Midasin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030711 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 346.665 bits (888), Expect = 3.772e-107 Identity = 178/347 (51.30%), Postives = 241/347 (69.45%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQL----VNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+ R+VSAG PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y +S+++ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+ + ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y ++ +DW + + +GY+LL G+ R ++E++++ +L + F+ +L + FS N+ K++ + +Q+ N +I WT RR+A+L R+++ EPVLLVG+
Sbjct: 1056 YILTSSVKLNLRDIVRVVSAG-TYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCY----TSDSSGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGFITLRDLFRWAER-YRLAEPTEKEYDWLQHLANDGYMLLAGRVRKQEEIDVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKLSTQISTLECNFGHIVWTEGMRRLAMLVGRALEFGEPVLLVGD 1391 HSP 2 Score: 115.931 bits (289), Expect = 1.537e-27 Identity = 76/237 (32.07%), Postives = 123/237 (51.90%), Query Frame = 1
Query: 64 SVSKNLTDLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
S + R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +++G L+ A++ GHW++LDELNLA +LE LN D EI++ E + + H ++F QNP GGRK L R+ NRF ++ +P+ ++ E + + I KN +KMV ++ E V ++ + +LRDLFRW +
Sbjct: 1728 SAGTTAMNAQRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKG-GEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDM-EFIASTLFPAIEKNIVKKMVAFNNQIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQ 1956 HSP 3 Score: 83.5741 bits (205), Expect = 1.066e-16 Identity = 63/203 (31.03%), Postives = 103/203 (50.74%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG--RKVLSRA--LRNRFIELHFNPIPNCELVEIL 594
R + E + ++ Y + ESV K+L LA V++ VL++G GKTSL+ YLA +TG +V + TD + +G Y ++ V++ G L QA GHWI+L++++ AP D++ L +L +N E+ I +K P F+ FAT+ G R + S A L + ++H + + EL E+L
Sbjct: 289 RSSSREQELALRSYVLVESVCKSLQTLAMAVASQNA--VLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLL-ENGELLIPGRGDCLKVAPGFQFFATRRLLSCGGNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVL 485 HSP 4 Score: 82.0333 bits (201), Expect = 3.123e-16 Identity = 57/181 (31.49%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSSE-----TTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
TE + + + R + G+ PVL+ G+T GKT++ A L + V+ H + +++G V+Q ++ T+ + +G LVQAM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1366 TEGMRRLAMLVGRALEFGE--PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGDF-GKKELSPALRNRFTEI 1543 HSP 5 Score: 77.411 bits (189), Expect = 1.115e-14 Identity = 61/183 (33.33%), Postives = 96/183 (52.46%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T +TL+F EG L QA++ G WI+LDE+NLA +ILE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E +++L + + KN + ++ L+ ES +L R + SLR L R R + N C
Sbjct: 801 NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 979 HSP 6 Score: 56.9954 bits (136), Expect = 4.600e-8 Identity = 33/108 (30.56%), Postives = 60/108 (55.56%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
Q S + T + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + TI +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2211 TQLASGHSHGTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDP--VHGD---ISRAMRNRGLEIYIS 2313 HSP 7 Score: 51.6026 bits (122), Expect = 2.288e-6 Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S + LA VS G+ PVL+ GET GKTS I YLA +TGH VN ++ +D + +G Y
Sbjct: 650 FAATRPSSVLIEQLAVCVSKGE--PVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGY 716
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 346.665 bits (888), Expect = 3.772e-107 Identity = 178/347 (51.30%), Postives = 241/347 (69.45%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQL----VNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+ R+VSAG PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y +S+++ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+ + ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y ++ +DW + + +GY+LL G+ R ++E++++ +L + F+ +L + FS N+ K++ + +Q+ N +I WT RR+A+L R+++ EPVLLVG+
Sbjct: 1056 YILTSSVKLNLRDIVRVVSAG-TYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCY----TSDSSGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGFITLRDLFRWAER-YRLAEPTEKEYDWLQHLANDGYMLLAGRVRKQEEIDVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKLSTQISTLECNFGHIVWTEGMRRLAMLVGRALEFGEPVLLVGD 1391 HSP 2 Score: 115.931 bits (289), Expect = 1.537e-27 Identity = 76/237 (32.07%), Postives = 123/237 (51.90%), Query Frame = 1
Query: 64 SVSKNLTDLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
S + R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +++G L+ A++ GHW++LDELNLA +LE LN D EI++ E + + H ++F QNP GGRK L R+ NRF ++ +P+ ++ E + + I KN +KMV ++ E V ++ + +LRDLFRW +
Sbjct: 1728 SAGTTAMNAQRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKG-GEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDM-EFIASTLFPAIEKNIVKKMVAFNNQIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQ 1956 HSP 3 Score: 83.5741 bits (205), Expect = 1.066e-16 Identity = 63/203 (31.03%), Postives = 103/203 (50.74%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG--RKVLSRA--LRNRFIELHFNPIPNCELVEIL 594
R + E + ++ Y + ESV K+L LA V++ VL++G GKTSL+ YLA +TG +V + TD + +G Y ++ V++ G L QA GHWI+L++++ AP D++ L +L +N E+ I +K P F+ FAT+ G R + S A L + ++H + + EL E+L
Sbjct: 289 RSSSREQELALRSYVLVESVCKSLQTLAMAVASQNA--VLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLL-ENGELLIPGRGDCLKVAPGFQFFATRRLLSCGGNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVL 485 HSP 4 Score: 82.0333 bits (201), Expect = 3.123e-16 Identity = 57/181 (31.49%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSSE-----TTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
TE + + + R + G+ PVL+ G+T GKT++ A L + V+ H + +++G V+Q ++ T+ + +G LVQAM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1366 TEGMRRLAMLVGRALEFGE--PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGDF-GKKELSPALRNRFTEI 1543 HSP 5 Score: 77.411 bits (189), Expect = 1.115e-14 Identity = 61/183 (33.33%), Postives = 96/183 (52.46%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T +TL+F EG L QA++ G WI+LDE+NLA +ILE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E +++L + + KN + ++ L+ ES +L R + SLR L R R + N C
Sbjct: 801 NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 979 HSP 6 Score: 56.9954 bits (136), Expect = 4.600e-8 Identity = 33/108 (30.56%), Postives = 60/108 (55.56%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
Q S + T + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + TI +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2211 TQLASGHSHGTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDP--VHGD---ISRAMRNRGLEIYIS 2313 HSP 7 Score: 51.6026 bits (122), Expect = 2.288e-6 Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S + LA VS G+ PVL+ GET GKTS I YLA +TGH VN ++ +D + +G Y
Sbjct: 650 FAATRPSSVLIEQLAVCVSKGE--PVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGY 716
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 346.665 bits (888), Expect = 3.772e-107 Identity = 178/347 (51.30%), Postives = 241/347 (69.45%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQL----VNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+ R+VSAG PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y +S+++ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+ + ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y ++ +DW + + +GY+LL G+ R ++E++++ +L + F+ +L + FS N+ K++ + +Q+ N +I WT RR+A+L R+++ EPVLLVG+
Sbjct: 1056 YILTSSVKLNLRDIVRVVSAG-TYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCY----TSDSSGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGFITLRDLFRWAER-YRLAEPTEKEYDWLQHLANDGYMLLAGRVRKQEEIDVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKLSTQISTLECNFGHIVWTEGMRRLAMLVGRALEFGEPVLLVGD 1391 HSP 2 Score: 115.931 bits (289), Expect = 1.537e-27 Identity = 76/237 (32.07%), Postives = 123/237 (51.90%), Query Frame = 1
Query: 64 SVSKNLTDLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
S + R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +++G L+ A++ GHW++LDELNLA +LE LN D EI++ E + + H ++F QNP GGRK L R+ NRF ++ +P+ ++ E + + I KN +KMV ++ E V ++ + +LRDLFRW +
Sbjct: 1728 SAGTTAMNAQRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKG-GEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDM-EFIASTLFPAIEKNIVKKMVAFNNQIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQ 1956 HSP 3 Score: 83.5741 bits (205), Expect = 1.066e-16 Identity = 63/203 (31.03%), Postives = 103/203 (50.74%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG--RKVLSRA--LRNRFIELHFNPIPNCELVEIL 594
R + E + ++ Y + ESV K+L LA V++ VL++G GKTSL+ YLA +TG +V + TD + +G Y ++ V++ G L QA GHWI+L++++ AP D++ L +L +N E+ I +K P F+ FAT+ G R + S A L + ++H + + EL E+L
Sbjct: 289 RSSSREQELALRSYVLVESVCKSLQTLAMAVASQNA--VLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLL-ENGELLIPGRGDCLKVAPGFQFFATRRLLSCGGNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVL 485 HSP 4 Score: 82.0333 bits (201), Expect = 3.123e-16 Identity = 57/181 (31.49%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSSE-----TTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
TE + + + R + G+ PVL+ G+T GKT++ A L + V+ H + +++G V+Q ++ T+ + +G LVQAM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1366 TEGMRRLAMLVGRALEFGE--PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGDF-GKKELSPALRNRFTEI 1543 HSP 5 Score: 77.411 bits (189), Expect = 1.115e-14 Identity = 61/183 (33.33%), Postives = 96/183 (52.46%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T +TL+F EG L QA++ G WI+LDE+NLA +ILE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E +++L + + KN + ++ L+ ES +L R + SLR L R R + N C
Sbjct: 801 NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 979 HSP 6 Score: 56.9954 bits (136), Expect = 4.600e-8 Identity = 33/108 (30.56%), Postives = 60/108 (55.56%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
Q S + T + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + TI +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2211 TQLASGHSHGTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDP--VHGD---ISRAMRNRGLEIYIS 2313 HSP 7 Score: 51.6026 bits (122), Expect = 2.288e-6 Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S + LA VS G+ PVL+ GET GKTS I YLA +TGH VN ++ +D + +G Y
Sbjct: 650 FAATRPSSVLIEQLAVCVSKGE--PVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGY 716
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 346.665 bits (888), Expect = 3.772e-107 Identity = 178/347 (51.30%), Postives = 241/347 (69.45%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQL----VNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+ R+VSAG PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y +S+++ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+ + ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y ++ +DW + + +GY+LL G+ R ++E++++ +L + F+ +L + FS N+ K++ + +Q+ N +I WT RR+A+L R+++ EPVLLVG+
Sbjct: 1056 YILTSSVKLNLRDIVRVVSAG-TYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCY----TSDSSGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGFITLRDLFRWAER-YRLAEPTEKEYDWLQHLANDGYMLLAGRVRKQEEIDVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKLSTQISTLECNFGHIVWTEGMRRLAMLVGRALEFGEPVLLVGD 1391 HSP 2 Score: 115.931 bits (289), Expect = 1.537e-27 Identity = 76/237 (32.07%), Postives = 123/237 (51.90%), Query Frame = 1
Query: 64 SVSKNLTDLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
S + R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +++G L+ A++ GHW++LDELNLA +LE LN D EI++ E + + H ++F QNP GGRK L R+ NRF ++ +P+ ++ E + + I KN +KMV ++ E V ++ + +LRDLFRW +
Sbjct: 1728 SAGTTAMNAQRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKG-GEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDM-EFIASTLFPAIEKNIVKKMVAFNNQIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQ 1956 HSP 3 Score: 83.5741 bits (205), Expect = 1.066e-16 Identity = 63/203 (31.03%), Postives = 103/203 (50.74%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG--RKVLSRA--LRNRFIELHFNPIPNCELVEIL 594
R + E + ++ Y + ESV K+L LA V++ VL++G GKTSL+ YLA +TG +V + TD + +G Y ++ V++ G L QA GHWI+L++++ AP D++ L +L +N E+ I +K P F+ FAT+ G R + S A L + ++H + + EL E+L
Sbjct: 289 RSSSREQELALRSYVLVESVCKSLQTLAMAVASQNA--VLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLL-ENGELLIPGRGDCLKVAPGFQFFATRRLLSCGGNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVL 485 HSP 4 Score: 82.0333 bits (201), Expect = 3.123e-16 Identity = 57/181 (31.49%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSSE-----TTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
TE + + + R + G+ PVL+ G+T GKT++ A L + V+ H + +++G V+Q ++ T+ + +G LVQAM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1366 TEGMRRLAMLVGRALEFGE--PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGDF-GKKELSPALRNRFTEI 1543 HSP 5 Score: 77.411 bits (189), Expect = 1.115e-14 Identity = 61/183 (33.33%), Postives = 96/183 (52.46%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T +TL+F EG L QA++ G WI+LDE+NLA +ILE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E +++L + + KN + ++ L+ ES +L R + SLR L R R + N C
Sbjct: 801 NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 979 HSP 6 Score: 56.9954 bits (136), Expect = 4.600e-8 Identity = 33/108 (30.56%), Postives = 60/108 (55.56%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
Q S + T + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + TI +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2211 TQLASGHSHGTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDP--VHGD---ISRAMRNRGLEIYIS 2313 HSP 7 Score: 51.6026 bits (122), Expect = 2.288e-6 Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S + LA VS G+ PVL+ GET GKTS I YLA +TGH VN ++ +D + +G Y
Sbjct: 650 FAATRPSSVLIEQLAVCVSKGE--PVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGY 716
BLAST of Midasin vs. Ensembl Human
Match: MDN1 (midasin AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:18302]) HSP 1 Score: 270.781 bits (691), Expect = 1.830e-81 Identity = 132/204 (64.71%), Postives = 159/204 (77.94%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
Y +T SV NL D+ R+VSAG PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y +S+++ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+ + ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ
Sbjct: 983 YILTSSVKLNLRDIVRVVSAG-TYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCY----TSDSSGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQ 1181 HSP 2 Score: 77.7962 bits (190), Expect = 6.464e-15 Identity = 61/183 (33.33%), Postives = 96/183 (52.46%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T +TL+F EG L QA++ G WI+LDE+NLA +ILE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E +++L + + KN + ++ L+ ES +L R + SLR L R R + N C
Sbjct: 728 NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 906 HSP 3 Score: 70.0922 bits (170), Expect = 1.938e-12 Identity = 47/148 (31.76%), Postives = 78/148 (52.70%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDIL-----EALNRVLDDNREIFI 426
R + E + ++ Y + ESV K+L LA V++ VL++G GKTSL+ YLA +TG +V + TD + +G Y ++ V++ G L QA GHWI+L++++ AP D++ +L V+D +I+I
Sbjct: 289 RSSSREQELALRSYVLVESVCKSLQTLAMAVASQNA--VLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVLQSRYPSLLAVVDHLLDIYI 431 HSP 4 Score: 51.2174 bits (121), Expect = 2.118e-6 Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S + LA VS G+ PVL+ GET GKTS I YLA +TGH VN ++ +D + +G Y
Sbjct: 577 FAATRPSSVLIEQLAVCVSKGE--PVLLVGETGTGKTSTIQYLAHITGHRLRVVNMNQQSDTADLLGGY 643
BLAST of Midasin vs. Ensembl Celegans
Match: F55F10.1 (Midasin [Source:UniProtKB/TrEMBL;Acc:O61851]) HSP 1 Score: 278.87 bits (712), Expect = 4.229e-84 Identity = 153/344 (44.48%), Postives = 221/344 (64.24%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARS-KDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITK-IILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y IT++V NL+++ARIV +G+ P+L++GETS GKTS++ +L+K TG+ R+NNHE TD+QEY+GSYV ++ LVF+EG LVQA+R G W+ILDELNLAPTD++EALNR+LDDNRE+F+ E TIKAHP FRLFATQNP G Y GRK LSRAL +RFI L F+ +P EL E++ C +P N KM+E + +L+ + + ++LRD+FRWA+R+ + DW + ++ GY LL G+ R+ KD+V +V + + R +++ E FS + + + +I EK + T RR+ VL ++ +E VL+VG
Sbjct: 724 YVITKTVKGNLSEIARIVCSGR-FPILLEGETSAGKTSIVCHLSKATGNRIVRINNHEHTDVQEYMGSYV----ADAGGRLVFREGALVQAVRDGSWVILDELNLAPTDVIEALNRLLDDNRELFVPEINETIKAHPRFRLFATQNPAGSYAGRKRLSRALMSRFIVLRFHHLPIEELSEMVCARCGVPPNASLKMIEVLNELRNRRSLSGLFSARDGLMTLRDVFRWAKRMTTDETTD----DWLQILVNHGYFLLAGRCRNEKDDVTVVETLEKVIKR----KIDKEALFSLDSPYMPRDVITEK---------VVLTLGMRRMLVLTEQAWLRNEAVLMVG 1045 HSP 2 Score: 108.612 bits (270), Expect = 1.963e-25 Identity = 69/210 (32.86%), Postives = 109/210 (51.90%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE--TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEIL---DESCKIPKNKGRKMVEAMRKL-QIESQELDVVHCKRNYISLRDLFRW 732
P+L++G GK+S + LA+LTG+ R+N + TD+ + GS V + + T T +++G +++A++ G WI+LDE+NLA +LE LN D R ++I E + I A R FA QNP G R+ L ++ NRF ++ N + ++ +L DE + + MV +L QI H +LRDL RW
Sbjct: 1376 PILLEGAPGCGKSSTVMALAQLTGNPITRLNLSDQTDLSDLFGSDVPVLTEDGTMTFRWEDGPVLKAIKKGEWILLDEMNLASQSVLEGLNACFDHRRVLYIAELNREFEIPATSNCRFFACQNPRVQGGNRRALPKSFINRFTNIYTNDLTKNDIEIVLNGVDEKSLLRTEQRSAMVAISEQLEQISGSFSGGPHS----FNLRDLLRW 1581 HSP 3 Score: 85.1149 bits (209), Expect = 1.118e-17 Identity = 55/163 (33.74%), Postives = 92/163 (56.44%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT---TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL-HFNPIPNCELVEILDE 600
VL+ GET GKT+L + + G + +N HE T+ + +G + + +GV++ AMR G +++DE++LA +LE LN + +++R + + + T +++ P F++ AT NP G Y G+K LS+ALRNRF E+ + ELV I D+
Sbjct: 1041 VLMVGETGGGKTTLSEVVGR--GKL-RSINCHERTETADLLGRLRPKQDG----GFEWSDGVVISAMRDGVPLLVDEISLAEDSVLERLNPLFEEDRALLLSDAGTDTEVVESKPGFQMIATMNPGGDY-GKKELSKALRNRFTEVWTSSDYTTSELVTIFDQ 1195 HSP 4 Score: 61.2326 bits (147), Expect = 7.647e-10 Identity = 31/91 (34.07%), Postives = 46/91 (50.55%), Query Frame = 1
Query: 280 TSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
+ ++G +++A GHW+++DE+NLAP + L+A IV + HP FRLFA NP G R+ L +R RF E
Sbjct: 513 AAPFALQKGAVLEAAEKGHWLLVDEINLAPPECLDA------------IVHALSAPGTHPNFRLFACMNPATDAGKRR-LPPGVRTRFTEF 590 HSP 5 Score: 52.373 bits (124), Expect = 4.001e-7 Identity = 26/100 (26.00%), Postives = 56/100 (56.00%), Query Frame = 1
Query: 268 SSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF 558
+++++ + + V V A HG W++++++NL +L+ LN L+ ++ + E Q + ++ HP FR+F + + +SRA+RNR +E++
Sbjct: 1795 ANQSSMRFEWTDSVFVDAYLHGDWLLIEDVNLCSAAVLDRLNSCLESGGKLVVAERQNSYEPLEPHPDFRVFLSMD-----ARVGEISRAMRNRSVEIYI 1889
BLAST of Midasin vs. Ensembl Fly
Match: CG13185 (gene:FBgn0033661 transcript:FBtr0310323) HSP 1 Score: 347.436 bits (890), Expect = 8.431e-108 Identity = 185/352 (52.56%), Postives = 240/352 (68.18%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKC---DRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFR-------VSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y +TESV KNL DLARI+S GK LP+L+QG TS GKTSLI Y+A+ +G+ C R+NNHE TD+QEYIG+Y +++ L F+EGVLVQAMRHG WIILDELNLA TDILEALNRVLDDNRE++I ETQT +KAHP F LFATQNPPGLYGGRK LSRA +NRFIELHF IP EL IL++ C IP RKMV+ M +LQ + + + +LRDLFRW R Y +K D +DW++ ++EEGYL+L+ K RS++E L+ + L FR + D+Q + E + S +++ IL+ +I WT + R+AV+ ++++ DEPVLLVG
Sbjct: 1124 YILTESVKKNLKDLARIISIGK-LPILLQGPTSAGKTSLIDYVARRSGNRCLRINNHEHTDLQEYIGTY----AADLDGKLTFREGVLVQAMRHGFWIILDELNLASTDILEALNRVLDDNRELYIAETQTLVKAHPNFMLFATQNPPGLYGGRKTLSRAFKNRFIELHFADIPREELETILEKRCFIPPTYARKMVQCMTQLQQHRK-----NTSKQVFTLRDLFRWGNR-YTHADKSLHDDNRYDWNQHLVEEGYLVLSSKVRSQEEHELIEKTLFANFRKKLDLQTLFDIQTDRE-----SSSMVSRKILDAIRNFKLRDDIVWTRNMTRMAVVTAKALEFDEPVLLVG 1459 HSP 2 Score: 102.449 bits (254), Expect = 2.616e-23 Identity = 62/184 (33.70%), Postives = 104/184 (56.52%), Query Frame = 1
Query: 85 DLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGS-------YVQQNSSETTS---TLVFKEGVLVQAMRHGH-WIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDE 600
+L R++SA + PVL++G VGKTS++ + G+ R+N E TD+ + G+ ++ N++ T + V+++G L+ A++ + WI+LDELNLAP +LE LN VLD E++I E + R+FA QNP GGRK L ++ NRF +++ + +L+ ++ E
Sbjct: 1782 NLFRLLSALSLQKPVLLEGPPGVGKTSIVESIGSAIGYQIVRINLCEHTDLADLFGTDLPAEDNLLESNTAATERQIRSFVWRDGPLLAALKAKNTWILLDELNLAPQSVLEGLNAVLDHRGEVYIPELNKSFHLAGSTRIFACQNPLKQGGGRKGLPQSFLNRFTKVYLRKLSTEDLLHVVTE 1965 HSP 3 Score: 88.9669 bits (219), Expect = 7.477e-19 Identity = 72/253 (28.46%), Postives = 126/253 (49.80%), Query Frame = 1
Query: 40 VKGYKITESV--SKNLTDLARIVSAGKIL---PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT----------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE-LVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY-----ISLRDLFRWA 735
++ +K+ + + ++N+T +A +V+A + PVL+ G T GKT++ LA + +N H T+ +++G E+T + +G L+ AM+ G + + DE++LA +LE LN +L+ R + + E ++A F+ AT NP G + G+K LS ALRNRF E+ P N E L+EI + + + +A+ ++ +DVV R+ S+RD+ WA
Sbjct: 1423 IRNFKLRDDIVWTRNMTRMA-VVTAKALEFDEPVLLVGPTGCGKTTVCQLLASIADVQLRILNCHMHTEGADFLGGLRPCRGKESTQLFEWADGPLIYAMQEGSYFMADEISLAEDSVLERLNCILEPERTVLLAEKGGISELDASPDFVVQAKSGFQFLATMNPGGDF-GKKELSPALRNRFTEVWCLPSDNKEDLIEI---AYNCMRRQKEHENQAINTHKVAEYLVDVVLHIRDTNDKFKFSVRDILAWA 1670 HSP 4 Score: 66.6254 bits (161), Expect = 1.855e-11 Identity = 35/91 (38.46%), Postives = 52/91 (57.14%), Query Frame = 1
Query: 286 TLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ--TTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
+ F G LV + +G W++LDE+NLA + LE L+ +L+ + ++E T +K HP FR+FA N P G+K L +RNRF E
Sbjct: 886 SFAFIPGSLVNCISNGDWVLLDEINLASAETLECLSTILEPEGSVVLLERGDFTPVKRHPDFRIFACMN-PNTDIGKKDLPVGIRNRFTEF 975
BLAST of Midasin vs. Ensembl Fly
Match: c12.2 (gene:FBgn0040234 transcript:FBtr0334929) HSP 1 Score: 54.299 bits (129), Expect = 1.271e-7 Identity = 64/246 (26.02%), Postives = 105/246 (42.68%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDI-------LEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGL-YGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQE--LDVVHCKRNYISL-RDLFRWARRVYYEHNKCD---RFFDWDRTMIE 801
+L+ G VGK L+ L +L + H T + + ++ + +++ LVQA+R GH +++DE + AP ++ +E+ VL D R I +I+ HP FRL N PG + G + AL + F H NP P E+ + K+P R +V A +L+ + E L+ + R +S+ + L R Y E N + D+DR E
Sbjct: 771 LLLVGNQGVGKNKLVDRLLELMQRPREYIQLHRDTTVHS-----LTLQATLKDGQVSYEDSALVQAVRTGHVLVVDEADKAPVNVTCILRTLVESGEMVLADGRRIVPPGEGGRVPNSIETHPDFRLIVLANRPGFPFLGNDFFA-ALGDVF-SCHAISNPDPESEIFLLQQYGPKVPIKTLRTLVNAFGELRTLADEGMLNYPYSTREVVSIVKHLER-----YPEENMSEVVGNVLDFDRYQPE 1004 HSP 2 Score: 48.9062 bits (115), Expect = 5.787e-6 Identity = 57/244 (23.36%), Postives = 99/244 (40.57%), Query Frame = 1
Query: 88 LARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA------------------------HPMFRLFATQNPPGLYGGRKV-LSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRV 744
LA ++ A + V + GE VGK +L L +L + +E ++ VQQ + V+++ LV+A + G +L+ L+ L R++ D RE+ + + T + A H FR+ A PP L GG+ L+ L + F+ P+ E E+L + C G + + +L SQ+ ++ +S R L + ARR+
Sbjct: 424 LASLLQAYAVGDVCLVGEKGVGKLTLTQELLRLLQQTSEPMMLYEDMTSRDI----VQQRITSPQGDTVWRDSPLVRAAKSGSVAVLNGLHRLHKSTASVLQRLIHD-RELQLCDGTTLLGANRYQALLQQGFSKEELGKLGFLPIHESFRVVALAEPPRLGGGQPSWLTPELLSLFLYQELRPLRQSEEAELLAQLCGGDLPAGMDQLLKLAQLLRSSQD-PLLQGLAGTLSTRQLLKLARRL 661
BLAST of Midasin vs. Ensembl Fly
Match: c12.2 (gene:FBgn0040234 transcript:FBtr0071321) HSP 1 Score: 54.299 bits (129), Expect = 1.337e-7 Identity = 64/246 (26.02%), Postives = 105/246 (42.68%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDI-------LEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGL-YGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQE--LDVVHCKRNYISL-RDLFRWARRVYYEHNKCD---RFFDWDRTMIE 801
+L+ G VGK L+ L +L + H T + + ++ + +++ LVQA+R GH +++DE + AP ++ +E+ VL D R I +I+ HP FRL N PG + G + AL + F H NP P E+ + K+P R +V A +L+ + E L+ + R +S+ + L R Y E N + D+DR E
Sbjct: 754 LLLVGNQGVGKNKLVDRLLELMQRPREYIQLHRDTTVHS-----LTLQATLKDGQVSYEDSALVQAVRTGHVLVVDEADKAPVNVTCILRTLVESGEMVLADGRRIVPPGEGGRVPNSIETHPDFRLIVLANRPGFPFLGNDFFA-ALGDVF-SCHAISNPDPESEIFLLQQYGPKVPIKTLRTLVNAFGELRTLADEGMLNYPYSTREVVSIVKHLER-----YPEENMSEVVGNVLDFDRYQPE 987 HSP 2 Score: 48.9062 bits (115), Expect = 6.088e-6 Identity = 57/244 (23.36%), Postives = 99/244 (40.57%), Query Frame = 1
Query: 88 LARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA------------------------HPMFRLFATQNPPGLYGGRKV-LSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRV 744
LA ++ A + V + GE VGK +L L +L + +E ++ VQQ + V+++ LV+A + G +L+ L+ L R++ D RE+ + + T + A H FR+ A PP L GG+ L+ L + F+ P+ E E+L + C G + + +L SQ+ ++ +S R L + ARR+
Sbjct: 407 LASLLQAYAVGDVCLVGEKGVGKLTLTQELLRLLQQTSEPMMLYEDMTSRDI----VQQRITSPQGDTVWRDSPLVRAAKSGSVAVLNGLHRLHKSTASVLQRLIHD-RELQLCDGTTLLGANRYQALLQQGFSKEELGKLGFLPIHESFRVVALAEPPRLGGGQPSWLTPELLSLFLYQELRPLRQSEEAELLAQLCGGDLPAGMDQLLKLAQLLRSSQD-PLLQGLAGTLSTRQLLKLARRL 644
BLAST of Midasin vs. Ensembl Zebrafish
Match: mdn1 (midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041001-138]) HSP 1 Score: 342.043 bits (876), Expect = 1.011e-105 Identity = 183/351 (52.14%), Postives = 235/351 (66.95%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFR--------VSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL DLAR+V+AG I PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y SS+ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+F+ ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHFN +P+ EL IL + C +P + K+++ M+ LQ + V K YI+LRDLFRWA R Y + + DW + + ++GY+LL G+ R +E + IL + F+ S+ Q+ + FSP ++I + E ++ WT RR+AVL R+++ E VLLVG+
Sbjct: 1032 YILTPSVKLNLKDLARVVAAG-IHPVLLQGETSVGKTSLIKWLAASTGNQCVRINNHEHTDIQEYIGCY----SSDEHGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFVAETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFNELPSSELENILHQRCSLPPSYCSKLIKVMQDLQSLRRGSSVFAGKHGYITLRDLFRWAER-YRLEEQSESSRDWLQHLADDGYMLLAGRVRKPEEAVTIQNILEKHFKKTVNPDALFSEGQVTSQ--FSPFVNSIAGVPEE-------FGHVVWTQGMRRLAVLVGRALRFGESVLLVGD 1367 HSP 2 Score: 112.079 bits (279), Expect = 1.859e-26 Identity = 78/248 (31.45%), Postives = 125/248 (50.40%), Query Frame = 1
Query: 58 TESVSKNLTDLA-----RIVSAGKIL-------PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHP-MFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
+ES ++L+D A V+A +IL P+L++G VGKTSL+ LAK +G+ R+N E TD+ + G+ + + +++G L+ ++ GHWI+LDELNLA +LE LN D EI+I E ++F QNP GGRK L ++ NRF +++ + + N ++ I D I N ++ M +Q ++ + V R + +LRDLFRW +
Sbjct: 1701 SESEGRSLSDYALSAGTTAVNAQRILRALKLQRPILLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFGTDLPVEGGKG-GEFAWRDGPLLAGLKAGHWIVLDELNLASQSVLEGLNACFDHRAEIYIPELGMRFHVQQEKTKIFGCQNPFTQGGGRKGLPKSFLNRFTQVYVDALTNEDMQFIGD---SIFPNIDNAIISKM--VQFSNRLVQEVMVDRKWGQRGGPWEFNLRDLFRWCQ 1942 HSP 3 Score: 90.8929 bits (224), Expect = 2.371e-19 Identity = 65/252 (25.79%), Postives = 123/252 (48.81%), Query Frame = 1
Query: 55 ITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICH----RVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGL-------------YGGRKVLSRALRNRFIELHFNPIPNCELV--EILDESCKIPKNKGRKMVEAMRKLQIESQELD---VVHCKRNYISLRDLFRWARRV 744
+ +S +NL LA V++ K P+L++G GKT+L+ YLA +TGH+ +V + TD + +G Y ++ V++ G L QA+ G+WI+L++++ AP D++ L ++ +N+++ I + I+ P F+ FAT++ Y + +S R ++ PN +V +LD C++ N R + + + +Q + + + +SLRDL +W R+
Sbjct: 291 LVDSTCQNLRRLAMAVASQK--PILLEGPIGCGKTALVEYLAAVTGHVKAPDILKVQLGDQTDSKMLLGMY---RCTDVPGKFVWQPGSLTQAVSKGYWILLEDIDHAPLDVISVLLPLM-ENKKLTIPGREDCIQMSPAFQFFATRSGGAWHRPQNTHAALLDKYWSKLQISNMSREELTQVLIKRFPNLAVVVDRLLDIYCQLTGN--RHQINSSSQGPSAAQSISDDHITSLEGRGLSLRDLLKWCERI 534 HSP 4 Score: 86.6557 bits (213), Expect = 6.549e-18 Identity = 73/249 (29.32%), Postives = 120/249 (48.19%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY-------VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNC--ELVEILDESCKIP------KNKGRKMVEAMRKL--QIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIE 801
VL+ G+T GKT++ A L G +N H + +++G QQ +E + +G L+ AM+ ++DE++LA +LE LN VL+ + + + E + I A FRL AT NP G + G+K LS ALRNRF E+ + P N +L++I+ + + ++G+ + E M + +QE +R +S+RD+ W V + + C+R D TM E
Sbjct: 1362 VLLVGDTGCGKTTICQLFAALAGQKFFSLNCHLHMETSDFLGGLRPVRHTGTQQADAEDGRLFEWHDGPLILAMKQDGVFLMDEISLADDSVLERLNSVLEPEKSLVLAEKGSGDEEVELIIAGNKFRLVATMNPGGDF-GKKELSPALRNRFTEI-WCPQSNSRNDLIQIIQHNLRPGLSLDQHDHQGKDIAELMLDFIDWLTNQEFG----RRCILSVRDILSW---VNFMNTVCERDEDGFMTMGE 1601 HSP 5 Score: 68.1662 bits (165), Expect = 6.517e-12 Identity = 48/145 (33.10%), Postives = 69/145 (47.59%), Query Frame = 1
Query: 175 LAKLTGHICHRVNNHETTD------IQEYIGSYV--------QQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELH 555
L KL H+C E D +QE + Q + E+ F EG L QA++ G WI+LDE+NLA + LE L+ +L+ + ++ +T+ + H FRLFA NP G R L +RNRF EL+
Sbjct: 741 LLKLMDHVCKSALTKELHDKPNAALLQEQWEALALKLSQTQEQIRACESAMVFAFVEGTLAQAVKKGEWILLDEINLAAAETLECLSGLLEGSSGSLVLLDRGDTEPLFR-HSDFRLFACMNPATDVGKRN-LPLGIRNRFTELY 883 HSP 6 Score: 61.6178 bits (148), Expect = 7.933e-10 Identity = 33/92 (35.87%), Postives = 51/92 (55.43%), Query Frame = 1
Query: 301 EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF 558
+G LVQA++ G W+++D +N +L+ LN +L+ + I E T TI HP FRLF T +P +SRA+RNR +E++
