60S ribosomal protein L27
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030908 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15
Homology
BLAST of 60S ribosomal protein L27 vs. Ensembl Human
Match: RPL27 (ribosomal protein L27 [Source:HGNC Symbol;Acc:HGNC:10328]) HSP 1 Score: 106.301 bits (264), Expect = 2.840e-29 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Human
Match: RPL27 (ribosomal protein L27 [Source:HGNC Symbol;Acc:HGNC:10328]) HSP 1 Score: 106.301 bits (264), Expect = 2.840e-29 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Human
Match: RPL27 (ribosomal protein L27 [Source:HGNC Symbol;Acc:HGNC:10328]) HSP 1 Score: 106.301 bits (264), Expect = 2.840e-29 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Human
Match: RPL27 (ribosomal protein L27 [Source:HGNC Symbol;Acc:HGNC:10328]) HSP 1 Score: 86.2705 bits (212), Expect = 2.344e-21 Identity = 72/118 (61.02%), Postives = 93/118 (78.81%), Query Frame = 1 Query: 151 AGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 AGR + K+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 27 AGRAGLEFKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 144
BLAST of 60S ribosomal protein L27 vs. Ensembl Celegans
Match: rpl-27 (60S ribosomal protein L27 [Source:UniProtKB/Swiss-Prot;Acc:P91914]) HSP 1 Score: 98.9821 bits (245), Expect = 7.495e-27 Identity = 70/128 (54.69%), Postives = 98/128 (76.56%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ G+YAGRKAV+VK DEG SD++Y HA++ GI YP +VTK MGKKKI KR+K+KPF+K+++Y HLL TR+++D+ + T +NK+ K K+ A E KSK EE+YKTGKN+WF+ KLRF Sbjct: 9 KVVLVLRGKYAGRKAVVVKQQDEGVSDRTYPHAIIAGIDRYPLKVTKDMGKKKIEKRNKLKPFLKVVSYTHLLPTRYSVDVAFDKTNINKEALKAPSKKRKALVEVKSKFEERYKTGKNKWFFTKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Celegans
Match: rpl-27 (60S ribosomal protein L27 [Source:UniProtKB/Swiss-Prot;Acc:P91914]) HSP 1 Score: 98.9821 bits (245), Expect = 7.495e-27 Identity = 70/128 (54.69%), Postives = 98/128 (76.56%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ G+YAGRKAV+VK DEG SD++Y HA++ GI YP +VTK MGKKKI KR+K+KPF+K+++Y HLL TR+++D+ + T +NK+ K K+ A E KSK EE+YKTGKN+WF+ KLRF Sbjct: 9 KVVLVLRGKYAGRKAVVVKQQDEGVSDRTYPHAIIAGIDRYPLKVTKDMGKKKIEKRNKLKPFLKVVSYTHLLPTRYSVDVAFDKTNINKEALKAPSKKRKALVEVKSKFEERYKTGKNKWFFTKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Fly
Match: RpL27 (gene:FBgn0039359 transcript:FBtr0331360) HSP 1 Score: 93.9745 bits (232), Expect = 8.860e-25 Identity = 65/129 (50.39%), Postives = 93/129 (72.09%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTI-DLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 +IV++ +GRYAGRKA+IVK++D+GT +K +GHALV GI YP++VTK MGK K+ K+SK+KPF+K +NYNHL+ TR+T D+ + KD +K + + + K E YK GKN+WF++KLRF Sbjct: 9 KIVIVLSGRYAGRKAIIVKTHDDGTPEKPFGHALVAGIDRYPRKVTKKMGKNKLKKKSKVKPFLKSLNYNHLMPTRYTAHDISFEKL--SPKDLKDPVKRKTHRFQTRVKFESVYKEGKNKWFFQKLRF 135
BLAST of 60S ribosomal protein L27 vs. Ensembl Fly
Match: RpL27 (gene:FBgn0039359 transcript:FBtr0331362) HSP 1 Score: 93.9745 bits (232), Expect = 8.860e-25 Identity = 65/129 (50.39%), Postives = 93/129 (72.09%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTI-DLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 +IV++ +GRYAGRKA+IVK++D+GT +K +GHALV GI YP++VTK MGK K+ K+SK+KPF+K +NYNHL+ TR+T D+ + KD +K + + + K E YK GKN+WF++KLRF Sbjct: 9 KIVIVLSGRYAGRKAIIVKTHDDGTPEKPFGHALVAGIDRYPRKVTKKMGKNKLKKKSKVKPFLKSLNYNHLMPTRYTAHDISFEKL--SPKDLKDPVKRKTHRFQTRVKFESVYKEGKNKWFFQKLRF 135
BLAST of 60S ribosomal protein L27 vs. Ensembl Fly
Match: RpL27 (gene:FBgn0039359 transcript:FBtr0331361) HSP 1 Score: 93.9745 bits (232), Expect = 8.860e-25 Identity = 65/129 (50.39%), Postives = 93/129 (72.09%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTI-DLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 +IV++ +GRYAGRKA+IVK++D+GT +K +GHALV GI YP++VTK MGK K+ K+SK+KPF+K +NYNHL+ TR+T D+ + KD +K + + + K E YK GKN+WF++KLRF Sbjct: 9 KIVIVLSGRYAGRKAIIVKTHDDGTPEKPFGHALVAGIDRYPRKVTKKMGKNKLKKKSKVKPFLKSLNYNHLMPTRYTAHDISFEKL--SPKDLKDPVKRKTHRFQTRVKFESVYKEGKNKWFFQKLRF 135
