Dimer_Tnp_hAT domain-containing protein

Overview
NameDimer_Tnp_hAT domain-containing protein
Smed IDSMED30030953
Length (bp)1177
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Dimer_Tnp_hAT domain-containing protein (SMED30030953) t-SNE clustered cells

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30030953) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dimer_Tnp_hAT domain-containing protein (SMED30030953) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30030953) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030953

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30030953 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30030953 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030953 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030953 dd_Smed_v4_17002_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000043280.1 (uncharacterized LOC100492546 [Source:NCBI gene;Acc:100492546])

HSP 1 Score: 90.1225 bits (222), Expect = 5.764e-19
Identity = 65/257 (25.29%), Postives = 131/257 (50.97%), Query Frame = 1 
Query:   64 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCM-----NCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVD--DVKCFDQFYNLKVFVEKNINIE-NFRKLITCLKWTE---YFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKV 801
            FA+S + +F E + ++E+++ + +E     N L   +  R  E+F P KVK+ILS   ++G       + + C      + Y  + +YL  W+    + + +  M L  +P  +  E+ +    SK+ +VD  +   F++   LK FV K+   E N   + TC +W++   +F   ++ F ++L  I +    +PG +A ++++FS ++     + +KL + +++ +L V+ N+  +SC EF  E+   +  L K+
Sbjct:  376 FANSSLCLFQETVNKVENQEGTAIESSVIMNDLIQKITVRRNENFIPPKVKTILSSMEEHG------SYTIACFFKVSNDFYTTALQYLNAWSVNFEHVDKMECMLLKHLPDRQSFEHAVHFFLSKSNLVDIKEDDLFNEITCLKSFVTKDKVQEWNSNGVTTCERWSQVFSHFSQKSLSF-TQLKTIVELTLCMPGSSAPIQKVFSAMNMMSDADYSKLGVQSLKSVLMVLINFP-MSCQEFAVELAYRKDILQKI 624
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A0F8AZA3 (Dimer_Tnp_hAT domain-containing protein OS=Larimichthys crocea OX=215358 GN=EH28_05728 PE=4 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.129e-63
Identity = 105/250 (42.00%), Postives = 153/250 (61.20%), Query Frame = 1 
Query:   73 SLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYF-KTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKY 819
            SLM +FH  I  +E E NS++EV    +S+ N L  R E +F  +KVK +L EN ++GLE    KF  + +  Y    +YL+ W + +  F    WM +   PTW+++E  I  L  K + +DDVKCFD+  NLK FVE   +   F  L    KW +YF K  ++  +SELLKI QF FA+P  NANVER+FS++  QWTKERN+L + +++GIL + YN+K++SC +F+  +  N+  L K+S+  KY
Sbjct:   37 SLMSVFHNHIQGMERENNSIMEVKKILSSIHNILTERKENTFMSLKVKGLLDENRRDGLEEGCVKFTDDVLGMYTSCVQYLEKWMTPMEEFSPFMWMDMSEPPTWDDVEACIKYLGEKGVPIDDVKCFDEVVNLKRFVESRGDDNEFMGLQVHQKWAKYFEKAKSIAAYSELLKIAQFVFALPAHNANVERVFSLMQSQWTKERNQLSVQSLKGILFLQYNFKDMSCKDFHAHMLSNKKVLRKISSTAKY 286
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A498MPB9 (Ac transposable element-derived 4 OS=Labeo rohita OX=84645 GN=ROHU_036788 PE=4 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 2.661e-61
Identity = 106/250 (42.40%), Postives = 153/250 (61.20%), Query Frame = 1 
Query:   73 SLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYF-KTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKY 819
            SLM +FHE I ++E E NSV+EV    +S+ N    R E +F  IKVK +L++  K+G + + ++F       Y    +YL  W + ++ F    WM L   P W ++E  I  L  K + +DDVKCFDQF NLK F E++ +   F+       WTEYF +  +V+ HSELLKI QF FA+P  NANVER+FS++  QWTKERN+L + +V+G+L V YN+KNI+C +F+  +  +   L K+S+  KY
Sbjct:  115 SLMTVFHEHIEDMEKENNSVVEVSNILHSVHNVFQERKENNFMSIKVKKLLAQKRKDGHDQDCNQFCAAVNGLYTACLQYLDKWMTPMAEFSPFMWMDLSETPDWNDVETCIKYLREKGVQIDDVKCFDQFTNLKKFAERSNSDGEFKGKQVHRMWTEYFERAKSVQNHSELLKIAQFVFALPSHNANVERVFSLMQSQWTKERNQLSVDSVKGLLLVQYNFKNITCKDFHAYLLNDRKLLGKISSSAKY 364
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A498P415 (Golgin subfamily A member 6C OS=Labeo rohita OX=84645 GN=ROHU_000925 PE=4 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 1.161e-59
Identity = 107/250 (42.80%), Postives = 152/250 (60.80%), Query Frame = 1 
Query:   73 SLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYF-KTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKY 819
            SLM +FHE I ++E E NSV+EV    +S+ N L  R E +F  IKVK +L++  K+G + + ++F       Y    +YL  W + ++ F    WM L   P W ++E  I  L  K +  DDVKCFDQF NLK F E++ +   F+       WTEYF +   V+ HSELLKI QF FA+P  NANVER+FS++  QWTKERN+L + +V+G+L V YN+KNI+C +F+  +  +   L K+S+  KY
