Myelin expression factor 2 isoform 2

Overview
NameMyelin expression factor 2 isoform 2
Smed IDSMED30030516
Length (bp)1770
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Myelin expression factor 2 isoform 2 (SMED30030516) t-SNE clustered cells

Violin plots show distribution of expression levels for Myelin expression factor 2 isoform 2 (SMED30030516) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myelin expression factor 2 isoform 2 (SMED30030516) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myelin expression factor 2 isoform 2 (SMED30030516) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030516

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30030516h1SMcG0008246 Contig6834uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30030516h1SMcG0008246 Contig6834newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
X1 cellSMED30030516h1SMcG0008246 SmedASXL_002156SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30030516h1SMcG0008246 SmedASXL_002156SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30030516h1SMcG0008246 dd_Smed_v4_2707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30030516h1SMcG0008246 dd_Smed_v4_2707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30030516h1SMcG0008246 dd_Smed_v4_2707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030516h1SMcG0008246 dd_Smed_v4_2707_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Human
Match: HNRNPM (heterogeneous nuclear ribonucleoprotein M [Source:HGNC Symbol;Acc:HGNC:5046])

HSP 1 Score: 127.102 bits (318), Expect = 1.447e-31
Identity = 69/195 (35.38%), Postives = 114/195 (58.46%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ITNIPF+   + LKD+ K+++G    ++ + D  GK  G AV+EFK  +  +KA + ++K+S++GR + V+E+ + +  R ++                        +K G + +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV AI +FN +++ +R + ++MD+   P
Sbjct:   75 ITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAM------------------------QKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALP 245          

HSP 2 Score: 62.3882 bits (150), Expect = 9.124e-10
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 3
Query:  459 FIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            FI N+ + + WQ LKD+ K   G V +V+L  DA+GKSRG   VEF+  E    A  + N   +S R +K++ D  G+ A
Sbjct:   74 FITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA 153          

HSP 3 Score: 57.3806 bits (137), Expect = 3.267e-8
Identity = 25/75 (33.33%), Postives = 43/75 (57.33%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            ++ + N+ +   WK LK  FS A  ++  +I  D   +S+GIG V FE   +AV+  +M +G L   RP+H++ +
Sbjct:  166 TVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMD 240          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Human
Match: HNRNPM (heterogeneous nuclear ribonucleoprotein M [Source:HGNC Symbol;Acc:HGNC:5046])

HSP 1 Score: 123.635 bits (309), Expect = 2.640e-30
Identity = 74/211 (35.07%), Postives = 121/211 (57.35%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQ----------PQGSLFN------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ITNIPF+   + LKD+ K+++G    ++ + D  GK  G AV+EFK  +  +KA + ++K+S++GR + V+E+ + +  R ++          G           P   L N      +I+ L+  G + +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV AI +FN +++ +R + ++MD+   P
Sbjct:   75 ITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQA-GRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALP 284          

HSP 2 Score: 62.3882 bits (150), Expect = 7.908e-10
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 3
Query:  459 FIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            FI N+ + + WQ LKD+ K   G V +V+L  DA+GKSRG   VEF+  E    A  + N   +S R +K++ D  G+ A
Sbjct:   74 FITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA 153          

HSP 3 Score: 60.4622 bits (145), Expect = 3.270e-9
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 3
Query: 1338 NPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NP+ PN    +  A +   ++ + N+ +   WK LK  FS A  ++  +I  D   +S+GIG V FE   +AV+  +M +G L   RP+H++ +
Sbjct:  186 NPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMD 279          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Human
Match: MYEF2 (myelin expression factor 2 [Source:HGNC Symbol;Acc:HGNC:17940])

HSP 1 Score: 125.946 bits (315), Expect = 5.989e-30
Identity = 73/211 (34.60%), Postives = 123/211 (58.29%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQP-QGS-LFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F     P  GS L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:  104 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGGSFPGGHVPDMGSGLMNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 313          

HSP 2 Score: 76.6406 bits (187), Expect = 5.584e-14
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            E+ G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV F++PE A  A  + N   +S REI +R+D+
Sbjct:  492 ERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLDR 574          

HSP 3 Score: 69.3218 bits (168), Expect = 9.788e-12
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   93 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 182          

HSP 4 Score: 56.6102 bits (135), Expect = 9.634e-8
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRF++   A +   +M+G    GR I +  +
Sbjct:  501 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLD 573          

HSP 5 Score: 53.5286 bits (127), Expect = 8.164e-7
Identity = 32/118 (27.12%), Postives = 57/118 (48.31%), Query Frame = 3
Query: 1260 GVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            G++N+  S++N  N  P   I+ L            +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  204 GLMNLPPSILNNPNIPPEV-ISNLQ-----------AGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 309          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Human
Match: MYEF2 (myelin expression factor 2 [Source:HGNC Symbol;Acc:HGNC:17940])

HSP 1 Score: 125.946 bits (315), Expect = 6.914e-30
Identity = 73/211 (34.60%), Postives = 123/211 (58.29%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQP-QGS-LFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F     P  GS L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:  104 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGGSFPGGHVPDMGSGLMNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 313          

HSP 2 Score: 76.6406 bits (187), Expect = 5.579e-14
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            E+ G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV F++PE A  A  + N   +S REI +R+D+
Sbjct:  516 ERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLDR 598          

HSP 3 Score: 69.3218 bits (168), Expect = 1.106e-11
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   93 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 182          

HSP 4 Score: 56.6102 bits (135), Expect = 1.021e-7
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRF++   A +   +M+G    GR I +  +
Sbjct:  525 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLD 597          

HSP 5 Score: 53.5286 bits (127), Expect = 8.560e-7
Identity = 32/118 (27.12%), Postives = 57/118 (48.31%), Query Frame = 3
Query: 1260 GVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            G++N+  S++N  N  P   I+ L            +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  204 GLMNLPPSILNNPNIPPEV-ISNLQ-----------AGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 309          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Human
Match: HNRNPM (heterogeneous nuclear ribonucleoprotein M [Source:HGNC Symbol;Acc:HGNC:5046])

HSP 1 Score: 126.331 bits (316), Expect = 8.566e-30
Identity = 69/195 (35.38%), Postives = 114/195 (58.46%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ITNIPF+   + LKD+ K+++G    ++ + D  GK  G AV+EFK  +  +KA + ++K+S++GR + V+E+ + +  R ++                        +K G + +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV AI +FN +++ +R + ++MD+   P
Sbjct:   75 ITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAM------------------------QKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALP 245          

HSP 2 Score: 69.3218 bits (168), Expect = 1.013e-11
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F   G V + D+K + +GKS+G G V+FE+PE A  A  + N   +S REI +R+D+
Sbjct:  616 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKME-NGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDR 689          

HSP 3 Score: 61.6178 bits (148), Expect = 3.055e-9
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 3
Query:  459 FIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            FI N+ + + WQ LKD+ K   G V +V+L  DA+GKSRG   VEF+  E    A  + N   +S R +K++ D  G+ A
Sbjct:   74 FITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA 153          

HSP 4 Score: 56.9954 bits (136), Expect = 7.570e-8
Identity = 25/75 (33.33%), Postives = 43/75 (57.33%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            ++ + N+ +   WK LK  FS A  ++  +I  D   +S+GIG V FE   +AV+  +M +G L   RP+H++ +
Sbjct:  166 TVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMD 240          

HSP 5 Score: 56.225 bits (134), Expect = 1.535e-7
Identity = 25/74 (33.78%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF +TWK LK  F++   +++ +I  ++  +SKG G+V+FE+   A R   MM+G    GR I +  +
Sbjct:  616 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKMEN-GKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRID 688          

HSP 6 Score: 50.8322 bits (120), Expect = 6.617e-6
Identity = 29/77 (37.66%), Postives = 47/77 (61.04%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F +    CG + Y D   +NGK  G  V++F++ + A++A + M+   ++GREI VR + N
Sbjct:  618 VRNLPFDFTWKMLKDKFNE----CGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRN 690          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Celegans
Match: sup-46 (SUPpressor [Source:UniProtKB/TrEMBL;Acc:Q9XVS2])

HSP 1 Score: 95.1301 bits (235), Expect = 1.287e-20
Identity = 65/221 (29.41%), Postives = 111/221 (50.23%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLG---ICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVRE-----------ETNKDRERLSLSTPQHNFSSAGQPQG----------SLFNLINELEK----DGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGK 689
            ITNI +     +LK + +++ G    C  L+  D+NGK  GNAV+EF+T +GA+K V+ + K+   GR II +E           +T    + LS +           P+            LF L  E  +    + P+   +FIANL + +   KL ++F  AG++  +D + D +GKS+G   +++  P EAV A+ + N + + +R + ++MD+  K
Sbjct:   49 ITNIAYEARWVDLKSLVREKGGEVAFCELLE--DRNGKPKGNAVVEFETREGAEKCVENLQKFDWKGRTIIAKEIRDPNAFFRTIKTETGIDYLSRTGGGGAGGRGPPPKDLDRPTRTGTYDLFGLNMEFLRQHNIEPPLCERIFIANLAFNVGTDKLYEVFGMAGKITWMDFRIDKEGKSKGVCVIQYTHPIEAVQAVSMLNGQRLFDRNLVVKMDRFDK 267          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Celegans
Match: sup-46 (SUPpressor [Source:UniProtKB/TrEMBL;Acc:Q9XVS2])

HSP 1 Score: 95.1301 bits (235), Expect = 1.287e-20
Identity = 65/221 (29.41%), Postives = 111/221 (50.23%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLG---ICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVRE-----------ETNKDRERLSLSTPQHNFSSAGQPQG----------SLFNLINELEK----DGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGK 689
            ITNI +     +LK + +++ G    C  L+  D+NGK  GNAV+EF+T +GA+K V+ + K+   GR II +E           +T    + LS +           P+            LF L  E  +    + P+   +FIANL + +   KL ++F  AG++  +D + D +GKS+G   +++  P EAV A+ + N + + +R + ++MD+  K
Sbjct:   49 ITNIAYEARWVDLKSLVREKGGEVAFCELLE--DRNGKPKGNAVVEFETREGAEKCVENLQKFDWKGRTIIAKEIRDPNAFFRTIKTETGIDYLSRTGGGGAGGRGPPPKDLDRPTRTGTYDLFGLNMEFLRQHNIEPPLCERIFIANLAFNVGTDKLYEVFGMAGKITWMDFRIDKEGKSKGVCVIQYTHPIEAVQAVSMLNGQRLFDRNLVVKMDRFDK 267          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797])

HSP 1 Score: 67.781 bits (164), Expect = 1.325e-11
Identity = 48/178 (26.97%), Postives = 80/178 (44.94%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREI---IVREETNKDRE-RLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            KE  +    + G     + +   GK  G   + F   + A+ AV+ +H  +I G ++   + R +   +R   L     QH      + QG                 +L++ NLD T+    LK  F++ G +    + TD +G+S+GFG V FE PEEA  A+   N+KMV ++ +
Sbjct:  166 KETLEKLFAKFGNITSCEVMTVEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGV----------------NLYVKNLDETVDDDGLKKQFESYGNITSAKVMTDENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPL 327          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797])

HSP 1 Score: 67.781 bits (164), Expect = 1.325e-11
Identity = 48/178 (26.97%), Postives = 80/178 (44.94%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREI---IVREETNKDRE-RLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            KE  +    + G     + +   GK  G   + F   + A+ AV+ +H  +I G ++   + R +   +R   L     QH      + QG                 +L++ NLD T+    LK  F++ G +    + TD +G+S+GFG V FE PEEA  A+   N+KMV ++ +
Sbjct:  166 KETLEKLFAKFGNITSCEVMTVEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGV----------------NLYVKNLDETVDDDGLKKQFESYGNITSAKVMTDENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPL 327          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Celegans
Match: pab-1 (Polyadenylate-binding protein [Source:UniProtKB/TrEMBL;Acc:Q7K797])

HSP 1 Score: 67.781 bits (164), Expect = 1.325e-11
Identity = 48/178 (26.97%), Postives = 80/178 (44.94%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREI---IVREETNKDRE-RLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            KE  +    + G     + +   GK  G   + F   + A+ AV+ +H  +I G ++   + R +   +R   L     QH      + QG                 +L++ NLD T+    LK  F++ G +    + TD +G+S+GFG V FE PEEA  A+   N+KMV ++ +
Sbjct:  166 KETLEKLFAKFGNITSCEVMTVEGKSKGFGFVAFANPEEAETAVQALHDSTIEGTDLKLHVCRAQKKSERHAELKKKHEQHKAERMQKYQGV----------------NLYVKNLDETVDDDGLKKQFESYGNITSAKVMTDENGRSKGFGFVCFEKPEEATSAVTEMNSKMVCSKPL 327          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Fly
Match: rump (gene:FBgn0267790 transcript:FBtr0082123)

HSP 1 Score: 97.8265 bits (242), Expect = 5.731e-21
Identity = 74/254 (29.13%), Postives = 121/254 (47.64%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI----DKNGKFSGNAVMEFKTVDGAQKA---------------VKEMHKYSINGREIIVREE------------------------------------TNKDRERLSLSTPQHNFS-----SAGQPQGSLFNL----INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            I+NIP++   ++LKD+F+    I G ++Y+    D++GK  G  ++EFK  +  QKA               VKE H    +    IVR+                                     + +D +RLS    ++NF+            +L+ L    +  L   GP+ N +F+ANLDY +  +KLK +FK AG+V+ VDL  D +G SRGF  +E++ P EAV AI + + +M+ +R + +R+D+
Sbjct:   61 ISNIPYDYRWQDLKDLFRR---IVGSIEYVQLFFDESGKARGCGIVEFKDPENVQKALEKMNRYEVNGRELVVKEDHGEQRDQYGRIVRDGGGGGGGGGGVQGGNGGNNGGGGGGGRDHMDDRDRGFSRRDDDRLS---GRNNFNMMSNDYNNSSNYNLYGLSASFLESLGISGPLHNKVFVANLDYKVDNKKLKQVFKLAGKVQSVDLSLDKEGNSRGFAVIEYDHPVEAVQAISMLDRQMLFDRRMTVRLDR 308          

HSP 2 Score: 60.077 bits (144), Expect = 3.699e-9
Identity = 29/75 (38.67%), Postives = 44/75 (58.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMD 677
            ++I+N+ Y   WQ LKD+F+   G + +V L  D  GK+RG G VEF+ PE    A+   N   V+ RE+ ++ D
Sbjct:   59 VYISNIPYDYRWQDLKDLFRRIVGSIEYVQLFFDESGKARGCGIVEFKDPENVQKALEKMNRYEVNGRELVVKED 133          

HSP 3 Score: 52.373 bits (124), Expect = 9.797e-7
Identity = 25/76 (32.89%), Postives = 40/76 (52.63%), Query Frame = 3
Query: 1380 SKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEF 1607
            S +++IKN+P + TW+ L+  F +   +   EI      R   +G+VRF    DA     +MDG+  DGR I + +
Sbjct:  562 SDTIIIKNVPITCTWQTLRDKFREIGDVKFAEI------RGNDVGVVRFFKERDAELAIALMDGSRLDGRNIKVTY 631          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Fly
Match: rump (gene:FBgn0267790 transcript:FBtr0304684)