Sbjct: 2213 DGTLVQALQAGDWLLMDNVNFCSPSVLDRLNALLEPGGSLTINERGIIDGTTPTITPHPNFRLFLTMDPT-----HGEISRAMRNRGVEIYI 2299 HSP 7 Score: 50.0618 bits (118), Expect = 3.797e-6 Identity = 27/69 (39.13%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S L L+ V+ G+ P+L+ GET GKTS + YLA LTGH VN ++ +D + +G Y
Sbjct: 627 FAATRPSSVLLEQLSVCVTKGE--PILLVGETGTGKTSTVQYLAGLTGHRLRVVNMNQQSDTADLLGGY 693
BLAST of Midasin vs. Ensembl Zebrafish
Match: vwa8 (von Willebrand factor A domain containing 8 [Source:NCBI gene;Acc:561317]) HSP 1 Score: 48.9062 bits (115), Expect = 8.313e-6 Identity = 49/193 (25.39%), Postives = 79/193 (40.93%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTD---ILEAL----NRVLDDNREIF-----IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
+L+ G VGK ++ L + H T +Q + S +++++ LV+A++ GH +++DE + APT+ IL+AL +L D R I I HP FR+ N PG AL + F H NP P E + +P +K+V A +L+
Sbjct: 776 LLLVGNQGVGKNKIVDRFLHLMNRPREYLQLHRDTTVQT-----LTLQPSVRDGIIIYEDSPLVKAVKMGHILVIDEADKAPTNVTCILKALVESGEMILADGRRIVSDALEAAGRPNAIPMHPDFRMIVLANRPGFPFLGNDFFGALGDIF-SCHAVDNPKPQAEFAMLKQYGPDVPDAVLQKLVAAFGELR 962
BLAST of Midasin vs. Ensembl Zebrafish
Match: vwa8 (von Willebrand factor A domain containing 8 [Source:NCBI gene;Acc:561317]) HSP 1 Score: 48.9062 bits (115), Expect = 8.386e-6 Identity = 49/193 (25.39%), Postives = 79/193 (40.93%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTD---ILEAL----NRVLDDNREIF-----IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
+L+ G VGK ++ L + H T +Q + S +++++ LV+A++ GH +++DE + APT+ IL+AL +L D R I I HP FR+ N PG AL + F H NP P E + +P +K+V A +L+
Sbjct: 776 LLLVGNQGVGKNKIVDRFLHLMNRPREYLQLHRDTTVQT-----LTLQPSVRDGIIIYEDSPLVKAVKMGHILVIDEADKAPTNVTCILKALVESGEMILADGRRIVSDALEAAGRPNAIPMHPDFRMIVLANRPGFPFLGNDFFGALGDIF-SCHAVDNPKPQAEFAMLKQYGPDVPDAVLQKLVAAFGELR 962
BLAST of Midasin vs. Ensembl Zebrafish
Match: vwa8 (von Willebrand factor A domain containing 8 [Source:NCBI gene;Acc:561317]) HSP 1 Score: 48.9062 bits (115), Expect = 8.605e-6 Identity = 49/193 (25.39%), Postives = 79/193 (40.93%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTD---ILEAL----NRVLDDNREIF-----IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
+L+ G VGK ++ L + H T +Q + S +++++ LV+A++ GH +++DE + APT+ IL+AL +L D R I I HP FR+ N PG AL + F H NP P E + +P +K+V A +L+
Sbjct: 768 LLLVGNQGVGKNKIVDRFLHLMNRPREYLQLHRDTTVQT-----LTLQPSVRDGIIIYEDSPLVKAVKMGHILVIDEADKAPTNVTCILKALVESGEMILADGRRIVSDALEAAGRPNAIPMHPDFRMIVLANRPGFPFLGNDFFGALGDIF-SCHAVDNPKPQAEFAMLKQYGPDVPDAVLQKLVAAFGELR 954
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293]) HSP 1 Score: 336.65 bits (862), Expect = 8.330e-104 Identity = 177/347 (51.01%), Postives = 236/347 (68.01%), Query Frame = 1
Query: 43 KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVN--IANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
+ Y +T SV+ NL DL R+VSAG PVL+QGETSVGKTSLI +LA +G+ C R+NNHE TDIQEYIG Y +S+ + L+FKEGVL+ AMR G+W+ILDELNLAPTD+LEALNR+LDDNRE+FI ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V M +LQ + V K +I+LRDLFRWA R Y + ++ +DW + + +G++LL G+ R +E ++ ++L + F+ Q+ + FS L +V+ ++I WT RR+AVL R++Q EPVLLVG+
Sbjct: 1002 ESYILTYSVNLNLRDLVRVVSAG-THPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCY----TSDVSGKLIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSAELETILHKRCCLPPSYCNKLVAVMLELQSYRRGSSVFAGKHGFITLRDLFRWAER-YRLSEQNEKDYDWLQHLANDGFMLLAGRVRKPEEEEVILKVLKKCFK---KQVEPQHIFSRGSVQRQLDSLSVQQDIVDFRFSHIVWTQGMRRLAVLVGRALQFGEPVLLVGD 1339 HSP 2 Score: 114.39 bits (285), Expect = 4.648e-27 Identity = 74/234 (31.62%), Postives = 117/234 (50.00%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY------ISLRDLFRWAR 738
E+ S N L R + K P++++G VGKTSL+T LAK +G+ R+N E TD+ + G+ + + +++G L+ A++ GHWI+LDELNLA +LE LN D E++I E + H ++F QNP GGRK L R+ NRF +++ + + ++ I + + + G V M + +V K +LRDLFRW +
Sbjct: 1677 AETTSMNAQRLLRALQLNK--PIILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKG-GEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTKIFGCQNPFHQGGGRKGLPRSFLNRFTQVYVDQLSFADMEFIAN---SLFPSLGTSTVSKMVAFNNKIDHQVMVEKKWGQKGGPWEFNLRDLFRWCQ 1904 HSP 3 Score: 90.5077 bits (223), Expect = 4.871e-19 Identity = 80/267 (29.96%), Postives = 123/267 (46.07%), Query Frame = 1
Query: 55 ITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICH------RVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQ-----------NPPGLYGG-------RKVLSRALRNRFIELHFNPIPNCELV--EILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---------ISLRDLFRWARRVYY 750
+ ES KNL LA VS PVL++G GKT+L+ YLA +TG H +V + TD + +G Y ++ V++ G L QA+ +GHWI+L++++ AP D++ L VL +N E+ + IK P F+LFAT+ P + R L R E+ PN ELV +LD ++ +K AM L+ E++ L ++N +SLRDL +W R+ +
Sbjct: 288 LVESTCKNLHSLALAVSYQN--PVLLEGPIGCGKTALVEYLATVTGR--HQPPDILKVQLGDQTDSKMLLGMY---RCTDIPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVL-ENGELLMPGRGDCIKVAPGFQLFATRRLLSSSSGVWYRPQNTHASLLDKHWTRIHLGNMSREELKEVILKLYPNLELVTDRLLDIYIQLTGDKHL----AMSDLETEAEALLQKPLEQNVPRPNLEGRGLSLRDLLKWCNRIIH 542 HSP 4 Score: 82.8037 bits (203), Expect = 1.467e-16 Identity = 52/160 (32.50%), Postives = 84/160 (52.50%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSS-----ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT---------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
PVL+ G+T GKT++ A L H VN H+ + +++G ++Q S E + + +G LV A++ + ++DE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1333 PVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKDDVEYSKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKGSGEDETEEVEILTAGKQFRILATMNPGGDF-GKKELSPALRNRFTEI 1491 HSP 5 Score: 72.0182 bits (175), Expect = 5.419e-13 Identity = 58/179 (32.40%), Postives = 84/179 (46.93%), Query Frame = 1
Query: 259 QQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQI-----ESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCD 768
Q +E F EG L QA++ G WI+LDE+NLA + LE L +L+ ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + N ++IL N + +V+ + + S EL R + SLR L R R Y N C+
Sbjct: 754 QMRMTENAVLFAFVEGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELENEGDLQILIMDYLRGLNVSKTVVQGIISFYLAIRKKSSAELVDGTGHRPHYSLRTLCRALR--YASANPCN 928 HSP 6 Score: 66.6254 bits (161), Expect = 2.730e-11 Identity = 50/173 (28.90%), Postives = 86/173 (49.71%), Query Frame = 1
Query: 136 GETSVGKTSLITYLAKLTGHICH--RVNNHETTDIQEYIGSYVQQNSS-----------ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD 597
GE G T+ + L KL G + R+N T + G+ V+Q S +++ T + +G+LVQA++ G W+++D +N + +L+ LN +L+ + + E + TI HP FRLF + +P +SRA+RNR IEL+ + L+++ D
Sbjct: 2111 GEAGSGITTDL--LNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSMLTITPHPNFRLFLSMDP-----SHGEISRAMRNRGIELYISGESEGMLLDLFD 2276
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293]) HSP 1 Score: 336.65 bits (862), Expect = 9.340e-104 Identity = 177/347 (51.01%), Postives = 236/347 (68.01%), Query Frame = 1
Query: 43 KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVN--IANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
+ Y +T SV+ NL DL R+VSAG PVL+QGETSVGKTSLI +LA +G+ C R+NNHE TDIQEYIG Y +S+ + L+FKEGVL+ AMR G+W+ILDELNLAPTD+LEALNR+LDDNRE+FI ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V M +LQ + V K +I+LRDLFRWA R Y + ++ +DW + + +G++LL G+ R +E ++ ++L + F+ Q+ + FS L +V+ ++I WT RR+AVL R++Q EPVLLVG+
Sbjct: 968 ESYILTYSVNLNLRDLVRVVSAG-THPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCY----TSDVSGKLIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSAELETILHKRCCLPPSYCNKLVAVMLELQSYRRGSSVFAGKHGFITLRDLFRWAER-YRLSEQNEKDYDWLQHLANDGFMLLAGRVRKPEEEEVILKVLKKCFK---KQVEPQHIFSRGSVQRQLDSLSVQQDIVDFRFSHIVWTQGMRRLAVLVGRALQFGEPVLLVGD 1305 HSP 2 Score: 114.39 bits (285), Expect = 5.050e-27 Identity = 74/234 (31.62%), Postives = 117/234 (50.00%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY------ISLRDLFRWAR 738
E+ S N L R + K P++++G VGKTSL+T LAK +G+ R+N E TD+ + G+ + + +++G L+ A++ GHWI+LDELNLA +LE LN D E++I E + H ++F QNP GGRK L R+ NRF +++ + + ++ I + + + G V M + +V K +LRDLFRW +
Sbjct: 1643 AETTSMNAQRLLRALQLNK--PIILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKG-GEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTKIFGCQNPFHQGGGRKGLPRSFLNRFTQVYVDQLSFADMEFIAN---SLFPSLGTSTVSKMVAFNNKIDHQVMVEKKWGQKGGPWEFNLRDLFRWCQ 1870 HSP 3 Score: 90.5077 bits (223), Expect = 4.821e-19 Identity = 79/267 (29.59%), Postives = 127/267 (47.57%), Query Frame = 1
Query: 55 ITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICH------RVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQ----NPPGL-YGGRKVLSRALRNRFIELHFNPI-------------PNCELV--EILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---------ISLRDLFRWARRVYY 750
+ ES KNL LA VS PVL++G GKT+L+ YLA +TG H +V + TD + +G Y ++ V++ G L QA+ +GHWI+L++++ AP D++ L VL +N E+ + IK P F+LFAT+ + G+ Y + + L + +H + PN ELV +LD ++ +K AM L+ E++ L ++N +SLRDL +W R+ +
Sbjct: 281 LVESTCKNLHSLALAVSYQN--PVLLEGPIGCGKTALVEYLATVTGR--HQPPDILKVQLGDQTDSKMLLGMY---RCTDIPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVL-ENGELLMPGRGDCIKVAPGFQLFATRRLLSSSSGVWYRPQNTHASLLDKHWTRIHLGNMSREELKEVILKLYPNLELVTDRLLDIYIQLTGDKHL----AMSDLETEAEALLQKPLEQNVPRPNLEGRGLSLRDLLKWCNRIIH 535 HSP 4 Score: 82.8037 bits (203), Expect = 1.664e-16 Identity = 52/160 (32.50%), Postives = 84/160 (52.50%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSS-----ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT---------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
PVL+ G+T GKT++ A L H VN H+ + +++G ++Q S E + + +G LV A++ + ++DE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1299 PVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKDDVEYSKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKGSGEDETEEVEILTAGKQFRILATMNPGGDF-GKKELSPALRNRFTEI 1457 HSP 5 Score: 72.0182 bits (175), Expect = 5.869e-13 Identity = 83/305 (27.21%), Postives = 121/305 (39.67%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLT------------------------GHI--CHRVN-----------------NHETTDIQEYI-------------GSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQI-----ESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCD 768
+ T S + LA V+ G+ VL+ GET GKTS + YL+ + GHI C+R N T E++ + Q +E F EG L QA++ G WI+LDE+NLA + LE L +L+ ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + N ++IL N + +V+ + + S EL R + SLR L R R Y N C+
Sbjct: 596 FAATRPSSVLIEQLAVCVNKGE--SVLLVGETGTGKTSTVQYLSHVAVTADEGFEELFAQTFSRKQNQTFLGHIQTCYRQKRWQDLLKLMQHVLRSALNKVQTGEAEFLLKEKWEGFSLRLNQAQQQMRMTENAVLFAFVEGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELENEGDLQILIMDYLRGLNVSKTVVQGIISFYLAIRKKSSAELVDGTGHRPHYSLRTLCRALR--YASANPCN 894 HSP 6 Score: 66.6254 bits (161), Expect = 3.008e-11 Identity = 50/173 (28.90%), Postives = 85/173 (49.13%), Query Frame = 1
Query: 136 GETSVGKTSLITYLAKLTGHICH--RVNNHETTDIQEYIGSYVQQNSS-----------ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD 597
GE G T+ L KL G + R+N T + G+ V+Q S +++ T + +G+LVQA++ G W+++D +N + +L+ LN +L+ + + E + TI HP FRLF + +P +SRA+RNR IEL+ + L+++ D
Sbjct: 2077 GEAGSGITT--DLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSMLTITPHPNFRLFLSMDP-----SHGEISRAMRNRGIELYISGESEGMLLDLFD 2242
BLAST of Midasin vs. Ensembl Xenopus
Match: MDN1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:100486293]) HSP 1 Score: 335.88 bits (860), Expect = 1.769e-103 Identity = 177/347 (51.01%), Postives = 236/347 (68.01%), Query Frame = 1
Query: 43 KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVN--IANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
+ Y +T SV+ NL DL R+VSAG PVL+QGETSVGKTSLI +LA +G+ C R+NNHE TDIQEYIG Y +S+ + L+FKEGVL+ AMR G+W+ILDELNLAPTD+LEALNR+LDDNRE+FI ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V M +LQ + V K +I+LRDLFRWA R Y + ++ +DW + + +G++LL G+ R +E ++ ++L + F+ Q+ + FS L +V+ ++I WT RR+AVL R++Q EPVLLVG+
Sbjct: 1002 ESYILTYSVNLNLRDLVRVVSAG-THPVLIQGETSVGKTSLIRWLACASGNHCVRINNHEHTDIQEYIGCY----TSDVSGKLIFKEGVLIDAMRKGYWVILDELNLAPTDVLEALNRLLDDNRELFITETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSAELETILHKRCCLPPSYCNKLVAVMLELQSYRRGSSVFAGKHGFITLRDLFRWAER-YRLSEQNEKDYDWLQHLANDGFMLLAGRVRKPEEEEVILKVLKKCFK---KQVEPQHIFSRGSVQRQLDSLSVQQDIVDFRFSHIVWTQGMRRLAVLVGRALQFGEPVLLVGD 1339 HSP 2 Score: 114.775 bits (286), Expect = 4.005e-27 Identity = 74/234 (31.62%), Postives = 117/234 (50.00%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY------ISLRDLFRWAR 738
E+ S N L R + K P++++G VGKTSL+T LAK +G+ R+N E TD+ + G+ + + +++G L+ A++ GHWI+LDELNLA +LE LN D E++I E + H ++F QNP GGRK L R+ NRF +++ + + ++ I + + + G V M + +V K +LRDLFRW +
Sbjct: 1664 AETTSMNAQRLLRALQLNK--PIILEGSPGVGKTSLVTALAKASGNCLVRINLSEQTDVTDLFGTDLPVEGGKG-GEFAWRDGPLLSALKAGHWIVLDELNLASQSVLEGLNACFDHRAEVYIPELGMNFQVQHERTKIFGCQNPFHQGGGRKGLPRSFLNRFTQVYVDQLSFADMEFIAN---SLFPSLGTSTVSKMVAFNNKIDHQVMVEKKWGQKGGPWEFNLRDLFRWCQ 1891 HSP 3 Score: 90.5077 bits (223), Expect = 4.134e-19 Identity = 79/267 (29.59%), Postives = 127/267 (47.57%), Query Frame = 1
Query: 55 ITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICH------RVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQ----NPPGL-YGGRKVLSRALRNRFIELHFNPI-------------PNCELV--EILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---------ISLRDLFRWARRVYY 750
+ ES KNL LA VS PVL++G GKT+L+ YLA +TG H +V + TD + +G Y ++ V++ G L QA+ +GHWI+L++++ AP D++ L VL +N E+ + IK P F+LFAT+ + G+ Y + + L + +H + PN ELV +LD ++ +K AM L+ E++ L ++N +SLRDL +W R+ +
Sbjct: 288 LVESTCKNLHSLALAVSYQN--PVLLEGPIGCGKTALVEYLATVTGR--HQPPDILKVQLGDQTDSKMLLGMY---RCTDIPGEFVWQPGTLTQAVANGHWILLEDIDYAPLDVISVLLPVL-ENGELLMPGRGDCIKVAPGFQLFATRRLLSSSSGVWYRPQNTHASLLDKHWTRIHLGNMSREELKEVILKLYPNLELVTDRLLDIYIQLTGDKHL----AMSDLETEAEALLQKPLEQNVPRPNLEGRGLSLRDLLKWCNRIIH 542 HSP 4 Score: 86.6557 bits (213), Expect = 8.437e-18 Identity = 62/217 (28.57%), Postives = 105/217 (48.39%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT---------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPN--CELVEILDESCKIPKNKGRKMVEAMRKL--QIESQELDVVHCKRNYISLRDLFRW 732
PVL+ G+T GKT++ A L H VN H+ + +++G + +G LV A++ + ++DE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+ + P N +L EI+ + + G + + M + +Q+ +R +S+RD+ W
Sbjct: 1333 PVLLVGDTGCGKTTICQLFAALASQTLHCVNCHQHMETSDFLGGLRPIRQRSKDKLFEWHDGPLVLAIKEDGFFLMDEISLADDSVLERLNSVLEVEKTLVLAEKGSGEDETEEVEILTAGKQFRILATMNPGGDF-GKKELSPALRNRFTEI-WCPQSNDRHDLAEIIKHNLHTGLSLGADIADLMMDFIEWLTNQDFG----RRCILSVRDILSW 1543 HSP 5 Score: 72.7886 bits (177), Expect = 3.285e-13 Identity = 65/218 (29.82%), Postives = 99/218 (45.41%), Query Frame = 1
Query: 259 QQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQI-----ESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEE---GYLLLTGKARSKDEVNLVARILAE 876
