Ubox domain containing protein

Overview
NameUbox domain containing protein
Smed IDSMED30030897
Length (bp)1808
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ubox domain containing protein (SMED30030897) t-SNE clustered cells

Violin plots show distribution of expression levels for Ubox domain containing protein (SMED30030897) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ubox domain containing protein (SMED30030897) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ubox domain containing protein (SMED30030897) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030897

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30030897h1SMcG0004322 dd_Smed_v4_2397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30030897h1SMcG0004322 dd_Smed_v4_2397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30030897h1SMcG0004322 dd_Smed_v4_2397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Ubox domain containing protein vs. TrEMBL
Match: K1P710 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10008445 PE=4 SV=1)

HSP 1 Score: 449.129 bits (1154), Expect = 1.062e-148
Identity = 223/519 (42.97%), Postives = 328/519 (63.20%), Query Frame = 1
Query:  157 DFLSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNVCFYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVF 1713
            D  ++P  YF  + + + H   QN  R RY AT+  L    +++  +A+++A+  AWARKD ++A  FL  +  F   EV KA+ LLD  RQ +  E K   +KML+ +K   VK  T   L   I  L+K K  +GS SGA+C+ +R W   F   EL F+ ++F    WK++ADI HF+PQK+F SL WFLPYC+G+SAP  +TV R  ++N+ENVNE+L+   +P+  +   K  LT+ SK  I   ED +   +WWYE+L C +V++ +  RL   + +    GKL+ERLM+ K +N  FY  L++IAE KL +    LE P+ V+ DASSSM VA++T+TII+  LT  T+A+L FFND +  P+K PT++ +V++LALNT AS  T+ AA+L+ +Y+ K  LKT+IMVTDEEENT++Q+  F  L+ KY +EV P +L+F+SFL  QHS+GQMV   +A+N+D  Q VF+  +PD++KLD + G +S     F + +   E  +  + V+ VF
Sbjct:    4 DLETNPQDYFTGNVTVEQHYNGQNENRERYAATMAVLQENGVDVAQNARIRALAAAWARKDFKLAESFLGNRDNFGLNEVLKALQLLDAGRQARVLEKK---MKMLQVSKN-KVKSITMGKLKSDIDNLNKMKSPYGSASGAVCKHIRRWVQHFTAKELVFYAIHFPKEPWKKLADICHFHPQKDFPSLDWFLPYCYGQSAPADTTVGRMVSMNAENVNELLEGLNIPYLTVKPHKDKLTNVSKGRIADREDKLDTVLWWYEELACPEVEDAVQRRLEKGDPVTLPIGKLLERLMVFKSRNAKFYHDLLKIAEYKLKQIKLPLESPVFVMGDASSSMSVAVKTSTIIAGILTAITSAKLSFFNDTHREPDKVPTNVEEVLELALNTPASGCTANAASLYPYYERKEVLKTLIMVTDEEENTSFQNFRFKELYTKYCEEVYPARLVFVSFLHFQHSKGQMVTRFKASNMDVMQCVFHRSQPDLTKLDKLFGLLSAETQSFEEQIKMTEEQVRKQGVDKVF 518          
BLAST of Ubox domain containing protein vs. TrEMBL
Match: A0A267DXV1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020745g1 PE=4 SV=1)

