GCM domain-containing protein

Overview


Name	GCM domain-containing protein
Smed ID	SMED30030866
Length (bp)	1600

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of GCM domain-containing protein (SMED30030866) t-SNE clustered cells

Violin plots show distribution of expression levels for GCM domain-containing protein (SMED30030866) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCM domain-containing protein (SMED30030866) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCM domain-containing protein (SMED30030866) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30030866

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
cephalic ganglia	SMED30030866	SMESG000056241.1	dd_Smed_v4_7752_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30030866	SMESG000056241.1	dd_Smed_v4_7752_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30030866	SMESG000056241.1	dd_Smed_v4_7752_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30030866	SMESG000056241.1	dd_Smed_v4_7752_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30030866	SMESG000056241.1	Contig49468	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30030866	SMESG000056241.1	Contig49468	uc_Smed_v2	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of GCM domain-containing protein vs. Ensembl Human
Match: GCM2 (glial cells missing transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:4198])

HSP 1 Score: 135.961 bits (341), Expect = 7.311e-34
Identity = 83/212 (39.15%), Postives = 123/212 (58.02%), Query Frame = 2

Query:  494 WDIKDAVLPQP-EFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR--LKCEKLNFNELNEFLFHDENFDQ-KFNSVKYEHLPRI-NPENIFNIPLECMY 1114
            WDI D  +PQ    +  F  WPDG    IY++   KA++H SGWAMRNTNNHN  ILKKSCLGV+ C++ C  P+  S ++L P I + AR  Q  KK CPN  C   L    C G SG PVT++WR +   N+I+F A+G HDH +P  + +   +R  +K +  +F +  +    +   ++ + +S  + ++P + NPE+ F+I  E  +
Sbjct:   21 WDINDPQMPQELALFDQFREWPDGYVRFIYSSDEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCTQACTLPD-GSRLQLRPAICDKARLKQ-QKKACPN--CHSALELIPCRGHSGYPVTNFWRLD--GNAIFFQAKGVHDHPRPESKSETEARRSAIKRQMASFYQPQKKRIRESEAEENQDSSGHFSNIPPLENPED-FDIVTETSF 225

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Human
Match: GCM1 (glial cells missing transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:4197])

HSP 1 Score: 124.79 bits (312), Expect = 2.406e-30
Identity = 69/161 (42.86%), Postives = 94/161 (58.39%), Query Frame = 2

Query:  485 ISDWDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            I  WDI D  LPQ  +    F+ WPD     IY++ +  A++H S WAMRNTNNHN  ILKKSCLGV+ C + C    +   + L P I + AR+ Q  K+ CPN  CDG L    C G  G PVT++WR++     I+F ++G HDH KP  +++   +R
Sbjct:   13 ILSWDINDVKLPQNVKKTDWFQEWPDSYAKHIYSSEDKNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCGRDC-LAEEGRKIYLRPAICDKARQKQQRKR-CPN--CDGPLKLIPCRGHGGFPVTNFWRHDGRF--IFFQSKGEHDHPKPETKLEAEARR 167

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Fly
Match: gcm (gene:FBgn0014179 transcript:FBtr0335492)

HSP 1 Score: 165.622 bits (418), Expect = 9.568e-45
Identity = 85/187 (45.45%), Postives = 109/187 (58.29%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+ +P    +  F  W +G C LIY+  +++ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN  S V L P I + ARR Q  K+ CPN +C+G L  Q C G  G PVTH+WR  +  N IYF A+G+HDH +P  +     +RL         L   L  +     K +S++
Sbjct:   33 DWDINDSKMPSVGEFDDFNDWSNGHCRLIYSVQSDEARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQ-CPNRNCNGRLEIQACRGHCGYPVTHFWR--RDGNGIYFQAKGTHDHPRPEAKGSTEARRLLAGGRRVRSLAVMLARESALSDKLSSLR 215

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Fly
Match: gcm (gene:FBgn0014179 transcript:FBtr0079855)

HSP 1 Score: 165.622 bits (418), Expect = 9.568e-45
Identity = 85/187 (45.45%), Postives = 109/187 (58.29%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+ +P    +  F  W +G C LIY+  +++ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN  S V L P I + ARR Q  K+ CPN +C+G L  Q C G  G PVTH+WR  +  N IYF A+G+HDH +P  +     +RL         L   L  +     K +S++
Sbjct:   33 DWDINDSKMPSVGEFDDFNDWSNGHCRLIYSVQSDEARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQ-CPNRNCNGRLEIQACRGHCGYPVTHFWR--RDGNGIYFQAKGTHDHPRPEAKGSTEARRLLAGGRRVRSLAVMLARESALSDKLSSLR 215

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Fly
Match: gcm2 (gene:FBgn0019809 transcript:FBtr0079837)

HSP 1 Score: 143.28 bits (360), Expect = 2.632e-36
Identity = 76/161 (47.20%), Postives = 101/161 (62.73%), Query Frame = 2

Query:  491 DWDIKDAVLPQ--PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQ-QLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            +WDI DA++P    + +  F  W DG    IY+  N +A+KH SGWAMRNTNNHN  ILKKSCLGV+ CS+ C  PN  S + L P I + ARR Q  K  CPN  C G  ++ + C G  G PVTH+WR++   N+I+F A+G HDH +P+ +   + KR
Sbjct:   66 EWDINDAIVPHVPDQEFDEFNEWSDGHVRHIYSLHNEEAKKHISGWAMRNTNNHNVNILKKSCLGVLVCSQHCTLPN-GSKINLRPAICDKARRKQEGKA-CPNKSCRGGRLEIKPCRGHCGYPVTHFWRHS--GNAIFFQAKGVHDHLRPDPKNSSVSKR 222