HSP 1 Score: 65.855 bits (159), Expect = 5.700e-12
Identity = 30/82 (36.59%), Postives = 57/82 (69.51%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI----DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRE 344
            I+NIP++   ++LKD+F+    I G ++Y+    D++GK  G  ++EFK  +  QKA+++M++Y +NGRE++V+E+  + R+
Sbjct:   61 ISNIPYDYRWQDLKDLFR---RIVGSIEYVQLFFDESGKARGCGIVEFKDPENVQKALEKMNRYEVNGRELVVKEDHGEQRD 139          

HSP 2 Score: 58.5362 bits (140), Expect = 2.116e-9
Identity = 29/75 (38.67%), Postives = 44/75 (58.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMD 677
            ++I+N+ Y   WQ LKD+F+   G + +V L  D  GK+RG G VEF+ PE    A+   N   V+ RE+ ++ D
Sbjct:   59 VYISNIPYDYRWQDLKDLFRRIVGSIEYVQLFFDESGKARGCGIVEFKDPENVQKALEKMNRYEVNGRELVVKED 133          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Fly
Match: pAbp (gene:FBgn0265297 transcript:FBtr0086738)

HSP 1 Score: 53.1434 bits (126), Expect = 5.698e-7
Identity = 40/168 (23.81%), Postives = 72/168 (42.86%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREET-NKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAK 641
            K + D F     I       D+ G   G   + F+T + A  ++ +++   +NG+++ V +    K+RE+                         EL +   +  ++++ N       +KLK+ F+  G++    + +  DGKS+GFG V FET E A  A+   N K
Sbjct:  104 KAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREK-------------------------ELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGK 246          

HSP 2 Score: 50.8322 bits (120), Expect = 3.337e-6
Identity = 40/185 (21.62%), Postives = 82/185 (44.32%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREI-------IVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            ++LK+ F+    I        ++GK  G   + F+T + A+ AV+     ++NG+++       + R +   +R+               Q     F  + +   +     +L++ NLD T+   +L+  F   G +    + TD +G+S+GFG V F    EA  A+   N ++V ++ + + +
Sbjct:  197 EKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQ-----ALNGKDMGEGKSLYVARAQKKAERQ---------------QELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVAL 361          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Fly
Match: pAbp (gene:FBgn0265297 transcript:FBtr0086736)

HSP 1 Score: 53.1434 bits (126), Expect = 5.698e-7
Identity = 40/168 (23.81%), Postives = 72/168 (42.86%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREET-NKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAK 641
            K + D F     I       D+ G   G   + F+T + A  ++ +++   +NG+++ V +    K+RE+                         EL +   +  ++++ N       +KLK+ F+  G++    + +  DGKS+GFG V FET E A  A+   N K
Sbjct:  104 KAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREK-------------------------ELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGK 246          

HSP 2 Score: 50.8322 bits (120), Expect = 3.337e-6
Identity = 40/185 (21.62%), Postives = 82/185 (44.32%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREI-------IVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            ++LK+ F+    I        ++GK  G   + F+T + A+ AV+     ++NG+++       + R +   +R+               Q     F  + +   +     +L++ NLD T+   +L+  F   G +    + TD +G+S+GFG V F    EA  A+   N ++V ++ + + +
Sbjct:  197 EKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQ-----ALNGKDMGEGKSLYVARAQKKAERQ---------------QELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVAL 361          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Fly
Match: pAbp (gene:FBgn0265297 transcript:FBtr0086740)

HSP 1 Score: 53.1434 bits (126), Expect = 5.698e-7
Identity = 40/168 (23.81%), Postives = 72/168 (42.86%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREET-NKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAK 641
            K + D F     I       D+ G   G   + F+T + A  ++ +++   +NG+++ V +    K+RE+                         EL +   +  ++++ N       +KLK+ F+  G++    + +  DGKS+GFG V FET E A  A+   N K
Sbjct:  104 KAIYDTFSAFGNILSCKVATDEKGNSKGYGFVHFETEEAANTSIDKVNGMLLNGKKVYVGKFIPRKEREK-------------------------ELGEKAKLFTNVYVKNFTEDFDDEKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQALNGK 246          

HSP 2 Score: 50.8322 bits (120), Expect = 3.337e-6
Identity = 40/185 (21.62%), Postives = 82/185 (44.32%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREI-------IVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            ++LK+ F+    I        ++GK  G   + F+T + A+ AV+     ++NG+++       + R +   +R+               Q     F  + +   +     +L++ NLD T+   +L+  F   G +    + TD +G+S+GFG V F    EA  A+   N ++V ++ + + +
Sbjct:  197 EKLKEFFEPYGKITSYKVMSKEDGKSKGFGFVAFETTEAAEAAVQ-----ALNGKDMGEGKSLYVARAQKKAERQ---------------QELKRKFEELKQKRHESVFGVNLYVKNLDDTIDDDRLRIAFSPYGNITSAKVMTDEEGRSKGFGFVCFNAASEATCAVTELNGRVVGSKPLYVAL 361          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Zebrafish
Match: myef2 (myelin expression factor 2 [Source:ZFIN;Acc:ZDB-GENE-051120-114])

HSP 1 Score: 130.183 bits (326), Expect = 1.424e-31
Identity = 71/209 (33.97%), Postives = 119/209 (56.94%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M K+ +NGR + ++E+ + +  R  L      +        S  N+              I+ L+  G +  ++F+ANLD+ + W+KLK++F  AG VR  D+K D DGKSRG GTV FE P EAV AI +FN +M+ +R++ ++MD    P
Sbjct:   85 ISNIPYDKKWQAIKDLMREKVGEVTYVELFKDGEGKSRGCGVVEFKDEEFVKKAIEVMSKHDLNGRPLNIKEDPDGEHARRVLQRSGRMYGGGRGQDSSGINIPPSIANNPNIPHEIISALQA-GRLGATIFVANLDFKVGWKKLKEVFGMAGTVRRADVKEDKDGKSRGMGTVTFEQPLEAVQAISMFNGQMLFDRQMHVKMDDKSLP 292          

HSP 2 Score: 75.485 bits (184), Expect = 7.330e-14
Identity = 32/75 (42.67%), Postives = 52/75 (69.33%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL Y L+WQKLK+ F + G+V + ++K + +GKS+G GTV F++PE A  A  L N   ++ RE+ +R+D+
Sbjct:  478 IFVRNLSYDLSWQKLKEKFSHCGQVMYAEIKME-NGKSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRLDR 551          

HSP 3 Score: 58.9214 bits (141), Expect = 1.131e-8
Identity = 30/83 (36.14%), Postives = 48/83 (57.83%), Query Frame = 3
Query:  450 NSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            N +FI+N+ Y   WQ +KD+ +   G V +V+L  D +GKSRG G VEF+  E    AI + +   ++ R + ++ D  G+ A
Sbjct:   81 NRVFISNIPYDKKWQAIKDLMREKVGEVTYVELFKDGEGKSRGCGVVEFKDEEFVKKAIEVMSKHDLNGRPLNIKEDPDGEHA 163          

HSP 4 Score: 51.9878 bits (123), Expect = 1.459e-6
Identity = 22/74 (29.73%), Postives = 43/74 (58.11%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+ +  +W+ LK  FS    +M+ EI  ++  +SKG G VRF++   A +   +M+GT  +GR + +  +
Sbjct:  478 IFVRNLSYDLSWQKLKEKFSHCGQVMYAEIKMEN-GKSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRLD 550          

HSP 5 Score: 50.0618 bits (118), Expect = 6.512e-6
Identity = 29/106 (27.36%), Postives = 54/106 (50.94%), Query Frame = 3
Query: 1314 SGIN---QLHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            SGIN    +  NP+ P+    +  A +   ++ + N+ F   WK LK  F  A  +   ++  D   +S+G+G V FE   +AV+  +M +G +   R +H++ ++
Sbjct:  183 SGINIPPSIANNPNIPHEIISALQAGRLGATIFVANLDFKVGWKKLKEVFGMAGTVRRADVKEDKDGKSRGMGTVTFEQPLEAVQAISMFNGQMLFDRQMHVKMDD 288          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Zebrafish
Match: myef2 (myelin expression factor 2 [Source:ZFIN;Acc:ZDB-GENE-051120-114])

HSP 1 Score: 130.183 bits (326), Expect = 1.446e-31
Identity = 71/209 (33.97%), Postives = 119/209 (56.94%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M K+ +NGR + ++E+ + +  R  L      +        S  N+              I+ L+  G +  ++F+ANLD+ + W+KLK++F  AG VR  D+K D DGKSRG GTV FE P EAV AI +FN +M+ +R++ ++MD    P
Sbjct:   85 ISNIPYDKKWQAIKDLMREKVGEVTYVELFKDGEGKSRGCGVVEFKDEEFVKKAIEVMSKHDLNGRPLNIKEDPDGEHARRVLQRSGRMYGGGRGQDSSGINIPPSIANNPNIPHEIISALQA-GRLGATIFVANLDFKVGWKKLKEVFGMAGTVRRADVKEDKDGKSRGMGTVTFEQPLEAVQAISMFNGQMLFDRQMHVKMDDKSLP 292          

HSP 2 Score: 75.485 bits (184), Expect = 6.596e-14
Identity = 32/75 (42.67%), Postives = 52/75 (69.33%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL Y L+WQKLK+ F + G+V + ++K + +GKS+G GTV F++PE A  A  L N   ++ RE+ +R+D+
Sbjct:  482 IFVRNLSYDLSWQKLKEKFSHCGQVMYAEIKME-NGKSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRLDR 555          

HSP 3 Score: 58.9214 bits (141), Expect = 1.197e-8
Identity = 30/83 (36.14%), Postives = 48/83 (57.83%), Query Frame = 3
Query:  450 NSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            N +FI+N+ Y   WQ +KD+ +   G V +V+L  D +GKSRG G VEF+  E    AI + +   ++ R + ++ D  G+ A
Sbjct:   81 NRVFISNIPYDKKWQAIKDLMREKVGEVTYVELFKDGEGKSRGCGVVEFKDEEFVKKAIEVMSKHDLNGRPLNIKEDPDGEHA 163          

HSP 4 Score: 52.373 bits (124), Expect = 1.379e-6
Identity = 22/74 (29.73%), Postives = 43/74 (58.11%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+ +  +W+ LK  FS    +M+ EI  ++  +SKG G VRF++   A +   +M+GT  +GR + +  +
Sbjct:  482 IFVRNLSYDLSWQKLKEKFSHCGQVMYAEIKMEN-GKSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRLD 554          

HSP 5 Score: 50.0618 bits (118), Expect = 6.260e-6
Identity = 29/106 (27.36%), Postives = 54/106 (50.94%), Query Frame = 3
Query: 1314 SGIN---QLHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            SGIN    +  NP+ P+    +  A +   ++ + N+ F   WK LK  F  A  +   ++  D   +S+G+G V FE   +AV+  +M +G +   R +H++ ++
Sbjct:  183 SGINIPPSIANNPNIPHEIISALQAGRLGATIFVANLDFKVGWKKLKEVFGMAGTVRRADVKEDKDGKSRGMGTVTFEQPLEAVQAISMFNGQMLFDRQMHVKMDD 288          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Zebrafish
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:ZFIN;Acc:ZDB-GENE-030131-6898])

HSP 1 Score: 88.9669 bits (219), Expect = 1.481e-19
Identity = 39/85 (45.88%), Postives = 59/85 (69.41%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV F+ P EAV A+ +FN +++ NR + +++D+   P
Sbjct:   16 GKIGSTIFVANLDYKVGWKKLKEVFGMAGMVVRADILEDKDGKSRGMGTVTFDMPIEAVQAVSMFNGQLLFNRVMHVKLDEKSLP 100          

HSP 2 Score: 51.9878 bits (123), Expect = 6.250e-7
Identity = 27/94 (28.72%), Postives = 48/94 (51.06%), Query Frame = 3
Query: 1338 NPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NP+ PN       A K   ++ + N+ +   WK LK  F  A  ++  +I  D   +S+G+G V F+   +AV+  +M +G L   R +H++ +
Sbjct:    2 NPNIPNEVIHGLQAGKIGSTIFVANLDYKVGWKKLKEVFGMAGMVVRADILEDKDGKSRGMGTVTFDMPIEAVQAVSMFNGQLLFNRVMHVKLD 95          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Zebrafish
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:ZFIN;Acc:ZDB-GENE-030131-6898])

HSP 1 Score: 88.9669 bits (219), Expect = 4.929e-18
Identity = 39/85 (45.88%), Postives = 59/85 (69.41%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV F+ P EAV A+ +FN +++ NR + +++D+   P
Sbjct:  229 GKIGSTIFVANLDYKVGWKKLKEVFGMAGMVVRADILEDKDGKSRGMGTVTFDMPIEAVQAVSMFNGQLLFNRVMHVKLDEKSLP 313          

HSP 2 Score: 66.2402 bits (160), Expect = 5.911e-11
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F + G V++ D+K + +GKS+G G V F+ PE A  A    N   ++ REI +R+D+
Sbjct:  626 IFVRNLPFDFTWKMLKDTFNSCGMVQYADIKME-NGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDR 699          

HSP 3 Score: 60.4622 bits (145), Expect = 4.149e-9
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 3
Query:  453 SLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSG 686
            S+F++N+ Y + WQ LKD+ K   G V +V+   D +GKSRG   VEF T E    A+   N  +++ R +K++ D  G
Sbjct:   49 SVFVSNIPYDVKWQTLKDLMKEKVGEVTYVEHLMDGEGKSRGCAVVEFRTEELMKKAVEKVNKHVMNGRPLKVKEDPDG 127          

HSP 4 Score: 56.9954 bits (136), Expect = 5.105e-8
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F      CG + Y D   +NGK  G  V+ F   + A++A + M+ Y +NGREI VR + N
Sbjct:  628 VRNLPFDFTWKMLKDTFNS----CGMVQYADIKMENGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDRN 700          

HSP 5 Score: 55.4546 bits (132), Expect = 1.525e-7
Identity = 26/73 (35.62%), Postives = 52/73 (71.23%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREE 326
            ++NIP++   + LKD+ K+++G    ++++ D  GK  G AV+EF+T +  +KAV++++K+ +NGR + V+E+
Sbjct:   52 VSNIPYDVKWQTLKDLMKEKVGEVTYVEHLMDGEGKSRGCAVVEFRTEELMKKAVEKVNKHVMNGRPLKVKED 124          

HSP 6 Score: 52.373 bits (124), Expect = 1.533e-6
Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 3
Query: 1374 NASKS--LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NA K   + ++N+PF +TWK LK +F+    + + +I  ++  +SKG G+VRF+    A R    M+G   +GR I +  +
Sbjct:  619 NARKGCQIFVRNLPFDFTWKMLKDTFNSCGMVQYADIKMEN-GKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRID 698          

HSP 7 Score: 51.9878 bits (123), Expect = 2.054e-6
Identity = 28/97 (28.87%), Postives = 49/97 (50.52%), Query Frame = 3
Query: 1329 LHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            L  NP+ PN       A K   ++ + N+ +   WK LK  F  A  ++  +I  D   +S+G+G V F+   +AV+  +M +G L   R +H++ +
Sbjct:  212 LMNNPNIPNEVIHGLQAGKIGSTIFVANLDYKVGWKKLKEVFGMAGMVVRADILEDKDGKSRGMGTVTFDMPIEAVQAVSMFNGQLLFNRVMHVKLD 308          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Zebrafish
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:ZFIN;Acc:ZDB-GENE-030131-6898])

HSP 1 Score: 88.9669 bits (219), Expect = 4.980e-18
Identity = 39/85 (45.88%), Postives = 59/85 (69.41%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV F+ P EAV A+ +FN +++ NR + +++D+   P
Sbjct:  229 GKIGSTIFVANLDYKVGWKKLKEVFGMAGMVVRADILEDKDGKSRGMGTVTFDMPIEAVQAVSMFNGQLLFNRVMHVKLDEKSLP 313          

HSP 2 Score: 66.6254 bits (161), Expect = 5.726e-11
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F + G V++ D+K + +GKS+G G V F+ PE A  A    N   ++ REI +R+D+
Sbjct:  640 IFVRNLPFDFTWKMLKDTFNSCGMVQYADIKME-NGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDR 713          

HSP 3 Score: 60.4622 bits (145), Expect = 4.189e-9
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 3
Query:  453 SLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSG 686
            S+F++N+ Y + WQ LKD+ K   G V +V+   D +GKSRG   VEF T E    A+   N  +++ R +K++ D  G
Sbjct:   49 SVFVSNIPYDVKWQTLKDLMKEKVGEVTYVEHLMDGEGKSRGCAVVEFRTEELMKKAVEKVNKHVMNGRPLKVKEDPDG 127          

HSP 4 Score: 56.9954 bits (136), Expect = 5.062e-8
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F      CG + Y D   +NGK  G  V+ F   + A++A + M+ Y +NGREI VR + N
Sbjct:  642 VRNLPFDFTWKMLKDTFNS----CGMVQYADIKMENGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDRN 714          

HSP 5 Score: 55.4546 bits (132), Expect = 1.565e-7
Identity = 26/73 (35.62%), Postives = 52/73 (71.23%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREE 326
            ++NIP++   + LKD+ K+++G    ++++ D  GK  G AV+EF+T +  +KAV++++K+ +NGR + V+E+
Sbjct:   52 VSNIPYDVKWQTLKDLMKEKVGEVTYVEHLMDGEGKSRGCAVVEFRTEELMKKAVEKVNKHVMNGRPLKVKED 124          

HSP 6 Score: 52.373 bits (124), Expect = 1.467e-6
Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 3
Query: 1374 NASKS--LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NA K   + ++N+PF +TWK LK +F+    + + +I  ++  +SKG G+VRF+    A R    M+G   +GR I +  +
Sbjct:  633 NARKGCQIFVRNLPFDFTWKMLKDTFNSCGMVQYADIKMEN-GKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRID 712          

HSP 7 Score: 51.9878 bits (123), Expect = 2.017e-6
Identity = 28/97 (28.87%), Postives = 49/97 (50.52%), Query Frame = 3
Query: 1329 LHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            L  NP+ PN       A K   ++ + N+ +   WK LK  F  A  ++  +I  D   +S+G+G V F+   +AV+  +M +G L   R +H++ +
Sbjct:  212 LMNNPNIPNEVIHGLQAGKIGSTIFVANLDYKVGWKKLKEVFGMAGMVVRADILEDKDGKSRGMGTVTFDMPIEAVQAVSMFNGQLLFNRVMHVKLD 308          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Xenopus
Match: cep76 (centrosomal protein 76kDa [Source:Xenbase;Acc:XB-GENE-966809])

HSP 1 Score: 119.398 bits (298), Expect = 1.142e-27
Identity = 72/219 (32.88%), Postives = 121/219 (55.25%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG-----------------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ +D++G    ++ + D  GK  G+ V+EF+ ++   KAV+ M+K+ +NGR + ++ + + +  R +LS        AG                     S+ N       +I  L + G +  ++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE P EAV AI +FN + + +R + ++MD    P
Sbjct:   84 ISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRGSGVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHARRALSRGGGGGGGAGGGPFPGGHGSDMAPGMTNIPPSIVNNPNIPPEIIGAL-RAGRLGTTVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDDKSLP 301          

HSP 2 Score: 73.9442 bits (180), Expect = 3.435e-13
Identity = 34/75 (45.33%), Postives = 49/75 (65.33%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL + LTWQKLK+ F   GRV   ++K + +GKS+G GTV F+ PE A  A  L N   ++ REI +R+D+
Sbjct:  540 IFVRNLPFDLTWQKLKEKFNQCGRVMFAEIKME-NGKSKGCGTVRFDCPETAEKACRLMNGIKINGREIDVRLDR 613          

HSP 3 Score: 68.9366 bits (167), Expect = 1.106e-11
Identity = 41/128 (32.03%), Postives = 68/128 (53.12%), Query Frame = 3
Query:  315 VREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            V+EE    +E+ + +   + F   G+ +G         EK G   N +FI+N+ Y + WQ +KD+ ++  G V +V+L  DA+GKSRG G VEF   E    A+ + N   ++ R + ++ D  G+ A
Sbjct:   42 VKEEKTSLKEKTTGNRKTNRFHPYGKDKGPG-------EKKGQSRNRVFISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRGSGVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHA 162          

HSP 4 Score: 55.0694 bits (131), Expect = 3.013e-7
Identity = 25/74 (33.78%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  F+Q   +M  EI  ++  +SKG G VRF+    A +   +M+G   +GR I +  +
Sbjct:  540 IFVRNLPFDLTWQKLKEKFNQCGRVMFAEIKMEN-GKSKGCGTVRFDCPETAEKACRLMNGIKINGREIDVRLD 612          

HSP 5 Score: 50.8322 bits (120), Expect = 6.125e-6
Identity = 23/76 (30.26%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            ++ + N+ F   WK LK  F  A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  222 TVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDD 297          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Xenopus
Match: cep76 (centrosomal protein 76kDa [Source:Xenbase;Acc:XB-GENE-966809])

HSP 1 Score: 114.775 bits (286), Expect = 2.789e-26
Identity = 71/219 (32.42%), Postives = 120/219 (54.79%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG-----------------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ +D++G    ++ + D  GK   + V+EF+ ++   KAV+ M+K+ +NGR + ++ + + +  R +LS        AG                     S+ N       +I  L + G +  ++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE P EAV AI +FN + + +R + ++MD    P
Sbjct:   84 ISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSRVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHARRALSRGGGGGGGAGGGPFPGGHGSDMAPGMTNIPPSIVNNPNIPPEIIGAL-RAGRLGTTVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDDKSLP 301          

HSP 2 Score: 62.3882 bits (150), Expect = 1.570e-9
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 3
Query:  315 VREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            V+EE    +E+ + +   + F   G+ +G         EK G   N +FI+N+ Y + WQ +KD+ ++  G V +V+L  DA+GKSR    VEF   E    A+ + N   ++ R + ++ D  G+ A
Sbjct:   42 VKEEKTSLKEKTTGNRKTNRFHPYGKDKGPG-------EKKGQSRNRVFISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSRVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHA 162          

HSP 3 Score: 59.6918 bits (143), Expect = 1.084e-8
Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLF 632
            +F+ NL + LTWQKLK+ F   GRV   ++K + +GKS+G GTV F+ PE A  A   F
Sbjct:  517 IFVRNLPFDLTWQKLKEKFNQCGRVMFAEIKME-NGKSKGCGTVRFDCPETAEKACRNF 574          

HSP 4 Score: 50.8322 bits (120), Expect = 5.719e-6
Identity = 23/76 (30.26%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            ++ + N+ F   WK LK  F  A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  222 TVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDD 297          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Xenopus
Match: cep76 (centrosomal protein 76kDa [Source:Xenbase;Acc:XB-GENE-966809])

HSP 1 Score: 114.775 bits (286), Expect = 2.916e-26
Identity = 71/219 (32.42%), Postives = 120/219 (54.79%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG-----------------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ +D++G    ++ + D  GK   + V+EF+ ++   KAV+ M+K+ +NGR + ++ + + +  R +LS        AG                     S+ N       +I  L + G +  ++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE P EAV AI +FN + + +R + ++MD    P
Sbjct:   84 ISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSRVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHARRALSRGGGGGGGAGGGPFPGGHGSDMAPGMTNIPPSIVNNPNIPPEIIGAL-RAGRLGTTVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDDKSLP 301          

HSP 2 Score: 62.003 bits (149), Expect = 1.817e-9
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 3
Query:  315 VREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            V+EE    +E+ + +   + F   G+ +G         EK G   N +FI+N+ Y + WQ +KD+ ++  G V +V+L  DA+GKSR    VEF   E    A+ + N   ++ R + ++ D  G+ A
Sbjct:   42 VKEEKTSLKEKTTGNRKTNRFHPYGKDKGPG-------EKKGQSRNRVFISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSRVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHA 162          

HSP 3 Score: 59.6918 bits (143), Expect = 1.110e-8
Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLF 632
            +F+ NL + LTWQKLK+ F   GRV   ++K + +GKS+G GTV F+ PE A  A   F
Sbjct:  540 IFVRNLPFDLTWQKLKEKFNQCGRVMFAEIKME-NGKSKGCGTVRFDCPETAEKACRNF 597          

HSP 4 Score: 50.8322 bits (120), Expect = 6.129e-6
Identity = 23/76 (30.26%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            ++ + N+ F   WK LK  F  A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  222 TVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDD 297          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Xenopus
Match: cep76 (centrosomal protein 76kDa [Source:Xenbase;Acc:XB-GENE-966809])

HSP 1 Score: 112.464 bits (280), Expect = 1.351e-25
Identity = 73/223 (32.74%), Postives = 122/223 (54.71%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI----DKNGKFSGNAVM-EFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG-----------------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ +D++G   ++ Y+    D  GK   +AV+ EF+ ++   KAV+ M+K+ +NGR + ++ + + +  R +LS        AG                     S+ N       +I  L + G +  ++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE P EAV AI +FN + + +R + ++MD    P
Sbjct:   84 ISNIPYDMKWQAIKDLMRDKVG---EVTYVELFKDAEGKSRVSAVVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHARRALSRGGGGGGGAGGGPFPGGHGSDMAPGMTNIPPSIVNNPNIPPEIIGAL-RAGRLGTTVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDDKSLP 302          

HSP 2 Score: 58.5362 bits (140), Expect = 2.058e-8
Identity = 39/129 (30.23%), Postives = 66/129 (51.16%), Query Frame = 3
Query:  315 VREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFG-TVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            V+EE    +E+ + +   + F   G+ +G         EK G   N +FI+N+ Y + WQ +KD+ ++  G V +V+L  DA+GKSR     VEF   E    A+ + N   ++ R + ++ D  G+ A
Sbjct:   42 VKEEKTSLKEKTTGNRKTNRFHPYGKDKGPG-------EKKGQSRNRVFISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSAVVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHA 163          

HSP 3 Score: 50.8322 bits (120), Expect = 5.945e-6
Identity = 23/76 (30.26%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            ++ + N+ F   WK LK  F  A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  223 TVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDD 298          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Xenopus
Match: cep76 (centrosomal protein 76kDa [Source:Xenbase;Acc:XB-GENE-966809])

HSP 1 Score: 112.849 bits (281), Expect = 1.492e-25
Identity = 73/223 (32.74%), Postives = 122/223 (54.71%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI----DKNGKFSGNAVM-EFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG-----------------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ +D++G   ++ Y+    D  GK   +AV+ EF+ ++   KAV+ M+K+ +NGR + ++ + + +  R +LS        AG                     S+ N       +I  L + G +  ++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE P EAV AI +FN + + +R + ++MD    P
Sbjct:  130 ISNIPYDMKWQAIKDLMRDKVG---EVTYVELFKDAEGKSRVSAVVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHARRALSRGGGGGGGAGGGPFPGGHGSDMAPGMTNIPPSIVNNPNIPPEIIGAL-RAGRLGTTVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDDKSLP 348          

HSP 2 Score: 60.077 bits (144), Expect = 7.081e-9
Identity = 49/179 (27.37%), Postives = 83/179 (46.37%), Query Frame = 3
Query:  177 ICGKLDYIDKNGKFSGN----AVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFG-TVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            +CG+L +        GN    A     T++    A      Y ++  E  V+EE    +E+ + +   + F   G+ +G         EK G   N +FI+N+ Y + WQ +KD+ ++  G V +V+L  DA+GKSR     VEF   E    A+ + N   ++ R + ++ D  G+ A
Sbjct:   38 VCGRLLWRSGEWFLGGNRNTCAACRGGTMNNPMYAEPVGSLYLLSESEDQVKEEKTSLKEKTTGNRKTNRFHPYGKDKGPG-------EKKGQSRNRVFISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSAVVVEFRDMEYVTKAVEVMNKHDLNGRPLNIKADPDGEHA 209          

HSP 3 Score: 50.447 bits (119), Expect = 7.159e-6
Identity = 23/76 (30.26%), Postives = 41/76 (53.95%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            ++ + N+ F   WK LK  F  A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  269 TVFVANLDFKVGWKKLKEVFCIAGTVKRADIKEDKDGKSRGMGTVTFEQPIEAVQAISMFNGQFLFDRPMHVKMDD 344          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Mouse
Match: Myef2 (myelin basic protein expression factor 2, repressor [Source:MGI Symbol;Acc:MGI:104592])

HSP 1 Score: 125.946 bits (315), Expect = 1.032e-30
Identity = 72/211 (34.12%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNF--SSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F  S A      L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:   61 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 270          

HSP 2 Score: 69.3218 bits (168), Expect = 5.397e-12
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   50 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 139          

HSP 3 Score: 53.9138 bits (128), Expect = 3.793e-7
Identity = 32/110 (29.09%), Postives = 55/110 (50.00%), Query Frame = 3
Query: 1305 DPYSGINQLHP----NPSRPN---NDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            D  SG+  L P    NP+ P    ++  +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  157 DVGSGLVNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 266          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Mouse
Match: Gm9833 (predicted gene 9833 [Source:MGI Symbol;Acc:MGI:3641855])

HSP 1 Score: 126.331 bits (316), Expect = 1.678e-30
Identity = 72/211 (34.12%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNF--SSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F  S A      L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:   95 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 304          

HSP 2 Score: 69.707 bits (169), Expect = 5.116e-12
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   84 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 173          

HSP 3 Score: 53.5286 bits (127), Expect = 5.995e-7
Identity = 24/76 (31.58%), Postives = 42/76 (55.26%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  225 TIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 300          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Mouse
Match: Myef2 (myelin basic protein expression factor 2, repressor [Source:MGI Symbol;Acc:MGI:104592])

HSP 1 Score: 125.946 bits (315), Expect = 3.862e-30
Identity = 72/211 (34.12%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNF--SSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F  S A      L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:   95 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 304          

HSP 2 Score: 73.559 bits (179), Expect = 2.871e-13
Identity = 35/81 (43.21%), Postives = 50/81 (61.73%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV FE+ E A  A  + N   +S REI +R+D+
Sbjct:  469 GSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLDR 548          

HSP 3 Score: 68.9366 bits (167), Expect = 8.006e-12
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   84 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 173          

HSP 4 Score: 58.9214 bits (141), Expect = 1.292e-8
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRFE+   A +   +M+G    GR I +  +
Sbjct:  475 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLD 547          

HSP 5 Score: 53.9138 bits (128), Expect = 4.754e-7
Identity = 33/119 (27.73%), Postives = 57/119 (47.90%), Query Frame = 3
Query: 1278 SSVMNKRNDDPYSGINQLHP----NPSRPN---NDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            +S       D  SG+  L P    NP+ P    ++  +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  182 TSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 300          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Mouse
Match: Myef2 (myelin basic protein expression factor 2, repressor [Source:MGI Symbol;Acc:MGI:104592])

HSP 1 Score: 125.946 bits (315), Expect = 4.194e-30
Identity = 72/211 (34.12%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNF--SSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F  S A      L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:   95 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 304          

HSP 2 Score: 73.559 bits (179), Expect = 3.099e-13
Identity = 35/81 (43.21%), Postives = 50/81 (61.73%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV FE+ E A  A  + N   +S REI +R+D+
Sbjct:  486 GSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLDR 565          

HSP 3 Score: 68.9366 bits (167), Expect = 8.309e-12
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   84 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 173          

HSP 4 Score: 58.9214 bits (141), Expect = 1.319e-8
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRFE+   A +   +M+G    GR I +  +
Sbjct:  492 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLD 564          

HSP 5 Score: 53.9138 bits (128), Expect = 5.009e-7
Identity = 32/110 (29.09%), Postives = 55/110 (50.00%), Query Frame = 3
Query: 1305 DPYSGINQLHP----NPSRPN---NDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            D  SG+  L P    NP+ P    ++  +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  191 DVGSGLVNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 300          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Mouse
Match: Myef2 (myelin basic protein expression factor 2, repressor [Source:MGI Symbol;Acc:MGI:104592])

HSP 1 Score: 125.561 bits (314), Expect = 4.199e-30
Identity = 72/211 (34.12%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNF--SSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F  S A      L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:   51 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 260          

HSP 2 Score: 73.559 bits (179), Expect = 3.138e-13
Identity = 35/81 (43.21%), Postives = 50/81 (61.73%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV FE+ E A  A  + N   +S REI +R+D+
Sbjct:  449 GSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLDR 528          

HSP 3 Score: 68.9366 bits (167), Expect = 9.598e-12
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   40 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 129          

HSP 4 Score: 58.5362 bits (140), Expect = 1.483e-8
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRFE+   A +   +M+G    GR I +  +
Sbjct:  455 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLD 527          

HSP 5 Score: 53.9138 bits (128), Expect = 4.413e-7
Identity = 33/119 (27.73%), Postives = 57/119 (47.90%), Query Frame = 3
Query: 1278 SSVMNKRNDDPYSGINQLHP----NPSRPN---NDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            +S       D  SG+  L P    NP+ P    ++  +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  138 TSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 256          
BLAST of Myelin expression factor 2 isoform 2 vs. UniProt/SwissProt
Match: sp|Q9P2K5|MYEF2_HUMAN (Myelin expression factor 2 OS=Homo sapiens OX=9606 GN=MYEF2 PE=1 SV=3)

HSP 1 Score: 125.946 bits (315), Expect = 3.029e-29
Identity = 73/211 (34.60%), Postives = 123/211 (58.29%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQP-QGS-LFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F     P  GS L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:  104 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGGSFPGGHVPDMGSGLMNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 313          

HSP 2 Score: 76.6406 bits (187), Expect = 2.542e-13
Identity = 36/84 (42.86%), Postives = 53/84 (63.10%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            E+ G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV F++PE A  A  + N   +S REI +R+D+
Sbjct:  516 ERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLDR 598          

HSP 3 Score: 69.3218 bits (168), Expect = 5.173e-11
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   93 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 182          

HSP 4 Score: 56.6102 bits (135), Expect = 4.738e-7
Identity = 26/74 (35.14%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRF++   A +   +M+G    GR I +  +
Sbjct:  525 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLD 597          

HSP 5 Score: 53.5286 bits (127), Expect = 3.971e-6
Identity = 32/118 (27.12%), Postives = 57/118 (48.31%), Query Frame = 3
Query: 1260 GVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            G++N+  S++N  N  P   I+ L            +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  204 GLMNLPPSILNNPNIPPEV-ISNLQ-----------AGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 309          
BLAST of Myelin expression factor 2 isoform 2 vs. UniProt/SwissProt
Match: sp|Q62826|HNRPM_RAT (Heterogeneous nuclear ribonucleoprotein M OS=Rattus norvegicus OX=10116 GN=Hnrnpm PE=1 SV=4)

HSP 1 Score: 126.331 bits (316), Expect = 3.359e-29
Identity = 69/195 (35.38%), Postives = 114/195 (58.46%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ITNIPF+   + LKD+ K+++G    ++ + D  GK  G AV+EFK  +  +KA + ++K+S++GR + V+E+ + +  R ++                        +K G + +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV AI +FN +++ +R + ++MD+   P
Sbjct:   74 ITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAM------------------------QKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALP 244          

HSP 2 Score: 69.707 bits (169), Expect = 4.303e-11
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F   G V + D+K + +GKS+G G V+FE+PE A  A  + N   +S REI +R+D+
Sbjct:  615 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKME-NGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDR 688          

HSP 3 Score: 61.6178 bits (148), Expect = 1.277e-8
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 3
Query:  459 FIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            FI N+ + + WQ LKD+ K   G V +V+L  DA+GKSRG   VEF+  E    A  + N   +S R +K++ D  G+ A
Sbjct:   73 FITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA 152          

HSP 4 Score: 56.9954 bits (136), Expect = 3.793e-7
Identity = 25/75 (33.33%), Postives = 43/75 (57.33%), Query Frame = 3
Query: 1386 SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            ++ + N+ +   WK LK  FS A  ++  +I  D   +S+GIG V FE   +AV+  +M +G L   RP+H++ +
Sbjct:  165 TVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMD 239          

HSP 5 Score: 56.225 bits (134), Expect = 7.431e-7
Identity = 25/74 (33.78%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF +TWK LK  F++   +++ +I  ++  +SKG G+V+FE+   A R   MM+G    GR I +  +
Sbjct:  615 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKMEN-GKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRID 687          
BLAST of Myelin expression factor 2 isoform 2 vs. UniProt/SwissProt
Match: sp|Q8C854|MYEF2_MOUSE (Myelin expression factor 2 OS=Mus musculus OX=10090 GN=Myef2 PE=1 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.446e-29
Identity = 72/211 (34.12%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNF--SSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK  G  V+EFK  +  +KA++ M+KY ++GR + ++E+ + +  R +L     +F  S A      L NL              I+ L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE   EAV AI +FN + + +R + ++MD    P
Sbjct:   95 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGTSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQA-GRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVP 304          

HSP 2 Score: 73.559 bits (179), Expect = 2.106e-12
Identity = 35/81 (43.21%), Postives = 50/81 (61.73%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            G   N +F+ NL + LTWQKLK+ F   G V   ++K + +GKS+G GTV FE+ E A  A  + N   +S REI +R+D+
Sbjct:  510 GSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKME-NGKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLDR 589          

HSP 3 Score: 68.9366 bits (167), Expect = 6.027e-11
Identity = 36/90 (40.00%), Postives = 53/90 (58.89%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK GP  N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSRG G VEF+  E    A+   N   +S R + ++ D  G+ A
Sbjct:   84 EKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENA 173          

HSP 4 Score: 58.9214 bits (141), Expect = 9.806e-8
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF  TW+ LK  FSQ   +M  EI  ++  +SKG G VRFE+   A +   +M+G    GR I +  +
Sbjct:  516 IFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMEN-GKSKGCGTVRFESAESAEKACRIMNGIKISGREIDVRLD 588          

HSP 5 Score: 53.9138 bits (128), Expect = 3.373e-6
Identity = 33/119 (27.73%), Postives = 57/119 (47.90%), Query Frame = 3
Query: 1278 SSVMNKRNDDPYSGINQLHP----NPSRPN---NDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            +S       D  SG+  L P    NP+ P    ++  +     ++ + N+ F   WK LK  FS A  +   +I  D   +S+G+G V FE   +AV+  +M +G     RP+H++ ++
Sbjct:  182 TSFQGSHASDVGSGLVNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDD 300          
BLAST of Myelin expression factor 2 isoform 2 vs. UniProt/SwissProt
Match: sp|P52272|HNRPM_HUMAN (Heterogeneous nuclear ribonucleoprotein M OS=Homo sapiens OX=9606 GN=HNRNPM PE=1 SV=3)

HSP 1 Score: 122.865 bits (307), Expect = 5.470e-28
Identity = 74/211 (35.07%), Postives = 122/211 (57.82%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG---------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ITNIPF+   + LKD+ K+++G    ++ + D  GK  G AV+EFK  +  +KA + ++K+S++GR + V+E+ + +  R ++          G   G         S+ N       +I+ L+  G + +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV AI +FN +++ +R + ++MD+   P
Sbjct:   75 ITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQA-GRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALP 284          

HSP 2 Score: 69.707 bits (169), Expect = 4.343e-11
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F   G V + D+K + +GKS+G G V+FE+PE A  A  + N   +S REI +R+D+
Sbjct:  655 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKME-NGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDR 728          

HSP 3 Score: 62.003 bits (149), Expect = 1.154e-8
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 3
Query:  459 FIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            FI N+ + + WQ LKD+ K   G V +V+L  DA+GKSRG   VEF+  E    A  + N   +S R +K++ D  G+ A
Sbjct:   74 FITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA 153          

HSP 4 Score: 60.077 bits (144), Expect = 3.895e-8
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 3
Query: 1338 NPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NP+ PN    +  A +   ++ + N+ +   WK LK  FS A  ++  +I  D   +S+GIG V FE   +AV+  +M +G L   RP+H++ +
Sbjct:  186 NPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMD 279          

HSP 5 Score: 56.225 bits (134), Expect = 7.230e-7
Identity = 25/74 (33.78%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF +TWK LK  F++   +++ +I  ++  +SKG G+V+FE+   A R   MM+G    GR I +  +
Sbjct:  655 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKMEN-GKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRID 727          
BLAST of Myelin expression factor 2 isoform 2 vs. UniProt/SwissProt
Match: sp|Q9D0E1|HNRPM_MOUSE (Heterogeneous nuclear ribonucleoprotein M OS=Mus musculus OX=10090 GN=Hnrnpm PE=1 SV=3)

HSP 1 Score: 121.324 bits (303), Expect = 1.835e-27
Identity = 73/211 (34.60%), Postives = 120/211 (56.87%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQ----------PQGSLFN------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ITNIPF+   + LKD+ K+++G    ++ + D  GK  G AV+EFK  +  +KA + ++K+S++GR + V+E+ + +  R ++          G           P   L N      +I+ L+  G + +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG G V FE   EAV AI +FN +++ +R + ++MD+   P
Sbjct:   74 ITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMINIPPSILNNPNIPNEIIHALQA-GRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGIVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALP 283          

HSP 2 Score: 69.707 bits (169), Expect = 3.844e-11
Identity = 32/75 (42.67%), Postives = 48/75 (64.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F   G V + D+K + +GKS+G G V+FE+PE A  A  + N   +S REI +R+D+
Sbjct:  654 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKME-NGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDR 727          

HSP 3 Score: 62.3882 bits (150), Expect = 9.457e-9
Identity = 32/80 (40.00%), Postives = 46/80 (57.50%), Query Frame = 3
Query:  459 FIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            FI N+ + + WQ LKD+ K   G V +V+L  DA+GKSRG   VEF+  E    A  + N   +S R +K++ D  G+ A
Sbjct:   73 FITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHA 152          

HSP 4 Score: 61.6178 bits (148), Expect = 1.533e-8
Identity = 30/94 (31.91%), Postives = 52/94 (55.32%), Query Frame = 3
Query: 1338 NPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NP+ PN    +  A +   ++ + N+ +   WK LK  FS A  ++  +I  D   +S+GIG+V FE   +AV+  +M +G L   RP+H++ +
Sbjct:  185 NPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGIVTFEQSIEAVQAISMFNGQLLFDRPMHVKMD 278          

HSP 5 Score: 56.225 bits (134), Expect = 6.803e-7
Identity = 25/74 (33.78%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF +TWK LK  F++   +++ +I  ++  +SKG G+V+FE+   A R   MM+G    GR I +  +
Sbjct:  654 IFVRNLPFDFTWKMLKDKFNECGHVLYADIKMEN-GKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRID 726          
BLAST of Myelin expression factor 2 isoform 2 vs. TrEMBL
Match: A0A183PSS2 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS17410 PE=4 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 4.572e-47
Identity = 82/206 (39.81%), Postives = 142/206 (68.93%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERL-SLSTPQHNFSSAGQP--------QGSLFN--LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            I NIP++   ++LK++F++++G  G L    KNGK +G  +MEFKT++GAQKA++ MH++ +  R+++VR+ET +D  R  S+       S +G P         GS+F   ++ ++  DGPIT+S++++NLDY++TWQKLKD+FK+AG++    +KTD++G SRG G ++F    EAV A+++FN +++ +R +++++D+ G PA
Sbjct:   43 IANIPYDLNWQKLKELFREQIGFTGFLQLFKKNGKPNGMGLMEFKTLEGAQKAIEMMHRFEVGDRKLVVRQETARDAARFASMEVDGGLLSDSGNPNESVNMAAAGSVFTPQILGQVGMDGPITDSVYVSNLDYSVTWQKLKDVFKSAGKILSSVIKTDSEGNSRGVGILKFSNAYEAVQAVNMFNNQILKDRPMRVKIDRQGTPA 248          
BLAST of Myelin expression factor 2 isoform 2 vs. TrEMBL
Match: A0A3Q0KEV8 (Rna recognition motif (Rrm) domain containing protein, putative OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 9.468e-46
Identity = 80/206 (38.83%), Postives = 141/206 (68.45%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERL-SLSTPQHNFSSAGQP--------QGSLFN--LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            I NIP++   ++LK++F++++G  G L    KNGK +G  +MEFKT++GAQKA++ MH++ +  R+++VR+ET +D  RL S+       S +  P         G +F   ++ ++  DGP+T+S++++NLDY++TWQKLKD+FK+AG++    +KTD++G SRG G ++F    EAV A+++FN +++ +R +++++D+ G PA
Sbjct:   43 IANIPYDLNWQKLKELFREQIGFTGFLQLFKKNGKPNGMGLMEFKTLEGAQKAIETMHRFEVGDRKLVVRQETARDAARLASMEVDGGLLSDSTNPNESVNMAATGPVFTPQMLGQVGIDGPVTDSVYVSNLDYSVTWQKLKDVFKSAGKILSAVIKTDSEGNSRGVGILKFSNAYEAVQAVNMFNNQILKDRPMRVKIDRQGTPA 248          
BLAST of Myelin expression factor 2 isoform 2 vs. TrEMBL
Match: Q86EB1 (Clone ZZD61 mRNA sequence OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 2.089e-45
Identity = 78/205 (38.05%), Postives = 139/205 (67.80%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQ--------GSLFN--LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            I NIP++   ++LK++F++++G  G L     NG+ +G  +MEFKT++GAQKA++ MH++ I  R+++VREET +D  R++      +  S   P         G ++   +++++  +GP+T+S++++NLDY++TW+KLKD+FK AG+V    +KTDA+G SRG G ++F  P EAV A+ +FN +++ +R +++++D+ G PA
Sbjct:   43 IANIPYDLNWQKLKELFREQIGFTGFLQLFKVNGRPNGMGLMEFKTLEGAQKAIETMHRFEIGDRKLVVREETARDAARIASMEVDGSLPSDSNPSDSTNTVSSGPIYTPQVLSQMGIEGPVTDSVYVSNLDYSVTWRKLKDVFKLAGKVTSSIIKTDAEGNSRGVGILKFSHPYEAVQAVSMFNNQILKDRPMRVKIDRQGTPA 247          
BLAST of Myelin expression factor 2 isoform 2 vs. TrEMBL
Match: Q5DC86 (SJCHGC01283 protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 5.932e-45
Identity = 78/205 (38.05%), Postives = 139/205 (67.80%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQ--------GSLFN--LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            I NIP++   ++LK++F++++G  G L     NG+ +G  +MEFKT++GAQKA++ MH++ I  R+++VREET +D  R++      +  S   P         G ++   +++++  +GP+T+S++++NLDY++TW+KLKD+FK AG+V    +KTDA+G SRG G ++F  P EAV A+ +FN +++ +R +++++D+ G PA
Sbjct:   43 IANIPYDLNWQKLKELFREQIGFTGFLQLFKVNGRPNGMGLMEFKTLEGAQKAIETMHRFEIGDRKLVVREETARDAARIASMEVDGSLPSDSNPSDSTNTVSSGPIYTPQVLSQMGIEGPVTDSVYVSNLDYSVTWRKLKDVFKLAGKVTSSIIKTDAEGNSRGVGILKFSHPYEAVQAVSMFNNQILKDRPMRVKIDRQGTPA 247          
BLAST of Myelin expression factor 2 isoform 2 vs. TrEMBL
Match: A0A183KQH1 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS17306 PE=4 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 1.170e-44
Identity = 81/206 (39.32%), Postives = 141/206 (68.45%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERL-SLSTPQHNFSSAGQP--------QGSLFN--LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            I NIP++   ++LK++F++++G  G L    KNGK +G  +MEFKT++GAQKA++ MH++ +  R+++VR+ET +D  R  S+       S +G P         G +F   ++ ++  DGPIT+S++++NLDY++TWQKLKD+FK+AG++    +KTD++G SRG G ++F    EAV A+++FN +++ +R +++++D+ G PA
Sbjct:   43 IANIPYDLNWQKLKELFREQIGFTGFLQLFKKNGKPNGMGLMEFKTLEGAQKAIEMMHRFEVGDRKLVVRQETARDAARFASMEVDGGLLSDSGNPNESVNMAAAGPVFTPQILGQVGMDGPITDSVYVSNLDYSVTWQKLKDVFKSAGKILSSVIKTDSEGNSRGVGILKFSNAYEAVQAVNMFNNQILKDRPMRVKIDRQGTPA 248          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Cavefish
Match: myef2 (myelin expression factor 2 [Source:NCBI gene;Acc:103029356])

HSP 1 Score: 131.724 bits (330), Expect = 3.857e-32
Identity = 78/229 (34.06%), Postives = 134/229 (58.52%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSA-GQPQG--------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQ 746
            I+NIP++   + +KD+ +D++G    ++ + D  GK   + V+EFK  +  +KA++ M+K+ +NGR + ++E+ + +  R  L      +S   GQ  G        S+ N       +IN L+  G + +++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV FE P EAV AI +FN +M+ +R++ ++MD+   P      +  F +++  PQ
Sbjct:   91 ISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSGVVEFKDEEFVKKALEVMNKHDLNGRPLNIKEDPDGEHARRVLQRSGGMYSGGRGQDSGPMGMNVPPSIANNPNIPPEIINALQ-GGRLGSTVFVANLDFKVGWKKLKEVFGMAGTVKRADVKEDKDGKSRGMGTVTFEQPLEAVQAISMFNGQMLFDRQMHVKMDEKSVP------MDDFRSVEKSPQ 312          

HSP 2 Score: 74.7146 bits (182), Expect = 1.189e-13
Identity = 33/75 (44.00%), Postives = 51/75 (68.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL Y LTWQKLK+ F + G+V   ++K + +GKS+G GTV F++PE A  A  L N   ++ RE+ +R+D+
Sbjct:  497 IFVRNLSYDLTWQKLKEKFGHCGQVMFAEIKME-NGKSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRLDR 570          

HSP 3 Score: 61.2326 bits (147), Expect = 1.791e-9
Identity = 43/133 (32.33%), Postives = 69/133 (51.88%), Query Frame = 3
Query:  450 NSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKAL 845
            N +FI+N+ Y + WQ +KD+ ++  G V +V+L  DA+GKSR  G VEF+  E    A+ + N   ++ R + ++ D  G+ A  +  + R G + SG +     P    V   I     NPN   P+ + AL
Sbjct:   87 NRVFISNIPYDMKWQAIKDLMRDKVGEVTYVELFKDAEGKSRVSGVVEFKDEEFVKKALEVMNKHDLNGRPLNIKEDPDGEHA--RRVLQRSGGMYSGGRGQDSGPMGMNVPPSI---ANNPN-IPPEIINAL 213          

HSP 4 Score: 50.8322 bits (120), Expect = 3.092e-6
Identity = 22/74 (29.73%), Postives = 41/74 (55.41%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+ +  TW+ LK  F     +M  EI  ++  +SKG G VRF++   A +   +M+GT  +GR + +  +
Sbjct:  497 IFVRNLSYDLTWQKLKEKFGHCGQVMFAEIKMEN-GKSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRLD 569          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Cavefish
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:ZFIN;Acc:ZDB-GENE-030131-6898])

HSP 1 Score: 85.1149 bits (209), Expect = 6.680e-17
Identity = 38/85 (44.71%), Postives = 57/85 (67.06%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV A+ + N +++ NR + +++D+   P
Sbjct:  214 GKIGSTVFVANLDYKVGWKKLKEVFGMAGVVVRADILEDKDGKSRGMGTVTFEMAIEAVQAVSMLNGQLLFNRTMHVKLDEKSLP 298          

HSP 2 Score: 65.855 bits (159), Expect = 6.465e-11
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F + G V++ D+K + +GKS+G G V F+ PE A  A    N   ++ REI +R+D+
Sbjct:  601 IFVRNLPFDFTWKMLKDAFNSCGMVQYADIKME-NGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDR 674          

HSP 3 Score: 57.3806 bits (137), Expect = 3.429e-8
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F      CG + Y D   +NGK  G  V+ F   + A++A + M+ Y +NGREI VR + N
Sbjct:  603 VRNLPFDFTWKMLKDAFNS----CGMVQYADIKMENGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDRN 675          

HSP 4 Score: 53.1434 bits (126), Expect = 6.828e-7
Identity = 28/97 (28.87%), Postives = 49/97 (50.52%), Query Frame = 3
Query: 1329 LHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            L  NP+ P        A K   ++ + N+ +   WK LK  F  A  ++  +I  D   +S+G+G V FE   +AV+  +M++G L   R +H++ +
Sbjct:  197 LMNNPNIPTEIIHGLQAGKIGSTVFVANLDYKVGWKKLKEVFGMAGVVVRADILEDKDGKSRGMGTVTFEMAIEAVQAVSMLNGQLLFNRTMHVKLD 293          

HSP 5 Score: 52.373 bits (124), Expect = 1.125e-6
Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 3
Query: 1374 NASKS--LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NA K   + ++N+PF +TWK LK +F+    + + +I  ++  +SKG G+VRF+    A R    M+G   +GR I +  +
Sbjct:  594 NARKGCQIFVRNLPFDFTWKMLKDAFNSCGMVQYADIKMEN-GKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRID 673          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Cavefish
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:ZFIN;Acc:ZDB-GENE-030131-6898])

HSP 1 Score: 85.1149 bits (209), Expect = 6.758e-17
Identity = 38/85 (44.71%), Postives = 57/85 (67.06%), Query Frame = 3
Query:  438 GPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE   EAV A+ + N +++ NR + +++D+   P
Sbjct:  214 GKIGSTVFVANLDYKVGWKKLKEVFGMAGVVVRADILEDKDGKSRGMGTVTFEMAIEAVQAVSMLNGQLLFNRTMHVKLDEKSLP 298          

HSP 2 Score: 65.855 bits (159), Expect = 6.964e-11
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F + G V++ D+K + +GKS+G G V F+ PE A  A    N   ++ REI +R+D+
Sbjct:  597 IFVRNLPFDFTWKMLKDAFNSCGMVQYADIKME-NGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDR 670          

HSP 3 Score: 57.3806 bits (137), Expect = 3.632e-8
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F      CG + Y D   +NGK  G  V+ F   + A++A + M+ Y +NGREI VR + N
Sbjct:  599 VRNLPFDFTWKMLKDAFNS----CGMVQYADIKMENGKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRIDRN 671          

HSP 4 Score: 53.1434 bits (126), Expect = 6.811e-7
Identity = 28/97 (28.87%), Postives = 49/97 (50.52%), Query Frame = 3
Query: 1329 LHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            L  NP+ P        A K   ++ + N+ +   WK LK  F  A  ++  +I  D   +S+G+G V FE   +AV+  +M++G L   R +H++ +
Sbjct:  197 LMNNPNIPTEIIHGLQAGKIGSTVFVANLDYKVGWKKLKEVFGMAGVVVRADILEDKDGKSRGMGTVTFEMAIEAVQAVSMLNGQLLFNRTMHVKLD 293          

HSP 5 Score: 52.373 bits (124), Expect = 1.256e-6
Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 3
Query: 1374 NASKS--LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            NA K   + ++N+PF +TWK LK +F+    + + +I  ++  +SKG G+VRF+    A R    M+G   +GR I +  +
Sbjct:  590 NARKGCQIFVRNLPFDFTWKMLKDAFNSCGMVQYADIKMEN-GKSKGCGVVRFDNPETAERACRTMNGYRLNGREIDVRID 669          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Cavefish
Match: ENSAMXT00000035946.1 (polyadenylate-binding protein 1-like [Source:NCBI gene;Acc:103030422])

HSP 1 Score: 63.5438 bits (153), Expect = 3.332e-10
Identity = 46/178 (25.84%), Postives = 84/178 (47.19%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            ++LKDVF             D++GK  G   + F+  + AQ+AV EM+   +NG+++ V     K   +  L   +  F    Q + + +  +N           L++ NLD  L  ++L+  F   G +    +  +  G+S+GFG V F +PEEA  A+   N ++V+ + + + +
Sbjct:  205 EKLKDVFGKYGPALSIRVMTDESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQKKGERQTEL---KRKFEQMKQDRMTRYQGVN-----------LYVKNLDDGLDDERLRKEFSPFGTITSAKVMMEG-GRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVAL 367          

HSP 2 Score: 59.3066 bits (142), Expect = 7.317e-9
Identity = 49/185 (26.49%), Postives = 84/185 (45.41%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIV-REETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKL-RMDKSGK 689
            K L D F     I       D+NG   G   + F+T + A++A+++M+   +N R++ V R ++ K+RE                          E+        +++I N    +  +KLKD+F   G    + + TD  GKS+GFG V FE  E+A  A+   N K ++ +++ + R  K G+
Sbjct:  113 KALYDTFSAFGNILSCKVVCDENGS-KGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKERE-------------------------AEMGARAKEFTNVYIKNFGEDMDDEKLKDVFGKYGPALSIRVMTDESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQKKGE 271          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Cavefish
Match: ENSAMXT00000018970.2 (polyadenylate-binding protein 1-like [Source:NCBI gene;Acc:103030422])

HSP 1 Score: 62.3882 bits (150), Expect = 8.150e-10
Identity = 52/211 (24.64%), Postives = 97/211 (45.97%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLD---VSRFGNIDSGPQNNTLSP 764
            ++LKDVF             D++GK  G   + F+  + AQ+AV EM+   +NG+++ V     K   +  L   +  F    Q + + +  +N           L++ NLD  L  ++L+  F   G +    +  +  G+S+GFG V F +PEEA  A+   N ++V+ + + + + +  +     L    + R  ++ + P N  L+P
Sbjct:  205 EKLKDVFGKYGPALSIRVMTDESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQKKGERQTEL---KRKFEQMKQDRMTRYQGVN-----------LYVKNLDDGLDDERLRKEFSPFGTITSAKVMMEG-GRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVP-NPVLNP 399          

HSP 2 Score: 58.5362 bits (140), Expect = 1.156e-8
Identity = 51/195 (26.15%), Postives = 88/195 (45.13%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIV-REETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKL-RMDKSGK 689
            I N+  +   K L D F     I       D+NG   G   + F+T + A++A+++M+   +N R++ V R ++ K+RE                          E+        +++I N    +  +KLKD+F   G    + + TD  GKS+GFG V FE  E+A  A+   N K ++ +++ + R  K G+
Sbjct:  103 IKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGS-KGYGFVHFETHEAAERAIEKMNGMLLNDRKVFVGRFKSRKERE-------------------------AEMGARAKEFTNVYIKNFGEDMDDEKLKDVFGKYGPALSIRVMTDESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYVGRAQKKGE 271          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Sea Lamprey
Match: myef2 (myelin expression factor 2 [Source:ZFIN;Acc:ZDB-GENE-051120-114])

HSP 1 Score: 83.1889 bits (204), Expect = 4.455e-17
Identity = 35/93 (37.63%), Postives = 62/93 (66.67%), Query Frame = 3
Query:  414 LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            L+N L+  G +  ++F+ NLDY + W+K+K++F  AG V+  ++K D +G+SRG G + +E P EA+ AI +FN +++  R++ ++MD    P
Sbjct:   69 LLNALQS-GRLGTTVFVTNLDYKVGWKKIKEVFGMAGVVKRAEIKEDKEGRSRGMGVIIYEQPVEALQAISMFNGQLLFERQMHVKMDDRSLP 160          

HSP 2 Score: 75.485 bits (184), Expect = 1.391e-14
Identity = 33/75 (44.00%), Postives = 51/75 (68.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL + L+WQKLK+ F   G+V   D+K + +G+S+G GTV F++PE A  A H+ N   ++ REI +R+DK
Sbjct:  488 IFVRNLAFDLSWQKLKETFSQCGQVMFADIKKE-NGRSKGCGTVRFDSPEAAEKACHMLNGIRINGREIDVRIDK 561          

