Thyroglobulin

Overview
NameThyroglobulin
Smed IDSMED30030687
Length (bp)566
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Thyroglobulin (SMED30030687) t-SNE clustered cells

Violin plots show distribution of expression levels for Thyroglobulin (SMED30030687) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030687

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30030687h1SMcG0020834 SmedASXL_003167SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30030687h1SMcG0020834 SmedASXL_003167SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30030687h1SMcG0020834 dd_Smed_v6_26987_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Thyroglobulin vs. Ensembl Human
Match: TG (thyroglobulin [Source:HGNC Symbol;Acc:HGNC:11764])

HSP 1 Score: 56.6102 bits (135), Expect = 5.304e-9
Identity = 30/103 (29.13%), Postives = 52/103 (50.49%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKK-------SHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPG 481
             +P C + G+Y  VQC +G+CWC N  G    + P ++ +       + C+  RA ++    S    +  F   C ++G++  VQC  + C+C D +G  +PG
Sbjct:  616 FVPSCTTEGSYEDVQCFSGECWCVNSWG---KELPGSRVRGGQPRCPTDCEKQRARMQSLMGSQPAGSTLFVPACTSEGHFLPVQCFNSECYCVDAEGQAIPG 715

HSP 2 Score: 48.9062 bits (115), Expect = 1.845e-6
Identity = 30/111 (27.03%), Postives = 51/111 (45.95%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQNG--KCWCSNRDGITIHDYPDAKKK----SHCKCARASIEFKQNSDGPITAFF--CKTDGNYGDVQC--KQTTCFCTDEDGVRMPGKATVDYEKR 508
            +P+C  +G++  VQCQN    CWC   +G  +       +     S C+  +  I      +   T++   C+  G+Y  VQC  +Q  C+C D +G+ + G   +   KR
Sbjct:   49 VPQCAEDGSFQTVQCQNDGRSCWCVGANGSEVLGSRQPGRPVACLSFCQLQKQQILLSGYINSTDTSYLPQCQDSGDYAPVQCDVQQVQCWCVDAEGMEVYGTRQLGRPKR 159
[Close]
BLAST of Thyroglobulin vs. Ensembl Human
Match: CD74 (CD74 molecule [Source:HGNC Symbol;Acc:HGNC:1697])

HSP 1 Score: 46.595 bits (109), Expect = 8.409e-6
Identity = 34/110 (30.91%), Postives = 47/110 (42.73%), Query Frame = 2 
Query:   56 LLVEATR-GCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDG 376
            LL E +R   E      PPKV            TKC +           +  P+CD NGNY P+QC    G CWC   +G  +   P+ + + H  C+  S+E +  S G
Sbjct:  184 LLFEMSRHSLEQKPTDAPPKVL-----------TKCQEEVSHIPAVHPGSFRPKCDENGNYLPLQCYGSIGYCWCVFPNGTEV---PNTRSRGHHNCSE-SLELEDPSSG 278
[Close]
BLAST of Thyroglobulin vs. Ensembl Human
Match: CD74 (CD74 molecule [Source:HGNC Symbol;Acc:HGNC:1697])

HSP 1 Score: 46.595 bits (109), Expect = 9.139e-6
Identity = 34/110 (30.91%), Postives = 47/110 (42.73%), Query Frame = 2 
Query:   56 LLVEATR-GCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDG 376
            LL E +R   E      PPKV            TKC +           +  P+CD NGNY P+QC    G CWC   +G  +   P+ + + H  C+  S+E +  S G
Sbjct:  189 LLFEMSRHSLEQKPTDAPPKVL-----------TKCQEEVSHIPAVHPGSFRPKCDENGNYLPLQCYGSIGYCWCVFPNGTEV---PNTRSRGHHNCSE-SLELEDPSSG 283
[Close]
BLAST of Thyroglobulin vs. Ensembl Zebrafish
Match: tg (thyroglobulin [Source:ZFIN;Acc:ZDB-GENE-030519-1])

HSP 1 Score: 57.7658 bits (138), Expect = 1.157e-9
Identity = 32/107 (29.91%), Postives = 49/107 (45.79%), Query Frame = 2 
Query:  176 KAIKSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHC-----KCARASIEFKQNSDGPITAFF--CKTDGNYGDVQCKQTTCFCTDEDGVRM 475
            K + S +   +P+C  +G Y  VQCQ  +CWC +  G+ +        +  C     K  + +I  K +S      F   C+TDG Y   QC   +CFC D  G ++
Sbjct:  596 KPMSSSSAPYLPQCTEDGLYQDVQCQGSECWCVDSRGLEVPGSRTTGSRPRCPSQCEKERQMAIAVKASSSAGSEVFIPKCETDGAYVARQCLGKSCFCVDRSGTKL 702
[Close]
BLAST of Thyroglobulin vs. Ensembl Zebrafish
Match: nid2b (nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-040724-143])

HSP 1 Score: 51.9878 bits (123), Expect = 9.963e-8
Identity = 44/159 (27.67%), Postives = 62/159 (38.99%), Query Frame = 2 
Query:   86 DSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMA-----DTLIPRCDSNGNYAPVQC--QNGKCWCSNRDGI----------TIH---DYPDA-KKKSHCKCARASIEFKQNSDGPITAF----FCKTDGNYGDVQCKQTT--CFCTDEDGVRMPG 481
            D F C+PP           P  T C Q      +SM+     +  +P+CD  G Y P+QC    G CWC +  G           T H   D P A   +    C R  +    + +G  ++      C   GN+  VQC   +  C+C D  GV + G
Sbjct:  706 DGFLCQPPPK------RPKPSLTACQQHRNSLQESMSMYPALEAFVPKCDDKGQYKPLQCLGSTGHCWCVDSRGQERVGTRTMPGTAHANCDQPAALVPRVETVCERWRLALMSHYNGQPSSQDYMPLCDAHGNFQPVQCYGNSAFCWCVDRHGVEIIG 858
[Close]
BLAST of Thyroglobulin vs. Ensembl Zebrafish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 49.6766 bits (117), Expect = 3.274e-7
Identity = 43/145 (29.66%), Postives = 58/145 (40.00%), Query Frame = 2 
Query:  131 CYANPLTTKCFQIY-----GKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIH-------------DYPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            CY +P  T+C Q       G          IP+CD  G Y P+QC    G CWC +  G                 D P  + K+HC+  R S++  +N   P+   F   C  +G Y   QC  +T  C+C D  G    G  T
Sbjct:   46 CYGHP-KTRCEQHRDSLQSGDGGVPAVGAFIPQCDEEGQYRPLQCHGSTGHCWCVDNRGQERAGTRTPPGSPPRNCDEP-VRPKTHCEQHRDSLQ-SENGGVPVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRT 187
[Close]
BLAST of Thyroglobulin vs. Ensembl Zebrafish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 48.521 bits (114), Expect = 1.869e-6
Identity = 36/116 (31.03%), Postives = 49/116 (42.24%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIH-------------DYPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
             IP+CD  G Y P+QC    G CWC +  G                 D P  + K+HC+  R S++  +N   P+   F   C  +G Y   QC  +T  C+C D  G    G  T
Sbjct:  859 FIPQCDEEGQYRPLQCHGSTGHCWCVDNRGQERAGTRTPPGSPPRNCDEP-VRPKTHCEQHRDSLQ-SENGGVPVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRT 972
[Close]
BLAST of Thyroglobulin vs. Ensembl Zebrafish
Match: nid2b (nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-040724-143])