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BLAST of GCM domain-containing protein vs. Ensembl Zebrafish
Match: gcm2 (glial cells missing transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-050127-1])

HSP 1 Score: 132.494 bits (332), Expect = 5.501e-33
Identity = 73/163 (44.79%), Postives = 101/163 (61.96%), Query Frame = 2

Query:  494 WDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEK 979
            WDI D  LPQ  + Y +F+ W DG    IY++ +  A++H SGWAMRNTNNHN  ILKKSCLGV+ CS+ C  P+  S ++L P I + AR+ Q  KK CPN  C+  L    C G SG PVT++WR +    +I+F A+G HDH +P  + +   +R   ++
Sbjct:    5 WDINDPKLPQDTKQYDAFQEWTDGYVRYIYSSEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCSRNCSLPD-GSKLQLRPAICDKARQKQ-QKKLCPN--CNSALELIPCRGHSGYPVTNFWRVD--GKAIFFQAKGVHDHPRPESKSETEARRSAVKR 161

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Zebrafish
Match: gcm2 (glial cells missing transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-050127-1])

HSP 1 Score: 132.494 bits (332), Expect = 6.394e-33
Identity = 73/163 (44.79%), Postives = 101/163 (61.96%), Query Frame = 2

Query:  494 WDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEK 979
            WDI D  LPQ  + Y +F+ W DG    IY++ +  A++H SGWAMRNTNNHN  ILKKSCLGV+ CS+ C  P+  S ++L P I + AR+ Q  KK CPN  C+  L    C G SG PVT++WR +    +I+F A+G HDH +P  + +   +R   ++
Sbjct:   23 WDINDPKLPQDTKQYDAFQEWTDGYVRYIYSSEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCSRNCSLPD-GSKLQLRPAICDKARQKQ-QKKLCPN--CNSALELIPCRGHSGYPVTNFWRVD--GKAIFFQAKGVHDHPRPESKSETEARRSAVKR 179

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Xenopus
Match: gcm1 (glial cells missing transcription factor 1 [Source:NCBI gene;Acc:549669])

HSP 1 Score: 137.502 bits (345), Expect = 4.090e-35
Identity = 84/207 (40.58%), Postives = 112/207 (54.11%), Query Frame = 2

Query:  488 SDWDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLE 1105
            S+WDI D  LPQ PE    F+ WPD     IY+++N  A++H SGWAMRNTNNHN  ILKKSCLGV+ CS  C  P+    V L P I + AR+ Q +K  CPN  C G L    C G  G PVT++WR+      IYF  +G HDH KP  +++   +++  +K N     +        D+     K +       EN+ N PL+
Sbjct:   22 SNWDINDMKLPQDPEQTDWFQEWPDSYVKHIYSSSNRNAQRHLSGWAMRNTNNHNSRILKKSCLGVLVCSNDCTVPDGRK-VYLRPAICDKARQKQQSKH-CPN--CSGPLKLISCRGHGGFPVTNFWRH--EGPYIYFQTKGVHDHPKPETKLESESRKVGHKKRNAIVTTKLGLKRSRNDEALTGEKADQ------ENLSNTPLD 216

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Xenopus
Match: GCM2 (glial cells missing homolog 2 [Source:NCBI gene;Acc:100488293])

HSP 1 Score: 128.257 bits (321), Expect = 2.558e-31
Identity = 72/163 (44.17%), Postives = 98/163 (60.12%), Query Frame = 2

Query:  494 WDIKDAVLPQP-EFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEK 979
            WDI D  LPQ  + + SF+ W DG    IYNA +  A++H SGWAMRNTNNHN  ILKKSCLGV+ CS+ C   +   ++ L P I + AR+ Q  KK C N  C+  L    C G SG PVT++WR +    +I+F A+G HDH +P  + +   +R   ++
Sbjct:   24 WDINDPKLPQDLKQFDSFQEWTDGYVRFIYNAEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCSRNCTLLDGGKLL-LRPAICDKARQKQ-QKKMCSN--CNSALELIPCRGHSGYPVTNFWRLD--GKAIFFQAKGVHDHPRPESKSETEARRSAVKR 180

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Xenopus
Match: gcm1 (glial cells missing transcription factor 1 [Source:NCBI gene;Acc:549669])

HSP 1 Score: 125.946 bits (315), Expect = 3.113e-31
Identity = 77/199 (38.69%), Postives = 105/199 (52.76%), Query Frame = 2

Query:  509 AVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLE 1105
            A +  PE    F+ WPD     IY+++N  A++H SGWAMRNTNNHN  ILKKSCLGV+ CS  C  P+    V L P I + AR+ Q +K  CPN  C G L    C G  G PVT++WR+      IYF  +G HDH KP  +++   +++  +K N     +        D+     K +       EN+ N PL+
Sbjct:    9 AYILDPEQTDWFQEWPDSYVKHIYSSSNRNAQRHLSGWAMRNTNNHNSRILKKSCLGVLVCSNDCTVPDGRK-VYLRPAICDKARQKQQSKH-CPN--CSGPLKLISCRGHGGFPVTNFWRH--EGPYIYFQTKGVHDHPKPETKLESESRKVGHKKRNAIVTTKLGLKRSRNDEALTGEKADQ------ENLSNTPLD 195

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Mouse
Match: Gcm2 (glial cells missing homolog 2 [Source:MGI Symbol;Acc:MGI:1861438])

HSP 1 Score: 133.265 bits (334), Expect = 5.479e-36
Identity = 73/158 (46.20%), Postives = 96/158 (60.76%), Query Frame = 2

