SMED30030536
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030536 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30030536 vs. TrEMBL
Match: G7YF65 (Calcium-activated potassium channel subunit beta-2 OS=Clonorchis sinensis OX=79923 GN=CLF_106446 PE=4 SV=1) HSP 1 Score: 100.138 bits (248), Expect = 2.638e-22 Identity = 66/181 (36.46%), Postives = 100/181 (55.25%), Query Frame = 2 Query: 74 YRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFI---TNKDNQTAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP-GIFMIFTLISSVLF 604 YR LK+ C + IVECI A+L V PY++E F ++C +++ + + A + CENR +KD RFPC+ + + + N T IL++ I T+ QYK F C+T C + N ++ R+ +N F CY + + +EALL K S K L ++ WP G+F FT I+ VLF Sbjct: 12 YRVACLKVLYLTCATVITTTFIVECILAHLVVQPYLHESAFTLTNCSYVHAHIVNHAVK-CENRCSKDRSRFPCLQVLVRYTSRGANHTVILFDNIATYHQYKAFGCATSSCHHRDADNTAYVLRFEHRIKHRNHFECYIHSVHENEALLTKFYSAKTLFHVIFWPVGLF--FTSIACVLF 189
BLAST of SMED30030536 vs. TrEMBL
Match: A0A075AHQ6 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_13199 PE=4 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 8.552e-22 Identity = 65/181 (35.91%), Postives = 100/181 (55.25%), Query Frame = 2 Query: 74 YRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFI---TNKDNQTAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP-GIFMIFTLISSVLF 604 YR LK+ C + IVECI A+L V PY++E F ++C +++ + + A + CENR +KD RFPC+ + + + N T IL++ I T+ QYK F C+T C + N ++ R+ +N F CY + + +EALL K S + L ++ WP G+F FT I+ VLF Sbjct: 44 YRVGCLKVLYLTCATVITTTFIVECILAHLVVQPYLHESAFTLTNCSYVHAHIVNHAVK-CENRCSKDRSRFPCLQVLVRYTSRGANHTVILFDNIATYHQYKAFGCATSSCHHRDAENTAYVLRFEHRIKHRNHFECYIHSVHENEALLTKFYSARTLFHVIFWPVGLF--FTSIACVLF 221
BLAST of SMED30030536 vs. TrEMBL
Match: A0A3R7CBH0 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_9879s PE=4 SV=1) HSP 1 Score: 99.3673 bits (246), Expect = 1.816e-21 Identity = 66/181 (36.46%), Postives = 100/181 (55.25%), Query Frame = 2 Query: 74 YRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFI---TNKDNQTAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP-GIFMIFTLISSVLF 604 YR LK+ C + IVECI A+L V PY++E F ++C +++ + + A + CENR +KD RFPC+ + + + N T IL++ I T+ QYK F C+T C + N ++ R+ +N F CY + + +EALL K S K L ++ WP G+F FT I+ VLF Sbjct: 63 YRVACLKVLYLTCATVITTTFIVECILAHLVVQPYLHESAFTLTNCSYVHAHIVNHAVK-CENRCSKDRSRFPCLQVLVRYTSRGANHTVILFDNIATYHQYKAFGCATSSCHHRDADNTAYVLRFEHRIKHRNHFECYIHSVHENEALLTKFYSAKTLFHVIFWPVGLF--FTSIACVLF 240
BLAST of SMED30030536 vs. TrEMBL
Match: A0A4S2KQU4 (Uncharacterized protein (Fragment) OS=Opisthorchis felineus OX=147828 GN=CRM22_010674 PE=4 SV=1) HSP 1 Score: 96.2857 bits (238), Expect = 1.752e-20 Identity = 63/181 (34.81%), Postives = 100/181 (55.25%), Query Frame = 2 Query: 74 YRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFI---TNKDNQTAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP-GIFMIFTLISSVLF 604 YR LK+ C + IVECI A+L V PY++E F ++C +++ + + A + CENR +KD RFPC+ + + + N T IL++ I T+ QYK + C+T C + N ++ R+ +N F CY + + +EALL K S + L ++ WP G+F+ T I+ VLF Sbjct: 47 YRVGCLKVLYLTCATVITTTFIVECILAHLVVQPYLHESAFTLTNCSYVHAHIVNHAVK-CENRCSKDRSRFPCLQVLVRYTSRGANHTVILFDNIATYHQYKAYGCATSSCHHRDADNTAYVLRFEHRIKHRNHFECYIHSVHENEALLTKFYSARTLFHVIFWPVGLFL--TSIACVLF 224
BLAST of SMED30030536 vs. TrEMBL
Match: A0A4E0RIE4 (Calcium activated potassium channel subunit OS=Fasciola hepatica OX=6192 GN=D915_001494 PE=4 SV=1) HSP 1 Score: 95.1301 bits (235), Expect = 2.430e-20 Identity = 52/154 (33.77%), Postives = 79/154 (51.30%), Query Frame = 2 Query: 110 CLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFI---TNKDNQTAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP 562 C + I+ECI +L V PY++E F ++SC +L+ + + CEN+ +KD RFPC+ + + T DN T +LY+ I T+ YK C+T C + N W+ R+ Q F CY + + EALLFK S + + WP Sbjct: 26 CATVITTSFIIECILVHLVVQPYIHESAFSHTSCTYLHSDIIRQNVK-CENKCSKDRSRFPCLKVLVSYYTGGDNHTVVLYDNIATYQHYKYLGCATSSCHHRDTDNTAWVDQFRLRVQHQKHFGCYIHATHEDEALLFKFYSTNTVFHAVFWP 178
BLAST of SMED30030536 vs. Planmine SMEST
