SMED30030833

Overview
NameSMED30030833
Smed IDSMED30030833
Length (bp)1850
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30030833 (SMED30030833) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30030833 (SMED30030833) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30030833 (SMED30030833) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30030833 (SMED30030833) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030833

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30030833h1SMcG0004818 SmedASXL_009095SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30030833h1SMcG0004818 dd_Smed_v4_10864_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030833h1SMcG0004818 dd_Smed_v4_10864_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30030833h1SMcG0004818 dd_Smed_v4_10864_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30030833h1SMcG0004818 dd_Smed_v6_10864_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30030833h1SMcG0004818 OX_Smed_1.0.10860ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30030833 vs. Ensembl Fly
Match: tinc (gene:FBgn0261649 transcript:FBtr0337039)

HSP 1 Score: 55.4546 bits (132), Expect = 1.370e-7
Identity = 55/205 (26.83%), Postives = 91/205 (44.39%), Query Frame = 1 
Query:  628 EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYS----------VFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTM------NFCLIYEHGRL--RFLD-SIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLY---ILDICISLTHCLLWMIIWILFTFKSEWEFTV 1176
            E  N L A    +VR P +F +TSK+F  ++S          +  ++G +N +   I  + +  +   L L+ A+  +A++ +ST           +Y HGRL  R  D SI + KT        ++ I F + +         L F+L +A +   L      T  N L+    L   + +TH LLW++IW+  T K  W F +
Sbjct:  669 EFVNLLCALLVWSVRYPAVFWNTSKAFACVFSLQMVVAALDIILGYVGISNLYKLQIYAEAMPVHQPGLILN-AVVTLALYLLSTALVLASSMVMYLYGHGRLATRMRDRSIITLKT-------HQTWIYFAHCA--------SLCFVLALAVVKAPLLNDLSATYKNNLHCPTFLAALVGVTHLLLWIVIWLCLTIKRRWHFKL 857
[Close]
BLAST of SMED30030833 vs. Ensembl Fly
Match: tinc (gene:FBgn0261649 transcript:FBtr0304905)

HSP 1 Score: 55.4546 bits (132), Expect = 1.370e-7
Identity = 55/205 (26.83%), Postives = 91/205 (44.39%), Query Frame = 1 
Query:  628 EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYS----------VFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTM------NFCLIYEHGRL--RFLD-SIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLY---ILDICISLTHCLLWMIIWILFTFKSEWEFTV 1176
            E  N L A    +VR P +F +TSK+F  ++S          +  ++G +N +   I  + +  +   L L+ A+  +A++ +ST           +Y HGRL  R  D SI + KT        ++ I F + +         L F+L +A +   L      T  N L+    L   + +TH LLW++IW+  T K  W F +
Sbjct:  669 EFVNLLCALLVWSVRYPAVFWNTSKAFACVFSLQMVVAALDIILGYVGISNLYKLQIYAEAMPVHQPGLILN-AVVTLALYLLSTALVLASSMVMYLYGHGRLATRMRDRSIITLKT-------HQTWIYFAHCA--------SLCFVLALAVVKAPLLNDLSATYKNNLHCPTFLAALVGVTHLLLWIVIWLCLTIKRRWHFKL 857
[Close]
BLAST of SMED30030833 vs. Ensembl Fly
Match: tinc (gene:FBgn0261649 transcript:FBtr0337038)