HSP 1 Score: 437.187 bits (1123), Expect = 2.543e-143
Identity = 215/524 (41.03%), Postives = 320/524 (61.07%), Query Frame = 1
Query:  157 DFLSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNVC--FYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVFGNI 1722
            D   D +++FL  TSF+ H  AQ + R+RY A +  L    +EI  +A+V+A+  AWAR D  +A+  L ++  F   EV KA  +LD  R+I+  E ++ K     +++    K      +   I  LS  +   GS SGA CR V+ W   F+  EL++F ++     WK++ADI H +P  +F +  WFLP+CFG  AP+ +  ++   +  ENVNEIL    LPF +I +F +++++++K  +V+N+D V R IWWYED++C +VD  I ERL + EK+   YGKLMERL++    +    FYP L+ +AE KL E +  LE P+ V+ DASSSM+VAIRTATIISS L+      LIFFN   W PE NP +I +V+ LA +T+AS  T+ AAAL+ +Y+ K  ++T I+VTDEEENT++Q   F  LF++Y  EV   KL+F+SFL  QHS+GQMV       ++  Q+V N  +PD++KLDSI+G++ +  S F + +  +   +G E +  VF  +
Sbjct:   58 DIAGDSSAFFLGVTSFEQHTRAQTSNRSRYAAAIAVLTKSGVEINQNARVRALAAAWARNDPALADGLLKLRGSFGVAEVLKAATMLDSGRKIREWEKRLAK----AQSQPGGAKKKFVSKVKYNIDNLSAIRPAQGSASGAFCRHVKRWVRTFSLAELDYFALHMSKDPWKKLADICHLSPSIDFPAAPWFLPFCFGAEAPDGTLAKQCSIITPENVNEILAERALPFSEIRRFLEHMSNQTKARVVRNDDRVDRGIWWYEDVKCPEVDAAIEERLRNGEKVTLTYGKLMERLLLFSGMSPVPSFYPMLIPVAEHKLQETTLNLESPVVVIGDASSSMNVAIRTATIISSLLSAIIKCDLIFFNVKSWSPEANPRTIEEVLSLARSTKASGCTAHAAALYPYYERKEIVRTFIVVTDEEENTDFQGTRFFPLFERYRQEVFDAKLVFVSFLRQQHSRGQMVYKAAELGLNVQQFVLNGSRPDLNKLDSILGRLGVQCSTFEEEVQKMTDRIGTEGLREVFDQL 577          
BLAST of Ubox domain containing protein vs. TrEMBL
Match: A0A210QK84 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT23956 PE=4 SV=1)

HSP 1 Score: 425.631 bits (1093), Expect = 1.269e-138
Identity = 219/563 (38.90%), Postives = 337/563 (59.86%), Query Frame = 1
Query:  103 PIQKLSSAIDNEEILKEYDFLSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDL--ECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLK------PKNVCFYPKLME---------------IAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVFGNI 1722
            P +  SS  D++  +KE    +D  ++F+ + S   H  AQN    RY A +  L    +EI+ +A+++A+  AWARKD +++N FL  +  F  VEV KA+ L+D  RQI+A E ++  L++     K++ K  T   L   I  L+  K  FG+ SGA+C+ +R W   F   ELEF+ + F    W+++AD+ HFNP+K+F +L+WFLPYCFGK APE S   R ++L +EN+NE++K  P+P+    + K   T ESK  I   E  +   +W+YED+   C  VD+II +R+ + E+++   GKLM+RL+MLK      P  + +  KL                     +L E S  LE PI V  DAS SM++AIRT+ IIS  L    +A+LIFFN+++  P   PT+I +V++LA+ T+A   TSPAA+LW +Y+ K  +KT IMVTDEEEN+ Y+++ F  L++KY DEV P +L+F+SFL  QH++GQMV  L++   +  Q  F+  +PD++KLD + G +S  +  F + + A+E+S+ ++ +  VF  +
Sbjct:   29 PTEGTSS--DSQVEVKELSVDTDLQAFFVGNASVAQHYAAQNQNTQRYAAGMAVLQKNGVEIVQNARIRALAAAWARKDFDLSNAFLKDRDNFGLVEVLKALQLIDSGRQIRAKEKQLRILQI--STNKVSPK--TMGKLKSDIDNLNALKPPFGTASGAVCKHIRRWVQTFTTEELEFYSLYFPKQPWQKLADLCHFNPEKDFPALTWFLPYCFGKDAPEGSMTYRCQHLTTENINEVIKEFPIPYSLAKQHKDGFTPESKARIAAYEPKLDTILWYYEDIGQNCSDVDKIIADRMKAGEEVKLQLGKLMDRLLMLKMYRENIPSKLYYGQKLERSVDENKAPFIQQLAVYGNKRLKEISLSLEAPIVVSGDASGSMEIAIRTSVIISGILAAICSAKLIFFNNDFRYPSSVPTNIEEVLELAIETKAGGGTSPAASLWPYYEKKEVIKTFIMVTDEEENSGYKNHRFAELYQKYYDEVYPMRLVFVSFLRDQHAEGQMVRELKSKGHNPLQVKFHQTQPDLTKLDKMFGTLSSESLTFEEEVTAMESSIRDQGLANVFQKV 585          
BLAST of Ubox domain containing protein vs. TrEMBL
Match: R7U0Y5 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_197625 PE=4 SV=1)