Sbjct:  360 SLMIVFHEHIEDMEKENNSVVEVSNILHSVHNVLQERKENNFMSIKVKELLAQKRKDGHDQDCNQFCAAVNGLYTACLQYLDKWMTPMAEFSPFMWMDLSETPDWNDVETCIKYLREKGVQTDDVKCFDQFTNLKKFAERSNSDGEFKGKQVHRMWTEYFERAKTVQNHSELLKIAQFVFALPSHNANVERVFSLMQSQWTKERNQLSVDSVKGLLLVQYNFKNITCKDFHAYLLNDRKLLGKISSSAKY 609
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A498M8V7 (Galectin-3-binding A-like protein OS=Labeo rohita OX=84645 GN=ROHU_037089 PE=4 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 5.891e-58
Identity = 107/250 (42.80%), Postives = 152/250 (60.80%), Query Frame = 1 
Query:   73 SLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYF-KTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKY 819
            SLM +FHE I ++E E NSV+EV    +S+ N L  R E +F  IKVK +L++  K+G + + ++F       Y    +YL  W + ++ F    WM L   P W ++E  I  L  K +  DDVKCFDQF NLK F E++ +   F+       WTEYF +   V+ HSELLKI QF FA+P  NANVER+FS++  QWTKERN+L + +V+G+L V YN+KNI+C +F+  +  +   L K+S+  KY
Sbjct:  360 SLMIVFHEHIEDMEKENNSVVEVSNILHSVHNVLQERKENNFMSIKVKELLAQKRKDGHDQDCNQFCAAVNGLYTACLQYLDKWMTPMAEFSPFMWMDLSETPDWNDVETCIKYLREKGVQTDDVKCFDQFTNLKKFAERSNSDGEFKGKQVHRMWTEYFERAKTVQNHSELLKIAQFVFALPSHNANVERVFSLMQSQWTKERNQLSVDSVKGLLLVQYNFKNITCKDFHAYLLNDRKLLGKISSSAKY 609
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A498LZK3 (Ac transposable element-derived 4 OS=Labeo rohita OX=84645 GN=ROHU_009932 PE=4 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 2.821e-49
Identity = 83/201 (41.29%), Postives = 123/201 (61.19%), Query Frame = 1 
Query:  220 ILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYF-KTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKY 819
            +L++  K+G + + ++F       Y    +YL  W + ++ F    WM L   P W ++E  I  L  K + +DDVKCFDQF NLK F E++ +   F+       WTEYF +  +V+ HSELLKI QF FA+P  NANVER+FS++  QWTKERN+L + +V+G+L V YN+KNI+C +F+  +  +   L K+S+  KY
Sbjct:    1 MLAQKRKDGHDQDCNQFCAAVNGLYTACLQYLDKWMTPMAEFSPFMWMDLSETPDWNDVETCIKYLREKGVQIDDVKCFDQFTNLKKFAERSNSDGEFKGKQVHQMWTEYFERAKSVQNHSELLKIAQFVFALPSHNANVERVFSLMQSQWTKERNQLSVDSVKGLLLVQYNFKNITCKDFHAYLLNDRKLLGKISSSAKY 201
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000041838.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:29525919:29530293:-1 gene:ENSAMXG00000031975.1 transcript:ENSAMXT00000041838.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.7886 bits (177), Expect = 1.518e-13
Identity = 64/259 (24.71%), Postives = 120/259 (46.33%), Query Frame = 1 
Query:   61 VFADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSN--FESVTWMKLDRVPTWEEIENTI-VLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEK 828
             F ++++ IFH+ +L +E +  +V E+     +L   L  R  + FF ++  +IL +            F+    N YK +  YL+ W     N   ++V  + L    T+ ++ + + VL     + +D++    + Y + +  ++ I +E  RK     KW    K+        L  +  F F+IP  NA+VER+FS+++  WT +RN+  +  ++  + V  N++  SC EFY    + +  L    +  KY  K
Sbjct:  363 AFLNNILKIFHDVVLLLEGQDGTVCELYDIMLTLKTKLQQRQIDCFFGMETSAILQQFPDQKAATIKHDFS----NFYKTALNYLEKWYDFTDNNYQKNVRCLALKSSFTFSQLSDAVEVLQIGSKLDMDELY---EEYCVTLPRQQEI-VE--RKAPVVEKWALLLKSTKTP---NLTAVASFLFSIPITNASVERVFSLVTAAWTDQRNRCSVELIKSEIQVKTNFE-YSCKEFYSFALKQKALLEAARSSKKYKAK 607
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000048878.1 (pep primary_assembly:Astyanax_mexicanus-2.0:8:18990267:18996294:-1 gene:ENSAMXG00000041866.1 transcript:ENSAMXT00000048878.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 9.700e-9
Identity = 56/226 (24.78%), Postives = 101/226 (44.69%), Query Frame = 1 
Query:  160 LANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSN--FESVTWMKLDRVPTWEEIENTI-VLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEK 828
            L   L  R  + FF ++  +IL +            F+    N YK +  YL+ W     N   ++V  + L    T+ ++ + + VL     + +D++    + Y + +  ++ I +E  RK     KW    K+        L  +  F F+IP  NA+VER+FS+++  WT +RN+  +  ++  + V  N++  SC EFY    + +  L    +  KY  K
Sbjct:  359 LQTKLQQRQIDCFFGMETSAILQQFPDQKAATIKHDFS----NFYKTALNYLEKWYDFTDNNYQKNVRCLALKSSFTFSQLSDAVEVLQIGSKLDMDELY---EEYCVTLPRQQEI-VE--RKAPVVEKWALLLKSTKT---PNLTAVASFLFSIPITNASVERVFSLVTAAWTDQRNRCSVELIKSEIQVKTNFE-YSCKEFYSFALKQKALLEAARSSKKYKAK 570
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000079481.1 (SMESG000079481.1)