HSP 1 Score: 48.1358 bits (113), Expect = 1.959e-6
Identity = 44/159 (27.67%), Postives = 61/159 (38.36%), Query Frame = 2 
Query:   86 DSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMA-----DTLIPRCDSNGNYAPVQC--QNGKCWCSNRDGI----------TIH---DYPDAK-KKSHCKCARASIEFKQNSDGPITAF----FCKTDGNYGDVQC--KQTTCFCTDEDGVRMPG 481
            D F C+PP           P  T C Q      +SM+     +  +P+CD  G Y P+QC    G CWC +  G           T H   D P A   +    C R  +    + +G  ++      C   GN+  VQC      C+C D  GV + G
Sbjct:   57 DGFLCQPPPK------RPKPSLTACQQHRNSLQESMSMYPALEAFVPKCDDKGQYKPLQCLGSTGHCWCVDSRGQERVGTRTMPGTAHANCDQPAALVPRVETVCERWRLALMSHYNGQPSSQDYMPLCDAHGNFQPVQCYGNSAFCWCVDRHGVEIIG 209
[Close]
BLAST of Thyroglobulin vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 53.5286 bits (127), Expect = 4.194e-8
Identity = 39/139 (28.06%), Postives = 56/139 (40.29%), Query Frame = 2 
Query:  143 PLTTKCF----QIYGKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDG--ITIHDYPDAKKKSHC----------KCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            PL T C     Q++ +  +      +P CD  GNY P+QC    G CWC ++ G  IT    P  +   +C           C     +       P    F   C  +GNY  +QC  +T  C+C D+ G  + G  T
Sbjct:  923 PLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRT 1061
[Close]
BLAST of Thyroglobulin vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 52.7582 bits (125), Expect = 8.553e-8
Identity = 41/143 (28.67%), Postives = 60/143 (41.96%), Query Frame = 2 
Query:  143 PLTTKCF----QIYGKAIKSMADTLIPRCDSNGNYAPVQCQ--NGKCWCSNRDG--ITIHDYPDAKK------------KSHCKCARASIEFKQNSDGPITAFF-----CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            PL T C     Q++ +  +      +P CD  GNY P+QC    G CWC ++ G  IT    P  +             K+ C   R  +  + +  GP  A       C  +GNY  +QC  +T  C+C D+ G  + G  T
Sbjct:  923 PLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRT 1065
[Close]
BLAST of Thyroglobulin vs. Ensembl Xenopus
Match: amd1 (adenosylmethionine decarboxylase 1 [Source:Xenbase;Acc:XB-GENE-484611])

HSP 1 Score: 48.1358 bits (113), Expect = 2.990e-6
Identity = 31/110 (28.18%), Postives = 44/110 (40.00%), Query Frame = 2 
Query:  185 KSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQ----NSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATV 493
            K  ++  IP C  +G Y  +QC   +CWC +  G  I           C        ++Q     S  P    F   C  +G++  VQC    CFC D +G  +PG   V
Sbjct:  610 KRSSEIYIPACKEDGRYNDIQCSKAECWCVDDQGREIDRTRTQGTHPRCPTKCEKERYRQTAVRESLPPGAELFIPACDLEGHFLTVQCTGKLCFCVDLEGRIIPGTQKV 719

HSP 2 Score: 47.3654 bits (111), Expect = 5.496e-6
Identity = 27/87 (31.03%), Postives = 44/87 (50.57%), Query Frame = 2 
Query:  182 IKSMADTLIPRCDSNGNYAPVQC--QNGKCWCSNRDGITIHDYPDAKKKSHC-KCARASIEFKQNSDGPITAFFCKTDGNYGDVQCK 433
            I S + + IP+C  +G + PVQC  ++G+CWC + +G+ I+      K S C +  +       +  G  T   C  DG +  +QCK
Sbjct:  116 INSTSTSHIPQCKDSGEFEPVQCHRESGQCWCVDSEGMEIYGTRQTGKPSQCPRSCQVRGRRILHGFGEKTPPHCSADGTFQPIQCK 202
[Close]
BLAST of Thyroglobulin vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 46.595 bits (109), Expect = 9.669e-6
Identity = 45/165 (27.27%), Postives = 67/165 (40.61%), Query Frame = 2 
Query:   89 SFDCRPPKVCMSG------KCYANPLTTKCF----QIYGKAIKSMADTLIPRCDSNGNYAPVQCQ--NGKCWCSNRDG--ITIHDYPDAKKKSHCK-----------CARASIEFKQNSDG-PITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            SF CR    C SG      +C   PL T C     Q++ +  +      +P CD  GNY P+QC    G CWC ++ G  IT    P  +   +C            C R      ++  G P    +   C   G++  +QC   +  C+C D++G  + G  T
Sbjct:  870 SFSCR----CNSGYEGDGFQC-PEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPLCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRT 1029
[Close]
BLAST of Thyroglobulin vs. Ensembl Mouse
Match: Tg (thyroglobulin [Source:MGI Symbol;Acc:MGI:98733])

HSP 1 Score: 54.299 bits (129), Expect = 1.782e-8
Identity = 30/110 (27.27%), Postives = 53/110 (48.18%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHC--KC--ARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRK 511
             +P C + G+Y  +QC  G+CWC +  G  +        +  C  KC   RA ++   ++    ++FF   C  +G +  VQC  + C+C D +G  +PG  +   E ++
Sbjct:  616 FVPSCTAGGSYEDIQCYAGECWCVDSRGKELDGSRVRGGRPRCPTKCEKQRAQMQSLASAQPAGSSFFVPTCTREGYFLPVQCFNSECYCVDTEGQVIPGTQSTVGEAKQ 725

