Vacuolar protein sorting-associated protein 35

Overview
NameVacuolar protein sorting-associated protein 35
Smed IDSMED30030726
Length (bp)2620
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vacuolar protein sorting-associated protein 35 (SMED30030726) t-SNE clustered cells

Violin plots show distribution of expression levels for Vacuolar protein sorting-associated protein 35 (SMED30030726) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vacuolar protein sorting-associated protein 35 (SMED30030726) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vacuolar protein sorting-associated protein 35 (SMED30030726) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030726

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30030726SMESG000064165.1 dd_Smed_v4_2652_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30030726SMESG000064165.1 dd_Smed_v4_2652_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30030726SMESG000064165.1 dd_Smed_v4_2652_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030726SMESG000064165.1 dd_Smed_v4_2652_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30030726SMESG000064165.1 dd_Smed_v4_2652_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Human
Match: VPS35 (VPS35 retromer complex component [Source:HGNC Symbol;Acc:HGNC:13487])

HSP 1 Score: 815.839 bits (2106), Expect = 0.000e+0
Identity = 423/800 (52.88%), Postives = 588/800 (73.50%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ--GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE--VFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD    +G P  E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VL  +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + +++Q++S+   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R  D   DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S + E  ++  LI+
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD----EGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPD-QPVEDPDPEDFADEQSLVGRFIHLLRSEDP--DQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Celegans
Match: vps-35 (Vacuolar protein sorting-associated protein 35 [Source:UniProtKB/TrEMBL;Acc:A0A2C9C3A2])

HSP 1 Score: 572.778 bits (1475), Expect = 0.000e+0
Identity = 328/762 (43.04%), Postives = 488/762 (64.04%), Query Frame = 1 
Query:   70 EQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEF-QKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPD------------TDIEQGSP--ESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEEN-STGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECV-SLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLI 2304
            +QE+ LD+++  VK +SF+MKR LDK K MD LKHA  ML E+R  +LSPK YY LYM    EL+ LE  L++E+ Q+  K+ +LYE VQYA  I+PRLYLL+T+G V+IKC   SRK+IL+DLVEM RGVQHPLRGLFLRNYL+QC R  +LPD             ++ +G+P  +   GT  D+IDF+L+NF+EMNKLWVRMQHQG ++EKEKRE++R ELR+LVGTNL RL+QLE++  + Y   VLP++LEQIV+C D I+QEYLM+ VIQVF D+FHL TL   L A  +L+ +V+IK ++ AL++RL+ Y T  N     P  + LF+IFS++  ++++ R D+P +DI++L  SL+ LA+K YP++ +  +   +G    ++   ++ I     + REL +LL +PID YK++  + QL  + +++   +Y G+  I+ Y++ N L++  V   ++ ++S F  I  LL D  E  S D  + EEF +EQNLVA+ +HL R   D++D  + +L   +K LG GG  R   +  PI+F+   L  ++    DE + W  K+ K+F      + +L++    AEL +KL+L GA+T DR+  FE    + YEF S+A S+ E+++ DSR ++  + L   TL K   L EEN   L +Q   A A ++ KK DQ R++     L+W G+ LE  G+++++ KK++  L K+ K+A ECLE  ++ QL I+LLS + Y++  N  +++   I  LI
Sbjct:   10 DQEKFLDQSIRVVKAESFEMKRCLDKGKTMDALKHALQMLNEMRTAELSPKFYYRLYMDSMHELQCLEVNLVQEYAQEPAKLGNLYECVQYASAIIPRLYLLVTIGGVFIKCGLGSRKEILKDLVEMCRGVQHPLRGLFLRNYLMQCTR-SVLPDFPETEEMLVAHNDNLSKGTPKLKPRDGTVDDTIDFVLINFAEMNKLWVRMQHQGPSKEKEKREKDRMELRILVGTNLVRLAQLEALTEEMYVKDVLPSILEQIVSCRDPISQEYLMECVIQVFADDFHLATLTEFLNACGQLQQDVNIKILLIALVDRLALYTTSYNEGQPAPTKMQLFEIFSEQATTLIKNRPDMPLDDIVALHVSLVSLAVKCYPDRQDYANMTFQGLRQVIEEKGVTDIEAFGKVGRELTKLLNIPIDEYKNVLRLSQLPEYIKVMNYFDYRGQCNIASYMIQNMLEEETVFRNQDDVDSAFSLISSLLKDQ-EKQSSDSHETEEFADEQNLVARLLHLIRA--DDVDSQFLLLNSARKTLGEGGRHRLRYTLPPIIFELYRLVLQFSDMKDEDDKWDAKIRKMFVCAMGTIGALVSTAELAELPMKLYLNGAITADRVP-FEDNHTVVYEFVSKALSILEDDVVDSRDRVRCLHLTVGTLLKTTHLPEENWQPLANQTVLAAA-KMFKKPDQVRSLVTVAALYWHGQTLETNGEKMKNGKKVVDILRKAAKIARECLEPLVQQQLFIQLLSAYTYYYEDNCSEVNVDHIEELI 765
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Celegans
Match: vps-35 (Vacuolar protein sorting-associated protein 35 [Source:UniProtKB/TrEMBL;Acc:A0A2C9C3A2])

HSP 1 Score: 572.778 bits (1475), Expect = 0.000e+0
Identity = 328/762 (43.04%), Postives = 488/762 (64.04%), Query Frame = 1 
Query:   70 EQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEF-QKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPD------------TDIEQGSP--ESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEEN-STGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECV-SLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLI 2304
            +QE+ LD+++  VK +SF+MKR LDK K MD LKHA  ML E+R  +LSPK YY LYM    EL+ LE  L++E+ Q+  K+ +LYE VQYA  I+PRLYLL+T+G V+IKC   SRK+IL+DLVEM RGVQHPLRGLFLRNYL+QC R  +LPD             ++ +G+P  +   GT  D+IDF+L+NF+EMNKLWVRMQHQG ++EKEKRE++R ELR+LVGTNL RL+QLE++  + Y   VLP++LEQIV+C D I+QEYLM+ VIQVF D+FHL TL   L A  +L+ +V+IK ++ AL++RL+ Y T  N     P  + LF+IFS++  ++++ R D+P +DI++L  SL+ LA+K YP++ +  +   +G    ++   ++ I     + REL +LL +PID YK++  + QL  + +++   +Y G+  I+ Y++ N L++  V   ++ ++S F  I  LL D  E  S D  + EEF +EQNLVA+ +HL R   D++D  + +L   +K LG GG  R   +  PI+F+   L  ++    DE + W  K+ K+F      + +L++    AEL +KL+L GA+T DR+  FE    + YEF S+A S+ E+++ DSR ++  + L   TL K   L EEN   L +Q   A A ++ KK DQ R++     L+W G+ LE  G+++++ KK++  L K+ K+A ECLE  ++ QL I+LLS + Y++  N  +++   I  LI
Sbjct:   10 DQEKFLDQSIRVVKAESFEMKRCLDKGKTMDALKHALQMLNEMRTAELSPKFYYRLYMDSMHELQCLEVNLVQEYAQEPAKLGNLYECVQYASAIIPRLYLLVTIGGVFIKCGLGSRKEILKDLVEMCRGVQHPLRGLFLRNYLMQCTR-SVLPDFPETEEMLVAHNDNLSKGTPKLKPRDGTVDDTIDFVLINFAEMNKLWVRMQHQGPSKEKEKREKDRMELRILVGTNLVRLAQLEALTEEMYVKDVLPSILEQIVSCRDPISQEYLMECVIQVFADDFHLATLTEFLNACGQLQQDVNIKILLIALVDRLALYTTSYNEGQPAPTKMQLFEIFSEQATTLIKNRPDMPLDDIVALHVSLVSLAVKCYPDRQDYANMTFQGLRQVIEEKGVTDIEAFGKVGRELTKLLNIPIDEYKNVLRLSQLPEYIKVMNYFDYRGQCNIASYMIQNMLEEETVFRNQDDVDSAFSLISSLLKDQ-EKQSSDSHETEEFADEQNLVARLLHLIRA--DDVDSQFLLLNSARKTLGEGGRHRLRYTLPPIIFELYRLVLQFSDMKDEDDKWDAKIRKMFVCAMGTIGALVSTAELAELPMKLYLNGAITADRVP-FEDNHTVVYEFVSKALSILEDDVVDSRDRVRCLHLTVGTLLKTTHLPEENWQPLANQTVLAAA-KMFKKPDQVRSLVTVAALYWHGQTLETNGEKMKNGKKVVDILRKAAKIARECLEPLVQQQLFIQLLSAYTYYYEDNCSEVNVDHIEELI 765
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Celegans
Match: vps-35 (Vacuolar protein sorting-associated protein 35 [Source:UniProtKB/TrEMBL;Acc:A0A2C9C3A2])

HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0
Identity = 320/743 (43.07%), Postives = 473/743 (63.66%), Query Frame = 1 
Query:  127 MKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEF-QKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPD------------TDIEQGSP--ESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEEN-STGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECV-SLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLI 2304
            MKR LDK K MD LKHA  ML E+R  +LSPK YY LYM    EL+ LE  L++E+ Q+  K+ +LYE VQYA  I+PRLYLL+T+G V+IKC   SRK+IL+DLVEM RGVQHPLRGLFLRNYL+QC R  +LPD             ++ +G+P  +   GT  D+IDF+L+NF+EMNKLWVRMQHQG ++EKEKRE++R ELR+LVGTNL RL+QLE++  + Y   VLP++LEQIV+C D I+QEYLM+ VIQVF D+FHL TL   L A  +L+ +V+IK ++ AL++RL+ Y T  N     P  + LF+IFS++  ++++ R D+P +DI++L  SL+ LA+K YP++ +  +   +G    ++   ++ I     + REL +LL +PID YK++  + QL  + +++   +Y G+  I+ Y++ N L++  V   ++ ++S F  I  LL D  E  S D  + EEF +EQNLVA+ +HL R   D++D  + +L   +K LG GG  R   +  PI+F+   L  ++    DE + W  K+ K+F      + +L++    AEL +KL+L GA+T DR+  FE    + YEF S+A S+ E+++ DSR ++  + L   TL K   L EEN   L +Q   A A ++ KK DQ R++     L+W G+ LE  G+++++ KK++  L K+ K+A ECLE  ++ QL I+LLS + Y++  N  +++   I  LI
Sbjct:    1 MKRCLDKGKTMDALKHALQMLNEMRTAELSPKFYYRLYMDSMHELQCLEVNLVQEYAQEPAKLGNLYECVQYASAIIPRLYLLVTIGGVFIKCGLGSRKEILKDLVEMCRGVQHPLRGLFLRNYLMQCTR-SVLPDFPETEEMLVAHNDNLSKGTPKLKPRDGTVDDTIDFVLINFAEMNKLWVRMQHQGPSKEKEKREKDRMELRILVGTNLVRLAQLEALTEEMYVKDVLPSILEQIVSCRDPISQEYLMECVIQVFADDFHLATLTEFLNACGQLQQDVNIKILLIALVDRLALYTTSYNEGQPAPTKMQLFEIFSEQATTLIKNRPDMPLDDIVALHVSLVSLAVKCYPDRQDYANMTFQGLRQVIEEKGVTDIEAFGKVGRELTKLLNIPIDEYKNVLRLSQLPEYIKVMNYFDYRGQCNIASYMIQNMLEEETVFRNQDDVDSAFSLISSLLKDQ-EKQSSDSHETEEFADEQNLVARLLHLIRA--DDVDSQFLLLNSARKTLGEGGRHRLRYTLPPIIFELYRLVLQFSDMKDEDDKWDAKIRKMFVCAMGTIGALVSTAELAELPMKLYLNGAITADRVP-FEDNHTVVYEFVSKALSILEDDVVDSRDRVRCLHLTVGTLLKTTHLPEENWQPLANQTVLAAA-KMFKKPDQVRSLVTVAALYWHGQTLETNGEKMKNGKKVVDILRKAAKIARECLEPLVQQQLFIQLLSAYTYYYEDNCSEVNVDHIEELI 737
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Celegans
Match: vps-35 (Vacuolar protein sorting-associated protein 35 [Source:UniProtKB/TrEMBL;Acc:A0A2C9C3A2])

HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0
Identity = 320/743 (43.07%), Postives = 473/743 (63.66%), Query Frame = 1 
Query:  127 MKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEF-QKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPD------------TDIEQGSP--ESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEEN-STGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECV-SLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLI 2304
            MKR LDK K MD LKHA  ML E+R  +LSPK YY LYM    EL+ LE  L++E+ Q+  K+ +LYE VQYA  I+PRLYLL+T+G V+IKC   SRK+IL+DLVEM RGVQHPLRGLFLRNYL+QC R  +LPD             ++ +G+P  +   GT  D+IDF+L+NF+EMNKLWVRMQHQG ++EKEKRE++R ELR+LVGTNL RL+QLE++  + Y   VLP++LEQIV+C D I+QEYLM+ VIQVF D+FHL TL   L A  +L+ +V+IK ++ AL++RL+ Y T  N     P  + LF+IFS++  ++++ R D+P +DI++L  SL+ LA+K YP++ +  +   +G    ++   ++ I     + REL +LL +PID YK++  + QL  + +++   +Y G+  I+ Y++ N L++  V   ++ ++S F  I  LL D  E  S D  + EEF +EQNLVA+ +HL R   D++D  + +L   +K LG GG  R   +  PI+F+   L  ++    DE + W  K+ K+F      + +L++    AEL +KL+L GA+T DR+  FE    + YEF S+A S+ E+++ DSR ++  + L   TL K   L EEN   L +Q   A A ++ KK DQ R++     L+W G+ LE  G+++++ KK++  L K+ K+A ECLE  ++ QL I+LLS + Y++  N  +++   I  LI
Sbjct:    1 MKRCLDKGKTMDALKHALQMLNEMRTAELSPKFYYRLYMDSMHELQCLEVNLVQEYAQEPAKLGNLYECVQYASAIIPRLYLLVTIGGVFIKCGLGSRKEILKDLVEMCRGVQHPLRGLFLRNYLMQCTR-SVLPDFPETEEMLVAHNDNLSKGTPKLKPRDGTVDDTIDFVLINFAEMNKLWVRMQHQGPSKEKEKREKDRMELRILVGTNLVRLAQLEALTEEMYVKDVLPSILEQIVSCRDPISQEYLMECVIQVFADDFHLATLTEFLNACGQLQQDVNIKILLIALVDRLALYTTSYNEGQPAPTKMQLFEIFSEQATTLIKNRPDMPLDDIVALHVSLVSLAVKCYPDRQDYANMTFQGLRQVIEEKGVTDIEAFGKVGRELTKLLNIPIDEYKNVLRLSQLPEYIKVMNYFDYRGQCNIASYMIQNMLEEETVFRNQDDVDSAFSLISSLLKDQ-EKQSSDSHETEEFADEQNLVARLLHLIRA--DDVDSQFLLLNSARKTLGEGGRHRLRYTLPPIIFELYRLVLQFSDMKDEDDKWDAKIRKMFVCAMGTIGALVSTAELAELPMKLYLNGAITADRVP-FEDNHTVVYEFVSKALSILEDDVVDSRDRVRCLHLTVGTLLKTTHLPEENWQPLANQTVLAAA-KMFKKPDQVRSLVTVAALYWHGQTLETNGEKMKNGKKVVDILRKAAKIARECLEPLVQQQLFIQLLSAYTYYYEDNCSEVNVDHIEELI 737
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Fly
Match: Vps35 (gene:FBgn0034708 transcript:FBtr0071771)

HSP 1 Score: 680.248 bits (1754), Expect = 0.000e+0
Identity = 375/797 (47.05%), Postives = 529/797 (66.37%), Query Frame = 1 
Query:   67 DEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTG-------IPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYD------SPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSAD 2418
            D+QE+LL EA+   + Q+FQM   LDK +++D LK A+ MLGELR   LSPKSYYELYMA+T+EL HLE YL E+  K    +DLYELVQY+  IVPRLYLLITVG+VYIK +   ++ IL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD  +E+   E   G   D+IDF+L NF+EMNKLWVRMQHQGH+ EK +RE+ER EL++LVGTNL RLSQLES  ++ Y+  +LP +LEQ+V+C DAIAQEYLM+ +IQVFPDEFHL+TL+  L++ ++LE+ V++K+II +LIERL+ Y      T        IP  + LF++FS +V ++++ R D+P ED +SL+ +LL LA KVYP++++  DKV+      L+ MN++ IS+   +++EL RLL++ ID Y +   I+QL+NF  L++  +Y  RK ++ YL+ N LD   ++ T +Q +SL   I  L+ D      +         D EEF EEQ +VA+ IHL R   D  D  YK+L   +K LG GG +R      P+VF    LA +Y    ++ E W KK +K+ +  H  +S +A    A+LAL+L+LQGAL I  I  +   E +AYEF +QAFSL+E+EI+DS+ Q+ AITLI ST E+M C  EEN   LR+ CA A + +L+KK DQ R V     LFWSGK   + G+E+RD K+ + CL K  ++A++CL+  ++ QL +ELL+ +L++F   +  I+ A +N LI  + + LP+++   +   I  H+ N + +I  + +S D
Sbjct:   27 DDQEKLLAEAIGLARKQAFQMNHFLDKERMLDSLKCASTMLGELRTSVLSPKSYYELYMAVTNELCHLELYLSEKIDKK---TDLYELVQYSHTIVPRLYLLITVGIVYIKNDPTLKRSILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDVMVEENEHE---GNVYDAIDFVLTNFAEMNKLWVRMQHQGHSSEKTRREKEREELKILVGTNLVRLSQLESATLEIYQRLILPGILEQVVSCRDAIAQEYLMECIIQVFPDEFHLKTLDPFLKSCAQLETGVNVKNIIISLIERLAAYNQRSGKTSGNAIDAIIPAEVELFEVFSVQVANIVQTRMDMPLEDTISLQVALLSLAQKVYPDRVDYVDKVLGTTAQILQRMNMNNISHLLSVNQELSRLLRICIDFYNNALTIIQLQNFCPLLEKFDYTSRKSLALYLVMNILDNETLVPTADQADSLLTIITPLIKDDDTNKENGAAAGNTTPDAEEFAEEQGVVARFIHLMR--SDEPDMQYKMLQTARKHLGNGGGQRLKHVLPPLVFAAYQLAFKYKAIAEQDENWDKKCQKIVQYCHSTISALAKADLADLALRLYLQGALVIGEIG-YTNHETVAYEFMTQAFSLYEDEISDSKAQLAAITLIMSTFEQMSCFGEENAEPLRTNCALAAS-KLLKKPDQCRGVVACAALFWSGK---QNGEEMRDEKRTLDCLKKGARIASQCLDTGVQVQLYVELLNHYLFYFERGNSLITVAMLNQLIAKVNEELPNLEPSEETKQIESHYKNTLAHIRSRMESND 809
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Fly
Match: Vps35 (gene:FBgn0034708 transcript:FBtr0071772)