Query:  494 WDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            WDI D  +PQ P  +  F  WPDG    IY++   KA++H SGWAMRNTNNHN  ILKKSCLGV+ C++ C   +  S ++L P I + AR  Q  KK CPN  C   L    C G SG PVT++WR +   N+I+F A+G HDH +P  + +   +R
Sbjct:   21 WDINDPQMPQEPTHFDHFREWPDGYVRFIYSSQEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCARACALKD-GSHLQLRPAICDKARLKQ-QKKACPN--CHSPLELVPCRGHSGYPVTNFWRLD--GNAIFFQAKGVHDHPRPESKSETEGRR 172

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Mouse
Match: Gcm2 (glial cells missing homolog 2 [Source:MGI Symbol;Acc:MGI:1861438])

HSP 1 Score: 135.961 bits (341), Expect = 4.855e-34
Identity = 76/168 (45.24%), Postives = 100/168 (59.52%), Query Frame = 2

Query:  494 WDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR--LKCEKLNF 988
            WDI D  +PQ P  +  F  WPDG    IY++   KA++H SGWAMRNTNNHN  ILKKSCLGV+ C++ C      S ++L P I + AR  Q  KK CPN  C   L    C G SG PVT++WR +   N+I+F A+G HDH +P  + +   +R  LK +  +F
Sbjct:   21 WDINDPQMPQEPTHFDHFREWPDGYVRFIYSSQEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCARACAL-KDGSHLQLRPAICDKARLKQ-QKKACPN--CHSPLELVPCRGHSGYPVTNFWRLD--GNAIFFQAKGVHDHPRPESKSETEGRRSALKRQMASF 182

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Mouse
Match: Gcm1 (glial cells missing homolog 1 [Source:MGI Symbol;Acc:MGI:108045])

HSP 1 Score: 122.865 bits (307), Expect = 6.664e-30
Identity = 68/161 (42.24%), Postives = 95/161 (59.01%), Query Frame = 2

Query:  485 ISDWDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            I  WDI D  LPQ  +    F+ WPD     IY++ +  A++H S WAMRNTNNHN  ILKKSCLGV+ CS+ C    +   + L P I + AR+ Q  +K CPN  C+G L    C G  G PVT++WR++     I+F ++G HDH +P  +++   +R
Sbjct:   13 ILSWDINDVKLPQNVKTTDWFQEWPDSYVKHIYSSDDRNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCSRDC-STEEGRKIYLRPAICDKARQKQ-QRKSCPN--CNGPLKLIPCRGHGGFPVTNFWRHDGRF--IFFQSKGEHDHPRPETKLEAEARR 167

[Close]

BLAST of GCM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q27403|GCM_DROME (Transcription factor glial cells missing OS=Drosophila melanogaster OX=7227 GN=gcm PE=1 SV=2)

HSP 1 Score: 165.622 bits (418), Expect = 9.591e-44
Identity = 85/187 (45.45%), Postives = 109/187 (58.29%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+ +P    +  F  W +G C LIY+  +++ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN  S V L P I + ARR Q  K+ CPN +C+G L  Q C G  G PVTH+WR  +  N IYF A+G+HDH +P  +     +RL         L   L  +     K +S++
Sbjct:   33 DWDINDSKMPSVGEFDDFNDWSNGHCRLIYSVQSDEARKHASGWAMRNTNNHNVNILKKSCLGVLLCSAKCKLPNGAS-VHLRPAICDKARRKQQGKQ-CPNRNCNGRLEIQACRGHCGYPVTHFWR--RDGNGIYFQAKGTHDHPRPEAKGSTEARRLLAGGRRVRSLAVMLARESALSDKLSSLR 215

[Close]

BLAST of GCM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9VLA2|GCM2_DROME (Transcription factor glial cells missing 2 OS=Drosophila melanogaster OX=7227 GN=gcm2 PE=2 SV=4)

HSP 1 Score: 143.28 bits (360), Expect = 2.638e-35
Identity = 76/161 (47.20%), Postives = 101/161 (62.73%), Query Frame = 2

Query:  491 DWDIKDAVLPQ--PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQ-QLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            +WDI DA++P    + +  F  W DG    IY+  N +A+KH SGWAMRNTNNHN  ILKKSCLGV+ CS+ C  PN  S + L P I + ARR Q  K  CPN  C G  ++ + C G  G PVTH+WR++   N+I+F A+G HDH +P+ +   + KR
Sbjct:   66 EWDINDAIVPHVPDQEFDEFNEWSDGHVRHIYSLHNEEAKKHISGWAMRNTNNHNVNILKKSCLGVLVCSQHCTLPN-GSKINLRPAICDKARRKQEGKA-CPNKSCRGGRLEIKPCRGHCGYPVTHFWRHS--GNAIFFQAKGVHDHLRPDPKNSSVSKR 222

[Close]

BLAST of GCM domain-containing protein vs. UniProt/SwissProt
Match: sp|O09102|GCM2_MOUSE (Chorion-specific transcription factor GCMb OS=Mus musculus OX=10090 GN=Gcm2 PE=2 SV=2)

HSP 1 Score: 135.961 bits (341), Expect = 3.205e-33
Identity = 76/168 (45.24%), Postives = 100/168 (59.52%), Query Frame = 2

