRHPFFKND--QWTWDNIRETAAPVVPELSSDIDTSNFDDIEED----------RGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. TrEMBL
Match: A0A0S7LGC5 (ROCK1 OS=Poeciliopsis prolifica OX=188132 GN=ROCK1 PE=4 SV=1)

HSP 1 Score: 377.481 bits (968), Expect = 1.011e-113
Identity = 187/409 (45.72%), Postives = 267/409 (65.28%), Query Frame = 3
Query:   57 LENNFQNV---VSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            +E  F+ +   + D K  I+ D L D    L  D      L  NK   NF  ++KK   K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L +AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  N  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +WTWD I+++ +P +P L +D+DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:    7 MEARFEKIDAMLQDPKSEINTDCLLDGLDALVYDL-DFPALRKNKSIDNFLNRYKKTISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNE-DGMVQCDTAVGTPDYISPEVLKSQGGNGNYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRN-GVDEIKRHPFFKND--QWTWDNIRETAAPVVPELSSDIDTSNFDDIEED----------RGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. TrEMBL
Match: A0A0S7LGC9 (ROCK1 OS=Poeciliopsis prolifica OX=188132 GN=ROCK1 PE=3 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 7.647e-113
Identity = 185/404 (45.79%), Postives = 264/404 (65.35%), Query Frame = 3
Query:   57 LENNFQNV---VSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            +E  F+ +   + D K  I+ D L D    L  D      L  NK   NF  ++KK   K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L +AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  N  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +WTWD I+++ +P +P L +D+DTS FD  E+D       +         F+G  + F GF+Y
Sbjct:    7 MEARFEKIDAMLQDPKSEINTDCLLDGLDALVYDL-DFPALRKNKSIDNFLNRYKKTISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNE-DGMVQCDTAVGTPDYISPEVLKSQGGNGNYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRNG-VDEIKRHPFFKND--QWTWDNIRETAAPVVPELSSDIDTSNFDDIEED-----RGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. Ensembl Cavefish
Match: rock2a (rho-associated protein kinase 2 [Source:NCBI gene;Acc:103042784])

HSP 1 Score: 381.719 bits (979), Expect = 7.389e-111
Identity = 180/400 (45.00%), Postives = 264/400 (66.00%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD-FGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ D +  I+L+ L D+   L LD      L  NK    F  +++KV   + + +MK  DF+ +K+IG+GAFG+VQLVRH+ +  ++A+K+L+K +++    + F+ EERDIM   DS W+V+L  AFQD + LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+V+A++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV ++E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     +IKSH FF N   +WT+DTI+D+ +P +P L +D+DTS FD   ED  D               F+G  + F GF+Y +
Sbjct:   16 LEAMIRDPRSAINLESLLDAVNALVLDL-DFPALRKNKNIETFLNRYEKVMNHIRELQMKPEDFDRVKVIGRGAFGEVQLVRHRASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRYLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRNGHLKLADFGTCMKMDS-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISKEAKNIICAFLTDREVRLGRN-GVGEIKSHPFFKND--QWTFDTIRDTAAPVVPELSSDIDTSNFDEIEEDKGDVETFPTPK------AFVGNQLPFVGFTYFK 403          

HSP 2 Score: 55.4546 bits (132), Expect = 4.815e-7
Identity = 28/76 (36.84%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L + V+N  K+  WE+++V +  + + FY  +    H      L +  L  VRSV+  DV  A + EIPRIFQI Y
Sbjct: 1143 LSLPVRNNTKRFGWERKYVVVSSKKILFYNSEQDKEHSNPYMVLDIDKLFHVRSVTQTDVYRADAKEIPRIFQILY 1218          
BLAST of Rho-associated protein kinase vs. Ensembl Cavefish
Match: ROCK1 (Rho associated coiled-coil containing protein kinase 1 [Source:HGNC Symbol;Acc:HGNC:10251])

HSP 1 Score: 354.369 bits (908), Expect = 2.539e-110
Identity = 175/364 (48.08%), Postives = 246/364 (67.58%), Query Frame = 3
Query:  168 LPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            L  NK   NF  + + V  K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H  GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A   +   + DR VRLG+     +IK H FF N   +WTWD I+D+ +P +P L +D+DTS FD  E+D       +         F+G  + F GF+Y
Sbjct:   31 LRKNKSIDNFLSRCECV--KIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSSWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHAMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNK-DGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPEDSDISKDAKSLICAFLTDREVRLGRNG-VDEIKRHSFFKND--QWTWDNIRDTAAPVVPELSSDIDTSNFDDIEED-----RGEEETFPIPKAFVGNQLPFVGFTY 382          
BLAST of Rho-associated protein kinase vs. Ensembl Cavefish
Match: rock2a (rho-associated protein kinase 2 [Source:NCBI gene;Acc:103042784])

HSP 1 Score: 380.563 bits (976), Expect = 5.041e-110
Identity = 180/400 (45.00%), Postives = 264/400 (66.00%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD-FGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             + ++ D +  I+L+ L D+   L LD      L  NK    F  +++KV   + + +MK  DF+ +K+IG+GAFG+VQLVRH+ +  ++A+K+L+K +++    + F+ EERDIM   DS W+V+L  AFQD + LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+V+A++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV ++E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     +IKSH FF N   +WT+DTI+D+ +P +P L +D+DTS FD   ED  D               F+G  + F GF+Y +
Sbjct:   16 LEAMIRDPRSAINLESLLDAVNALVLDL-DFPALRKNKNIETFLNRYEKVMNHIRELQMKPEDFDRVKVIGRGAFGEVQLVRHRASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRYLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRNGHLKLADFGTCMKMDS-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISKEAKNIICAFLTDREVRLGRN-GVGEIKSHPFFKND--QWTFDTIRDTAAPVVPELSSDIDTSNFDEIEEDKGDVETFPTPK------AFVGNQLPFVGFTYFK 403          

HSP 2 Score: 55.0694 bits (131), Expect = 6.219e-7
Identity = 28/76 (36.84%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L + V+N  K+  WE+++V +  + + FY  +    H      L +  L  VRSV+  DV  A + EIPRIFQI Y
Sbjct: 1143 LSLPVRNNTKRFGWERKYVVVSSKKILFYNSEQDKEHSNPYMVLDIDKLFHVRSVTQTDVYRADAKEIPRIFQILY 1218          
BLAST of Rho-associated protein kinase vs. Ensembl Cavefish
Match: rock2b (rho-associated, coiled-coil containing protein kinase 2b [Source:ZFIN;Acc:ZDB-GENE-060125-2])

HSP 1 Score: 367.081 bits (941), Expect = 3.884e-106
Identity = 185/434 (42.63%), Postives = 276/434 (63.59%), Query Frame = 3
Query:   63 NNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTNR 1355
               + ++ + +  ++L+ L DS   L LD    + L  NK    F  +++K   +L + + +  DF+ +K+IG+GA+G VQLVRHK +  ++A+K L+K +++    + F+ EERDIM   +S WIV+L  AFQD + LY+VM+++PGG+LV      + + EK   FY AE+V+A++ +H+ GF+HRDIKPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL+SQ     YG+E DWW++GV LYE+  G+ PFY+++L+ TYGKIM H+ SL+FP D E+S  A   +   + DR VRLG+T    +IK H FF N   +WT+DTI+D+ +P +P L +D+DTS+FD  ED   DV+T           +  F+G  + F GF+Y R      EN K +    + I  + +L+S  R
Sbjct:   14 RKLEALIREQRSALNLESLLDSVNALALDLNHPA-LRKNKNIDAFLSRYEKAVSRLRELQARLDDFDRIKLIGRGAYGAVQLVRHKVSKQVYAMKQLSKFEMIKRSDSAFFWEERDIMAFSNSPWIVQLCCAFQDERHLYLVMEFMPGGDLVTLTMNYD-MPEKWARFYTAEVVMALDAIHSLGFIHRDIKPDNMLLDRTGHLKLADFGTCMKMDK-TGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFLYELLVGDTPFYAESLVGTYGKIMDHKNSLTFPDDVEMSKEAKSLICAFLTDREVRLGRT-GVEEIKCHPFFKND--QWTFDTIRDTMAPVVPELNSDIDTSHFDDIEDEKGDVET--------FPPARAFVGNQLPFVGFTYFR------ENHKLEEKFKNEIQVKGELESKCR 427          

HSP 2 Score: 51.9878 bits (123), Expect = 5.665e-6
Identity = 28/68 (41.18%), Postives = 40/68 (58.82%), Query Frame = 3
Query: 2967 KKMSWEKRFVRLEEQNLCFYG--ED-VQTCHFRLL-LSSLIFVRSVSNVDVIHAKSSEIPRIFQICYD 3158
            K+  W+K++V +  + + FY   ED  Q+  F +L +  L  VR V+  DV  A + EIPRIFQI YD
Sbjct: 1061 KRFGWDKKYVVMSSKKILFYDSEEDREQSNPFMILDIDKLFHVRPVTQTDVYRADAREIPRIFQILYD 1128          
BLAST of Rho-associated protein kinase vs. Ensembl Cavefish
Match: rock2a (rho-associated protein kinase 2 [Source:NCBI gene;Acc:103042784])

HSP 1 Score: 363.999 bits (933), Expect = 7.572e-105
Identity = 166/345 (48.12%), Postives = 239/345 (69.28%), Query Frame = 3
Query:  234 DFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD-FGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            + +MK  DF+ +K+IG+GAFG+VQLVRH+ +  ++A+K+L+K +++    + F+ EERDIM   DS W+V+L  AFQD + LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+V+A++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V+C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV ++E+  G+ PFY+D+L+ TY KIM H+ SL+FP D E+S  A + +   + DR VRLG+     +IKSH FF N   +WT+DTI+D+ +P +P L +D+DTS FD   ED  D               F+G  + F GF+Y +
Sbjct:    6 ELQMKPEDFDRVKVIGRGAFGEVQLVRHRASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFADSPWVVQLCCAFQDDRYLYMVMEYMPGGDLVNLTSTYDVP-EKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRNGHLKLADFGTCMKMDS-TGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLNFPDDVEISKEAKNIICAFLTDREVRLGRN-GVGEIKSHPFFKND--QWTFDTIRDTAAPVVPELSSDIDTSNFDEIEEDKGDVETFPTPK------AFVGNQLPFVGFTYFK 339          