Q +E F EG L QA++ G WI+LDE+NLA + LE L +L+ ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + N ++IL N + +V+ + + S EL R + SLR L R R Y N C+ R++ E G+L +A + L+ R + +
Sbjct: 754 QMRMTENAVLFAFVEGTLAQAVKKGEWILLDEINLAAAETLECLCGLLEGISGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELENEGDLQILIMDYLRGLNVSKTVVQGIISFYLAIRKKSSAELVDGTGHRPHYSLRTLCRALR--YASANPCNSV---QRSLYEGFCLGFLTQLDRASHSVVLGLIRRYIVQ 964 HSP 6 Score: 67.0106 bits (162), Expect = 2.192e-11 Identity = 50/173 (28.90%), Postives = 85/173 (49.13%), Query Frame = 1
Query: 136 GETSVGKTSLITYLAKLTGHICH--RVNNHETTDIQEYIGSYVQQNSS-----------ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD 597
GE G T+ L KL G + R+N T + G+ V+Q S +++ T + +G+LVQA++ G W+++D +N + +L+ LN +L+ + + E + TI HP FRLF + +P +SRA+RNR IEL+ + L+++ D
Sbjct: 2098 GEAGSGITT--DLLNKLEGVLVQIQRLNLRLNTFTKTEFGALVEQFHSFKEQLLHSVEGQSSGTFEWVDGMLVQALQCGDWLLMDNVNFCSSSVLDRLNALLEPGGVLTMTERGVTDGSMLTITPHPNFRLFLSMDP-----SHGEISRAMRNRGIELYISGESEGMLLDLFD 2263
BLAST of Midasin vs. Ensembl Xenopus
Match: cybc1 (cytochrome b-245 chaperone 1 [Source:Xenbase;Acc:XB-GENE-977743]) HSP 1 Score: 50.0618 bits (118), Expect = 4.887e-6 Identity = 46/193 (23.83%), Postives = 80/193 (41.45%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDI-------LEALNRVLDDNREIF-----IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
+L+ G VGK ++ L + H T +Q + S +V+++ LV+A + GH +++DE + APT++ +E+ +L D R + + I HP F++ N PG AL + F H NP+P EL + +P +K+V A +L+
Sbjct: 770 LLLVGNQGVGKNKIVDRFLHLLNRPREYLQLHRDTTVQS-----LTLQPSVKEGVIVYEDSPLVKACKKGHILVIDEADKAPTNVTCILKTLVESGEMILSDGRRLVANLSKLDRRPNLIAIHPDFKMIVLANRPGFPFLGNDFFGALGDIF-SCHAVDNPMPQSELAMLKQYGPNVPDAVLQKLVAAFGELR 956
BLAST of Midasin vs. Ensembl Xenopus
Match: cybc1 (cytochrome b-245 chaperone 1 [Source:Xenbase;Acc:XB-GENE-977743]) HSP 1 Score: 50.0618 bits (118), Expect = 5.299e-6 Identity = 46/193 (23.83%), Postives = 80/193 (41.45%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDI-------LEALNRVLDDNREIF-----IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
+L+ G VGK ++ L + H T +Q + S +V+++ LV+A + GH +++DE + APT++ +E+ +L D R + + I HP F++ N PG AL + F H NP+P EL + +P +K+V A +L+
Sbjct: 766 LLLVGNQGVGKNKIVDRFLHLLNRPREYLQLHRDTTVQS-----LTLQPSVKEGVIVYEDSPLVKACKKGHILVIDEADKAPTNVTCILKTLVESGEMILSDGRRLVANLSKLDRRPNLIAIHPDFKMIVLANRPGFPFLGNDFFGALGDIF-SCHAVDNPMPQSELAMLKQYGPNVPDAVLQKLVAAFGELR 952
BLAST of Midasin vs. Ensembl Mouse
Match: Mdn1 (midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:1926159]) HSP 1 Score: 340.887 bits (873), Expect = 2.384e-105 Identity = 175/347 (50.43%), Postives = 238/347 (68.59%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLV----NIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+AR+VSAG PVL+QGETSVGKTSLI +LA +G+ C R+NNHE TDIQEYIG Y +S+T+ LVF EGVL+ AMR G+WI+LDELNLAPTD+LEALNR+LDDNRE+ I ETQ ++AHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y + +DW + + +G++LL G+ R ++E +++ +L + F+ +L + FS N+ K++ + +Q +++ WT RR+AVL R+++ EPVLLVG+
Sbjct: 1052 YVLTPSVKLNLRDIARVVSAG-TYPVLIQGETSVGKTSLIRWLAAASGNHCVRINNHEHTDIQEYIGCY----TSDTSGKLVFNEGVLIDAMRKGYWIVLDELNLAPTDVLEALNRLLDDNRELLITETQEVVRAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRRSSVFAGKQGFIALRDLFRWAER-YRLAEQTQEDYDWLQHLANDGFMLLAGRVRKQEEADVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKSSTQTTVLESKFSHVVWTESMRRLAVLVGRALEFGEPVLLVGD 1387 HSP 2 Score: 115.546 bits (288), Expect = 1.411e-27 Identity = 93/339 (27.43%), Postives = 164/339 (48.38%), Query Frame = 1
Query: 94 RIVSAGKI-LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWARRVYYEHNK-CDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQL--NDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVL 1065
R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +G L+ A++ GHW++LDELNLA ILE LN D EI++ E + + H R+F QNP GGRK L ++ NRF ++ +P+ ++ E + + I K+ +KMV + E V ++ + +LRDLFRW + + + + C +D + + +L+ + R++++ V + +VF + + ++ +P I +L + S + + + RR L +S Q EP++
Sbjct: 1733 RLLRAAKLNKPILLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFGADLPVEGGRG-GEFAWCDGPLLAALKAGHWVVLDELNLASQSILEGLNACFDHRGEIYVPELGMSFQVQHEKTRIFGCQNPFRQGGGRKGLPKSFLNRFTQVFVDPLTVVDM-EFIASTLFPTIDKDIVKKMVAFNNHIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQLILVDQSPGC---YDPGQHV----FLVYGERMRTREDKEKVVAVFKDVFASNSMPYIGTRQFHITPYDIQIGYSVLSRSSYVPHPS--------RRPLSLLHQSFQSLEPIM 2048 HSP 3 Score: 81.2629 bits (199), Expect = 4.398e-16 Identity = 72/249 (28.92%), Postives = 122/249 (49.00%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQ--NSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE-LVEILDESCKIP------KNKGRKMVEAMRKL--QIESQELDVVHCKRNYISLRDLFRW 732
TES+ + + R + G+ PVL+ G+T GKT++ + L + VN H + +++G V+Q N + T +F+ +G LV AM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+ E L++I++ + + +KG + E M + QE ++ +S+RD+ W
Sbjct: 1362 TESMRRLAVLVGRALEFGE--PVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLGGLRPVRQKPNDKDELDTRLFEWHDGPLVLAMKEDSFFLLDEISLADDSVLERLNSVLEVEKCLVLAEKGSPESKDNEVELLTAGKHFRILATMNPGGDF-GKKELSPALRNRFTEIWCPQSTKREDLIQIINHNLRPGLSLAGVGHKGAGVAEVMLAFITWLTRQEFG----RKCVVSIRDILSW 1603 HSP 4 Score: 77.411 bits (189), Expect = 7.861e-15 Identity = 52/157 (33.12%), Postives = 83/157 (52.87%), Query Frame = 1
Query: 25 ETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQ 483
E + +K + + ESV KNL LA VS VL++G GKTSL+ +LA +TG +V + TD + +G Y ++ V++ G L QA G+WI+L++++ AP D++ L +L+ E+ I +K P F+LFAT+
Sbjct: 294 EQELALKSFVLVESVCKNLQLLA--VSVASQNAVLLEGPIGSGKTSLVEHLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATKGYWILLEDIDYAPLDVVSVLIPLLEHG-ELLIPGHGDCLKVAPTFQLFATR 444 HSP 5 Score: 75.8702 bits (185), Expect = 2.223e-14 Identity = 61/183 (33.33%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T + L+F EG L QA++ G WI+LDE+NLA + LE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E ++IL + + KN + +V L+ ES +L R + SLR L R R + N C
Sbjct: 797 NHAQQQMKMTENALLFAFVEGTLAQAIKKGEWILLDEINLAAPETLECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQILIVDYLKGLNVSKNTVQGIVNFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 975 HSP 6 Score: 56.6102 bits (135), Expect = 4.003e-8 Identity = 33/133 (24.81%), Postives = 71/133 (53.38%), Query Frame = 1
Query: 202 HRVNNHETTDIQEYIGSY-------VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
+++N++ D + + + +Q S + + + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + T+ +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2181 NKINSYSKADFAKLVEDFRSFGVKLLQSASGRSHGSFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTINERGMVDGSTCTVTPNPNFRLFLSMDP--IHGE---ISRAMRNRGLEIYIS 2308
BLAST of Midasin vs. Ensembl Mouse
Match: Mdn1 (midasin AAA ATPase 1 [Source:MGI Symbol;Acc:MGI:1926159]) HSP 1 Score: 340.887 bits (873), Expect = 2.600e-105 Identity = 175/347 (50.43%), Postives = 238/347 (68.59%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLV----NIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+AR+VSAG PVL+QGETSVGKTSLI +LA +G+ C R+NNHE TDIQEYIG Y +S+T+ LVF EGVL+ AMR G+WI+LDELNLAPTD+LEALNR+LDDNRE+ I ETQ ++AHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y + +DW + + +G++LL G+ R ++E +++ +L + F+ +L + FS N+ K++ + +Q +++ WT RR+AVL R+++ EPVLLVG+
Sbjct: 1052 YVLTPSVKLNLRDIARVVSAG-TYPVLIQGETSVGKTSLIRWLAAASGNHCVRINNHEHTDIQEYIGCY----TSDTSGKLVFNEGVLIDAMRKGYWIVLDELNLAPTDVLEALNRLLDDNRELLITETQEVVRAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRRSSVFAGKQGFIALRDLFRWAER-YRLAEQTQEDYDWLQHLANDGFMLLAGRVRKQEEADVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKSSTQTTVLESKFSHVVWTESMRRLAVLVGRALEFGEPVLLVGD 1387 HSP 2 Score: 115.546 bits (288), Expect = 1.492e-27 Identity = 93/339 (27.43%), Postives = 164/339 (48.38%), Query Frame = 1
Query: 94 RIVSAGKI-LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWARRVYYEHNK-CDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQL--NDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVL 1065
R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +G L+ A++ GHW++LDELNLA ILE LN D EI++ E + + H R+F QNP GGRK L ++ NRF ++ +P+ ++ E + + I K+ +KMV + E V ++ + +LRDLFRW + + + + C +D + + +L+ + R++++ V + +VF + + ++ +P I +L + S + + + RR L +S Q EP++
Sbjct: 1733 RLLRAAKLNKPILLEGSPGVGKTSLVAALAKASGNTLVRINLSEQTDITDLFGADLPVEGGRG-GEFAWCDGPLLAALKAGHWVVLDELNLASQSILEGLNACFDHRGEIYVPELGMSFQVQHEKTRIFGCQNPFRQGGGRKGLPKSFLNRFTQVFVDPLTVVDM-EFIASTLFPTIDKDIVKKMVAFNNHIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQLILVDQSPGC---YDPGQHV----FLVYGERMRTREDKEKVVAVFKDVFASNSMPYIGTRQFHITPYDIQIGYSVLSRSSYVPHPS--------RRPLSLLHQSFQSLEPIM 2048 HSP 3 Score: 80.8777 bits (198), Expect = 4.905e-16 Identity = 72/249 (28.92%), Postives = 122/249 (49.00%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQ--NSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE-LVEILDESCKIP------KNKGRKMVEAMRKL--QIESQELDVVHCKRNYISLRDLFRW 732
TES+ + + R + G+ PVL+ G+T GKT++ + L + VN H + +++G V+Q N + T +F+ +G LV AM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+ E L++I++ + + +KG + E M + QE ++ +S+RD+ W
Sbjct: 1362 TESMRRLAVLVGRALEFGE--PVLLVGDTGCGKTTVCQMFSALANQKLYSVNCHLNMETSDFLGGLRPVRQKPNDKDELDTRLFEWHDGPLVLAMKEDSFFLLDEISLADDSVLERLNSVLEVEKCLVLAEKGSPESKDNEVELLTAGKHFRILATMNPGGDF-GKKELSPALRNRFTEIWCPQSTKREDLIQIINHNLRPGLSLAGVGHKGAGVAEVMLAFITWLTRQEFG----RKCVVSIRDILSW 1603 HSP 4 Score: 77.411 bits (189), Expect = 8.152e-15 Identity = 52/157 (33.12%), Postives = 83/157 (52.87%), Query Frame = 1
Query: 25 ETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQ 483
E + +K + + ESV KNL LA VS VL++G GKTSL+ +LA +TG +V + TD + +G Y ++ V++ G L QA G+WI+L++++ AP D++ L +L+ E+ I +K P F+LFAT+
Sbjct: 294 EQELALKSFVLVESVCKNLQLLA--VSVASQNAVLLEGPIGSGKTSLVEHLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATKGYWILLEDIDYAPLDVVSVLIPLLEHG-ELLIPGHGDCLKVAPTFQLFATR 444 HSP 5 Score: 75.8702 bits (185), Expect = 2.411e-14 Identity = 61/183 (33.33%), Postives = 94/183 (51.37%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T + L+F EG L QA++ G WI+LDE+NLA + LE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E ++IL + + KN + +V L+ ES +L R + SLR L R R + N C
Sbjct: 797 NHAQQQMKMTENALLFAFVEGTLAQAIKKGEWILLDEINLAAPETLECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQILIVDYLKGLNVSKNTVQGIVNFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 975 HSP 6 Score: 56.6102 bits (135), Expect = 4.183e-8 Identity = 33/133 (24.81%), Postives = 71/133 (53.38%), Query Frame = 1
Query: 202 HRVNNHETTDIQEYIGSY-------VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
+++N++ D + + + +Q S + + + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + T+ +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2181 NKINSYSKADFAKLVEDFRSFGVKLLQSASGRSHGSFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTINERGMVDGSTCTVTPNPNFRLFLSMDP--IHGE---ISRAMRNRGLEIYIS 2308
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q9NU22|MDN1_HUMAN (Midasin OS=Homo sapiens OX=9606 GN=MDN1 PE=1 SV=2) HSP 1 Score: 346.665 bits (888), Expect = 1.812e-106 Identity = 178/347 (51.30%), Postives = 241/347 (69.45%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQL----VNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL D+ R+VSAG PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y +S+++ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+ + ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K+ +I+LRDLFRWA R Y ++ +DW + + +GY+LL G+ R ++E++++ +L + F+ +L + FS N+ K++ + +Q+ N +I WT RR+A+L R+++ EPVLLVG+
Sbjct: 1056 YILTSSVKLNLRDIVRVVSAG-TYPVLIQGETSVGKTSLIQWLAAATGNHCVRINNHEHTDIQEYIGCY----TSDSSGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELLVTETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSSELETILHKRCSLPPSYCSKLVKVMLDLQSYRRSSSVFAGKQGFITLRDLFRWAER-YRLAEPTEKEYDWLQHLANDGYMLLAGRVRKQEEIDVIQEVLEKHFK---KKLCPQSLFSKE--NVLKLLGKLSTQISTLECNFGHIVWTEGMRRLAMLVGRALEFGEPVLLVGD 1391 HSP 2 Score: 115.931 bits (289), Expect = 7.383e-27 Identity = 76/237 (32.07%), Postives = 123/237 (51.90%), Query Frame = 1
Query: 64 SVSKNLTDLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
S + R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TDI + G+ + + +++G L+ A++ GHW++LDELNLA +LE LN D EI++ E + + H ++F QNP GGRK L R+ NRF ++ +P+ ++ E + + I KN +KMV ++ E V ++ + +LRDLFRW +
Sbjct: 1728 SAGTTAMNAQRLLRATKLKKPILLEGSPGVGKTSLVGALAKASGNTLVRINLSEQTDITDLFGADLPVEGGKG-GEFAWRDGPLLAALKAGHWVVLDELNLASQSVLEGLNACFDHRGEIYVPELGMSFQVQHEKTKIFGCQNPFRQGGGRKGLPRSFLNRFTQVFVDPLTVIDM-EFIASTLFPAIEKNIVKKMVAFNNQIDHE------VTVEKKWGQKGGPWEFNLRDLFRWCQ 1956 HSP 3 Score: 83.5741 bits (205), Expect = 5.121e-16 Identity = 63/203 (31.03%), Postives = 103/203 (50.74%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG--RKVLSRA--LRNRFIELHFNPIPNCELVEIL 594
R + E + ++ Y + ESV K+L LA V++ VL++G GKTSL+ YLA +TG +V + TD + +G Y ++ V++ G L QA GHWI+L++++ AP D++ L +L +N E+ I +K P F+ FAT+ G R + S A L + ++H + + EL E+L
Sbjct: 289 RSSSREQELALRSYVLVESVCKSLQTLAMAVASQNA--VLLEGPIGCGKTSLVEYLAAVTGRTKPPQLLKVQLGDQTDSKMLLGMY---RCTDVPGEFVWQPGTLTQAATMGHWILLEDIDYAPLDVVSVLIPLL-ENGELLIPGRGDCLKVAPGFQFFATRRLLSCGGNWYRPLNSHATLLDKYWTKIHLDNLDKRELNEVL 485 HSP 4 Score: 82.0333 bits (201), Expect = 1.500e-15 Identity = 57/181 (31.49%), Postives = 94/181 (51.93%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSSE-----TTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT---------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
TE + + + R + G+ PVL+ G+T GKT++ A L + V+ H + +++G V+Q ++ T+ + +G LVQAM+ + +LDE++LA +LE LN VL+ + + + E + + A FR+ AT NP G + G+K LS ALRNRF E+
Sbjct: 1366 TEGMRRLAMLVGRALEFGE--PVLLVGDTGCGKTTICQVFAALANQKLYSVSCHLHMETSDFLGGLRPVRQKPNDKEEIDTSRLFEWHDGPLVQAMKEDGFFLLDEISLADDSVLERLNSVLEVEKSLVLAEKGSPEDKDSEIELLTAGKKFRILATMNPGGDF-GKKELSPALRNRFTEI 1543 HSP 5 Score: 77.411 bits (189), Expect = 5.352e-14 Identity = 61/183 (33.33%), Postives = 96/183 (52.46%), Query Frame = 1
Query: 250 SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIES-QELDVVHCKRNYISLRDLFRWARRVYYEHNKC 765
++ QQ T +TL+F EG L QA++ G WI+LDE+NLA +ILE L+ +L+ + ++ +T+ ++ HP FRLFA NP G R L +RNRF EL+ + + E +++L + + KN + ++ L+ ES +L R + SLR L R R + N C
Sbjct: 801 NHAQQQMKMTENTLLFAFVEGTLAQAVKKGEWILLDEINLAAPEILECLSGLLEGSSGSLVLLDRGDTEPLVR-HPDFRLFACMNPATDVGKRN-LPPGIRNRFTELYVEELESKEDLQVLIVDYLKGLSVNKNTVQGIINFYTALRKESGTKLVDGTGHRPHYSLRTLCRALR--FAASNPC 979 HSP 6 Score: 56.9954 bits (136), Expect = 2.209e-7 Identity = 33/108 (30.56%), Postives = 60/108 (55.56%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFN 561
Q S + T + + +LVQA++ G W+++D +N +L+ LN +L+ + I E + TI +P FRLF + +P ++G +SRA+RNR +E++ +
Sbjct: 2211 TQLASGHSHGTFEWVDSMLVQALKSGDWLLMDNVNFCNPSVLDRLNALLEPGGVLTISERGMIDGSTPTITPNPNFRLFLSMDP--VHGD---ISRAMRNRGLEIYIS 2313