Query:  494 WDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR--LKCEKLNF 988
            WDI D  +PQ P  +  F  WPDG    IY++   KA++H SGWAMRNTNNHN  ILKKSCLGV+ C++ C      S ++L P I + AR  Q  KK CPN  C   L    C G SG PVT++WR +   N+I+F A+G HDH +P  + +   +R  LK +  +F
Sbjct:   21 WDINDPQMPQEPTHFDHFREWPDGYVRFIYSSQEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCARACAL-KDGSHLQLRPAICDKARLKQ-QKKACPN--CHSPLELVPCRGHSGYPVTNFWRLD--GNAIFFQAKGVHDHPRPESKSETEGRRSALKRQMASF 182

[Close]

BLAST of GCM domain-containing protein vs. UniProt/SwissProt
Match: sp|O75603|GCM2_HUMAN (Chorion-specific transcription factor GCMb OS=Homo sapiens OX=9606 GN=GCM2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 3.511e-33
Identity = 83/212 (39.15%), Postives = 123/212 (58.02%), Query Frame = 2

Query:  494 WDIKDAVLPQP-EFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR--LKCEKLNFNELNEFLFHDENFDQ-KFNSVKYEHLPRI-NPENIFNIPLECMY 1114
            WDI D  +PQ    +  F  WPDG    IY++   KA++H SGWAMRNTNNHN  ILKKSCLGV+ C++ C  P+  S ++L P I + AR  Q  KK CPN  C   L    C G SG PVT++WR +   N+I+F A+G HDH +P  + +   +R  +K +  +F +  +    +   ++ + +S  + ++P + NPE+ F+I  E  +
Sbjct:   21 WDINDPQMPQELALFDQFREWPDGYVRFIYSSDEKKAQRHLSGWAMRNTNNHNGHILKKSCLGVVVCTQACTLPD-GSRLQLRPAICDKARLKQ-QKKACPN--CHSALELIPCRGHSGYPVTNFWRLD--GNAIFFQAKGVHDHPRPESKSETEARRSAIKRQMASFYQPQKKRIRESEAEENQDSSGHFSNIPPLENPED-FDIVTETSF 225

[Close]

BLAST of GCM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9NP62|GCM1_HUMAN (Chorion-specific transcription factor GCMa OS=Homo sapiens OX=9606 GN=GCM1 PE=1 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.155e-29
Identity = 69/161 (42.86%), Postives = 94/161 (58.39%), Query Frame = 2

Query:  485 ISDWDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            I  WDI D  LPQ  +    F+ WPD     IY++ +  A++H S WAMRNTNNHN  ILKKSCLGV+ C + C    +   + L P I + AR+ Q  K+ CPN  CDG L    C G  G PVT++WR++     I+F ++G HDH KP  +++   +R
Sbjct:   13 ILSWDINDVKLPQNVKKTDWFQEWPDSYAKHIYSSEDKNAQRHLSSWAMRNTNNHNSRILKKSCLGVVVCGRDC-LAEEGRKIYLRPAICDKARQKQQRKR-CPN--CDGPLKLIPCRGHGGFPVTNFWRHDGRF--IFFQSKGEHDHPKPETKLEAEARR 167

[Close]

BLAST of GCM domain-containing protein vs. TrEMBL
Match: A0A1B0FMT7 (GCM domain-containing protein OS=Glossina morsitans morsitans OX=37546 PE=4 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 9.699e-46
Identity = 92/187 (49.20%), Postives = 113/187 (60.43%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+V+P    Y  F+ W +G C L+Y+A N  ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN N+ V L P I + ARR Q   KPCPN +C G L  Q C G  G PVTH+WR  +  NSI+F A+G+HDH +P  +     +RL         L   L  D   ++K NS+K
Sbjct:   26 DWDINDSVVPHVTEYDDFQDWANGHCRLVYSANNEDARKHSSGWAMRNTNNHNINILKKSCLGVLLCSDKCKLPNGNN-VNLRPAICDKARRKQ-QGKPCPNRNCSGRLEIQPCRGHCGYPVTHFWR--RSGNSIFFQAKGTHDHPRPEAKGSSEARRLLGTGRRVRSLAVLLARDAALNEKLNSLK 208

[Close]

BLAST of GCM domain-containing protein vs. TrEMBL
Match: A0A1A9XGX9 (GCM domain-containing protein OS=Glossina fuscipes fuscipes OX=201502 PE=4 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.070e-45
Identity = 92/187 (49.20%), Postives = 113/187 (60.43%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+V+P    Y  F+ W +G C L+Y+A N  ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN N+ V L P I + ARR Q   KPCPN +C G L  Q C G  G PVTH+WR  +  NSI+F A+G+HDH +P  +     +RL         L   L  D   ++K NS+K
Sbjct:   26 DWDINDSVVPHVTEYDDFQDWANGHCRLVYSANNEDARKHSSGWAMRNTNNHNINILKKSCLGVLLCSDKCKLPNGNN-VNLRPAICDKARRKQ-QGKPCPNRNCSGRLEIQPCRGHCGYPVTHFWR--RSGNSIFFQAKGTHDHPRPEAKGSSEARRLLGTGRRVRSLAVLLARDAALNEKLNSLK 208

[Close]

BLAST of GCM domain-containing protein vs. TrEMBL
Match: A0A1B0A4X9 (GCM domain-containing protein OS=Glossina pallidipes OX=7398 PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 1.249e-45
Identity = 92/187 (49.20%), Postives = 113/187 (60.43%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+V+P    Y  F+ W +G C L+Y+A N  ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN N+ V L P I + ARR Q   KPCPN +C G L  Q C G  G PVTH+WR  +  NSI+F A+G+HDH +P  +     +RL         L   L  D   ++K NS+K
Sbjct:   26 DWDINDSVVPHVTEYDDFQDWANGHCRLVYSANNEDARKHSSGWAMRNTNNHNINILKKSCLGVLLCSDKCKLPNGNN-VNLRPAICDKARRKQ-QGKPCPNRNCSGRLEIQPCRGHCGYPVTHFWR--RSGNSIFFQAKGTHDHPRPEAKGSSEARRLLGTGRRVRSLAVLLARDAALNEKLNSLK 208

