SMED30030947

Overview
NameSMED30030947
Smed IDSMED30030947
Length (bp)1980
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30030947 (SMED30030947) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30030947 (SMED30030947) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030947

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30030947 dd_Smed_v4_86040_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030947 dd_Smed_v4_86040_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30030947 dd_Smed_v4_86040_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30030947 vs. Ensembl Human
Match: FAM200B (family with sequence similarity 200 member B [Source:HGNC Symbol;Acc:HGNC:27740])

HSP 1 Score: 63.929 bits (154), Expect = 6.275e-16
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = -3
Query:  518 EKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITK 754
            ++P C++ + +LA   S++P+KLKR LET H+E   KP+E+FQR   +  +  Q      AV+  ALL+ Y V+YR+ K
Sbjct:   82 DRPQCVICNNILAN-ESLKPSKLKRHLETQHAELIDKPLEYFQRKKKDIKLSTQFLSCSTAVSEKALLSSYLVAYRVAK 159          

HSP 2 Score: 41.2022 bits (95), Expect = 6.275e-16
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = -2
Query:  249 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTS-KFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDIL 491
            M + +F +  A +L+ I   +N+ + RI  I+E +   LI  L++   F I +DE+TDI     L+ Y RY   ++ LED L
Sbjct:  178 MVRTIFDDKSADKLKTIP-NDNTVSLRICTIAEHLETMLITRLQSGIDFAIQLDESTDIGSCTTLLVYVRYAWQDDFLEDFL 258          
BLAST of SMED30030947 vs. Ensembl Human
Match: FAM200B (family with sequence similarity 200 member B [Source:HGNC Symbol;Acc:HGNC:27740])

HSP 1 Score: 63.929 bits (154), Expect = 6.275e-16
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = -3
Query:  518 EKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITK 754
            ++P C++ + +LA   S++P+KLKR LET H+E   KP+E+FQR   +  +  Q      AV+  ALL+ Y V+YR+ K
Sbjct:   82 DRPQCVICNNILAN-ESLKPSKLKRHLETQHAELIDKPLEYFQRKKKDIKLSTQFLSCSTAVSEKALLSSYLVAYRVAK 159          

HSP 2 Score: 41.2022 bits (95), Expect = 6.275e-16
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = -2
Query:  249 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTS-KFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDIL 491
            M + +F +  A +L+ I   +N+ + RI  I+E +   LI  L++   F I +DE+TDI     L+ Y RY   ++ LED L
Sbjct:  178 MVRTIFDDKSADKLKTIP-NDNTVSLRICTIAEHLETMLITRLQSGIDFAIQLDESTDIGSCTTLLVYVRYAWQDDFLEDFL 258          
BLAST of SMED30030947 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 52.7582 bits (125), Expect = 4.693e-15
Identity = 28/84 (33.33%), Postives = 50/84 (59.52%), Query Frame = -2
Query:  231 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSK-FDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 479
            M  ES A ++ Q+ L+N++ A RI E++ D+ +QLI  ++ +K F + +DE  DIA    L+ Y R+   +++ E+  F   +P
Sbjct:  855 MLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVYVRFEHDDDIKEEFFFSASLP 938          

HSP 2 Score: 49.2914 bits (116), Expect = 4.693e-15
Identity = 31/84 (36.90%), Postives = 46/84 (54.76%), Query Frame = -3
Query:  518 IDG-LEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITK 766
            IDG + KP C++   VLA   +M+P+KLKR L + H E + +P EFF+R   E   Q +    +  +   AL A Y V+  + K
Sbjct:  750 IDGEVLKPQCIICGDVLA-NEAMKPSKLKRHLYSKHKEISSQPKEFFERKSSELKSQPKQVFNVSHINISALRASYKVALPVAK 832          
BLAST of SMED30030947 vs. Ensembl Human
Match: ZBED9 (zinc finger BED-type containing 9 [Source:HGNC Symbol;Acc:HGNC:13851])

HSP 1 Score: 52.373 bits (124), Expect = 5.363e-15
Identity = 28/84 (33.33%), Postives = 50/84 (59.52%), Query Frame = -2
Query:  231 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSK-FDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 479
            M  ES A ++ Q+ L+N++ A RI E++ D+ +QLI  ++ +K F + +DE  DIA    L+ Y R+   +++ E+  F   +P
Sbjct:  704 MLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVYVRFEHDDDIKEEFFFSASLP 787          

HSP 2 Score: 49.2914 bits (116), Expect = 5.363e-15
Identity = 31/84 (36.90%), Postives = 46/84 (54.76%), Query Frame = -3
Query:  518 IDG-LEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITK 766
            IDG + KP C++   VLA   +M+P+KLKR L + H E + +P EFF+R   E   Q +    +  +   AL A Y V+  + K
Sbjct:  599 IDGEVLKPQCIICGDVLA-NEAMKPSKLKRHLYSKHKEISSQPKEFFERKSSELKSQPKQVFNVSHINISALRASYKVALPVAK 681          
BLAST of SMED30030947 vs. Ensembl Human
Match: ZBED5 (zinc finger BED-type containing 5 [Source:HGNC Symbol;Acc:HGNC:30803])

HSP 1 Score: 51.2174 bits (121), Expect = 1.413e-14
Identity = 25/83 (30.12%), Postives = 50/83 (60.24%), Query Frame = -2
Query:  234 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLR-TSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 479
            MF E Y+ ++  + L+N++ A RI +++ DI  +L+  L+    F + +DE+ D++  A L+ + RY    ++ ED+L C+ +
Sbjct:  229 MFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVFVRYRFNKSIEEDLLLCESL 311          