Match: SMESG000020744.1 (SMESG000020744.1) HSP 1 Score: 225.328 bits (573), Expect = 5.463e-76 Identity = 111/111 (100.00%), Postives = 111/111 (100.00%), Query Frame = 2 Query: 50 MFSQTEAIYRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFITNKDNQTAILYETIYTFLQYKLFQ 382 MFSQTEAIYRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFITNKDNQTAILYETIYTFLQYKLFQ Sbjct: 1 MFSQTEAIYRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFITNKDNQTAILYETIYTFLQYKLFQ 111
BLAST of SMED30030536 vs. Planmine SMEST
Match: SMESG000053268.1 (SMESG000053268.1) HSP 1 Score: 80.1073 bits (196), Expect = 3.958e-18 Identity = 54/161 (33.54%), Postives = 85/161 (52.80%), Query Frame = 2 Query: 131 GLIVECIAAYLYVVPYVNELNFRNSSCQFLNLS----LASSAYRTCENRNTKDVIRFPCVTIFIT-------NKDNQTAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP-GIFMI 577 G I+ECI +L + PY+ E F ++C + N S L S CEN+ TKD +FPCV + + + Q LY+ T+ +YK +CST PC + N W+ + L ++ F+CYYN ++ LL+KL ++L ++WP GIF++ Sbjct: 33 GFILECILLHLMLTPYLAEQEFEQTTCVYRNSSSRFILLSDLQLRCENKCTKDTGKFPCVWLLVNYVIPNSGQRQLQDGYLYDYYRTYNRYKKQRCSTSPCHLLLSDNIDWVISFDRTLYSKHNFSCYYNTKDHMSVLLYKLYPKSLILHCILWPFGIFIV 193
BLAST of SMED30030536 vs. Planmine SMEST
Match: SMESG000047387.1 (SMESG000047387.1) HSP 1 Score: 75.0998 bits (183), Expect = 1.873e-16 Identity = 54/169 (31.95%), Postives = 89/169 (52.66%), Query Frame = 2 Query: 110 CLSGAFVGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPC--VTIFITNKDNQ-TAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWP-GIFMIFTLISSVLF 604 C + + LIV I +L V+P+ +E +F +S+C +N ++ +S CEN+ +KD FPC +T T+ D + A +Y+ I T Y+ QC+ PC N + + +R+ K F CY N + E LL+K S+ + + WP G+ +I LI +VL+ Sbjct: 20 CSTLSLCALIVGAILIHLVVIPFTHENSFYSSTCTLINTNITTSI--KCENKCSKDRSMFPCLQITAKYTDLDQEFLAYVYDHIATLSHYRKSQCAISPCFSSTKDNGIIVSEFQSRMRKTPTFACYINAEHRDEVLLYKFYSIGMFWHCVAWPSGLLVISLLILAVLY 186
BLAST of SMED30030536 vs. Planmine SMEST
Match: SMESG000017226.1 (SMESG000017226.1) HSP 1 Score: 75.8702 bits (185), Expect = 1.955e-16 Identity = 50/170 (29.41%), Postives = 87/170 (51.18%), Query Frame = 2 Query: 137 IVECIAAYLYVVPYVNELNFRNSSCQFLNLSLASSAYRTCENRNTKDVIRFPCVTIFITNKDNQ---TAILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWPGIFMIFTLISSVLFFVYLQCIINRD 637 IV CI +L ++PY++E +F ++ C LN + R CEN+ +KD +F C+ I + + N +A LYE + T + C+T PC EE+ N + + + +Q F C+ +I + SEALL+K L +WP + ++ + S + + +C + D Sbjct: 55 IVVCILMHLVIIPYLHETSFISAQCN-LNETRMGDEVR-CENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHD 222
BLAST of SMED30030536 vs. Planmine SMEST
Match: SMESG000032303.1 (SMESG000032303.1) HSP 1 Score: 73.9442 bits (180), Expect = 5.982e-16 Identity = 55/178 (30.90%), Postives = 93/178 (52.25%), Query Frame = 2 Query: 128 VGLIVECIAAYLYVVPYVNELNFRNSSCQFLNLS-LASSAYRTCENRNTKDVIRFPCVTIFITNKDNQT-----AILYETIYTFLQYKLFQCSTVPCSVEEVYNQVWIQNLAARLSKQNKFTCYYNIYNNSEAXXXXXXXXXXXXXXXIWPGIFMIFTLISSVLFFVYLQC-IINRDK 640 GLI+ECI YL V+PY++E + N++C + S + + CEN+ TK+ F C+ I ++ K + L++ + TF ++K+ +CST+PC N+ W L ++ F C+Y + LL+KL S I++ ++WP + LIS L + C I ++DK Sbjct: 29 AGLIIECILLYLVVIPYLSEQKYINTTCHYTEGSKIGKMILKKCENKCTKETSFFYCLFIKVSYKKIHSKQLTIGYLFDNLRTFSKHKVDKCSTMPCLGLLEDNRRWAMAFNESLYRRTYFQCFYKDDETTNVLLYKLQSPTIVMNCIVWPCTLLTLGLISLFLTWYCAGCKIWSQDK 206 The following BLAST results are available for this feature:
BLAST of SMED30030536 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030536 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030536 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30030536 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030536 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030536 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030536 ID=SMED30030536|Name=SMED30030536|organism=Schmidtea mediterranea sexual|type=transcript|length=695bpback to top protein sequence of SMED30030536-orf-1 >SMED30030536-orf-1 ID=SMED30030536-orf-1|Name=SMED30030536-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=198bp MFSQTEAIYRKTRLKICCSICLSGAFVGLIVECIAAYLYVVPYVNELNFRback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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