[Close]

BLAST of GCM domain-containing protein vs. TrEMBL
Match: A0A1A9W012 (GCM domain-containing protein OS=Glossina brevipalpis OX=37001 PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 1.697e-45
Identity = 92/187 (49.20%), Postives = 114/187 (60.96%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+V+P    Y  F+ W +G C L+Y+A+N  ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN N+ V L P I + ARR Q   KPCPN +C G L  + C G  G PVTH+WR  +  NSI+F A+G+HDH KP  +     +RL         L   L  D   ++K NS+K
Sbjct:   26 DWDINDSVVPNVTEYDEFQDWANGHCRLVYSASNEDARKHSSGWAMRNTNNHNINILKKSCLGVLLCSDKCKLPNGNN-VNLRPAICDKARRKQ-QGKPCPNRNCSGRLEIRPCRGHCGYPVTHFWR--RSGNSIFFQAKGTHDHPKPEAKGSSEARRLLGTGRRVRSLAVLLARDAALNEKLNSLK 208

[Close]

BLAST of GCM domain-containing protein vs. TrEMBL
Match: A0A1A9V9N8 (GCM domain-containing protein OS=Glossina austeni OX=7395 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 5.415e-45
Identity = 92/187 (49.20%), Postives = 113/187 (60.43%), Query Frame = 2

Query:  491 DWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVK 1051
            DWDI D+V+P    Y  F+ W +G C L+Y+A N  ARKH SGWAMRNTNNHN  ILKKSCLGV+ CS  C  PN N+ V L P I + ARR Q   KPCPN +C G L  Q C G  G PVTH+WR  +  NSI+F A+G+HDH +P  +     +RL         L   L  D   ++K NS+K
Sbjct:  103 DWDINDSVVPHVTEYDDFQDWANGHCRLVYSANNEDARKHSSGWAMRNTNNHNINILKKSCLGVLLCSDKCKLPNGNN-VNLRPAICDKARRKQ-QGKPCPNRNCSGRLEIQPCRGHCGYPVTHFWR--RSDNSIFFQAKGTHDHPRPEAKGSSEARRLLGTGRRVRSLAVLLARDAALNEKLNSLK 285

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Cavefish
Match: gcm2 (glial cells missing transcription factor 2 [Source:NCBI gene;Acc:103026873])

HSP 1 Score: 130.568 bits (327), Expect = 2.868e-32
Identity = 71/163 (43.56%), Postives = 99/163 (60.74%), Query Frame = 2

Query:  494 WDIKDAVLPQ-PEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEK 979
            WDI D  LPQ P+ +  F+ W DG    IY+A +  A++H SGWAMRNTNNHN  ILKKSCLGV+ C++ C   +  + ++L P I + AR+ Q  KK CPN  C   L    C G SG PVT++WR +    +I+F A+G HDH +P  + +   +R   ++
Sbjct:   23 WDINDPKLPQDPKQFDPFQEWTDGYVRYIYSAEDKNAQRHLSGWAMRNTNNHNCQILKKSCLGVVVCARGCTLAD-GTKLQLRPAICDKARQKQ-QKKLCPN--CSSALELVPCRGHSGYPVTNFWRVD--GKAIFFQAKGVHDHPRPESKSETEARRSAVKR 179

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Sea Lamprey
Match: gcm2 (glial cells missing transcription factor 2 [Source:ZFIN;Acc:ZDB-GENE-050127-1])

HSP 1 Score: 126.716 bits (317), Expect = 1.164e-31
Identity = 71/163 (43.56%), Postives = 95/163 (58.28%), Query Frame = 2

Query:  494 WDIKDAVLPQP-EFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEK 979
            WDI D  LPQ  +    F+ W DG    +++A +  A++H SGWAMRNTNNHN  ILKKSCLGV+ C + C  P+    V L P I + AR+ Q  KK CPN  C   L    C G SG PVT++WR+      I+F A+GSHDH +P  + +   +R   ++
Sbjct:   23 WDINDPKLPQDLKQVDPFQEWNDGYARFVFSAEDKNAQRHLSGWAMRNTNNHNCAILKKSCLGVVACGRACTMPDGRK-VHLRPAICDKARQKQ-QKKLCPN--CGSPLDLLPCRGHSGYPVTNFWRHEGRF--IFFQAKGSHDHPRPESKTEAEARRSSSKR 179

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001147.1 (pep scaffold:Pmarinus_7.0:GL476699:2797:4762:1 gene:ENSPMAG00000001027.1 transcript:ENSPMAT00000001147.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 5.568e-8
Identity = 25/66 (37.88%), Postives = 36/66 (54.55%), Query Frame = 2

Query:  782 KPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEK 979
            K CPN  C G L    C G SG PVT++WR+      ++F A+G HDH +P  + +   +R   +K
Sbjct:    1 KMCPN--CQGPLELVPCRGHSGYPVTNFWRHEGKL--VFFQAKGVHDHPRPESKTEAEGRRCAAKK 62

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Nematostella
Match: EDO33215 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSU8])

HSP 1 Score: 129.413 bits (324), Expect = 2.269e-35
Identity = 64/143 (44.76%), Postives = 89/143 (62.24%), Query Frame = 2