HSP 2 Score: 50.0618 bits (118), Expect = 6.147e-7
Identity = 31/105 (29.52%), Postives = 51/105 (48.57%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQNG--KCWCSNRDGITIHDYPDAKK-------KSHCKCARASIEFKQ--NSDGPITAFFCKTDGNYGDVQC--KQTTCFCTDEDGVRMPG 481
            +P+C  +G++  VQCQN    CWC + DG    + P +++        S C+  +  I      NS   +    C+  GNY  VQC  ++  C+C D +G+ + G
Sbjct:   50 VPQCSEDGSFQTVQCQNDGQSCWCVDSDG---REVPGSRQLGRPTVCLSFCQLHKQRILLGSYINSTDALYLPQCQDSGNYAPVQCDLQRVQCWCVDTEGMEVYG 151

HSP 3 Score: 48.521 bits (114), Expect = 1.792e-6
Identity = 31/95 (32.63%), Postives = 47/95 (49.47%), Query Frame = 2 
Query:  167 IYGKAIKSMADTLIPRCDSNGNYAPVQC--QNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQ----NSDGPITAFFCKTDGNYGDVQCK 433
            + G  I S     +P+C  +GNYAPVQC  Q  +CWC + +G+ +  Y   ++    +C R S E +     +  G  +   C  DG +  VQCK
Sbjct:  105 LLGSYINSTDALYLPQCQDSGNYAPVQCDLQRVQCWCVDTEGMEV--YGTRQQGRPTRCPR-SCEIRNRRLLHGVGDRSPPQCTADGEFMPVQCK 196
[Close]
BLAST of Thyroglobulin vs. UniProt/SwissProt
Match: sp|P06882|THYG_RAT (Thyroglobulin OS=Rattus norvegicus OX=10116 GN=Tg PE=1 SV=4)

HSP 1 Score: 57.7658 bits (138), Expect = 8.256e-9
Identity = 30/102 (29.41%), Postives = 50/102 (49.02%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIH------DYPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPG 481
             +P C + G+Y  +QC  G+CWC N  G  +        +P    K  C+  RA ++    +    ++FF   C ++G +  VQC  + C+C D +G  +PG
Sbjct:  616 FVPSCTAEGSYEDIQCYAGECWCVNSQGKEVEGSRVSGGHPRCPTK--CEKQRAQMQNLAGAQPAGSSFFVPTCTSEGYFLPVQCFNSECYCVDAEGQVIPG 715

HSP 2 Score: 54.299 bits (129), Expect = 1.623e-7
Identity = 33/108 (30.56%), Postives = 52/108 (48.15%), Query Frame = 2 
Query:  197 DTLIPRCDSNGNYAPVQCQNG--KCWCSNRDGITIHDYPDAKK-------KSHCKCARASIEFKQ--NSDGPITAFFCKTDGNYGDVQC--KQTTCFCTDEDGVRMPG 481
            D  +P+C  +G++  VQCQN    CWC + DG  +   P +++        S C+  +  I      NS   +    C+  GNY  VQC  +Q  C+C D +G+ + G
Sbjct:   47 DEYVPQCSEDGSFQTVQCQNDGQSCWCVDSDGTEV---PGSRQLGRPTACLSFCQLHKQRILLSSYINSTDALYLPQCQDSGNYAPVQCDLQQVQCWCVDTEGMEVYG 151
[Close]
BLAST of Thyroglobulin vs. UniProt/SwissProt
Match: sp|P01266|THYG_HUMAN (Thyroglobulin OS=Homo sapiens OX=9606 GN=TG PE=1 SV=5)

HSP 1 Score: 56.6102 bits (135), Expect = 2.547e-8
Identity = 30/103 (29.13%), Postives = 52/103 (50.49%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKK-------SHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPG 481
             +P C + G+Y  VQC +G+CWC N  G    + P ++ +       + C+  RA ++    S    +  F   C ++G++  VQC  + C+C D +G  +PG
Sbjct:  616 FVPSCTTEGSYEDVQCFSGECWCVNSWG---KELPGSRVRGGQPRCPTDCEKQRARMQSLMGSQPAGSTLFVPACTSEGHFLPVQCFNSECYCVDAEGQAIPG 715

HSP 2 Score: 48.9062 bits (115), Expect = 8.858e-6
Identity = 30/111 (27.03%), Postives = 51/111 (45.95%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQNG--KCWCSNRDGITIHDYPDAKKK----SHCKCARASIEFKQNSDGPITAFF--CKTDGNYGDVQC--KQTTCFCTDEDGVRMPGKATVDYEKR 508
            +P+C  +G++  VQCQN    CWC   +G  +       +     S C+  +  I      +   T++   C+  G+Y  VQC  +Q  C+C D +G+ + G   +   KR
Sbjct:   49 VPQCAEDGSFQTVQCQNDGRSCWCVGANGSEVLGSRQPGRPVACLSFCQLQKQQILLSGYINSTDTSYLPQCQDSGDYAPVQCDVQQVQCWCVDAEGMEVYGTRQLGRPKR 159
[Close]
BLAST of Thyroglobulin vs. UniProt/SwissProt
Match: sp|F1RRV3|THYG_PIG (Thyroglobulin OS=Sus scrofa OX=9823 GN=TG PE=1 SV=5)

HSP 1 Score: 54.6842 bits (130), Expect = 9.289e-8
Identity = 30/110 (27.27%), Postives = 49/110 (44.55%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCA-------RASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRK 511
             +P C + G+Y  VQC  G+CWC +  G  +        +  C  A         S+  +Q +   +    C  +G++  VQC  + C+C D DG  +PG  T   E ++
Sbjct:  617 FVPSCTAEGSYEDVQCFAGECWCVDARGRELAGSRARGGRPRCPTACEKQRERMQSLLGRQPAGSSVFVPSCTREGHFLPVQCFSSDCYCVDADGQPIPGTRTAPGEPKQ 726
[Close]
BLAST of Thyroglobulin vs. UniProt/SwissProt
Match: sp|O08710|THYG_MOUSE (Thyroglobulin OS=Mus musculus OX=10090 GN=Tg PE=1 SV=3)