HSP 2 Score: 49.2914 bits (116), Expect = 1.413e-14
Identity = 36/93 (38.71%), Postives = 52/93 (55.91%), Query Frame = -3
Query:  482 CLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAV-TPIALLALYHVSYRIT---KYIKIGELLI--CSK 742
            C+L  K+L+  +S+ P+KL+R LET H+ Y  K + FF++ LD     K    K++      A  A Y+VSY I    +   IGELLI  C+K
Sbjct:  132 CVLCKKILS-NSSLAPSKLRRHLETKHAAYKDKDISFFKQHLDSPENNKPPTPKIVNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAK 223          
BLAST of SMED30030947 vs. Ensembl Mouse
Match: Zbed5 (zinc finger, BED type containing 5 [Source:MGI Symbol;Acc:MGI:1919220])

HSP 1 Score: 58.151 bits (139), Expect = 1.018e-15
Identity = 27/94 (28.72%), Postives = 56/94 (59.57%), Query Frame = -2
Query:  213 QIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKF---DIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIPRIASKL 485
            ++M  + +  +L  + L+N++   RI+++S DI +Q++  ++++      I +DE+TD+A    L+ Y RY+   +  ++ L CKP+ R A+ L
Sbjct:  286 RVMIGDEFVTKLSAVSLSNDTVRRRIHDMSADILDQVVQEIKSAPLPICSIQLDESTDVANCLQLMVYVRYINDGDFKDEFLCCKPLERTATAL 379          

HSP 2 Score: 45.4394 bits (106), Expect = 1.018e-15
Identity = 33/109 (30.28%), Postives = 52/109 (47.71%), Query Frame = -3
Query:  509 RQSFQEKAIQFIIFIVRFTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDE-TNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK 832
            R+ + E  +Q+      FTS    G+E+P C++   VL+   SM+P KLKR  E+ HS +  K   +F+   D     +  T  K       A+   Y V+ RI + +K
Sbjct:  166 RRKYNEGFLQY-----GFTSTITVGIERPQCVICGVVLS-AESMKPNKLKRHFESKHSSFAGKDTNYFRSKADGLKKARPDTGSKSNKQNVAAVETSYLVALRIARDMK 268          
BLAST of SMED30030947 vs. UniProt/SwissProt
Match: sp|P0CF97|F200B_HUMAN (Protein FAM200B OS=Homo sapiens OX=9606 GN=FAM200B PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.757e-15
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = -3
Query:  518 EKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITK 754
            ++P C++ + +LA   S++P+KLKR LET H+E   KP+E+FQR   +  +  Q      AV+  ALL+ Y V+YR+ K
Sbjct:   82 DRPQCVICNNILAN-ESLKPSKLKRHLETQHAELIDKPLEYFQRKKKDIKLSTQFLSCSTAVSEKALLSSYLVAYRVAK 159          

HSP 2 Score: 41.2022 bits (95), Expect = 2.757e-15
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = -2
Query:  249 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTS-KFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDIL 491
            M + +F +  A +L+ I   +N+ + RI  I+E +   LI  L++   F I +DE+TDI     L+ Y RY   ++ LED L
Sbjct:  178 MVRTIFDDKSADKLKTIP-NDNTVSLRICTIAEHLETMLITRLQSGIDFAIQLDESTDIGSCTTLLVYVRYAWQDDFLEDFL 258          
BLAST of SMED30030947 vs. UniProt/SwissProt
Match: sp|Q6R2W3|SCND3_HUMAN (SCAN domain-containing protein 3 OS=Homo sapiens OX=9606 GN=ZBED9 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 2.061e-14
Identity = 28/84 (33.33%), Postives = 50/84 (59.52%), Query Frame = -2
Query:  231 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSK-FDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 479
            M  ES A ++ Q+ L+N++ A RI E++ D+ +QLI  ++ +K F + +DE  DIA    L+ Y R+   +++ E+  F   +P
Sbjct:  855 MLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVYVRFEHDDDIKEEFFFSASLP 938          

HSP 2 Score: 49.2914 bits (116), Expect = 2.061e-14
Identity = 31/84 (36.90%), Postives = 46/84 (54.76%), Query Frame = -3
Query:  518 IDG-LEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITK 766
            IDG + KP C++   VLA   +M+P+KLKR L + H E + +P EFF+R   E   Q +    +  +   AL A Y V+  + K
Sbjct:  750 IDGEVLKPQCIICGDVLA-NEAMKPSKLKRHLYSKHKEISSQPKEFFERKSSELKSQPKQVFNVSHINISALRASYKVALPVAK 832          
BLAST of SMED30030947 vs. UniProt/SwissProt
Match: sp|Q49AG3|ZBED5_HUMAN (Zinc finger BED domain-containing protein 5 OS=Homo sapiens OX=9606 GN=ZBED5 PE=2 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 6.206e-14
Identity = 25/83 (30.12%), Postives = 50/83 (60.24%), Query Frame = -2
Query:  234 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLR-TSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 479
            MF E Y+ ++  + L+N++ A RI +++ DI  +L+  L+    F + +DE+ D++  A L+ + RY    ++ ED+L C+ +
Sbjct:  229 MFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVFVRYRFNKSIEEDLLLCESL 311          

HSP 2 Score: 49.2914 bits (116), Expect = 6.206e-14
Identity = 36/93 (38.71%), Postives = 52/93 (55.91%), Query Frame = -3
Query:  482 CLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAV-TPIALLALYHVSYRIT---KYIKIGELLI--CSK 742
            C+L  K+L+  +S+ P+KL+R LET H+ Y  K + FF++ LD     K    K++      A  A Y+VSY I    +   IGELLI  C+K
Sbjct:  132 CVLCKKILS-NSSLAPSKLRRHLETKHAAYKDKDISFFKQHLDSPENNKPPTPKIVNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAK 223          
BLAST of SMED30030947 vs. UniProt/SwissProt
Match: sp|A4Z944|ZBED5_CANLF (Zinc finger BED domain-containing protein 5 OS=Canis lupus familiaris OX=9615 GN=ZBED5 PE=4 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 6.572e-14
Identity = 25/83 (30.12%), Postives = 50/83 (60.24%), Query Frame = -2
Query:  234 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLR-TSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 479
            MF E Y+ ++  + L+N++ A RI +++ DI  +L+  L+    F + +DE+ D++  A L+ + RY    ++ ED+L C+ +
Sbjct:  230 MFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVFVRYRFNKSIEEDLLLCESL 312          