HSP 1 Score: 425.246 bits (1092), Expect = 6.141e-138
Identity = 221/532 (41.54%), Postives = 330/532 (62.03%), Query Frame = 1
Query:  163 LSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNV----CFYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEEN----TNYQD-----NDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANI--DTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVF 1713
            + D   YF+  T FD H  AQ   +NRYLA ++ L    +E+  +AKVKA++ AWAR D+ M   FLS ++ F  VE+ KA+ LLD  R+++A E K+ +L+M    K    KL   K+    I  L+  KL  GS SGA+C+Q++NWA  F+ TELE + ++F T  WK+++DI HFNP+K+F SL WFLP+CFG  APE S V + +NLN++  NE++    LPF  + +F ++LT+ESK  I +  D + + +WWYE+L C  VDE I  RL   E+    YGKLMERL+ +    +     F  +L+ +A+ +LS+ +  L+ P+ V+ D S+SMDVAIRT+TII+S +T   +A+L+FF++        PT I  V+++A+  +   ST PAA+L  FY+ K  +KT  +VTDEEEN     + +D     + F  LFKKY +E+ P KL+FISFL  QHS+G+MV+AL+   +  D TQ+ F+S +PD+SKLD + G +S  +S F   ++ +E  +    ++  F
Sbjct:  102 MEDLKKYFVEETHFDQHYAAQEENKNRYLAGMVVLEENGVEVSTNAKVKALSAAWARGDRAMVKRFLSSRESFGLVEILKALTLLDTSRRVRAEEKKLKRLQMQTNGKVNAKKLGKLKS---NIDNLNVNKLKCGSASGAICKQLKNWARSFSATELENYALHFPTEPWKKISDICHFNPKKDFPSLPWFLPFCFGSPAPEGSMVAQCRNLNADAANEMINKYSLPFSYVKEFSEHLTEESKAKIART-DTLDQVLWWYENLRCPAVDEEIRRRLLGGEQPTLAYGKLMERLLTITLIGIQAEGHFIQQLIPLAQKRLSDINFKLQAPVVVIGDRSASMDVAIRTSTIIASLITAICSARLVFFDNENMEAPFIPTDIEKVLEMAVEIKTGASTIPAASLLPFYEAKEIVKTFFIVTDEEENGEVAVSGKDGTVSYDRFAPLFKKYREEIFPAKLVFISFLRGQHSEGEMVSALKCIGVTEDVTQFKFDSSRPDLSKLDKLFGILSTDSSDFEQRVIEVENEISTAEIQPDF 629          
BLAST of Ubox domain containing protein vs. TrEMBL
Match: W4XWX0 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 412.149 bits (1058), Expect = 1.595e-133
Identity = 207/527 (39.28%), Postives = 314/527 (59.58%), Query Frame = 1
Query:   85 SKTRRKPIQKLSSAIDNEEILKEYDFLSDPTSYFLNS-TSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNV--CFYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEV-GPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRF 1653
            S   ++P Q +      E    + DF   P   F +S  +F+ H  AQ +YRN YLAT+  L+   + I    KV+A+  AWARKD  +A  FL   + F   E+ KA+NLLD  R+++  E ++ +L+ ++  +K+ V       +   I  L+  K   GSVSGAL R +R W       +LEF+ +++    WK +ADI H NP+K+F    WFL YCFG+ AP  S V +   +N+EN+NE++K   +P+  I +F  NLT+ESK  I   E+ V   +W+YEDL C +VDE+I  R+   E +    GKL+ERL+++K + +  C    L+  A  +L      LE P+ +L D S SM+VAI+T+TII+  LT  T+A+L FF+   ++PEK P +I++++++A   +    T+PAA LW +++ K  +KT ++VTDEEEN+N     F  LFKKY +EV    KL+FISFL SQH++GQMV+ L A  I   Q+ F+ ++PD++KLDS++G +S S + F
Sbjct:   34 SGQGQQPDQDIDEWNLTETATSDQDFEKRPELLFTSSKVTFEQHFAAQESYRNIYLATLKVLLANGVSISQGIKVRALAVAWARKDHALAETFLQDSETFTMAEILKALNLLDAGRRMREKERRLRQLQ-VQIQEKVKVNPKKMSKVKSDIDNLNAAKPKVGSVSGALSRHIRRWVRSLTSAQLEFYALHYPKEPWKTLADICHLNPKKDFPEKPWFLSYCFGEPAPSESLVAKCAGMNAENINELVKGVDVPYSHIKQFTLNLTEESKERIASYEEKVDTLLWYYEDLHCPKVDEVICRRISDGEAVTLPSGKLIERLLLMKTQGIGTCVMESLIPEAAKRLKAMKVSLEAPVAILGDRSPSMEVAIKTSTIIAGLLTAITDAKLSFFSYKDYVPEKIPKTITEILEMASTLRTDGGTAPAAGLWPYFRRKEVIKTFVVVTDEEENSNNNGYSFQALFKKYQEEVYSDVKLVFISFLRSQHAEGQMVSQLTADGIHPMQFKFSLVRPDLTKLDSVLGLLSTSTADF 559          
BLAST of Ubox domain containing protein vs. Planmine SMEST
Match: SMESG000054668.1 (SMESG000054668.1)