HSP 1 Score: 54.299 bits (129), Expect = 1.246e-7
Identity = 30/110 (27.27%), Postives = 53/110 (48.18%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHC--KC--ARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRK 511
             +P C + G+Y  +QC  G+CWC +  G  +        +  C  KC   RA ++   ++    ++FF   C  +G +  VQC  + C+C D +G  +PG  +   E ++
Sbjct:  616 FVPSCTAGGSYEDIQCYAGECWCVDSRGKELDGSRVRGGRPRCPTKCEKQRAQMQSLASAQPAGSSFFVPTCTREGYFLPVQCFNSECYCVDTEGQVIPGTQSTVGEAKQ 725

HSP 2 Score: 50.0618 bits (118), Expect = 4.300e-6
Identity = 31/105 (29.52%), Postives = 51/105 (48.57%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQNG--KCWCSNRDGITIHDYPDAKK-------KSHCKCARASIEFKQ--NSDGPITAFFCKTDGNYGDVQC--KQTTCFCTDEDGVRMPG 481
            +P+C  +G++  VQCQN    CWC + DG    + P +++        S C+  +  I      NS   +    C+  GNY  VQC  ++  C+C D +G+ + G
Sbjct:   50 VPQCSEDGSFQTVQCQNDGQSCWCVDSDG---REVPGSRQLGRPTVCLSFCQLHKQRILLGSYINSTDALYLPQCQDSGNYAPVQCDLQRVQCWCVDTEGMEVYG 151
[Close]
BLAST of Thyroglobulin vs. UniProt/SwissProt
Match: sp|P01267|THYG_BOVIN (Thyroglobulin OS=Bos taurus OX=9913 GN=TG PE=1 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.246e-7
Identity = 31/110 (28.18%), Postives = 51/110 (46.36%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDY----PDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRK 511
             +P C + G+Y  VQC  G CWC +  G  +          +  + C+  RA ++    S    ++ F   C + GN+  VQC  + C+C D +G  +PG  +   E +K
Sbjct:  615 FVPACTAEGSYEEVQCFAGDCWCVDAQGRELAGSRVRGGRPRCPTECEKQRARMQSLLGSQPAGSSLFVPACTSKGNFLPVQCFNSECYCVDTEGQPIPGTRSALGEPKK 724
[Close]
BLAST of Thyroglobulin vs. TrEMBL
Match: A0A194AKI3 (Uncharacterized protein OS=Pinctada fucata OX=50426 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 7.439e-11
Identity = 45/137 (32.85%), Postives = 66/137 (48.18%), Query Frame = 2 
Query:  134 YANPLTTKCFQIYGKAIKSMAD---------------TLI----PRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPD---AKKKSHCKCARASIEFKQNSDGPITAFF-CKTDGNYGDVQCKQTTCFCTDEDGVRM 475
            + +P T KC Q +G  + S  D               +L+    P CD  G Y P QCQ  +C+C +++G  I  Y       ++  C+CAR   E+   + G I  FF C +DG+Y   QC+   CFC D +GV+M
Sbjct:   86 HCDPKTLKCVQGFGGLLLSRRDDAPCAAALLKAQNGPSLLGAPTPSCDGEGYYQPKQCQGSQCYCVSKNGKEISGYTANVWEAQQMTCQCARDQAEYM--ASGLIGKFFYCTSDGSYQTYQCQGDVCFCADTNGVKM 220
[Close]
BLAST of Thyroglobulin vs. TrEMBL
Match: A7S7C3 (Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g107313 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.257e-10
Identity = 42/133 (31.58%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  122 SGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIHD------YPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPG 481
            S +    P   + F  YG +  + AD  +PRCD++G Y PVQC +  G CWC ++DGI I         P  K+  +  C R        +   +T  +   C+ DG+Y  VQC   +  C+C D  G  + G
Sbjct:   47 SARVGLTPCQKQRFNAYGVSESTPADRYVPRCDASGGYEPVQCLSTTGHCWCVDKDGIEIRGTRRRGVRPVCKQGYNTPCERERYRALGWTGLRVTGLWVPDCRPDGSYESVQCHALSGYCWCVDRSGHELFG 179
[Close]
BLAST of Thyroglobulin vs. TrEMBL
Match: A0A1S3J5E7 (equistatin-like OS=Lingula unguis OX=7574 GN=LOC106170269 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 4.748e-10
Identity = 37/106 (34.91%), Postives = 53/106 (50.00%), Query Frame = 2 
Query:  209 PRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPD---AKKKSHCKCARASIEFKQNSDGPI-TAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT 514
            P CD++G Y+  QC+   C+C N+DG  + DY       K   C CAR   E+++   G I   + CK +GNY   QC  + C+C DE G ++     V     KT
Sbjct:   26 PTCDADGTYSAKQCKEASCFCVNKDGDRLGDYSARFWEAKDMTCNCARDEDEYQKK--GLIGKMYICKENGNYEKYQCTGSVCYCVDEKGSKLESTPPVSISAVKT 129
[Close]
BLAST of Thyroglobulin vs. TrEMBL
Match: A0A2T7PUS9 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04132 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 8.542e-10
Identity = 36/96 (37.50%), Postives = 51/96 (53.12%), Query Frame = 2 
Query:  203 LIPRCDSNGN--YAPVQCQNGKCWCSNRDGITIHDYPDA--KKKSHCKCARASIEF-KQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRM 475
             +PRCD+  +  Y+P+QC+   C+C + DG  + DY +      S+C+CAR   EF K    G I  F C  DG Y   +C  + CFC   +G RM
Sbjct:   40 FLPRCDAGNSALYSPIQCRGSACYCVSSDGTMLSDYSNIPFGTPSNCECARKEYEFSKARMKGQI--FNCAPDGTYSPTRCYGSRCFCVYRNGARM 133
[Close]
BLAST of Thyroglobulin vs. TrEMBL
Match: A0A3Q3J0V8 (Uncharacterized protein OS=Monopterus albus OX=43700 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.496e-9
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 2 
Query:  134 YANPLTTKCFQIYGKAIKS--------MADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKK----SHCKCARA-SIEFKQN--SDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRM 475
            + +PL   CFQ+    ++S         A   +P C S+G +  VQCQ+G+CWC N  G  +       ++    SHC+  RA +++ K N  +   I    C  DG++  +QC  + CFC D +G  M
Sbjct:   11 FVSPLC--CFQVLIPLLRSCSSEDKDDAAAIFVPSCTSSGGFQEVQCQSGECWCVNPHGQEVSGSRAVGRRPRCPSHCERERAMALKVKSNMAAGAEIHIPACSEDGDFLPLQCVGSRCFCVDAEGNTM 137
[Close]
BLAST of Thyroglobulin vs. Ensembl Cavefish
Match: tg (thyroglobulin [Source:NCBI gene;Acc:103037495])