HSP 1 Score: 55.4546 bits (132), Expect = 1.370e-7
Identity = 55/205 (26.83%), Postives = 91/205 (44.39%), Query Frame = 1 
Query:  628 EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYS----------VFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTM------NFCLIYEHGRL--RFLD-SIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLY---ILDICISLTHCLLWMIIWILFTFKSEWEFTV 1176
            E  N L A    +VR P +F +TSK+F  ++S          +  ++G +N +   I  + +  +   L L+ A+  +A++ +ST           +Y HGRL  R  D SI + KT        ++ I F + +         L F+L +A +   L      T  N L+    L   + +TH LLW++IW+  T K  W F +
Sbjct:  669 EFVNLLCALLVWSVRYPAVFWNTSKAFACVFSLQMVVAALDIILGYVGISNLYKLQIYAEAMPVHQPGLILN-AVVTLALYLLSTALVLASSMVMYLYGHGRLATRMRDRSIITLKT-------HQTWIYFAHCA--------SLCFVLALAVVKAPLLNDLSATYKNNLHCPTFLAALVGVTHLLLWIVIWLCLTIKRRWHFKL 857
[Close]
BLAST of SMED30030833 vs. Ensembl Fly
Match: tinc (gene:FBgn0261649 transcript:FBtr0089771)

HSP 1 Score: 55.4546 bits (132), Expect = 1.370e-7
Identity = 55/205 (26.83%), Postives = 91/205 (44.39%), Query Frame = 1 
Query:  628 EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYS----------VFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTM------NFCLIYEHGRL--RFLD-SIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLY---ILDICISLTHCLLWMIIWILFTFKSEWEFTV 1176
            E  N L A    +VR P +F +TSK+F  ++S          +  ++G +N +   I  + +  +   L L+ A+  +A++ +ST           +Y HGRL  R  D SI + KT        ++ I F + +         L F+L +A +   L      T  N L+    L   + +TH LLW++IW+  T K  W F +
Sbjct:  669 EFVNLLCALLVWSVRYPAVFWNTSKAFACVFSLQMVVAALDIILGYVGISNLYKLQIYAEAMPVHQPGLILN-AVVTLALYLLSTALVLASSMVMYLYGHGRLATRMRDRSIITLKT-------HQTWIYFAHCA--------SLCFVLALAVVKAPLLNDLSATYKNNLHCPTFLAALVGVTHLLLWIVIWLCLTIKRRWHFKL 857
[Close]
BLAST of SMED30030833 vs. Ensembl Fly
Match: tinc (gene:FBgn0261649 transcript:FBtr0089772)

HSP 1 Score: 55.0694 bits (131), Expect = 1.810e-7
Identity = 55/205 (26.83%), Postives = 91/205 (44.39%), Query Frame = 1 
Query:  628 EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYS----------VFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTM------NFCLIYEHGRL--RFLD-SIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLY---ILDICISLTHCLLWMIIWILFTFKSEWEFTV 1176
            E  N L A    +VR P +F +TSK+F  ++S          +  ++G +N +   I  + +  +   L L+ A+  +A++ +ST           +Y HGRL  R  D SI + KT        ++ I F + +         L F+L +A +   L      T  N L+    L   + +TH LLW++IW+  T K  W F +
Sbjct:  669 EFVNLLCALLVWSVRYPAVFWNTSKAFACVFSLQMVVAALDIILGYVGISNLYKLQIYAEAMPVHQPGLILN-AVVTLALYLLSTALVLASSMVMYLYGHGRLATRMRDRSIITLKT-------HQTWIYFAHCAS--------LCFVLALAVVKAPLLNDLSATYKNNLHCPTFLAALVGVTHLLLWIVIWLCLTIKRRWHFKL 857
[Close]
BLAST of SMED30030833 vs. UniProt/SwissProt
Match: sp|Q86B91|TINC_DROME (Protein tincar OS=Drosophila melanogaster OX=7227 GN=tinc PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.373e-6
Identity = 55/205 (26.83%), Postives = 91/205 (44.39%), Query Frame = 1 
Query:  628 EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYS----------VFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTM------NFCLIYEHGRL--RFLD-SIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLY---ILDICISLTHCLLWMIIWILFTFKSEWEFTV 1176
            E  N L A    +VR P +F +TSK+F  ++S          +  ++G +N +   I  + +  +   L L+ A+  +A++ +ST           +Y HGRL  R  D SI + KT        ++ I F + +         L F+L +A +   L      T  N L+    L   + +TH LLW++IW+  T K  W F +
Sbjct:  669 EFVNLLCALLVWSVRYPAVFWNTSKAFACVFSLQMVVAALDIILGYVGISNLYKLQIYAEAMPVHQPGLILN-AVVTLALYLLSTALVLASSMVMYLYGHGRLATRMRDRSIITLKT-------HQTWIYFAHCA--------SLCFVLALAVVKAPLLNDLSATYKNNLHCPTFLAALVGVTHLLLWIVIWLCLTIKRRWHFKL 857
[Close]
BLAST of SMED30030833 vs. TrEMBL
Match: A0A267E669 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030458g3 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 5.970e-6
Identity = 88/379 (23.22%), Postives = 163/379 (43.01%), Query Frame = 1 
Query:  157 WCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGR----IPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHG--------------ISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFI---------GTTNCFFHAITNQVIVSYISTLNLH-IAISNIAIFCISTMNFCLIYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAE-----KYWPNILSFLLWVAFI----ALFLDQYFMNTIFNLLYILDICISLTHCL-LWMIIWILFTFKSEWEFTVH 1179
            W   + +    + +Y A  RY  F+  +   +    W  + + FYL  ++++  L   FLY+ + +T + A D V++GR    +     E  +     A  G  + R+R HF+P+  L++++ AF LL+P+P+L   E  H               I H     + S+      PE+ N +LA   L VR P+ F  ++++F  ++SV + +           T+  F A  +  +V+ ++ L    IA+ +I    +   N    +E G  + L ++   +   Q +ASS      ++    E      Y P++   LL V  +     +  D   +  +     +L   +S    L LW+ +W L  FK+ W+F + 
Sbjct:   48 WYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMILTVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDFIYHPKASATNSV-----CPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKASMSNGVVTQVNLLPPAIIALHSIGDLLLLVANIAF-FEFGYAQMLSNLRKYRNYLQLEASSSVSDRSISSCNDETPCWLNYAPHLAGILLMVVAVLCKAPVLWDWVGLYRVHQQPAMLAAVVSGGIYLSLWVSVWFLLGFKANWKFNIR 416
[Close]
BLAST of SMED30030833 vs. TrEMBL
Match: A0A267GLJ3 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030458g2 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 6.499e-6
Identity = 88/379 (23.22%), Postives = 162/379 (42.74%), Query Frame = 1 
Query:  157 WCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGR----IPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHG--------------ISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFI---------GTTNCFFHAITNQVIVSYISTLNLH-IAISNIAIFCISTMNFCLIYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAE-----KYWPNILSFLLWVAFI----ALFLDQYFMNTIFNLLYILDICISLTHCL-LWMIIWILFTFKSEWEFTVH 1179
            W   + +    + +Y A  RY  F+  +        W  + + FYL  ++++  L   FLY+ + +T + A D V++GR    +     E  +     A  G  + R+R HF+P+  L++++ AF LL+P+P+L   E  H               I H     + S+      PE+ N +LA   L VR P+ F  ++++F  ++SV + +           T+  F A  +  +V+ ++ L    IA+ +I    +   N    +E G  + L ++   +   Q +ASS      ++    E      Y P++   LL V  +     +  D   +  +     +L   +S    L LW+ +W L  FK+ W+F + 
Sbjct:   48 WYAILVLALNAFLLYSAFQRYASFRAKA----LREQWDMSPMNFYLAMMILTVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDFIYHPKASATNSV-----CPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKASMSNDVVTQVNLLPPAIIALHSIGDLLLLVANIAF-FEFGYAQMLSNLRKYRNYLQLEASSSVSDRSISSCNDETPCWLNYAPHLAGILLMVVAVLCKAPVLWDWVGLYRVHQQPAMLAAVVSGGIYLSLWVSVWFLLGFKANWKFNIR 416
[Close]
BLAST of SMED30030833 vs. TrEMBL
Match: A0A267H7U1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030458g1 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 6.591e-6
Identity = 89/379 (23.48%), Postives = 163/379 (43.01%), Query Frame = 1 
Query:  157 WCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGR----IPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHG--------------ISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFI---------GTTNCFFHAITNQVIVSYISTLNLH-IAISNIAIFCISTMNFCLIYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAE-----KYWPNILSFLLWVAFI----ALFLDQYFMNTIFNLLYILDICISLTHCL-LWMIIWILFTFKSEWEFTVH 1179
            W   + +    + +Y A  RY  F+  +   +    W  + + FYL  ++I+  L   FLY+ + +T + A D V++GR    +     E  +     A  G  + R+R HF+P+  L++++ AF LL+P+P+L   E  H               I H     + S+      PE+ N +LA   L VR P+ F  ++++F  ++SV + +           T+  F A  +  +V+ ++ L    IA+ +I    +   N    +E G  + L ++   +   Q +ASS      ++    E      Y P++   LL V  +     +  D   +  +     +L   +S    L LW+ +W L  FK+ W+F + 
Sbjct:   15 WYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMIITVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDFIYHPKASATNSV-----CPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKASMSNGVVTQVNLLPPAIIALHSIGDLLLLVANIAF-FEFGYAQMLSNLRKYRNYLQLEASSSVSDRSISSCNDETPCWLNYAPHLAGILLMVVAVLCKAPVLWDWVGLYRVHQQPAMLAAVVSGGIYLSLWVSVWFLLGFKANWKFNIR 383
[Close]
BLAST of SMED30030833 vs. Planmine SMEST
Match: SMESG000032988.1 (SMESG000032988.1)

HSP 1 Score: 1038.48 bits (2684), Expect = 0.000e+0
Identity = 565/567 (99.65%), Postives = 566/567 (99.82%), Query Frame = 1 
Query:  118 MARWHNCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGRIPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHGISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTMNFCLIYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLYILDICISLTHCLLWMIIWILFTFKSEWEFTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQAXXXXXXXXXXXXKTPNHEXXXXXXXXXXXXXEKFPIQNFIPSISKQLEMSGSNYDDLGTLKSTLRPSRMALKEISEENPEYKEMYQIRSEVSPSDKDYFKSPNVSQRSSNIHSSDSGIGINYRENSDYVYTNTIPENEILKYRSFSPHGIFTCKTHEYNIDEELLDTCDLTNTNMCSDV 1818
            MARWHNCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYLGSILISCLLWLLFLYSTVFQTGHLANDYVELGRIPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFLLLVPIPLLIEEENRHGISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTMNFCLIYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLYILDICISLTHCLLWMIIW+LFTFKSEWEFTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQASDQIQSLSSHSSKTPNHEISSASSSSSSRISEKFPIQNFIPSISKQLEMS SNYDDLGTLKSTLRPSRMALKEISEENPEYKEMYQIRSEVSPSDKDYFKSPNVSQRSSNIHSSDSGIGINYRENSDYVYTNTIPENEILKYRSFSPHGIFTCKTHEYNIDEELLDTCDLTNTNMCSDV
Sbjct:    1 MARWHNCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYLGSILISCLLWLLFLYSTVFQTGHLANDYVELGRIPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFLLLVPIPLLIEEENRHGISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTMNFCLIYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIFNLLYILDICISLTHCLLWMIIWVLFTFKSEWEFTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQASDQIQSLSSHSSKTPNHEISSASSSSSSRISEKFPIQNFIPSISKQLEMSDSNYDDLGTLKSTLRPSRMALKEISEENPEYKEMYQIRSEVSPSDKDYFKSPNVSQRSSNIHSSDSGIGINYRENSDYVYTNTIPENEILKYRSFSPHGIFTCKTHEYNIDEELLDTCDLTNTNMCSDV 567
[Close]
BLAST of SMED30030833 vs. Planmine SMEST
Match: SMESG000077784.1 (SMESG000077784.1)

HSP 1 Score: 68.9366 bits (167), Expect = 6.377e-12
Identity = 103/433 (23.79%), Postives = 184/433 (42.49%), Query Frame = 1 
Query:  133 NCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGRIPSVADEIINSIDCLAVCGRILKRIRYHFVPFNSLIYL-IGAFXXXXXXXXXXXXXNRHGIS---------------HFGNDQSESLLLCIYIP------------EITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISN-------IAIFCISTM----NFCLIYEHGRLRFLDSIFS-RKTGSQDQASSKSGIVF-VNKSPAEKYWPNILSFLLWVAFIALFLDQYFMNTIF---------NLLYILDICISLTHCLLWMIIWILFTFKSEWEFTVHQQ--PIKVLGISRDHSFSAPKCFVKQCDTIYTCF 1275
            NC  L   W + + I+  +  I +A + Y  +K  S +P+FG +W    + F L SI++S  L +  +YS +F+ GH+AND + LG       + +  I         L R+  H +P+N  I+L I   L L    +  E+     IS               H       + ++C  +P            E  N +++   + +RLP+ F  T+K+F I++S+F FI  +       +  +I+  I   +L   I +       + IF I ++       +I+E+G  +F+ ++   R+  S D + S S      N+       P I   L+ V    L +   ++   +         + L ++ ICISL + L  + +W     K++W+F ++ Q  P+  +G+ +  S   P         IY CF
Sbjct:   10 NCDRLWKLWYSILIIVMQVLLISLAISNYLTYKSQSFDPRFGNHWSALPLNFQLASIVLSLSLLVWLIYSCMFKVGHIANDGIVLGLDLDSIKKALEEISSYKGIKLFLTRLITHVLPYNCFIHLSIALLLCLPVALMQAEQIKNEAISPDYIWTTNIDFLISPHSFRIFKANTVICYKLPCEYPTKPSAIAPEFINLIISLLIIVIRLPSPFWYTNKAFSILFSIFAFITVSCILMEYESLILIIKIIHNYDLDTKIESHLSVWLILIIFLIKSLLMIFMLIIIFEYGYTQFISNMRKYRQYFSSDISDSVSQQSLNDNQYKWYNVSPRIFGILILVIVKILKIPILYVQISYLKINGKISIHPLILISICISLFYFLSCLFLWFGLCVKNKWQFHINIQTNPLPQIGVGKLKSSPEP---------IYGCF 433
[Close]
BLAST of SMED30030833 vs. Planmine SMEST
Match: SMESG000005995.1 (SMESG000005995.1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.202e-10
Identity = 112/453 (24.72%), Postives = 181/453 (39.96%), Query Frame = 1 
Query:  133 NCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGRIPSVA---------DEIINSIDCLA--VCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHGISHFGN----------------------DQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVF-IFIGTTNCFFHAITNQVIVSYISTLNLHIAISN-------------IAIFCISTMNFCLIYEHGRLRFLDSIFS-RKTGSQDQASSKSGIVFVN--------KSPAEKYWPNILSFLLWVA-----------FIALFLD---QYFMNTIFNLLYILDICISLTHCLLWMIIWILFTFKSEWEFTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQA 1281
            NC +L   W     + F +Y  Y     Y  FK  S +P++G  W EN + F L   + S  L  +FLY+ + +TG+L ND V+LG   ++          + I N  D  A   C  I K   Y+  P   L++ I AFLLLVP   L  E+ ++    + N                       +SESL   I      NF +A+  L +   ++F  T KSF I+ ++F + +       +++ + VI  Y+S  N  +  S+             IA F  S +  C+I+E G  R+L +  S  +   + +  S S  +F N        +   + +W N L F L V            F+  + D    +F+   F + +I+         + W  +W+  + K  W F ++      L       F+         + IYTCF  
Sbjct:   11 NCRSLGKIWYGFTILAFQIYVHYDEINNYLDFKKQSNDPRYGSEWNENPLTFCLMMTMFSFALLPIFLYTCLIKTGNLINDNVDLGETNTLQILQPDSLIFERIQNCHDDSAKPKCWNIFK---YYVFPSCQLLHYISAFLLLVPHLYLNAEKFKNSALSYENLWISDLHLIFNVRDPGTLTIVKQESESLSFPI---STINFFIASMFLALFTSSVFWKTCKSFSILIAIFDLVLSFGYLILNSVLSIVIRYYLSLENPSLKSSSKPLYSQWQCACLLIAGFISSLICRCIIFEFGLQRYLTNQRSFDQLLEEVKVPSISTGLFHNSRELKNRLRGQFQNFWLNALGFFLLVIEGGCLIPLACYFVTSYWDCTKTFFLFGFFAVTFII---------ITWFFVWMTLSVKHNWIFKIN------LSTKNQKMFNV--------EPIYTCFNG 434
[Close]
BLAST of SMED30030833 vs. Planmine SMEST
Match: SMESG000005995.1 (SMESG000005995.1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.519e-10
Identity = 112/453 (24.72%), Postives = 181/453 (39.96%), Query Frame = 1 
Query:  133 NCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGRIPSVA---------DEIINSIDCLA--VCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHGISHFGN----------------------DQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFIGTTN-CFFHAITNQVIVSYISTLNLHIAISN-------------IAIFCISTMNFCLIYEHGRLRFLDSIFS-RKTGSQDQASSKSGIVFVN--------KSPAEKYWPNILSFLLWVA-----------FIALFLD---QYFMNTIFNLLYILDICISLTHCLLWMIIWILFTFKSEWEFTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQA 1281
            NC +L   W     + F +Y  Y     Y  FK  S +P++G  W EN + F L   + S  L  +FLY+ + +TG+L ND V+LG   ++          + I N  D  A   C  I K   Y+  P   L++ I AFLLLVP   L  E+ ++    + N                       +SESL   I      NF +A+  L +   ++F  T KSF I+ ++F  + +      +++ + VI  Y+S  N  +  S+             IA F  S +  C+I+E G  R+L +  S  +   + +  S S  +F N        +   + +W N L F L V            F+  + D    +F+   F + +I+         + W  +W+  + K  W F ++      L       F+         + IYTCF  
Sbjct:   11 NCRSLGKIWYGFTILAFQIYVHYDEINNYLDFKKQSNDPRYGSEWNENPLTFCLMMTMFSFALLPIFLYTCLIKTGNLINDNVDLGETNTLQILQPDSLIFERIQNCHDDSAKPKCWNIFK---YYVFPSCQLLHYISAFLLLVPHLYLNAEKFKNSALSYENLWISDLHLIFNVRDPGTLTIVKQESESLSFPI---STINFFIASMFLALFTSSVFWKTCKSFSILIAIFDLVLSFGYLILNSVLSIVIRYYLSLENPSLKSSSKPLYSQWQCACLLIAGFISSLICRCIIFEFGLQRYLTNQRSFDQLLEEVKVPSISTGLFHNSRELKNRLRGQFQNFWLNALGFFLLVIEGGCLIPLACYFVTSYWDCTKTFFLFGFFAVTFII---------ITWFFVWMTLSVKHNWIFKIN------LSTKNQKMFNV--------EPIYTCFNG 434
[Close]
BLAST of SMED30030833 vs. Planmine SMEST
Match: SMESG000005995.1 (SMESG000005995.1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.874e-10
Identity = 112/453 (24.72%), Postives = 181/453 (39.96%), Query Frame = 1 
Query:  133 NCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNWKENYIVFYXXXXXXXXXXXXXXXYSTVFQTGHLANDYVELGRIPSVA---------DEIINSIDCLA--VCGRILKRIRYHFVPFNSLIYLIGAFXXXXXXXXXXXXXNRHGISHFGN----------------------DQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGIIYSVFIFIGTTN-CFFHAITNQVIVSYISTLNLHIAISN-------------IAIFCISTMNFCLIYEHGRLRFLDSIFS-RKTGSQDQASSKSGIVFVN--------KSPAEKYWPNILSFLLWVA-----------FIALFLD---QYFMNTIFNLLYILDICISLTHCLLWMIIWILFTFKSEWEFTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQA 1281
            NC +L   W     + F +Y  Y     Y  FK  S +P++G  W EN + F L   + S  L  +FLY+ + +TG+L ND V+LG   ++          + I N  D  A   C  I K   Y+  P   L++ I AFLLLVP   L  E+ ++    + N                       +SESL   I      NF +A+  L +   ++F  T KSF I+ ++F  + +      +++ + VI  Y+S  N  +  S+             IA F  S +  C+I+E G  R+L +  S  +   + +  S S  +F N        +   + +W N L F L V            F+  + D    +F+   F + +I+         + W  +W+  + K  W F ++      L       F+         + IYTCF  
Sbjct:   11 NCRSLGKIWYGFTILAFQIYVHYDEINNYLDFKKQSNDPRYGSEWNENPLTFCLMMTMFSFALLPIFLYTCLIKTGNLINDNVDLGETNTLQILQPDSLIFERIQNCHDDSAKPKCWNIFK---YYVFPSCQLLHYISAFLLLVPHLYLNAEKFKNSALSYENLWISDLHLIFNVRDPGTLTIVKQESESLSFPI---STINFFIASMFLALFTSSVFWKTCKSFSILIAIFDLVLSFGYLILNSVLSIVIRYYLSLENPSLKSSSKPLYSQWQCACLLIAGFISSLICRCIIFEFGLQRYLTNQRSFDQLLEEVKVPSISTGLFHNSRELKNRLRGQFQNFWLNALGFFLLVIEGGCLIPLACYFVTSYWDCTKTFFLFGFFAVTFII---------ITWFFVWMTLSVKHNWIFKIN------LSTKNQKMFNV--------EPIYTCFNG 434
The following BLAST results are available for this feature:
BLAST of SMED30030833 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tinc1.370e-726.83gene:FBgn0261649 transcript:FBtr0337039[more]
tinc1.370e-726.83gene:FBgn0261649 transcript:FBtr0304905[more]
tinc1.370e-726.83gene:FBgn0261649 transcript:FBtr0337038[more]
tinc1.370e-726.83gene:FBgn0261649 transcript:FBtr0089771[more]
tinc1.810e-726.83gene:FBgn0261649 transcript:FBtr0089772[more]
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BLAST of SMED30030833 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q86B91|TINC_DROME1.373e-626.83Protein tincar OS=Drosophila melanogaster OX=7227 ... [more]
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BLAST of SMED30030833 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A267E6695.970e-623.22Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267GLJ36.499e-623.22Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267H7U16.591e-623.48Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30030833 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030833 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032988.10.000e+099.65SMESG000032988.1[more]
SMESG000077784.16.377e-1223.79SMESG000077784.1[more]
SMESG000005995.11.202e-1024.72SMESG000005995.1[more]
SMESG000005995.11.519e-1024.72SMESG000005995.1[more]
SMESG000005995.11.874e-1024.72SMESG000005995.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030833 ID=SMED30030833|Name=SMED30030833|organism=Schmidtea mediterranea sexual|type=transcript|length=1850bp
TTCAGTTGTGACAACAATTACAATCATGGAGAGGAAATTATTTAATTTCT
TTCTTTTTCTCCCTAATTGAACAATGAGTCACATATTGATTATTATTCCA
CTGTCAAATGCATAGAAATGGCTCGCTGGCACAATTGTCCGAATCTCGCG
GCATTTTGGTGCAATGGGATCTTTATCATCTTCTATCTCTACACGATTTA
CGTTGCTTGCACTAGATACAATCAGTTCAAAGTCAATTCCGGCAATCCGA
AATTTGGATTCAATTGGAAAGAGAATTACATAGTGTTTTATTTGGGTTCA
ATTCTAATCTCATGCCTGCTGTGGCTGCTATTTCTGTACTCAACTGTTTT
CCAAACAGGCCACCTCGCCAATGACTATGTGGAATTGGGGAGAATCCCAT
CAGTAGCCGATGAGATCATTAACTCTATCGACTGCTTGGCGGTTTGCGGA
AGAATCCTGAAGCGTATCCGATATCATTTTGTTCCATTTAATTCTTTAAT
TTATTTAATTGGCGCTTTTCTACTATTAGTTCCGATTCCACTTTTAATCG
AGGAAGAAAATCGACATGGAATTTCACATTTCGGCAACGATCAGTCAGAA
TCTCTTCTCTTATGTATTTACATACCAGAAATTACAAATTTCCTTCTTGC
AGCATTCTCCTTGACTGTCCGACTCCCAACATTGTTTAGCAGCACGTCAA
AATCGTTTGGAATAATATATTCTGTTTTTATATTTATCGGAACAACAAAT
TGTTTTTTCCACGCAATAACAAATCAAGTCATTGTGTCATACATATCAAC
ATTAAATCTACATATCGCAATATCTAATATCGCTATTTTCTGCATATCCA
CAATGAATTTCTGTTTAATTTATGAGCACGGTCGTTTGAGATTTCTTGAC
TCTATTTTTTCCCGAAAAACCGGATCTCAAGATCAGGCCAGCTCGAAGTC
AGGCATTGTTTTTGTCAACAAATCGCCAGCAGAGAAATATTGGCCGAATA
TTTTGTCATTTCTTTTATGGGTGGCATTTATTGCACTATTTTTAGATCAA
TATTTCATGAACACTATTTTCAATTTGCTTTATATATTAGACATTTGCAT
TTCTTTGACTCATTGTTTACTCTGGATGATTATATGGATATTGTTTACTT
TCAAATCTGAATGGGAATTCACGGTTCACCAGCAGCCGATAAAGGTCTTA
GGAATAAGTCGGGATCATTCATTTTCCGCTCCAAAATGTTTTGTAAAACA
ATGCGACACGATTTATACTTGTTTCCAGGCGTCAGATCAAATACAATCTT
TGTCGTCCCATTCGTCGAAAACACCGAACCATGAAATTTCTTCTGCCAGC
AGTAGCAGCAGCAGTAGAATATCAGAAAAATTTCCAATTCAAAATTTTAT
ACCGAGTATTTCGAAACAACTGGAAATGTCAGGCAGTAACTATGACGATT
TAGGAACTCTCAAATCCACACTAAGACCATCAAGAATGGCTTTGAAAGAA
ATTTCTGAAGAAAACCCTGAGTATAAAGAAATGTATCAAATTCGTTCGGA
AGTTTCTCCAAGTGATAAAGACTATTTCAAAAGTCCCAATGTTTCTCAGA
GAAGCTCGAATATCCACAGCAGTGACAGTGGAATCGGAATAAATTATCGC
GAAAATTCCGATTATGTTTATACAAATACAATACCGGAAAATGAAATTTT
GAAATACCGTTCATTTTCTCCTCATGGAATTTTTACCTGTAAGACTCACG
AATATAATATCGACGAAGAACTGCTAGATACCTGTGACTTGACCAATACT
AATATGTGCAGTGATGTGTAGCACTCCTTGTCAATCGAGCGAATCATAAC
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protein sequence of SMED30030833-orf-1

>SMED30030833-orf-1 ID=SMED30030833-orf-1|Name=SMED30030833-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=568bp
MARWHNCPNLAAFWCNGIFIIFYLYTIYVACTRYNQFKVNSGNPKFGFNW
KENYIVFYLGSILISCLLWLLFLYSTVFQTGHLANDYVELGRIPSVADEI
INSIDCLAVCGRILKRIRYHFVPFNSLIYLIGAFLLLVPIPLLIEEENRH
GISHFGNDQSESLLLCIYIPEITNFLLAAFSLTVRLPTLFSSTSKSFGII
YSVFIFIGTTNCFFHAITNQVIVSYISTLNLHIAISNIAIFCISTMNFCL
IYEHGRLRFLDSIFSRKTGSQDQASSKSGIVFVNKSPAEKYWPNILSFLL
WVAFIALFLDQYFMNTIFNLLYILDICISLTHCLLWMIIWILFTFKSEWE
FTVHQQPIKVLGISRDHSFSAPKCFVKQCDTIYTCFQASDQIQSLSSHSS
KTPNHEISSASSSSSSRISEKFPIQNFIPSISKQLEMSGSNYDDLGTLKS
TLRPSRMALKEISEENPEYKEMYQIRSEVSPSDKDYFKSPNVSQRSSNIH
SSDSGIGINYRENSDYVYTNTIPENEILKYRSFSPHGIFTCKTHEYNIDE
ELLDTCDLTNTNMCSDV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 396..416
NoneNo IPR availablePANTHERPTHR21579FAMILY NOT NAMEDcoord: 11..501
NoneNo IPR availableTMHMMTMhelixcoord: 230..252
NoneNo IPR availableTMHMMTMhelixcoord: 53..75
NoneNo IPR availableTMHMMTMhelixcoord: 196..218
NoneNo IPR availableTMHMMTMhelixcoord: 164..186
NoneNo IPR availableTMHMMTMhelixcoord: 291..313
NoneNo IPR availableTMHMMTMhelixcoord: 10..32
NoneNo IPR availableTMHMMTMhelixcoord: 320..342
NoneNo IPR availableTMHMMTMhelixcoord: 121..143