SMED30030521

Overview
NameSMED30030521
Smed IDSMED30030521
Length (bp)585
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30030521 (SMED30030521) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30030521 (SMED30030521) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30030521 (SMED30030521) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30030521 (SMED30030521) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030521

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30030521SMESG000076527.1 SMESG000069759.1 SMESG000057276.1 SMESG000045286.1 SMESG000040833.1 SMESG000017867.1 dd_Smed_v4_12301_1_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030521SMESG000076527.1 SMESG000069759.1 SMESG000057276.1 SMESG000045286.1 SMESG000040833.1 SMESG000017867.1 dd_Smed_v4_12301_1_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30030521SMESG000076527.1 SMESG000069759.1 SMESG000057276.1 SMESG000045286.1 SMESG000040833.1 SMESG000017867.1 dd_Smed_v4_12301_1_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30030521 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005508:44380..45060 +2459
v31.007618:17710..18301 +2368
v31.000012:268398..268974 +2250
v31.014051:477..1038 +1607
v31.038415:2178..2763 -1220
v31.023641:13394..13999 +1205
v31.023641:13451..14024 +1163
Homology
[Close]
BLAST of SMED30030521 vs. TrEMBL
Match: A0A0X9SRF7 (Putative BRCA2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.934e-24
Identity = 52/104 (50.00%), Postives = 76/104 (73.08%), Query Frame = -2 
Query:  252 LFNFNIRKYDGRSIKETSNWIKDIEEWIRMNNLCIISVVDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEYERFATFEIRTR 563
            + NFNIRK++GR+I E    +KDI++W+ + +L +I + DLLLS+EA + W DFKT  +T E+A+SWF+DTFT +KSI + I +L  + QK  E FATFE+R +
Sbjct:  347 ILNFNIRKFNGRNIFEAEQLLKDIDDWLGIRDLSLIGIFDLLLSEEAKIPWKDFKTIGLTDEDARSWFVDTFTSQKSIADKIYDLAHVKQKN-EGFATFELRVK 449
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BLAST of SMED30030521 vs. Planmine SMEST
Match: SMESG000030738.1 (SMESG000030738.1)

HSP 1 Score: 317.39 bits (812), Expect = 3.932e-109
Identity = 170/193 (88.08%), Postives = 173/193 (89.64%), Query Frame = -2 
Query:    6 LPKKAQDLFNFNIRKYDGRSIKETSNWIKDIEEWIRMNNLCIISVVDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEYERFATFEIRTRNLLESIFAPGXXXXXXXXXXISK*ARSTALKEKIISKPVMKVEDIRKLVKLCKSQEYSQHSSSEIFVDTVQKKSYSDALKQN 584
            L KKAQDLFNFNIRKYDGRSI+E SNWIKDIEEWIRMNNLCIISV DLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVE  EINQK  ERFATFEIR RNLLESIF  GI REEIV  IISK ARSTALKEKIISKPVMKVEDIR L KL ++QEY QHSSSEIFVD VQKKSYSD LKQN
Sbjct:   20 LLKKAQDLFNFNIRKYDGRSIEEASNWIKDIEEWIRMNNLCIISVFDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVEFAEINQKGDERFATFEIRVRNLLESIFDSGISREEIVKDIISKRARSTALKEKIISKPVMKVEDIRNLAKLYETQEYVQHSSSEIFVDAVQKKSYSDVLKQN 212
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BLAST of SMED30030521 vs. Planmine SMEST
Match: SMESG000047654.1 (SMESG000047654.1)

HSP 1 Score: 287.345 bits (734), Expect = 2.377e-98
Identity = 149/193 (77.20%), Postives = 152/193 (78.76%), Query Frame = -2 
Query:    6 LPKKAQDLFNFNIRKYDGRSIKETSNWIKDIEEWIRMNNLCIISVVDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEYERFATFEIRTRNLLESIFAPGXXXXXXXXXXISK*ARSTALKEKIISKPVMKVEDIRKLVKLCKSQEYSQHSSSEIFVDTVQKKSYSDALKQN 584
            LPKKAQDLFNFNIRKYDGRSI + SNWIKDIEEWIRMNNLCIISV DLLL DEAGVLWHDFKTDAVT EEAKSWFLDTFTIKKSITNMIVEL E+NQK  ERFATFEIR                          ARSTALKEKIISKP MKVEDI  L KL +SQEY QHSSSEIFVD VQKKSYSDALKQN
Sbjct:   31 LPKKAQDLFNFNIRKYDGRSIDDASNWIKDIEEWIRMNNLCIISVFDLLLFDEAGVLWHDFKTDAVTNEEAKSWFLDTFTIKKSITNMIVELAELNQKGDERFATFEIRR-------------------------ARSTALKEKIISKPFMKVEDIHNLAKLNESQEYVQHSSSEIFVDAVQKKSYSDALKQN 198
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BLAST of SMED30030521 vs. Planmine SMEST
Match: SMESG000080880.1 (SMESG000080880.1)