HSP 1 Score: 59.6918 bits (143), Expect = 2.300e-10
Identity = 36/119 (30.25%), Postives = 55/119 (46.22%), Query Frame = 2 
Query:  143 PLTTKCFQIYG--KAIKSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHC--KCARA---SIEFKQNSDGPITAFF--CKTDGNYGDVQCKQTTCFCTDEDGVR 472
            PL    FQ +    +  S +   +P+C  +G Y  VQCQ  +CWC++  G  +        +  C  +C +    +I  K +S      F   C+ DG Y  +QC   +CFC D+ GVR
Sbjct:  603 PLFQAVFQNFDVCSSPASPSSLYLPQCTEDGQYQAVQCQGSECWCADSQGQEVMGSRSIGYRPRCPSRCEKERMMAIAVKASSSAGSEVFIPKCEEDGEYVSLQCLGKSCFCLDQRGVR 721
[Close]
BLAST of Thyroglobulin vs. Ensembl Sea Lamprey
Match: tg (thyroglobulin [Source:ZFIN;Acc:ZDB-GENE-030519-1])

HSP 1 Score: 47.3654 bits (111), Expect = 8.107e-7
Identity = 31/102 (30.39%), Postives = 39/102 (38.24%), Query Frame = 2 
Query:  203 LIPRCDSNGN-YAPVQCQNGKCWCSNRDGITIHDY-----PDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTTCFCTDEDGVRMPG 481
             + RC  +G  Y PVQC    CWC +  G  I           +  S C+  RA     +      T  F   C   G+Y  VQC    CFC    G  +PG
Sbjct:  606 FVARCGEDGGAYEPVQCHGAACWCVDAHGREIAGTRAVGGAPPRCPSACEGERARALLARRGRPVGTPLFVPECDGAGDYRPVQCSGDRCFCVGAGGAEVPG 707
[Close]
BLAST of Thyroglobulin vs. Ensembl Nematostella
Match: EDO40372 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7C3])

HSP 1 Score: 66.2402 bits (160), Expect = 5.887e-14
Identity = 42/133 (31.58%), Postives = 62/133 (46.62%), Query Frame = 2 
Query:  122 SGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIHD------YPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPG 481
            S +    P   + F  YG +  + AD  +PRCD++G Y PVQC +  G CWC ++DGI I         P  K+  +  C R        +   +T  +   C+ DG+Y  VQC   +  C+C D  G  + G
Sbjct:   47 SARVGLTPCQKQRFNAYGVSESTPADRYVPRCDASGGYEPVQCLSTTGHCWCVDKDGIEIRGTRRRGVRPVCKQGYNTPCERERYRALGWTGLRVTGLWVPDCRPDGSYESVQCHALSGYCWCVDRSGHELFG 179

HSP 2 Score: 47.3654 bits (111), Expect = 4.910e-7
Identity = 33/106 (31.13%), Postives = 52/106 (49.06%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQN--GKCWCSNRD-----GITIHDYPDAKKK---SHCKCARASIEFKQNSDGPITAFF--CKTDGNYGDVQCKQTT--CFCTDEDGVRMPG 481
            +PRC+S+G+++ VQC    G CWC +R      G  +   PD   +   + C+  R +  +  +   P   +   C   G Y  VQC  TT  C+C D+DG+ + G
Sbjct:    4 VPRCESDGSFSSVQCNELTGYCWCVDRQGNPVFGTEVRGQPDCSARVGLTPCQKQRFNA-YGVSESTPADRYVPRCDASGGYEPVQCLSTTGHCWCVDKDGIEIRG 108
[Close]
BLAST of Thyroglobulin vs. Ensembl Nematostella
Match: EDO40370 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7C1])

HSP 1 Score: 57.7658 bits (138), Expect = 9.835e-11
Identity = 40/125 (32.00%), Postives = 56/125 (44.80%), Query Frame = 2 
Query:  152 TKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQC--QNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQ---------NSDGPITAFF--CKTDGNYGDVQCKQTT--CFCTDEDGVRMPG 481
            T C +  G+A+ S     +P C  +G+Y+ VQC    G+CWCS+ DG    + P  +     +C        Q             P+ A+   C  DG+Y  VQC   T  C+C DEDG   PG
Sbjct:   73 TPCQRAKGEALLSPETKRVPNCRPDGSYSRVQCDKSTGECWCSSEDG---SETPGTRTSGTLRCPANEFSACQKHRHRVQGMTGQAPVGAYVPRCADDGSYETVQCHDGTRYCWCVDEDGKERPG 194

HSP 2 Score: 53.1434 bits (126), Expect = 4.049e-9
Identity = 36/123 (29.27%), Postives = 55/123 (44.72%), Query Frame = 2 
Query:  164 QIYGKAI-KSMADTLIPRCDSNGNYAPVQCQNGK--CWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFF---------CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            ++ G A+ +   +  IP C S+G Y  VQC  G   CWC +  G+ +      + ++  +CA    E          A           C+ DG+Y  VQC ++T  C+C+ EDG   PG  T
Sbjct:    8 RLLGTALGRGNVEGYIPHCKSDGRYEEVQCHTGTKYCWCVDEKGVEVW---GTRTRTFIRCAAFVKEPTPCQRAKGEALLSPETKRVPNCRPDGSYSRVQCDKSTGECWCSSEDGSETPGTRT 127
[Close]
BLAST of Thyroglobulin vs. Ensembl Nematostella
Match: EDO47796 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL42])

HSP 1 Score: 55.0694 bits (131), Expect = 9.503e-10
Identity = 40/128 (31.25%), Postives = 59/128 (46.09%), Query Frame = 2 
Query:  152 TKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQN--GKCWCSNRDGITIHDYP---------DAKKKSHCKCARAS--IEFKQNSDGPITAFFCKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            T C Q    +I+S      PRC  +G +  +QC +  G CWC +  G TI +           +A   S C  AR S  I  +     P+    C+ DG++ +VQC   T  C+C D +G+ +PG  T
Sbjct:   70 TVCQQQVRYSIQSPLGGNAPRCKEDGRFEEIQCDHVTGFCWCVDDSG-TIKEGTKTNGTIACGNATPLSRCHQARQSIFIAIEHAQSAPLFIPTCRDDGSFAEVQCHGLTGQCWCVDMNGIEIPGSVT 196