HSP 2 Score: 55.4546 bits (132), Expect = 4.789e-7
Identity = 28/76 (36.84%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCH----FRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L + V+N  K+  WE+++V +  + + FY  +    H      L +  L  VRSV+  DV  A + EIPRIFQI Y
Sbjct: 1079 LSLPVRNNTKRFGWERKYVVVSSKKILFYNSEQDKEHSNPYMVLDIDKLFHVRSVTQTDVYRADAKEIPRIFQILY 1154          
BLAST of Rho-associated protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004595.1 (pep scaffold:Pmarinus_7.0:GL477044:11103:63873:-1 gene:ENSPMAG00000004137.1 transcript:ENSPMAT00000004595.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 369.777 bits (948), Expect = 2.116e-107
Identity = 175/385 (45.45%), Postives = 257/385 (66.75%), Query Frame = 3
Query:  117 LFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDD--VDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            L D    L LD      L  NK   +F +++ +   K+ D RM+  D++ +K+IG+GAFG+VQLVRHK T  ++A+K+LNK +++    + F+ EERDIM   +S WIV+L Y+FQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ G++HRD+KPDN+L+D  GH+KLADFG+C+K++     V C+ A+GTPDYISPEVL SQ     YG+E DWW++GV L+E+  G+ PFY+++L+ TYGKIM H++SL+FP D ++S  A   +   + +R VRLG+   A +IK H FF N   +WTW+ I+++++P +P L +D DTS FD  E+D   D  F        +   F+G  + F GF+Y +
Sbjct:    1 LQDGLYALVLDL-DFPALRKNKNVESFLQRYGEAVGKVRDLRMRADDYDSVKVIGRGAFGEVQLVRHKVTKSVYAMKLLNKLEMIKRSDSAFFWEERDIMAFANSPWIVQLFYSFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLAVDAIHSMGYIHRDVKPDNMLLDKSGHLKLADFGTCMKLNK-DGMVRCDTAVGTPDYISPEVLRSQGGQGYYGRECDWWSVGVFLFEMLVGDTPFYAESLVGTYGKIMDHKKSLTFPVDLDISKNAKSLICAFLTERDVRLGKNGFA-EIKCHDFFKND--QWTWENIRETSAPVVPELSSDTDTSNFDEIENDKSADETF-------PQPKAFVGNQLPFVGFTYYK 372          
BLAST of Rho-associated protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003561.1 (pep scaffold:Pmarinus_7.0:GL478057:5187:43749:1 gene:ENSPMAG00000003167.1 transcript:ENSPMAT00000003561.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 363.229 bits (931), Expect = 4.804e-105
Identity = 177/401 (44.14%), Postives = 260/401 (64.84%), Query Frame = 3
Query:   63 NNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDV--DTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
               + +V D +  ++ + L  S +N  +          NK   NF  +++K   K+ D RM+ +D++++K+IG+GAFG+VQLVRHK T  ++A+K+L+K ++     + F+ EERDIM   +S W+V+L YAFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV LYE+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VR+G+     +IK H FF N   +WTW+ I+++ +P +P L +D DTS FD  E+D   D  F            F G  + F GF+Y
Sbjct:   12 GRLEGMVKDPRSEVNAEGLL-SRKNPLVAPSGFPAFFRNKNIDNFLNRYEKAVGKVRDLRMRANDYDVVKVIGRGAFGEVQLVRHKATKKVYAMKLLSKFEMTKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDHRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKNGHLKLADFGTCMKMD-TEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNALNFPDDVDISKDAKNLICAFLIDREVRVGRN-GVGEIKRHPFFKND--QWTWENIRETAAPVVPELVSDTDTSNFDEIEEDKAEDETFPV-------PKAFSGNQLPFVGFTY 399          

HSP 2 Score: 51.6026 bits (122), Expect = 1.549e-6
Identity = 27/76 (35.53%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGED----VQTCHFRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L V  KN  K+  W+K++V +  + + FY ++    + T +  L +  L  VR V+  DV  A++ +IPRIFQI Y
Sbjct: 1148 LSVPNKNNIKRYGWKKQYVVVSSKKILFYNDEQDKELSTPYMVLDIDKLFHVRPVTQGDVYRAEAKDIPRIFQILY 1223          
BLAST of Rho-associated protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006413.1 (pep scaffold:Pmarinus_7.0:GL485553:3974:11158:-1 gene:ENSPMAG00000005799.1 transcript:ENSPMAT00000006413.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 310.071 bits (793), Expect = 9.943e-96
Identity = 164/350 (46.86%), Postives = 220/350 (62.86%), Query Frame = 3
Query:  210 KKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSY 1259
            +K   K+ D RM   DF LLK+IG+GAFG VQLVRHKQT  + A+K LNK +++    + F+ EERDIM   +S WI++L  AFQD + LYMVM+Y+PGG+LV+ MS  +V  EK   FY AE+VLA++ +H+ GF+HRDIKPDN+L+D  GH+KLADFG+C+KMD     V  N A+GTPDYISPEVL SQ  +  YG   +   +G  L     G+ PFY+++L+ TY KIM H  SL FP D  VS  A   +   + DR VRLG+T    +IK H FF N   +WTW  I+++ +P +P L +D D S F    DD+      +         F+G  + F GF+Y
Sbjct:    1 EKAVGKVHDLRMNVHDFELLKVIGRGAFGVVQLVRHKQTRRVHAMKRLNKGEMLKRSDSAFFWEERDIMAFANSSWIIQLFSAFQDERYLYMVMEYMPGGDLVSLMSNYDV-PEKWARFYTAEVVLALDAIHSMGFIHRDIKPDNMLLDRLGHLKLADFGTCMKMD-AEGMVRSNTAVGTPDYISPEVLKSQGGDGYYGLH-EPPEIGNHLVAAHAGDTPFYAESLVGTYSKIMDHRNSLCFPDDASVSKEARSLICAFLTDREVRLGRT-GVDEIKRHPFFRND--QWTWQNIRETVAPVVPELASDADASCF----DDISEQEKAEEEAFSVPKAFLGNQLPFIGFTY 340          
BLAST of Rho-associated protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002084.1 (pep scaffold:Pmarinus_7.0:GL476486:6827:40506:-1 gene:ENSPMAG00000001890.1 transcript:ENSPMAT00000002084.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 315.849 bits (808), Expect = 7.769e-94
Identity = 156/350 (44.57%), Postives = 228/350 (65.14%), Query Frame = 3
Query:  225 KLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVC-FYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNT-KYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDD-EVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQ--GLKSTTFIGENIQFSGFSY 1259
            ++ + R+   DF +LK+IG+GAFG+V +V+ K +  +FA+KILNK +++    T  ++EERD++++ DS+WI  LHYAFQD   LY+VMDY  GG+L+  +SK E  L +++  FY AE+VLAI+ +H   +VHRDIKPDN+L+D+ GHI+LADFGSC+K+      V  + A+GTPDYISPE+L +  D   KYG E DWW+LGV +YE+ +GE PFY+++L+ TYGKIM H++   FP +  EVS    D ++ LIC R  R G+     D K+H FF        WD I++  +P+IP + +  DTS F     DVD ++L  +        T F G ++ F GF++
Sbjct:    7 RVKEMRLHREDFEMLKVIGRGAFGEVAVVKLKSSEKVFAMKILNKWEMLKRAETACFREERDVLVRGDSQWITTLHYAFQDENYLYLVMDYYVGGDLLTLLSKFEDRLPEEMAKFYVAEMVLAIHSIHELKYVHRDIKPDNVLLDMNGHIRLADFGSCLKL-LTDGTVQSSVAVGTPDYISPEILQAMEDGKGKYGPECDWWSLGVCMYEMLFGETPFYAESLVETYGKIMNHKERFQFPTNSVEVSDDGKDLIRHLICSREQRFGRN-GIQDFKNHPFFAG----IDWDNIRNCEAPYIPEVSSPTDTSNF-----DVDDDYLKNTEAVPPPSHTAFSGLHLPFVGFTF 345          
BLAST of Rho-associated protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005559.1 (pep scaffold:Pmarinus_7.0:GL476474:6865:57025:1 gene:ENSPMAG00000004999.1 transcript:ENSPMAT00000005559.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 282.337 bits (721), Expect = 1.601e-77
Identity = 164/446 (36.77%), Postives = 245/446 (54.93%), Query Frame = 3
Query:   42 NNYVLLENNFQNVVSDDKENISL---DLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLL-EKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIIN---EDKLQSTNRE 1358
            + ++ L   FQ  ++   ++  L   D L DS   LF +C S   L  + +  NF  +H +   +L + R+  SDF +  ++ +G   +VQ+VR + TGD++A+K+L KS ++  +    ++EERD++ +  S W+  L YAFQD   LY+VM+Y PGG+L+  + + E  L E  V FY AE+VLA   LH  G+VHRD+KP+N+L+D  GHIKLADFGS  K++   K V+     G+PDYI+PEVL S S    YG E DWW++GV+ YE+ YG+ PF       T   I   ++ + FP +  VS   +D +K L+C R  RL   K    +  H FF        W+ IQ    PF+P L+ D DTS F+  E + +      + + L+   F G N+ F GFSY +        +   +VL+S   N   E KLQ+  +E
Sbjct:   22 SRFLRLNQLFQGKIAQPGQHRYLATRDGLLDSLLVLFEEC-SDPGLMKHNHIANFVSKHAETVSELQELRVTSSDFEVRGVVSRGHHSEVQVVRERATGDVYAMKVLQKSLLLHRDNVALFEEERDVLARASSPWLTHLQYAFQDEDNLYLVMEYHPGGDLLGLLGRYEDQLDESMVRFYLAELVLATQSLHQMGYVHRDLKPENVLVDRTGHIKLADFGSAAKLN-ANKMVSSKLPEGSPDYIAPEVLSSFSGKGSYGPECDWWSVGVIAYEMVYGKTPFAEGISTKTISNIKNFQKFVRFPSEPAVSVEFVDLVKGLLCGRVDRLTPDK----LLCHPFFAG----IDWNNIQHMPPPFVPPLQGDDDTSNFE--EVERERPAAPPTPRQLRQPGFSGNNLPFIGFSYSKALVGLSRTESVMSVLDSPAKNSSIEKKLQTKAKE 455          
BLAST of Rho-associated protein kinase vs. Ensembl Yeast
Match: TPK3 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication [Source:SGD;Acc:S000001649])

