SMED30030769
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030769 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30030769 aligns in the following genomic locations:
Homology
BLAST of SMED30030769 vs. Planmine SMEST
Match: SMESG000027067.1 (SMESG000027067.1) HSP 1 Score: 944.88 bits (2441), Expect = 0.000e+0 Identity = 495/498 (99.40%), Postives = 498/498 (100.00%), Query Frame = 3 Query: 78 MAIHIEDHLIPVEXXXXXXXXXXXXXXXSAFYEFFFSQPKVELTMNIFQTVIVAISISAILGNFVLLRQNRDQFYYKGCKNKVVLCSNCVIGLSGVVYSMYELHKENNNLINSHFVDQXXXXXXXXXXXXXXXSSDWIPDIQRPLLDNNFSGICTYRINYWSCLFGTICLIGCFVIFGRIIAIKERQTLNMTVNRNRDFFSCPSYKDPKKLDVKKIFENFLLNYFIISFVLVYIYLRFISRQIRDLHEEWAHLEADHPIRFDQPNEDPNDVRSPLITNNRNPFKRCCNKQIILSAVISISFLISLNTLNIFLIYSDDKVKIHIAYQAVKIIIYLLFIGTFILLWCQLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVIVHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSVTRVASVHWKPLALFLCVMNLSFALENLIMKPMTIQMYNKAWVVADMLINPMKFINRLHAFAFFANILAK 1571 MAIHIEDHLIPVELIKSLKYFLLLLIFSSAFYEFFFSQPKVELTMNIFQTVIVAISISAILGNFVLLRQNRDQFYYKGCKNKVVLCSNCVIGLSGVVYSMYELHKENNNLINSHFVDQIFIIAFAILLALYLYSSDWIPDIQRPLLDNNFSGICTYRINYWSCLFGTICLIGCFVIFGRIIAIKERQTLNMTVNRNRDFFSCPSYKDPKKLDVKKIFENFLLNYFIISFVLVYIYLRFISRQIRDLHEEWAHLEADHPIRFDQPNEDPNDVRSPLITNNRNPFKRCCNKQIILSAVISISFLISLNTLNIFLIYSDDKVKIHIAYQAVKIIIYLLFIGTFILLWCQLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVIVHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSVTRVASVHWKPLALFLCVMNLSFALENLIMKPMTIQMYNKAWVVADMLINP++FINRLHAFAFFANILA+ Sbjct: 1 MAIHIEDHLIPVELIKSLKYFLLLLIFSSAFYEFFFSQPKVELTMNIFQTVIVAISISAILGNFVLLRQNRDQFYYKGCKNKVVLCSNCVIGLSGVVYSMYELHKENNNLINSHFVDQIFIIAFAILLALYLYSSDWIPDIQRPLLDNNFSGICTYRINYWSCLFGTICLIGCFVIFGRIIAIKERQTLNMTVNRNRDFFSCPSYKDPKKLDVKKIFENFLLNYFIISFVLVYIYLRFISRQIRDLHEEWAHLEADHPIRFDQPNEDPNDVRSPLITNNRNPFKRCCNKQIILSAVISISFLISLNTLNIFLIYSDDKVKIHIAYQAVKIIIYLLFIGTFILLWCQLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVIVHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSVTRVASVHWKPLALFLCVMNLSFALENLIMKPMTIQMYNKAWVVADMLINPLRFINRLHAFAFFANILAE 498
BLAST of SMED30030769 vs. Planmine SMEST
Match: SMESG000026752.1 (SMESG000026752.1) HSP 1 Score: 515.383 bits (1326), Expect = 1.430e-179 Identity = 308/515 (59.81%), Postives = 373/515 (72.43%), Query Frame = 3 Query: 90 IEDHLIPVEXXXXXXXXXXXXXXXSAFYEFFFSQPKVELTMNIFQTVIVAISISAILGNFVLLRQNRDQFYYKGCKNKVVLCSNCVIGLSGVVYSMYELHKENNNLINSHFVDQXXXXXXXXXXXXXXXSSDWIPDIQRPLLDNNFSGICTYRINYWSCLFGTICLIGCFVIFGRIIAIKERQTLNMTVNRNRDFFSCPSYKDPKKLDVKKIFENFLLNYFIISFVLVYIYLRFISRQIRDLHEEWA--------------------HLEADHPIRFDQPNEDPNDVRSPLITNNRNPFKRCCNKQIILSAVISISFLISLNTLNIFLIYSDDKVKIHIAYQAVKIIIYLLFIGTFILLWCQLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVIVHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSVTRVASVHWKPLALFLCVMNLSFALENLIMKPMTIQMYNKAWVVADMLINPMKFINRLHAFAFFANILAKQ 