HSP 2 Score: 46.9802 bits (110), Expect = 8.617e-7
Identity = 32/121 (26.45%), Postives = 55/121 (45.45%), Query Frame = 2 
Query:  176 KAIKSMADTL--IPRCDSNGNYAPVQCQ--NGKCWCSNRDGITIHDYPDAKKKSHCKCARASI--------EFKQNSDGPI--TAFFCKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            ++ KS +D    +P+C   G+Y  VQC    G CWC + +G    +   ++ + H  C   ++        + + +   P+   A  CK DG + ++QC   T  C+C D+ G    G  T
Sbjct:    7 RSTKSSSDVTDHVPQCKKTGHYEEVQCSAWTGYCWCVDTNGT---EMAGSRTREHLSCPSLAVSSLTVCQQQVRYSIQSPLGGNAPRCKEDGRFEEIQCDHVTGFCWCVDDSGTIKEGTKT 124
[Close]
BLAST of Thyroglobulin vs. Ensembl Nematostella
Match: EDO40371 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7C2])

HSP 1 Score: 53.9138 bits (128), Expect = 1.842e-9
Identity = 34/111 (30.63%), Postives = 52/111 (46.85%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQNGK--CWCSNRDGITIHDYPDAKKKSHCKC-------ARASIEFKQNSDGPITAFF--CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKATVDY 499
            +PRC  +G+Y  VQC +G   CWC + DG    + P  ++     C        RA +E    +   +  F   C  DG Y DVQC+++T  C+C D  G  + G  + + 
Sbjct:   11 VPRCADDGSYETVQCHDGTRYCWCVDEDG---KERPGTRQTGQPNCDPVPVTPCRAQVEQALKTPASLDRFVPRCTLDGAYEDVQCQESTGECWCVDAQGSELDGTRSTEL 118

HSP 2 Score: 48.1358 bits (113), Expect = 2.412e-7
Identity = 41/146 (28.08%), Postives = 65/146 (44.52%), Query Frame = 2 
Query:  107 PKVCMSGKCYANPL-TTKCFQIYGKAIKSMA--DTLIPRCDSNGNYAPVQCQN--GKCWCSNRDG-----------ITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQT--TCFCTDEDGVRMPG 481
            P    +G+   +P+  T C     +A+K+ A  D  +PRC  +G Y  VQCQ   G+CWC +  G           +T H     K  S   C +  ++    S   +   F   C++DG+Y  +QC ++   C+C D  G  + G
Sbjct:   43 PGTRQTGQPNCDPVPVTPCRAQVEQALKTPASLDRFVPRCTLDGAYEDVQCQESTGECWCVDAQGSELDGTRSTELVTCH-----KHMSPSVCQQDRMQALAWSGRLVVGAFIPRCRSDGSYDPIQCHESPGQCWCVDVHGNELTG 183
[Close]
BLAST of Thyroglobulin vs. Ensembl Nematostella
Match: EDO38469 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCV6])

HSP 1 Score: 52.373 bits (124), Expect = 7.578e-9
Identity = 37/106 (34.91%), Postives = 48/106 (45.28%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQN--GKCWCSNRD------GITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPG 481
             +P C S G +A VQC    G CWC++ D      G      PD    +  KC R    ++ N   P    F   CK DGNY  +QC  +T  C+C D DG  + G
Sbjct:   76 FVPLCTSEGKFADVQCHGSTGYCWCAHPDNGQEVTGTRQRGQPDCSDVALSKCQR---HYRDNLIYPPIGRFLPTCKPDGNYEKMQCHGSTGFCWCVDADGAELVG 178

HSP 2 Score: 43.8986 bits (102), Expect = 9.087e-6
Identity = 29/95 (30.53%), Postives = 43/95 (45.26%), Query Frame = 2 
Query:  203 LIPRCDSNGNYAPVQCQN--GKCWCSNRDGIT-----IHDYPDAKKKSHCKCA-RASIEFKQNSDGPIT----AFFCKTDGNYGDVQCKQTTCFC 451
             +P+C  NGNY  VQC    G CWC + DG+      I   P+       K   ++ +E     DG  +       C ++G + DVQC  +T +C
Sbjct:    4 FLPKCKPNGNYDEVQCHGSTGFCWCVDMDGVELLGSKIRGPPNCTALGANKTVCQSQLEAALGPDGIASPGRFVPLCTSEGKFADVQCHGSTGYC 98
[Close]
BLAST of Thyroglobulin vs. Ensembl Medaka
Match: ENSORLT00000046650.1 (pep primary_assembly:ASM223467v1:16:31876438:31883319:-1 gene:ENSORLG00000030206.1 transcript:ENSORLT00000046650.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 4.667e-7
Identity = 28/107 (26.17%), Postives = 47/107 (43.93%), Query Frame = 2 
Query:  176 KAIKSMADTLIPRCDSNGNYAPVQCQNG-KCWCSNRDGITIHDYPDAKKKSH-CKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKAT 490
            +A    +   +P C+  G+Y+  QCQ G +CWC +  G    + P  ++      C   +     N    +    C  DG++  +QC  T+C+C  E G  + G  T
Sbjct:   48 RAALETSSVFVPTCERGGSYSFTQCQQGGQCWCVDPSG---REVPGTRQLGGPLACGEKNHPLIANMAAGLQRRQCGPDGSFAPLQCDVTSCWCVTEYGQEVVGTRT 151
[Close]
BLAST of Thyroglobulin vs. Ensembl Medaka
Match: nid2a (nidogen 2 [Source:NCBI gene;Acc:101173773])

HSP 1 Score: 46.9802 bits (110), Expect = 5.207e-6
Identity = 35/117 (29.91%), Postives = 47/117 (40.17%), Query Frame = 2 
Query:  206 IPRCDSNGNYAPVQCQ--NGKCWC-----SNRDGITIH--------DYPDA--KKKSHCKCARASIEFKQNSDGPITAFF---CKTDGNYGDVQCKQTT--CFCTDEDGVRMPGKAT 490
            +P+CD NG Y P QC    G CWC       R G              PD   + K+ C+  R S+        P+   +   C  DG+Y   QC  ++  C+C D  G   PG  T
Sbjct:  944 VPQCDQNGEYVPQQCHTSTGYCWCVDGMGQERSGTRSAPGSPSMDCSKPDEALQPKTPCERLRESVLASSAGGRPLAGAYAPQCDEDGHYVPRQCHGSSGYCWCVDSSGQERPGTRT 1060
[Close]
BLAST of Thyroglobulin vs. Planmine SMEST
Match: SMESG000017039.1 (SMESG000017039.1)