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q12019|MDN1_YEAST (Midasin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDN1 PE=1 SV=1) HSP 1 Score: 308.916 bits (790), Expect = 2.131e-93 Identity = 170/344 (49.42%), Postives = 221/344 (64.24%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARR--VYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y IT V KN+ +L R S GK PVL+QG TS GKTS+I YLA +TGH R+NNHE TD+QEY+G+YV ++ T L FKEGVLV+A+R G+WI+LDELNLAPTD+LEALNR+LDDNRE+FI ETQ + HP F LFATQNPPG+YGGRK+LSRA RNRF+ELHF+ IP EL IL E C+I + +K+VE R+L IE + K ++ +LRDLFRWA R V YE + GY+LL + R+ E V + L +V + V+L+ + +++ LE S L I ++ WT RR++VL ++ EPVLLVG
Sbjct: 1055 YIITPFVEKNMMNLVRATS-GKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTYV----TDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQEVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDELEIILRERCQIAPSYAKKIVEVYRQLSIERSASRLFEQKNSFATLRDLFRWALRDAVGYEQ------------LAASGYMLLAERCRTPQEKVTVKKTLEKVMK---VKLDMDQYYAS---------LEDKS-LEAIGSVTWTKGMRRLSVLVSSCLKNKEPVLLVG 1368 HSP 2 Score: 122.479 bits (306), Expect = 5.137e-29 Identity = 87/280 (31.07%), Postives = 145/280 (51.79%), Query Frame = 1
Query: 58 TESVSKNLT------DLARIVSAGKI-LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDE-SCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---------ISLRDLFRWARRVYYEHNKCD--RFFDWDRTMIEEGYLLLTGKARSK 840
+S S NLT +L R+V A ++ P+L++G VGKTSLIT LA +TG+ R+N E TD+ + G+ E + ++ + ++AM+ G W++LDE+NLA +LE LN LD E +I E + HP F +FA QNP GGRK L ++ NRF + + + + +L+ I I + KM++ M L+ D V CKR +LRD RW ++ +++ C+ FD+ ++++ + ++ K +++
Sbjct: 1714 AQSSSFNLTAPTTASNLVRVVRAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGA---DAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFSCHPNFLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLE------DQV-CKRKLWGNSGSPWEFNLRDTLRWL-KLLNQYSICEDVDVFDFVDIIVKQRFRTISDKNKAQ 1982 HSP 3 Score: 82.8037 bits (203), Expect = 9.805e-16 Identity = 75/266 (28.20%), Postives = 111/266 (41.73%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETT--------------------------------------DIQEYIGSYVQQNSSETTSTLVFK---------------EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE---TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL------HFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRW 732
PVL+ GET GKT++ LA+ G +N H+ T D++E + Y + ++ + + +G L+QAMR G++ +LDE++LA +LE LN VL+ R + + E + + + A F+ FAT NP G Y G+K LS ALRNRF E+ FN + N + L E K N K E K +L + ISLRD+ W
Sbjct: 1363 PVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLKELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATMNPGGDY-GKKELSPALRNRFTEIWVPSMEDFNDV-NMIVSSRLLEDLKDLANPIVKFSEWFGK------KLGGGNATSGVISLRDILAW 1620 HSP 4 Score: 79.7221 bits (195), Expect = 9.953e-15 Identity = 51/142 (35.92%), Postives = 78/142 (54.93%), Query Frame = 1
Query: 205 RVNNHET-------TDIQEYIGSYVQQNSS-ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVL--DDNREIFIVETQTT--IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEIL 594
R+N HE D + + + Q+SS E + F EG LV+ +R G W++LDE+NLA D LE+++ +L D+R I + E IKAHP FR+FA NP G R L +R+RF E++ + P ++ ++L
Sbjct: 773 RLNTHEKKLLLDKWADFNDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSILLSEKGDAEPIKAHPDFRIFACMNPATDVGKRD-LPMGIRSRFTEIYVHS-PERDITDLL 912 HSP 5 Score: 71.2478 bits (173), Expect = 5.607e-12 Identity = 42/124 (33.87%), Postives = 71/124 (57.26%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICH----RVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFAT 480
P+++ G+ GKT LI L+K G CH +++ E TD + IG+Y S + T ++ GVL A++ G W+++++++ APTD+L L +L + RE+ I T+KA F+L +T
Sbjct: 310 PIMLIGKAGSGKTFLINELSKYMG--CHDSIVKIHLGEQTDAKLLIGTYT---SGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLL-EKRELTIPSRGETVKAANGFQLIST 427 HSP 6 Score: 67.781 bits (164), Expect = 7.185e-11 Identity = 43/138 (31.16%), Postives = 70/138 (50.72%), Query Frame = 1
Query: 172 YLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPI 567
+ + L GH + + + E I + E + + +G+LV+A+ GHW+ILD NL +L+ LN +L+ + + I E +K HP FRLF T +P YG LSRA+RNR +E++ + +
Sbjct: 2160 FFSHLEGHPLLKTMSMNIEKMTEII-------TKEASVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMDPK--YGE---LSRAMRNRGVEIYIDEL 2285
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|O94248|MDN1_SCHPO (Midasin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mdn1 PE=1 SV=1) HSP 1 Score: 293.893 bits (751), Expect = 3.467e-88 Identity = 160/357 (44.82%), Postives = 226/357 (63.31%), Query Frame = 1
Query: 13 YNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARR--VYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
+ +EE + ++ IT V KNL ++AR S ++ P+L+QG TS GKTS+I Y+AK TGH R+NNHE TD+QEYIG+YV ++ +L F+EGVLV+A+R+G+WI+LDELNLAPTD+LEALNR+LDDNRE+FI ETQ +K HP F LFATQNPPG+Y GRK LSRA RNRF+E+HF+ IP EL IL + CKI + K+V+ R+L + Q + K ++ +LRDLFRWA R V Y+ + E GY+LL +AR + + V ++ +V + V+++ + ++ + I + + K L + WT R+ L R + EPVLLVG+
Sbjct: 865 FPVEEQEHYI----ITPFVQKNLLNIARACST-RMFPILIQGPTSSGKTSMIEYVAKKTGHKFVRINNHEHTDLQEYIGTYV----TDDNGSLSFREGVLVEALRNGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQVLVKPHPEFMLFATQNPPGVYAGRKHLSRAFRNRFLEIHFDDIPENELETILHKRCKIAPSYAAKIVQVFRELSLRRQTTRIFEQKNSFATLRDLFRWAFREAVGYQQ------------LAENGYMLLAERARDQKDKLAVQEVIEKVMK---VKIDTDGIYNLDSMEIFQDMSLKEGPLSKVV---WTRPMIRLFCLVWRCLLAKEPVLLVGD 1194 HSP 2 Score: 139.043 bits (349), Expect = 1.193e-34 Identity = 83/225 (36.89%), Postives = 128/225 (56.89%), Query Frame = 1
Query: 79 LTDLARIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC---KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---ISLRDLFRW 732
L + ++++ A ++L P+L++G VGKTSLIT LA+ TGH R+N + TD+ + GS V E +++ + AMR+GHW++LDELNLA +LE LN LD E +I E KAHP FR+FA QNP GGRK L R+ NRF ++ + +++EI +C ++ ++ K+++ M +LQ ++ E D+ +LRD RW
Sbjct: 1546 LMNASKVLRALQVLKPILLEGSPGVGKTSLITALARETGHQLVRINLSDQTDLMDLFGSDVPVEGGEG-GQFAWRDAPFLAAMRNGHWVLLDELNLASQSVLEGLNACLDHRNEAYIPELDKVFKAHPNFRVFAAQNPQHQGGGRKGLPRSFINRFSVVYVEALKEKDMIEI--AACNYHQVNEDWRLKIIKFMFRLQ-DNIEKDISFGSFGSPWEFNLRDTLRW 1766 HSP 3 Score: 80.8777 bits (198), Expect = 3.560e-15 Identity = 63/208 (30.29%), Postives = 93/208 (44.71%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAK------------------------------------LTGHICHRVNNHETTD-IQEYIGSYVQQNSSETTSTLV---------------FKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE---TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE 579
PVL+ G+T GKT++ LA+ L +C + N E+ D I + I + + +SSE L + +G LV AM+ G + +LDE++LA +LE LN VL+ +R + +VE ++ A F FAT NP G Y G+K LS ALRNRF E+ P+ + E
Sbjct: 1188 PVLLVGDTGCGKTTVCQILAECLHKELHIINAHQDTENGDIIGAQRPVRNRSAVNYSLHSQLCEKFNVQESLDSIDDLIEKFEKLSSSEKNDNLSNLIERQIIKYRSLFEWHDGALVTAMKQGDFFLLDEISLADDSVLERLNSVLELSRTLTLVEHSNAAVSLTAKDGFAFFATMNPGGDY-GKKELSPALRNRFTEIWVPPMVDTE 1394 HSP 4 Score: 72.0182 bits (175), Expect = 3.325e-12 Identity = 55/187 (29.41%), Postives = 84/187 (44.92%), Query Frame = 1
Query: 202 HRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT-------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELH----------------FNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHC 693
H H DI E + + S+ + +G L++A+ GHW +LD NL +L+ LN +L +++ + IV +T TIK HP FRLF T NP +YG LSRA+RNR +E+ P P V+ L + K + E M K++I+ + + H
Sbjct: 1985 HETLVHSLGDIHEKARKLLAEFSASPAGRFEWFDGYLLKAVEEGHWFVLDNANLCSPAVLDRLNSLL-EHKGVLIVNEKTTEDGHPKTIKPHPNFRLFLTVNP--VYGE---LSRAMRNRGVEIFLLKEALTEIDKKQMSLLEPAPISSAVDTLASNISYIK----YVFETMGKIEIDGNYMYIAHA 2161 HSP 5 Score: 70.0922 bits (170), Expect = 1.077e-11 Identity = 83/354 (23.45%), Postives = 155/354 (43.79%), Query Frame = 1
Query: 55 ITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNK----CDRFF---DWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSP------NGSNITKIILEKCSQLVNIANIEWTFDFRRIAVL--FIRSIQCDEPVLLV 1071
+T+S +NL + + + P+L+ G +GK LIT +A G R++ ++TD + IG+Y E ++ GVL QA+ G WI+ + AP+++L L +L + R++ I TI A F++FAT + K+L + L + ++L + P E+V L I + + + L + L R + LRD +++ +RV + ++K D + + +E + S+D +LV R +A + + + P + NI + L+K S + + + + F + +L +Q +EP+LLV
Sbjct: 134 LTKSTQENLNRITPYLVQKR--PILLAGPEGIGKKFLITQIAAKLGQQIIRIHLSDSTDPKMLIGTYTSPKPGE----FEWQPGVLTQAVITGKWILFTNIEHAPSEVLSVLLPLL-EKRQLVIPSRGETIYAKGSFQMFATSS-----MKTKILGQRLW-QILDLTYQPDECVEVVSTLYPVLSIICPTLYSVYKDIFDLFSQRSFLATSKIYRR-LCLRDFYKFIKRVAFLYHKFMIPSDHVVISQELQDAVFKEAIDMFGAFIPSRDGFDLVVRNVAIELNIPPEKALQLRYSIPVFQNLEHNINIGRCSLKKLSTIRSCSTNSYAFTSSSLGLLEQLAAGVQTNEPLLLV 473 HSP 6 Score: 67.0106 bits (162), Expect = 1.120e-10 Identity = 38/103 (36.89%), Postives = 59/103 (57.28%), Query Frame = 1
Query: 295 FKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ---TTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEIL 594
F EG LV+A+R GHW++LDE+NLA + LE + ++L ++ + T I H FRLF NP G R+ L + R+RF E++ + P+ L ++L
Sbjct: 627 FVEGALVKAVRSGHWVLLDEINLASLETLEPIGQLLSSYESGILLSERGDITPITPHKNFRLFGCMNPSTDVGKRE-LEPSFRSRFTEIYVHS-PDQNLDDLL 727
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|A0A1P8AUY4|MDN1_ARATH (Midasin OS=Arabidopsis thaliana OX=3702 GN=MDN1 PE=1 SV=1) HSP 1 Score: 293.123 bits (749), Expect = 5.878e-88 Identity = 166/354 (46.89%), Postives = 213/354 (60.17%), Query Frame = 1
Query: 28 TDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRT----MIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
+D+FV Y T+SV +L LA + K PVL+QG TS GKTSL+ YLA ++G+ R+NNHE TDIQEY+GSY+ ++++ LVF EG LV+A+R GHWI+LDELNLAP+D+LEALNR+LDDNRE+F+ E TI AHP F LFATQNPP LYGGRK+LSRA RNRF+E+H + IP EL EIL C I + KMVE M+ LQ Q K YI+ RDLFRWA RF +D T + EGY +L + R E +V +L FRVS + ++ + L + + N WT RR+ L RS + EPVLLVG+
Sbjct: 1044 SDKFVGSYVKTKSVIDHLNHLAHAIFI-KRYPVLLQGPTSSGKTSLVKYLAAISGNKFVRINNHEQTDIQEYLGSYM----TDSSGKLVFHEGALVKAVRGGHWIVLDELNLAPSDVLEALNRLLDDNRELFVPELSETISAHPNFMLFATQNPPTLYGGRKILSRAFRNRFVEIHVDEIPEDELSEILTTKCSIANSHASKMVEVMKDLQRNRQSSKAFAGKHGYITPRDLFRWAY----------RFRTYDGTSHEELAREGYYILAERLRDDTEKVVVQEVLERHFRVSLAK-----------DDLYNMELPRLDSIQN-RKFTWTQSMRRLFFLIDRSYKLREPVLLVGD 1370 HSP 2 Score: 133.265 bits (334), Expect = 1.069e-32 Identity = 71/184 (38.59%), Postives = 109/184 (59.24%), Query Frame = 1
Query: 40 VKGYK-ITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVE 588
+ G++ + + +N+ + R + K P+L++G VGKTSLI L K +GH R+N E TD+ + +GS + S E + +G+L+QA++ G W++LDELNLAP +LE LN +LD ++FI E T + P FR+FA QNP GGRK L ++ NRF +++ + ELVE
Sbjct: 1755 IGGFEFLAPTTHRNVLRVLRAMQLSK--PILLEGSPGVGKTSLILALGKYSGHKVVRINLSEQTDMMDLLGSDLPVESDEDMK-FAWSDGILLQALKEGSWVLLDELNLAPQSVLEGLNAILDHRAQVFIPELGCTFECPPTFRVFACQNPSTQGGGRKGLPKSFLNRFTKVYVD-----ELVE 1930 HSP 3 Score: 76.2554 bits (186), Expect = 1.273e-13 Identity = 59/201 (29.35%), Postives = 101/201 (50.25%), Query Frame = 1
Query: 25 ETDEFVKGYKITESVSKNLTDLARIVS----AGKILP----VLVQGETSVGKTSLITYLAKL----TGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT----IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE 579
ET +F+ G+ SK +T+ V + + P +++ G+ S + S+ + L G + + D E I + + + + V+++G LV+AMR G+ +++DE++LA +LE +N VL+ +R++ + E + AH F + AT NP G Y G+K LS ALRNRF E+ PI + E
Sbjct: 1400 ETSDFLGGFFPVRDRSKLITEYENQVKQLELSQALTPFGQDIVICGDISRAEVSIKSVEVALEKYKNGSVIGVAATPQDVDFLEKIRNNMVMLYQKWRAIFVWQDGPLVEAMRAGNIVLVDEISLADDSVLERMNSVLETDRKLSLAEKGGPVLEEVVAHEDFFVLATMNPGGDY-GKKELSPALRNRFTEIWVPPITDTE 1599 HSP 4 Score: 65.0846 bits (157), Expect = 5.216e-10 Identity = 36/90 (40.00%), Postives = 49/90 (54.44%), Query Frame = 1
Query: 289 LVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPM---FRLFATQNPPGLYGGRKVLSRALRNRFIE 549
F EG V A+R GHW++LDE+NLAP +IL L VL+ R + + + P FRLFA N P G++ L + R+RF E
Sbjct: 825 FTFVEGAFVTALREGHWVLLDEVNLAPPEILGRLIGVLEGVRGSLCLAERGDVMGIPRHLNFRLFACMN-PATDAGKRDLPFSFRSRFTE 913 HSP 5 Score: 64.6994 bits (156), Expect = 7.120e-10 Identity = 85/370 (22.97%), Postives = 159/370 (42.97%), Query Frame = 1
Query: 10 RYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGR-KVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVE-------AMR-KLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKI------ILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
R + + + F + ++I V K+ ++ VS + PVL+ G + GK++LI LA +G+ ++ + D + +G+YV ++ ++ G L QA+ +G W++L++++ AP+D+ L+ +L + F+ I+ F+LF+T + P + +L + + P L IL K++E A+R + + E + SLRDL +W RV+ + +D E + K+ V V+ I+A ++ V+ + D+ S I KI + E S + + T R+ RS++ +EPVLLVG
Sbjct: 320 RLDTWDVNSFSEPFEIHSRVKKSFEMVSLAVSQKR--PVLLYGPSGSGKSALIRKLADESGNHVVFIHMDDQLDGKTLVGTYV---CTDQPGEFRWQPGSLTQAIMNGFWVVLEDIDKAPSDVPLVLSSLLGGSCS-FLTSQGEEIRIAETFQLFSTISTPECSVSHIRDAGNSLSPLWRRIVVYPPDRESLQSILGARYPNLGPVAEKLIETFETINSALRPQFSSSTTENSATFSSPSRFSLRDLLKWCERVHGLPS-----YDGHAVYQEAADIFSASNMSVKNRV-AVSEIVASIWNVAVPESQDKPPIQ-EFSGILKIGRVSLPLGETASH--DRSRFVETRTSTRLLEKIARSVEYNEPVLLVG 674
BLAST of Midasin vs. UniProt/SwissProt
Match: sp|Q869L3|MDN1_DICDI (Midasin OS=Dictyostelium discoideum OX=44689 GN=mdn1 PE=3 SV=2) HSP 1 Score: 282.337 bits (721), Expect = 3.880e-84 Identity = 153/350 (43.71%), Postives = 224/350 (64.00%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHC-KRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFS----PNGSNITKIILEKCSQLVNIANIE---WTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y +T S+ NL+++AR++ + K P+L+QG TS GKTS++ YLA TGH R+NNHE TD+QEY+G Y+ S+ LVF+EG+LV+A+R G+W++LDELNLAP+++LEALNR+LDDNRE+FI ETQ ++ HP F LFATQNPPGLYGGRKVLSRA RNRF+ELH + IP EL EIL + C +P + +K+V M++LQ+ Q + V K I+ RDLFRWA R + + GY LL + R +E ++ +++ + + ++L+ E +S P + +++ E S+L ++E WT +R+ L + ++ EP+LLVG
Sbjct: 1107 YILTPSIKSNLSNIARVLVSRK-HPILLQGPTSSGKTSMVEYLAIRTGHRFIRINNHEHTDLQEYLGQYI----SDDKGKLVFQEGILVEAVRKGYWVVLDELNLAPSEVLEALNRLLDDNRELFIPETQEVVRPHPHFMLFATQNPPGLYGGRKVLSRAFRNRFLELHVDDIPEDELEEILCKRCALPPSYCKKLVLIMKELQLNRQGSNQVFAGKHGAITFRDLFRWAERQPSSYEELGI----------AGYQLLAERLRKDEEKLIIKQVIEKHLK---IKLDIESIYSCDDKPEFLKLLQLLSENDSELSKSNHLEKIVWTRSMKRLFSLVGKCLEHKEPILLVG 1438 HSP 2 Score: 129.413 bits (324), Expect = 2.434e-31 Identity = 84/229 (36.68%), Postives = 125/229 (54.59%), Query Frame = 1
Query: 64 SVSKNLTDLARIVSAGKILP--VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCK-IPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---ISLRDLFRW 732
+ SKN A+ V G LP +LV+G VGKTSLIT +A +G+ R+N E TDI + +GS + T +++GV ++A+R+G W++LDELNLA +LE LN LD E++I E T HP FR+FA QNP GGRK L ++ NRF ++ + + +L+ I IP +M+E ++ ES ++ ++ +LRD FRW
Sbjct: 1830 TTSKN----AQRVLRGMQLPRAILVEGSPGVGKTSLITAIANASGNQIVRINLSEQTDIMDLLGSDLPVEGG-TGGQFEWRDGVFLEALRNGSWVLLDELNLASQTVLEGLNSCLDHRSEVYIPELGKTFACHPNFRVFACQNPLHQGGGRKGLPKSFLNRFTQVFIDQLNQQDLLFISTAMYPTIPSKTIERMIEFNHQMFKESM-IEHKFARKGAPWEFNLRDTFRW 2052 HSP 3 Score: 90.8929 bits (224), Expect = 2.218e-18 Identity = 55/151 (36.42%), Postives = 93/151 (61.59%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI-CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG 507