BLAST of 60S ribosomal protein L27 vs. Ensembl Fly
Match: RpL27 (gene:FBgn0039359 transcript:FBtr0084932) HSP 1 Score: 93.9745 bits (232), Expect = 8.860e-25 Identity = 65/129 (50.39%), Postives = 93/129 (72.09%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTI-DLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 +IV++ +GRYAGRKA+IVK++D+GT +K +GHALV GI YP++VTK MGK K+ K+SK+KPF+K +NYNHL+ TR+T D+ + KD +K + + + K E YK GKN+WF++KLRF Sbjct: 9 KIVIVLSGRYAGRKAIIVKTHDDGTPEKPFGHALVAGIDRYPRKVTKKMGKNKLKKKSKVKPFLKSLNYNHLMPTRYTAHDISFEKL--SPKDLKDPVKRKTHRFQTRVKFESVYKEGKNKWFFQKLRF 135
BLAST of 60S ribosomal protein L27 vs. Ensembl Zebrafish
Match: rpl27 (ribosomal protein L27 [Source:NCBI gene;Acc:325618]) HSP 1 Score: 107.457 bits (267), Expect = 7.278e-30 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTATMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Zebrafish
Match: rpl27 (ribosomal protein L27 [Source:NCBI gene;Acc:325618]) HSP 1 Score: 107.457 bits (267), Expect = 7.278e-30 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTATMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Xenopus
Match: ninj1 (ninjurin 1 [Source:Xenbase;Acc:XB-GENE-959247]) HSP 1 Score: 106.301 bits (264), Expect = 3.028e-29 Identity = 80/128 (62.50%), Postives = 105/128 (82.03%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKK+IAKRSKIK FVK+ NYNHL+ TR+++D+ L+ VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYAGRKAVIVKNVDDGTSDRQYSHALVAGIDRYPRKVTATMGKKRIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKAVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Mouse
Match: Rpl27 (ribosomal protein L27 [Source:MGI Symbol;Acc:MGI:98036]) HSP 1 Score: 106.301 bits (264), Expect = 1.950e-29 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Mouse
Match: Rpl27 (ribosomal protein L27 [Source:MGI Symbol;Acc:MGI:98036]) HSP 1 Score: 106.301 bits (264), Expect = 1.950e-29 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. UniProt/SwissProt
Match: sp|Q7ZV82|RL27_DANRE (60S ribosomal protein L27 OS=Danio rerio OX=7955 GN=rpl27 PE=2 SV=3) HSP 1 Score: 107.457 bits (267), Expect = 5.494e-29 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTATMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. UniProt/SwissProt
Match: sp|P61359|RL27_HIPCM (60S ribosomal protein L27 OS=Hippocampus comes OX=109280 GN=rpl27 PE=2 SV=2) HSP 1 Score: 107.071 bits (266), Expect = 9.237e-29 Identity = 81/128 (63.28%), Postives = 104/128 (81.25%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTAPMGKKKIAKRSKIKAFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKSKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. UniProt/SwissProt
Match: sp|Q90YU1|RL27_ICTPU (60S ribosomal protein L27 OS=Ictalurus punctatus OX=7998 GN=rpl27 PE=2 SV=3) HSP 1 Score: 106.686 bits (265), Expect = 1.087e-28 Identity = 80/128 (62.50%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT +MGKKK+AKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVSGIDRYPRKVTATMGKKKVAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTIVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. UniProt/SwissProt
Match: sp|Q9XSU7|RL27_CANLF (60S ribosomal protein L27 OS=Canis lupus familiaris OX=9615 GN=RPL27 PE=2 SV=3) HSP 1 Score: 106.686 bits (265), Expect = 1.110e-28 Identity = 82/128 (64.06%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EEKYKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEEKYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. UniProt/SwissProt
Match: sp|P61354|RL27_RAT (60S ribosomal protein L27 OS=Rattus norvegicus OX=10116 GN=Rpl27 PE=2 SV=2) HSP 1 Score: 106.301 bits (264), Expect = 1.364e-28 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRY+GRKAVIVK+ D+GTSD+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. TrEMBL