Query:  533 YHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDK 961
            +  F  W DG C L Y+A + +A+ H SGWAM+ TNNHNK +LKK+C+GV+ CSK C  PN   IV + P IS+  R  Q+ +  CPN  C G L  + CTG +G PVTH+W +    + IYF ++G+HDH +P  +    D+
Sbjct:   11 FDEFNEWIDGSCKLRYSAYSREAQAHISGWAMKYTNNHNKYVLKKTCVGVLLCSKDCTLPNGLKIV-VRPAISDKVRERQIGQN-CPNASCSGILSHRKCTGNNGYPVTHFWVHQD--DGIYFESKGTHDHFRPQARRATPDR 149

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Nematostella
Match: EDO25565 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DW44])

HSP 1 Score: 128.642 bits (322), Expect = 2.735e-35
Identity = 63/134 (47.01%), Postives = 86/134 (64.18%), Query Frame = 2

Query:  533 YHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKP 934
            +  F  W DG C L Y+A + +A+ H SGWAM+ TNNHNK +LKK+C+GV+ CSK C  PN   IV + P IS+  R  Q+ +  CPN  C G L  + CTG +G PVTH+W +    + IYF ++G+HDH +P
Sbjct:   11 FDEFNEWIDGSCKLRYSAYSREAQAHISGWAMKYTNNHNKYVLKKTCVGVLLCSKDCTLPNGLKIV-VRPAISDKVRERQIGQN-CPNASCSGILSHRKCTGNNGYPVTHFWVHQD--DGIYFESKGTHDHFRP 140

[Close]

BLAST of GCM domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000033677.1 (glial cells missing transcription factor 2 [Source:NCBI gene;Acc:101156002])

HSP 1 Score: 92.4337 bits (228), Expect = 6.938e-20
Identity = 53/113 (46.90%), Postives = 70/113 (61.95%), Query Frame = 2

Query:  626 MRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKR 964
            MRNTNNHN  ILKKSCLGV+ CS+ C  P+  S ++L P I + AR+ Q  KK CP+  C   L    C G SG PVT++WR +    SI+F A+G HDH +P  + +   +R
Sbjct:    1 MRNTNNHNCQILKKSCLGVVVCSRGCSLPD-GSRLQLRPAICDKARQKQ-QKKLCPS--CSAGLELLPCRGHSGYPVTNFWRVD--GKSIFFQAKGVHDHPRPESKSETEARR 107

[Close]

BLAST of GCM domain-containing protein vs. Planmine SMEST
Match: SMESG000056241.1 (SMESG000056241.1)

HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0
Identity = 411/438 (93.84%), Postives = 414/438 (94.52%), Query Frame = 2

Query:  257 MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLXXXXXXXXXXXEFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIKPHVEIFPSDINRNCQYISPVYXXXXXXXXXXXXXXXXHMSPLKNSSDFVNDEIQRLYCPEFQQNPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKESLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK 1570
            MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLSTNNNNNNSNTEFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHK                      VIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIKPHVEIFPSD+NRNCQYISPVYNSS+SNSTDSINDNWNHMSPLK+SSDFVNDEIQRLYCPEF  NPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKESLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK
Sbjct:    1 MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLSTNNNNNNSNTEFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKR---------------------VIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIKPHVEIFPSDLNRNCQYISPVYNSSVSNSTDSINDNWNHMSPLKHSSDFVNDEIQRLYCPEFHMNPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKESLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK 417

[Close]

BLAST of GCM domain-containing protein vs. Planmine SMEST
Match: SMESG000056241.1 (SMESG000056241.1)

HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0
Identity = 392/438 (89.50%), Postives = 396/438 (90.41%), Query Frame = 2

Query:  257 MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLXXXXXXXXXXXEFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWPDGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIKPHVEIFPSDINRNCQYISPVYXXXXXXXXXXXXXXXXHMSPLKNSSDFVNDEIQRLYCPEFQQNPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKESLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK 1570
            MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLSTNNNNNNSNTEFHEHNSADAG    CFA              +FYHSFEMWPDGDCHLIYNATNNKARKHK                      VIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIKPHVEIFPSD+NRNCQYISPVYNSS+SNSTDSINDNWNHMSPLK+SSDFVNDEIQRLYCPEF  NPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKESLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK
Sbjct:    1 MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLSTNNNNNNSNTEFHEHNSADAGC---CFAT-------------KFYHSFEMWPDGDCHLIYNATNNKARKHKR---------------------VIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIKPHVEIFPSDLNRNCQYISPVYNSSVSNSTDSINDNWNHMSPLKHSSDFVNDEIQRLYCPEFHMNPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKESLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK 401

[Close]

BLAST of GCM domain-containing protein vs. Planmine SMEST
Match: SMESG000036444.1 (SMESG000036444.1)

HSP 1 Score: 159.458 bits (402), Expect = 1.121e-43
Identity = 84/196 (42.86%), Postives = 117/196 (59.69%), Query Frame = 2

Query:  431 EFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWPDGDCHLIYN-ATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCWYPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPVTHYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEFLFH 1015
              H  +S +  +  +   I++WD+ D  LPQ    + F++WPDG   L+Y+ + N + R+H SGWAMRNTNNHN+ ILKKSCLGV+ C K C  P     V L P I + AR+ Q+  K CPN  C+G LI Q C G  G PVTHYWR ++  + ++F A+G HDH KP+++    +++   E  N    N FL H
Sbjct:   11 RMHLESSINIDVTATTPLINEWDVADLSLPQLIETNDFQIWPDGHIKLVYDYSKNERVRRHISGWAMRNTNNHNREILKKSCLGVLLCEKKCIRPGTREYVVLRPAICDKARKKQMKNK-CPN--CNGNLILQPCKGNLGFPVTHYWRRDE--DRVFFQAKGIHDHLKPDLRPHRENRKKSSESNNVYMENNFLIH 201