HSP 1 Score: 1151.35 bits (2977), Expect = 0.000e+0
Identity = 557/558 (99.82%), Postives = 558/558 (100.00%), Query Frame = 1
Query:   55 MAPRKLKFHSSKTRRKPIQKLSSAIDNEEILKEYDFLSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNVCFYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVFGNIGQ 1728
            MAPRKLKFHSSKTRRKPIQKLSSAIDNEE+LKEYDFLSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNVCFYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVFGNIGQ
Sbjct:    1 MAPRKLKFHSSKTRRKPIQKLSSAIDNEEVLKEYDFLSDPTSYFLNSTSFDAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMANVFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKLSTYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNFDTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSENVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLECCQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNVCFYPKLMEIAESKLSEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNYWIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTDEEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAANIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVETVFGNIGQ 558          
The following BLAST results are available for this feature:
BLAST of Ubox domain containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1P7101.062e-14842.97Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A267DXV12.543e-14341.03Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A210QK841.269e-13838.90Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
R7U0Y56.141e-13841.54Uncharacterized protein OS=Capitella teleta OX=283... [more]
W4XWX01.595e-13339.28Uncharacterized protein OS=Strongylocentrotus purp... [more]
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BLAST of Ubox domain containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubox domain containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000054668.10.000e+099.82SMESG000054668.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030897 ID=SMED30030897|Name=Ubox domain containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1808bp
AAATTCAATATTTTATTAAATATTGAAAATTGTTTTCTGTGAAATTTATA
TTTAATGGCTCCTAGAAAATTGAAATTTCACTCATCCAAAACTCGTCGTA
AGCCAATTCAGAAATTAAGTTCTGCTATAGATAATGAGGAAATTCTAAAG
GAATATGATTTCTTATCAGATCCAACGTCTTATTTTTTGAACAGTACATC
ATTTGATGCTCATCTTGAAGCTCAAAACACTTATAGGAATAGGTATTTAG
CCACTGTTCTGCAGTTGGTTAATATGAAAATCGAAATTCTTCCGAGTGCA
AAAGTCAAAGCAATTACCTGTGCATGGGCGAGAAAAGACCAAGAAATGGC
AAATGTTTTTCTGTCGATGAAAAAAATATTTAATTTCGTTGAAGTAGCGA
AAGCAGTAAATCTACTTGACATTCCTCGTCAAATTAAGGCAACTGAAAAT
AAAATCAATAAGCTAAAGATGTTAGAAGAAAATAAGAAAATTACTGTTAA
GTTGTCCACGTATAAGACTCTTGCGAAAAAAATATCAACTCTTAGCAAAC
AGAAATTGAATTTTGGATCCGTGAGTGGAGCTCTTTGTCGACAAGTGAGA
AATTGGGCGTCGAAATTCAACGAAACCGAGTTGGAATTTTTTTTAATGAA
TTTCGACACAACAGTTTGGAAAGAAATGGCAGACATCGTTCATTTTAATC
CACAAAAGAACTTCTCAAGTCTTTCTTGGTTTCTTCCGTACTGTTTTGGA
AAATCAGCGCCGGAAATTTCGACAGTTGAAAGAACGAAAAATCTCAATTC
AGAAAATGTCAATGAGATTTTAAAATCAACTCCATTGCCGTTTCATAAAA
TTAGTAAATTCAAACAAAATCTCACAGATGAATCGAAAGTGATTATCGTA
AAAAATGAAGACAATGTCGGCCGGGCAATTTGGTGGTATGAAGATTTGGA
ATGCTGTCAGGTTGATGAGATAATCATGGAAAGACTTTGCAGCAATGAAA
AACTGGAATTCGGCTACGGAAAATTGATGGAAAGACTGATGATGCTGAAA
CCGAAAAATGTTTGCTTCTATCCGAAACTAATGGAAATTGCCGAATCAAA
ACTAAGCGAATATTCTCACTGTCTCGAAGGTCCAATTGGAGTATTGGCTG
ACGCGTCCTCATCAATGGATGTTGCCATTAGAACTGCGACAATTATTTCT
TCGTTCTTGACTGTGTTCACGAATGCCCAATTGATTTTCTTCAATGATAA
TTATTGGATACCGGAAAAGAATCCTACTTCGATATCTGATGTCATTGATC
TGGCACTTAATACTCAGGCGTCAAATTCGACGTCTCCCGCTGCCGCGTTG
TGGCATTTCTATCAAAACAAAACCAAATTAAAAACGATAATCATGGTAAC
CGATGAAGAAGAAAATACAAACTATCAAGACAATGATTTTATGACTCTAT
TTAAAAAATATATCGACGAAGTCGGGCCTTGTAAATTGATTTTCATTTCC
TTTCTCTCGAGTCAGCATTCTCAAGGACAAATGGTGAATGCGCTTCGGGC
TGCTAACATCGACACTACGCAATACGTTTTTAATTCAATTAAACCCGACA
TTAGTAAATTGGATTCGATTATTGGGAAAATGTCGTTGAGTAATTCCAGG
TTTCTTGACGGATTGGTAGCAATTGAGACTTCATTAGGAAATGAGACTGT
TGAAACGGTTTTTGGAAATATCGGACAATAAGATTTTTTTTAATTTAATA
GTGTTTTTCAGTTTGTTTTTTTAGATAAGTAAAAGGATTTATAATTTTTT
GAAAAAAA
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protein sequence of SMED30030897-orf-1