HSP 1 Score: 680.248 bits (1754), Expect = 0.000e+0
Identity = 375/797 (47.05%), Postives = 529/797 (66.37%), Query Frame = 1 
Query:   67 DEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTG-------IPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYD------SPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSAD 2418
            D+QE+LL EA+   + Q+FQM   LDK +++D LK A+ MLGELR   LSPKSYYELYMA+T+EL HLE YL E+  K    +DLYELVQY+  IVPRLYLLITVG+VYIK +   ++ IL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD  +E+   E   G   D+IDF+L NF+EMNKLWVRMQHQGH+ EK +RE+ER EL++LVGTNL RLSQLES  ++ Y+  +LP +LEQ+V+C DAIAQEYLM+ +IQVFPDEFHL+TL+  L++ ++LE+ V++K+II +LIERL+ Y      T        IP  + LF++FS +V ++++ R D+P ED +SL+ +LL LA KVYP++++  DKV+      L+ MN++ IS+   +++EL RLL++ ID Y +   I+QL+NF  L++  +Y  RK ++ YL+ N LD   ++ T +Q +SL   I  L+ D      +         D EEF EEQ +VA+ IHL R   D  D  YK+L   +K LG GG +R      P+VF    LA +Y    ++ E W KK +K+ +  H  +S +A    A+LAL+L+LQGAL I  I  +   E +AYEF +QAFSL+E+EI+DS+ Q+ AITLI ST E+M C  EEN   LR+ CA A + +L+KK DQ R V     LFWSGK   + G+E+RD K+ + CL K  ++A++CL+  ++ QL +ELL+ +L++F   +  I+ A +N LI  + + LP+++   +   I  H+ N + +I  + +S D
Sbjct:    8 DDQEKLLAEAIGLARKQAFQMNHFLDKERMLDSLKCASTMLGELRTSVLSPKSYYELYMAVTNELCHLELYLSEKIDKK---TDLYELVQYSHTIVPRLYLLITVGIVYIKNDPTLKRSILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDVMVEENEHE---GNVYDAIDFVLTNFAEMNKLWVRMQHQGHSSEKTRREKEREELKILVGTNLVRLSQLESATLEIYQRLILPGILEQVVSCRDAIAQEYLMECIIQVFPDEFHLKTLDPFLKSCAQLETGVNVKNIIISLIERLAAYNQRSGKTSGNAIDAIIPAEVELFEVFSVQVANIVQTRMDMPLEDTISLQVALLSLAQKVYPDRVDYVDKVLGTTAQILQRMNMNNISHLLSVNQELSRLLRICIDFYNNALTIIQLQNFCPLLEKFDYTSRKSLALYLVMNILDNETLVPTADQADSLLTIITPLIKDDDTNKENGAAAGNTTPDAEEFAEEQGVVARFIHLMR--SDEPDMQYKMLQTARKHLGNGGGQRLKHVLPPLVFAAYQLAFKYKAIAEQDENWDKKCQKIVQYCHSTISALAKADLADLALRLYLQGALVIGEIG-YTNHETVAYEFMTQAFSLYEDEISDSKAQLAAITLIMSTFEQMSCFGEENAEPLRTNCALAAS-KLLKKPDQCRGVVACAALFWSGK---QNGEEMRDEKRTLDCLKKGARIASQCLDTGVQVQLYVELLNHYLFYFERGNSLITVAMLNQLIAKVNEELPNLEPSEETKQIESHYKNTLAHIRSRMESND 790
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Zebrafish
Match: vps35 (VPS35 retromer complex component [Source:ZFIN;Acc:ZDB-GENE-030131-2042])

HSP 1 Score: 810.831 bits (2093), Expect = 0.000e+0
Identity = 417/789 (52.85%), Postives = 582/789 (73.76%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE 2424
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY++    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD D EQ   E   G   DSIDF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V++YK  VL  VLEQ+VNC D++AQEYLM+ +IQVFPDEFHL+TL   LR+ +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +LQL++F  L +  +Y  RK +SCY+L+N LD N ++  ++Q++++   +  L+ D P+    ++ DPE+F EEQ+LV + IHL     D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+  C SEENH  LR+QCA A + +L+KK DQ RAV++  HLFWSG+  ++ G E+RD ++++ CL K+ K+AN+C++ +++ QL IE+L+R++ F+   ++ ++   +N LI+ IR+ LP++++  + + IN HF N + ++ ++++S + E
Sbjct:   42 SPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD-DGEQAE-EEMTGDINDSIDFVLLNFAEMNKLWVRMQHQGHSRDREKREKERQELRILVGTNLVRLSQLEGVNVEKYKQIVLSGVLEQVVNCRDSLAQEYLMECIIQVFPDEFHLQTLNPFLRSCAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLESTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLQLKHFPPLFEYFDYESRKSMSCYVLSNILDYNTIIVAQDQVDAILNLVSTLIQDQPD-QPAEDPDPEDFAEEQSLVGRFIHLLH--SDDPDQQYLILNTARKHFGAGGNQRIRHTLPPLVFAAYQLAFRYKENSSSDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERTRCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVSICAHLFWSGRNTDKGGDEIRDGRRVMECLKKALKIANQCMDPSLQVQLFIEILNRYICFYERENDAVTVQVLNQLIQKIREDLPNLEASEETEQINKHFHNTLEHLRLQRESPESE 822
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Zebrafish
Match: vps35 (VPS35 retromer complex component [Source:ZFIN;Acc:ZDB-GENE-030131-2042])

HSP 1 Score: 254.988 bits (650), Expect = 6.630e-79
Identity = 127/167 (76.05%), Postives = 143/167 (85.63%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPE 558
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY++    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD D EQ   E
Sbjct:   30 SPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD-DGEQAEEE 194
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Xenopus
Match: vps35 (VPS35 retromer complex component [Source:NCBI gene;Acc:549980])

HSP 1 Score: 812.372 bits (2097), Expect = 0.000e+0
Identity = 419/798 (52.51%), Postives = 585/798 (73.31%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKS--ADDEVFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+D+VEM RGVQHPLRGLFLRNYLLQC R ++LPD    + + E   G   DS+DF+LLNF+EMNKLWVRMQHQGH+R+KEKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VLP +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N L+ N  + ++EQ++++   +  L+ D P+    +E DPE+F +EQ LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T EKM C SEENH  LR+Q A A + +L+KK DQ RAV+++ HLFWSG+  ++ G+E+   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP++ +  + D I+ HF N + ++ +++ S   D  ++  L++
Sbjct:    7 SPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFSQSRKDILKDMVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDEG--EQTNEEVTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDKEKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNALEYNTEIGSQEQVDAIMNLVSTLIQDQPD-QPAEEPDPEDFADEQGLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYKLAFRYKENAKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFEKMKCFSEENHEPLRTQSALAAS-KLLKKPDQCRAVSISAHLFWSGRNTDKNGEEIHSGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDGVTVQVLNQLIQKIREDLPNLDATEETDQISTHFKNTLEHLRLRKDSPEGDGPIYEGLVL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Xenopus
Match: vps35 (VPS35 retromer complex component [Source:NCBI gene;Acc:549980])

HSP 1 Score: 775.007 bits (2000), Expect = 0.000e+0
Identity = 411/798 (51.50%), Postives = 567/798 (71.05%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKS--ADDEVFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+D+VEM RGVQHPLRGLFLRNYLLQC R ++LPD    + + E   G   DS+DF+LLNF+EMNKLWVRMQHQGH+R+KEKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VLP +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K                      +  +  S     S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N L+ N  + ++EQ++++   +  L+ D P+    +E DPE+F +EQ LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T EKM C SEENH  LR+Q A A + +L+KK DQ RAV+++ HLFWSG+  ++ G+E+   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP++ +  + D I+ HF N + ++ +++ S   D  ++  L++
Sbjct:    7 SPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFSQSRKDILKDMVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDEG--EQTNEEVTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDKEKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMK----------------------LCHTGTSRPVLASKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNALEYNTEIGSQEQVDAIMNLVSTLIQDQPD-QPAEEPDPEDFADEQGLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYKLAFRYKENAKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFEKMKCFSEENHEPLRTQSALAAS-KLLKKPDQCRAVSISAHLFWSGRNTDKNGEEIHSGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDGVTVQVLNQLIQKIREDLPNLDATEETDQISTHFKNTLEHLRLRKDSPEGDGPIYEGLVL 774
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Xenopus
Match: vps35 (VPS35 retromer complex component [Source:NCBI gene;Acc:549980])