HSP 2 Score: 49.2914 bits (116), Expect = 6.572e-14
Identity = 36/93 (38.71%), Postives = 52/93 (55.91%), Query Frame = -3
Query:  482 CLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAV-TPIALLALYHVSYRIT---KYIKIGELLI--CSK 742
            C+L  K+L+  +S+ P+KL+R LET H+ Y  K + FF++ LD     K    K++      A  A Y+VSY I    +   IGELLI  C+K
Sbjct:  133 CVLCKKILSN-SSLAPSKLRRHLETKHAAYKDKDISFFKQHLDSPENNKPPTPKIVNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAK 224          
BLAST of SMED30030947 vs. UniProt/SwissProt
Match: sp|A4Z943|ZBED5_BOVIN (Zinc finger BED domain-containing protein 5 OS=Bos taurus OX=9913 GN=ZBED5 PE=4 SV=1)

HSP 1 Score: 51.2174 bits (121), Expect = 6.572e-14
Identity = 25/83 (30.12%), Postives = 50/83 (60.24%), Query Frame = -2
Query:  234 MFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLR-TSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 479
            MF E Y+ ++  + L+N++ A RI +++ DI  +L+  L+    F + +DE+ D++  A L+ + RY    ++ ED+L C+ +
Sbjct:  230 MFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVFVRYRFNKSIEEDLLLCESL 312          

HSP 2 Score: 48.9062 bits (115), Expect = 6.572e-14
Identity = 36/93 (38.71%), Postives = 52/93 (55.91%), Query Frame = -3
Query:  482 CLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAV-TPIALLALYHVSYRIT---KYIKIGELLI--CSK 742
            C+L  K+L+  +S+ P+KL+R LET H+ Y  K + FF++ LD     K    K++      A  A Y+VSY I    +   IGELLI  C+K
Sbjct:  133 CVLCKKILSN-SSLAPSKLRRHLETKHAAYKDKDISFFKQHLDSPENNKPPTPKIVNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAK 224          
BLAST of SMED30030947 vs. TrEMBL
Match: A0A2S2R4C3 (SCAN domain-containing protein 3 OS=Sipha flava OX=143950 GN=SCAND3_6 PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.554e-29
Identity = 39/86 (45.35%), Postives = 60/86 (69.77%), Query Frame = -2
Query:  234 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 491
            M  IM  ES    L ++ L+NN+ + RI+ ++ED+ +QLI  ++  +F + +DEATD  KDAHLI Y R++ G++M+ED+LFCK I
Sbjct:  152 MVNIMVGESAGKLLSKVPLSNNTVSRRIHHMAEDLNDQLIEKIKEKEFGLQLDEATDNNKDAHLICYVRFIDGDDMVEDLLFCKNI 237          

HSP 2 Score: 76.2554 bits (186), Expect = 3.554e-29
Identity = 48/100 (48.00%), Postives = 59/100 (59.00%), Query Frame = -3
Query:  491 FTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 781
            FTS  I+G E+P+C+L  KVLAP   M P+KLKR LE  H     KP ++F R L E   +K TF K  ++   AL+A Y VSYRI K  K   I E LI
Sbjct:   47 FTSTEINGEERPLCVLCMKVLAP-ECMLPSKLKRHLEANHKYTIGKPRDYFIRKLKELKQEKSTFFKNASIPTNALIASYKVSYRIAKCKKPHTIAEELI 145          
BLAST of SMED30030947 vs. TrEMBL
Match: A0A1B6ISX9 (Uncharacterized protein OS=Homalodisca liturata OX=320908 GN=g.41333 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 7.380e-29
Identity = 47/89 (52.81%), Postives = 60/89 (67.42%), Query Frame = -2
Query:  234 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 500
            A  M +IM  E    QL +I L+NN+ + RI++ISEDI  QLI  LR  +F I +DEATD  KDA+LI Y RYV  N ++ED+LFCK I
Sbjct:  154 AVDMAKIMIGEDAGKQLLKIPLSNNTISRRIDDISEDINKQLINSLRGKEFGIQLDEATDSHKDANLICYVRYVNENQLIEDLLFCKEI 242          

HSP 2 Score: 69.3218 bits (168), Expect = 7.380e-29
Identity = 44/100 (44.00%), Postives = 56/100 (56.00%), Query Frame = -3
Query:  491 FTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 781
            F+ + I+  E+P C++  KVL+   SM P+KLKR LET+HS    KP EFF R   E   QK +F K       ALLA + V+Y I K  K   I E LI
Sbjct:   52 FSCIEINNEERPQCVVCMKVLS-VESMLPSKLKRHLETVHSNLIGKPREFFIRKKTEAVKQKSSFSKHSKANSDALLASFKVAYHIAKCKKPHTIAEELI 150          
BLAST of SMED30030947 vs. TrEMBL
Match: A0A498NJT7 (Zinc finger BED domain-containing 5-like protein OS=Labeo rohita OX=84645 GN=ROHU_004686 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 2.324e-27
Identity = 39/91 (42.86%), Postives = 61/91 (67.03%), Query Frame = -2
Query:  219 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIPRIAS 491
            M  +M  E+   +L+ I ++N++ + RI +I+ DI  QLI  +R ++F + VDEATD  KD+ LIAY R+V   ++ ED+LFCK IP+ A+
Sbjct:  146 MVSVMLDEASVAKLKTIPVSNDTISRRIQDIANDIEEQLIEKIRGTRFALQVDEATDSNKDSLLIAYVRFVDFESLNEDLLFCKYIPKRAT 236          