>SMED30030897-orf-1 ID=SMED30030897-orf-1|Name=SMED30030897-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=559bp
MAPRKLKFHSSKTRRKPIQKLSSAIDNEEILKEYDFLSDPTSYFLNSTSF
DAHLEAQNTYRNRYLATVLQLVNMKIEILPSAKVKAITCAWARKDQEMAN
VFLSMKKIFNFVEVAKAVNLLDIPRQIKATENKINKLKMLEENKKITVKL
STYKTLAKKISTLSKQKLNFGSVSGALCRQVRNWASKFNETELEFFLMNF
DTTVWKEMADIVHFNPQKNFSSLSWFLPYCFGKSAPEISTVERTKNLNSE
NVNEILKSTPLPFHKISKFKQNLTDESKVIIVKNEDNVGRAIWWYEDLEC
CQVDEIIMERLCSNEKLEFGYGKLMERLMMLKPKNVCFYPKLMEIAESKL
SEYSHCLEGPIGVLADASSSMDVAIRTATIISSFLTVFTNAQLIFFNDNY
WIPEKNPTSISDVIDLALNTQASNSTSPAAALWHFYQNKTKLKTIIMVTD
EEENTNYQDNDFMTLFKKYIDEVGPCKLIFISFLSSQHSQGQMVNALRAA
NIDTTQYVFNSIKPDISKLDSIIGKMSLSNSRFLDGLVAIETSLGNETVE
TVFGNIGQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036465vWFA_dom_sf
IPR002035VWF_A
Vocabulary: molecular function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
Vocabulary: biological process
TermDefinition
GO:0016567protein ubiquitination
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCDDcd00198vWFAcoord: 361..459
e-value: 0.00257579
score: 36.775
IPR036465von Willebrand factor A-like domain superfamilySUPERFAMILYSSF53300vWA-likecoord: 358..492