T +VS+NL LA V GK P+L++G T GKT++I L+++TG+ R++ + TD + +G+Y+ +S+T ++ G L QA++ G WI++++++LAP ++L L +L +NR +FI I+A F+LFATQ L+G
Sbjct: 278 TNTVSQNLNSLAIAVGLGK--PILIEGVTGSGKTTMIEELSRITGNDNIIRIHLGDQTDSKVLLGTYI---TSDTPGEFKWQAGALTQAVQEGRWILIEDIDLAPIEVLSVLIPLL-ENRTLFIPGRGEVIEASNGFQLFATQT---LFGS 419 HSP 4 Score: 81.2629 bits (199), Expect = 2.974e-15 Identity = 52/151 (34.44%), Postives = 84/151 (55.63%), Query Frame = 1
Query: 301 EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT----IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE-LVEILDESCKIP--KNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRW 732
+G LV+AM+ G + ++DE++LA +LE LN VL+ +R + + E ++ H FR+ AT NP G + G+K LS A+RNRF E+ I + + L++I++E P K KG M+ + L + + + ISLRD+ W
Sbjct: 1568 DGPLVEAMKQGDYFLIDEISLAEDAVLERLNSVLEPSRLLVLAEKGGVEIEELRGHKEFRILATMNPGGDF-GKKELSPAMRNRFTEIWVPAISSHQDLLQIIEERFTSPQLKGKGSLMLNFIEYLLM-------IQKNKRVISLRDILSW 1710 HSP 5 Score: 71.2478 bits (173), Expect = 5.602e-12 Identity = 37/107 (34.58%), Postives = 60/107 (56.07%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT-TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPN 573
+Q S+ F EG LV +R G+W++LDE+NLA ++ LE+L+ + D + + ++ HP F++FA NPP G+K L +RNRF E + + + N
Sbjct: 847 IQFEKSKNNFAFSFVEGSLVNCIRKGYWVLLDEMNLATSETLESLSGLFDGGSITLTEKGEVEPVERHPNFKVFACMNPPTDI-GKKDLPPGIRNRFTEYYVDDLDN 952 HSP 6 Score: 53.5286 bits (127), Expect = 2.557e-6 Identity = 30/92 (32.61%), Postives = 53/92 (57.61%), Query Frame = 1
Query: 301 EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDN-----REIFIVETQT-TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF 558
+G+L++A+ G WI++D +N +L+ LN +L+ + E +V+ Q +K HP FR+F T + + +SRA+RNR IE++
Sbjct: 2365 DGLLIKALETGAWILIDNVNFCNPTVLDRLNPLLEQDGVLMLNERGMVDGQVKVVKPHPNFRIFLTMD-----DKKGEISRAMRNRGIEIYM 2451
BLAST of Midasin vs. TrEMBL
Match: A0A5K3FGN8 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1) HSP 1 Score: 368.237 bits (944), Expect = 2.067e-115 Identity = 183/357 (51.26%), Postives = 245/357 (68.63%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQ-------NSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKC----DRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNG----SNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y +TESV NL DLAR+VSA LPVL+QGETSVGKTSLITYLA G +C+R+NNHE TD+Q Y+G+Y ++ + +LVF+EG+ VQAM+ GHWIILDELNLAPT+ILEALNRVLD+NRE+FI ETQ +KAHP FR+FATQNPPGLY GRKVLSRALRNRF+ELHF+P+P EL IL+ +PK++ +K+VE M +LQ+ + D+ K +I+LRDLFRW R Y C ++ FDWD + E+GYLLL G+AR DE VA+++ VF+ +++++ F +G ++ + L ++ WT D RR+ VL +++ +EP+LLVG
Sbjct: 659 YVLTESVRANLKDLARVVSAAGSLPVLLQGETSVGKTSLITYLAGRVGQVCYRINNHEHTDLQTYLGAYTAASASRTAAENTTSAPSLVFQEGIFVQAMKKGHWIILDELNLAPTEILEALNRVLDENRELFITETQEMVKAHPHFRVFATQNPPGLYAGRKVLSRALRNRFVELHFDPLPRDELEVILEHRSALPKSRAKKLVEVMHQLQLLRRSSDIFQGKDGFITLRDLFRWGER--YRLATCNQTENQLFDWDTYLAEQGYLLLAGRARHPDEAKAVAQVIENVFK---RKVSEQTLFDLHGETSLASAEFLSLVDAPSPQGFGHVVWTRDMRRLLVLVGNALKYNEPILLVG 1010 HSP 2 Score: 71.633 bits (174), Expect = 9.435e-10 Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 1
Query: 271 SETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT---TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELH 555
S ++ + F EG LV+A+ G W++LDE+NLAP ++L+ L +LD + + +K HP F LFA NP G R L LR+RF E+H
Sbjct: 385 SASSLSFAFIEGALVRAVEEGDWVLLDEINLAPAELLDCLAGLLDSAHGSLTLVDRGDLEPVKRHPRFHLFAAMNPSTDVGKRD-LPMGLRDRFTEIH 481
BLAST of Midasin vs. TrEMBL
Match: A0A212EX73 (Midasin OS=Danaus plexippus plexippus OX=278856 GN=KGM_211783 PE=3 SV=1) HSP 1 Score: 379.022 bits (972), Expect = 2.697e-115 Identity = 194/352 (55.11%), Postives = 253/352 (71.88%), Query Frame = 1
Query: 46 GYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNK-----CDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFS--PNGSNITKIILEKC--SQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
GY +T +V KNL D+ARI+S G+ LPVL+QG+TSVGKTSLITY+AK +G+ C R+NNHE TD+QEYIGSY ++++T LVFKEGVLV+AMR G+WIILDELNLAP+D+LEALNRVLDDNRE+FI ETQ +KA P F LFATQNPPGLYGGRK+LSRA RNRF+ELHF+ IP EL IL + C IP ++M+E M +LQ+ + V K +I+LRDLFRW +R Y+H ++F+DWD+ + +EGYL+L GK R DE N++ ++ + + ++N E FS N S +TK ILE +QL ++I WTF+ RR+AVL ++ EPVLLVG
Sbjct: 1022 GYILTSTVRKNLRDIARIISLGR-LPVLLQGDTSVGKTSLITYIAKASGNYCVRINNHEHTDLQEYIGSY----ATDSTGNLVFKEGVLVEAMRKGYWIILDELNLAPSDVLEALNRVLDDNRELFIAETQEVVKADPNFMLFATQNPPGLYGGRKMLSRAFRNRFVELHFDEIPRAELEIILHQRCHIPPTYCKRMIEVMAELQVRRRGSAAVQGKDGFITLRDLFRWGQR--YKHASKEMLTTEKFYDWDQHIADEGYLILAGKVRQTDERNIIEEVIEKHIK---RKVNPENLFSLHDNTSPVTKSILEMILGNQLDAFSHIVWTFNMRRLAVLIAKAFIFSEPVLLVG 1363 HSP 2 Score: 123.25 bits (308), Expect = 7.713e-27 Identity = 75/206 (36.41%), Postives = 114/206 (55.34%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD-ESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRN--YISLRDLFRW 732
+L++G VGKTSL+T LAK +GH R+N + TDI + G+ + + FKEG + A+++G WI+LDELNLAP +LE LN LD E+FI E T K +LFA QNP G R+ L + NRF +++ + + +L+ I+ + +P++ K+VE K+ E E+ K ++LRD+ RW
Sbjct: 1707 ILLEGNPGVGKTSLVTALAKSSGHKVIRINLSDQTDITDLFGTDLPSEDG----SFTFKEGAFLNALQNGDWILLDELNLAPQAVLEGLNACLDHRGEVFIPELGKTFKVKKETKLFACQNPLKQGGARRGLPVSFLNRFTQVYIDTLTKEDLLYIVKTQYSNLPQDILHKIVEFNCKVAHEICEIQTWGHKGGPWEMNLRDIQRW 1908 HSP 3 Score: 92.4337 bits (228), Expect = 1.518e-16 Identity = 92/374 (24.60%), Postives = 167/374 (44.65%), Query Frame = 1
Query: 61 ESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGH---ICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGL-YGGRKVLSRA---LRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQEL-------------DVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWF---FSPNGS---NITKIIL------EKCSQLVNIANIEWTFDFRRIAVLFIRSIQC----DEPVLLVG 1074
+S NL LA V++G+ L ++ G GKT L+ +LA +TG +V + TD + +GS+ ++ V+ GVL +A+++GHW++L++++ A D+ L+ +L+ N + + + ++ P F+LF TQ YG +K +S + L+ +++ P+ EL EI+ +M+E + S + ++V IS RDL +W R E N D ++E+ + T S + +A+ + + V+ E++ + PN S N+ ++ EK + +N N + F F R + + I C +EPVLLVG
Sbjct: 281 KSTVNNLRSLALAVASGQALSLM--GPVGSGKTCLVEHLAAITGRKGIAFKKVQLGDQTDSRMLLGSH---QCTDVPGEFVWAPGVLTEAVQNGHWLLLEDIDCAALDVASTLSSLLERNT-LCVPGYRDSLPVTPGFQLFVTQRTMVTNYGFQKKMSNSSTLLQKHLTQINVEPLSRNELAEIIQTKYPNLSTICSRMIEVFMMFSVGSHDTSLHATSGSEKDCNNIVLRGSRLISTRDLMKWCARAVVEFNVSSP--DSAIKVLEDALDIFTCSISSPENRLELAKKVGTCLGI--VETKSEFYVKHYKPNVSLHPNVLEVGRAKVPRHEKRVESINFDNKQVVFSFTRQSACLLEKIACCVNSNEPVLLVG 644 HSP 4 Score: 81.6481 bits (200), Expect = 5.833e-13 Identity = 62/188 (32.98%), Postives = 101/188 (53.72%), Query Frame = 1
Query: 76 NLTDLARIVSAGKIL--PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY--VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ-------TTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE-LVEILDES 603
N+ LA +++ I PVL+ GET GKT++ LA L+ VN H T+ +++G V+Q +++ + +G L++AM +G + DE++LA +LE LN +L+ R++ I E I A F T NP G + G+K LS ALRNRF E+ + + + E L ++L++S
Sbjct: 1341 NMRRLAVLIAKAFIFSEPVLLVGETGCGKTTICQVLATLSKRNLLSVNCHMHTESSDFLGGLRPVRQYNNDG-RLFEWVDGPLIKAMENGDLFLADEISLADDSVLERLNSLLEPERQLVISERGMEDSSDIVVITAASSFHFIGTMNPGGDF-GKKELSPALRNRFTEIWCDRVSSKEDLQKVLEKS 1526 HSP 5 Score: 81.6481 bits (200), Expect = 7.125e-13 Identity = 53/147 (36.05%), Postives = 77/147 (52.38%), Query Frame = 1
Query: 157 TSLITYLAKLTGHICHRVNNHETTDIQEY-------IGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNRE-IFIVET--QTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPI 567
TSL+ + + R+ N + D+ + + QQ S + T F EG LV+AM+ GHW++LDE+NLA +ILE L +L+D E I ++E + I+ HP F LFA N P G+K L LRNRF E + +
Sbjct: 729 TSLLALMKQSHKAAVTRLTNEKNHDLLQVWVAFGHKLSKLDQQVKSASRLTFAFIEGSLVKAMQQGHWVLLDEINLASAEILECLAGLLEDKSESIDLLEKGDKVPIQRHPDFTLFACMN-PATDVGKKDLPVGLRNRFTEFFIDEL 874 HSP 6 Score: 72.4034 bits (176), Expect = 5.787e-10 Identity = 57/190 (30.00%), Postives = 90/190 (47.37%), Query Frame = 1
Query: 112 KILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY----------------------------VQQNSSETTSTLVFK-------------EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ--TTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
K L +LV G T+ GK+S++ LA LTG+ H V D+ + +G + ++QN+ S V K + +LV+AM G W++LD +NL + +L+ LN +L+ N + + E + IK HP FR+F T +P YG +SRA+RNR +E+
Sbjct: 2018 KWLSILV-GPTATGKSSIVQVLADLTGNDLHVVPVTSAMDVSDLLGFFEQVDYNRNIENLYEDIETATIQVARDVWIKQNTEGWKSNRVSKLSSVNAGGKFEWVDSLLVKAMVEGSWLLLDNVNLTSSAVLDRLNGLLEPNGVLSVPERGEISEIKPHPNFRVFFTMDPK--YGE---ISRAMRNRGVEI 2201
BLAST of Midasin vs. TrEMBL
Match: A0A5E4R6F4 (Uncharacterized protein OS=Leptidea sinapis OX=189913 GN=LSINAPIS_LOCUS15032 PE=4 SV=1) HSP 1 Score: 375.941 bits (964), Expect = 3.307e-114 Identity = 189/353 (53.54%), Postives = 255/353 (72.24%), Query Frame = 1
Query: 43 KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNK-----CDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFS--PNGSNITKIILEKC--SQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
+GY +T +V KNL D+ARI+S G+ LP+L+QG+TSVGKTSLITY+AK +G+ C R+NNHE TD+QEYIGSY +++ + +LVFKEGVLV+AMR G+WIILDELNLAP+D+LEALNRVLDDNRE+FI ETQ ++A P F LFATQNPPGLYGGRKV+SRA RNRF+ELHF+ IP EL IL + C IP +KM+E M +LQ+ + V K +I+LRDLFRW +R Y+H D+F+DWD+ + +EGYL+L GK R +E N++ ++ + + +++ E F+ N S +TK ILE +QL +++ WT++ RR+AVL ++ DEPVLLVG
Sbjct: 928 EGYILTTTVRKNLRDIARIISLGR-LPILLQGDTSVGKTSLITYIAKASGNYCVRINNHEHTDLQEYIGSY----ATDASGSLVFKEGVLVEAMRKGYWIILDELNLAPSDVLEALNRVLDDNRELFIPETQEVVRADPNFMLFATQNPPGLYGGRKVMSRAFRNRFVELHFDEIPRNELETILHQRCHIPPTYSKKMIEVMAELQVRRRGSAAVQGKDGFITLRDLFRWGQR--YKHASKETLTSDKFYDWDQHIADEGYLILAGKVRQVNERNIIEEVIEKHIK---RKIDPENLFTLHENTSAVTKSILETILNNQLPEFSHLVWTYNMRRLAVLIAKAFSFDEPVLLVG 1270 HSP 2 Score: 124.405 bits (311), Expect = 3.330e-27 Identity = 77/206 (37.38%), Postives = 117/206 (56.80%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD-ESCKIPKNKGRKMVEAMRKLQIESQELDV-VHCKRNY-ISLRDLFRW 732
+L++G VGKTSL+T LA +GH R+N + TDI + G+ + + VFKEGV + A+++G WI+LDELNLAP +LE LN LD EIFI E T K +LFA QNP G R+ L + NRF +++ + + +L I++ + ++PK+ ++VE K+ E E+ H + ++LRD+ RW
Sbjct: 1614 ILLEGNPGVGKTSLVTALAMSSGHRVVRINLSDQTDITDLFGTDLPSEDG----SFVFKEGVFLNALQNGDWILLDELNLAPQPVLEGLNACLDHRGEIFIPELGKTFKVKQQTKLFACQNPLKQGGARRGLPVSFLNRFTQVYIDTMTKDDLKYIVEAQYSQLPKDILHRIVEFNCKVSHEITEIQSWGHRGAPWEMNLRDVQRW 1815 HSP 3 Score: 93.2041 bits (230), Expect = 9.479e-17 Identity = 91/374 (24.33%), Postives = 161/374 (43.05%), Query Frame = 1
Query: 61 ESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGH---ICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGG--RKVLSRA--LRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCK-------------RNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWF---FSPNGS---------NITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQC----DEPVLLVG 1074
+S NL LA V++GK L ++ G GKTSL+ YLA +TG +V + TD + +GS+ ++ ++ GVL +A+++GHW++L++++ A D+ L+ +L+ N + + + + P F+LF TQ G RK S + L+ ++ P+ EL E++ KM+E + + + + IS RDL +W R N C D ++++ + + T S + +A+ + + V+ E++ + P+ S T LEK + ++ N E F F R + + I C +EPVLLVG
Sbjct: 206 KSTINNLRSLALAVASGKALSLM--GPVGSGKTSLVEYLAAVTGRKGIAFKKVQLGDQTDSRMLLGSH---QCTDIPGEFIWAPGVLTEAVQNGHWLLLEDIDSAALDVASTLSSLLERN-TLCVPGYRDVLPVTPGFQLFVTQRTVATNFGFQRKTSSSSALLQKHLTVINVEPLSRSELAEVVQTLYPSLTTITPKMIEVFMMFSVGCHDTSIQTTEAVDSENTVPFLRGSRLISTRDLMKWCARAVSGFNVCSA--DSALKVLQDSFDIFTCSVSSPENRLQLAKKVGTCLGI--VETKAEFYCKHYKPHVSLQPSSLVVGRATIPRLEKSLESIDFENKEVVFSFTRQSACLLERIACCVVSNEPVLLVG 569 HSP 4 Score: 85.8853 bits (211), Expect = 2.555e-14 Identity = 57/170 (33.53%), Postives = 92/170 (54.12%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLV-FKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT-------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPI-PNCELVEILDES 603
PVL+ GET GKT++ LA ++G VN H T+ ++IG + L + +G L+++M +G + DE++LA +LE +N +L+ R++ + E T IKA F T NP G + G+K LS ALRNRF E+ + I +L+++L++S
Sbjct: 1265 PVLLVGETGCGKTTICQVLAAMSGRSLLSVNCHMHTESSDFIGGLRPVRQYKNDGRLFEWVDGPLIKSMENGSLFLADEISLADDSVLERMNSLLEPERQLVLSERGTDESSDIVVIKAIKNFHFIGTMNPGGDF-GKKELSPALRNRFTEIWCDHISARSDLLKVLEKS 1433 HSP 5 Score: 80.8777 bits (198), Expect = 9.793e-13 Identity = 46/101 (45.54%), Postives = 60/101 (59.41%), Query Frame = 1
Query: 259 QQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDD---NREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
QQ +E+ T F EG LV+AM+ G+W++LDE+NLA +ILE L +L+D N E+ + IK HP F LFA N P G+K L LRNRF E
Sbjct: 695 QQVKAESRLTFAFIEGSLVKAMQEGYWVLLDEINLASAEILECLAGLLEDKNENVELLEKGDKVPIKRHPDFTLFACMN-PATDVGKKDLPVGLRNRFTEF 794
BLAST of Midasin vs. TrEMBL
Match: A0A2H1V7K6 (Midasin OS=Spodoptera frugiperda OX=7108 GN=SFRICE_001304 PE=3 SV=1) HSP 1 Score: 375.555 bits (963), Expect = 4.787e-114 Identity = 188/349 (53.87%), Postives = 247/349 (70.77%), Query Frame = 1
Query: 43 KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARR---VYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKC--SQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
+GY +T +V KNL D+ARI+S G+ LP+L+QG+TSVGKTSLITY+AK +G+ C R+NNHE TD+QEYIGSY ++++ LVFKEGVLV+AMR G+WIILDELNLAP+D+LEALNRVLDDNRE+FI ETQ +KA P F LFATQNPPGLYGGRK LSRA RNRF+ELHF+ IP EL IL + C +P +KM+ M LQI + V K +I+LRDLFRW +R E D+F+DWD+ + +EGYL+L GK R DE +++ ++ + + V ++ + P S +TK ILE +QL +++ WT++ RR+AVL +S DEPVLLVG
Sbjct: 1023 EGYILTPTVRKNLRDIARIISLGR-LPILLQGDTSVGKTSLITYIAKASGNYCVRINNHEHTDLQEYIGSY----ATDSNGKLVFKEGVLVEAMRKGYWIILDELNLAPSDVLEALNRVLDDNRELFIPETQQVVKADPNFMLFATQNPPGLYGGRKALSRAFRNRFVELHFDEIPKKELETILHQRCHVPPAYSKKMIAVMADLQIRRRGSAAVQGKDGFITLRDLFRWGQRYKCASKESLTDDKFYDWDQHIADEGYLILAGKVRQTDERSIIEEVIEKHIK-RKVNPDNLFTLHPTTSPVTKSILETILNNQLEEFSHVVWTYNMRRLAVLIAKSFSFDEPVLLVG 1365 HSP 2 Score: 129.028 bits (323), Expect = 8.126e-29 Identity = 78/210 (37.14%), Postives = 118/210 (56.19%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD-ESCKIPKNKGRKMVEAMRKLQIESQELDV-VHCKRNY-ISLRDLFRWARRV 744
+L++G VGKTSL+T LAK +GH R+N + TDI + G+ + + +FKEG + A+++G WI+LDELNLAP +LE LN LD E+FI E T K RLFA QNP G R+ L + NRF +++ + + +L+ I++ + IPK+ K+VE K+ E E+ H + ++LRD+ RW +
Sbjct: 1709 ILLEGNPGVGKTSLVTALAKSSGHKVVRINLSDQTDITDLFGTDLPTEDG----SFMFKEGAFLSALQNGDWILLDELNLAPQPVLEGLNACLDHRGEVFIPELGRTFKVKEQTRLFACQNPLKQGGARRGLPVSFLNRFTQVYIDTLTKEDLIYIVESQYASIPKDILHKIVEFNCKVAHEVTEIQSWGHRGAPWEMNLRDIQRWCEAI 1914 HSP 3 Score: 87.4261 bits (215), Expect = 8.015e-15 Identity = 88/378 (23.28%), Postives = 169/378 (44.71%), Query Frame = 1
Query: 61 ESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGH---ICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGL-YGGRKVLSRA---LRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIES-----QELDVVHCKRN----------YISLRDLFRWARR--VYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWF---FSPNGSNITKII---------LEKCSQLVNIANIEWTFDFRRIAVLFIRSIQC----DEPVLLVG 1074