The following BLAST results are available for this feature:

BLAST of GCM domain-containing protein vs. Ensembl Human
BLAST of GCM domain-containing protein vs. Ensembl Celegans
BLAST of GCM domain-containing protein vs. Ensembl Fly
BLAST of GCM domain-containing protein vs. Ensembl Zebrafish
BLAST of GCM domain-containing protein vs. Ensembl Xenopus
BLAST of GCM domain-containing protein vs. Ensembl Mouse
BLAST of GCM domain-containing protein vs. UniProt/SwissProt
BLAST of GCM domain-containing protein vs. TrEMBL
BLAST of GCM domain-containing protein vs. Ensembl Cavefish
BLAST of GCM domain-containing protein vs. Ensembl Sea Lamprey
BLAST of GCM domain-containing protein vs. Ensembl Yeast
BLAST of GCM domain-containing protein vs. Ensembl Nematostella
BLAST of GCM domain-containing protein vs. Ensembl Medaka
BLAST of GCM domain-containing protein vs. Planmine SMEST

BLAST of GCM domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
GCM2	7.311e-34	39.15	glial cells missing transcription factor 2 [Source...	[more]
GCM1	2.406e-30	42.86	glial cells missing transcription factor 1 [Source...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of GCM domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
gcm	9.568e-45	45.45	gene:FBgn0014179 transcript:FBtr0335492	[more]
gcm	9.568e-45	45.45	gene:FBgn0014179 transcript:FBtr0079855	[more]
gcm2	2.632e-36	47.20	gene:FBgn0019809 transcript:FBtr0079837	[more]

BLAST of GCM domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gcm2	5.501e-33	44.79	glial cells missing transcription factor 2 [Source...	[more]
gcm2	6.394e-33	44.79	glial cells missing transcription factor 2 [Source...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
gcm1	4.090e-35	40.58	glial cells missing transcription factor 1 [Source...	[more]
GCM2	2.558e-31	44.17	glial cells missing homolog 2 [Source:NCBI gene;Ac...	[more]
gcm1	3.113e-31	38.69	glial cells missing transcription factor 1 [Source...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Gcm2	5.479e-36	46.20	glial cells missing homolog 2 [Source:MGI Symbol;A...	[more]
Gcm2	4.855e-34	45.24	glial cells missing homolog 2 [Source:MGI Symbol;A...	[more]
Gcm1	6.664e-30	42.24	glial cells missing homolog 1 [Source:MGI Symbol;A...	[more]

BLAST of GCM domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q27403\|GCM_DROME	9.591e-44	45.45	Transcription factor glial cells missing OS=Drosop...	[more]
sp\|Q9VLA2\|GCM2_DROME	2.638e-35	47.20	Transcription factor glial cells missing 2 OS=Dros...	[more]
sp\|O09102\|GCM2_MOUSE	3.205e-33	45.24	Chorion-specific transcription factor GCMb OS=Mus ...	[more]
sp\|O75603\|GCM2_HUMAN	3.511e-33	39.15	Chorion-specific transcription factor GCMb OS=Homo...	[more]
sp\|Q9NP62\|GCM1_HUMAN	1.155e-29	42.86	Chorion-specific transcription factor GCMa OS=Homo...	[more]

BLAST of GCM domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1B0FMT7	9.699e-46	49.20	GCM domain-containing protein OS=Glossina morsitan...	[more]
A0A1A9XGX9	1.070e-45	49.20	GCM domain-containing protein OS=Glossina fuscipes...	[more]
A0A1B0A4X9	1.249e-45	49.20	GCM domain-containing protein OS=Glossina pallidip...	[more]
A0A1A9W012	1.697e-45	49.20	GCM domain-containing protein OS=Glossina brevipal...	[more]
A0A1A9V9N8	5.415e-45	49.20	GCM domain-containing protein OS=Glossina austeni ...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gcm2	2.868e-32	43.56	glial cells missing transcription factor 2 [Source...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gcm2	1.164e-31	43.56	glial cells missing transcription factor 2 [Source...	[more]
ENSPMAT00000001147.1	5.568e-8	37.88	pep scaffold:Pmarinus_7.0:GL476699:2797:4762:1 gen...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of GCM domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO33215	2.269e-35	44.76	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO25565	2.735e-35	47.01	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8...	[more]

BLAST of GCM domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSORLT00000033677.1	6.938e-20	46.90	glial cells missing transcription factor 2 [Source...	[more]