HSP 1 Score: 518.85 bits (1335), Expect = 0.000e+0
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 1 
Query:   64 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 831
            FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSE LKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS
Sbjct:  371 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSEFLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 626
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000023292.1 (SMESG000023292.1)

HSP 1 Score: 516.153 bits (1328), Expect = 2.853e-180
Identity = 254/256 (99.22%), Postives = 255/256 (99.61%), Query Frame = 1 
Query:   64 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 831
            FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEK+INIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENE FLNKVSNVDKYIEKS
Sbjct:  371 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKSINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENENFLNKVSNVDKYIEKS 626
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000052607.1 (SMESG000052607.1)

HSP 1 Score: 513.842 bits (1322), Expect = 2.163e-179
Identity = 252/256 (98.44%), Postives = 254/256 (99.22%), Query Frame = 1 
Query:   64 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 831
            FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENE DKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNIN+ENFRKLITCLKWTEYFKTNAVEFHSELLKIC+FFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGIL VVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS
Sbjct:  371 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEKDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINLENFRKLITCLKWTEYFKTNAVEFHSELLKICKFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILRVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 626
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000055041.1 (SMESG000055041.1)

HSP 1 Score: 465.692 bits (1197), Expect = 2.286e-166
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1 
Query:  142 MATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 831
            MATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS
Sbjct:    1 MATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 230
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000036787.1 (SMESG000036787.1)

HSP 1 Score: 414.846 bits (1065), Expect = 4.590e-140
Identity = 213/256 (83.20%), Postives = 215/256 (83.98%), Query Frame = 1 
Query:   64 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILSENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 831
            FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFF                                         ++QLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS
Sbjct:  458 FADSLMHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFF----------------------------------------QSNQLSNFESVTWMKLDRVPTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLITCLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKERNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEKS 673

