MULE domain-containing protein

Overview
NameMULE domain-containing protein
Smed IDSMED30030935
Length (bp)632
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030935

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30030935 SmedASXL_072328SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30030935 SmedASXL_072328SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of MULE domain-containing protein vs. TrEMBL
Match: A0A1D2M6Z4 (Uncharacterized protein OS=Orchesella cincta OX=48709 GN=Ocin01_17935 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 5.619e-13
Identity = 40/86 (46.51%), Postives = 47/86 (54.65%), Query Frame = 3 
Query:  132 ITIMVDLELAKIRAL----PTATTTGCMFHFGQCVWWKFQA-KGFSEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFEHCTKAPFY 374
            +++  D E A   A     P AT +GC+FHFGQC W K Q  K  S  Y+ +PDFA  VK L  LAFVPP  V D FE      FY
Sbjct:  444 VSVTTDYEKAAKNAFKSVFPNATQSGCLFHFGQCFWRKIQQLKDVSSKYVTDPDFALQVKCLTALAFVPPPSVKDAFEILIADEFY 529
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BLAST of MULE domain-containing protein vs. TrEMBL
Match: J9KNP2 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 8.511e-12
Identity = 30/88 (34.09%), Postives = 47/88 (53.41%), Query Frame = 3 
Query:  126 NQITIMVDLELAKIRA----LPTATTTGCMFHFGQCVWWKFQAKGFSEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFEHCTKAPFYR 377
            N + IM D E A + A     P     GC FH GQ +W + Q  G S  Y  +P+F+  ++++L LA++P   V D FE+  +  F++
Sbjct:   13 NPLMIMADFEKAAMNAALFVYPNTKIQGCFFHLGQSIWRQIQNIGLSNKYSTDPEFSLNIRKILALAYLPENLVEDSFENILQTEFFK 100
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BLAST of MULE domain-containing protein vs. TrEMBL
Match: A0A0C2N2Y4 (MULE domain-containing protein OS=Thelohanellus kitauei OX=669202 GN=RF11_09034 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.141e-11
Identity = 33/89 (37.08%), Postives = 49/89 (55.06%), Query Frame = 3 
Query:  135 TIMVDLELAKIRA----LPTATTTGCMFHFGQCVWWKFQAKGFS-EHYINEPDFAFFVKRLLTLAFVPPQYVTDLFEHCTKAPFYRDKV 386
            +I++D E++ + A     P ++  GC FH GQC+W K Q        Y +EPDF   +K L+ LAF+PP  V   FE    +PF+ D +
Sbjct:  173 SILIDFEMSMMNAARNIFPDSSIKGCFFHLGQCIWRKIQESALVLRRYKDEPDFCLNIKTLMALAFLPPSDVIVGFETLVTSPFFADNI 261
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BLAST of MULE domain-containing protein vs. TrEMBL
Match: X1XNU4 (MULE domain-containing protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 2.188e-11
Identity = 34/87 (39.08%), Postives = 49/87 (56.32%), Query Frame = 3 
Query:  135 TIMVDLELAKIRAL----PTATTTGCMFHFGQCVWWKFQAKGF-SEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFEHCTKAPFYRD 380
            +IM D E+A I A     P     GC FHF QC+W K Q   + ++ YI++  F+  ++ LL LA+VP  +V D FE    +P+Y D
Sbjct:  301 SIMSDFEMASINAFKEVFPNVKQKGCHFHFSQCIWRKIQQIQYMAQKYISDSTFSIQIRLLLALAYVPENHVIDAFEELIDSPYYTD 387
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BLAST of MULE domain-containing protein vs. TrEMBL
Match: J9LH89 (MULE domain-containing protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 4.471e-11
Identity = 35/86 (40.70%), Postives = 46/86 (53.49%), Query Frame = 3 
Query:  135 TIMVDLELAKIRALPTA----TTTGCMFHFGQCVWWKFQAKGFSEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFEHCTKAPFYRD 380
            T+M+D E+  + AL          GC FHF Q VW   Q  G S+ Y  +  FAF +K+L  LA+VP  YV   FE+    PFYR+
Sbjct:  267 TVMLDFEIGAMTALKKEFSDIKIRGCHFHFAQSVWRHIQECGLSKQYKEDSTFAFEIKKLNALAYVPVDYVVRYFEYLVDTPFYRE 352

HSP 2 Score: 24.6386 bits (52), Expect = 4.471e-11
Identity = 12/35 (34.29%), Postives = 20/35 (57.14%), Query Frame = 2 
Query:   20 LHGFINGEISPVV*AVISSKTEKAYQNLLQKILML 124
            +HG  +  + P V A++ +K +K Y  LLQ +  L
Sbjct:  225 IHGIQSSNVLPSVYALLPNKKKKTYIRLLQSLKTL 259
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BLAST of MULE domain-containing protein vs. Planmine SMEST
Match: SMESG000070712.1 (SMESG000070712.1)