HSP 2 Score: 76.2554 bits (186), Expect = 2.324e-27
Identity = 43/99 (43.43%), Postives = 62/99 (62.63%), Query Frame = -3
Query:  491 SVNIDGLE-KPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 775
            +VN+ G E +PMC+L  K LA  +SM+P KL+R +E++H  Y +KP+EFF+R LD+   Q+  F K    T  A LA Y ++YR+ +  K   I E LI
Sbjct:   42 TVNVVGEEERPMCVLCLKTLAA-DSMKPTKLRRHIESMHPNYVQKPIEFFKRKLDQYRGQQSRFVKATTATAKAQLASYKIAYRVAQCKKSHTIAEELI 139          
BLAST of SMED30030947 vs. TrEMBL
Match: A0A498MLE5 (Zinc finger BED domain-containing 5-like protein OS=Labeo rohita OX=84645 GN=ROHU_025652 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.842e-27
Identity = 43/99 (43.43%), Postives = 62/99 (62.63%), Query Frame = -3
Query:  491 SVNIDGLE-KPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 775
            +VN+ G E +PMC+L  K LA  +SM+P KL+R +E++H  Y +KP+EFF+R LD+   Q+  F K    T  A LA Y ++YR+ +  K   I E LI
Sbjct:   42 TVNVVGEEERPMCVLCLKTLAA-DSMKPTKLRRHIESMHPNYVQKPIEFFKRKLDQYRGQQSRFVKATTATAKAQLASYKIAYRVAQCKKSHTIAEELI 139          

HSP 2 Score: 76.2554 bits (186), Expect = 3.842e-27
Identity = 39/91 (42.86%), Postives = 60/91 (65.93%), Query Frame = -2
Query:  219 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIPRIAS 491
            M  +M  E+   +L+ I ++N++ + RI +I+ DI  QLI  +R  +F + VDEATD  KD+ LIAY R+V   ++ ED+LFCK IP+ A+
Sbjct:  146 MVSVMLDEASVAKLKTIPVSNDTISRRIQDIANDIEEQLIEKIRGKRFALQVDEATDSNKDSLLIAYVRFVDFESLNEDLLFCKYIPKRAT 236          
BLAST of SMED30030947 vs. TrEMBL
Match: A0A087UJ93 (SCAN domain-containing protein 3 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_09699 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.369e-26
Identity = 50/117 (42.74%), Postives = 71/117 (60.68%), Query Frame = -3
Query:  491 RQSFQEKAIQFIIFIVRFTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 832
            ++ + E  +QF      FT  N +G E+P+CL+ +K+LA   SM+P+KLKR LE+ H  Y  KP E+F+R+    N +K+ F+KL+ V    LLA Y VSY I K  K   IGE L+
Sbjct:   37 KRKYDESFLQF-----GFTLQNCEGNEQPLCLICNKLLAS-ESMKPSKLKRHLESKHISYVNKPKEYFERLYKSLNKEKKIFEKLITVNEKYLLASYEVSYCIAKNKKPFTIGEDLV 147          

HSP 2 Score: 66.6254 bits (161), Expect = 1.369e-26
Identity = 34/90 (37.78%), Postives = 58/90 (64.44%), Query Frame = -2
Query:  234 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTS-KFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI 500
            A  M +I+    Y   +++I L+N++ ++RI++I++D   QLI  ++ S KF I +DE TD  K A L+ Y RYV   ++ E++LFC+P+
Sbjct:  151 AIKMVEILHGRKYGDDIRKIPLSNDTVSNRISDINKDQLIQLITRIKESPKFSIQLDETTDFTKLAQLLVYVRYVYKESVEEELLFCRPM 240          
BLAST of SMED30030947 vs. Ensembl Cavefish
Match: ENSAMXT00000051422.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:35461191:35465166:-1 gene:ENSAMXG00000032672.1 transcript:ENSAMXT00000051422.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.1814 bits (191), Expect = 7.290e-27
Identity = 40/91 (43.96%), Postives = 57/91 (62.64%), Query Frame = -2
Query:  231 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKF-DIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 500
            A  M  +M  E    QL  I L++N+   RINE++ D+  QLI  +R S+F  I +DE+TDIA  ++L+A+ RY     + EDILFCKP+P
Sbjct:  198 AKEMVSVMLGEKPCKQLNLISLSDNTVERRINEMAHDVTQQLIKRVRESRFYAIQLDESTDIANLSNLLAFVRYEHNREIFEDILFCKPLP 288          

HSP 2 Score: 63.1586 bits (152), Expect = 7.290e-27
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = -3
Query:  491 FTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK-------IGELLI 781
            FT    +    P+C++ S+VLA   +++P KL+R LET HSEY  KP+EFF+  L E   +K+T +  LA +          SYR+ + I        IGE LI
Sbjct:   94 FTCTGTENEPLPLCVVCSEVLA-NEALKPTKLRRHLETKHSEYASKPVEFFENKLKEYQSRKKTLE--LACSGTDNAKAVEASYRVAQLIAKAGKPHTIGESLI 194          
BLAST of SMED30030947 vs. Ensembl Cavefish
Match: ENSAMXT00000044261.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000070.1:2279767:2281653:1 gene:ENSAMXG00000037161.1 transcript:ENSAMXT00000044261.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.1814 bits (191), Expect = 7.500e-26
Identity = 40/91 (43.96%), Postives = 57/91 (62.64%), Query Frame = -2
Query:  231 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKF-DIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 500
            A  M  +M  E    QL  I L++N+   RINE++ D+  QLI  +R S+F  I +DE+TDIA  ++L+A+ RY     + EDILFCKP+P
Sbjct:  155 AKEMVSVMLGEKPCKQLNLISLSDNTVERRINEMAHDVTQQLIKRVRESRFYAIQLDESTDIANLSNLLAFVRYEHNREIFEDILFCKPLP 245          