BLAST of GCM domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000056241.1	0.000e+0	93.84	SMESG000056241.1	[more]
SMESG000056241.1	0.000e+0	89.50	SMESG000056241.1	[more]
SMESG000036444.1	1.121e-43	42.86	SMESG000036444.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30030866 ID=SMED30030866|Name=GCM domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1600bp
CCTTCAACGGATAAAACTAATGAAAAAATATTGTTGTTTTGTTGATTTTT
GAAATTTAGTAATGAGAATTGAATAGTTTTCATTTATTCCTTCAAATCAG
CTATAGAGAAATTAAAAAAAATTTTAAAAATAATTTAACGAGCGCCAATC
GATTTGCCTGTAAGAGCACTTGCACTGTGGTGCTCAAATCCTATTGGATA
AAATCAATAGTAATAATGGCTCCCGCAAAATAAGATTATATTACATTTCA
CCGCTAATGAATGACCCGTATGACTACATAGATTGTCAAAATTACCCACA
TAATCAATTCAATTATAATTCATTTAAGTCATTTCAAACACAAAATGGAT
GGATATTTGGTGGAGATGGGGATTTGGTGCAAATAAATGACGGCTTAAGT
ACCAATAACAACAATAATAATAGTAATACGGAATTTCACGAGCACAACTC
TGCCGATGCGGGTATCTTAAATTCGTGTTTTGCGATATCAGATTGGGACA
TTAAGGATGCTGTTTTGCCACAACCAGAATTCTATCACTCATTTGAAATG
TGGCCGGATGGAGACTGTCACTTGATTTATAATGCTACAAATAACAAAGC
AAGGAAACACAAAAGTGGGTGGGCAATGAGGAATACAAATAATCATAATA
AAATGATTTTGAAAAAATCTTGTCTAGGAGTAATCAAGTGTTCCAAAACT
TGTTGGTATCCAAACCAGAATAGCATTGTCAGACTTGCTCCCAAAATAAG
TGAAGCAGCTAGACGTTGTCAATTGAACAAAAAACCATGCCCCAATCCAG
ATTGTGATGGATTTCTAATTCAGCAGCTTTGCACTGGGAAATCCGGTAAT
CCAGTCACCCACTACTGGCGATATAATCAGCATACAAATAGCATATATTT
TATGGCTCGTGGATCCCATGACCATGAAAAACCAAATATCCAAATTAAGA
TGATCGATAAGCGTTTGAAGTGTGAAAAATTGAATTTCAATGAATTAAAT
GAGTTTTTATTTCACGATGAAAATTTTGATCAAAAATTCAATTCAGTCAA
ATACGAACATTTGCCAAGGATAAATCCAGAAAATATTTTCAATATTCCAC
TTGAGTGCATGTACTCTCCGTACGTCAATAAATCCTATCAACAATTAATG
ATAAAGCCTCACGTGGAAATCTTTCCGTCAGATATCAACCGAAACTGTCA
GTATATTTCACCAGTTTATAATTCATCAATATCAAACAGTACTGATTCAA
TTAATGACAACTGGAATCATATGAGTCCTTTGAAGAATAGCTCTGATTTT
GTAAACGACGAAATTCAAAGACTTTACTGTCCTGAATTCCAACAGAATCC
TAACAATGCTAACAATATGGAATATATCTCAAGAAATATCCCATTCGAAA
TATCGTGGAATAACACGTGTGCACCACAGGAGCAAAAAGTAGAAGTTGAT
AAAGAATCTTTATTAATTTGTGAAGAACTTGGTGATTCTATAAATAAGAA
TCAGATAGATTGTACTAATTTCAAATATTCGTTTAGAAATGAATTGGAAA
TGTTGGATTGTTTTACTAAATAAATAGTTTTATTTTTTTGTTTAAAAAAA

protein sequence of SMED30030866-orf-1

>SMED30030866-orf-1 ID=SMED30030866-orf-1|Name=SMED30030866-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=439bp
MNDPYDYIDCQNYPHNQFNYNSFKSFQTQNGWIFGGDGDLVQINDGLSTN
NNNNNSNTEFHEHNSADAGILNSCFAISDWDIKDAVLPQPEFYHSFEMWP
DGDCHLIYNATNNKARKHKSGWAMRNTNNHNKMILKKSCLGVIKCSKTCW
YPNQNSIVRLAPKISEAARRCQLNKKPCPNPDCDGFLIQQLCTGKSGNPV
THYWRYNQHTNSIYFMARGSHDHEKPNIQIKMIDKRLKCEKLNFNELNEF
LFHDENFDQKFNSVKYEHLPRINPENIFNIPLECMYSPYVNKSYQQLMIK
PHVEIFPSDINRNCQYISPVYNSSISNSTDSINDNWNHMSPLKNSSDFVN
DEIQRLYCPEFQQNPNNANNMEYISRNIPFEISWNNTCAPQEQKVEVDKE
SLLICEELGDSINKNQIDCTNFKYSFRNELEMLDCFTK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0001228	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003677	DNA binding

Vocabulary: **biological process**
Term	Definition
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0006355	regulation of transcription, DNA-templated
GO:0006351	transcription, DNA-templated
GO:0007275	multicellular organism development

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0003117	parapharyngeal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR043020	GCM_large
IPR043021	GCM_small
IPR039791	GCM
IPR036115	GCM_dom_sf
IPR003902	Tscrpt_reg_GCM

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043020	GCM, large domain	GENE3D	G3DSA:3.30.1370.90		coord: 107..226 e-value: 2.0E-43 score: 149.1
IPR003902	Transcription regulator GCM domain	PFAM	PF03615	GCM	coord: 94..231 e-value: 1.5E-50 score: 170.7
IPR003902	Transcription regulator GCM domain	PROSITE	PS50807	GCM	coord: 78..239 score: 42.678
IPR043021	GCM, small domain	GENE3D	G3DSA:2.20.28.80		coord: 142..230 e-value: 2.0E-43 score: 149.1
None	No IPR available	PANTHER	PTHR12414:SF8	TRANSCRIPTION FACTOR GLIAL CELLS MISSING-RELATED	coord: 78..228
None	No IPR available	PRODOM	PD014393		coord: 79..265 e-value: 2.0E-39 score: 410.0
IPR039791	Chorion-specific transcription factor GCM	PANTHER	PTHR12414	GLIAL CELLS MISSING RELATED/GLIDE	coord: 78..228
IPR036115	GCM domain superfamily	SUPERFAMILY	SSF90073	GCM domain	coord: 79..231

Main menu

GCM domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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