HSP 3 Score: 54.299 bits (129), Expect = 5.337e-8
Identity = 25/80 (31.25%), Postives = 46/80 (57.50%), Query Frame = 3
Query: 1371 DNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            D     + ++N+ F  +W+ LK +FSQ   +M  +I +++  RSKG G VRF++   A +  +M++G   +GR I +  +
Sbjct:  482 DRRGCQIFVRNLAFDLSWQKLKETFSQCGQVMFADIKKEN-GRSKGCGTVRFDSPEAAEKACHMLNGIRINGREIDVRID 560          

HSP 4 Score: 48.521 bits (114), Expect = 3.502e-6
Identity = 25/95 (26.32%), Postives = 48/95 (50.53%), Query Frame = 3
Query: 1338 NPSRPN---NDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNN 1613
            NP+ P    N   S     ++ + N+ +   WK +K  F  A  +   EI  D   RS+G+G++ +E   +A++  +M +G L   R +H++ ++
Sbjct:   62 NPNIPPELLNALQSGRLGTTVFVTNLDYKVGWKKIKEVFGMAGVVKRAEIKEDKEGRSRGMGVIIYEQPVEALQAISMFNGQLLFERQMHVKMDD 156          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Sea Lamprey
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663])

HSP 1 Score: 63.929 bits (154), Expect = 5.619e-11
Identity = 46/175 (26.29%), Postives = 82/175 (46.86%), Query Frame = 3
Query:  138 MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            ++E    F   L +C      D+ GK  G   + F+  + AQ+AV EM+   +NGR + V     K   +  L   +  F    Q + S +  +N           L++ NLD  +  ++L+  F   G +    + ++ +G+S+GFG V F +PEEA  A+   N ++V ++ +
Sbjct:  210 LREFFGKFGKALSVC---VMSDECGKSRGFGFVSFEQHEDAQRAVNEMNGKELNGRTVFVGRAQKKGERQAEL---KRKFEMLKQERISRYQGVN-----------LYVKNLDDNIDDEQLRKEFAPYGTITSTKVMSE-EGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPL 366          

HSP 2 Score: 51.6026 bits (122), Expect = 3.968e-7
Identity = 49/198 (24.75%), Postives = 85/198 (42.93%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGR----EIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKL-RMDKSGK 689
            I N+  +   K + D F     I       D+NG   G   + F+T + A +A+++M+   +N R      + R ++ K+RE+                         E+        +++I N    +   KL++ F   G+   V + +D  GKSRGFG V FE  E+A  A++  N K ++ R + + R  K G+
Sbjct:  103 IKNLDKSIDNKAMYDTFSAFGNILSCKVVCDENGS-RGYGFVHFETQEAADRAIEKMNGMLLNDRLARRRFVGRFKSRKEREQ-------------------------EMGARAKEFTNVYIKNFGDDMDEDKLREFFGKFGKALSVCVMSDECGKSRGFGFVSFEQHEDAQRAVNEMNGKELNGRTVFVGRAQKKGE 274          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Sea Lamprey
Match: pabpc4 (poly(A) binding protein, cytoplasmic 4 (inducible form) [Source:ZFIN;Acc:ZDB-GENE-030131-9663])

HSP 1 Score: 63.5438 bits (153), Expect = 5.991e-11
Identity = 47/179 (26.26%), Postives = 85/179 (47.49%), Query Frame = 3
Query:  147 LKDVFKDRLGICGKLDYI-DKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            L++ F+  +G    +  + D+ GK  G   + F+  + AQKAV EM+   +NGR I V     K   +  L   +  F    Q + S +  +N           L++ NLD  +  ++L   F   G +    +  + +G+SRGFG V F +PEEA  A+   N +++ ++ + + + +
Sbjct:   67 LREYFEQYVGKTLSVKVMMDEGGKSKGFGFVSFERHEDAQKAVDEMNTKELNGRAIYVGRAQKKAERQTEL---KRKFEMLKQERMSKYQGVN-----------LYVKNLDDNINDERLWKEFSPFGTITSAKVMME-EGRSRGFGFVCFSSPEEATKAVTEMNGRIIGSKPLYVALAQ 230          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Sea Lamprey
Match: ncl (nucleolin [Source:ZFIN;Acc:ZDB-GENE-030131-6986])

HSP 1 Score: 56.9954 bits (136), Expect = 9.823e-9
Identity = 49/189 (25.93%), Postives = 88/189 (46.56%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHV-DLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            + NI +++T++ L + F+  + +   L     NGK  G A +EF + + A+ A+KE     ++GR +++    +K              S+  +PQ             GP +N+L + NL +      L+ +F+NA  VR + D +T   GK RG   V+F + ++A  A+   +   V  R I+L  
Sbjct:  376 VKNIAWSSTVESLTEAFEGAVEVRIPLG---PNGKSRGIAYVEFPSEEDAEAAMKEYQGADVDGRAVLLDYVGDK--------------SNKAKPQ-------------GPESNTLMVRNLSFQADEDSLRSVFENAEAVRVLMDRET---GKPRGMAFVDFASIDDAKAAMKAHSGAEVEGRAIRLEF 531          

HSP 2 Score: 46.9802 bits (110), Expect = 9.937e-6
Identity = 48/187 (25.67%), Postives = 80/187 (42.78%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGP--ITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDAD-GKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            + N+ F      L+ VF++   +   +D   + GK  G A ++F ++D A+ A+K      + GR I           RL  STP+      G P              GP   +++LF+  L    +   L D F+ A   R V   TD D G+S+G+  V+F + +EA  A++      +   +I
Sbjct:  463 VRNLSFQADEDSLRSVFENAEAVRVLMDR--ETGKPRGMAFVDFASIDDAKAAMKAHSGAEVEGRAI-----------RLEFSTPR------GTP--------------GPSEASSTLFVRGLGEDTSEDTLMDSFEGATAARIV---TDRDSGQSKGYAYVDFSSADEAKSALNAMKGVEIDGSQI 613          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Yeast
Match: GBP2 (Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication [Source:SGD;Acc:S000000517])

HSP 1 Score: 82.4185 bits (202), Expect = 2.687e-17
Identity = 54/187 (28.88%), Postives = 89/187 (47.59%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLR 671
            + N+ F+ T ++LK++F   +G   + D I   G   G   +EF   +  Q A+ +        R+++VR++            P    +     + +   + N  E        +FI NL Y++ WQ LKDMFK  G V   D++ D +G SRGFG+V + T +E + AI  FN   V  R +++R
Sbjct:  126 VRNLTFDCTPEDLKELFG-TVGEVVEADIITSKGHHRGMGTVEFTKNESVQDAISKFDGALFMDRKLMVRQDN-----------PPPEAAKEFSKKATREEIDNGFE--------VFIINLPYSMNWQSLKDMFKECGHVLRADVELDFNGFSRGFGSVIYPTEDEMIRAIDTFNGMEVEGRVLEVR 292          

HSP 2 Score: 47.3654 bits (111), Expect = 4.423e-6
Identity = 43/192 (22.40%), Postives = 78/192 (40.62%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKL----DYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVRE-------------ETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFN 635
            I N+P++   + LKD+FK+    CG +      +D NG   G   + + T D   +A+   +   + GR + VRE             +  +D E    + P     +A     +       +   G     ++ +NL ++     L D+F   G++ + +LK   +G+  G   VE+E   +A   I   N
Sbjct:  223 IINLPYSMNWQSLKDMFKE----CGHVLRADVELDFNGFSRGFGSVIYPTEDEMIRAIDTFNGMEVEGRVLEVREGRFNKRKNNDRYNQRREDLEDTRGTEPGLAQDAAVHIDETAAKFTEGVNPGGDRNCFIYCSNLPFSTARSDLFDLFGPIGKINNAELKPQENGQPTGVAVVEYENLVDADFCIQKLN 410          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Yeast
Match: HRB1 (Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication [Source:SGD;Acc:S000004949])

HSP 1 Score: 70.8626 bits (172), Expect = 1.567e-13
Identity = 50/195 (25.64%), Postives = 90/195 (46.15%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREE----TNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKS 683
            + N+ +++T ++L + F  ++G   + D I   G   G   +EF   D   +A+++        R+I VR++    +N  +ER +L             +G L        +    T+ + + NL  ++ WQ LKD+FK  G V H D++ D DG S G GTV F   ++   AI  +N   +    + ++  +S
Sbjct:  165 VGNLTYDSTPEDLTEFFS-QIGKVVRADIITSRGHHRGMGTVEFTNSDDVDRAIRQYDGAFFMDRKIFVRQDNPPPSNNIKERKALD------------RGEL--------RHNRKTHEVIVKNLPASVNWQALKDIFKECGNVAHADVELDGDGVSTGSGTVSFYDIKDLHRAIEKYNGYSIEGNVLDVKSKES 338          

HSP 2 Score: 58.151 bits (139), Expect = 1.908e-9
Identity = 40/181 (22.10%), Postives = 87/181 (48.07%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDY----IDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVR--EETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFN 635
            + N+P +   + LKD+FK+    CG + +    +D +G  +G+  + F  +    +A+++ + YSI G  + V+  E  +   +   +  P  +     + +    N++   E++  I    + +NL ++     L D+F+  G+V + +L+ D+ G   G   VE++  ++A + I   N
Sbjct:  265 VKNLPASVNWQALKDIFKE----CGNVAHADVELDGDGVSTGSGTVSFYDIKDLHRAIEKYNGYSIEGNVLDVKSKESVHNHSDGDDVDIPMDDSPVNEEARKFTENVVGGGERNRLI----YCSNLPFSTAKSDLYDLFETIGKVNNAELRYDSKGAPTGIAVVEYDNVDDADVCIERLN 437          

HSP 3 Score: 51.6026 bits (122), Expect = 1.943e-7
Identity = 27/77 (35.06%), Postives = 42/77 (54.55%), Query Frame = 3
Query:  447 TNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMD 677
            +NS+F+ NL Y  T + L + F   G+V   D+ T + G  RG GTVEF   ++   AI  ++     +R+I +R D
Sbjct:  160 SNSIFVGNLTYDSTPEDLTEFFSQIGKVVRADIIT-SRGHHRGMGTVEFTNSDDVDRAIRQYDGAFFMDRKIFVRQD 235          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Yeast
Match: PAB1 (Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex [Source:SGD;Acc:S000000967])

HSP 1 Score: 62.3882 bits (150), Expect = 1.068e-10
Identity = 50/189 (26.46%), Postives = 89/189 (47.09%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITN-SLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            + NI   TT ++ +++F     I       D +GK  G   + ++  + A KAV+ ++   +NG ++ V     K+ ER+ +   Q+                  LEK       +LF+ NLD ++  +KL++ F   G +    +    +GKS+GFG V F TPEEA  AI   N ++V+ + + + +
Sbjct:  223 VKNINSETTDEQFQELFAKFGPIVSASLEKDADGKLKGFGFVNYEKHEDAVKAVEALNDSELNGEKLYVGRAQKKN-ERMHVLKKQYE--------------AYRLEKMAKYQGVNLFVKNLDDSVDDEKLEEEFAPYGTITSAKVMRTENGKSKGFGFVCFSTPEEATKAITEKNQQIVAGKPLYVAI 396          

HSP 2 Score: 59.6918 bits (143), Expect = 7.014e-10
Identity = 46/175 (26.29%), Postives = 79/175 (45.14%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFN 635
            I N+  +   K L D F     I       D+NGK  G   + F+    A++A+  ++   +NG+EI V    ++ +ER S                       +LE+      +L++ N++   T ++ +++F   G +    L+ DADGK +GFG V +E  E+AV A+   N
Sbjct:  130 IKNLHPDIDNKALYDTFSVFGDILSSKIATDENGKSKGFGFVHFEEEGAAKEAIDALNGMLLNGQEIYVAPHLSR-KERDS-----------------------QLEETKAHYTNLYVKNINSETTDEQFQELFAKFGPIVSASLEKDADGKLKGFGFVNYEKHEDAVKAVEALN 280          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Yeast
Match: NOP13 (Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress [Source:SGD;Acc:S000005119])

HSP 1 Score: 47.7506 bits (112), Expect = 3.017e-6
Identity = 52/243 (21.40%), Postives = 105/243 (43.21%), Query Frame = 3
Query:  213 KFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDAD-GKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM--DKSGKPALGKL-----------DVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQY 899
            K  G   M FK V+   KAV E+ +  +NGR +++++  N                 +G+P       +++     P +  LF+ NL + +T   L+  F++ G +  + + T  D GK +GF  ++F+  E +  A+   + + ++ R +++    D+S +    K+           D+S     D   Q+N   P+ +R   +    V + NR+      A  +  S A +  +G+  ++
Sbjct:  181 KNKGFCYMFFKNVE-QMKAVLELSESHLNGRNMLIKDSEN----------------YSGRPDKDDLVAMSK----NPPSRILFVGNLSFDVTDDLLRKHFQHCGDIVKIRMATFEDSGKCKGFAFIDFKNEEGSTNALKDKSCRKIAGRPLRMEYGEDRSKRQVRKKVENVSRNNSSSFDISNNKGYDRAGQDNGSKPEYKRSNANRRPPVDSNNRTKSSVALATAQRGSAAIVPSQGKKVKF 402          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Yeast
Match: SGN1 (Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation [Source:SGD;Acc:S000001440])

HSP 1 Score: 46.9802 bits (110), Expect = 3.449e-6
Identity = 33/129 (25.58%), Postives = 64/129 (49.61%), Query Frame = 3
Query:  336 DRERLSLSTPQHNFSSAGQPQGSL-----FNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDAD-GKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGK 704
            D++ L L       S  G PQ S          ++LE D   + S+F+ N+   +T ++++D FK+ G+++ + L  D + G  +G+G +EFE+P     A+ L N   +  ++I +   ++  P   +
Sbjct:   24 DKQELELDELVGKLSIEGTPQVSQKLSKEEKHAHQLEAD---SRSIFVGNITPDVTPEQIEDHFKDCGQIKRITLLYDRNTGTPKGYGYIEFESPAYREKALQL-NGGELKGKKIAVSRKRTNIPGFNR 148          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Nematostella
Match: EDO45532 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSW7])

HSP 1 Score: 127.102 bits (318), Expect = 4.108e-31
Identity = 69/208 (33.17%), Postives = 118/208 (56.73%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSS--AGQPQGSLFNLIN------ELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKL 707
            ++NIPF +  + LKD     +G     + + D+ G+  G  V+EF + + A++ +   +    NGR ++VR    +D  R+  S  + N +S   G   G L +L+N        + + P  +++F+ NLDY + WQKLKD FK AG V   ++  D + KS+G GTV+FETP EA+ A++L + KM+ +R +++RMD++  P   ++
Sbjct:   97 VSNIPFESRWQNLKDHMNKVVGDVAFAEIFEDEKGRSKGCGVVEFTSSESAERCISLCNGQDFNGRNLLVRHTRPEDENRMHRSRLRGNSASQSVGGTGGGLPSLLNLSTNPMMKKSNDPAASTVFVTNLDYKVNWQKLKDTFKCAGHVIRAEIMEDDEKKSKGMGTVQFETPMEAMNAVNLLHGKMLMDRALRVRMDRAAPPVQNQM 304          