HSP 2 Score: 48.1358 bits (113), Expect = 9.233e-6
Identity = 21/21 (100.00%), Postives = 21/21 (100.00%), Query Frame = 3 
Query:    3 PNMSKRKCLFTEELQAKFVCF 65
            PNMSKRKCLFTEELQAKFVCF
Sbjct:   86 PNMSKRKCLFTEELQAKFVCF 106
The following BLAST results are available for this feature:
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000043280.15.764e-1925.29uncharacterized LOC100492546 [Source:NCBI gene;Acc... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0F8AZA31.129e-6342.00Dimer_Tnp_hAT domain-containing protein OS=Larimic... [more]
A0A498MPB92.661e-6142.40Ac transposable element-derived 4 OS=Labeo rohita ... [more]
A0A498P4151.161e-5942.80Golgin subfamily A member 6C OS=Labeo rohita OX=84... [more]
A0A498M8V75.891e-5842.80Galectin-3-binding A-like protein OS=Labeo rohita ... [more]
A0A498LZK32.821e-4941.29Ac transposable element-derived 4 OS=Labeo rohita ... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000041838.11.518e-1324.71pep primary_assembly:Astyanax_mexicanus-2.0:14:295... [more]
ENSAMXT00000048878.19.700e-924.78pep primary_assembly:Astyanax_mexicanus-2.0:8:1899... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079481.10.000e+099.61SMESG000079481.1[more]
SMESG000023292.12.853e-18099.22SMESG000023292.1[more]
SMESG000052607.12.163e-17998.44SMESG000052607.1[more]
SMESG000055041.12.286e-166100.00SMESG000055041.1[more]
SMESG000036787.14.590e-14083.20SMESG000036787.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030953 ID=SMED30030953|Name=Dimer_Tnp_hAT domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1177bp
CTCCAAATATGAGTAAACGAAAATGTTTATTTACTGAAGAACTGCAAGCT
AAATTTGTATGTTTTTGCTGATTCATTAATGCATATTTTTCATGAAAAAA
TATTAGAAATAGAATCTGAACAAAATTCTGTATTAGAAGTAATGGCTACA
ACAAATTCATTAGCAAATACATTGCATCATAGATATGAGGAATCATTTTT
TCCAATCAAAGTAAAGTCTATTTTGTCTGAAAATATAAAAAATGGATTGG
AAAATGAAATAGATAAATTCAATTTAAATTGCATGAATTGCTATAAGATG
TCTTATGAATATTTAAAGTTATGGACTAGTCAACTATCCAATTTTGAAAG
CGTTACTTGGATGAAATTAGATAGAGTTCCAACATGGGAAGAAATTGAGA
ATACGATAGTATTGCTTGCATCTAAAAACATCGTTGTTGATGATGTAAAA
TGTTTTGATCAATTTTATAATTTAAAGGTATTTGTAGAAAAAAACATTAA
TATAGAAAATTTTAGAAAATTGATTACCTGTCTCAAATGGACAGAATATT
TTAAAACAAATGCTGTTGAGTTTCATTCAGAACTCTTAAAAATATGTCAA
TTTTTTTTCGCAATACCTGGTCAAAATGCTAATGTTGAAAGAATATTTTC
CATGATTAGCGGCCAGTGGACTAAAGAAAGAAATAAGCTAAAAATTTCCA
CTGTCCGAGGTATTTTGAGCGTAGTTTATAATTATAAAAATATTTCATGT
TTGGAATTTTATAAAGAAATAAAAGAAAATGAAATTTTTTTGAACAAAGT
TAGTAATGTAGATAAATACATTGAAAAAAGTGATCAAAATGATGATGAAG
ATGATTAAATTTAATTTAATTTATATTTTTAAAGTACAAATTTGAATTAT
AAGTGCAATTTTATGTTCCTTAATATCTTTATGTATAAAATTTGATTTTA
ATAATTTTTTCTTTAAAATGTTCTCTTTTTTTGTGTTGACCACTTGACAA
CCCTATCTATAATACATGAAATATTTGCTACCGACAATAATTAATTAATG
TCCTCGATATTTTATTCATATTTTGCTTATAAAATTCGTATGTGCTGCCT
GGCTAAGTCCATTATTAATGACTTTTATATCATATCATTTATTTATCTAT
ATTTTTCTATCATACTGGTAAATTGGT
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protein sequence of SMED30030953-orf-1

>SMED30030953-orf-1 ID=SMED30030953-orf-1|Name=SMED30030953-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=260bp
MHIFHEKILEIESEQNSVLEVMATTNSLANTLHHRYEESFFPIKVKSILS
ENIKNGLENEIDKFNLNCMNCYKMSYEYLKLWTSQLSNFESVTWMKLDRV
PTWEEIENTIVLLASKNIVVDDVKCFDQFYNLKVFVEKNINIENFRKLIT
CLKWTEYFKTNAVEFHSELLKICQFFFAIPGQNANVERIFSMISGQWTKE
RNKLKISTVRGILSVVYNYKNISCLEFYKEIKENEIFLNKVSNVDKYIEK
SDQNDDEDD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0003676nucleic acid binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 95..216