+S NL LA V++G+ L ++ G GKTSL+ +LA +TG +V + TD + +GS+ ++ V++ GVL +A+++GHW++L++++ A D+ L+ +L+ N + + + ++ P F+LF TQ YG +K +S + L+ +++ P+ EL EI+ +M+E + S Q +VV + IS RDL +W R V ++ + + ++++ + T S + +++ + + V+ E++ + PN S + +EK + ++ + F F R + + I C +EPVLLVG
Sbjct: 282 KSTVNNLRSLALAVASGQALSLM--GPVGSGKTSLVEHLAAITGRKGVAFKKVQLGDQTDSRMLLGSH---QCTDIPGEFVWRPGVLTEAVQNGHWLLLEDIDCAALDVASTLSSLLERN-ALCVPGYRDSLPVTPGFQLFVTQRTLTTNYGFQKKISNSSTLLQKHLTQINVEPLSRSELAEIIQALYPALTTICSRMIEVFMMFSVGSHDTSLQTTEVVDSDKEKNLILMRGSRLISTRDLLKWCSRAVVGFDVSSPESAL----KVLQDALDIFTCSVASTEHRLELSKKIGLCLGI--VEAKSEFYCKHYKPNVSLTANFLEVGRSKVPRVEKSLESIDFDTKQTVFSFTRQSACLLERIACCVTSNEPVLLVG 647 HSP 4 Score: 84.3445 bits (207), Expect = 7.801e-14 Identity = 53/146 (36.30%), Postives = 80/146 (54.79%), Query Frame = 1
Query: 157 TSLITYLAKLTGHICHRVNNHETTDIQEYIGSYV------QQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNRE-IFIVET--QTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPI 567
T+L+ + + R+ ++ ++E++ V QQ + + T F EG LV+AM+ GHW++LDE+NLA +ILE L +L+D E I ++E + IK HP F LFA N P G+K L LRNRF EL + +
Sbjct: 732 TALLALMKQSQKAAVGRLTIEKSDKLKEWVALGVKLQKLEQQVKAASKLTFAFIEGSLVKAMQEGHWVLLDEINLASAEILECLAGLLEDKSETIDLLEKGDKVPIKRHPNFTLFACMN-PATDVGKKDLPAGLRNRFTELFIDEL 876 HSP 5 Score: 80.8777 bits (198), Expect = 1.176e-12 Identity = 58/178 (32.58%), Postives = 93/178 (52.25%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLV-FKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ-------TTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCE-LVEILDESCKIPKNKG 627
PVL+ GET GKT++ LA L VN H T+ +++G + L + +G L++AM G+ + DE++LA +LE +N +L+ R++ + E I A+ F T NP G + G+K LS ALRNRF E+ + + + E L+++L++S NKG
Sbjct: 1360 PVLLVGETGCGKTTICQVLAALKKCQLLTVNCHMHTESSDFLGGLRPVRQYQNDGRLFEWVDGPLIKAMETGNMFLADEISLADDSVLERMNSLLEPERQLVLAERGMENNSDIVVITANKNFHFIGTMNPGGDF-GKKELSPALRNRFTEIWCDSVTSREDLLKVLEKSV----NKG 1532 HSP 6 Score: 62.3882 bits (150), Expect = 1.183e-6 Identity = 37/102 (36.27%), Postives = 56/102 (54.90%), Query Frame = 1
Query: 259 QQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ--TTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF 558
Q +S F + +LV+ M G W++LD +NL +L+ LN +L+ N + I E + IK HP FR+F T +P YG +SRA+RNR +E+
Sbjct: 2177 QASSVNAGGKFEFVDSLLVKTMLEGSWLLLDNVNLTSAAVLDRLNGLLEPNGVLSIPERGEFSEIKPHPNFRVFFTMDPK--YGE---ISRAMRNRGVEISL 2273
BLAST of Midasin vs. TrEMBL
Match: A0A158QCU3 (Midasin OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS1958 PE=3 SV=1) HSP 1 Score: 373.629 bits (958), Expect = 2.295e-113 Identity = 192/370 (51.89%), Postives = 249/370 (67.30%), Query Frame = 1
Query: 19 IEETDEFV-KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTS---------TLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARR---VYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVN-----IANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
++E D+ V K Y +TE+V NL DLAR+VSA LPVL+QGETSVGKTSLITYLA G ICHR+NNHE TD+Q Y+GSY ++S + +LVF+EG+ VQAM+ GHWIILDELNLAPT+ILEALNRVLD+NRE+FI ETQ +KAHP FR+FATQNPPGLY GRKVLSRALRNRF+ELHF+ +P EL IL+ +P+++ KMVE M +LQ+ + D+ K +I+LRDLFRW R + K FDWD + E+GYLLL G+AR DEV VA ++ ++F+ ++ N + N N + E S + N +I WT D RR+ VL +++ +EP+LLVG
Sbjct: 833 LDEKDDVVRKSYVLTETVRGNLKDLARVVSAAGSLPVLLQGETSVGKTSLITYLAARVGQICHRINNHEHTDLQTYLGSYTAASASSVCTNSGDGSGVVSLVFQEGIFVQAMKQGHWIILDELNLAPTEILEALNRVLDENRELFITETQEMVKAHPHFRVFATQNPPGLYAGRKVLSRALRNRFVELHFDALPRAELEVILEHRSALPRSRASKMVEVMHQLQLIRRSSDIFQGKDGFITLRDLFRWGERYRLATFNQGKESHLFDWDTYLAEQGYLLLAGRARHSDEVKAVADVIQKIFKKKVLEEN---LYDRN-ENTSAAAAEFLSLIDNPLGGEFDHIVWTRDMRRLLVLVGNALKYNEPILLVG 1198 HSP 2 Score: 122.094 bits (305), Expect = 1.911e-26 Identity = 79/227 (34.80%), Postives = 115/227 (50.66%), Query Frame = 1
Query: 70 SKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPM-FRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDE-SCKIPKNKGRKMVEAMRKLQIESQELD---VVHCKRNYISLRDLFRWA 735
+ NL L R + K +L++G VGKTSL++ LA+ GH R+N E T+ + G + + T +++G L+QA+RHG+WI+LDE+NLA +LE LN LD I+I E T P RLFA QNP GGR+ L ++ NRF +++ P+ + + IL +P + MV LQ + D V +LRDL RW
Sbjct: 1591 ASNLCRLLRALQLPK-RALLLEGSPGVGKTSLVSALARAAGHRVVRINLSEATEASDLFGCDLPVEGA-GCGTFTWRDGPLLQALRHGYWILLDEMNLASQSVLECLNACLDHRGSIYIPELGVTFHVKPQSTRLFACQNPVEEGGGRRALPKSFLNRFTQVYLKPLTAADQIAILISIYNSLPVSHIEAMVRFNSMLQDAVERGDSFNAVAGTSWEFNLRDLCRWG 1815 HSP 3 Score: 87.0409 bits (214), Expect = 1.171e-14 Identity = 79/261 (30.27%), Postives = 121/261 (46.36%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYV-----QQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE----TQTT--IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCEL----VEILDESCKIPKNKGRKMVEA----MRKLQIE------SQELDVV--------HCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMI 798
P+L+ GET GKT++ A VN H+ T+ +++G Q T +F+ +G LV AM G +LDE++LA +LE LN +L+ R+I + E +Q + I A FRL AT NP G Y +K LS ALRNRF E+ + P P + +EI D + N R + A + K ++ ++V+ C+R + ++RDL W + + HN D+ R I
Sbjct: 1193 PILLVGETGCGKTTICQIFAAFRKQNLLCVNCHQYTEAADFLGGLRPVRTHQSGDPNITDDRLFEWVDGPLVVAMLQGEAFLLDEISLADDAVLERLNSLLEPERKICLAERYDDSQESEEITAAADFRLLATMNPGGDY-AKKELSPALRNRFTEI-WCPSPTFTVENSKIEITDWQAIVEHNLRRSDLLAGLTPLAKTMVDFCWWFAQARVEVMCGGMARKRRCRRPFPTIRDLLAW---LEFMHNLVSSKHDFSRASI 1448 HSP 4 Score: 75.8702 bits (185), Expect = 4.670e-11 Identity = 41/99 (41.41%), Postives = 59/99 (59.60%), Query Frame = 1
Query: 265 NSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNR-EIFIVETQT--TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
S+ T+ T F EG LV+A+ G W++LDE+NLAP ++L+ L +LD +R + +V+ ++ HP F LFA NP G R L LRNRF E+
Sbjct: 567 GSNSTSLTFAFIEGALVRALEEGDWVLLDEINLAPAELLDCLAGLLDSSRGSVTLVDRGDLEPVRRHPDFHLFAAMNPSTDVGKRD-LPMGLRNRFTEI 664 HSP 5 Score: 62.003 bits (149), Expect = 1.443e-6 Identity = 40/148 (27.03%), Postives = 67/148 (45.27%), Query Frame = 1
Query: 136 GETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQ-------TTIKAHPMFRLF--ATQNPPGLYGGRKVLSRALRNRFIEL 552
G GK SL+ A++ V + D E +G++ Q+ E + + LV+ +R GHW++L+ L +L+ LN +L+ ++ I E +K HP FRL N + +SRA+RNR +E+
Sbjct: 1931 GPRGAGKRSLVHLAAQIVRRHVSTVALSPSADTVELLGAFEQR---ENGGVFAWIDSPLVRGIREGHWVVLENAQLCSPSVLDRLNSLLEPGGDLLISERGLDANGDLVRLKPHPEFRLILVVDDNTAAVGSYSNNISRAMRNRGVEM 2075 HSP 6 Score: 61.6178 bits (148), Expect = 1.692e-6 Identity = 36/123 (29.27%), Postives = 60/123 (48.78%), Query Frame = 1
Query: 127 LVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVL----DDNREIFIVETQTTIKAHPMFRLFATQ 483
L+ G GKTS+I YLA++ R+ E TD + +G+Y N+ +++ G L M G W+IL++++ D+ L+ +L D + ++ T I HP FRL T+
Sbjct: 79 LICGPVGCGKTSVIDYLARIRNVKTFRMQISEQTDTRALLGAYCCSNA---PGVFIWRPGPLTHCMTAGKWLILEDVDKGSADLPILLSPILRNMKDSSSQVMHPNTGEPIPRHPDFRLILTR 198
BLAST of Midasin vs. Ensembl Cavefish
Match: mdn1 (midasin AAA ATPase 1 [Source:ZFIN;Acc:ZDB-GENE-041001-138]) HSP 1 Score: 339.732 bits (870), Expect = 4.022e-105 Identity = 181/345 (52.46%), Postives = 231/345 (66.96%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPN--GSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
Y +T SV NL DLAR+V+AG I PVL+QGETSVGKTSL+ +LA TG+ C R+NNHE TDIQEYIG Y SS+ LVFKEGVL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+FI ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M+ LQ + V K +I+LRDLFRWA R Y + D DW + + +GY+LL G+ R +E + IL + F+ + +N E FS + S + + ++ WT RR+AVL R+++ E VLLVG+
Sbjct: 473 YILTPSVRLNLRDLARVVAAG-IHPVLIQGETSVGKTSLVRWLAAATGNQCVRINNHEHTDIQEYIGCY----SSDEHGKLVFKEGVLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFIAETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSEELETILHQRCSLPPSYCTKLVKVMQNLQSLRRGSSVFAGKHGFITLRDLFRWAER-YRLEEQTDTSRDWLQHLANDGYMLLAGRVRRPEEALTIQNILEKHFKRT---VNPEALFSEDQVTSQFSPFVASIAGVPEEFGHVVWTEGMRRLAVLVGRALRFGESVLLVGD 808 HSP 2 Score: 107.842 bits (268), Expect = 4.460e-25 Identity = 72/225 (32.00%), Postives = 118/225 (52.44%), Query Frame = 1
Query: 94 RIVSAGKIL-PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHP-MFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
R++ A K+ P+L++G VGKTSL+ LAK +G+ R+N E TD+ + G+ + + +++G L+ A++ GHWI+LDELNLA +LE LN D EI+I E ++F QNP GGRK L ++ NRF ++ + + E ++ + S I N +++ M +Q ++ + V +R + +LRDLFRW +
Sbjct: 1168 RVLRALKLQRPILLEGSPGVGKTSLVAALAKASGNHLIRINLSEQTDVTDLFGTDLPVEGGKG-GEFAWRDGPLLAALKAGHWIVLDELNLASQSVLEGLNACFDHRAEIYIPELSMRFHVQQEKTKIFGCQNPFTQGGGRKGLPKSFLNRFTQVFVDQL-TSEDMQFIGNS--IFPNINEQIISKM--VQFSNKLVHEVTVERKWGQKGGPWEFNLRDLFRWCQ 1386 HSP 3 Score: 81.6481 bits (200), Expect = 2.260e-16 Identity = 67/227 (29.52%), Postives = 109/227 (48.02%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY-------VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT-------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPN--CELVEILDESCKIP------KNKGRKMVEAMRKL--QIESQELDVVHCKRNYISLRDLFRW 732
VL+ G+T GKT++ A L G VN H + +++G +E +++G LV AMR ++DE++LA +LE LN VL+ + + + E + I A FRL AT NP G + G+K LS ALRNRF E+ + P N +L++I+ + + ++G+ + E M + +QE +R +S+RD+ W
Sbjct: 803 VLLVGDTGCGKTTICQLFAALAGQKFFSVNCHLHMETSDFLGGLRPVRHAGTGHGDAEDGRLFEWRDGPLVLAMRQEGVFLMDEISLADDSVLERLNSVLETEKSLVLAEKGSGDDDDVELIIAGKKFRLVATMNPGGDF-GKKELSPALRNRFTEI-WCPQTNSRSDLMKIIQHNLRSGLSLDQRDHQGKDIAELMLDFIDWLTNQEFG----RRCILSVRDILSW 1023 HSP 4 Score: 68.1662 bits (165), Expect = 5.496e-12 Identity = 51/145 (35.17%), Postives = 71/145 (48.97%), Query Frame = 1
Query: 175 LAKLTGHICHRVNNHETTD------IQEYIG------SYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV----ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELH 555
L KL H+C E D +QE S Q+ S LVF EG L QA++ G WI+LDE+NLA + LE L+ +L+ + ++ +T+ ++ H FRLFA NP G R L +RNRF EL+
Sbjct: 182 LLKLMDHVCKSALTKELQDKPNAALLQEQWEALALKLSQTQEQIRARESALVFAFVEGTLAQAVKKGEWILLDEINLAAPETLECLSGLLEGSAGSLVLLDRGDTEPLVR-HSDFRLFACMNPATDVGKRN-LPLGIRNRFTELY 324 HSP 5 Score: 61.2326 bits (147), Expect = 9.966e-10 Identity = 32/92 (34.78%), Postives = 51/92 (55.43%), Query Frame = 1
Query: 301 EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF 558
+G LVQA++ G W+++D +N +L+ LN +L+ + I E T +I HP FRLF T +P +SRA+RNR +E++
Sbjct: 1657 DGTLVQALQAGDWLLMDNVNFCSPSVLDRLNALLEPGGSLTINERGVIDGTTPSITPHPNFRLFLTMDP-----AHGEISRAMRNRGVEIYI 1743 HSP 6 Score: 51.9878 bits (123), Expect = 7.335e-7 Identity = 29/69 (42.03%), Postives = 39/69 (56.52%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSY 255
+ T S L LA V+ G+ PVL+ GET GKTS + YLA LTGH VN ++ +D + +G Y
Sbjct: 68 FAATRPSSVLLEQLAVCVTKGE--PVLLVGETGTGKTSTVQYLAGLTGHKLRVVNMNQQSDTADLLGGY 134
BLAST of Midasin vs. Ensembl Cavefish
Match: vwa8 (von Willebrand factor A domain containing 8 [Source:NCBI gene;Acc:561317]) HSP 1 Score: 51.2174 bits (121), Expect = 1.518e-6 Identity = 46/189 (24.34%), Postives = 79/189 (41.80%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDI-------LEALNRVLDDNREIFIVETQT-TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHF--NPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQ 660
+L+ G VGK ++ L + H T +Q + S +V+++ LV+A++ GH +++DE + APT++ +E+ +L D R I + I HP FR+ N PG +L + F H NP P E + +P +K+V A +L+
Sbjct: 713 LLLVGNQGVGKNKIVDRFLHLLNRPREYLQLHRDTTVQT-----LTLQPSVRDGIIVYEDSPLVKAVKMGHILVIDEADKAPTNVTCILKTLVESGEMILADGRRIVSASGRPNAIPMHPDFRMIVLANRPGFPFLGNDFFGSLGDIF-SCHAVDNPKPQAEFAMLKQYGPDVPDAVLQKLVAAFGELR 895
BLAST of Midasin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007003.1 (pep scaffold:Pmarinus_7.0:GL481838:489617:494557:1 gene:ENSPMAG00000006330.1 transcript:ENSPMAT00000007003.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 64.3142 bits (155), Expect = 1.289e-11 Identity = 36/124 (29.03%), Postives = 63/124 (50.81%), Query Frame = 1
Query: 127 LVQGETSVGKTSLITYLAKLTGHICHR----VNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQN 486
L++G GKT+L+ LA LTG V + TD + +G Y ++ ++ G L QA+ G WI+L++++ AP D++ L +L+ + + ++A P F+LFAT+
Sbjct: 322 LIEGPVGCGKTALVERLAALTGRTAPPGLLTVQLGDQTDSRTLLGMY---RCTDVPGEFTWRAGALTQAVSRGDWILLEDIDYAPLDVISVLIPLLESG-VLSVPGRGDCVRAAPGFQLFATRR 441
BLAST of Midasin vs. Ensembl Yeast
Match: MDN1 (Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus [Source:SGD;Acc:S000004096]) HSP 1 Score: 308.916 bits (790), Expect = 3.069e-95 Identity = 170/344 (49.42%), Postives = 221/344 (64.24%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARR--VYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y IT V KN+ +L R S GK PVL+QG TS GKTS+I YLA +TGH R+NNHE TD+QEY+G+YV ++ T L FKEGVLV+A+R G+WI+LDELNLAPTD+LEALNR+LDDNRE+FI ETQ + HP F LFATQNPPG+YGGRK+LSRA RNRF+ELHF+ IP EL IL E C+I + +K+VE R+L IE + K ++ +LRDLFRWA R V YE + GY+LL + R+ E V + L +V + V+L+ + +++ LE S L I ++ WT RR++VL ++ EPVLLVG
Sbjct: 1055 YIITPFVEKNMMNLVRATS-GKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTYV----TDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQEVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDELEIILRERCQIAPSYAKKIVEVYRQLSIERSASRLFEQKNSFATLRDLFRWALRDAVGYEQ------------LAASGYMLLAERCRTPQEKVTVKKTLEKVMK---VKLDMDQYYAS---------LEDKS-LEAIGSVTWTKGMRRLSVLVSSCLKNKEPVLLVG 1368 HSP 2 Score: 122.479 bits (306), Expect = 7.398e-31 Identity = 87/280 (31.07%), Postives = 145/280 (51.79%), Query Frame = 1
Query: 58 TESVSKNLT------DLARIVSAGKI-LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDE-SCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY---------ISLRDLFRWARRVYYEHNKCD--RFFDWDRTMIEEGYLLLTGKARSK 840
+S S NLT +L R+V A ++ P+L++G VGKTSLIT LA +TG+ R+N E TD+ + G+ E + ++ + ++AM+ G W++LDE+NLA +LE LN LD E +I E + HP F +FA QNP GGRK L ++ NRF + + + + +L+ I I + KM++ M L+ D V CKR +LRD RW ++ +++ C+ FD+ ++++ + ++ K +++
Sbjct: 1714 AQSSSFNLTAPTTASNLVRVVRAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGA---DAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFSCHPNFLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLE------DQV-CKRKLWGNSGSPWEFNLRDTLRWL-KLLNQYSICEDVDVFDFVDIIVKQRFRTISDKNKAQ 1982 HSP 3 Score: 82.8037 bits (203), Expect = 1.412e-17 Identity = 75/266 (28.20%), Postives = 111/266 (41.73%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETT--------------------------------------DIQEYIGSYVQQNSSETTSTLVFK---------------EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE---TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL------HFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRW 732
PVL+ GET GKT++ LA+ G +N H+ T D++E + Y + ++ + + +G L+QAMR G++ +LDE++LA +LE LN VL+ R + + E + + + A F+ FAT NP G Y G+K LS ALRNRF E+ FN + N + L E K N K E K +L + ISLRD+ W
Sbjct: 1363 PVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLKELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATMNPGGDY-GKKELSPALRNRFTEIWVPSMEDFNDV-NMIVSSRLLEDLKDLANPIVKFSEWFGK------KLGGGNATSGVISLRDILAW 1620 HSP 4 Score: 79.7221 bits (195), Expect = 1.433e-16 Identity = 51/142 (35.92%), Postives = 78/142 (54.93%), Query Frame = 1