1574 I LIPVELIK LKYFLLLLIFSS+ YE F S+P+V MNIFQT++V +SI+A+LGN L +N+ F KNK++LCS VIGL GVV ++YE++ E I ++F+D++ II FAILLA+YLY SDW+P IQRP +D+N +RINY LFGTICL+GCF+IFGRIIAIKER+ L MT N N SC S+KD K++V +I + FL NYFIIS V +++YLRFISRQIRD+HEE A HLE + PIR DQ NE N+ R+PL+ N +P ++CCNKQIILSA++S FLI LN LN I D IHIAYQA+ I+++LL IG + W Q+L W + + +LQ+DNLFLKIGTFGQ LNGCLVLISAFYV + +C + IN LWATSNF+QIFQSIFQMYLI EIS RSV A H KPL LFLCV NLSFALENL MKPMT+ +Y+KAW+VADMLINPMKFINRLHAFAFFANILAKQ Sbjct: 3 INRDLIPVELIKCLKYFLLLLIFSSSLYELFISKPQVSKNMNIFQTIVVGLSIAAVLGNLYLFCKNQVLFEKSEFKNKIILCSTYVIGLVGVVNAIYEINYETYRDI-TYFIDKLVIIIFAILLAIYLYISDWMPHIQRPQVDDNDYKKRVFRINYLISLFGTICLVGCFIIFGRIIAIKERKILTMTENINSSSLSCLSFKDHIKIEVTEILDYFLENYFIISSVFIFVYLRFISRQIRDIHEELAYQQNVDPDVGRVLPNDNERPHLETNDPIRSDQTNESLNE-RTPLV-NITDPPRKCCNKQIILSAIVSGFFLIFLNNLNFLKILKYDF--IHIAYQAINIVVHLLLIGAISMCWHQILYWNNKKSSILQLDNLFLKIGTFGQVLNGCLVLISAFYVESYGNCGGSAVSINALWATSNFLQIFQSIFQMYLIHEISKRSVIENALEHLKPLTLFLCVTNLSFALENLTMKPMTMTVYDKAWIVADMLINPMKFINRLHAFAFFANILAKQ 512
BLAST of SMED30030769 vs. Planmine SMEST
Match: SMESG000071516.1 (SMESG000071516.1) HSP 1 Score: 64.6994 bits (156), Expect = 7.158e-11 Identity = 51/195 (26.15%), Postives = 94/195 (48.21%), Query Frame = 3 Query: 1020 SDDKVKIHIAYQAVKIIIYLLFIGTFILLWCQLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVIVHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSVTRVASVHWKP---------LALFLCVMNLSFALENLIMKPMT---IQMY-NKAWVVADMLINPMKFINRLHAFAFFANIL 1565 ++D I + Y +I +Y++ I +L ++ +W+ E D + IGTFGQ + + +F + + DC Y++++ N +QI Q + Q ++I EI R V ++ S +++ LC+ NL F L N++ IQM + W++ ++ + +NRLH FAFF ++L Sbjct: 287 TNDSQNILVFYNECQISVYVILIIISMLYLHKIFQWKFEKNIRSNNDCWIVNIGTFGQVMEAITISYVSF-IKLSDDCHLKFLYLSIVL---NILQIIQIVGQRFVILEILQRKVKKLRSRNYETNFFRRITINHCAVLCIANLGFVLINILHYSQAMDKIQMSKGRTWILIRCILVLISTLNRLHGFAFFLDVL 477
BLAST of SMED30030769 vs. Planmine SMEST
Match: SMESG000037573.1 (SMESG000037573.1) HSP 1 Score: 51.9878 bits (123), Expect = 8.069e-7 Identity = 94/362 (25.97%), Postives = 153/362 (42.27%), Query Frame = 3 Query: 579 ICLIGCFVIFGRII--AIKE---RQTLNMTVNRNRDFFSCPSYKDPK---KLDVKKIFENFLLNYFIISFVLVYIYLRFISRQIRDLHEEWAHLEADHPIR---FDQPNEDPNDVRSPLITNNRNPFKRCCNKQIILSAVISISFLISLNTLNIFLIYSDDKVKIHIAYQAVKII--IYLLFIGTFILLWC-QLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVI-VHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSVTRVAS---VHWK-----PLAL-----FLCVMNLSFALENLIMK-PMT-IQMYNKAWVVADMLINPMKFINRLHAFAFFANILAKQ 