HSP 1 Score: 734.561 bits (1895), Expect = 0.000e+0
Identity = 391/770 (50.78%), Postives = 543/770 (70.52%), Query Frame = 1 
Query:  142 DKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKS--ADDEVFSQLII 2445
            DK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+D+VEM RGVQHPLRGLFLRNYLLQC R ++LPD    + + E   G   DS+DF+LLNF+EMNKLWVRMQHQGH+R+KEKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VLP +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K                      +  +  S     S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N L+ N  + ++EQ++++   +  L+ D P+    +E DPE+F +EQ LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T EKM C SEENH  LR+Q A A + +L+KK DQ RAV+++ HLFWSG+  ++ G+E+   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP++ +  + D I+ HF N + ++ +++ S   D  ++  L++
Sbjct:    3 DKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFSQSRKDILKDMVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDEG--EQTNEEVTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDKEKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMK----------------------LCHTGTSRPVLASKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNALEYNTEIGSQEQVDAIMNLVSTLIQDQPD-QPAEEPDPEDFADEQGLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYKLAFRYKENAKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFEKMKCFSEENHEPLRTQSALAAS-KLLKKPDQCRAVSISAHLFWSGRNTDKNGEEIHSGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDGVTVQVLNQLIQKIREDLPNLDATEETDQISTHFKNTLEHLRLRKDSPEGDGPIYEGLVL 742
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Mouse
Match: Vps35 (VPS35 retromer complex component [Source:MGI Symbol;Acc:MGI:1890467])

HSP 1 Score: 816.224 bits (2107), Expect = 0.000e+0
Identity = 423/800 (52.88%), Postives = 588/800 (73.50%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ--GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE--VFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD    +G P  E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VL  +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + +++Q++S+   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP+++S  + + IN HF N + ++  +++S + E  ++  LI+
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD----EGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPD-QPVEDPDPEDFADEQSLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRSRRESPESEGPIYEGLIL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. UniProt/SwissProt
Match: sp|Q2HJG5|VPS35_BOVIN (Vacuolar protein sorting-associated protein 35 OS=Bos taurus OX=9913 GN=VPS35 PE=2 SV=1)

HSP 1 Score: 816.609 bits (2108), Expect = 0.000e+0
Identity = 424/800 (53.00%), Postives = 588/800 (73.50%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ--GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE--VFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD    +G P  E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VL  +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + +++Q++S+   +  L+ D P+    +E DPE+F +EQ+LV + IHL R  D   DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S + E  ++  LI+
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD----EGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPTDIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPD-QPVEEPDPEDFADEQSLVGRFIHLLRSEDP--DQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. UniProt/SwissProt
Match: sp|Q9EQH3|VPS35_MOUSE (Vacuolar protein sorting-associated protein 35 OS=Mus musculus OX=10090 GN=Vps35 PE=1 SV=1)

HSP 1 Score: 816.224 bits (2107), Expect = 0.000e+0
Identity = 423/800 (52.88%), Postives = 588/800 (73.50%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ--GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE--VFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD    +G P  E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VL  +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + +++Q++S+   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP+++S  + + IN HF N + ++  +++S + E  ++  LI+
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD----EGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPD-QPVEDPDPEDFADEQSLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRSRRESPESEGPIYEGLIL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. UniProt/SwissProt
Match: sp|Q96QK1|VPS35_HUMAN (Vacuolar protein sorting-associated protein 35 OS=Homo sapiens OX=9606 GN=VPS35 PE=1 SV=2)

HSP 1 Score: 815.839 bits (2106), Expect = 0.000e+0
Identity = 423/800 (52.88%), Postives = 588/800 (73.50%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ--GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE--VFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD    +G P  E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VL  +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + +++Q++S+   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R  D   DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   ++ ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S + E  ++  LI+
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD----EGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPD-QPVEDPDPEDFADEQSLVGRFIHLLRSEDP--DQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. UniProt/SwissProt
Match: sp|Q54C24|VPS35_DICDI (Vacuolar protein sorting-associated protein 35 OS=Dictyostelium discoideum OX=44689 GN=vps35 PE=3 SV=1)

HSP 1 Score: 508.064 bits (1307), Expect = 4.956e-167
Identity = 308/788 (39.09%), Postives = 481/788 (61.04%), Query Frame = 1 
Query:   55 MATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEI------WQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKS-EKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIE 2397
            ++ ++EQ +  +EA   V +Q   MK SLD SKLMD LK+A+N++ ELR   LSPKSYY LY+   D L++L +YL EE + G+K+ +LYE+VQ+AGN++PRLYLLITVG VYIK  +   KD+L+DL+EM RGVQHP RGLFLR+YL +  + D LPD D        E GT  DSIDFI+ NF+E NKLWVRMQHQ  T+++E+RE ER ELRLLVG NL+RL+QL+ +D + Y   VLP V+EQI+NC D IAQ+YLM+++IQVFPDEFHL TL+ +L+  ++L+S V +K+II +LI+RL+ +AT  N+  +PD+I +FDIF   V  +++ R ++  +DI+ L  SLL+L LK YP   +  ++V+      + N     I+  +C+ +++I+LL++P+DV+K++ ++L+L N+  LI  L YN RK +S  ++ NT++ + ++   E + +L + IQ L+ D  +    D++D E+F+EEQN VA  IHLF   D   ++ +KI I  +   G GG  R   + +P+VF +L     +  + D   I      W     K+F    E +  +A+   A+L+ +L+LQ   T D      + + LA     +A  +F+E+IAD + Q+ A+ L+ STL  +   +EE + +L +Q  K  + RL+   DQ++ ++   HLFW   P  +Y    ++   ++  L K+   ++NE   G       +++L+  L++    ++ +    ++ L++ IR          + D    +  N I YI+
Sbjct:   13 LSPEEEQAKFFEEAKNNVMIQGHHMKLSLDNSKLMDALKYASNIINELRTSLLSPKSYYALYLVAFDYLQYLNTYLYEE-KHGKKMIELYEVVQHAGNVLPRLYLLITVGSVYIKTKQAPAKDVLKDLIEMCRGVQHPTRGLFLRHYLSEVTK-DKLPDID-----SSVENGTVMDSIDFIIQNFTETNKLWVRMQHQAPTKDRERRENERLELRLLVGKNLSRLAQLDGVDQKTYSEVVLPKVVEQIINCKDKIAQQYLMEILIQVFPDEFHLATLDIILQTCAQLQSGVDVKTIIASLIDRLANFAT-RNADLVPDNIKIFDIFFNNVKEIIQARPNMELQDILGLHVSLLNLTLKCYPTNKDNANEVLGLCQSIIVNKAKEDINKPTCV-KQIIQLLQIPLDVFKNVLVVLKLSNYQPLISCLSYNNRKKVSLDIVNNTINNSTIIEEPEAVNNLLETIQTLIKDEQDQPDMDDIDKEDFQEEQNKVASLIHLFDSEDP--EKLFKIYIIARGHFGKGGPHRIRHTLVPLVFCSLRFIRNFKQQVDSGVISLDENKWIAIGSKIFTFVSETIKALADIKLADLSFRLYLQALQTFDHCGLVSRVKELAI----KALLIFQEDIADFKAQVMALQLLISTLNSLSIPNEEIYESLAAQTIKQAS-RLLLPQDQAKLISTCSHLFWVDNPSRQY----QNPDSVLQALKKALSIISNESSPGLGT---FVDILNECLFYCDKETDAVPIQFVSDLVELIRT-----THVKEADPALPYLQNTIKYIQ 772
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. UniProt/SwissProt
Match: sp|Q7X659|VP35A_ARATH (Vacuolar protein sorting-associated protein 35A OS=Arabidopsis thaliana OX=3702 GN=VPS35A PE=1 SV=1)

HSP 1 Score: 497.664 bits (1280), Expect = 6.139e-163
Identity = 311/761 (40.87%), Postives = 472/761 (62.02%), Query Frame = 1 
Query:   67 DEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPD--SIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKM--EKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLE-------GTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIR 2316
            +++E+ L       K  +F M+R++D + L D LK++  ML ELR  +LSP  YY+LYM   DELR LE + +EE ++G  V +LYELVQ+AGNI+PRLYLL T G VYIK  E   K+IL+DLVEM RG+QHPLRGLFLR+YL Q  R D LPD   E    E +  T  D+++F+LLNF+EMNKLWVRMQHQG  REKE+RE+ER ELR LVG NL+ LSQLE +D+  Y+ TVLP VLEQIVNC D IAQ YL+D +IQVFPDE+HL+TL+ LL A  +L+++V I +++  L+ERLS YA   N+  +P    +  F   +  +  ++E ++D+P    ++L +SLL   L V+P++L+  D+V+   +  L      KI  +   ++EL+ LL  P++ Y D+   L+L N+  +++ L+   +++++  ++ + +  N ++ T E++E+LF+ I+ ++ D  E     EVD ++F+EEQN VA  IH+    +D+ ++ +KI+  +KK    GG KR   +  P+V  TL L  R     D     +  +   K+F+  ++ +  + N  + +LA +L+LQ A   D+  +    E +AYEFF+QA+ L+EEEI+DS+ Q+ A+ LI  TL++M     EN   L  + A   A +L+KK DQ RAV    HLFW    LE+  + ++D ++++ CL ++ K+AN   +        T    L IE+L+++LYF+     +I+   + SLIK I+
Sbjct:    7 EDEEKWLAAGAAAFKQNAFYMQRAIDSNNLKDALKYSAQMLSELRTSKLSPHKYYDLYMRAFDELRKLEIFFMEETRRGCSVIELYELVQHAGNILPRLYLLCTAGSVYIKTKEAPAKEILKDLVEMCRGIQHPLRGLFLRSYLAQISR-DKLPDIGSEY---EGDADTVIDAVEFVLLNFTEMNKLWVRMQHQGPAREKERREKERGELRDLVGKNLHVLSQLEGVDLDMYRDTVLPRVLEQIVNCRDEIAQYYLIDCIIQVFPDEYHLQTLDVLLGACPQLQASVDIMTVLSRLMERLSNYAA-LNAEVLPYFLQVEAFSKLNNAIGKVIEAQEDMPILSAVTLYSSLLKFTLHVHPDRLDYADQVLGSCVKQLSGKG--KIDDTRA-TKELVSLLSAPLEKYNDVVTALKLTNYPLVVEYLDTETKRIMATVIVRSIMKNNTLITTAEKVEALFELIKGIINDLDEPQG-LEVDEDDFQEEQNSVALLIHMLY--NDDPEEMFKIVNVLKKHFLTGGPKRLKFTIPPLVVSTLKLIRRLPVEGDNPFGKEASVTATKIFQFLNQIIEALPNVPSPDLAFRLYLQCAEAADKCDE----EPIAYEFFTQAYILYEEEISDSKAQVTALQLIIGTLQRMQVFGVENRDTLTHK-ATGYAAKLLKKPDQCRAVYACSHLFW----LEDR-ETIQDGERVLLCLKRALKIANSAQQVANTARGSTGSVTLFIEILNKYLYFYEKGVPQITVESVESLIKLIK 746
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. TrEMBL
Match: A0A226NJQ2 (Vacuolar protein sorting-associated protein 35 OS=Callipepla squamata OX=9009 GN=ASZ78_010346 PE=3 SV=1)

