CDV3 homolog
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030691 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of CDV3 homolog vs. Ensembl Human
Match: CDV3 (CDV3 homolog [Source:HGNC Symbol;Acc:HGNC:26928]) HSP 1 Score: 54.6842 bits (130), Expect = 4.133e-8 Identity = 41/130 (31.54%), Postives = 64/130 (49.23%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TK++DEW++ EVD S LR+ M E K E++ E D DN ++KS+ PW ++A PV+++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKDEDEWKELEQKEVDYSGLRVQAMQISSE----KEEDDNEKRQDPGDNWEEGGGGGGGMEKSSGPWNKTAPVQAPPAPVIVTETPEPAMTSGVYRPPGARLTTT--RKTPQGPPEIYSDTQFPSLQSTA 202
BLAST of CDV3 homolog vs. Ensembl Human
Match: CDV3 (CDV3 homolog [Source:HGNC Symbol;Acc:HGNC:26928]) HSP 1 Score: 53.9138 bits (128), Expect = 1.284e-7 Identity = 41/130 (31.54%), Postives = 64/130 (49.23%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TK++DEW++ EVD S LR+ M E K E++ E D DN ++KS+ PW ++A PV+++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKDEDEWKELEQKEVDYSGLRVQAMQISSE----KEEDDNEKRQDPGDNWEEGGGGGGGMEKSSGPWNKTAPVQAPPAPVIVTETPEPAMTSGVYRPPGARLTTT--RKTPQGPPEIYSDTQFPSLQSTA 202
BLAST of CDV3 homolog vs. Ensembl Human
Match: CDV3 (CDV3 homolog [Source:HGNC Symbol;Acc:HGNC:26928]) HSP 1 Score: 52.373 bits (124), Expect = 1.969e-7 Identity = 39/129 (30.23%), Postives = 63/129 (48.84%), Query Frame = 3 Query: 276 KEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 +++DEW++ EVD S LR+ M E K E++ E D DN ++KS+ PW ++A PV+++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 34 RDEDEWKELEQKEVDYSGLRVQAMQISSE----KEEDDNEKRQDPGDNWEEGGGGGGGMEKSSGPWNKTAPVQAPPAPVIVTETPEPAMTSGVYRPPGARLTTT--RKTPQGPPEIYSDTQFPSLQSTA 156
BLAST of CDV3 homolog vs. Ensembl Xenopus
Match: cdv3 (CDV3 homolog [Source:NCBI gene;Acc:448377]) HSP 1 Score: 51.9878 bits (123), Expect = 5.284e-7 Identity = 44/140 (31.43%), Postives = 67/140 (47.86%), Query Frame = 3 Query: 225 EKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLS-EPSQPQK-YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 EKS +S+ P + KEDDEW++ EVD S LRI + E+ + E E +D D DKS+ PW +A + V+ + EP Y PP R + ++P+ P I ++++FPSL+ A Sbjct: 64 EKSGKSENPDQ---LQEKEDDEWKEFEQKEVDYSGLRIQSLQISNEKDDDEYEKKEEQGADWEDIGGC--GTDKSSGPWNKTAQAQAPISAVIEAPEPVHTGGVYRPPAAR--ASVATRKPQGPPEIFSDTQFPSLQATA 196
BLAST of CDV3 homolog vs. UniProt/SwissProt
Match: sp|Q9UKY7|CDV3_HUMAN (Protein CDV3 homolog OS=Homo sapiens OX=9606 GN=CDV3 PE=1 SV=1) HSP 1 Score: 53.9138 bits (128), Expect = 6.164e-7 Identity = 41/130 (31.54%), Postives = 64/130 (49.23%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TK++DEW++ EVD S LR+ M E K E++ E D DN ++KS+ PW ++A PV+++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKDEDEWKELEQKEVDYSGLRVQAMQISSE----KEEDDNEKRQDPGDNWEEGGGGGGGMEKSSGPWNKTAPVQAPPAPVIVTETPEPAMTSGVYRPPGARLTTT--RKTPQGPPEIYSDTQFPSLQSTA 202
BLAST of CDV3 homolog vs. UniProt/SwissProt
Match: sp|Q5ZLH9|CDV3_CHICK (Protein CDV3 homolog OS=Gallus gallus OX=9031 GN=CDV3 PE=2 SV=1) HSP 1 Score: 52.7582 bits (125), Expect = 1.016e-6 Identity = 39/129 (30.23%), Postives = 67/129 (51.94%), Query Frame = 3 Query: 270 KTKEDDEW-EDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN-DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 K+KE+D+W E E+D S LR+ M ++ K ++ +E + SDN + VD+S+ PW A V +++EP +P + Y PP R+ + P + + P I ++++FPSL+ A Sbjct: 74 KSKEEDDWKEFEQKEEIDYSGLRVQSM-----QISEKEDDESEKREEPSDNWEETSGAVDRSSGPWNKSAPAPAPVVETIVTEPPEPVQTGGVYRPPGAREGGR-PRRAQQGPPEIYSDTQFPSLQSTA 196