HSP 1 Score: 338.961 bits (868), Expect = 9.283e-121
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 2 
Query:   26 MNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT 514
            MNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT
Sbjct:    1 MNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT 163
[Close]
BLAST of Thyroglobulin vs. Planmine SMEST
Match: SMESG000017161.1 (SMESG000017161.1)

HSP 1 Score: 122.479 bits (306), Expect = 1.579e-35
Identity = 66/161 (40.99%), Postives = 92/161 (57.14%), Query Frame = 2 
Query:   26 MNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMA-DTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEK 505
            MN    CVF+ L+ A  GC DS  C   +VC+SG C  NPLTTKC+Q Y K +K++   + IP C +NG Y PVQC    C+CS+ DG  I +Y +  + S+CKC      +  N    I  F C  +G+Y  +QC  ++C+C D  G ++PG  +V  EK
Sbjct:    1 MNSVIGCVFLALIHAAFGCNDSKGCTDLEVCVSGNCSKNPLTTKCYQEYDKVMKNLLFGSFIPCCKTNGIYCPVQCPTNICYCSSPDGKKISNYFNINENSNCKCGLDKYLYSLNMAVGII-FNCNENGSYRPIQCLGSSCYCVDAQGKKIPGTISVHIEK 160
[Close]
BLAST of Thyroglobulin vs. Planmine SMEST
Match: SMESG000017140.1 (SMESG000017140.1)

HSP 1 Score: 110.923 bits (276), Expect = 4.531e-31
Identity = 63/166 (37.95%), Postives = 91/166 (54.82%), Query Frame = 2 
Query:   26 MNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKSMADTL---IPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKSHCKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT 514
            MN   IC+F+ LV+A+ GCEDS DCR P  C  G+C     TT+CF+   +A       +   IP CD+ GNY+PVQC    C+CS+RDG  +         S+C+CA    EF  N++ P   + C+ +GN+  +QC+   CFC D +G  +  K +V    + T
Sbjct:    1 MNAIIICIFLFLVQASLGCEDSIDCRYPMFCQGGRC-----TTRCFEEAKRAKAQCGKRVECPIPDCDAFGNYSPVQCLYHMCFCSSRDGKQLTKPFIPHDSSNCRCAVIKYEFAHNNN-PEVKYACQENGNFKPLQCEGGNCFCVDLNGRVIKDKKSVPVLDKDT 160
[Close]
BLAST of Thyroglobulin vs. Planmine SMEST
Match: SMESG000016977.1 (SMESG000016977.1)

HSP 1 Score: 104.375 bits (259), Expect = 2.242e-28
Identity = 60/169 (35.50%), Postives = 90/169 (53.25%), Query Frame = 2 
Query:   23 IMNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKS-MADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYP---DAKKKSHCKCARASIEFKQNSDGPITAFF-CKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT 514
            I++   I   +L+ +    C+DS DC    +C+  +C  NPL T+CF    KA K  +    IP+CDSNG++ PVQC   KC+C +R+G  I +Y    +      CKCAR   ++     G I   F C T+G+Y  VQC  + CFC D +G  + GK  V+  + ++
Sbjct:    5 ILSLITIMNLLLINKVYGTCQDSSDCNNDLICIGNECSDNPLNTQCFNKLSKARKGGLLGAPIPKCDSNGDFLPVQCTGSKCYCVDREGNNIRNYAAHINEFANMDCKCAREQNDYFL--TGLIGKMFSCTTNGSYSSVQCNGSVCFCADYNGKAVAGKPIVNIGQIQS 171
[Close]
BLAST of Thyroglobulin vs. Planmine SMEST
Match: SMESG000016977.1 (SMESG000016977.1)