HSP 1 Score: 203.371 bits (516), Expect = 9.807e-58
Identity = 119/306 (38.89%), Postives = 176/306 (57.52%), Query Frame = 3
Query:  252 SDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLIC-DRSVRLGQTKDAS-DIKSHIFFNNSSREWTWDTIQDS--TSPF-IPVLENDLDTSYFD 1154
            SDF +L+ +G G+FG+V L+R    G  +ALK L K  +V  +      +ER ++  +   +I+++   FQD ++++MVMDY+ GG L + + K +        FYAAE+ LA+  LH+   ++RD+KP+NIL+D  GHIK+ DFG   K  P   Y  C    GTPDYI+PEV+ ++     Y K VDWW+ GV++YE+  G  PFY+ N + TY  I+  E  L FPP       A D +KKLI  D S RLG  ++ S D+K+H +FN    E  W+ +      +P+  P+ +   DTS FD
Sbjct:   86 SDFQILRTLGTGSFGRVHLIRSNHNGRFYALKTLKKHTIVKLKQVEHTNDERRMLSIVSHPFIIRMWGTFQDSQQVFMVMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFG-FAKYVPDVTYTLC----GTPDYIAPEVVSTKP----YNKSVDWWSFGVLIYEMLAGYTPFYNSNTMKTYENILNAE--LKFPPFFHPD--AQDLLKKLITRDLSERLGNLQNGSEDVKNHPWFN----EVIWEKLLARYIETPYEPPIQQGQGDTSQFD 374          
BLAST of Rho-associated protein kinase vs. Ensembl Yeast
Match: TPK1 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication [Source:SGD;Acc:S000003700])

HSP 1 Score: 202.986 bits (515), Expect = 1.570e-57
Identity = 117/320 (36.56%), Postives = 190/320 (59.38%), Query Frame = 3
Query:  246 KYS--DFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPP--DDEVSGMALDFMKKLIC-DRSVRLGQTKDAS-DIKSHIFFNNSSREWTWDTI--QDSTSPF-IPVLENDLDTSYFD-FGEDDVD 1175
            KYS  DF +L+ +G G+FG+V L+R +  G  +A+K+L K  VV  +      +ER ++  +   +I+++   FQD ++++M+MDY+ GG L + + K +        FYAAE+ LA+  LH+   ++RD+KP+NIL+D  GHIK+ DFG   K  P   Y  C    GTPDYI+PEV+ ++     Y K +DWW+ G+++YE+  G  PFY  N + TY KI+  E  L FPP  +++V     D + +LI  D S RLG  ++ + D+K+H +F    +E  W+ +  ++  +P+  P+ +   DTS FD + E+D++
Sbjct:   81 KYSLQDFQILRTLGTGSFGRVHLIRSRHNGRYYAMKVLKKEIVVRLKQVEHTNDERLMLSIVTHPFIIRMWGTFQDAQQIFMIMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFG-FAKYVPDVTYTLC----GTPDYIAPEVVSTKP----YNKSIDWWSFGILIYEMLAGYTPFYDSNTMKTYEKILNAE--LRFPPFFNEDVK----DLLSRLITRDLSQRLGNLQNGTEDVKNHPWF----KEVVWEKLLSRNIETPYEPPIQQGQGDTSQFDKYPEEDIN 381          
BLAST of Rho-associated protein kinase vs. Ensembl Yeast
Match: YPK2 (Protein kinase similar to S/T protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); human homolog SGK2 can complement a ypk1 ypk2 double mutant [Source:SGD;Acc:S000004710])

HSP 1 Score: 208.379 bits (529), Expect = 6.617e-57
Identity = 144/438 (32.88%), Postives = 235/438 (53.65%), Query Frame = 3
Query:   24 DKMDKLNNYVLLENNFQNVVSDDKE---------NISLDLLFDSFQ---NLFLDCKSVSNLPTNKYFTNFYKQHKKV-----YEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVT-CEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLIC-DRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD--FGE----DDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYI 1262
            D   ++ + +L   N+Q  + +  E         N + D+  DSF    NL +D  +   L  N ++ +  + + K+     Y+   +  +   DF+LLK+IG+G+FG+V  VR K T  ++ALK L K+ +V+ CE T     ER ++ ++D  +IV L ++FQ P++LY+V+ ++ GG L   +            FY AE++ A++ LH    ++RD+KP+NIL+D +GHI L DFG C K++ +      +   GTP+Y++PE+LL Q     Y K VDWW LG++LYE+  G PP+Y +N+   Y KI+  +Q L FP  D     A D +  L+  D S RLG      +I++H FF + S  W    ++    P+ P++++++DT+ FD  F +    D V   +L+ S Q            QF G++YI
Sbjct:  250 DVFARIPSLLLPSKNWQQEIGEQDEVLKEILKKINTNQDIHLDSFHLPLNLKIDSAAQIRL-YNHHWISLERGYGKLNITVDYKPSKNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKIYALKALRKAYIVSKCEVT-HTLAERTVLARVDCPFIVPLKFSFQSPEKLYLVLAFINGGELFYHLQHEGRFSLARSRFYIAELLCALDSLHKLDVIYRDLKPENILLDYQGHIALCDFGLC-KLN-MKDNDKTDTFCGTPEYLAPEILLGQG----YTKTVDWWTLGILLYEMMTGLPPYYDENVPVMYKKIL--QQPLLFP--DGFDPAAKDLLIGLLSRDPSRRLG-VNGTDEIRNHPFFKDIS--WKKLLLKGYIPPYKPIVKSEIDTANFDQEFTKEKPIDSVVDEYLSASIQK-----------QFGGWTYI 661          
BLAST of Rho-associated protein kinase vs. Ensembl Yeast
Match: TPK2 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000006124])

HSP 1 Score: 200.29 bits (508), Expect = 9.043e-57
Identity = 114/313 (36.42%), Postives = 185/313 (59.11%), Query Frame = 3
Query:  255 DFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLI-CDRSVRLGQTKDAS-DIKSHIFFNNSSREWTWDTI--QDSTSPFIPVLENDL-DTSYFD-FGEDDVD 1175
            DF +++ +G G+FG+V LVR    G  +A+K+L K QVV  +      +ER ++  ++  +++++   FQD + ++MVMDY+ GG L + + K +        FYAAE++LA+  LH    ++RD+KP+NIL+D  GHIK+ DFG   ++  VT +  C    GTPDYI+PEV+ ++     Y K VDWW+LGV++YE+  G  PFY    + TY KI+  +  + +PP        +D + KLI  D + R+G  +  S DIK+H +F+    E  W+ +  +D  +P+ P + + + DTS FD + E+ +D
Sbjct:   69 DFQIMRTLGTGSFGRVHLVRSVHNGRYYAIKVLKKQQVVKMKQVEHTNDERRMLKLVEHPFLIRMWGTFQDARNIFMVMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVILALEYLHAHNIIYRDLKPENILLDRNGHIKITDFGFAKEVQTVT-WTLC----GTPDYIAPEVITTKP----YNKSVDWWSLGVLIYEMLAGYTPFYDTTPMKTYEKIL--QGKVVYPP--YFHPDVVDLLSKLITADLTRRIGNLQSGSRDIKAHPWFS----EVVWERLLAKDIETPYEPPITSGIGDTSLFDQYPEEQLD 364          
BLAST of Rho-associated protein kinase vs. Ensembl Yeast
Match: YPK1 (S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant [Source:SGD;Acc:S000001609])

HSP 1 Score: 204.527 bits (519), Expect = 1.681e-55
Identity = 127/346 (36.71%), Postives = 192/346 (55.49%), Query Frame = 3
Query:  255 DFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSC---VKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLIC-DRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD--FGE----DDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYI 1262
            DF+LLK+IG+G+FG+V  VR K T  ++ALK + KS +V+         ER ++ ++D  +IV L ++FQ P++LY V+ ++ GG L   + K          FY AE++ A++ LH    V+RD+KP+NIL+D +GHI L DFG C   +K D  T     +   GTP+Y++PE+LL       Y K VDWW LGV+LYE+  G PP+Y +++   Y KI+  ++ L FP  D     A D +  L+  D + RLG    A +I++H FF+  S  W    ++    P+ P + N +DTS FD  F      D V   +L++S Q            QF G++Y+
Sbjct:  346 DFDLLKVIGKGSFGKVMQVRKKDTQKVYALKAIRKSYIVSKSEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYFVLAFINGGELFYHLQKEGRFDLSRARFYTAELLCALDNLHKLDVVYRDLKPENILLDYQGHIALCDFGLCKLNMKDDDKT-----DTFCGTPEYLAPELLLGLG----YTKAVDWWTLGVLLYEMLTGLPPYYDEDVPKMYKKIL--QEPLVFP--DGFDRDAKDLLIGLLSRDPTRRLGYN-GADEIRNHPFFSQLS--WKRLLMKGYIPPYKPAVSNSMDTSNFDEEFTREKPIDSVVDEYLSESVQK-----------QFGGWTYV 664          
BLAST of Rho-associated protein kinase vs. Ensembl Nematostella
Match: EDO37677 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF23])

HSP 1 Score: 296.204 bits (757), Expect = 1.640e-89
Identity = 161/391 (41.18%), Postives = 241/391 (61.64%), Query Frame = 3
Query:  102 ISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVC-FYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQ-SDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVD-TNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            +S + L D+F  LF +C S      +K    F K+++ +  +L    +  +DFN++  IG+G FG+VQ+V+ K TGD++A+K L KSQ ++ E   F++EER+IM   ++ WI  L YAFQD   LY+VMDY PGG+L++ +SK + + E+D+  FY AEIV+AI+ LH  GFVHRD+KPDN+LID  GHIKLADFGS  ++    K V     +GTP+YI+PEVL S       YG E DWW+LGV+ YE+ +G+ PF +D+++ TY KIM  + SL FP + ++S  A + +  L+     R+G       +  H FFN       W+ +Q++  P++P L+   DTS FD  E D D  + L + +   +   F G+++ F GFS+I+
Sbjct:   30 VSREALLDAFTVLFEECSSDHMKKKDKNIATFVKKYQPMINELRRLSLSKNDFNVVNTIGRGHFGEVQVVKDKATGDVYAMKTLKKSQTLSEEAVAFFEEEREIMATANNPWITSLQYAFQDSHSLYLVMDYHPGGDLLSLLSKYDDIFEEDMARFYLAEIVMAIHSLHTMGFVHRDVKPDNVLIDRTGHIKLADFGSSARLS-ADKKVFSKMPVGTPEYIAPEVLTSMDGSGGAYGVECDWWSLGVVAYEMLFGQTPFEADSVVVTYSKIMNFKSSLRFPSEPKISEDAKELIHNLLTSSEGRIGY----EGLGCHKFFNGIE----WNKLQNTLPPYVPTLDGTDDTSNFDEFEPDSDEMDQLKKYSFCSEEKGFKGKDLPFVGFSFIK 411          
BLAST of Rho-associated protein kinase vs. Ensembl Nematostella
Match: EDO32008 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW88])

HSP 1 Score: 282.337 bits (721), Expect = 1.760e-86
Identity = 137/277 (49.46%), Postives = 191/277 (68.95%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTY 899
             +N+V D +  + +D L D  + L  DC +   L  NK   NF +++++    +   R+   DF  LK+IG+GAFG+VQLVRH  T  ++A+K+L+K +++    + F+ EER+IM   +S WIVKLHYAFQD K LYMVMDY+ GG+LVN MS  E+  EK   FY AE+VLA++ +H  GFVHRD+KPDN+L+D +GH+KLADFG+C++MD     V  + A+GTPDYISPEVL SQ     YG+E DWW++GV LYE+  G+ PFY+D+L+ TY
Sbjct:    5 LENIVRDSRSPLYVDGLLDGIEALVNDC-NFPQLRRNKNVENFLQRYEQPTRVIQTHRLNNDDFKTLKVIGRGAFGEVQLVRHTHTKKVYAMKLLSKFEMIKRSDSAFFWEEREIMAHSNSNWIVKLHYAFQDEKYLYMVMDYMSGGDLVNLMSNYEI-PEKWAKFYCAEVVLALDAIHTMGFVHRDVKPDNMLLDGEGHLKLADFGTCMRMDR-DGMVRSDTAVGTPDYISPEVLKSQGGEGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTY 278          
BLAST of Rho-associated protein kinase vs. Ensembl Nematostella
Match: EDO42700 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0M7])

HSP 1 Score: 239.195 bits (609), Expect = 6.756e-69
Identity = 130/365 (35.62%), Postives = 207/365 (56.71%), Query Frame = 3
Query:  192 NFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKM-----------------------DPVTKYVNC-----NR------AIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD-FGEDDVDTN 1181
              + Q +  + +L   R+   DF+ LK+IG+GAFG+V+LV+ + TG ++A+KIL K+ ++  E     + ERDI+ + +++W+VK++Y+FQD   L++VM++LPGG+L+  + K +   E++  FY AE +LAI+ +H  GF+HRDIKPDN+L+D +GHIKLADFG C  +                       DP+           NR       +GTPDYI+PEV + Q     Y K  D+W+LGV++YE+  G PPF S++   TY K+M   ++L FPP+  +S  A D + +          +  +  DI++  FF        W+ ++D  +     +++  DTS FD F E D+DT+
Sbjct:   72 KLHAQKETEFLRLKRSRIGKEDFDSLKVIGRGAFGEVRLVQKQDTGHVYAMKILRKADMLEKEQVAHARAERDILAEAENQWVVKMYYSFQDDYYLFLVMEFLPGGDLMTLLMKKDTFTEEETRFYIAEALLAIDSIHQLGFIHRDIKPDNLLLDSRGHIKLADFGLCTGLKKAHRTEFYRDISPADIKDFSNGDPLDSKRRAATWKRNRRQLAYSTVGTPDYIAPEVFIQQG----YTKSCDFWSLGVIMYEMLIGYPPFCSESPQETYKKVMNWRETLVFPPEMPISPNARDLISR---------QRLSNIEDIRAQPFFKYVD----WENLRDRPAALPIQVKSIDDTSNFDEFPETDLDTS 419          
BLAST of Rho-associated protein kinase vs. Ensembl Nematostella
Match: EDO36034 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJR1])

HSP 1 Score: 235.343 bits (599), Expect = 5.286e-68
Identity = 144/390 (36.92%), Postives = 203/390 (52.05%), Query Frame = 3
Query:  201 KQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCV------------------------------------------------KMDPV-----TKYVNC--NRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQG 1205
            KQ +  Y +L   +M  S F  ++ IG GAFG+V LVR   T  L+A+KIL KS+V         + ERDI+ + D+ W+VKL+Y+FQD + LY VMDY+PGG+L++ + K  +  E    FY AE+VLAI+ +H  GFVHRDIKPDNILID  GHIKL DFG C                                                 +M P+      K++ C  +  +GTP+YI+PEVLL       Y +  DWW++GV+LYE+  G+PPF +     T  KI+  E  L  P    +S  A D + +L  +  +RLG +  AS+IK+H FF  +  +W +   +   +P+ PV+    DT+ F    D VD   L  S+ G
Sbjct:   19 KQKETNYIRLKRTKMDISMFEKIRTIGIGAFGEVWLVRKTDTSMLYAMKILRKSEVFRRNQAAHVKAERDILAEADNEWVVKLYYSFQDQENLYFVMDYVPGGDLMSLLIKFGIFPEDYAKFYIAELVLAIDSVHRMGFVHRDIKPDNILIDKDGHIKLTDFGLCTGFRWTHDSKYYEKGQHMRQDSMEPDERAWEEMRATCHCGDISIDKPYSEMKPLERRHFRKHMRCQAHSLVGTPNYIAPEVLL----RIGYRQSCDWWSVGVILYEMLVGQPPFLAPTPAETQLKIINWETELRIPHQARLSREAKDLILRLCTNADMRLG-SNGASEIKAHPFF--ACVDWAY-LAKRVQAPYTPVIRFPTDTANF----DPVDPERLRTSDSG 396          
BLAST of Rho-associated protein kinase vs. Ensembl Nematostella
Match: EDO37800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEW2])

HSP 1 Score: 204.527 bits (519), Expect = 1.612e-57
Identity = 123/346 (35.55%), Postives = 185/346 (53.47%), Query Frame = 3
Query:  240 RMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMK-IDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLIC-DRSVRLGQTK-DASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFD--FGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYI 1262
            + K SDF  LK+IG+G+FG+V L ++KQ    +A+K+LNKS +           ER+++++ +   ++V LHY+FQ   +LY V+DY+ GG L   + +     E    FYAAEI  AI  LH    ++RD+KP+NIL+D KGHI L DFG C   + + +    +   GTP+Y++PEVL  Q     Y K VDWW LG +LYE+ YG PPFYS +    Y  I+     L       +S  A   ++ L+  D++ RLG  + D  DI++H FF   S  W     +    P+ P +   LD  +FD  F  + V  +    ++    S +    +  F GF+Y+
Sbjct:   68 KAKPSDFLFLKVIGKGSFGKVFLSKNKQEDKFYAIKVLNKSAIRKRNEAKHIMAERNVLVQNVTHPFLVGLHYSFQTKDKLYFVLDYVNGGELFFHLQRERYFPESRARFYAAEIASAIGYLHGLQIIYRDLKPENILLDSKGHIVLTDFGLC--KEGIDRSGTTSTFCGTPEYLAPEVLRKQD----YDKSVDWWCLGAVLYEMMYGLPPFYSRDTAEMYDNILHKPLRLR----TNISQSARHILEGLLQKDKTKRLGSGEGDFEDIRTHSFFR--SINWQQLVARGIEPPYNPNVSGQLDLKHFDPEFVREPVPGSVGKSADSNFVSASVDDPDDAFVGFTYV 401          
BLAST of Rho-associated protein kinase vs. Ensembl Medaka
Match: rock1 (Rho associated coiled-coil containing protein kinase 1 [Source:NCBI gene;Acc:101158388])

HSP 1 Score: 389.423 bits (999), Expect = 1.488e-113
Identity = 184/415 (44.34%), Postives = 270/415 (65.06%), Query Frame = 3
Query:   39 LNNYVLLENNFQNV---VSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKST-----TFIGENIQFSGFSY 1259
            +++ + +E  F+ +   + D K  I+ D L D    L  D      L  NK   NF  ++K+   K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L +AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +WTW+ I+D+ +P +P L +D DTS FD  E+D          +G + T      F+G  + F GF+Y
Sbjct:    1 MSSEINMEGRFEKIDAMLKDPKSEINTDCLLDGLDALVYDL-DFPALRKNKSIDNFLNRYKETISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSSWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNK-DGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRN-GVDEIKRHPFFKND--QWTWENIRDTAAPVVPELSSDTDTSNFDDIEED----------RGEEETFPIPKAFVGNQLPFVGFTY 399          
BLAST of Rho-associated protein kinase vs. Ensembl Medaka
Match: rock1 (Rho associated coiled-coil containing protein kinase 1 [Source:NCBI gene;Acc:101158388])

HSP 1 Score: 382.104 bits (980), Expect = 3.717e-111
Identity = 176/380 (46.32%), Postives = 257/380 (67.63%), Query Frame = 3
Query:   39 LNNYVLLENNFQNV---VSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDD 1169
            +++ + +E  F+ +   + D K  I+ D L D    L  D      L  NK   NF  ++K+   K+ D RMK  D+ ++K+IG+GAFG+VQLVRHK T  ++A+K+L+K +++    + F+ EERDIM   +S W+V+L +AFQD + LYMVM+Y+PGG+LVN MS  +V  EK   FY AE+VLA++G+H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KM+     V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV L+E+  G+ PFY+D+L+ TY KIM H+ +L+FP D ++S  A + +   + DR VRLG+     +IK H FF N   +WTW+ I+D+ +P +P L +D DTS FD  E+D
Sbjct:    1 MSSEINMEGRFEKIDAMLKDPKSEINTDCLLDGLDALVYDL-DFPALRKNKSIDNFLNRYKETISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKATRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSSWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDV-PEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNK-DGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRN-GVDEIKRHPFFKND--QWTWENIRDTAAPVVPELSSDTDTSNFDDIEED 374          
BLAST of Rho-associated protein kinase vs. Ensembl Medaka
Match: rock2a (rho-associated protein kinase 2 [Source:NCBI gene;Acc:101172697])