HSP 1 Score: 50.447 bits (119), Expect = 2.465e-7
Identity = 28/81 (34.57%), Postives = 39/81 (48.15%), Query Frame = 3 
Query:  135 TIMVDLELAKIRA----LPTATTTGCMFHFGQCVWWKFQAKGFSEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFEHCTKA 365
            TI  D E A I A           GC FH  Q ++ KFQ+ G  + Y N+ DFA  ++ +   AF P + V + FE   K+
Sbjct:  137 TITTDFERAAINAATECFANIKIHGCFFHNAQKIFKKFQSVGLQDRYQNDEDFALSIRMIAASAFFPIEKVIESFETLQKS 217
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BLAST of MULE domain-containing protein vs. Planmine SMEST
Match: SMESG000014782.1 (SMESG000014782.1)

HSP 1 Score: 50.447 bits (119), Expect = 2.601e-7
Identity = 26/76 (34.21%), Postives = 37/76 (48.68%), Query Frame = 3 
Query:  135 TIMVDLELAKIRA----LPTATTTGCMFHFGQCVWWKFQAKGFSEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFE 350
            TI  D E A I +           GC FH  Q ++ K Q+ G    Y N+ DFA  ++++  LAFV  + V + FE
Sbjct:  258 TITTDFERAAINSATECFANVEIHGCFFHLAQNIFRKVQSVGLQGRYQNDEDFALSIQKIAVLAFVLSENVIESFE 333
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BLAST of MULE domain-containing protein vs. Planmine SMEST
Match: SMESG000014782.1 (SMESG000014782.1)

HSP 1 Score: 50.447 bits (119), Expect = 2.692e-7
Identity = 26/76 (34.21%), Postives = 37/76 (48.68%), Query Frame = 3 
Query:  135 TIMVDLELAKIRA----LPTATTTGCMFHFGQCVWWKFQAKGFSEHYINEPDFAFFVKRLLTLAFVPPQYVTDLFE 350
            TI  D E A I +           GC FH  Q ++ K Q+ G    Y N+ DFA  ++++  LAFV  + V + FE
Sbjct:  256 TITTDFERAAINSATECFANVEIHGCFFHLAQNIFRKVQSVGLQGRYQNDEDFALSIQKIAVLAFVLSENVIESFE 331
The following BLAST results are available for this feature:
BLAST of MULE domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1D2M6Z45.619e-1346.51Uncharacterized protein OS=Orchesella cincta OX=48... [more]
J9KNP28.511e-1234.09Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
A0A0C2N2Y42.141e-1137.08MULE domain-containing protein OS=Thelohanellus ki... [more]
X1XNU42.188e-1139.08MULE domain-containing protein OS=Acyrthosiphon pi... [more]
J9LH894.471e-1140.70MULE domain-containing protein OS=Acyrthosiphon pi... [more]
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BLAST of MULE domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MULE domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000070712.12.465e-734.57SMESG000070712.1[more]
SMESG000014782.12.601e-734.21SMESG000014782.1[more]
SMESG000014782.12.692e-734.21SMESG000014782.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030935 ID=SMED30030935|Name=MULE domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=632bp
CTTAATGTAGTAATAAAATCTTCATGGTTTCATTAATGGCGAAATTTCCC
CGGTAGTATAGGCAGTAATATCTTCAAAAACGGAAAAAGCATATCAAAAT
CTACTTCAGAAAATTCTGATGTTGAAACCAGATTACAATTATGGTTGATC
TCGAACTAGCAAAAATTAGAGCATTGCCGACAGCAACAACCACCGGATGC
ATGTTTCACTTTGGTCAATGCGTTTGGTGGAAATTTCAAGCGAAAGGATT
CTCGGAACATTACATAAATGAACCTGATTTCGCCTTTTTCGTTAAACGAC
TTCTGACTCTAGCTTTCGTTCCACCACAGTATGTTACTGATTTGTTTGAA
CATTGCACCAAAGCTCCATTTTATCGGGACAAAGTCATAGGTAGACTAAG
AAGATGACCACCAAGATTGTCCATTATTATTTACTTATTTACGATATTGA
TCTACATATTTGACTTTAACACGATATTGACCTACTTATTTGACATTAAA
ACGATATTGACCTACTCATTTGACCTTAAAGCGATATTATCCTGAATACT
TGACCTTAACACAACCTTGACCTACTTATTTGACCTTAAAACGATATTAA
CCTACATATTTGACCTTAAAACGATATTGACT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell