SMED30030788

Overview
NameSMED30030788
Smed IDSMED30030788
Length (bp)793
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30030788 (SMED30030788) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30030788 (SMED30030788) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30030788 (SMED30030788) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30030788 (SMED30030788) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030788

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30030788SMESG000079496.1 Contig2447GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30030788SMESG000079496.1 dd_Smed_v4_6860_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030788SMESG000079496.1 SMESG000051017.1 SMESG000043567.1 SMESG000035748.1 SMESG000032351.1 SMESG000029267.1 SMESG000023200.1 SMESG000021127.1 SMESG000020356.1 Contig36498newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030788SMESG000079496.1 SMESG000051017.1 SMESG000043567.1 SMESG000035748.1 SMESG000032351.1 SMESG000029267.1 SMESG000023200.1 SMESG000021127.1 SMESG000020356.1 Contig36498uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
tail regionSMED30030788SMESG000079496.1 dd_Smed_v6_6860_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30030788 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000621:38312..47831 -3891
v31.000621:63946..65860 -3443
Homology
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BLAST of SMED30030788 vs. Planmine SMEST
Match: SMESG000079496.1 (SMESG000079496.1)

HSP 1 Score: 377.481 bits (968), Expect = 1.627e-134
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 2 
Query:  191 MMKANCCCQKVSFSDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINASHCKN 733
            MMKANCCCQKVSFSDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINASHCKN
Sbjct:    1 MMKANCCCQKVSFSDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINASHCKN 181
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BLAST of SMED30030788 vs. Planmine SMEST
Match: SMESG000079496.1 (SMESG000079496.1)

HSP 1 Score: 374.785 bits (961), Expect = 1.453e-133
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 2 
Query:  194 MKANCCCQKVSFSDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINASHCKN 733
            MKANCCCQKVSFSDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINASHCKN
Sbjct:    1 MKANCCCQKVSFSDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINASHCKN 180
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BLAST of SMED30030788 vs. Planmine SMEST
Match: SMESG000079498.1 (SMESG000079498.1)

HSP 1 Score: 165.236 bits (417), Expect = 4.598e-51
Identity = 83/159 (52.20%), Postives = 107/159 (67.30%), Query Frame = 2 
Query:  230 SDSTNWVYSCKNDEYKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSA 706
             D T   Y C ND+Y +L A+ +GSDL+L     ++      + F+E HY   D  +P+SVIQH+VS KA+FYD   + F LKS  +N++ ITDPNSNFLL+VGGGSTV CFSN++TT TLA VG NL TY NTT PPW  S   L++S  DF+WIR+ 
Sbjct:   10 GDFTECTYCCYNDKYYWLTANTDGSDLRLLNTKTEAGHVIEPFMFKELHYHSED-GIPISVIQHEVSGKALFYDDENKTFELKSSEDNLDNITDPNSNFLLYVGGGSTVTCFSNKKTTGTLAYVGLNLITYENTTSPPWTLSQDTLEDSRNDFSWIRNT 167
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BLAST of SMED30030788 vs. Planmine SMEST
Match: SMESG000025939.1 (SMESG000025939.1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.209e-16
Identity = 62/195 (31.79%), Postives = 99/195 (50.77%), Query Frame = 2 
Query:  149 LFIFY-HSIDPTWSCMMKANCCCQKVSFSD----STNWVYSCKNDEYKYLKADANGSDLKL--WTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPE-NIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAAL-QNSTKDFTWIRSA 706
            L +F+  +I+     ++  NC   K S S     S  +V+ C  +E  YL AD++   L+L  ++M++ +     N  F++Y+   +  NV +SVI+HK S K I+YD  ++ F+LK  PE + +K    +SNF+L  GG S + C SN  T  TLA       T ++    PW YS   L  +  K   W + +
Sbjct:    4 LLLFHKQNINIEEFSLIMGNCIHFKTSGSSHHLSSNEYVFCCYLNENAYLVADSDMVHLQLTNFSMLQST----HNVIFQQYYLDSD--NVHLSVIKHKRSGKFIYYDEDSKDFILKENPEVHFDKF---DSNFILARGGNSNIFCLSNLETNATLAYNNGQFQT-LHIPEKPWLYSSEDLIDDKMKHIAWKKKS 188
[Close]
BLAST of SMED30030788 vs. Planmine SMEST
Match: SMESG000025939.1 (SMESG000025939.1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.676e-15
Identity = 59/177 (33.33%), Postives = 90/177 (50.85%), Query Frame = 2 
Query:  200 ANCCCQKVSFSD----STNWVYSCKNDEYKYLKADANGSDLKL--WTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQHKVSKKAIFYDGTTERFVLKSVPE-NIEKITDPNSNFLLFVGGGSTVACFSNRRTTRTLACVGKNLTTYVNTTYPPWKYSPAAL-QNSTKDFTWIRSA 706
             NC   K S S     S  +V+ C  +E  YL AD++   L+L  ++M++ +     N  F++Y+   +  NV +SVI+HK S K I+YD  ++ F+LK  PE + +K    +SNF+L  GG S + C SN  T  TLA       T ++    PW YS   L  +  K   W + +
Sbjct:    2 GNCIHFKTSGSSHHLSSNEYVFCCYLNENAYLVADSDMVHLQLTNFSMLQST----HNVIFQQYYLDSD--NVHLSVIKHKRSGKFIYYDEDSKDFILKENPEVHFDKF---DSNFILARGGNSNIFCLSNLETNATLAYNNGQFQT-LHIPEKPWLYSSEDLIDDKMKHIAWKKKS 168
The following BLAST results are available for this feature:
BLAST of SMED30030788 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030788 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079496.11.627e-134100.00SMESG000079496.1[more]
SMESG000079496.11.453e-133100.00SMESG000079496.1[more]
SMESG000079498.14.598e-5152.20SMESG000079498.1[more]
SMESG000025939.16.209e-1631.79SMESG000025939.1[more]
SMESG000025939.11.676e-1533.33SMESG000025939.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030788 ID=SMED30030788|Name=SMED30030788|organism=Schmidtea mediterranea sexual|type=transcript|length=793bp
TGTAACAGACAAATTGAATGGATGCAGATGAACTGCATCAATGCAGTTGG
ACTGCATCAACAGAATGGCATTTTGAATCAAAGTTCAAGTAGTAAATATT
TTCATTTTAAAATCAAATCAAATGAATTTTCTATTACAGGGCGTTTTTTT
ATTTATATTTTATCATTCAATAGACCCAACCTGGTCATGTATGATGAAGG
CAAATTGTTGTTGTCAAAAAGTTTCCTTCAGTGACTCTACAAATTGGGTA
TATTCTTGTAAGAATGACGAATACAAGTATTTGAAGGCAGATGCAAATGG
GAGTGATTTGAAATTGTGGACAATGGTAGAAGACTCTTGTGAAAATTATA
CAAATTATGAGTTCCGAGAATATCATTACCGTTGTAATGACTGCAATGTA
CCCGTATCGGTCATCCAGCATAAAGTCAGTAAGAAGGCCATTTTTTATGA
TGGCACAACAGAACGTTTTGTGTTGAAAAGTGTCCCTGAAAATATAGAAA
AGATCACAGATCCAAATTCAAATTTCTTGTTGTTTGTTGGTGGGGGATCA
ACAGTTGCTTGCTTCTCCAACAGAAGAACGACGAGAACACTCGCTTGTGT
TGGAAAAAATCTTACGACTTATGTAAATACTACTTACCCACCTTGGAAAT
ATTCTCCAGCTGCATTACAAAATTCAACAAAAGATTTTACGTGGATAAGA
AGCGCAAACATTAATGCTTCTCATTGCAAGAACTAATTTGTAATTGAATA
AAAGAAAGTAGATAAAATAAAAGTTTTCATTTTTAAAAAAAAA
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protein sequence of SMED30030788-orf-1

>SMED30030788-orf-1 ID=SMED30030788-orf-1|Name=SMED30030788-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=205bp
MNFLLQGVFLFIFYHSIDPTWSCMMKANCCCQKVSFSDSTNWVYSCKNDE
YKYLKADANGSDLKLWTMVEDSCENYTNYEFREYHYRCNDCNVPVSVIQH
KVSKKAIFYDGTTERFVLKSVPENIEKITDPNSNFLLFVGGGSTVACFSN
RRTTRTLACVGKNLTTYVNTTYPPWKYSPAALQNSTKDFTWIRSANINAS
HCKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
PLANA:0000421tail
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.65