BLAST of CDV3 homolog vs. UniProt/SwissProt
Match: sp|Q6DIS2|CDV3_XENTR (Protein CDV3 homolog OS=Xenopus tropicalis OX=8364 GN=cdv3 PE=2 SV=1) HSP 1 Score: 51.9878 bits (123), Expect = 2.799e-6 Identity = 44/140 (31.43%), Postives = 67/140 (47.86%), Query Frame = 3 Query: 225 EKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLS-EPSQPQK-YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 EKS +S+ P + KEDDEW++ EVD S LRI + E+ + E E +D D DKS+ PW +A + V+ + EP Y PP R + ++P+ P I ++++FPSL+ A Sbjct: 64 EKSGKSENPDQ---LQEKEDDEWKEFEQKEVDYSGLRIQSLQISNEKDDDEYEKKEEQGADWEDIGGC--GTDKSSGPWNKTAQAQAPISAVIEAPEPVHTGGVYRPPAAR--ASVATRKPQGPPEIFSDTQFPSLQATA 196
BLAST of CDV3 homolog vs. TrEMBL
Match: A0A096NDE8 (CDV3 homolog OS=Papio anubis OX=9555 GN=CDV3 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 1.444e-6 Identity = 41/130 (31.54%), Postives = 66/130 (50.77%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TK++DEW++ EVD S LR+ M ++ K E++TE D DN ++KS+ PW ++A PV+++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKDEDEWKELEQKEVDYSGLRVQAM-----QISEKEEDDTEKRQDPGDNWEEGGGGGGGMEKSSGPWNKTALVQAPPAPVIVTETPEPAMTSGVYRPPGARLTTT--RKTPQGPPEIYSDTQFPSLQSTA 201
BLAST of CDV3 homolog vs. TrEMBL
Match: A0A2I3M821 (CDV3 homolog OS=Papio anubis OX=9555 GN=CDV3 PE=4 SV=1) HSP 1 Score: 59.3066 bits (142), Expect = 1.609e-6 Identity = 41/130 (31.54%), Postives = 66/130 (50.77%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TK++DEW++ EVD S LR+ M ++ K E++TE D DN ++KS+ PW ++A PV+++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKDEDEWKELEQKEVDYSGLRVQAM-----QISEKEEDDTEKRQDPGDNWEEGGGGGGGMEKSSGPWNKTALVQAPPAPVIVTETPEPAMTSGVYRPPGARLTTT--RKTPQGPPEIYSDTQFPSLQSTA 201
BLAST of CDV3 homolog vs. TrEMBL
Match: A0A452GBL9 (CDV3 homolog OS=Capra hircus OX=9925 GN=CDV3 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 2.070e-6 Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TKE+DEW++ EVD S LR+ M ++ K E+ E D SDN V+KS+ PW ++A PVV++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKEEDEWKEFEQKEVDYSGLRVQAM-----QISEKEEDEVEKREDPSDNWEEGGGGGGGVEKSSGPWNKTAPVQAPPAPVVVTETPEPTMTSGVYRPPGARLTTT--RKAPQGPPEIYSDTQFPSLQSTA 201
BLAST of CDV3 homolog vs. TrEMBL
Match: A0A4W2DZ67 (CDV3 homolog OS=Bos indicus x Bos taurus OX=30522 GN=CDV3 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 2.070e-6 Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TKE+DEW++ EVD S LR+ M ++ K E+ E D SDN V+KS+ PW ++A PVV++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 79 TKEEDEWKEFEQKEVDYSGLRVQAM-----QISEKEEDEVEKREDPSDNWEEGGGGGGGVEKSSGPWNKTAPVQAPPAPVVVTETPEPTMTSGVYRPPGARLTTT--RKAPQGPPEIYSDTQFPSLQSTA 201
BLAST of CDV3 homolog vs. TrEMBL
Match: A0A2F0B776 (Protein CDV3 (Fragment) OS=Eschrichtius robustus OX=9764 GN=ESR_42358 PE=4 SV=1) HSP 1 Score: 58.151 bits (139), Expect = 2.103e-6 Identity = 42/130 (32.31%), Postives = 66/130 (50.77%), Query Frame = 3 Query: 273 TKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDN----DNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQK----YVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVA 638 TK++DEW++ EVD S+LR+ M ++ K E+ E D DN V+KS+ PW +++A PVV++E +P Y PP R + K P+ P I ++++FPSL+ A Sbjct: 35 TKDEDEWKEFEQKEVDHSSLRVQAM-----QISEKEEDENEKREDPGDNWEEGGGGGGGVEKSSGPWNKTASVQAPPAPVVVTETPEPTMTSGVYRPPGARLTTT--RKAPQGPPEIYSDTQFPSLQSTA 157