HSP 1 Score: 375.941 bits (964), Expect = 8.548e-109
Identity = 180/402 (44.78%), Postives = 265/402 (65.92%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             ++++ D +  ++L+ L DS   L LD      L  NK    F  +++KV E+L D +MK  DF  +K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EER IM   +S W+V+L  AFQD   LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+V+A++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV ++E+  G+ PFY+D+L+ TY KIM H+ SL FP + E+S  A + +   + DR VRLG+     +IK H FF N   +WT+D I+++ +P +P L +D+DTS FD  ED   DV+T    ++        F+G  + F GF+Y +
Sbjct:   22 LEDMLRDPRSALNLESLLDSLNALVLDL-DYPALRKNKNIETFLNRYEKVVEELRDLQMKSEDFEKVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERGIMAFANSPWVVQLCCAFQDEHYLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRLGHLKLADFGTCMKMD-ATGMVRCDTAVGTPDYISPEVLKSQGGDGCYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPEEVEISADAKNIICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWTFDNIRETVAPVVPELSSDIDTSNFDEIEDDKGDVETVPTPKA--------FVGNQLPFVGFTYFK 409          

HSP 2 Score: 53.9138 bits (128), Expect = 1.320e-6
Identity = 28/76 (36.84%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDV---QTCHFRLL-LSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L + V+N  KK  WE+++V +  + + FY  +    Q+  + +L +  L  VR V+  DV  A + EIPRIFQI Y
Sbjct: 1150 LSLPVRNNTKKFGWERKYVVVSSKKILFYNSEQDREQSIPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILY 1225          
BLAST of Rho-associated protein kinase vs. Ensembl Medaka
Match: rock2a (rho-associated protein kinase 2 [Source:NCBI gene;Acc:101172697])

HSP 1 Score: 375.941 bits (964), Expect = 1.340e-108
Identity = 180/402 (44.78%), Postives = 265/402 (65.92%), Query Frame = 3
Query:   69 FQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
             ++++ D +  ++L+ L DS   L LD      L  NK    F  +++KV E+L D +MK  DF  +K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EER IM   +S W+V+L  AFQD   LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+V+A++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV ++E+  G+ PFY+D+L+ TY KIM H+ SL FP + E+S  A + +   + DR VRLG+     +IK H FF N   +WT+D I+++ +P +P L +D+DTS FD  ED   DV+T    ++        F+G  + F GF+Y +
Sbjct:   22 LEDMLRDPRSALNLESLLDSLNALVLDL-DYPALRKNKNIETFLNRYEKVVEELRDLQMKSEDFEKVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERGIMAFANSPWVVQLCCAFQDEHYLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRLGHLKLADFGTCMKMD-ATGMVRCDTAVGTPDYISPEVLKSQGGDGCYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPEEVEISADAKNIICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWTFDNIRETVAPVVPELSSDIDTSNFDEIEDDKGDVETVPTPKA--------FVGNQLPFVGFTYFK 409          

HSP 2 Score: 53.9138 bits (128), Expect = 1.477e-6
Identity = 28/76 (36.84%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDV---QTCHFRLL-LSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L + V+N  KK  WE+++V +  + + FY  +    Q+  + +L +  L  VR V+  DV  A + EIPRIFQI Y
Sbjct: 1150 LSLPVRNNTKKFGWERKYVVVSSKKILFYNSEQDREQSIPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILY 1225          
BLAST of Rho-associated protein kinase vs. Ensembl Medaka
Match: rock2a (rho-associated protein kinase 2 [Source:NCBI gene;Acc:101172697])

HSP 1 Score: 369.777 bits (948), Expect = 8.930e-107
Identity = 178/386 (46.11%), Postives = 255/386 (66.06%), Query Frame = 3
Query:  117 LFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGED---DVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIR 1265
            L DS   L LD      L  NK    F  +++KV E+L D +MK  DF  +K+IG+GAFG+VQLVRHK +  ++A+K+L+K +++    + F+ EER IM   +S W+V+L  AFQD   LYMVM+Y+PGG+LVN  S  +V  EK   FY AE+V+A++ +H+ GF+HRD+KPDN+L+D  GH+KLADFG+C+KMD  T  V C+ A+GTPDYISPEVL SQ  +  YG+E DWW++GV ++E+  G+ PFY+D+L+ TY KIM H+ SL FP + E+S  A + +   + DR VRLG+     +IK H FF N   +WT+D I+++ +P +P L +D+DTS FD  ED   DV+T    ++        F+G  + F GF+Y +
Sbjct:    9 LMDSLNALVLDL-DYPALRKNKNIETFLNRYEKVVEELRDLQMKSEDFEKVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERGIMAFANSPWVVQLCCAFQDEHYLYMVMEYMPGGDLVNLTSTYDV-PEKWAKFYTAEVVMALDAIHSMGFIHRDVKPDNMLLDRLGHLKLADFGTCMKMD-ATGMVRCDTAVGTPDYISPEVLKSQGGDGCYGRECDWWSVGVFIFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPEEVEISADAKNIICAFLTDREVRLGRN-GVEEIKQHPFFKND--QWTFDNIRETVAPVVPELSSDIDTSNFDEIEDDKGDVETVPTPKA--------FVGNQLPFVGFTYFK 380          

HSP 2 Score: 53.9138 bits (128), Expect = 1.419e-6
Identity = 28/76 (36.84%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 2940 LEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDV---QTCHFRLL-LSSLIFVRSVSNVDVIHAKSSEIPRIFQICY 3155
            L + V+N  KK  WE+++V +  + + FY  +    Q+  + +L +  L  VR V+  DV  A + EIPRIFQI Y
Sbjct: 1121 LSLPVRNNTKKFGWERKYVVVSSKKILFYNSEQDREQSIPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILY 1196          
BLAST of Rho-associated protein kinase vs. Planmine SMEST
Match: SMESG000041847.1 (SMESG000041847.1)

HSP 1 Score: 2408.64 bits (6241), Expect = 0.000e+0
Identity = 1264/1268 (99.68%), Postives = 1268/1268 (100.00%), Query Frame = 3
Query:   30 MDKLNNYVLLENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTNRENEXXXXXXXXXXXXXXXXENQLHVVSTNFRKEEIISKKLQIDFSDLLTQHSCCQKTIEELETIREKLNSDLVIXXXXXXXXXXXXXXXXXXVNGYLKATNLKLMEVQTKFHESKLRCVQLESDYNNLIESHNLLEKDLVRLRNFISSKKDEESSINDSDFIQLKSEYESKDYDLQKSLLENKCIAKSLESLQNEKEKLSYSLIELEAKFTEQLHENRRQETELKEKEDLIKEMKDELSRLKVSIDESLKKEQNTHVKYSNLMKLHTQEKNKFEQELEEQRNKLSKIITDKTSEIKLINSSVETMKNSNTKLLKDLEMAKVKQESLVEKIKDIIHGQSTTAKEMMISEDLKSGFLGKRSGRIYHQLAKENKRLENLLDEFKKTSQCKEKELMKQVETITREKCQLELELSSSQDGYYENLNSHDISCNRAXXXXXXXXXXXFDSGFPHDSSLVSQSNFRRSIKNPFSKPPLLSHKASHLKQDLTNDVFPESGDENNGVKLRQKKSTNLQNRNSKTESMTMDFRNINQILEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDVQTCHFRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICYDKNDSLYLTAQMNPISKSTTSINSAESLXXXXXXXXXXXXXXXXXXXDFRKTSLARFLPIKGFKXXXXXXXXXXXXXXXXXFPVFLKGHIFTIITTETRSSCLICNKTFNINSGVGLSSSSTMTNAQKSLTNSFSIDSQTPMTCVTQHPDKIYQCRHCLLRIHGYDLDSILFNCTKSVVIQLCRAPSEIAQVNWVKYLNQFIKTQSKSICDSGMSTPNTKPRSSKN 3833
            MDKLNNYVLLENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTN+ENEISKDILDKLMIDKKDLENQLHVVSTNFRKEEIISKKLQIDFSDLLTQHSCCQKTIEELETIREKLNSDLVIQQSSSEQEKELLTKELTEVNGYLKATNLKLMEVQTKFHESKLRCVQLESDYNNLIESHNLLEKDLVRLRNFISSKKDEESSINDSDFIQLKSEYESKDYDLQKSLLENKCIAKSLESLQNEKEKLSYSLIELEAKFTEQLHENRRQETELKEKEDLIKEMKDELSRLKVSIDESLKKEQNTHVKYSNLMKLHTQEKNKFEQELEEQRNKLSKIITDKTSEIKLINSSVETMK+SNTKLLKDLEMAKVKQESLVEKIKDIIHGQSTTAKEMMISEDLKSGFLGKRSGRIYHQLAKENKRLENLLDEFKKTSQCKEKELMKQVETITREKCQLELELSSSQDGYYENLNSHDISCNRASVSTIASSHSSFDSGFPHDSSLVSQSNFRRSIKNPFSKPPLLSHKASHLKQDLTNDVFPESGDENNGVKLRQKKSTNLQNRNSKTESMTMDFRNINQILEVNVKNKGKKMSWEKRFVRLEEQNLCFYGED+QTCHFRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICYDKNDSLYLTAQMNPISKSTTSINSAESLKSSNSSNENEVNVTVTEKKDFRKTSLARFLPIKGFKSNKNKENPELPNNLSNNFPVFLKGHIFTIITTETRSSC+ICNKTFNINSGVGLSSSSTMTNAQKSLTNSFSIDSQTPMTCVTQHPDKIYQCRHCLLRIHGYDLDSILFNCTKSVVIQLCRAPSEIAQVNWVKYLNQFIKTQSKSICDSGMSTPNTKPRSSKN
Sbjct:    1 MDKLNNYVLLENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTNKENEISKDILDKLMIDKKDLENQLHVVSTNFRKEEIISKKLQIDFSDLLTQHSCCQKTIEELETIREKLNSDLVIQQSSSEQEKELLTKELTEVNGYLKATNLKLMEVQTKFHESKLRCVQLESDYNNLIESHNLLEKDLVRLRNFISSKKDEESSINDSDFIQLKSEYESKDYDLQKSLLENKCIAKSLESLQNEKEKLSYSLIELEAKFTEQLHENRRQETELKEKEDLIKEMKDELSRLKVSIDESLKKEQNTHVKYSNLMKLHTQEKNKFEQELEEQRNKLSKIITDKTSEIKLINSSVETMKDSNTKLLKDLEMAKVKQESLVEKIKDIIHGQSTTAKEMMISEDLKSGFLGKRSGRIYHQLAKENKRLENLLDEFKKTSQCKEKELMKQVETITREKCQLELELSSSQDGYYENLNSHDISCNRASVSTIASSHSSFDSGFPHDSSLVSQSNFRRSIKNPFSKPPLLSHKASHLKQDLTNDVFPESGDENNGVKLRQKKSTNLQNRNSKTESMTMDFRNINQILEVNVKNKGKKMSWEKRFVRLEEQNLCFYGEDLQTCHFRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICYDKNDSLYLTAQMNPISKSTTSINSAESLKSSNSSNENEVNVTVTEKKDFRKTSLARFLPIKGFKSNKNKENPELPNNLSNNFPVFLKGHIFTIITTETRSSCVICNKTFNINSGVGLSSSSTMTNAQKSLTNSFSIDSQTPMTCVTQHPDKIYQCRHCLLRIHGYDLDSILFNCTKSVVIQLCRAPSEIAQVNWVKYLNQFIKTQSKSICDSGMSTPNTKPRSSKN 1268          
BLAST of Rho-associated protein kinase vs. Planmine SMEST
Match: SMESG000049701.1 (SMESG000049701.1)