HSP 1 Score: 103.605 bits (257), Expect = 3.642e-28
Identity = 60/160 (37.50%), Postives = 87/160 (54.37%), Query Frame = 2 
Query:   53 ILLVEATRG-CEDSFDCRPPKVCMSGKCYANPLTTKCFQIYGKAIKS-MADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYP---DAKKKSHCKCARASIEFKQNSDGPITAFF-CKTDGNYGDVQCKQTTCFCTDEDGVRMPGKATVDYEKRKT 514
            +LL+    G C+DS DC    +C+  +C  NPL T+CF    KA K  +    IP+CDSNG++ PVQC   KC+C +R+G  I +Y    +      CKCAR   ++     G I   F C T+G+Y  VQC  + CFC D +G  + GK  V+  + ++
Sbjct:    3 LLLINKVYGTCQDSSDCNNDLICIGNECSDNPLNTQCFNKLSKARKGGLLGAPIPKCDSNGDFLPVQCTGSKCYCVDREGNNIRNYAAHINEFANMDCKCAREQNDYFLT--GLIGKMFSCTTNGSYSSVQCNGSVCFCADYNGKAVAGKPIVNIGQIQS 160
The following BLAST results are available for this feature:
BLAST of Thyroglobulin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
TG5.304e-929.13thyroglobulin [Source:HGNC Symbol;Acc:HGNC:11764][more]
CD748.409e-630.91CD74 molecule [Source:HGNC Symbol;Acc:HGNC:1697][more]
CD749.139e-630.91CD74 molecule [Source:HGNC Symbol;Acc:HGNC:1697][more]
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BLAST of Thyroglobulin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thyroglobulin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thyroglobulin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tg1.157e-929.91thyroglobulin [Source:ZFIN;Acc:ZDB-GENE-030519-1][more]
nid2b9.963e-827.67nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2a3.274e-729.66nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2a1.869e-631.03nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2b1.959e-627.67nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of Thyroglobulin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
nid24.194e-828.06nidogen 2 [Source:NCBI gene;Acc:100036716][more]
nid28.553e-828.67nidogen 2 [Source:NCBI gene;Acc:100036716][more]
amd12.990e-628.18adenosylmethionine decarboxylase 1 [Source:Xenbase... [more]
nid29.669e-627.27nidogen 2 [Source:NCBI gene;Acc:100036716][more]
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BLAST of Thyroglobulin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Tg1.782e-827.27thyroglobulin [Source:MGI Symbol;Acc:MGI:98733][more]
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BLAST of Thyroglobulin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P06882|THYG_RAT8.256e-929.41Thyroglobulin OS=Rattus norvegicus OX=10116 GN=Tg ... [more]
sp|P01266|THYG_HUMAN2.547e-829.13Thyroglobulin OS=Homo sapiens OX=9606 GN=TG PE=1 S... [more]
sp|F1RRV3|THYG_PIG9.289e-827.27Thyroglobulin OS=Sus scrofa OX=9823 GN=TG PE=1 SV=... [more]
sp|O08710|THYG_MOUSE1.246e-727.27Thyroglobulin OS=Mus musculus OX=10090 GN=Tg PE=1 ... [more]
sp|P01267|THYG_BOVIN1.246e-728.18Thyroglobulin OS=Bos taurus OX=9913 GN=TG PE=1 SV=... [more]
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BLAST of Thyroglobulin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A194AKI37.439e-1132.85Uncharacterized protein OS=Pinctada fucata OX=5042... [more]
A7S7C34.257e-1031.58Predicted protein (Fragment) OS=Nematostella vecte... [more]
A0A1S3J5E74.748e-1034.91equistatin-like OS=Lingula unguis OX=7574 GN=LOC10... [more]
A0A2T7PUS98.542e-1037.50Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A3Q3J0V81.496e-931.01Uncharacterized protein OS=Monopterus albus OX=437... [more]
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BLAST of Thyroglobulin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tg2.300e-1030.25thyroglobulin [Source:NCBI gene;Acc:103037495][more]
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BLAST of Thyroglobulin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tg8.107e-730.39thyroglobulin [Source:ZFIN;Acc:ZDB-GENE-030519-1][more]
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BLAST of Thyroglobulin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thyroglobulin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO403725.887e-1431.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO403709.835e-1132.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO477969.503e-1031.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO403711.842e-930.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO384697.578e-934.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Thyroglobulin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000046650.14.667e-726.17pep primary_assembly:ASM223467v1:16:31876438:31883... [more]
nid2a5.207e-629.91nidogen 2 [Source:NCBI gene;Acc:101173773][more]
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BLAST of Thyroglobulin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017039.19.283e-121100.00SMESG000017039.1[more]
SMESG000017161.11.579e-3540.99SMESG000017161.1[more]
SMESG000017140.14.531e-3137.95SMESG000017140.1[more]
SMESG000016977.12.242e-2835.50SMESG000016977.1[more]
SMESG000016977.13.642e-2837.50SMESG000016977.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030687 ID=SMED30030687|Name=Thyroglobulin|organism=Schmidtea mediterranea sexual|type=transcript|length=566bp
TTTAATGTCAGTTGTCGTTTGAATAATGAATTATAGACCTATTTGCGTAT
TTATATTGTTGGTTGAAGCAACACGTGGTTGCGAGGATTCATTTGATTGT
AGACCGCCAAAGGTTTGCATGTCAGGTAAGTGCTATGCAAATCCCTTGAC
CACTAAATGCTTCCAGATTTATGGTAAGGCAATAAAGTCAATGGCTGATA
CCTTGATTCCTCGATGTGACTCCAATGGCAATTATGCGCCAGTCCAATGT
CAAAATGGCAAATGCTGGTGCTCAAACAGAGATGGGATAACAATTCATGA
TTATCCTGATGCAAAGAAAAAATCTCATTGCAAGTGTGCTCGAGCCTCAA
TTGAGTTTAAGCAAAACTCCGATGGACCAATAACTGCATTTTTCTGTAAA
ACGGACGGCAATTATGGGGACGTGCAATGCAAACAAACAACTTGCTTTTG
TACTGACGAAGACGGTGTTAGAATGCCAGGAAAAGCAACGGTAGACTATG
AAAAAAGGAAAACACTTCTGTGCAAAAACAATTAATATTATCTTCTCCTT
AACTTTTAATTGCCAA
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protein sequence of SMED30030687-orf-1

>SMED30030687-orf-1 ID=SMED30030687-orf-1|Name=SMED30030687-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=170bp
MNYRPICVFILLVEATRGCEDSFDCRPPKVCMSGKCYANPLTTKCFQIYG
KAIKSMADTLIPRCDSNGNYAPVQCQNGKCWCSNRDGITIHDYPDAKKKS
HCKCARASIEFKQNSDGPITAFFCKTDGNYGDVQCKQTTCFCTDEDGVRM
PGKATVDYEKRKTLLCKNN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR036857Thyroglobulin_1_sf
IPR000716Thyroglobulin_1
Vocabulary: biological process
TermDefinition
GO:0045056transcytosis
GO:0006590thyroid hormone generation
GO:0009268response to pH
GO:0032496response to lipopolysaccharide
GO:0042446hormone biosynthetic process
Vocabulary: cellular component
TermDefinition
GO:0048471perinuclear region of cytoplasm
GO:0043234protein complex
GO:0005576extracellular region
GO:0005615extracellular space
GO:0005783endoplasmic reticulum
GO:0005794Golgi apparatus
Vocabulary: molecular function
TermDefinition
GO:0051087chaperone binding
GO:0042803protein homodimerization activity
GO:0043168anion binding
GO:0005102signaling receptor binding
GO:0005179hormone activity
GO:0005515protein binding
GO:0032403protein complex binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000716Thyroglobulin type-1SMARTSM00211TY_2coord: 61..108
e-value: 6.3E-7
score: 39.0
coord: 121..166
e-value: 0.49
score: 7.4
IPR000716Thyroglobulin type-1PFAMPF00086Thyroglobulin_1coord: 54..102
e-value: 9.2E-12
score: 44.5
coord: 124..157
e-value: 2.4E-7
score: 30.3
IPR000716Thyroglobulin type-1PROSITEPS51162THYROGLOBULIN_1_2coord: 42..102
score: 9.565
IPR000716Thyroglobulin type-1PROSITEPS51162THYROGLOBULIN_1_2coord: 124..166
score: 6.695
IPR000716Thyroglobulin type-1CDDcd00191TYcoord: 61..102
e-value: 1.32174E-8
score: 47.0733
IPR036857Thyroglobulin type-1 superfamilyGENE3DG3DSA:4.10.800.10coord: 42..103
e-value: 1.8E-12
score: 48.4
coord: 104..163
e-value: 7.4E-6
score: 27.2
IPR036857Thyroglobulin type-1 superfamilySUPERFAMILYSSF57610Thyroglobulin type-1 domaincoord: 25..105
IPR036857Thyroglobulin type-1 superfamilySUPERFAMILYSSF57610Thyroglobulin type-1 domaincoord: 124..157
NoneNo IPR availablePANTHERPTHR12352SECRETED MODULAR CALCIUM-BINDING PROTEINcoord: 24..162
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.537