BLAST of CDV3 homolog vs. Planmine SMEST
Match: SMESG000008267.1 (SMESG000008267.1) HSP 1 Score: 532.332 bits (1370), Expect = 0.000e+0 Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 3 Query: 75 MQIKMEANDLESFFLXXXXXXXXXXANIINPALLIEQTREAEIKKAKKVNEKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQKYVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVAINDTEDGTWCRVIKNGHTQPSEPVTKKYIPPAKKELVQTDSQPTIEKAKTEKIGPSKYVPPHLRNKQSTNPSENRFASLKDESMN 893 MQIKMEANDLESFFLSKDKKKKSKKANIINPALLIEQTREAEIKKAKKVNEKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQKYVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVAINDTEDGTWCRVIKNGHTQPSEPVTKKYIPPAKKELVQTDSQPTIEKAKTEKIGPSKYVPPHLRNKQSTNPSENRFASLKDESMN Sbjct: 1 MQIKMEANDLESFFLSKDKKKKSKKANIINPALLIEQTREAEIKKAKKVNEKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQKYVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVAINDTEDGTWCRVIKNGHTQPSEPVTKKYIPPAKKELVQTDSQPTIEKAKTEKIGPSKYVPPHLRNKQSTNPSENRFASLKDESMN 273
BLAST of CDV3 homolog vs. Planmine SMEST
Match: SMESG000008267.1 (SMESG000008267.1) HSP 1 Score: 523.472 bits (1347), Expect = 0.000e+0 Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 3 Query: 87 MEANDLESFFLXXXXXXXXXXANIINPALLIEQTREAEIKKAKKVNEKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQKYVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVAINDTEDGTWCRVIKNGHTQPSEPVTKKYIPPAKKELVQTDSQPTIEKAKTEKIGPSKYVPPHLRNKQSTNPSENRFASLKDESMN 893 MEANDLESFFLSKDKKKKSKKANIINPALLIEQTREAEIKKAKKVNEKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQKYVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVAINDTEDGTWCRVIKNGHTQPSEPVTKKYIPPAKKELVQTDSQPTIEKAKTEKIGPSKYVPPHLRNKQSTNPSENRFASLKDESMN Sbjct: 1 MEANDLESFFLSKDKKKKSKKANIINPALLIEQTREAEIKKAKKVNEKSHQSQEPKSELLRKTKEDDEWEDTADVEVDMSNLRIGMMPQKVEEVKPKIENNTECASDESDNDNIFKKVDKSAHPWGNKITIKAEVEPVVLSEPSQPQKYVPPNMRDISKAPMKQPKKAPNITNNSEFPSLREVAINDTEDGTWCRVIKNGHTQPSEPVTKKYIPPAKKELVQTDSQPTIEKAKTEKIGPSKYVPPHLRNKQSTNPSENRFASLKDESMN 269 The following BLAST results are available for this feature:
BLAST of CDV3 homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of CDV3 homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of CDV3 homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of CDV3 homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 3
BLAST of CDV3 homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of CDV3 homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of CDV3 homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of CDV3 homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030691 ID=SMED30030691|Name=CDV3 homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=1163bpback to top protein sequence of SMED30030691-orf-1 >SMED30030691-orf-1 ID=SMED30030691-orf-1|Name=SMED30030691-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=274bp MQIKMEANDLESFFLSKDKKKKSKKANIINPALLIEQTREAEIKKAKKVNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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