HSP 1 Score: 309.686 bits (792), Expect = 7.395e-86
Identity = 168/409 (41.08%), Postives = 255/409 (62.35%), Query Frame = 3
Query:   66 NFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTN---FYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIM---MKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVC-FYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPD-DEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLK-STTFIGENIQFSGFSYIR 1265
            N+++ V D   +++L+ L DS    + +C+    LP +K   N   F K  K   +K+   R++ SDF+ +++IG+GAFG+V +VR K+ G+++A+K L+K  ++       +QEERD++   MKI S WI +LHYAFQD   LY VMD+  GG+++  +SK    L +++  FY AE+V++I+ +H  G++HRDIKPDN+L+D  GHI LADFGSCVK+ P    +   RA+GTPDYISPE+L S   +  YG E DWW+LG++++E+ YGE PFY+++   TY +IM  E     P + D+VS  A D +K+LIC +  RLG  K   D K+H FF    RE  WD ++   +PFIP + +  DTS F+F  DD + N     ++G   S+ F G ++   GF++ +
Sbjct:   21 NYKSSVPD---SVTLEFLIDSLFCTYYECR----LPFHKDEKNVAQFIKSIKGTVDKIESLRIRRSDFDTIRVIGRGAFGKVSVVRFKEKGEIYAMKSLHKYDILKKIDAACFQEERDVLFQAMKIKSPWITRLHYAFQDEFYLYFVMDFYNGGDMLTLLSKFNDRLPEEITKFYIAEVVMSIDVVHKLGYLHRDIKPDNLLLDSLGHIVLADFGSCVKLRPNGTTIT-ERAVGTPDYISPEILRSAEGHGPYGVECDWWSLGIVIHEMLYGELPFYNEDFSETYWQIMNFETCFQIPTEYDDVSDEAEDLIKQLICKKECRLG-NKGIDDFKNHPFF----REIDWDNLRSYNAPFIPEVSSAEDTSNFEF--DDFNAN-----HEGPPLSSNFKGCHLPCIGFTFTK 409          
BLAST of Rho-associated protein kinase vs. Planmine SMEST
Match: SMESG000049701.1 (SMESG000049701.1)

HSP 1 Score: 309.686 bits (792), Expect = 7.754e-86
Identity = 168/409 (41.08%), Postives = 255/409 (62.35%), Query Frame = 3
Query:   66 NFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTNKYFTN---FYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIM---MKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVC-FYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPD-DEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLK-STTFIGENIQFSGFSYIR 1265
            N+++ V D   +++L+ L DS    + +C+    LP +K   N   F K  K   +K+   R++ SDF+ +++IG+GAFG+V +VR K+ G+++A+K L+K  ++       +QEERD++   MKI S WI +LHYAFQD   LY VMD+  GG+++  +SK    L +++  FY AE+V++I+ +H  G++HRDIKPDN+L+D  GHI LADFGSCVK+ P    +   RA+GTPDYISPE+L S   +  YG E DWW+LG++++E+ YGE PFY+++   TY +IM  E     P + D+VS  A D +K+LIC +  RLG  K   D K+H FF    RE  WD ++   +PFIP + +  DTS F+F  DD + N     ++G   S+ F G ++   GF++ +
Sbjct:   21 NYKSSVPD---SVTLEFLIDSLFCTYYECR----LPFHKDEKNVAQFIKSIKGTVDKIESLRIRRSDFDTIRVIGRGAFGKVSVVRFKEKGEIYAMKSLHKYDILKKIDAACFQEERDVLFQAMKIKSPWITRLHYAFQDEFYLYFVMDFYNGGDMLTLLSKFNDRLPEEITKFYIAEVVMSIDVVHKLGYLHRDIKPDNLLLDSLGHIVLADFGSCVKLRPNGTTIT-ERAVGTPDYISPEILRSAEGHGPYGVECDWWSLGIVIHEMLYGELPFYNEDFSETYWQIMNFETCFQIPTEYDDVSDEAEDLIKQLICKKECRLG-NKGIDDFKNHPFF----REIDWDNLRSYNAPFIPEVSSAEDTSNFEF--DDFNAN-----HEGPPLSSNFKGCHLPCIGFTFTK 409          
BLAST of Rho-associated protein kinase vs. Planmine SMEST
Match: SMESG000057753.1 (SMESG000057753.1)

HSP 1 Score: 305.449 bits (781), Expect = 1.763e-85
Identity = 152/366 (41.53%), Postives = 226/366 (61.75%), Query Frame = 3
Query:  186 FTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLLSQS-DNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIGENIQFSGFSYIRWQNTN 1280
            F+NF        EK+   R+  SDF ++K +GQG FG+V LVRHK T +++A+KI++KS  V      FW EERDIM K  S W++K  Y F D K+LY+VM+Y+PGG+L +WM+++E   ++   FYA E+VLA+  LHN GF+HRD+KPDN+L+D  GH+KLADFG+C++       V  N  +GTPDYI PE+L +    + + G E DWWA G+++YE+FYGE PF  ++    Y  IM ++ +L +P D       +DFM K++ + + R+    D + IKSH +  NS  +W +  I++  SP I  +  + DT+Y  +  +D      N SN  + +  F GEN+ FSGF++ R+   N
Sbjct:   59 FSNFS-------EKIYKLRVSLSDFAVIKTVGQGGFGRVDLVRHKSTKNVYAMKIMDKSTFVNNSFNGFW-EERDIMAKNSSDWLIKCFYTFMDLKKLYIVMEYMPGGDLYHWMTELEDFSDEQTHFYAMELVLAVEVLHNMGFIHRDLKPDNMLLDKNGHMKLADFGTCIRTK--NGKVKTNFQVGTPDYICPEILRNTGIHDLEVGFETDWWAFGIVIYELFYGETPFEGESTSTLYQNIMRYKSTLIYPDDKPAKPEFIDFMSKILTEPTERM----DITGIKSHPYLTNSGYKWNFSDIREHQSPVIFEVSGEDDTNYITYDPND---RRNNISNNFINNNYFSGENLAFSGFTFNRFNTYN 407          
BLAST of Rho-associated protein kinase vs. Planmine SMEST
Match: SMESG000068357.1 (SMESG000068357.1)

HSP 1 Score: 245.358 bits (625), Expect = 6.429e-70
Identity = 138/390 (35.38%), Postives = 216/390 (55.38%), Query Frame = 3
Query:  198 YKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTGDLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRLYMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRDIKPDNILIDIKGHIKLADFGSCVKMDP---------VTKYVNCNRA------------------------IGTPDYISPEVLLSQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSLSFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWTWDTIQDSTSPFIPVLENDL-DTSYF-DFGEDDVDTN---FLNQSNQGLKSTTFIGENIQFSGF 1253
            + Q +  Y +L   R+   DF  L++IG+GAFG+V+LV+ K  G ++A+KIL K  ++  +     + ERDI+ K D+ W+VK+ ++FQD   LY+VM++LPGG+++  + K++ L E+   FY AE  LAI+ +H+ GF+HRDIKPDN+L+D KGHIKL+DFG C  +           +++   C+ A                        +GTPDYI+PEV   +     Y    DWW+LGV+++E+  G PPF SD    TY K+M     L FPPD  +S ++ + + + + + + RLG      +IK+  FF+       W+ I++  +  IPV+ N + DTS F DF + D+  N     ++ N+  K   FI  N  +  F
Sbjct:   78 HAQKESEYLRLKRARLVVDDFAALRVIGKGAFGEVRLVQKKDNGRIYAMKILRKVDMLQKDQIAHVRAERDILAKADNPWVVKMFFSFQDSTNLYLVMEFLPGGDMMTLLMKLDTLSEEQSQFYVAETALAIDSIHSMGFIHRDIKPDNLLLDAKGHIKLSDFGLCTGLKKSHRTEFYKDLSQAKPCDFAQSPLDSKRRAESWRKNRRNLAYSTVGTPDYIAPEVFQQKG----YTSSCDWWSLGVIMFEMLIGYPPFCSDTPGETYKKVMAFRDHLVFPPDTPISDVSKEVITEFLEEGNKRLGFNSGIVEIKNKSFFHGVD----WEHIRERPAA-IPVIVNSIDDTSNFDDFPDADLSWNINHIQDRENRYKKELAFI--NYTYKAF 456          
The following BLAST results are available for this feature:
BLAST of Rho-associated protein kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ROCK12.683e-11645.75Rho associated coiled-coil containing protein kina... [more]
ROCK19.611e-11445.75Rho associated coiled-coil containing protein kina... [more]
ROCK21.024e-11344.69Rho associated coiled-coil containing protein kina... [more]
ROCK26.466e-11344.69Rho associated coiled-coil containing protein kina... [more]
CDC42BPB3.056e-9440.78CDC42 binding protein kinase beta [Source:HGNC Sym... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
let-5026.057e-11345.75Rho-associated protein kinase let-502 [Source:Uni... [more]
mrck-14.548e-10044.99Serine/threonine-protein kinase mrck-1 [Source:Un... [more]
mrck-14.548e-10044.99Serine/threonine-protein kinase mrck-1 [Source:Un... [more]
mrck-13.193e-9944.61Serine/threonine-protein kinase mrck-1 [Source:Un... [more]
mrck-13.193e-9944.61Serine/threonine-protein kinase mrck-1 [Source:Un... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rok1.534e-10543.86gene:FBgn0026181 transcript:FBtr0340152[more]
Rok1.596e-10543.86gene:FBgn0026181 transcript:FBtr0340153[more]
Rok1.674e-10543.86gene:FBgn0026181 transcript:FBtr0340151[more]
Rok1.679e-10543.86gene:FBgn0026181 transcript:FBtr0074286[more]
gek4.004e-8642.46gene:FBgn0023081 transcript:FBtr0072287[more]
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BLAST of Rho-associated protein kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rock14.925e-11245.61Rho-associated, coiled-coil containing protein kin... [more]
rock2a1.599e-11044.47rho-associated, coiled-coil containing protein kin... [more]
rock2a1.691e-11044.08rho-associated, coiled-coil containing protein kin... [more]
rock2b1.158e-10745.36rho-associated, coiled-coil containing protein kin... [more]
rock2b1.238e-10745.36rho-associated, coiled-coil containing protein kin... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
med183.783e-11145.39mediator complex subunit 18 [Source:Xenbase;Acc:XB... [more]
med188.709e-11145.39mediator complex subunit 18 [Source:Xenbase;Acc:XB... [more]
med181.735e-11045.39mediator complex subunit 18 [Source:Xenbase;Acc:XB... [more]
med185.384e-11045.15mediator complex subunit 18 [Source:Xenbase;Acc:XB... [more]
ROCK12.962e-10946.85Rho-associated, coiled-coil containing protein kin... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rock14.730e-11644.42Rho-associated coiled-coil containing protein kina... [more]
Rock18.023e-11444.42Rho-associated coiled-coil containing protein kina... [more]
Rock21.439e-11144.44Rho-associated coiled-coil containing protein kina... [more]
Rock21.589e-11144.44Rho-associated coiled-coil containing protein kina... [more]
Rock18.189e-10948.26Rho-associated coiled-coil containing protein kina... [more]
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BLAST of Rho-associated protein kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O75116|ROCK2_HUMAN4.366e-11244.69Rho-associated protein kinase 2 OS=Homo sapiens OX... [more]
sp|A8WVU9|ROCK_CAEBR4.720e-11246.29Rho-associated protein kinase let-502 OS=Caenorhab... [more]
sp|P92199|ROCK_CAEEL7.705e-11245.75Rho-associated protein kinase let-502 OS=Caenorhab... [more]
sp|M3TYT0|ROCK2_PIG2.466e-11144.69Rho-associated protein kinase 2 OS=Sus scrofa OX=9... [more]
sp|P70336|ROCK2_MOUSE3.333e-11144.44Rho-associated protein kinase 2 OS=Mus musculus OX... [more]
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BLAST of Rho-associated protein kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q1HNI31.563e-11445.23Rho-associated, coiled-coil containing protein kin... [more]
A0A1S3JNX43.947e-11446.10rho-associated protein kinase 2-like isoform X2 OS... [more]
A0A0S7LGB85.353e-11445.72ROCK1 OS=Poeciliopsis prolifica OX=188132 GN=ROCK1... [more]
A0A0S7LGC51.011e-11345.72ROCK1 OS=Poeciliopsis prolifica OX=188132 GN=ROCK1... [more]
A0A0S7LGC97.647e-11345.79ROCK1 OS=Poeciliopsis prolifica OX=188132 GN=ROCK1... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rock2a7.389e-11145.00rho-associated protein kinase 2 [Source:NCBI gene;... [more]
ROCK12.539e-11048.08Rho associated coiled-coil containing protein kina... [more]
rock2a5.041e-11045.00rho-associated protein kinase 2 [Source:NCBI gene;... [more]
rock2b3.884e-10642.63rho-associated, coiled-coil containing protein kin... [more]
rock2a7.572e-10548.12rho-associated protein kinase 2 [Source:NCBI gene;... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000004595.12.116e-10745.45pep scaffold:Pmarinus_7.0:GL477044:11103:63873:-1 ... [more]
ENSPMAT00000003561.14.804e-10544.14pep scaffold:Pmarinus_7.0:GL478057:5187:43749:1 ge... [more]
ENSPMAT00000006413.19.943e-9646.86pep scaffold:Pmarinus_7.0:GL485553:3974:11158:-1 g... [more]
ENSPMAT00000002084.17.769e-9444.57pep scaffold:Pmarinus_7.0:GL476486:6827:40506:-1 g... [more]
ENSPMAT00000005559.11.601e-7736.77pep scaffold:Pmarinus_7.0:GL476474:6865:57025:1 ge... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
TPK39.807e-5838.89cAMP-dependent protein kinase catalytic subunit; p... [more]
TPK11.570e-5736.56cAMP-dependent protein kinase catalytic subunit; p... [more]
YPK26.617e-5732.88Protein kinase similar to S/T protein kinase Ypk1p... [more]
TPK29.043e-5736.42cAMP-dependent protein kinase catalytic subunit; p... [more]
YPK11.681e-5536.71S/T protein kinase; phosphorylates, downregulates ... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO376771.640e-8941.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO320081.760e-8649.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427006.756e-6935.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO360345.286e-6836.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO378001.612e-5735.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Rho-associated protein kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rock11.488e-11344.34Rho associated coiled-coil containing protein kina... [more]
rock13.717e-11146.32Rho associated coiled-coil containing protein kina... [more]
rock2a8.548e-10944.78rho-associated protein kinase 2 [Source:NCBI gene;... [more]
rock2a1.340e-10844.78rho-associated protein kinase 2 [Source:NCBI gene;... [more]
rock2a8.930e-10746.11rho-associated protein kinase 2 [Source:NCBI gene;... [more]
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BLAST of Rho-associated protein kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041847.10.000e+099.68SMESG000041847.1[more]
SMESG000049701.17.395e-8641.08SMESG000049701.1[more]
SMESG000049701.17.754e-8641.08SMESG000049701.1[more]
SMESG000057753.11.763e-8541.53SMESG000057753.1[more]
SMESG000068357.16.429e-7035.38SMESG000068357.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30031032 ID=SMED30031032|Name=Rho-associated protein kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=4011bp
TCTATATAAATCTTATATGATTCGACAAGATGGACAAGCTAAATAATTAT
GTATTATTGGAAAATAATTTTCAAAACGTAGTATCGGATGACAAAGAAAA
TATTTCACTCGATCTATTATTTGATTCATTTCAAAATTTGTTTCTTGATT
GTAAATCAGTATCAAATCTTCCAACGAATAAATATTTTACTAATTTTTAC
AAGCAGCATAAAAAAGTTTACGAGAAATTGGTTGATTTCCGTATGAAATA
CTCTGATTTTAATCTGCTGAAAATAATCGGCCAAGGAGCATTTGGTCAAG
TACAGTTAGTTAGGCATAAACAAACTGGAGATTTGTTTGCGTTAAAGATA
TTAAACAAGTCACAAGTTGTGACTTGTGAAGGTACCTTGTTTTGGCAAGA
GGAGCGTGACATAATGATGAAAATAGACAGTAGATGGATCGTCAAATTGC
ATTATGCATTTCAGGATCCGAAAAGATTGTATATGGTAATGGATTATTTA
CCCGGTGGAAATTTGGTCAATTGGATGAGTAAAGTGGAAGTTCTCCTGGA
GAAAGACGTGTGTTTTTATGCTGCAGAAATTGTCTTAGCAATAAATGGAC
TTCATAATTTCGGTTTTGTTCATCGAGACATAAAACCAGACAATATCCTC
ATTGACATTAAAGGCCATATAAAGTTAGCAGACTTTGGTTCCTGTGTAAA
AATGGATCCAGTCACAAAATATGTTAATTGTAATCGAGCTATCGGTACTC
CTGATTACATAAGTCCAGAAGTACTGTTGTCACAGAGTGATAACACAAAG
TACGGGAAAGAAGTCGATTGGTGGGCATTAGGAGTCATGCTGTATGAAAT
ATTTTACGGTGAACCGCCATTCTATTCAGACAATCTCATTAATACATATG
GCAAAATCATGACACATGAGCAAAGTCTTTCTTTTCCACCAGACGACGAA
GTATCAGGGATGGCTCTTGATTTTATGAAAAAATTGATATGTGATCGCTC
TGTTCGTCTTGGTCAAACTAAAGATGCCAGTGATATTAAATCACATATAT
TTTTCAACAACAGCTCTCGTGAATGGACCTGGGACACTATTCAAGATAGC
ACCTCTCCATTTATTCCGGTGCTAGAAAACGATTTAGATACATCGTATTT
TGATTTTGGAGAAGACGACGTAGACACGAATTTTTTAAACCAAAGCAACC
AAGGATTGAAATCTACAACGTTTATCGGTGAAAATATCCAGTTTTCAGGA
TTCTCATATATTCGATGGCAAAATACAAATAAGGAGAACAAAAAGTCGGA
TAACGTCCTCAATTCCAATATTATCAATGAAGATAAATTGCAATCCACTA
ATAGAGAGAATGAAATCAGTAAAGACATTCTGGATAAACTAATGATAGAT
AAAAAGGACCTGGAAAACCAGCTTCATGTTGTTAGTACCAATTTCAGAAA
AGAAGAGATTATTTCTAAAAAATTACAAATTGACTTTAGTGACTTACTGA
CACAACACTCTTGCTGCCAAAAGACAATTGAGGAACTTGAAACGATTCGA
GAAAAATTGAATTCAGATTTGGTAATACAACAGTCATCCTCTGAACAAGA
AAAAGAACTGCTAACCAAAGAGCTCACCGAAGTAAATGGGTATTTGAAAG
CTACCAATTTAAAACTCATGGAAGTTCAAACCAAATTCCATGAATCGAAA
TTGCGCTGTGTCCAACTGGAGAGTGATTATAATAATTTGATCGAATCTCA
TAATTTACTGGAGAAAGATTTGGTGAGACTAAGAAATTTTATTTCTTCAA
AAAAGGATGAAGAATCATCAATCAACGATAGCGATTTCATTCAACTCAAG
TCTGAATATGAGTCGAAAGACTATGATTTACAGAAAAGTCTATTGGAAAA
CAAATGCATCGCAAAGTCTTTGGAAAGCCTTCAAAATGAAAAAGAAAAGC
TATCGTATTCCCTTATTGAATTAGAAGCTAAATTTACCGAACAACTCCAT
GAAAATAGAAGGCAAGAAACAGAACTGAAAGAGAAGGAAGACTTGATTAA
AGAAATGAAAGATGAATTAAGTCGATTGAAAGTCTCCATTGACGAGTCAT
TAAAGAAAGAACAAAATACTCATGTGAAATATAGTAATCTAATGAAGTTA
CATACGCAAGAGAAAAACAAATTTGAACAGGAGCTAGAAGAACAACGGAA
CAAACTTAGCAAAATTATCACAGACAAGACTTCAGAAATTAAACTTATAA
ACAGTTCGGTGGAAACTATGAAAAATTCGAACACGAAATTACTGAAAGAT
CTAGAAATGGCAAAGGTCAAACAGGAGTCGCTGGTAGAGAAGATTAAAGA
TATTATACATGGACAAAGTACGACGGCCAAAGAAATGATGATATCTGAAG
ATCTTAAAAGCGGTTTCCTGGGAAAAAGATCTGGAAGAATTTATCACCAA
TTAGCGAAAGAAAACAAAAGATTGGAGAACCTATTGGATGAGTTTAAAAA
AACTTCGCAGTGTAAAGAAAAGGAACTTATGAAACAAGTGGAAACTATCA
CGAGAGAGAAATGCCAATTAGAGCTGGAATTAAGCAGTTCACAAGATGGA
TATTATGAAAATCTGAATAGCCACGATATTTCTTGCAATCGAGCCAGCGT
TAGTACTATTGCTTCATCTCACAGCAGTTTTGATAGTGGATTTCCTCACG
ACTCCTCACTTGTTTCCCAATCTAATTTCCGCCGTTCTATCAAAAATCCA
TTCTCAAAGCCTCCATTATTATCTCACAAAGCAAGCCACTTGAAGCAAGA
TTTAACAAATGACGTTTTCCCCGAAAGTGGCGACGAAAATAATGGAGTAA
AACTTCGTCAGAAAAAGTCGACCAATTTACAAAATAGGAATTCTAAAACT
GAAAGCATGACGATGGATTTTCGGAATATTAATCAAATTTTAGAAGTCAA
TGTGAAAAACAAAGGCAAAAAAATGTCCTGGGAAAAACGTTTTGTTCGAC
TTGAAGAACAGAATCTTTGCTTCTATGGGGAAGATGTCCAAACCTGTCAT
TTTCGATTATTACTTTCAAGCTTGATTTTCGTTCGATCAGTTTCCAATGT
CGACGTAATACACGCCAAATCATCGGAAATACCGCGGATATTTCAAATTT
GTTACGACAAAAATGATTCTCTTTATCTAACTGCTCAGATGAATCCAATC
AGCAAATCTACCACTTCTATAAATAGCGCGGAATCTCTAAAGAGCAGTAA
CTCAAGTAATGAGAATGAAGTCAATGTTACCGTTACAGAGAAAAAAGATT
TTCGGAAAACTAGTCTGGCTAGGTTTCTGCCGATTAAAGGTTTCAAAAGT
AATAAAAATAAAGAAAATCCAGAATTACCAAACAATTTATCCAATAACTT
TCCGGTGTTCTTGAAAGGCCACATTTTTACAATAATAACCACTGAAACGA
GATCGAGTTGTTTAATCTGCAACAAAACTTTCAACATCAACAGTGGTGTT
GGCCTCTCATCATCATCAACCATGACCAATGCCCAGAAATCTTTAACGAA
CTCCTTCTCCATAGACTCTCAAACCCCCATGACATGTGTTACTCAACATC
CAGATAAGATCTACCAATGTCGACATTGCCTGTTGAGAATTCACGGCTAT
GATTTGGATTCTATTCTGTTCAATTGCACCAAATCAGTGGTCATTCAGTT
GTGTCGAGCACCTTCTGAAATCGCACAAGTCAATTGGGTGAAATATCTGA
ACCAATTCATCAAAACCCAATCGAAATCGATTTGTGATTCCGGAATGTCA
ACGCCAAATACAAAACCTCGATCTTCAAAGAACTAGCTCAAGAAATATGT
CTGAACTGACAATTAGATTTATTTCTGAATAGATTTATGTATTTTTGCTT
TCATGTTTATATGGATTACAATCTCCTCTCTAATGTTTGTCTGTAGATAT
ATATATTCCTTGAGATTTGTGAGAGTTCTTGACTGCTTGTAGATCCCCTG
TATTATTATTG
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protein sequence of SMED30031032-orf-1