1574 + +IGC +IF R I I E Q+LN T + S +D KL + I E F +NYFII+F+ + L +I D+ +++ H R FD + VR L + + +SFLI ++TL + L S I + +I +YL+ IG I + +L W E+ L+ID F + + Q ++ +L+S V+ ++ D + Y +L +NF+ Q + Q I S R V R + + W+ PL L + + N + + + P T I + W+ L P+ +NR HA +FF N+ KQ Sbjct: 180 LSMIGCMLIFTRKILHKIGEDVISQSLNSTPVISNSCSSSGKLRDIHNICKLPLN-ILEIFDINYFIIAFIFLINPLLYIDTGEDDVTNR--SIQSQHESRSSVFDWISL----VRFRLFS-------------------VFLSFLI-ISTLLVTLYISVSSFDTTIKTILLNLISFVYLIVIGYGIFYYLFDILYWDKEVNRDLKIDQYFNHLTSSAQIIHSSTMLMSLVIVVFMNNKVDLEISYSEIL---NNFLLSVQVLIQASCIHSASERVVRRFVTGEIISWRSVLIPPLVLASFNFIMMITNFLYIFSPFLSRLPQTDINLGRIIWIFIKDLTLPIALLNRFHAMSFFINLTVKQ 511
BLAST of SMED30030769 vs. Planmine SMEST
Match: SMESG000071518.1 (SMESG000071518.1) HSP 1 Score: 48.9062 bits (115), Expect = 7.308e-6 Identity = 74/305 (24.26%), Postives = 118/305 (38.69%), Query Frame = 3 Query: 717 KKIFENFLLNYFIISFVLVYIYLRFISRQIRDLHEEWAHLEADHPIRFDQPNEDPNDVRSPLITNNRNPFKRCCNKQIILSAVISISFLISL--------NTLNIFLIYSDDKVKIHIAYQAVKIIIYLLFIGTFILLWCQLLKWRIEITPLLQIDNLFLKIGTFGQALNGCLVLISAFYVIVHKDCDRTVKYINVLWATSNFMQIFQSIFQMYLIREISNRSV--------TRVASVHWKPL---ALFLCVMNLSFALENLIMKPMTIQMYNKA----WVVADMLINPMKFINRLHAFAFFANI 1562 KK F F + + +S + ++ ++ + + L H D+ D N+ PLI N++ F K S VI I LI+L N +I+L +D +Y + I + I + ++L ++E P +ID L GTF Q + L + K + +N I Q +FQ LI EI R V TR + WK L L +++ F + LI T+ M K W + + P K +NRLH+F+ +I Sbjct: 173 KKEFRLFSIFFLFVSSIFIFFSTHEDVNEVVKMQHTLSCLNNVHE---DEIGLDENEA-DPLIPKNKSEFTMPL-KNNFFSIVIGIGCLIALVIVESSCRNRYSIYLNITDGSNSKCYSYYNNTVAICTISIAFTLWYLHEILYCKVEKQPRSKIDGFILNFGTFFQVIEAML------------NIHYAKKLYFPMLVVANVFLIIQQLFQWALIHEILQRKVDFFMKSRRTR-KIIFWKRYLNPCLVLWLISFGFLVAELINYVNTMDMLEKQKIEFWKIIRSVFIPFKILNRLHSFSVLIDI 459 The following BLAST results are available for this feature:
BLAST of SMED30030769 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030769 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030769 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030769 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030769 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030769 ID=SMED30030769|Name=SMED30030769|organism=Schmidtea mediterranea sexual|type=transcript|length=1589bpback to top protein sequence of SMED30030769-orf-1 >SMED30030769-orf-1 ID=SMED30030769-orf-1|Name=SMED30030769-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=525bp ESRALNVTTFKQKRLLIIKLLQNFEMAIHIEDHLIPVELIKSLKYFLLLLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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