HSP 1 Score: 230.335 bits (586), Expect = 2.981e-74
Identity = 129/188 (68.62%), Postives = 142/188 (75.53%), Query Frame = -2 
Query:   12 KAQDLFNFNIRKYDGRSIKETSNWIKDIEEWIRMNNLCIISVVDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEYERFATFEIRTRNLLESIFAPGXXXXXXXXXXISK*ARSTALKEKIISKPVMKVEDIRKLVKLCKSQEYSQHSSSEIFVDTVQKKSYSDALK 575
            KA DLFNFNIR++DGRSI+E  NWIKDIEEWIRM+NLCIISV DLLLSDEA VL H FKT+AVTKEEAKS FLDTF +KKSITNMI+EL EINQK  ERFATFEIR RNLLES+F  GI               S  + + IISK   K  DIR L KL +S EY+QHSSSEIFVD VQKK YS+ LK
Sbjct:   39 KALDLFNFNIREHDGRSIEEALNWIKDIEEWIRMHNLCIISVFDLLLSDEALVLLHGFKTEAVTKEEAKSMFLDTFIVKKSITNMILELAEINQKGDERFATFEIRVRNLLESVFDSGIS--------------SGKIVKDIISKRARK--DIRNLAKLYES-EYAQHSSSEIFVDAVQKKRYSNELK 209
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BLAST of SMED30030521 vs. Planmine SMEST
Match: SMESG000017867.1 (SMESG000017867.1)

HSP 1 Score: 212.231 bits (539), Expect = 7.985e-71
Identity = 114/134 (85.07%), Postives = 117/134 (87.31%), Query Frame = -2 
Query:  168 QDLFNFNIRKYDGRSIKETSNWIKDIEEWIRMNNLCIISVVDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEYERFATFEIRTRNLLESIFAPGXXXXXXXXXXISK*ARST 569
            +DLFNFNIRKYDGRSI+E SNWIKDIEEWIRM NLCIISV DLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVE  EINQK  ERFATFEIR RNLL SIF  GI REEI   IISK AR +
Sbjct:   14 KDLFNFNIRKYDGRSIEEASNWIKDIEEWIRMKNLCIISVFDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVEFAEINQKGDERFATFEIRVRNLLGSIFDSGISREEIENDIISKQARKS 147
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BLAST of SMED30030521 vs. Planmine SMEST
Match: SMESG000053029.1 (SMESG000053029.1)

HSP 1 Score: 213.386 bits (542), Expect = 2.178e-70
Identity = 112/157 (71.34%), Postives = 121/157 (77.07%), Query Frame = -2 
Query:    6 MNNLCIISVVDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEYERFATFEIRTRNLLESIFAPGXXXXXXXXXXISK*ARSTALKEKIISKPVMKVEDIRKLVKLCKSQEYSQHSSSEIFVDTVQKKSYSDALKQN 476
            MNNLCIISV DLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNM VEL +INQK  ERFA FEI    +++ I               SK ARSTALKE  ISKPVMK+E+IR L KL +SQ+Y QHSSSE+FVD VQKKSYS A KQN
Sbjct:    1 MNNLCIISVFDLLLSDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMFVELAKINQKGDERFAAFEINREEIVKDIL--------------SKRARSTALKENKISKPVMKIENIRNLAKLYESQDYLQHSSSEVFVDAVQKKSYSYAFKQN 143
The following BLAST results are available for this feature:
BLAST of SMED30030521 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A0X9SRF71.934e-2450.00Putative BRCA2 OS=Schmidtea mediterranea OX=79327 ... [more]
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BLAST of SMED30030521 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030521 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000030738.13.932e-10988.08SMESG000030738.1[more]
SMESG000047654.12.377e-9877.20SMESG000047654.1[more]
SMESG000080880.12.981e-7468.62SMESG000080880.1[more]
SMESG000017867.17.985e-7185.07SMESG000017867.1[more]
SMESG000053029.12.178e-7071.34SMESG000053029.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030521 ID=SMED30030521|Name=SMED30030521|organism=Schmidtea mediterranea sexual|type=transcript|length=585bp
TAAATATTCTGTTTCAAAGCATCCGAGTAACTCTTCTTTTGTACAGTATC
CACAAATATCTCTGAAGAAGAATGTTGTGAATATTCTTGACTTTTACATA
ATTTGACCAACTTGCGAATATCTTCTACTTTCATAACTGGTTTAGAAATT
ATTTTTTCTTTAAGCGCCGTACTTCTAGCTCATTTAGAAATAATAACTTA
TACAATTTCTTCACGAATAATTCCTGGAGCAAAAATACTTTCCAAAAGAT
TTCTTGTCCTGATTTCAAATGTAGCAAATCTTTCATATTCTTTTTGATTA
ATTTCGGGCAATTCTACAATCATATTGGTAATTGATTTCTTTATTGTAAA
TGTATCTAAAAACCATGATTTTGCTTCCTCCTTTGTAACTGCATCTGTTT
TGAAATCATGCCATAAGACTCCAGCTTCATCAGACAGTAATAAATCTACA
ACACTAATGATGCACAAATTGTTCATTCGGATCCATTCTTCGATATCTTT
AATCCAATTTGATGTTTCTTTGATACTTCGACCATCATATTTTCGGATAT
TGAAATTGAATAAATCCTGCGCTTTTTTCGGTAAT
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protein sequence of SMED30030521-orf-1

>SMED30030521-orf-1 ID=SMED30030521-orf-1|Name=SMED30030521-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp
LPKKAQDLFNFNIRKYDGRSIKETSNWIKDIEEWIRMNNLCIISVVDLLL
SDEAGVLWHDFKTDAVTKEEAKSWFLDTFTIKKSITNMIVELPEINQKEY
ERFATFEIRTRNLLESIFAPGIIREEIV*
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