HSP 2 Score: 59.3066 bits (142), Expect = 7.500e-26
Identity = 38/104 (36.54%), Postives = 55/104 (52.88%), Query Frame = -3
Query:  491 FTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK-------IGELLI 781
            FT    +    P+C++ S+VLA   +++  KL+R LET HSEY  KP+EFF+  L E   +K+T +  LA +          SYR+ + I        IGE LI
Sbjct:   51 FTCTGTENEPLPLCVVCSEVLA-NEALKTTKLRRHLETKHSEYASKPVEFFENKLKEYQSRKKTLE--LACSGTDHAKAVEASYRVAQLIAKAGKPHTIGESLI 151          
BLAST of SMED30030947 vs. Ensembl Cavefish
Match: ENSAMXT00000057314.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:4224188:4227217:-1 gene:ENSAMXG00000029268.1 transcript:ENSAMXT00000057314.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.5362 bits (140), Expect = 1.370e-8
Identity = 29/81 (35.80%), Postives = 50/81 (61.73%), Query Frame = -2
Query:  249 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDIL 491
            M + M     A + + + L+NN+   RI+ ++ ++ N L+  L+TS F I +DE T +A++A LI YA+Y+ G  + +DIL
Sbjct:  125 MAERMCGPQVANKFKIVPLSNNTVKDRIDRMAGNVENTLVEKLKTSPFSIQLDETTTVAEEAILIVYAQYIEGTEVKQDIL 205          
BLAST of SMED30030947 vs. Ensembl Medaka
Match: ENSORLT00000040389.1 (pep primary_assembly:ASM223467v1:19:265767:268275:-1 gene:ENSORLG00000025045.1 transcript:ENSORLT00000040389.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.0182 bits (175), Expect = 2.095e-27
Identity = 44/100 (44.00%), Postives = 58/100 (58.00%), Query Frame = -3
Query:  491 FTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 781
            FT   +   E+P+CLL  K+LA  +SM+P KL+R L+TLH  +  KP+EFFQR   E   Q   F    +V   ALLA Y V+Y+I +  K   I E LI
Sbjct:   41 FTVTTVGDEERPVCLLCLKMLAA-DSMKPNKLRRHLKTLHPNHADKPLEFFQRKRAEYCQQSSRFVSATSVNQRALLASYKVAYQIAQCKKPHTIAEELI 139          

HSP 2 Score: 70.8626 bits (172), Expect = 2.095e-27
Identity = 37/101 (36.63%), Postives = 61/101 (60.40%), Query Frame = -2
Query:  195 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI--PRIASKLFPQSTC 491
            M  +M  ++ A +L+ + L+N++ A RIN+I+ D+  QL+  L+  +F +  DEATD  KD   IAY R+ + +++ ED+LFCK +     A +LF    C
Sbjct:  146 MVSVMLDDASAAKLKTVPLSNDTVARRINDIANDLKEQLVEKLKDKRFALQFDEATDSNKDCLFIAYVRFDMTHSLCEDLLFCKYVRDRATAEELFKMLDC 246          
BLAST of SMED30030947 vs. Ensembl Medaka
Match: ENSORLT00000035512.1 (pep primary_assembly:ASM223467v1:11:22712371:22714941:1 gene:ENSORLG00000026495.1 transcript:ENSORLT00000035512.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 72.0182 bits (175), Expect = 2.238e-27
Identity = 44/100 (44.00%), Postives = 58/100 (58.00%), Query Frame = -3
Query:  491 FTSVNIDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 781
            FT   +   E+P+CLL  K+LA  +SM+P KL+R L+TLH  +  KP+EFFQR   E   Q   F    +V   ALLA Y V+Y+I +  K   I E LI
Sbjct:   41 FTVTTVGDEERPVCLLCLKMLAA-DSMKPNKLRRHLKTLHPNHADKPLEFFQRKRAEYCQQSSRFVSATSVNQRALLASYKVAYQIAQCKKPHTIAEELI 139          

HSP 2 Score: 70.8626 bits (172), Expect = 2.238e-27
Identity = 37/101 (36.63%), Postives = 61/101 (60.40%), Query Frame = -2
Query:  195 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPI--PRIASKLFPQSTC 491
            M  +M  ++ A +L+ + L+N++ A RIN+I+ D+  QL+  L+  +F +  DEATD  KD   IAY R+ + +++ ED+LFCK +     A +LF    C
Sbjct:  146 MVSVMLDDASAAKLKTVPLSNDTVARRINDIANDLKEQLVEKLKDKRFALQFDEATDSNKDCLFIAYVRFDMTHSLCEDLLFCKYVRDRATAEELFKMLDC 246          
BLAST of SMED30030947 vs. Ensembl Medaka
Match: ENSORLT00000035028.1 (pep primary_assembly:ASM223467v1:2:7372701:7381259:1 gene:ENSORLG00000027324.1 transcript:ENSORLT00000035028.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.003 bits (149), Expect = 7.411e-18
Identity = 28/91 (30.77%), Postives = 54/91 (59.34%), Query Frame = -2
Query:  231 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKF-DIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 500
            A  M  I F      ++ +I L+N++   RI  ++E + +QL+  LR S+F  + +DE+TD+  +A+L+ + RY+    + ++ LFC+ +P
Sbjct:   94 AKVMANIFFGGKAGDEINRIPLSNDTVQRRIKAMAESVEHQLVTHLRQSQFFSLQLDESTDLGNEANLLCFVRYIYAGGLHDEFLFCRSLP 184          

HSP 2 Score: 48.521 bits (114), Expect = 7.411e-18
Identity = 34/78 (43.59%), Postives = 44/78 (56.41%), Query Frame = -3
Query:  491 SMRPAKLKRSLETLHSEYTKKPMEFFQRMLDE--TNIQKQTFKKLLAVTPI-ALLALYHVSYRITKYIK---IGELLI 706
            +M+PAKLKR LET H EY  KP+ FF+R  DE   ++ K+T +       + A  A Y VS  I K  K   IGE L+
Sbjct:   13 AMKPAKLKRHLETKHKEYQGKPIGFFERKCDELKKHMMKETSQFFAPGENVKATEASYKVSLLIAKAGKPHSIGENLV 90          
BLAST of SMED30030947 vs. Ensembl Medaka
Match: ENSORLT00000032459.1 (pep primary_assembly:ASM223467v1:8:14240678:14247355:1 gene:ENSORLG00000024991.1 transcript:ENSORLT00000032459.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.2326 bits (147), Expect = 5.360e-17
Identity = 35/95 (36.84%), Postives = 57/95 (60.00%), Query Frame = -2
Query:  219 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKF-DIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIPRIAS 500
            A  M + M  E+ A +L+ I L+N+  + RI +I+ DI  QL+  +R S F  + +DE+TD+   A L+ + RY   +++ EDILFC  +P  A+
Sbjct:  126 AVDMCREMIGEAAAKKLKTIPLSNDIVSHRIADIASDIEQQLLERIRKSPFFSLQLDESTDVTNAALLLVFVRYRWDSSLQEDILFCGELPTRAT 220          

HSP 2 Score: 46.595 bits (109), Expect = 5.360e-17
Identity = 32/104 (30.77%), Postives = 51/104 (49.04%), Query Frame = -3
Query:  491 IVRFTSVNIDGLEKPMCLLYSKVLAPYN-SMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRIT---KYIKIGELLI 790
            ++ F  VN     +P        L   N +++P+KLKR LET H +   KP+++F+R  D   +Q+++   L      AL A Y V+ R+    K   I E L+
Sbjct:   19 LINFGFVNGGSEAEPRAQCVECGLTLSNEALKPSKLKRHLETKHPKLIGKPVDYFKRKDDGLQMQRRSIVSLTGDAKCALKASYLVARRVAQSKKAFTIAEELV 122          
BLAST of SMED30030947 vs. Ensembl Medaka
Match: ENSORLT00000028620.1 (pep primary_assembly:ASM223467v1:3:35179252:35181570:1 gene:ENSORLG00000028714.1 transcript:ENSORLT00000028620.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.077 bits (144), Expect = 8.326e-16
Identity = 34/95 (35.79%), Postives = 57/95 (60.00%), Query Frame = -2
Query:  219 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKF-DIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIPRIAS 500
            A  M + M  E+ A +L+ I L+N+  + RI +++ DI  QL+  +R S F  + +DE+TD+   A L+ + RY   +++ EDILFC  +P  A+
Sbjct:  172 AVDMCREMIGEAAAKKLKTIPLSNDIVSHRIADMASDIEQQLLGRIRKSPFFSLQLDESTDVTNAALLLVFVRYRWDSSLQEDILFCGELPTRAT 266          

HSP 2 Score: 43.8986 bits (102), Expect = 8.326e-16
Identity = 31/103 (30.10%), Postives = 51/103 (49.51%), Query Frame = -3
Query:  491 VRFTSVNIDGLEKPMCLLYSKVLAPYN-SMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRIT---KYIKIGELLI 787
            ++F  VN     +P        L   N +++P+KLKR LET + +   KP+++F+R  D   +Q+++   L      AL A Y V+ R+    K   I E L+
Sbjct:   66 IKFGFVNGGSEAEPRAQCVECGLTLSNEALKPSKLKRHLETKYPKLIGKPVDYFKRKDDGLQMQRRSIVSLTGDAKCALKASYLVARRVALNKKAFTIAEELV 168          
BLAST of SMED30030947 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.279e-20
Identity = 48/93 (51.61%), Postives = 64/93 (68.82%), Query Frame = 1
Query:   13 ENIHL-FCGSLGDTF-REKYICAKIDHHSRYISLTPISRQDENTIKKVLLNNWILKFGPPNQVHVDCGKSFEAILGIGLQNNISSNILFPTTY 285
            E I++  CG L +TF ++KYIC  IDH+S+YISLT I++QDE TI + LLN WILKFG P ++HVDCGK+FEA     L       ++F + Y
Sbjct: 3294 EKIYMDICGPLKETFNKKKYICGIIDHYSKYISLTAINKQDERTISETLLNKWILKFGAPKELHVDCGKNFEARSIKELAKTAGIELIFSSPY 3386          
BLAST of SMED30030947 vs. Planmine SMEST
Match: SMESG000015406.1 (SMESG000015406.1)

HSP 1 Score: 63.929 bits (154), Expect = 1.674e-19
Identity = 31/90 (34.44%), Postives = 57/90 (63.33%), Query Frame = -2
Query:  231 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIP 500
            A  + + +F E  A ++  + L+N++ A +I +++ +I ++LI+ L+ S F + +DE+TDIA  A L+ + RY   N   ED+L C+P+P
Sbjct:  219 AVDIAKCIFDEKSAKEITDVPLSNDTVARQIKDLASNIKSKLIFRLKNSNFSLQMDESTDIAGLAILLVFVRYQHTNLFEEDLLLCEPLP 308          

HSP 2 Score: 51.9878 bits (123), Expect = 1.674e-19
Identity = 37/95 (38.95%), Postives = 49/95 (51.58%), Query Frame = -3
Query:  491 IDGLEKPMCLLYSKVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRIT---KYIKIGELLI 766
            ID    P C+L SK+  P ++M PAK++R LET+H E+  K ++FF    DE N +             A  A Y VSYRI    +   IGE LI
Sbjct:  135 IDANNVPQCVLCSKMF-PNSAMVPAKMRRHLETIHPEFKHKQVDFFILKSDEINEK-------------ATEASYIVSYRIALAGEAHTIGETLI 215          
BLAST of SMED30030947 vs. Planmine SMEST
Match: SMESG000059630.1 (SMESG000059630.1)