Query: 205 RVNNHET-------TDIQEYIGSYVQQNSS-ETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVL--DDNREIFIVETQTT--IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEIL 594
R+N HE D + + + Q+SS E + F EG LV+ +R G W++LDE+NLA D LE+++ +L D+R I + E IKAHP FR+FA NP G R L +R+RF E++ + P ++ ++L
Sbjct: 773 RLNTHEKKLLLDKWADFNDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSILLSEKGDAEPIKAHPDFRIFACMNPATDVGKRD-LPMGIRSRFTEIYVHS-PERDITDLL 912 HSP 5 Score: 71.2478 bits (173), Expect = 8.074e-14 Identity = 42/124 (33.87%), Postives = 71/124 (57.26%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICH----RVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFAT 480
P+++ G+ GKT LI L+K G CH +++ E TD + IG+Y S + T ++ GVL A++ G W+++++++ APTD+L L +L + RE+ I T+KA F+L +T
Sbjct: 310 PIMLIGKAGSGKTFLINELSKYMG--CHDSIVKIHLGEQTDAKLLIGTYT---SGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLL-EKRELTIPSRGETVKAANGFQLIST 427 HSP 6 Score: 67.781 bits (164), Expect = 1.035e-12 Identity = 43/138 (31.16%), Postives = 70/138 (50.72%), Query Frame = 1
Query: 172 YLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQT------TIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPI 567
+ + L GH + + + E I + E + + +G+LV+A+ GHW+ILD NL +L+ LN +L+ + + I E +K HP FRLF T +P YG LSRA+RNR +E++ + +
Sbjct: 2160 FFSHLEGHPLLKTMSMNIEKMTEII-------TKEASVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMDPK--YGE---LSRAMRNRGVEIYIDEL 2285
BLAST of Midasin vs. Ensembl Medaka
Match: mdn1 (midasin AAA ATPase 1 [Source:NCBI gene;Acc:101161112]) HSP 1 Score: 312.768 bits (800), Expect = 1.192e-95 Identity = 173/342 (50.58%), Postives = 223/342 (65.20%), Query Frame = 1
Query: 49 YKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVG 1074
Y +T SV NL DLAR+VSA PVL+QGETSVGKTSLI +LA TG+ C R+NNHE TDIQEYIG Y SS++ LVFKE VL+ AMR G+WIILDELNLAPTD+LEALNR+LDDNRE+F+ ETQ +KAHP F LFATQNPPGLYGGRKVLSRA RNRF+ELHF+ +P+ EL IL + C +P + K+V+ M LQ + V K +I+LRDLFRWA R Y + + DW + + ++ ++LL G+ R +E + L + F+ +++ E F + S ++ WT RR+AVL R+++ E VLLVG
Sbjct: 1020 YILTASVKLNLRDLARVVSATH--PVLIQGETSVGKTSLIRFLAAATGNQCVRINNHEHTDIQEYIGCY----SSDSRGKLVFKE-VLIDAMRKGYWIILDELNLAPTDVLEALNRLLDDNRELFVAETQEVVKAHPRFMLFATQNPPGLYGGRKVLSRAFRNRFVELHFDELPSGELESILHQRCSLPPSYCSKLVKVMLDLQSLRRGSSVFAGKHGFITLRDLFRWADR-YRLEEQTEASQDWLQHLADD-FMLLAGRVRKPEEEVTILSTLEKHFK---RKVDPEALFCQK--QVASQFPSVGSAPEGFGHVVWTGGMRRLAVLVGRALRFGESVLLVG 1347 HSP 2 Score: 114.775 bits (286), Expect = 2.429e-27 Identity = 76/218 (34.86%), Postives = 118/218 (54.13%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDE-LNLAPTDILEALNRVLDDNREIFIVETQTTIKA-HPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESC--KIPKNKGRKMVEAMRKLQIESQELDVVHCKRNY--------ISLRDLFRWAR 738
PVL++G VGKTSL+ LAK +G+ R+N E TD+ + G+ + + +++G L+ A++ GHW++LDE LNLA +LE LN D EIFI E + + H ++F QNP GGRK L ++ NRF ++ + + + E +E + +S I K KMVE KLQ+ + V +R++ +LRD+FRW +
Sbjct: 1702 PVLLEGSPGVGKTSLVAALAKASGNHLVRINLSEQTDVTDLFGTDLPVEGGK-GGEFAWRDGPLLAALKAGHWVVLDEQLNLASQSVLEGLNACFDHRAEIFIPELGLSFQVQHEKTKIFGCQNPFTQGGGRKGLPKSFLNRFTQVFVDQL-SSEDMEFIADSIFPNIDKEIRVKMVEFSNKLQLVQE----VCVQRHWGQRGSPWEFNLRDMFRWCQ 1913 HSP 3 Score: 80.8777 bits (198), Expect = 4.331e-16 Identity = 68/220 (30.91%), Postives = 114/220 (51.82%), Query Frame = 1
Query: 124 VLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGS-----YVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT-------IKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPN--CELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVH---CKRNYISLRDLFRW 732
VL+ GET GKT++ A L G + VN H + +++G + Q ++ + + +G LV A++ G ++DE++LA +LE LN VL+ + + + E + IKA FRL AT NP G + G+K LS ALRNRF E+ + P N +LV+I++ + + G + + +L ++ E + H +R +S+RD+ W
Sbjct: 1343 VLLVGET-CGKTTICQMFAALAGQTLYSVNCHLHMETSDFLGGLRPVRHSHQTDADDSRLFQWHDGPLVIALKEGGVFLMDEISLADDSVLERLN-VLETEKCLVLAEKGSGDDDDVELIKADQAFRLVATMNPGGDF-GKKELSPALRNRFTEI-WCPQSNRRSDLVQIINHNL----HSGLSLKGDIAELMLDFIEW-LTHQDFGRRCILSVRDVLSW 1553 HSP 4 Score: 73.1738 bits (178), Expect = 1.386e-13 Identity = 45/152 (29.61%), Postives = 81/152 (53.29%), Query Frame = 1
Query: 43 KGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHI----CHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQN 486
K + S ++L LA V++ K PVL++G GKT+L+ +LA TGH +V + TD + +G Y ++ V++ G L QA+ G WI+L++++ AP D++ L ++ +++++ I I P F+ FAT++
Sbjct: 284 KNLVLVGSTCRDLRRLALAVASQK--PVLLEGPIGCGKTALVEFLAAATGHWKASEIMKVQLGDQTDSKMLLGMY---RCTDVPGKFVWQPGTLTQAVAKGQWILLEDIDHAPLDVVSVLLPLM-ESKKLLIPGRDDCIDVAPGFQFFATRS 429 HSP 5 Score: 71.633 bits (174), Expect = 3.897e-13 Identity = 68/231 (29.44%), Postives = 100/231 (43.29%), Query Frame = 1
Query: 175 LAKLTGHICHRV---NNHETTDIQEY---IGSYVQQN-----SSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIV--ETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILD----ESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLL 816
L KL H+C E +QE + S + Q + ET F EG L QA++ GHWI+LDE+NLA + LE L+ +L+ + ++ T + HP FRLF NP G R L LRN E + + N + IL + PKN ++ + + + L R + SLR L R + Y N C R++ E+G+ L
Sbjct: 734 LLKLMDHVCKSALTKGLQEKASLQEQWEALSSRLSQTQQQIRTCETAMVFAFVEGTLAQAVKKGHWILLDEINLAAAETLECLSGLLEGSAGSLVLLDRGDTPLVRHPDFRLFGCMNPATDVGKRN-LPLGLRN-LTEFYVEELENEGDLRILVSDYLKGLNPPKNVVTGVIFYLNVRKEANSSLVDGTGHRPHYSLRTLCRALK--YVAGNPCQSV---QRSLYEQGFCL 957 HSP 6 Score: 53.5286 bits (127), Expect = 2.966e-7 Identity = 36/105 (34.29%), Postives = 56/105 (53.33%), Query Frame = 1
Query: 256 VQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE------TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
QQ T + +G+LVQA++ G W+++D N + +L+ LN +L+ N + + E I HP F LF T +P ++G LSRA+RNR +EL
Sbjct: 2159 AQQEDGWTNGGFEWLDGMLVQALQTGDWLLMDNANFCAS-VLDRLNALLEPNGSLTVSERGVIDGKTPKISPHPDF-LFLTMDP--VHG---ELSRAMRNRGVEL 2256
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033713.1 (SMESG000033713.1) HSP 1 Score: 745.732 bits (1924), Expect = 0.000e+0 Identity = 358/359 (99.72%), Postives = 358/359 (99.72%), Query Frame = 1
Query: 1 SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVL IRSIQCDEPVLLVGN
Sbjct: 783 SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLLIRSIQCDEPVLLVGN 1141 HSP 2 Score: 119.783 bits (299), Expect = 3.247e-29 Identity = 90/290 (31.03%), Postives = 138/290 (47.59%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHP-MFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPK-------------NKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWAR-RVYYEH-NKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEV 879
+++ N+ L R + K P+L++G VGKTSL+T LAKL G R+N E TD + G ++ N + L +++G L+QA++ G WI+LDE+NLA +LE LN LD EIFI E P RLFA QNP GRK L ++ NRF +++ + + + + IL+ C P R+ V M+K + + +LRDL RW V ++H N FF LL + R+K++ + ++ E+
Sbjct: 1472 SKTTGSNVYRLFRALQVSK-KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFLVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWE--------FNLRDLCRWCDLMVKHQHGNHEPSFF---------VSLLFIKRFRTKEDQDACGKVYNEL 1740 HSP 3 Score: 82.8037 bits (203), Expect = 7.036e-17 Identity = 55/159 (34.59%), Postives = 86/159 (54.09%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE--------TQTTIK-----AHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
PVL+ G T GKT++ A L +N H T+ +++G + T +F+ +G LV AM+ G ++DE++LA +LE LN VL+ +R++ +VE ++T I+ A F++ AT NP G + G+K LS ALRNRF E+
Sbjct: 1135 PVLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLGGLKPAVNKTEDETRLFQWVDGPLVVAMKQGKMFLIDEISLADDSVLERLNSVLEKDRKLTLVENGDANLLTSETEIQSVQICADKNFQVVATMNPGGDF-GKKELSPALRNRFTEI 1292 HSP 4 Score: 70.0922 bits (170), Expect = 9.090e-13 Identity = 41/131 (31.30%), Postives = 66/131 (50.38%), Query Frame = 1
Query: 118 LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT-------IKAHPMFRLFATQNP 489
L VLV G GKT+++ L+ +TG+ ++ + D + +GS+ QQ + + LVQA+R G W+ILD +N +L+ LN +L+ N + + E + IK H FRLF +P
Sbjct: 1809 LTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGSVYGDRLEEIKPHKNFRLFLILDP 1935 HSP 5 Score: 63.5438 bits (153), Expect = 1.059e-10 Identity = 36/104 (34.62%), Postives = 63/104 (60.58%), Query Frame = 1
Query: 271 SETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE---TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPN 573
S+ + + EG LV+A+R+G W++LDE+NLAP ++L+ L+ +L+ + + +T + H FRLFA NP G++ L + LRNRF E++ + + +
Sbjct: 536 SKCSLMFAYIEGALVKAIRNGDWVLLDEINLAPPEMLQGLSGLLESSSGSVTLTEKGDETPVPRHQDFRLFACMNPATDV-GKQNLPQGLRNRFTEVYLHELTD 638 HSP 6 Score: 63.1586 bits (152), Expect = 1.433e-10 Identity = 58/229 (25.33%), Postives = 102/229 (44.54%), Query Frame = 1
Query: 112 KILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIF------IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEI----LDESCKIPKNKGRKMVEAMRK-------LQIESQELDVVH-CKRNYISLRDLFRWARRV 744
K +P+L+ G GK+ LI Y+AKL G RV + +D + +G Y ++ ++ G L+ AM+ G WIIL+++ +++L L +L+ I I AH FR+ TQ+ G + + F + N + C+ + + D I K+ ++ + K LQ+ ++ DV + C+ N S RDLF++ R+
Sbjct: 53 KQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLGGYC---CTDIPGEFEWRSGPLITAMKRGQWIILEDILSGQSNLLGMLRPLLEAESLGLKPKISNITSLNEEIIAHEDFRIILTQSLLEFDEGYREMK--FGGTFSPYNANCLNKCQTILLDKLHTDSIESIVKSNYESLIPFLPKMLGDQGLLQLVVKQFDVKYQCRIN--SPRDLFKFCHRI 274
BLAST of Midasin vs. Planmine SMEST
Match: SMESG000033713.1 (SMESG000033713.1) HSP 1 Score: 745.732 bits (1924), Expect = 0.000e+0 Identity = 358/359 (99.72%), Postives = 358/359 (99.72%), Query Frame = 1
Query: 1 SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLFIRSIQCDEPVLLVGN 1077
SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVL IRSIQCDEPVLLVGN
Sbjct: 761 SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPKNKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWARRVYYEHNKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEVFRVSDVQLNDEWFFSPNGSNITKIILEKCSQLVNIANIEWTFDFRRIAVLLIRSIQCDEPVLLVGN 1119 HSP 2 Score: 119.783 bits (299), Expect = 3.365e-29 Identity = 90/290 (31.03%), Postives = 138/290 (47.59%), Query Frame = 1
Query: 58 TESVSKNLTDLARIVSAGKILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTTIKAHP-MFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEILDESCKIPK-------------NKGRKMVEAMRKLQIESQELDVVHCKRNYISLRDLFRWAR-RVYYEH-NKCDRFFDWDRTMIEEGYLLLTGKARSKDEVNLVARILAEV 879
+++ N+ L R + K P+L++G VGKTSL+T LAKL G R+N E TD + G ++ N + L +++G L+QA++ G WI+LDE+NLA +LE LN LD EIFI E P RLFA QNP GRK L ++ NRF +++ + + + + IL+ C P R+ V M+K + + +LRDL RW V ++H N FF LL + R+K++ + ++ E+
Sbjct: 1450 SKTTGSNVYRLFRALQVSK-KPLLLEGSPGVGKTSLVTQLAKLVGFKVVRINLSEQTDANDLFGCFLP-NENGKAGELSWRDGPLLQALKAGDWILLDEMNLASQSVLECLNACLDHRGEIFIPEINQKFLVEPGKTRLFACQNPVSEGSGRKGLPKSFVNRFTQVYMSSLSDSDYRNILN--CLYPAIARETIGFIVEFVQNLREHVVVMKKFGTKGSPWE--------FNLRDLCRWCDLMVKHQHGNHEPSFF---------VSLLFIKRFRTKEDQDACGKVYNEL 1718 HSP 3 Score: 82.8037 bits (203), Expect = 7.555e-17 Identity = 55/159 (34.59%), Postives = 86/159 (54.09%), Query Frame = 1
Query: 121 PVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFK--EGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE--------TQTTIK-----AHPMFRLFATQNPPGLYGGRKVLSRALRNRFIEL 552
PVL+ G T GKT++ A L +N H T+ +++G + T +F+ +G LV AM+ G ++DE++LA +LE LN VL+ +R++ +VE ++T I+ A F++ AT NP G + G+K LS ALRNRF E+
Sbjct: 1113 PVLLVGNTGCGKTTICQLAAMLNQQELLTINCHMHTEASDFLGGLKPAVNKTEDETRLFQWVDGPLVVAMKQGKMFLIDEISLADDSVLERLNSVLEKDRKLTLVENGDANLLTSETEIQSVQICADKNFQVVATMNPGGDF-GKKELSPALRNRFTEI 1270 HSP 4 Score: 70.0922 bits (170), Expect = 9.408e-13 Identity = 41/131 (31.30%), Postives = 66/131 (50.38%), Query Frame = 1
Query: 118 LPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVETQTT-------IKAHPMFRLFATQNP 489
L VLV G GKT+++ L+ +TG+ ++ + D + +GS+ QQ + + LVQA+R G W+ILD +N +L+ LN +L+ N + + E + IK H FRLF +P
Sbjct: 1787 LTVLV-GPAGTGKTTIVKLLSDITGNHLKSMSMNSAMDTTDLLGSFEQQKEG---GKFKWVDSTLVQALRQGDWLILDTVNFCSASVLDRLNGLLEPNGSLLLTERGSVYGDRLEEIKPHKNFRLFLILDP 1913 HSP 5 Score: 63.5438 bits (153), Expect = 1.156e-10 Identity = 36/104 (34.62%), Postives = 63/104 (60.58%), Query Frame = 1
Query: 271 SETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIFIVE---TQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPN 573
S+ + + EG LV+A+R+G W++LDE+NLAP ++L+ L+ +L+ + + +T + H FRLFA NP G++ L + LRNRF E++ + + +
Sbjct: 514 SKCSLMFAYIEGALVKAIRNGDWVLLDEINLAPPEMLQGLSGLLESSSGSVTLTEKGDETPVPRHQDFRLFACMNPATDV-GKQNLPQGLRNRFTEVYLHELTD 616 HSP 6 Score: 63.1586 bits (152), Expect = 1.470e-10 Identity = 58/229 (25.33%), Postives = 102/229 (44.54%), Query Frame = 1
Query: 112 KILPVLVQGETSVGKTSLITYLAKLTGHICHRVNNHETTDIQEYIGSYVQQNSSETTSTLVFKEGVLVQAMRHGHWIILDELNLAPTDILEALNRVLDDNREIF------IVETQTTIKAHPMFRLFATQNPPGLYGGRKVLSRALRNRFIELHFNPIPNCELVEI----LDESCKIPKNKGRKMVEAMRK-------LQIESQELDVVH-CKRNYISLRDLFRWARRV 744
K +P+L+ G GK+ LI Y+AKL G RV + +D + +G Y ++ ++ G L+ AM+ G WIIL+++ +++L L +L+ I I AH FR+ TQ+ G + + F + N + C+ + + D I K+ ++ + K LQ+ ++ DV + C+ N S RDLF++ R+
Sbjct: 31 KQMPILLTGPVGSGKSVLIDYMAKLMGKELCRVQISDESDSKTLLGGYC---CTDIPGEFEWRSGPLITAMKRGQWIILEDILSGQSNLLGMLRPLLEAESLGLKPKISNITSLNEEIIAHEDFRIILTQSLLEFDEGYREMK--FGGTFSPYNANCLNKCQTILLDKLHTDSIESIVKSNYESLIPFLPKMLGDQGLLQLVVKQFDVKYQCRIN--SPRDLFKFCHRI 252 The following BLAST results are available for this feature:
BLAST of Midasin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Midasin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of Midasin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of Midasin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of Midasin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Midasin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of Midasin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Midasin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Midasin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of Midasin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Midasin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Midasin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Midasin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Midasin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030711 ID=SMED30030711|Name=Midasin|organism=Schmidtea mediterranea sexual|type=transcript|length=1079bpback to top protein sequence of SMED30030711-orf-1 >SMED30030711-orf-1 ID=SMED30030711-orf-1|Name=SMED30030711-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=359bp SEYRYNIEETDEFVKGYKITESVSKNLTDLARIVSAGKILPVLVQGETSVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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