Match: Q6JWX4 (60S ribosomal protein L27 OS=Oikopleura dioica OX=34765 GN=RL27 PE=3 SV=1) HSP 1 Score: 115.161 bits (287), Expect = 1.747e-29 Identity = 78/128 (60.94%), Postives = 101/128 (78.91%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V+L AGRYAGRKA+IVK D+G D+ YGHALV GI YP++VTK MGKK+I KR +IKPFVK+INYNHL+ TR+++D+ LN T VNK+ KDK K+ A K + EE+YKTGKN+WF++KLRF Sbjct: 9 KVVILLAGRYAGRKALIVKQQDDGVQDRQYGHALVAGIARYPRKVTKDMGKKRITKRCRIKPFVKVINYNHLMPTRYSVDVPLNKTVVNKEGIKDKAGKRKAVESVKLQFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. TrEMBL
Match: A0A1B6HSV9 (60S ribosomal protein L27 OS=Homalodisca liturata OX=320908 GN=g.9511 PE=3 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 4.454e-28 Identity = 79/129 (61.24%), Postives = 104/129 (80.62%), Query Frame = 1 Query: 118 NRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 N++V++ +GRYAG+KAVIVK++DEGTSDK YGHALV GI YP++V K MGKKKI KRSKIKPF+K++NYNHL+ TR+T+D L N K+N KD KK + + + +LE+KYKTGKN+WF++KLRF Sbjct: 8 NKVVLVLSGRYAGKKAVIVKNFDEGTSDKQYGHALVAGIDRYPRKVHKRMGKKKIHKRSKIKPFLKVMNYNHLMPTRYTLD--LENEKINHKDLKDPMKKKKLRFKVRVRLEDKYKTGKNKWFFQKLRF 134
BLAST of 60S ribosomal protein L27 vs. TrEMBL
Match: A0A553P9W1 (60S ribosomal protein L27 OS=Tigriopus californicus OX=6832 GN=TCAL_08319 PE=3 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 5.143e-28 Identity = 72/128 (56.25%), Postives = 101/128 (78.91%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ +GR+AGRKAVIVK DEGT+DK +GHALV GI YP+ VTK M KKKI RSK+KPF+K++NYNHL+ TR++++LG + +NK+ KD KK A+ +++ EE+YK+GKNRWF++KLRF Sbjct: 9 KVVLVLSGRFAGRKAVIVKPTDEGTADKPFGHALVAGIDRYPRMVTKRMSKKKIKMRSKVKPFLKVVNYNHLMPTRYSVELGFDKANLNKETLKDPMKKKKARNMVRTQFEERYKSGKNRWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. TrEMBL
Match: S4RNM4 (60S ribosomal protein L27 OS=Petromyzon marinus OX=7757 PE=3 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 5.489e-28 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ DEGT+D+ YGHALV GI YP++VT MGKKK+AKRSK+K FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYAGRKAVIVKNQDEGTADRPYGHALVAGIDRYPRKVTARMGKKKLAKRSKVKSFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. TrEMBL
Match: A0A4Y2KUM3 (60S ribosomal protein L27 OS=Araneus ventricosus OX=182803 GN=Rpl27 PE=3 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 5.795e-28 Identity = 76/128 (59.38%), Postives = 104/128 (81.25%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ GRYAGRKAV+VK+ DEGTSDK YGHALV GI YP++VT+ MGKKK+ KRSKIKPFVK++NYNHL+ TR+ +D+ + + VNK+ KD ++ A+ + K+K EE+YKTGK+RWF++KLRF Sbjct: 9 KVVLVLNGRYAGRKAVVVKNNDEGTSDKPYGHALVAGIDRYPRKVTRRMGKKKLEKRSKIKPFVKVLNYNHLMPTRYMVDIAFDKSIVNKEVLKDPSKRRKARRDVKAKFEERYKTGKSRWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Cavefish
Match: rpl27 (ribosomal protein L27 [Source:NCBI gene;Acc:103038111]) HSP 1 Score: 105.145 bits (261), Expect = 4.373e-29 Identity = 80/128 (62.50%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT +MGKK+IAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNVDDGTTDRPYSHALVSGIDRYPRKVTTNMGKKRIAKRSKIKAFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Sea Lamprey
Match: rpl27 (ribosomal protein L27 [Source:NCBI gene;Acc:325618]) HSP 1 Score: 111.309 bits (277), Expect = 4.217e-32 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ DEGT+D+ YGHALV GI YP++VT MGKKK+AKRSK+K FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVLVLAGRYAGRKAVIVKNQDEGTADRPYGHALVAGIDRYPRKVTARMGKKKLAKRSKVKSFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Yeast
Match: RPL27B (Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication [Source:SGD;Acc:S000002879]) HSP 1 Score: 100.138 bits (248), Expect = 6.379e-28 Identity = 64/130 (49.23%), Postives = 91/130 (70.00%), Query Frame = 1 Query: 115 GNRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++ V+ GRYAG+K VIVK +DEG+ +GHALV GI YP +VTK G KK+AKR+KIKPF+K++NYNHLL TR+T+D+ + V+ + F+ ++ AK K EE+++ GKN+WF+ KLRF Sbjct: 7 AGKVAVVVRGRYAGKKVVIVKPHDEGSKSHPFGHALVAGIERYPSKVTKKHGAKKVAKRTKIKPFIKVVNYNHLLPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Yeast