HSP 1 Score: 71.2478 bits (173), Expect = 7.905e-13
Identity = 34/67 (50.75%), Postives = 47/67 (70.15%), Query Frame = -2
Query:  291 MFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAY 491
            M ++M  ESYA QL+ I LA+N+   RI++ISED+C+ LI  ++ S F +  DEA D+ KDAHLI Y
Sbjct:  119 MIEMMLGESYADQLKIIPLADNTVGRRISDISEDLCDLLIEKVKLSSFALQADEAIDVLKDAHLITY 185          
BLAST of SMED30030947 vs. Planmine SMEST
Match: SMESG000019238.1 (SMESG000019238.1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.402e-12
Identity = 33/84 (39.29%), Postives = 53/84 (63.10%), Query Frame = -2
Query:  219 ESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAHLIAYARYVVGNNMLEDILFCKPIPRIAS 470
            E++A +L+ I L+N++ A R+  ++ED+ +QL   L    F I +DE  D  KDAHLIAY  +  G + +E++LF K I + A+
Sbjct:   47 ENFAKKLKSIPLSNDTVAPRVGGMAEDVQHQLFARLCEKLFSIQLDETIDNNKDAHLIAYVHFCDGVSAVEELLFWKSIQQRAT 130          

HSP 2 Score: 30.4166 bits (67), Expect = 1.402e-12
Identity = 20/41 (48.78%), Postives = 25/41 (60.98%), Query Frame = -3
Query:  491 IQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 604
            +QK  FK  L+V   ALLA Y V Y+I++  K   IGE LI
Sbjct:    1 MQKLVFKNTLSVNEQALLASYKVLYQISRCKKPHTIGEELI 41          
BLAST of SMED30030947 vs. Planmine SMEST
Match: SMESG000073961.1 (SMESG000073961.1)

HSP 1 Score: 50.447 bits (119), Expect = 1.426e-12
Identity = 26/66 (39.39%), Postives = 41/66 (62.12%), Query Frame = -2
Query:  303 AAYMFQIMFSESYA*QLQQILLANNSFASRINEISEDICNQLIYWLRTSKFDIGVDEATDIAKDAH 500
            A  + + MF E++A +L+ I L+N++ A  +  ++ED+ +QL   LR   F I +DEA D  KDAH
Sbjct:  269 AIEIVETMFGENFAKKLKSIPLSNDTVARHVGGMAEDVQHQLFAKLRRKLFSIQLDEAIDNNKDAH 334          