HSP 2 Score: 80.1073 bits (196), Expect = 8.293e-16
Identity = 37/77 (48.05%), Postives = 49/77 (63.64%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSG 686
            +F+ NL +  TWQ LKD F+ AG+V   D+ T+  G+S+G G V FET EEA MAI  FN      REI +R+D+ G
Sbjct:  472 VFVRNLPWKYTWQDLKDKFRPAGKVMRADILTEPSGRSKGCGIVVFETQEEAQMAISAFNGASFDGREIDVRLDRFG 548          

HSP 3 Score: 69.707 bits (169), Expect = 1.466e-12
Identity = 30/80 (37.50%), Postives = 46/80 (57.50%), Query Frame = 3
Query: 1371 DNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            D + K + ++N+P+ YTW+DLK  F  A  +M  +I  +   RSKG G+V FET  +A    +  +G  FDGR I +  +
Sbjct:  466 DASGKQVFVRNLPWKYTWQDLKDKFRPAGKVMRADILTEPSGRSKGCGIVVFETQEEAQMAISAFNGASFDGREIDVRLD 545          

HSP 4 Score: 59.6918 bits (143), Expect = 1.943e-9
Identity = 27/74 (36.49%), Postives = 43/74 (58.11%), Query Frame = 3
Query: 1383 KSLLIKNIPFSYTWKDLKHSFSQAPG-IMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHI 1601
            K + + NIPF   W++LK   ++  G +   EIF D+  RSKG G+V F +   A RC ++ +G  F+GR + +
Sbjct:   93 KKVFVSNIPFESRWQNLKDHMNKVVGDVAFAEIFEDEKGRSKGCGVVEFTSSESAERCISLCNGQDFNGRNLLV 166          

HSP 5 Score: 57.3806 bits (137), Expect = 1.055e-8
Identity = 24/80 (30.00%), Postives = 45/80 (56.25%), Query Frame = 3
Query: 1371 DNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            D A+ ++ + N+ +   W+ LK +F  A  ++  EI  DD  +SKG+G V+FET  +A+   N++ G +   R + +  +
Sbjct:  215 DPAASTVFVTNLDYKVNWQKLKDTFKCAGHVIRAEIMEDDEKKSKGMGTVQFETPMEAMNAVNLLHGKMLMDRALRVRMD 294          

HSP 6 Score: 49.6766 bits (117), Expect = 2.426e-6
Identity = 26/73 (35.62%), Postives = 40/73 (54.79%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKD-MFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLR 671
            +F++N+ +   WQ LKD M K  G V   ++  D  G+S+G G VEF + E A   I L N +  + R + +R
Sbjct:   95 VFVSNIPFESRWQNLKDHMNKVVGDVAFAEIFEDEKGRSKGCGVVEFTSSESAERCISLCNGQDFNGRNLLVR 167          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Nematostella
Match: EDO33206 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSV8])

HSP 1 Score: 60.4622 bits (145), Expect = 1.097e-9
Identity = 44/184 (23.91%), Postives = 80/184 (43.48%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            I N+  +   K L D F     I       D+ G   G   + F+T D A +A+  +    +N +++ V    +K      + T    F++                        +++ N    +  +++K++   AG++  + + TD +GKS+GFG V FETPEEA  A+++ N K +  R +
Sbjct:  105 IKNLDKSIDNKSLYDTFSAFGNILSCKIAQDELGNPKGYGFVHFETEDAALEAIARVDGMLLNDKKVFVGRWMSKKERIEKMGTQPKKFTN------------------------VYVKNFGDDMDDEQMKEICAEAGKIVSLKVMTDPEGKSKGFGFVSFETPEEAEEAVNVLNGKEIGGRRL 264          

HSP 2 Score: 51.6026 bits (122), Expect = 6.232e-7
Identity = 42/178 (23.60%), Postives = 80/178 (44.94%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            +++K++  +   I       D  GK  G   + F+T + A++AV  ++   I GR +       K  ER +    +       Q + + F  +N           L+I NLD  +  ++L++ F   G +    +  D  G S+GFG V F +PEEA  A+   N +++ ++ + + +
Sbjct:  208 EQMKEICAEAGKIVSLKVMTDPEGKSKGFGFVSFETPEEAEEAVNVLNGKEIGGRRLWA-GRAKKRAERAA--EVKAEIEKKRQERINRFQGVN-----------LYIKNLDDPIDDERLREEFSPYGTISSAKVMKDDKGNSKGFGFVCFSSPEEATKAVTEMNGRILISKPLYVAL 371          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Nematostella
Match: EDO37342 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFY3])

HSP 1 Score: 51.6026 bits (122), Expect = 6.998e-7
Identity = 52/191 (27.23%), Postives = 86/191 (45.03%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDY-IDKN--GKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            I+N+ F+ T   L+D F  +LG    LD  I KN  G+  G A +EF      Q A+  M +  + GR + +    +K                A  P  + F     L+K     ++LF++NL +     +++++F   G V+ V L T+  GK +G+G VE+E    A  A+   +   V  R I + +
Sbjct:  605 ISNLLFSITEDHLRDKF-SKLG--EVLDVRIVKNMAGRSKGYAYVEFNNESTVQAALA-MDREKMEGRPMFISPCVDK----------------AKNP--TTFKFPTSLDK-----HTLFVSNLPFDAKESEIEELFSKHGVVKQVRLVTNRAGKPKGYGYVEYEQESSASTAVLTLDKTEVKGRTISVAL 768          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Nematostella
Match: EDO47487 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLV0])

HSP 1 Score: 51.2174 bits (121), Expect = 9.279e-7
Identity = 49/193 (25.39%), Postives = 92/193 (47.67%), Query Frame = 3
Query:  120 IPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNS--LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDA-DGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGK 689
            +PF    K + + F     +  +   ++K G+ SG A ++F +    +KA+K    Y + GR I + ++TN+D +       + ++    Q +G       + E++ PI  S  LF+ NL Y+ + + ++ +F+  G +  V+L  D    K+ G G V F  PE AV A +  +  +   R + +   K+ K
Sbjct:  260 LPFKAKDKHILEFFSPLKPVAIRF-VMNKKGQPSGCAFVDFSSKSDLEKALKRNKDY-LQGRYIELFKDTNRDFDNNKQGDGEKSWMRKLQEKG-------DDEEEEPIGESGRLFLRNLAYSCSEEDIQHLFEKFGPLSEVNLPLDKHTNKTIGIGFVTFLMPEHAVKAFNELDGTVFQGRLLHILPAKAKK 443          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Nematostella
Match: EDO35243 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM09])

HSP 1 Score: 46.2098 bits (108), Expect = 1.133e-6
Identity = 25/74 (33.78%), Postives = 42/74 (56.76%), Query Frame = 3
Query:  453 SLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDAD-GKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLR 671
            S+F+ NLD  +T + L ++F  AG + +V + TD + G+ R FG VEF +P     A  L +   + +R I ++
Sbjct:    4 SVFVGNLDSRVTEEILWELFLQAGPLENVRIPTDKNTGQQRSFGFVEFSSPVSVHYASELLDGIRLYDRAINVK 77          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Medaka
Match: myef2 (myelin expression factor 2 [Source:NCBI gene;Acc:101173686])

HSP 1 Score: 124.79 bits (312), Expect = 7.448e-30
Identity = 73/210 (34.76%), Postives = 125/210 (59.52%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLD-YIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQG--------SLFN-------LINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            I+NIP++   + +KD+ ++++G    ++ + D  GK   + V+EFK  +  +KAV  M+K+ +NGR + ++E+ + +  R +L          GQ  G        S+ N       +I+ L+  G + N++F+ANLD+ + W+KLK++F  AG V+  D+K D DGKSRG GTV F+ P EAV AI +FN +M+ +R++ ++MD+   P
Sbjct:   85 ISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRVSGVVEFKDDEFVKKAVLTMNKHDLNGRPLNIKEDPDGEHARRALQRIGGGQGGRGQDMGPGGMNLPPSIANNPNIPPEVIHGLQA-GRLGNTVFVANLDFKVGWKKLKEVFGMAGVVKRADVKEDKDGKSRGMGTVTFDQPLEAVQAISMFNGQMLFDRQMHVKMDEKSLP 293          

HSP 2 Score: 73.1738 bits (178), Expect = 2.952e-13
Identity = 32/75 (42.67%), Postives = 51/75 (68.00%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL Y LTWQKLK+ F + G+V   ++K + +G+S+G GTV F++PE A  A  L N   ++ RE+ +R+D+
Sbjct:  496 IFVRNLSYDLTWQKLKEKFSHCGQVMFAEIKME-NGRSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRIDR 569          

HSP 3 Score: 57.3806 bits (137), Expect = 2.924e-8
Identity = 32/90 (35.56%), Postives = 50/90 (55.56%), Query Frame = 3
Query:  429 EKDGPITNSLFIANLDYTLTWQKLKDMFKN-AGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPA 695
            EK     N +FI+N+ Y + WQ +KD+ +   G V +V+L  DA+GKSR  G VEF+  E    A+   N   ++ R + ++ D  G+ A
Sbjct:   74 EKKSSHRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRVSGVVEFKDDEFVKKAVLTMNKHDLNGRPLNIKEDPDGEHA 163          

HSP 4 Score: 54.299 bits (129), Expect = 2.825e-7
Identity = 25/78 (32.05%), Postives = 43/78 (55.13%), Query Frame = 3
Query: 1377 ASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            A   + ++N+ +  TW+ LK  FS    +M  EI  ++  RSKG G VRF++   A +   +M+GT  +GR + +  +
Sbjct:  492 AGCQIFVRNLSYDLTWQKLKEKFSHCGQVMFAEIKMEN-GRSKGCGTVRFDSPESAEKACRLMNGTKINGREVDVRID 568          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Medaka
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:NCBI gene;Acc:101155179])

HSP 1 Score: 107.071 bits (266), Expect = 6.472e-24
Identity = 68/234 (29.06%), Postives = 120/234 (51.28%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVRE---------ETNKDR-----------------ERLSLSTPQHNFSSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ++NIP++   + LKD+ K+++G   ++ Y++      G + + F+T +  +KAV++++++ +NGR + V+E         E NK +                   +              P G + N+              I+ L+  G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE P EAV A+ +FN +++ NR + +++D+   P
Sbjct:   51 VSNIPYDVKWQTLKDLMKEKVG---EVTYVEHLMDAEGKSRVSFRTDELMKKAVEKVNRHVLNGRPLKVKEDPYGIISQREINKCQIGGPPVGPGGMGGMGGMGGMLGGMGGGMERMGPGPSGPMVNIPPSLMNNPNIPNEIIHGLQA-GRIGSTVFVANLDYKVGWKKLKEVFGMAGMVVRTDILEDKDGKSRGMGTVTFEMPIEAVQAVSMFNGQLLFNRTMHVKLDEKSLP 280          

HSP 2 Score: 67.0106 bits (162), Expect = 3.163e-11
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F   G V++ D+K + +GKS+G G V F++PE A  A    N   ++ REI +R+D+
Sbjct:  593 IFVRNLPFDFTWKNLKDTFNTCGMVQYADIKME-NGKSKGCGVVRFDSPETAERACRTMNGYRLNGREIDVRIDR 666          

HSP 3 Score: 58.151 bits (139), Expect = 1.984e-8
Identity = 31/77 (40.26%), Postives = 45/77 (58.44%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F      CG + Y D   +NGK  G  V+ F + + A++A + M+ Y +NGREI VR + N
Sbjct:  595 VRNLPFDFTWKNLKDTFN----TCGMVQYADIKMENGKSKGCGVVRFDSPETAERACRTMNGYRLNGREIDVRIDRN 667          

HSP 4 Score: 54.6842 bits (130), Expect = 2.319e-7
Identity = 29/97 (29.90%), Postives = 50/97 (51.55%), Query Frame = 3
Query: 1329 LHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            L  NP+ PN       A +   ++ + N+ +   WK LK  F  A  ++ T+I  D   +S+G+G V FE   +AV+  +M +G L   R +H++ +
Sbjct:  179 LMNNPNIPNEIIHGLQAGRIGSTVFVANLDYKVGWKKLKEVFGMAGMVVRTDILEDKDGKSRGMGTVTFEMPIEAVQAVSMFNGQLLFNRTMHVKLD 275          

HSP 5 Score: 53.9138 bits (128), Expect = 3.418e-7
Identity = 24/74 (32.43%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF +TWK+LK +F+    + + +I  ++  +SKG G+VRF++   A R    M+G   +GR I +  +
Sbjct:  593 IFVRNLPFDFTWKNLKDTFNTCGMVQYADIKMEN-GKSKGCGVVRFDSPETAERACRTMNGYRLNGREIDVRID 665          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Medaka
Match: hnrnpm (heterogeneous nuclear ribonucleoprotein M [Source:NCBI gene;Acc:101155179])

HSP 1 Score: 106.686 bits (265), Expect = 8.505e-24
Identity = 68/234 (29.06%), Postives = 120/234 (51.28%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVRE---------ETNKDR-----------------ERLSLSTPQHNFSSAGQPQGSLFNL--------------INELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKP 692
            ++NIP++   + LKD+ K+++G   ++ Y++      G + + F+T +  +KAV++++++ +NGR + V+E         E NK +                   +              P G + N+              I+ L+  G I +++F+ANLDY + W+KLK++F  AG V   D+  D DGKSRG GTV FE P EAV A+ +FN +++ NR + +++D+   P
Sbjct:   51 VSNIPYDVKWQTLKDLMKEKVG---EVTYVEHLMDAEGKSRVSFRTDELMKKAVEKVNRHVLNGRPLKVKEDPYGIISQREINKCQIGGPPVGPGGMGGMGGMGGMLGGMGGGMERMGPGPSGPMVNIPPSLMNNPNIPNEIIHGLQA-GRIGSTVFVANLDYKVGWKKLKEVFGMAGMVVRTDILEDKDGKSRGMGTVTFEMPIEAVQAVSMFNGQLLFNRTMHVKLDEKSLP 280          

HSP 2 Score: 67.0106 bits (162), Expect = 2.967e-11
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDK 680
            +F+ NL +  TW+ LKD F   G V++ D+K + +GKS+G G V F++PE A  A    N   ++ REI +R+D+
Sbjct:  581 IFVRNLPFDFTWKNLKDTFNTCGMVQYADIKME-NGKSKGCGVVRFDSPETAERACRTMNGYRLNGREIDVRIDR 654          

HSP 3 Score: 58.151 bits (139), Expect = 1.867e-8
Identity = 31/77 (40.26%), Postives = 45/77 (58.44%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYID---KNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETN 332
            + N+PF+ T K LKD F      CG + Y D   +NGK  G  V+ F + + A++A + M+ Y +NGREI VR + N
Sbjct:  583 VRNLPFDFTWKNLKDTFN----TCGMVQYADIKMENGKSKGCGVVRFDSPETAERACRTMNGYRLNGREIDVRIDRN 655          