HSP 1 Score: 821.231 bits (2120), Expect = 0.000e+0
Identity = 424/789 (53.74%), Postives = 584/789 (74.02%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE 2424
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+N LGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD   EQ   E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VLP +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + ++EQ++++   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R   D+ DQ YKIL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K++I CL K+ K+AN+C++ +++ QL IE+L+R++YF+   +E ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S + E
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNKLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDEG-EQADEET-TGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLRLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQEQVDAIMNLVSTLIQDQPD-QPAEDPDPEDFADEQSLVGRFIHLLR--SDDPDQQYKILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVIECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENEAVTIQVLNQLIQKIREDLPNLESTEETEQINKHFHNTLEHLRLRRESPESE 787
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. TrEMBL
Match: A0A091P082 (Vacuolar protein sorting-associated protein 35 (Fragment) OS=Haliaeetus albicilla OX=8969 GN=N329_01593 PE=4 SV=1)

HSP 1 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 426/798 (53.38%), Postives = 589/798 (73.81%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE--VFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD   EQ   E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VLP +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+PID Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD N  + ++EQ++++   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   +E ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S + E  ++  L+I
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPDEG-EQADEET-TGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPIDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQEQVDAIMNLVSTLIQDQPD-QPAEDPDPEDFADEQSLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENEAVTIQVLNQLIQKIREDLPNLESTEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLVI 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. TrEMBL
Match: K9IZN2 (Vacuolar protein sorting-associated protein 35 OS=Desmodus rotundus OX=9430 PE=2 SV=1)

HSP 1 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 424/791 (53.60%), Postives = 580/791 (73.32%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ--GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE 2424
            + QDEQE+LLDEA   VK+QSFQMKR LDKSKLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY+K    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R + LPD    +G P  E+  G   DS+DF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V+RYK  VL  +LEQ+VNC DA+AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+PPED++SL+ SL++LA+K YP +++  DKV++  +D    +N+  I+ SS +S+EL RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+N LD +  + +++Q++S+   +  L+ D P+    ++ DPE+F +EQ+LV + IHL R  D   DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  +  G+EL   K+++ CL K+ K+AN+C++ +++ QL IE+L+R++YF+   S+ ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S + E
Sbjct:    7 SPQDEQEKLLDEATQAVKVQSFQMKRCLDKSKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NTLPD----EGEPADEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPPEDVVSLQVSLINLAMKCYPERVDYVDKVLETTVDIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDHSTEVVSQDQVDSIMNLVSTLIQDQPD-QPVEDPDPEDFADEQSLVGRFIHLLRSEDP--DQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKQNSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFISQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAS-KLLKKPDQGRAVSTCAHLFWSGRNTDRNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKESDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESE 787
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. TrEMBL
Match: A0A3B4DYB9 (Vacuolar protein sorting-associated protein 35 OS=Pygocentrus nattereri OX=42514 PE=3 SV=1)

HSP 1 Score: 819.691 bits (2116), Expect = 0.000e+0
Identity = 423/789 (53.61%), Postives = 583/789 (73.89%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE 2424
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY++    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD D EQ   E   G   DSIDF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V++YK  VLP VLEQ+VNC D++AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP +++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +LQL++F  L +  +Y  RK +SCY+L+NTLD N  +  +EQ++++   +  L+ D P+    ++ DPE+F EEQ+LV + IHL    D   DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV++  HLFWSG+  ++ G+E+RD K+++ CL K+ K+AN+C++ +++ QL IE+L+R++ F+   ++ ++   +N LI+ IR+ LP++++  + + IN HF N + ++ ++++S + E
Sbjct:    7 SPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD-DGEQAEGEM-TGDINDSIDFVLLNFAEMNKLWVRMQHQGHSRDREKREKERQELRILVGTNLVRLSQLEGVNVEKYKQIVLPGVLEQVVNCRDSLAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPERVDYVDKVLESTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLQLKHFPPLFEYFDYESRKSMSCYVLSNTLDYNTTIVAQEQVDAILNLVSTLIQDQPD-QPAEDPDPEDFAEEQSLVGRFIHLLHSEDP--DQQYLILNTARKHFGAGGNQRIRYTLPPLVFAAYQLAFRYKENSSSDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMRCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVSICAHLFWSGRNTDKNGEEIRDGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYVCFYERENDAVTVQVLNQLIQKIREDLPNLEASEETEQINKHFHNTLEHLRLQRESPESE 787
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. TrEMBL
Match: A0A3B3RM19 (Vacuolar protein sorting-associated protein 35 OS=Paramormyrops kingsleyae OX=1676925 PE=3 SV=1)

HSP 1 Score: 819.691 bits (2116), Expect = 0.000e+0
Identity = 427/799 (53.44%), Postives = 591/799 (73.97%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQ-GTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKS--ADDEVFSQLII 2445
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY++    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD D EQ  PE E  G   DSIDF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V++YK  VL  VLEQ+VNC D++AQEYLM+ +IQVFPDEFHL+TL   LR+ +EL  +V++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL+RLLK+P+D Y +I  +L+L++F  L +  +Y  RK +SCY+L+NTLD    +  +EQ++++   +  L+ D P+    ++ DPE+F EEQ+LV + IHL R   D+ DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+  C SEENH  LR+QCA A + +L+KK DQ RAV+   HLFWSG+  ++ G+E+RD K+++ CL K+ K+AN+C++ +++ QL IE+L+R+LYF+   +E ++   +N LI+ IR+ LP+++S  + + IN HF N + ++ ++++S  AD  V+  L++
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD-DGEQ--PEEETTGDINDSIDFVLLNFAEMNKLWVRMQHQGHSRDREKREKERQELRILVGTNLVRLSQLEGVNVEKYKQVVLTGVLEQVVNCRDSLAQEYLMECIIQVFPDEFHLQTLNPFLRSCAELHQHVNVKNIIIALIDRLALFAHREDGPGIPTDIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLESTVEIFNKLNLEHIATSSAVSKELMRLLKIPVDTYNNILTVLKLKHFHLLFEYFDYESRKTMSCYVLSNTLDYGTEVVAQEQVDAILNLVSTLIQDQPD-QPAEDPDPEDFAEEQSLVGRFIHLLR--SDDPDQQYLILNTARKHFGAGGNQRIRYTLPPLVFAAYQLAFRYKENSKSDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIVGTFERTRCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVSTCAHLFWSGRNTDKNGEEIRDGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYLYFYGKENEAVTIQVLNQLIQKIREELPNLESSEETEQINKHFHNTLEHLRLRRESPEADGPVYEGLVL 796
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Cavefish
Match: vps35 (VPS35 retromer complex component [Source:NCBI gene;Acc:103024195])

