GCR115

Overview
NameGCR115
Smed IDSMED30030936
Length (bp)1601
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR115 (SMED30030936) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR115 (SMED30030936) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR115 (SMED30030936) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR115 (SMED30030936) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030936

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30030936h1SMcG0009663 SmedASXL_008253SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30030936h1SMcG0009663 dd_Smed_v4_8144_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030936h1SMcG0009663 dd_Smed_v4_8144_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30030936h1SMcG0009663 dd_Smed_v4_8144_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of GCR115 vs. TrEMBL
Match: A0A193KUN2 (GCR115 OS=Schmidtea mediterranea OX=79327 GN=gcr115 PE=2 SV=1)

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 2 
Query:    2 YFLPVLCMDEICWPWTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTSNHEDSFLVYALDXXXXXXXXXXXXXXXXXXXXXPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKXXXXXXXXXXXXETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH 1477
            YFLPVLCMDEICWP+TKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTSNHEDSFLVYALDIAATSLSLVASILLISAYLIIPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHISETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH
Sbjct:   40 YFLPVLCMDEICWPFTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTSNHEDSFLVYALDIAATSLSLVASILLISAYLIIPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHISETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH 531
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BLAST of GCR115 vs. Planmine SMEST
Match: SMESG000051770.1 (SMESG000051770.1)

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 491/492 (99.80%), Postives = 492/492 (100.00%), Query Frame = 2 
Query:    2 YFLPVLCMDEICWPWTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTSNHEDSFLVYALDXXXXXXXXXXXXXXXXXXXXXPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKXXXXXXXXXXXXETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH 1477
            YFLPVLCMDEICWP+TKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTSNHEDSFLVYALDIAATSLSLVASILLISAYLIIPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHISETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH
Sbjct:   40 YFLPVLCMDEICWPFTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTSNHEDSFLVYALDIAATSLSLVASILLISAYLIIPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHISETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH 531
[Close]
BLAST of GCR115 vs. Planmine SMEST
Match: SMESG000051772.1 (SMESG000051772.1)