HSP 2 Score: 41.9726 bits (97), Expect = 1.426e-12
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = -3
Query:  491 EYTKKPMEFFQRMLDETNIQKQTFKKLLAVTPIALLALYHVSYRITKYIK---IGELLI 658
            EY  K  +FF+  L     QK  FK  L+V   ALLA Y VSY+I +  K   IGE LI
Sbjct:  207 EYVNKSRQFFESKLKSYEKQKLVFKNTLSVNEKALLASYKVSYQIARCKKPHTIGEELI 265          
The following BLAST results are available for this feature:
BLAST of SMED30030947 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FAM200B6.275e-1640.51family with sequence similarity 200 member B [Sour... [more]
FAM200B6.275e-1640.51family with sequence similarity 200 member B [Sour... [more]
ZBED94.693e-1533.33zinc finger BED-type containing 9 [Source:HGNC Sym... [more]
ZBED95.363e-1533.33zinc finger BED-type containing 9 [Source:HGNC Sym... [more]
ZBED51.413e-1430.12zinc finger BED-type containing 5 [Source:HGNC Sym... [more]
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BLAST of SMED30030947 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Zbed51.018e-1528.72zinc finger, BED type containing 5 [Source:MGI Sym... [more]
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BLAST of SMED30030947 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0CF97|F200B_HUMAN2.757e-1540.51Protein FAM200B OS=Homo sapiens OX=9606 GN=FAM200B... [more]
sp|Q6R2W3|SCND3_HUMAN2.061e-1433.33SCAN domain-containing protein 3 OS=Homo sapiens O... [more]
sp|Q49AG3|ZBED5_HUMAN6.206e-1430.12Zinc finger BED domain-containing protein 5 OS=Hom... [more]
sp|A4Z944|ZBED5_CANLF6.572e-1430.12Zinc finger BED domain-containing protein 5 OS=Can... [more]
sp|A4Z943|ZBED5_BOVIN6.572e-1430.12Zinc finger BED domain-containing protein 5 OS=Bos... [more]
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BLAST of SMED30030947 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2S2R4C33.554e-2945.35SCAN domain-containing protein 3 OS=Sipha flava OX... [more]
A0A1B6ISX97.380e-2952.81Uncharacterized protein OS=Homalodisca liturata OX... [more]
A0A498NJT72.324e-2742.86Zinc finger BED domain-containing 5-like protein O... [more]
A0A498MLE53.842e-2743.43Zinc finger BED domain-containing 5-like protein O... [more]
A0A087UJ931.369e-2642.74SCAN domain-containing protein 3 (Fragment) OS=Ste... [more]
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BLAST of SMED30030947 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSAMXT00000051422.17.290e-2743.96pep primary_assembly:Astyanax_mexicanus-2.0:15:354... [more]
ENSAMXT00000044261.17.500e-2643.96pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000057314.11.370e-835.80pep primary_assembly:Astyanax_mexicanus-2.0:1:4224... [more]
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BLAST of SMED30030947 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030947 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000040389.12.095e-2744.00pep primary_assembly:ASM223467v1:19:265767:268275:... [more]
ENSORLT00000035512.12.238e-2744.00pep primary_assembly:ASM223467v1:11:22712371:22714... [more]
ENSORLT00000035028.17.411e-1830.77pep primary_assembly:ASM223467v1:2:7372701:7381259... [more]
ENSORLT00000032459.15.360e-1736.84pep primary_assembly:ASM223467v1:8:14240678:142473... [more]
ENSORLT00000028620.18.326e-1635.79pep primary_assembly:ASM223467v1:3:35179252:351815... [more]
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BLAST of SMED30030947 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029259.12.279e-2051.61SMESG000029259.1[more]
SMESG000015406.11.674e-1934.44SMESG000015406.1[more]
SMESG000059630.17.905e-1350.75SMESG000059630.1[more]
SMESG000019238.11.402e-1239.29SMESG000019238.1[more]
SMESG000073961.11.426e-1239.39SMESG000073961.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030947 ID=SMED30030947|Name=SMED30030947|organism=Schmidtea mediterranea sexual|type=transcript|length=1980bp
AGAACCGATGGAGAAAATATACATTTATTTTGCGGTTCATTAGGAGACAC
GTTTAGAGAAAAATATATTTGTGCAAAAATCGATCATCACAGTAGATACA
TTTCATTAACTCCTATTAGTAGGCAGGATGAAAACACCATAAAGAAAGTT
TTATTAAATAATTGGATATTGAAGTTTGGACCACCTAATCAGGTACATGT
TGATTGTGGAAAGAGTTTCGAAGCAATTCTAGGAATAGGTTTACAAAATA
ATATATCTTCTAACATATTATTTCCCACAACATATCGAGCGTATGCAATT
AAATGGGCATCTTTAGCTATATCAGTTGCCTCATCTACCCCTATATCGAA
TTTCGAAGTACGTAGCCAATAAATTAATTGATTGCAAATATCTTCAGATA
TTTCATTGATTCTACTTGCAAATGAGTTATTAGCAAGTAGAATTTGTTGT
AACTGTTATGCATACGATTCGCTGAACATTATTTGGAACATATAAGCAGC
TCTCCTATTTTTATATATTTTGTGATTCGATAAGAGACATGGTACAAAGC
CAATAAGGCAATTGGTGTAACTGCGAGTAATTTTTTAAACGTTTGCTTCT
GAATATTTGTTTCGTCCAACATTCTTTGAAAAAATTCCATGGGCTTTTTA
GTGTATTCAGAATGTAGAGTTTCTAGGCTCCGTTTTAACTTGGCTGGTCT
CATACTATTATATGGAGCCAAAACTTTCGAATACAACAAACACATGGGTT
TTTCCAAGCCATCTATATTAACGCTTGTGAATCTAACGATAAATATGATA
AACTGTATCGCCTTTTCTTGGAAGCTTTGTCTCAGTTTTGCATTAATTAC
CACTTCTATAAGCATCAGTATCTTCATTATTATCGATTTTTGTTTTGTAA
CCACTAAAGTTTATCCATTCGGTTAAAAATATTTTATTGATGCAATTTAA
AATAAATAATATACTTTTAAAATACTAACGATTATTTTCAGAAATCATGA
TAAAAACAATTTATACACCCCCACAAAATGCTTCAATTGACACATTAACT
ACGAGTGACGTCTAAATATGAAGTTATTGTTTCATGCACCTAGAGATCGT
TCTGTGCATTAGAAACAGAGCGCCATTTTTAAAATCTCTCCGGAGTGTAT
AAAAATGAAGTTTAAAGAAAAAAAGAAGGATTCATCTATAGCATTTTTAT
TGTTAAGTTACGATATGATGTGAAAATAATTTTTCGTTAATATGTTTTGA
TGCCTTCCCTAGCAATCGTTAGCGCCTCCCAGTTTGGAAACAGCTGATTT
ACATGACAAATATTAAATGCTAAATTAATTAATATATTTTTTTAAAATTA
ATATGAGACATTTATGATTGTAAGACTATAAATCAAAAAATTTATTTTGG
TTTAGCATTACAATTATAAATATGAAAGTTATTGAATAAAATAAAAGGTC
AAAGGTTAAATCAAAATTTGATGCTAAAATTAGACAAGTAAATAAATAAC
ACGATAATAATTTGCTGCAAAGTATTAAATTAAATGATTTCATAAATTAC
TTGTAGTAAAGGTTTGTTTAATTTTCATCCATTCGGAAATAAAATTGTAA
CCTTGTTGTGTTATAATTTTCATCAAATAATGTGTTAAATCTGTCCCAGC
CAATTTCTTTGCATTTAACGCATCATTGAATTTATCAGTAGTCATCATCT
TAACACAACATCAAGCGCAATGGAATTATCAACTACAACTTCTTGTTCGT
GGAACATTAAATCTTTTTTTAAAGATGTATTAATACATACATATGTTAAC
ATATAAGTACTTATTTGTCCAAACCAGTATTGACCAATGAATGCACAACC
ATAAATATATACTCTGTGTAGAGAAAACGATAAGAAAGGGTTACATAAAA
CTAGAGTTTGGAATTTCCCTCAAAATTTTATTGTGCGAAGTCGGAAATTT
TTTTTCTCAAGAATATAGAGAATTCCTGAG
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protein sequence of SMED30030947-orf-1

>SMED30030947-orf-1 ID=SMED30030947-orf-1|Name=SMED30030947-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp
RTDGENIHLFCGSLGDTFREKYICAKIDHHSRYISLTPISRQDENTIKKV
LLNNWILKFGPPNQVHVDCGKSFEAILGIGLQNNISSNILFPTTYRAYAI
KWASLAISVASSTPISNFEVRSQ*
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protein sequence of SMED30030947-orf-2

>SMED30030947-orf-2 ID=SMED30030947-orf-2|Name=SMED30030947-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=133bp
MKILMLIEVVINAKLRQSFQEKAIQFIIFIVRFTSVNIDGLEKPMCLLYS
KVLAPYNSMRPAKLKRSLETLHSEYTKKPMEFFQRMLDETNIQKQTFKKL
LAVTPIALLALYHVSYRITKYIKIGELLICSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR036397RNaseH_sf
IPR012337RNaseH-like_sf
IPR001584Integrase_cat-core
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
Vocabulary: biological process
TermDefinition
GO:0015074DNA integration
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 1..83
e-value: 1.4E-6
score: 29.9
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 5..80