HSP 1 Score: 817.765 bits (2111), Expect = 0.000e+0
Identity = 423/789 (53.61%), Postives = 583/789 (73.89%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE 2424
            + QDEQE+LLDEA+  VK+QSFQMKR LDK+KLMD LKHA+NMLGELR   LSPKSYYELYMAI+DEL +LE YL +EF KGRKV+DLYELVQYAGNI+PRLYLLITVGVVY++    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD D EQ S E   G   DSIDF+LLNF+EMNKLWVRMQHQGH+R++EKRE+ER+ELR+LVGTNL RLSQLE ++V++YK  VL  VLEQ+VNC D++AQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+RL+ +A  E+  GIP  I LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++  ++    +N+  I+ SS +S+EL RLLK+P+D Y +I  +LQL++F  L +  +Y  RK +SCY+L+NTLD N  +  +EQ++++   +  L+ D P+    ++ DPE+F EEQ+LV + IHL    D   DQ Y IL   +K  GAGG +R   +  P+VF    LA RY       + W+KK +K+F   H+ +S +     AEL L+LFLQGAL    I  FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  T E+M C SEENH  LR+QCA A + +L+KK DQ RAV++  HLFWSG+  ++ G+E+RD K+++ CL K+ K+AN+C++ +++ QL IE+L+R++ F+   ++ ++   +N LI  IR+ LP++++  + + IN HF N + ++ ++++S + E
Sbjct:    7 SPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NILPD-DGEQ-SEEEMTGDINDSIDFVLLNFAEMNKLWVRMQHQGHSRDREKREKERQELRILVGTNLVRLSQLEGVNVEKYKQIVLAGVLEQVVNCRDSLAQEYLMECIIQVFPDEFHLQTLNLFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLESTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLQLKHFPPLFEYFDYESRKSMSCYVLSNTLDYNTTIVAQEQVDAILNLVSTLIQDQPD-QPAEDPDPEDFAEEQSLVGRFIHLLHSEDP--DQQYLILNTARKHFGAGGNQRIRYTLPPLVFAAYQLAFRYKDNSSSDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIG-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMRCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVSICAHLFWSGRNTDKNGEEIRDGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYVCFYERENDAVTVQVLNQLISKIREDLPNLEASEETEQINKHFHNTLEHLRLQRESPESE 787
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Sea Lamprey
Match: vps35 (VPS35 retromer complex component [Source:ZFIN;Acc:ZDB-GENE-030131-2042])

HSP 1 Score: 799.275 bits (2063), Expect = 0.000e+0
Identity = 418/790 (52.91%), Postives = 577/790 (73.04%), Query Frame = 1 
Query:   58 ATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIE-RLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDE 2424
            + Q+EQE+LLDEAL  VK+QSFQMKR LDK+KLMDGLKHA+NMLGELR   LSPK YYELYMA+TDEL HLE +L++EF KGRKV+DLYELVQYAGNI+PRLYLL+TVGVVYIK    SRKDIL+DLVEM RGVQHPLRGLFLRNYLLQC R ++LPD    + + +   G   DS+DF+LLNF+EMNKLWVRMQHQGH+REKEKRE+ER+ELR+LVGTNL RLSQLE ++V R+K  VLP +LEQ+VNC DAIAQEYLM+ +IQVFPDEFHL+TL   LRA +EL  NV++K+II ALI+ RL+ +A  E+  GIP  + LFDIFS++V ++++ R+D+P ED++SL+ SL++LA+K YP++++  DKV++   D    +N+  ++ SS +S+EL RLLK+P+D Y ++  ILQL++F  L +  +Y  RK +SCY+L N L+ +  +  ++Q++SL   +  L+ D P+    +E DPE+F EEQ LV + IHL +   ++ DQ Y IL   +K  GAGG +R   +  P+VF    LA  Y       + W+KK +K+F   H+ +  +     AEL L+L+LQGAL    I+ FE  E +AYEF SQAFSL+E+EI+DS+ Q+ AITLI  TLE+  C SEENH  LR+QCA A + +L+KK DQ RAV +  HLFWSG+  E  G ELRD K+++ CL K+ K+AN+C++ +++ QL  E+L+R+++F+   +E +S   +N LI+ IR+ LP+++S  + + ++ HF N + ++ ++Q+S + +
Sbjct:    3 SAQEEQEKLLDEALQVVKVQSFQMKRCLDKNKLMDGLKHASNMLGELRTSMLSPKCYYELYMAVTDELHHLEVFLIDEFTKGRKVADLYELVQYAGNIIPRLYLLVTVGVVYIKTFAQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTR-NVLPDEG--EVTDDKATGEIGDSLDFVLLNFAEMNKLWVRMQHQGHSREKEKRERERQELRILVGTNLVRLSQLEGVNVDRFKQVVLPGILEQVVNCRDAIAQEYLMECIIQVFPDEFHLQTLNLFLRACAELHQNVNVKNIIIALIDSRLALFAHREDGPGIPQEVKLFDIFSQQVATVIQSRQDMPTEDVVSLQVSLINLAMKCYPDRVDYVDKVLQTTADIFGKLNLEHVATSSAVSKELTRLLKIPVDSYNNVLTILQLKHFRPLFEYFDYQSRKNMSCYVLGNALEHDTNVPMQDQVDSLLTLVSSLVQDQPD-QPTEEPDPEDFAEEQGLVGRFIHLLK--AESPDQQYLILNTARKHFGAGGNQRIRHTLPPLVFAAYRLAFWYKECAQMDDKWEKKCQKIFTFCHQTIGALIKAELAELPLRLYLQGALAAGEIA-FENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLILGTLERCHCFSEENHEPLRTQCALAAS-KLLKKPDQCRAVTVCSHLFWSGRTTESGGNELRDGKRVMECLKKAVKIANQCMDTSLQVQLFTEILNRYIFFYEKGNEAVSIQVLNQLIQKIREELPNLESNEETEHLSKHFQNTLEHLRLRQESPESD 784
[Close]
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Yeast
Match: VPS35 (Endosomal subunit of membrane-associated retromer complex; required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p; overexpression of wild-type human VPS35 or Parkinson's-associated vps35-D686N or vps35-P299S variants complements Ni2+ resistance and Cd2+ sensitivity of yeast vps35 null mutant [Source:SGD;Acc:S000003690])

HSP 1 Score: 271.552 bits (693), Expect = 3.203e-78
Identity = 174/397 (43.83%), Postives = 244/397 (61.46%), Query Frame = 1 
Query:   55 MATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEE-------NSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSS 1224
            MA  D  E     A+  +K ++  M R L + KLM+ L+H + ML ELR   LSPK YYELY+ I D L +L +YL+E   +   ++DLYELVQY GN+VPRLYL+ITVG  Y+  NE  +K+IL+D++EM RGVQ+P+RGLFLR YL Q  + +LLP+ D    S             FI+ NF EMNKLWVR+QHQG  RE+E R +ER+EL++LVG+ L RLSQ+   + Q YK  +LP +LEQ++ C D ++QEYL+DV+ QVF DEFHL+TL+TLL+ T  L  +V I  I+  L++RL+ Y T +        ST     + +F  F   +  +   R DL  +  + L  S++ L+LK YPN  +  +K+ +  +   K+     IS  S
Sbjct:    1 MAYADSPEN----AIAVIKQRTALMNRCLSQHKLMESLQHTSIMLTELRNPNLSPKKYYELYVIIFDSLTNLSTYLIENHPQNHHLADLYELVQYTGNVVPRLYLMITVGTSYLTFNEAPKKEILKDMIEMCRGVQNPIRGLFLRYYLSQRTK-ELLPEDDPSFNS------------QFIMNNFIEMNKLWVRLQHQGPLRERETRTRERKELQILVGSQLVRLSQIIDDNFQMYKQDILPTILEQVIQCRDLVSQEYLLDVICQVFADEFHLKTLDTLLQTTLHLNPDVSINKIVLTLVDRLNDYVTRQLEDDPNATSTNAYLDMDVFGTFWDYLTVLNHERPDLSLQQFIPLVESVIVLSLKWYPNNFDNLNKLFELVLQKTKDYGQKNISLES 380

HSP 2 Score: 119.783 bits (299), Expect = 4.638e-28
Identity = 82/250 (32.80%), Postives = 143/250 (57.20%), Query Frame = 1 
Query: 1726 MEKVFKLFHECVSLI---ANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHF-ALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKE-------LRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEK---ISAARINSLIKSIRKLLPDIQSGNDGDAI---NIHFANVIGYIEVKQKS-ADD 2421
            ++++FK    C++ I    NN   +L LKL LQ A+  D++   E    ++Y+FFSQAF++FEE ++DS+ Q+ A+  I  +L+K   L +E ++ +L  +C   G+ +L+KK DQ RAV L  HL+W+ + +   G+E        RD K+++ CL +S ++A+  ++     +L++E+L+R LY+F    E    IS   IN LI+ I+  L  ++  ++  ++   +I   ++ G   VK  S ADD
Sbjct:  623 LKQMFKFVSRCINDIFNSCNNSCTDLILKLNLQCAILADQLQLNE----ISYDFFSQAFTIFEESLSDSKTQLQALIYIAQSLQKTRSLYKEAYYDSLIVRCTLHGS-KLLKKQDQCRAVYLCSHLWWATE-ISNIGEEEGITDNFYRDGKRVLECLQRSLRVADSIMDNEQSCELMVEILNRCLYYFIHGDESETHISIKYINGLIELIKTNLKSLKLEDNSASMITNSISDLHITGENNVKASSNADD 866
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Nematostella
Match: EDO45995 (Vacuolar protein sorting-associated protein 35 [Source:UniProtKB/TrEMBL;Acc:A7RRD4])