HSP 1 Score: 327.405 bits (838), Expect = 1.424e-105
Identity = 202/490 (41.22%), Postives = 285/490 (58.16%), Query Frame = 2 
Query:   26 DEICWPWTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQFS------CMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVV---KNSSLKNTTSNHEDSFLVYALDXXXXXXXXXXXXXXXXXXXXXPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKXXXXXXXXXXXXETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKA 1468
              ICWP T   +L MIS C     +  L  KCE+PN+FL  IVDY P + +    +VLYRN YC +C+ I +Y+++  N   +C   +   K K + +Y A ML+   C        Y+ F       C +P   C      N ++F GY   N  ++WF F    CC N S IC+  S  R+ L  Y++N ++Y +  E V    NSSL  +    E+S L Y   +    LSL ++ LLI  Y  I +LQTSTSYMI+ IG NIA +E+  +L  F C  C+   C++    SI+FHISE ICIS Y+ SFW+DL+QMR  + N     RRN KR V F+  S +IP+      L+F + DF       + ++C  LK+ PL  FYI+P+ ++L++TT++ +KIC YL SS+ I+ +E   ++ Q F +GLKLCFISGITWIFN +M+  PN I+ D G+++SHFNGFVIVVCFLS + VRK I +++
Sbjct:   75 QPICWPMTSVSYLFMISNCSDINTDPILRRKCEDPNLFLPYIVDYIP-LHRRGSKSVLYRNRYCIRCNKIKEYEYVNTNNTFRCSKKIVLGKGKQKENYLALMLFKRLC--------YANFHFPESLRCSLPEEKCKIPETVNEDTFIGYGIDNSILRWFRFQGSECCLNYSEICMPISRRRTPLLIYALNEMIYPDDFETVNNNTNSSLNISIYRQEESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKLCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMR--TPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQVCSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYLRSSIPIN-HERHCKSHQRFFIGLKLCFISGITWIFNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQS 552
The following BLAST results are available for this feature:
BLAST of GCR115 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A193KUN20.000e+099.80GCR115 OS=Schmidtea mediterranea OX=79327 GN=gcr11... [more]
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BLAST of GCR115 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR115 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000051770.10.000e+099.80SMESG000051770.1[more]
SMESG000051772.11.424e-10541.22SMESG000051772.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030936 ID=SMED30030936|Name=GCR115|organism=Schmidtea mediterranea sexual|type=transcript|length=1601bp
ATATTTTCTCCCGGTTTTATGCATGGATGAAATTTGTTGGCCATGGACGA
AAAATTTCTTTCTTTCAATGATTTCAAAATGTCCATCTTCATATAAAAAC
AAATACTTATCGGAGAAATGTGAAAATCCCAATATTTTCCTTTCGGACAT
TGTCGATTATAGGCCAGTAATAGCGCAAAGCCAACCAATGAACGTGCTGT
ATCGAAATATGTATTGCGCAAAATGCCATAATATTGTCAAATACGATTGG
ATTAAGGTGAATCGTAAATTGAAATGCAAAATTAATCTCAAATCCCGTAA
AATAAAAGACGAAAACTCTTACTATGCTAGGATGTTGTATAATCATCAAT
GTGAATTTGTTATTGACCAAAGACAATACAGTCAATTCTCTTGTATGATT
CCAATAGGAGTCTGTGATGGTTATTTATTAAATAATGTCGAATCTTTTTA
TGGATATATCGAGAAAAATCCAACAATAAAATGGTTTGAATTTCCAAGCT
TGAATTGCTGCAAAAATAACAGCAGGATTTGTATTGTTAATTCTGTAGGT
AGATCAAAACTTTTCTATTATTCCATCAATTCTTTATTGTACAATAACCA
AATAGAAGTTGTGAAAAATTCATCATTGAAAAATACGACATCAAATCATG
AAGATTCTTTTTTGGTTTATGCACTAGATATTGCCGCAACTTCACTGTCA
TTGGTCGCTTCGATTTTATTGATTTCTGCTTATCTAATAATTCCAGAATT
ACAAACATCAACGAGTTATATGATTGTTGCTATTGGATTCAATATCGCTT
GTTTGGAATTGACCAATCTCTTGAGCACATTCAAATGCGACTCCTGTCAA
TTTCTCGCATGCAAATTCCATGGAATATTTTCTATCTTTTTCCATATTTC
TGAAACGATTTGTATTAGTAATTACATAACAAGTTTTTGGAATGATCTCA
ACCAAATGCGTCGTCATAGTCAGAACGGGATCCATGAAAAAAGACGAAAT
CTAAAGAGAACTGTTAGATTTTATCTTTCATCTGCAGTGATTCCGCTTAT
TTTCAGTTCCAGTTATTTATTATTTGACAGGTTTGATTTCATTAATTTCA
AGGAAAAAAATAAGACTAGAATGTGTTTTTCGTTGAAAATGATTCCTTTG
ATTTGCTTCTACATAATTCCTGTATTTGTTGTTTTAATCTACACAACGAT
TGTAAATATGAAAATTTGTTTCTATTTGCATTCTTCAGTAAAAATAAGCT
CCAACGAAACTCAAAATCAAACTCGACAGCATTTTCTACTTGGACTAAAA
TTGTGTTTTATAAGTGGAATCACTTGGATTTTCAATTTGATTATGTCGGT
CTTTCCCAACAGGATTGTCCTTGATTGCGGAATGATTATATCTCACTTCA
ATGGGTTTGTCATTGTTGTATGCTTTCTGTCTGGGAGAATGGTCAGGAAA
TGCATCTTCAAGAAAGCGTCGTCTCATTAAATGGGATTTTTTTTCTACAT
TTGAACGAATATAATTAATATTTAATTTTAAAAAAATCAAATTAATAGTA
AAAGTTTTAAGGGAAAAATTCAAAAGAGATTTCTTCTATAACTATTCAAT
A
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protein sequence of SMED30030936-orf-1

>SMED30030936-orf-1 ID=SMED30030936-orf-1|Name=SMED30030936-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=493bp
YFLPVLCMDEICWPWTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDI
VDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRK
IKDENSYYARMLYNHQCEFVIDQRQYSQFSCMIPIGVCDGYLLNNVESFY
GYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQ
IEVVKNSSLKNTTSNHEDSFLVYALDIAATSLSLVASILLISAYLIIPEL
QTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHIS
ETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLI
FSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTI
VNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSV
FPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKASSH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 207..477
e-value: 1.0E-15
score: 59.7
NoneNo IPR availablePANTHERPTHR45902FAMILY NOT NAMEDcoord: 12..150
coord: 184..485
NoneNo IPR availableCDDcd139527tm_classBcoord: 230..475
e-value: 8.16031E-13
score: 66.4728
NoneNo IPR availableTMHMMTMhelixcoord: 461..478
NoneNo IPR availableTMHMMTMhelixcoord: 340..358
NoneNo IPR availableTMHMMTMhelixcoord: 429..451
NoneNo IPR availableTMHMMTMhelixcoord: 226..248
NoneNo IPR availableTMHMMTMhelixcoord: 255..277
NoneNo IPR availableTMHMMTMhelixcoord: 378..400
NoneNo IPR availableTMHMMTMhelixcoord: 292..314