Match: RPL27A (Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication [Source:SGD;Acc:S000001052]) HSP 1 Score: 98.9821 bits (245), Expect = 1.760e-27 Identity = 64/130 (49.23%), Postives = 91/130 (70.00%), Query Frame = 1 Query: 115 GNRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++ V+ GRYAG+K VIVK +DEG+ +GHALV GI YP +VTK G KK+AKR+KIKPF+K++NYNHLL TR+T+D+ + V+ + F+ ++ AK K EE+++ GKN+WF+ KLRF Sbjct: 7 AGKVAVVVRGRYAGKKVVIVKPHDEGSKSHPFGHALVAGIERYPLKVTKKHGAKKVAKRTKIKPFIKVVNYNHLLPTRYTLDVEAFKSVVSTETFEQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Nematostella
Match: EDO42969 (60S ribosomal protein L27 [Source:UniProtKB/TrEMBL;Acc:A7S072]) HSP 1 Score: 106.686 bits (265), Expect = 5.353e-30 Identity = 77/128 (60.16%), Postives = 104/128 (81.25%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ GRYAG+KA+I+K+YD+G+SDK YGHALV G+ YP +VTK MGKK+ AKRSK+KPFVK+ NYNHL+ TR+++D+ L+ VNKD F+D KK A E KS LEE+YK+GKN+WF++KLRF Sbjct: 9 KVVLVLRGRYAGKKALIIKNYDDGSSDKPYGHALVAGVARYPLKVTKRMGKKRTAKRSKVKPFVKVFNYNHLMPTRYSVDVPLDKQVVNKDVFRDPALKKKALREVKSTLEERYKSGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Ensembl Medaka
Match: rpl27 (ribosomal protein L27 [Source:NCBI gene;Acc:101168542]) HSP 1 Score: 107.071 bits (266), Expect = 7.948e-30 Identity = 81/128 (63.28%), Postives = 106/128 (82.81%), Query Frame = 1 Query: 121 RIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 ++V++ AGRYAGRKAVIVK+ D+GT+D+ Y HALV GI YP++VT +MGKKKIAKRSKIK FVK+ NYNHL+ TR+++D+ L+ T VNKD F+D K+ A+ EAK K EE+YKTGKN+WF++KLRF Sbjct: 9 KVVMVLAGRYAGRKAVIVKNIDDGTADRPYSHALVAGIDRYPRKVTTTMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136
BLAST of 60S ribosomal protein L27 vs. Planmine SMEST
Match: SMESG000045074.1 (SMESG000045074.1) HSP 1 Score: 161.77 bits (408), Expect = 2.239e-51 Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 1 Query: 115 GNRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTXXXXXXXXXXXXXXXPFVKIINYNHLLTTRFTIDLGLNNTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGKNRWFYRKLRF 504 GNRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTKSMGKKKIAKRSKIKPFVKIINYNHLLTTRFTIDLGLNNTKVNKDCFKDKKSKKVAKAEAKSKLEEKYKTGKNRWFYRKLRF Sbjct: 6 GNRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKEVTKSMGKKKIAKRSKIKPFVKIINYNHLLTTRFTIDLGLNNTKVNKDCFKDKKSKKVAKAEAKSKLEEKYKTGKNRWFYRKLRF 135 The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L27 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 4
BLAST of 60S ribosomal protein L27 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of 60S ribosomal protein L27 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 4
BLAST of 60S ribosomal protein L27 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of 60S ribosomal protein L27 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of 60S ribosomal protein L27 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of 60S ribosomal protein L27 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 60S ribosomal protein L27 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 60S ribosomal protein L27 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of 60S ribosomal protein L27 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of 60S ribosomal protein L27 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of 60S ribosomal protein L27 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of 60S ribosomal protein L27 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of 60S ribosomal protein L27 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030908 ID=SMED30030908|Name=60S ribosomal protein L27|organism=Schmidtea mediterranea sexual|type=transcript|length=545bpback to top protein sequence of SMED30030908-orf-1 >SMED30030908-orf-1 ID=SMED30030908-orf-1|Name=SMED30030908-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp MKLMKGNRIVVLTAGRYAGRKAVIVKSYDEGTSDKSYGHALVVGIRSYPKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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