HSP 1 Score: 733.021 bits (1891), Expect = 0.000e+0
Identity = 391/770 (50.78%), Postives = 549/770 (71.30%), Query Frame = 1 
Query:  127 MKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPD-TDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDEVFS 2433
            MKR LDK KLMDGLKHA+NML ELR   LSPKSYYELYMAI+DELRHLE +L++EFQKGRKVSDLYELVQYAGNIVPRLYLLITVG+VYIK  E  RKDIL+DLVEM RGVQHPLRGLFLRNYLLQ  R ++LPD  D  +  PE + G+ +DSIDF+LLNFSEMNKLWVR+QHQGH+RE++KRE+ER+ELR+LVGTNL RLSQLE +    YK  VLP +LEQ +NC D IAQEYLM+ +IQVFPDE+HL+T+   L + +EL   V+IK+II +LI+RL+ +A  ++  GIP  I LFD+ S++V+ +++ R D+  ED +SL+ SL++LALK YP++++  DKV++   +    + I  I  S+ +S+EL RLLK PID Y ++  +L+L+ F  +    ++  RK +S Y+++N ++   V+ T+EQ+++L   +  L+ D  E    +  DPE+F EEQ+++ K + L +   DN DQ Y IL   +K  G+GG KR   +  PIVF    LA +Y    +E + W KK +K+F+  H+ ++ +A    AEL+L+LFLQGA+   ++  F   E +AYEF SQAFS++E+EI+DS+ Q+ AITLI  T E+M C  EENH  LR+QCA A + +L+KK DQ RAVA+  HLFWSGK  +  G E  D K+++ CL K+ ++AN+C++ T++ QL +E+L+ +LY++  N++ ++A  +N L+  IR+ LP ++S  + + I+ HF N I ++E K++S +++  S
Sbjct:    1 MKRCLDKGKLMDGLKHASNMLSELRTSLLSPKSYYELYMAISDELRHLELHLVDEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGIVYIKAKEAPRKDILKDLVEMCRGVQHPLRGLFLRNYLLQSTR-NMLPDINDDNEDRPEHD-GSVKDSIDFVLLNFSEMNKLWVRIQHQGHSRERQKRERERQELRILVGTNLVRLSQLEGVTSDVYKKLVLPGILEQTINCKDPIAQEYLMECIIQVFPDEYHLQTITQFLSSCTELHPAVNIKNIIISLIDRLALFANRDDG-GIPTDIKLFDLMSEQVSKVIQMRTDMATEDKVSLQVSLVNLALKCYPDRVDYVDKVLEYTSELFSKLEIESIDKSNPISKELTRLLKNPIDSYNNVLTLLELKFFIPMFNYFDFTTRKEMSLYVVSNAVESEVVIPTQEQVDTLLTLVSTLVADQ-EDQPSEPTDPEDFAEEQHMMGKFLTLMK--SDNADQQYLILNTARKHFGSGGEKRIKFTLPPIVFSAYQLAFQYGNAKEEDDKWDKKCQKIFQFCHQTITALAKAEYAELSLRLFLQGAMAAGKVG-FSTSETVAYEFMSQAFSIYEDEISDSKSQLAAITLIICTFEQMSCFGEENHEPLRTQCALAAS-KLLKKPDQCRAVAVCSHLFWSGKSKDIEGGECHDGKRVMECLKKAVRIANQCMDATVQVQLFVEILNCYLYYYERNTDTVTATILNQLLDKIREDLPGLESNEETEQISKHFKNTISHMEAKKQSKEEDAPS 762
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Nematostella
Match: EDO28847 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5B7])

HSP 1 Score: 185.652 bits (470), Expect = 4.340e-54
Identity = 87/188 (46.28%), Postives = 132/188 (70.21%), Query Frame = 1 
Query: 1714 WQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKI 2277
            W  K +K+F+  H+ ++ +A    AEL+L+LFLQGA+   ++  F   E +AYEF SQAFS++E+EI+DS+ Q+ AITLI  T E+M C  EENH  LR+QCA A A +L+KK DQ RAVA+  HLFWSGK  +  G E  D K+++ CL K+ ++AN+C++ T++ QL +E+L+ +LY++  N++ +
Sbjct:    8 WDNKCQKIFQFCHQTITALAKAEYAELSLRLFLQGAMAAGKVG-FSTSETVAYEFMSQAFSIYEDEISDSKSQLAAITLIICTFEQMSCFGEENHEPLRTQCALA-ASKLLKKPDQCRAVAVCSHLFWSGKSKDIEGGECHDGKRVMECLKKAVRIANQCMDATVQVQLFVEILNCYLYYYERNTDTV 193
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Planmine SMEST
Match: SMESG000064165.1 (SMESG000064165.1)

HSP 1 Score: 1559.27 bits (4036), Expect = 0.000e+0
Identity = 797/797 (100.00%), Postives = 797/797 (100.00%), Query Frame = 1 
Query:   55 MATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTXXXXXXXXXXXXXXXXVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSXXXXXXXXXXXXQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDEVFSQLII 2445
            MATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTREKEKREQERRELRLLVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSEDEVDPEEFEEEQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDEVFSQLII
Sbjct:    1 MATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRAHQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIVPRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQCLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGHTREKEKREQERRELRLLVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVNCHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGALIERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEASLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLLKMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLNTEEQLESLFKCIQVLLYDSPEINSEDEVDPEEFEEEQNLVAKSIHLFRFSDDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDETEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEKRELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFALRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLISCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLIKSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDEVFSQLII 797
The following BLAST results are available for this feature:
BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
VPS350.000e+052.88VPS35 retromer complex component [Source:HGNC Symb... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
vps-350.000e+043.04Vacuolar protein sorting-associated protein 35 [S... [more]
vps-350.000e+043.04Vacuolar protein sorting-associated protein 35 [S... [more]
vps-350.000e+043.07Vacuolar protein sorting-associated protein 35 [S... [more]
vps-350.000e+043.07Vacuolar protein sorting-associated protein 35 [S... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Vps350.000e+047.05gene:FBgn0034708 transcript:FBtr0071771[more]
Vps350.000e+047.05gene:FBgn0034708 transcript:FBtr0071772[more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
vps350.000e+052.85VPS35 retromer complex component [Source:ZFIN;Acc:... [more]
vps356.630e-7976.05VPS35 retromer complex component [Source:ZFIN;Acc:... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
vps350.000e+052.51VPS35 retromer complex component [Source:NCBI gene... [more]
vps350.000e+051.50VPS35 retromer complex component [Source:NCBI gene... [more]
vps350.000e+050.78VPS35 retromer complex component [Source:NCBI gene... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Vps350.000e+052.88VPS35 retromer complex component [Source:MGI Symbo... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q2HJG5|VPS35_BOVIN0.000e+053.00Vacuolar protein sorting-associated protein 35 OS=... [more]
sp|Q9EQH3|VPS35_MOUSE0.000e+052.88Vacuolar protein sorting-associated protein 35 OS=... [more]
sp|Q96QK1|VPS35_HUMAN0.000e+052.88Vacuolar protein sorting-associated protein 35 OS=... [more]
sp|Q54C24|VPS35_DICDI4.956e-16739.09Vacuolar protein sorting-associated protein 35 OS=... [more]
sp|Q7X659|VP35A_ARATH6.139e-16340.87Vacuolar protein sorting-associated protein 35A OS... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A226NJQ20.000e+053.74Vacuolar protein sorting-associated protein 35 OS=... [more]
A0A091P0820.000e+053.38Vacuolar protein sorting-associated protein 35 (Fr... [more]
K9IZN20.000e+053.60Vacuolar protein sorting-associated protein 35 OS=... [more]
A0A3B4DYB90.000e+053.61Vacuolar protein sorting-associated protein 35 OS=... [more]
A0A3B3RM190.000e+053.44Vacuolar protein sorting-associated protein 35 OS=... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
vps350.000e+053.61VPS35 retromer complex component [Source:NCBI gene... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
vps350.000e+052.91VPS35 retromer complex component [Source:ZFIN;Acc:... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
VPS353.203e-7843.83Endosomal subunit of membrane-associated retromer ... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO459950.000e+050.78Vacuolar protein sorting-associated protein 35 [S... [more]
EDO288474.340e-5446.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vacuolar protein sorting-associated protein 35 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064165.10.000e+0100.00SMESG000064165.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030726 ID=SMED30030726|Name=Vacuolar protein sorting-associated protein 35|organism=Schmidtea mediterranea sexual|type=transcript|length=2620bp
GCGCTTTATCAAATTTATTTATTATTATATTCGGAAGCCAAATTTTGATA
TTTAATGGCAACGCAAGATGAACAAGAAAGGCTATTAGATGAAGCGTTAA
CTGAAGTTAAGCTGCAATCTTTTCAAATGAAACGATCACTAGATAAAAGT
AAACTTATGGATGGTTTAAAACACGCTACAAACATGCTTGGTGAATTGCG
AGCTCATCAGTTGTCTCCAAAATCTTACTATGAACTATATATGGCTATAA
CTGATGAATTGCGTCATCTTGAAAGTTATTTATTGGAAGAATTTCAAAAA
GGTCGAAAAGTAAGCGACCTTTATGAATTAGTGCAATATGCTGGAAATAT
AGTTCCAAGATTGTATTTGCTTATCACTGTTGGAGTTGTTTATATTAAAT
GCAATGAATTATCGAGGAAGGATATTTTACGTGATCTTGTTGAGATGACT
AGAGGGGTTCAACATCCTTTGAGAGGATTATTCTTGCGAAATTATTTACT
TCAGTGTCTTCGTGTAGACCTTTTGCCCGATACAGATATCGAACAAGGCT
CACCGGAATCTGAGCAGGGAACAACGCAAGATAGTATTGATTTTATATTA
TTGAATTTTTCTGAAATGAATAAATTGTGGGTCCGTATGCAACATCAAGG
CCATACGAGGGAAAAGGAAAAACGCGAACAAGAAAGAAGAGAATTGAGAT
TGTTAGTTGGAACGAATTTAAATCGGCTCAGTCAATTGGAATCGATTGAC
GTACAAAGATACAAAACTACTGTTCTTCCTGCAGTTTTGGAACAAATCGT
TAATTGCCACGATGCAATCGCTCAAGAATATCTAATGGATGTCGTTATTC
AGGTTTTCCCTGACGAATTCCATTTAGAAACCCTGGAAACTCTTTTAAGA
GCTACTTCGGAATTGGAGTCAAATGTACATATTAAATCTATTATTGGGGC
ATTGATTGAACGTCTCTCTCAATATGCTACAGAAGAAAATAGCACTGGAA
TTCCGGATAGCATTCCTTTATTTGATATATTTTCAAAAGAAGTTAATTCA
ATGTTAGAACGTCGCAAAGACTTACCTCCGGAAGACATAATGTCTCTCGA
AGCGTCTTTACTACATTTGGCTCTGAAAGTTTATCCAAACAAATTGGAAT
TAGATGACAAGGTCATCAAAGGGGCCATAGATGCATTGAAAAATATGAAT
ATATCGAAAATTAGTTATAGTTCTTGTTTATCAAGAGAATTAATTCGTTT
GCTAAAAATGCCAATAGATGTGTATAAAGATATTCGAATAATTCTTCAAT
TAGAAAATTTTTCAGAATTGATTCAAAATTTAGAATATAATGGACGAAAA
CTAATATCGTGTTATTTGTTAACAAATACACTTGATCAGAATTATGTTTT
GAACACAGAAGAACAATTGGAAAGTCTATTTAAATGTATCCAAGTATTGT
TATATGACAGTCCAGAAATAAATTCCGAAGACGAAGTTGATCCTGAAGAA
TTCGAAGAAGAGCAAAATCTTGTTGCAAAATCCATTCATTTGTTTCGATT
TAGTGATGATAATCTTGATCAAAACTATAAAATTCTTATTAAAATGAAAA
AGATGCTTGGTGCTGGAGGTGTCAAGCGATTTTTACGCTCATTTATACCA
ATAGTATTTCAAACATTGAATTTAGCTACTCGCTATCACACTCGATATGA
TGAGACAGAAATTTGGCAGAAAAAAATGGAAAAAGTTTTCAAACTGTTTC
ATGAATGCGTGTCGCTTATTGCAAACAATGGTGCTGCTGAATTAGCTCTA
AAATTATTTTTGCAAGGCGCATTAACTATTGATAGAATAAGTAAATTTGA
AAAACGAGAACTTTTGGCATATGAATTTTTTTCTCAGGCCTTTTCATTAT
TCGAAGAAGAAATAGCGGATTCTAGAATACAGATCAACGCAATTACATTA
ATCACGTCTACTTTAGAAAAAATGTGCTGTTTGAGTGAAGAAAATCATTT
CGCATTGAGATCCCAATGTGCCAAAGCTGGTGCTCTTCGTTTGGTGAAGA
AAATGGACCAAAGTCGTGCAGTTGCATTGGCTGGTCATTTATTTTGGTCA
GGGAAACCATTAGAAGAGTACGGAAAAGAGTTACGTGACCATAAGAAACT
TATTTCTTGTCTTGATAAATCGGAAAAGCTGGCCAATGAATGTCTTGAAG
GTACGATGAAAGCCCAACTTTTAATAGAGCTTCTTAGTCGATTTCTATAT
TTCTTCGCCATAAATTCAGAAAAGATTTCAGCAGCGAGAATTAATTCCTT
GATTAAAAGTATTCGAAAGTTATTGCCAGATATTCAAAGTGGAAATGATG
GGGATGCCATAAATATCCATTTTGCGAATGTTATTGGCTACATAGAAGTG
AAACAGAAATCCGCTGATGATGAAGTATTTTCCCAATTAATTATTTAGTT
TGAAAATAAAGAAATGGACAAATTTTTCTGTGATTTTAGTCAAAAATTTT
AACAGTTAAAATTAAGTTGTGCATTGTTTGATTTCCATTGGACTCTGTTT
TGCAAGATTTATGGCATCTTTTGTAGTAGTGTGAGTGATCATTAAATATA
CAATAAATCTGAATAATTTC
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protein sequence of SMED30030726-orf-1

