SMED30030974
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030974 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11Note: Hover over icons to view figure legend
Alignments
SMED30030974 aligns in the following genomic locations:
Homology
BLAST of SMED30030974 vs. Planmine SMEST
Match: SMESG000005752.1 (SMESG000005752.1) HSP 1 Score: 338.191 bits (866), Expect = 1.302e-107 Identity = 179/185 (96.76%), Postives = 182/185 (98.38%), Query Frame = 3 Query: 3 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVEKVDESEIIDIAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTETNIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKMESVFETELIEEYPQSLEASNVE 557 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVEKVDESEIIDIAEIKQTDSTTTVVEN LEIQ+IDEKADKPEINILTETNIQSE+EMETALEHTENVNVEADFKTKDNEELQIVSE VEIVDEHETETCKEEFYEAP+ENKMESV ETELIEEYPQSLEASNVE Sbjct: 7205 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVEKVDESEIIDIAEIKQTDSTTTVVENFLEIQVIDEKADKPEINILTETNIQSEVEMETALEHTENVNVEADFKTKDNEELQIVSENVEIVDEHETETCKEEFYEAPLENKMESVIETELIEEYPQSLEASNVE 7389 HSP 2 Score: 140.969 bits (354), Expect = 9.583e-39 Identity = 95/206 (46.12%), Postives = 133/206 (64.56%), Query Frame = 3 Query: 12 VLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVL--ISESVEKVDESEIID-IAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTET---------------------NIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKMESVFETELIEEYPQSLEASNVE 557 V+ET +I+E + LE ++I EEE+N++E +KP+ L E +E+V++ EI D EIKQ ++ T V EN E ++ + K++KPEIN+ E +I+ E+E L E+ NVEADFKTKDNEELQIVSE VEIVDEHE +TC+EE Y+ P+E+K+E+VFET++IE+YPQ+LEASNVE Sbjct: 7370 VIETELIEEYPQSLEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKVFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEESIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENVEIVDEHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVE 7575 HSP 3 Score: 116.316 bits (290), Expect = 4.525e-30 Identity = 89/188 (47.34%), Postives = 130/188 (69.15%), Query Frame = 3 Query: 3 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVEKVDESE--IIDIAEIKQTDSTTTVVENVLEIQIIDEKADKPEINIL----TETNIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKM-ESVFETELIEEYPQSLEA 545 E +V +T +IKEQ EFLE LV +NI EEEM++VE VK E+ TVL +E+VE++ E E +++EIKQ ++ V+E+VLE I + + + +I ++ E +I+ E+E L EN +VEA+FKTKDN+ELQI+SE VE DEH+TET +EF E +E+K E+VF+T++IEE+PQ +EA Sbjct: 8614 EENVFKTEIIKEQPEFLEALVAENIVEEEMHNVEGTLVKTEKQQTVL-AEAVEQIKELEHDKDEVSEIKQALVSSAVMEDVLETNIHESEVKELDIKVIDSIEREESIEDSFEIEMELSDVENEDVEAEFKTKDNKELQILSENVENHDEHQTETF-QEFDEVNLESKAEENVFKTQVIEEHPQFVEA 8799 HSP 4 Score: 109.383 bits (272), Expect = 9.389e-28 Identity = 80/206 (38.83%), Postives = 124/206 (60.19%), Query Frame = 3 Query: 3 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVE---KVDESEIID-IAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTET---------------------NIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKM-ESVFETELIEEYPQSLE 542 E +V +T+VI+E +F+E L+TDNI +E +N V ++SE VE K+DE+E+ + I EIK + T++++N+LE ++ + K++ PE+N+L E +I+ E+E L E V+ADF+TKDN+ELQ++SE EI +EHETE CKEE YE +E+KM E+ +++IEE+PQ LE Sbjct: 8781 EENVFKTQVIEEHPQFVEALITDNIEDEVVNDV------------AVLSEGVEQTQKLDENEVKEEIPEIKPITALTSLIKNILEDKVCEHKSEMPEVNVLEEVKTEMEVVETEDVIKEIIEKQESIEGSFEIEMELSDIEREIVKADFETKDNKELQVLSENAEIQEEHETEKCKEELYEVNLESKMEENASMSKVIEEHPQILE 8974 HSP 5 Score: 107.071 bits (266), Expect = 7.537e-27 Identity = 78/188 (41.49%), Postives = 110/188 (58.51%), Query Frame = 3 Query: 9 DVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVL--ISESVEKVDESEIID-IAEIKQTDSTTTVVENVLEIQIIDEKADKPEINIL---------------------TETNIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKMESV 500 +V ET+VI++ + LE ++I EEE+N++E +KP+ L E +E+V++ EI D EIKQ ++ T V EN E + + K++KPEIN+ E +I+ E+E L E+ NVEADFKTKDNEELQIVSE +EIVDEHE +TC EE YE + +K+E V Sbjct: 7555 NVFETKVIEQYPQALEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEDSIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENIEIVDEHEAKTCGEELYEKSLTSKVEDV 7742 HSP 6 Score: 98.9821 bits (245), Expect = 4.156e-24 Identity = 79/204 (38.73%), Postives = 119/204 (58.33%), Query Frame = 3 Query: 3 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVEK---VDESEIIDIAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTETNIQSEI--------------------EMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKM-ESVFETELIEEYPQSLE 542 E ++ ET+VI+E +FLE + +I EEE SVE VKPE + ++SE++E+ + +SEI +I+EIK ++ V++N E ++ K + ++ IL E+N Q E+ EME L E+ NVEA+FKTKD+E+ +IVS +E VDEHE E KE E +ENK+ E+VF+ + IE+ P LE Sbjct: 8071 EQNLFETQVIEEIPQFLETTIISDIVEEETKSVEGTIVKPE-VKMEILSEAIEQTQNLKQSEINEISEIKPNAASVLVIDNSPEENVLGLKPETFDVPILEESNKQDEVTEMEVKGNIVVNAESLEDSFEMEMELLDVESANVEAEFKTKDSEDFKIVSGCIETVDEHEAEAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLE 8273 HSP 7 Score: 96.2857 bits (238), Expect = 4.073e-23 Identity = 81/206 (39.32%), Postives = 122/206 (59.22%), Query Frame = 3 Query: 3 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVE---KVDESEII-DIAEIKQTDSTTTVVENVLEIQIIDEKADKPEINIL--------------------TETNIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKM-ESVFETELIEEYPQSLEA 545 E++V +T I E + LE + T+NI E+E+N++E VK E+ + L SE+VE K+++ + DI EI +T ++T ++EN LE K++K ++N+L E +I+ E+E L EN +VEADFKTKDN+ELQ VSE +EI DEHE E E I++K+ E+VF+TE+I+E P+ LEA Sbjct: 8433 EDNVYDTIAIVEHPQTLEAMATENIIEQEINTIEATIVKTEKKLSTL-SEAVEQNVKIEQGGVNEDILEIIETRASTALIENSLE-----NKSEKLDVNVLENSKVEETMKDEAVFEDIFVQEESIEDSFEIEMELSDLENEDVEADFKTKDNKELQNVSENLEITDEHEANIHDEALEEVSIQHKLEENVFKTEIIKEQPEFLEA 8632 HSP 8 Score: 94.7449 bits (234), Expect = 1.370e-22 Identity = 74/197 (37.56%), Postives = 123/197 (62.44%), Query Frame = 3 Query: 3 ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVE---KVDESEI-IDIAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTET-----------NIQSEIEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKME-SVFETELIEEYPQSLEA 545 E +V + + I++Q FLE LVT+NI EEE+ +++ + V + + VL SE+VE K ++ EI D+AEI+Q ++ +VV V+E + I+ +++KP++N+L E+ +I+ E+E L EN +EADFK K++ LQ VSE VE + ++ +EE E +EN++E +V++T I E+PQ+LEA Sbjct: 8256 EENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVL-SEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLEESKIETEITEKEESIEDSFEIEIELSDAENQELEADFKAKESLALQNVSENVENICDYAGHLSEEELNEVYLENEVEDNVYDTIAIVEHPQTLEA 8451 HSP 9 Score: 75.8702 bits (185), Expect = 4.453e-16 Identity = 71/206 (34.47%), Postives = 113/206 (54.85%), Query Frame = 3 Query: 9 DVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVEKVD---ESEIID-IAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTETNIQSE---------------------IEMETALEHTENVNVEADFKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKME-SVFETELIEEYPQSLEAS 548 +V +T +I+E +FLE L D + EEM +E + V P+E+ VL SE+VE+++ +SEI D +A I+Q V+ NV+E Q+++ K ++ + I ET I+ E E+E L +N +V+ +FK KD E SE +EIV E + ET E E + +++E ++FET++IEE PQ LE + Sbjct: 7890 NVFKTEIIQEHPQFLESLTNDTMAGEEMRILEAIIVAPKEVIKVL-SEAVEQIETIEQSEIQDDLANIEQD----IVLANVIEAQVLETKLEQADEYISMETKIEIETPNPEATTEETIEKEESIDDSFEIEMELSEVKNEDVKEEFKMKDKVEHHGASENIEIVHEQKAETSGETMDEVCLASRVEQNLFETQVIEEIPQFLETT 8090 HSP 10 Score: 73.9442 bits (180), Expect = 1.928e-15 Identity = 64/165 (38.79%), Postives = 100/165 (60.61%), Query Frame = 3 Query: 96 SVELVTVKPEELTTVLISESVE---KVDESEIID-IAEIKQTDSTTTVVENVLEIQIIDEKADKPEINILTETNIQSEIE-----METALEHTENVNVEAD-----FKTKDNEELQIVSETVEIVDEHETETCKEEFYEAPIENKME-SVFETELIEEYPQSLEA 545 S E + +KP+++ +L SESVE KV++ EI D AE+ Q + V+ENVL+++ + K KPE +IL N+Q EI ME ++E E++ V + KT+ N +LQ VSE +E +++H T +E E +EN E +VF+TE+I+E+PQ LE+ Sbjct: 7743 STEAIIIKPDDMKNIL-SESVEQIEKVEQCEIKDEFAEVMQDKAFEAVLENVLDVKATEPKTTKPERSILEIANVQVEIMKDESFMEESIEKEESLQVSFESENEYAKTESNVDLQNVSEDIENLEQHATNIYEERLDEVQLENVTELNVFKTEIIQEHPQFLES 7906 The following BLAST results are available for this feature:
BLAST of SMED30030974 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030974 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030974 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030974 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030974 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030974 ID=SMED30030974|Name=SMED30030974|organism=Schmidtea mediterranea sexual|type=transcript|length=558bpback to top protein sequence of SMED30030974-orf-1 >SMED30030974-orf-1 ID=SMED30030974-orf-1|Name=SMED30030974-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=185bp ENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISEback to top Annotated Terms
The following terms have been associated with this transcript:
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