>SMED30031032-orf-1 ID=SMED30031032-orf-1|Name=SMED30031032-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1269bp
MDKLNNYVLLENNFQNVVSDDKENISLDLLFDSFQNLFLDCKSVSNLPTN
KYFTNFYKQHKKVYEKLVDFRMKYSDFNLLKIIGQGAFGQVQLVRHKQTG
DLFALKILNKSQVVTCEGTLFWQEERDIMMKIDSRWIVKLHYAFQDPKRL
YMVMDYLPGGNLVNWMSKVEVLLEKDVCFYAAEIVLAINGLHNFGFVHRD
IKPDNILIDIKGHIKLADFGSCVKMDPVTKYVNCNRAIGTPDYISPEVLL
SQSDNTKYGKEVDWWALGVMLYEIFYGEPPFYSDNLINTYGKIMTHEQSL
SFPPDDEVSGMALDFMKKLICDRSVRLGQTKDASDIKSHIFFNNSSREWT
WDTIQDSTSPFIPVLENDLDTSYFDFGEDDVDTNFLNQSNQGLKSTTFIG
ENIQFSGFSYIRWQNTNKENKKSDNVLNSNIINEDKLQSTNRENEISKDI
LDKLMIDKKDLENQLHVVSTNFRKEEIISKKLQIDFSDLLTQHSCCQKTI
EELETIREKLNSDLVIQQSSSEQEKELLTKELTEVNGYLKATNLKLMEVQ
TKFHESKLRCVQLESDYNNLIESHNLLEKDLVRLRNFISSKKDEESSIND
SDFIQLKSEYESKDYDLQKSLLENKCIAKSLESLQNEKEKLSYSLIELEA
KFTEQLHENRRQETELKEKEDLIKEMKDELSRLKVSIDESLKKEQNTHVK
YSNLMKLHTQEKNKFEQELEEQRNKLSKIITDKTSEIKLINSSVETMKNS
NTKLLKDLEMAKVKQESLVEKIKDIIHGQSTTAKEMMISEDLKSGFLGKR
SGRIYHQLAKENKRLENLLDEFKKTSQCKEKELMKQVETITREKCQLELE
LSSSQDGYYENLNSHDISCNRASVSTIASSHSSFDSGFPHDSSLVSQSNF
RRSIKNPFSKPPLLSHKASHLKQDLTNDVFPESGDENNGVKLRQKKSTNL
QNRNSKTESMTMDFRNINQILEVNVKNKGKKMSWEKRFVRLEEQNLCFYG
EDVQTCHFRLLLSSLIFVRSVSNVDVIHAKSSEIPRIFQICYDKNDSLYL
TAQMNPISKSTTSINSAESLKSSNSSNENEVNVTVTEKKDFRKTSLARFL
PIKGFKSNKNKENPELPNNLSNNFPVFLKGHIFTIITTETRSSCLICNKT
FNINSGVGLSSSSTMTNAQKSLTNSFSIDSQTPMTCVTQHPDKIYQCRHC
LLRIHGYDLDSILFNCTKSVVIQLCRAPSEIAQVNWVKYLNQFIKTQSKS
ICDSGMSTPNTKPRSSKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0003347epicardial cell to mesenchymal cell transition
GO:0051496positive regulation of stress fiber assembly
GO:1903392negative regulation of adherens junction organization
GO:0006468protein phosphorylation
GO:0006915apoptotic process
GO:0007266Rho protein signal transduction
GO:0016310phosphorylation
GO:0030036actin cytoskeleton organization
GO:0035556intracellular signal transduction
GO:0051451myoblast migration
GO:0051492regulation of stress fiber assembly
GO:0051493regulation of cytoskeleton organization
GO:0051894positive regulation of focal adhesion assembly
GO:2000114regulation of establishment of cell polarity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000101muscle cell
PLANA:0000140anterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR029875LET-502
IPR000961AGC-kinase_C
IPR000719Prot_kinase_dom
IPR011993PH-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR017441Protein_kinase_ATP_BS
IPR011009Kinase-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0004674protein serine/threonine kinase activity
GO:0004672protein kinase activity
GO:0000166nucleotide binding
GO:0016301kinase activity
GO:0016740transferase activity
GO:0017048Rho GTPase binding
GO:0017049GTP-Rho binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0000139Golgi membrane
GO:0001726ruffle
GO:0005737cytoplasm
GO:0005794Golgi apparatus
GO:0005814centriole
GO:0005856cytoskeleton
GO:0005886plasma membrane
GO:0016020membrane
GO:0030027lamellipodium
GO:0032059bleb
GO:0042995cell projection
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 808..835
NoneNo IPR availableCOILSCoilCoilcoord: 662..696
NoneNo IPR availableCOILSCoilCoilcoord: 563..583
NoneNo IPR availableCOILSCoilCoilcoord: 447..474
NoneNo IPR availableCOILSCoilCoilcoord: 634..654
NoneNo IPR availableCOILSCoilCoilcoord: 708..739
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 164..367
e-value: 2.3E-140
score: 469.7
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 31..413
e-value: 2.3E-140
score: 469.7
NoneNo IPR availablePANTHERPTHR22988MYOTONIC DYSTROPHY S/T KINASE-RELATEDcoord: 14..852
NoneNo IPR availablePANTHERPTHR22988MYOTONIC DYSTROPHY S/T KINASE-RELATEDcoord: 423..1249
IPR000961AGC-kinase, C-terminalSMARTSM00133pkinase_C_6coord: 350..415
e-value: 0.007
score: 24.2
IPR000961AGC-kinase, C-terminalPROSITEPS51285AGC_KINASE_CTERcoord: 350..423
score: 11.554
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 80..345
e-value: 4.8E-86
score: 301.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 81..345
e-value: 7.0E-61
score: 205.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 80..345
score: 44.513
IPR011993PH-like domain superfamilyGENE3DG3DSA:2.30.29.30coord: 970..1076
e-value: 5.3E-12
score: 48.1
IPR029875Rho-associated protein kinase LET-502PANTHERPTHR22988:SF38RHO-ASSOCIATED PROTEIN KINASEcoord: 423..1249
IPR029875Rho-associated protein kinase LET-502PANTHERPTHR22988:SF38RHO-ASSOCIATED PROTEIN KINASEcoord: 14..852
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 199..211
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 86..109
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 77..399