>SMED30030726-orf-1 ID=SMED30030726-orf-1|Name=SMED30030726-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=798bp
MATQDEQERLLDEALTEVKLQSFQMKRSLDKSKLMDGLKHATNMLGELRA
HQLSPKSYYELYMAITDELRHLESYLLEEFQKGRKVSDLYELVQYAGNIV
PRLYLLITVGVVYIKCNELSRKDILRDLVEMTRGVQHPLRGLFLRNYLLQ
CLRVDLLPDTDIEQGSPESEQGTTQDSIDFILLNFSEMNKLWVRMQHQGH
TREKEKREQERRELRLLVGTNLNRLSQLESIDVQRYKTTVLPAVLEQIVN
CHDAIAQEYLMDVVIQVFPDEFHLETLETLLRATSELESNVHIKSIIGAL
IERLSQYATEENSTGIPDSIPLFDIFSKEVNSMLERRKDLPPEDIMSLEA
SLLHLALKVYPNKLELDDKVIKGAIDALKNMNISKISYSSCLSRELIRLL
KMPIDVYKDIRIILQLENFSELIQNLEYNGRKLISCYLLTNTLDQNYVLN
TEEQLESLFKCIQVLLYDSPEINSEDEVDPEEFEEEQNLVAKSIHLFRFS
DDNLDQNYKILIKMKKMLGAGGVKRFLRSFIPIVFQTLNLATRYHTRYDE
TEIWQKKMEKVFKLFHECVSLIANNGAAELALKLFLQGALTIDRISKFEK
RELLAYEFFSQAFSLFEEEIADSRIQINAITLITSTLEKMCCLSEENHFA
LRSQCAKAGALRLVKKMDQSRAVALAGHLFWSGKPLEEYGKELRDHKKLI
SCLDKSEKLANECLEGTMKAQLLIELLSRFLYFFAINSEKISAARINSLI
KSIRKLLPDIQSGNDGDAINIHFANVIGYIEVKQKSADDEVFSQLII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR042491Vps35_C
IPR005378Vps35
Vocabulary: molecular function
TermDefinition
GO:0008565protein transporter activity
GO:0031748D1 dopamine receptor binding
Vocabulary: biological process
TermDefinition
GO:0042147retrograde transport, endosome to Golgi
GO:0015031protein transport
GO:0006810transport
GO:0007040lysosome organization
GO:0010628positive regulation of gene expression
GO:0010629negative regulation of gene expression
GO:0010821regulation of mitochondrion organization
GO:0031647regulation of protein stability
GO:0031648protein destabilization
GO:0032268regulation of cellular protein metabolic process
GO:0032463negative regulation of protein homooligomerization
GO:0033365protein localization to organelle
GO:0043653mitochondrial fragmentation involved in apoptotic process
GO:0045056transcytosis
GO:0050882voluntary musculoskeletal movement
GO:0060161positive regulation of dopamine receptor signaling pathway
GO:0061357positive regulation of Wnt protein secretion
GO:0090141positive regulation of mitochondrial fission
GO:0090263positive regulation of canonical Wnt signaling pathway
GO:0090326positive regulation of locomotion involved in locomotory behavior
GO:0099074mitochondrion to lysosome transport
GO:0099639neurotransmitter receptor transport, endosome to plasma membrane
GO:1901215negative regulation of neuron death
GO:1902823negative regulation of late endosome to lysosome transport
GO:1902950regulation of dendritic spine maintenance
GO:1903181positive regulation of dopamine biosynthetic process
GO:1903364positive regulation of cellular protein catabolic process
GO:1903828negative regulation of cellular protein localization
GO:1905166negative regulation of lysosomal protein catabolic process
GO:1990126retrograde transport, endosome to plasma membrane
GO:2000331regulation of terminal button organization
Vocabulary: cellular component
TermDefinition
GO:0030904retromer complex
GO:0005737cytoplasm
GO:0005739mitochondrion
GO:0005764lysosome
GO:0005765lysosomal membrane
GO:0005768endosome
GO:0005769early endosome
GO:0005770late endosome
GO:0005829cytosol
GO:0010008endosome membrane
GO:0014069postsynaptic density
GO:0016020membrane
GO:0030906retromer, cargo-selective complex
GO:0043005neuron projection
GO:0043025neuronal cell body
GO:0048471perinuclear region of cytoplasm
GO:0070062extracellular exosome
GO:0097422tubular endosome
GO:0099073mitochondrion-derived vesicle
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005378Vacuolar protein sorting-associated protein 35PFAMPF03635Vps35coord: 10..755
e-value: 7.5E-256
score: 851.2
IPR005378Vacuolar protein sorting-associated protein 35PIRSFPIRSF009375Retromer_Vps35coord: 1..321
e-value: 9.0E-154
score: 511.5
coord: 309..796
e-value: 4.0E-94
score: 314.1
IPR005378Vacuolar protein sorting-associated protein 35PANTHERPTHR11099VACUOLAR SORTING PROTEIN 35coord: 3..786
IPR042491Vacuolar protein sorting-associated protein 35, C-terminalGENE3DG3DSA:1.25.40.660coord: 482..783
e-value: 6.1E-85
score: 287.1
NoneNo IPR availablePANTHERPTHR11099:SF0VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35coord: 3..786