HSP 4 Score: 54.6842 bits (130), Expect = 2.300e-7
Identity = 29/97 (29.90%), Postives = 50/97 (51.55%), Query Frame = 3
Query: 1329 LHPNPSRPNNDFLSDNASK---SLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            L  NP+ PN       A +   ++ + N+ +   WK LK  F  A  ++ T+I  D   +S+G+G V FE   +AV+  +M +G L   R +H++ +
Sbjct:  179 LMNNPNIPNEIIHGLQAGRIGSTVFVANLDYKVGWKKLKEVFGMAGMVVRTDILEDKDGKSRGMGTVTFEMPIEAVQAVSMFNGQLLFNRTMHVKLD 275          

HSP 5 Score: 54.299 bits (129), Expect = 2.878e-7
Identity = 24/74 (32.43%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 1389 LLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFN 1610
            + ++N+PF +TWK+LK +F+    + + +I  ++  +SKG G+VRF++   A R    M+G   +GR I +  +
Sbjct:  581 IFVRNLPFDFTWKNLKDTFNTCGMVQYADIKMEN-GKSKGCGVVRFDSPETAERACRTMNGYRLNGREIDVRID 653          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Medaka
Match: ncl (nucleolin [Source:NCBI gene;Acc:101158432])

HSP 1 Score: 64.6994 bits (156), Expect = 1.913e-10
Identity = 51/188 (27.13%), Postives = 89/188 (47.34%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRE--RLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKL 668
            + N+PF+ T+ +LK++F+D + +  +L    +NG   G A +EFK+   A+K ++E     + GR II+    +K ++  R+S S                          G +  +L + NL ++ T   L+  F+ A  +R        DG+ +GF  +EFE  E+A  A+  FN   +  R I+L
Sbjct:  409 VKNLPFSATVDDLKELFEDAVDV--RLPQ-GQNGSNRGIAYIEFKSEAVAEKMMEEAQGADVQGRSIILDFVGDKSQKGGRMSAS--------------------------GGVNKTLVVNNLAFSATEDVLQSTFEKAASIRI----PQRDGRPKGFAFIEFENAEDAKEALENFNNTEIEGRSIRL 563          
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Medaka
Match: pabpc4 (poly(A) binding protein cytoplasmic 4 [Source:NCBI gene;Acc:101159058])

HSP 1 Score: 63.1586 bits (152), Expect = 3.950e-10
Identity = 51/185 (27.57%), Postives = 84/185 (45.41%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIV-REETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            I N+  +   K L D F     I       D+NG   G A + F+T D A +A+++M+   +N R++ V R ++ K+RE                          EL        +++I N    +  ++LK+MF+  G+   V + TD+ GKSRGFG V FE  E+A  A+   N   ++ + +
Sbjct:  103 IKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGS-KGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKERE-------------------------AELGAKAKEFTNVYIKNFGDEMEDEQLKEMFEKYGKTLSVKVMTDSSGKSRGFGFVSFEKHEDANKAVEEINGTELNGKTV 261          
BLAST of Myelin expression factor 2 isoform 2 vs. Planmine SMEST
Match: SMESG000020746.1 (SMESG000020746.1)

HSP 1 Score: 980.704 bits (2534), Expect = 0.000e+0
Identity = 492/493 (99.80%), Postives = 492/493 (99.80%), Query Frame = 3
Query:  138 MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFKSNFDRNPGYGNTATSRGWNDAGGHXXXXXXXXXXXXXXXPYYRDRGVMDTKPQNSLSDFSNRIGRDYPSDSARDPRYNIGNSPSQASSSREMLLLEDILHKLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNNM 1616
            MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFKSNFDRNPGYGNTATSRGWNDAGGHSNLSSDSFFNQSFDNPYYRDRGVMDTKPQNSLSDFSNRIGRDYPSDSARDPRYNIGNSPSQ SSSREMLLLEDILHKLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNNM
Sbjct:    1 MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFKSNFDRNPGYGNTATSRGWNDAGGHSNLSSDSFFNQSFDNPYYRDRGVMDTKPQNSLSDFSNRIGRDYPSDSARDPRYNIGNSPSQGSSSREMLLLEDILHKLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNNM 493          
BLAST of Myelin expression factor 2 isoform 2 vs. Planmine SMEST
Match: SMESG000020746.1 (SMESG000020746.1)

HSP 1 Score: 976.467 bits (2523), Expect = 0.000e+0
Identity = 492/495 (99.39%), Postives = 492/495 (99.39%), Query Frame = 3
Query:  138 MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFKSNFDRNPGYGNTATSRGWNDAGGHXXXXXXXXXXXXXXXPYYRDRGVMDTKPQNSLSDFSNRIGRDYPSDSARDPRYNIG--NSPSQASSSREMLLLEDILHKLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNNM 1616
            MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFKSNFDRNPGYGNTATSRGWNDAGGHSNLSSDSFFNQSFDNPYYRDRGVMDTKPQNSLSDFSNRIGRDYPSDSARDPRYNIG  NSPSQ SSSREMLLLEDILHKLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNNM
Sbjct:    1 MKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRRVLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFKSNFDRNPGYGNTATSRGWNDAGGHSNLSSDSFFNQSFDNPYYRDRGVMDTKPQNSLSDFSNRIGRDYPSDSARDPRYNIGSNNSPSQGSSSREMLLLEDILHKLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDNASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRFETHGDAVRCKNMMDGTLFDGRPIHIEFNNM 495          
BLAST of Myelin expression factor 2 isoform 2 vs. Planmine SMEST
Match: SMESG000058745.1 (SMESG000058745.1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.202e-9
Identity = 48/187 (25.67%), Postives = 85/187 (45.45%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREE---TNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            I N+  + T+KE+ D F +   I       D+NG   G A + F     A+KA++     +ING+  ++RE+     K   R   STP  +F++                         +I N    L  + L+D+F+  G +    +  D +G S+GFG V F+  E+A+ A++  N +++ ++ I
Sbjct:  101 IKNLDPSITVKEINDTFANFGEISSCKVQTDENGVSKGFAFVNFNHEGDAEKAIQ-----AINGK--MIREKIVYVAKFIPRTQRSTPNKDFTNC------------------------YIKNFGIHLHDKSLRDLFEQCGEIVSAKVMVDKEGNSKGFGFVSFKEHEDAIKAVNTLNGRVIEDKTI 256          

HSP 2 Score: 51.6026 bits (122), Expect = 1.544e-6
Identity = 24/70 (34.29%), Postives = 40/70 (57.14%), Query Frame = 3
Query:  453 SLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            ++FI NLD ++T +++ D F N G +    ++TD +G S+GF  V F    +A  AI   N KM+  + +
Sbjct:   98 NIFIKNLDPSITVKEINDTFANFGEISSCKVQTDENGVSKGFAFVNFNHEGDAEKAIQAINGKMIREKIV 167          
BLAST of Myelin expression factor 2 isoform 2 vs. Planmine SMEST
Match: SMESG000004978.1 (SMESG000004978.1)

HSP 1 Score: 58.9214 bits (141), Expect = 8.077e-9
Identity = 49/182 (26.92%), Postives = 84/182 (46.15%), Query Frame = 3
Query:  141 KELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERL----SLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREIKLRM 674
            ++L+++F +   I      ID+NGK  G   + FK  + A+ AVK ++      R++ V     K+ ERL    S    Q    S+   QG                 +L++ NLD ++   +LK  F   G +    + TD++ +S+GFG V F  PEEA  A+   N K+  ++ + + +
Sbjct:  208 EKLREMFLEFGEITSAKVMIDENGKSKGFGFVCFKDSETAESAVKALNGKMFKDRQLYVGRAQKKN-ERLDELRSHFEKQRAERSSRYTQGV----------------NLYVKNLDDSIDDTRLKQEFVVFGNITSAKVMTDSNNRSKGFGFVCFSNPEEATKAVTEMNGKICGSKPLYVAL 372          

HSP 2 Score: 53.1434 bits (126), Expect = 4.888e-7
Identity = 47/184 (25.54%), Postives = 76/184 (41.30%), Query Frame = 3
Query:  111 ITNIPFNTTMKELKDVFKDRLGICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            I N+  + T KEL D F     I       D+  K  G   + F+T   A  A+  ++   I  +++ V               P+ N +++              + D   TN  +I N    L  +KL++MF   G +    +  D +GKS+GFG V F+  E A  A+   N KM  +R++
Sbjct:  107 IKNLDKSITQKELHDTFVAFGNILSCKIAADERSKSKGYGFVHFETEAAATNAIDAINGMLIKNKKVFVGR-----------FIPRTNRANS--------------DSDVKFTN-CYIKNFGEDLDDEKLREMFLEFGEITSAKVMIDENGKSKGFGFVCFKDSETAESAVKALNGKMFKDRQL 264          

HSP 3 Score: 52.7582 bits (125), Expect = 6.898e-7
Identity = 24/69 (34.78%), Postives = 38/69 (55.07%), Query Frame = 3
Query:  456 LFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIHLFNAKMVSNREI 662
            +FI NLD ++T ++L D F   G +    +  D   KS+G+G V FET   A  AI   N  ++ N+++
Sbjct:  105 IFIKNLDKSITQKELHDTFVAFGNILSCKIAADERSKSKGYGFVHFETEAAATNAIDAINGMLIKNKKV 173          
BLAST of Myelin expression factor 2 isoform 2 vs. Planmine SMEST
Match: SMESG000046155.1 (SMESG000046155.1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.360e-6
Identity = 42/151 (27.81%), Postives = 70/151 (46.36%), Query Frame = 3
Query:  189 LDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREETNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLDYTLTWQKLKDMFKNAGRVRHVDLKTDAD--GKSRGFGTVEFETPEEAVMAIHLFN 635
            L Y     K  G A +EF+  + AQ A+ +    SI+GR+  V   TN     +  S P                L+++L  +    N +++AN+   L+ + L+ +F+  G +    L+ D    GK R FG +E++TP+ A+ AI   N
Sbjct:  111 LSYDPITQKHMGYAFLEFEYPEAAQLALAQNTGISISGRQSKVGRPTN-----MPHSGP----------------LVDDLFAEANKYNRIYVANVHPNLSSEDLRLVFEAFGPIESCYLQPDVTRPGKHRAFGFIEYKTPQAAIEAIGAMN 240          
The following BLAST results are available for this feature:
BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HNRNPM1.447e-3135.38heterogeneous nuclear ribonucleoprotein M [Source:... [more]
HNRNPM2.640e-3035.07heterogeneous nuclear ribonucleoprotein M [Source:... [more]
MYEF25.989e-3034.60myelin expression factor 2 [Source:HGNC Symbol;Acc... [more]
MYEF26.914e-3034.60myelin expression factor 2 [Source:HGNC Symbol;Acc... [more]
HNRNPM8.566e-3035.38heterogeneous nuclear ribonucleoprotein M [Source:... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sup-461.287e-2029.41SUPpressor [Source:UniProtKB/TrEMBL;Acc:Q9XVS2][more]
sup-461.287e-2029.41SUPpressor [Source:UniProtKB/TrEMBL;Acc:Q9XVS2][more]
pab-11.325e-1126.97Polyadenylate-binding protein [Source:UniProtKB/T... [more]
pab-11.325e-1126.97Polyadenylate-binding protein [Source:UniProtKB/T... [more]
pab-11.325e-1126.97Polyadenylate-binding protein [Source:UniProtKB/T... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rump5.731e-2129.13gene:FBgn0267790 transcript:FBtr0082123[more]
rump5.700e-1236.59gene:FBgn0267790 transcript:FBtr0304684[more]
pAbp5.698e-723.81gene:FBgn0265297 transcript:FBtr0086738[more]
pAbp5.698e-723.81gene:FBgn0265297 transcript:FBtr0086736[more]
pAbp5.698e-723.81gene:FBgn0265297 transcript:FBtr0086740[more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myef21.424e-3133.97myelin expression factor 2 [Source:ZFIN;Acc:ZDB-GE... [more]
myef21.446e-3133.97myelin expression factor 2 [Source:ZFIN;Acc:ZDB-GE... [more]
hnrnpm1.481e-1945.88heterogeneous nuclear ribonucleoprotein M [Source:... [more]
hnrnpm4.929e-1845.88heterogeneous nuclear ribonucleoprotein M [Source:... [more]
hnrnpm4.980e-1845.88heterogeneous nuclear ribonucleoprotein M [Source:... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cep761.142e-2732.88centrosomal protein 76kDa [Source:Xenbase;Acc:XB-G... [more]
cep762.789e-2632.42centrosomal protein 76kDa [Source:Xenbase;Acc:XB-G... [more]
cep762.916e-2632.42centrosomal protein 76kDa [Source:Xenbase;Acc:XB-G... [more]
cep761.351e-2532.74centrosomal protein 76kDa [Source:Xenbase;Acc:XB-G... [more]
cep761.492e-2532.74centrosomal protein 76kDa [Source:Xenbase;Acc:XB-G... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Myef21.032e-3034.12myelin basic protein expression factor 2, represso... [more]
Gm98331.678e-3034.12predicted gene 9833 [Source:MGI Symbol;Acc:MGI:364... [more]
Myef23.862e-3034.12myelin basic protein expression factor 2, represso... [more]
Myef24.194e-3034.12myelin basic protein expression factor 2, represso... [more]
Myef24.199e-3034.12myelin basic protein expression factor 2, represso... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9P2K5|MYEF2_HUMAN3.029e-2934.60Myelin expression factor 2 OS=Homo sapiens OX=9606... [more]
sp|Q62826|HNRPM_RAT3.359e-2935.38Heterogeneous nuclear ribonucleoprotein M OS=Rattu... [more]
sp|Q8C854|MYEF2_MOUSE3.446e-2934.12Myelin expression factor 2 OS=Mus musculus OX=1009... [more]
sp|P52272|HNRPM_HUMAN5.470e-2835.07Heterogeneous nuclear ribonucleoprotein M OS=Homo ... [more]
sp|Q9D0E1|HNRPM_MOUSE1.835e-2734.60Heterogeneous nuclear ribonucleoprotein M OS=Mus m... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183PSS24.572e-4739.81Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A3Q0KEV89.468e-4638.83Rna recognition motif (Rrm) domain containing prot... [more]
Q86EB12.089e-4538.05Clone ZZD61 mRNA sequence OS=Schistosoma japonicum... [more]
Q5DC865.932e-4538.05SJCHGC01283 protein OS=Schistosoma japonicum OX=61... [more]
A0A183KQH11.170e-4439.32Uncharacterized protein OS=Schistosoma curassoni O... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myef23.857e-3234.06myelin expression factor 2 [Source:NCBI gene;Acc:1... [more]
hnrnpm6.680e-1744.71heterogeneous nuclear ribonucleoprotein M [Source:... [more]
hnrnpm6.758e-1744.71heterogeneous nuclear ribonucleoprotein M [Source:... [more]
ENSAMXT00000035946.13.332e-1025.84polyadenylate-binding protein 1-like [Source:NCBI ... [more]
ENSAMXT00000018970.28.150e-1024.64polyadenylate-binding protein 1-like [Source:NCBI ... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
myef24.455e-1737.63myelin expression factor 2 [Source:ZFIN;Acc:ZDB-GE... [more]
pabpc45.619e-1126.29poly(A) binding protein, cytoplasmic 4 (inducible ... [more]
pabpc45.991e-1126.26poly(A) binding protein, cytoplasmic 4 (inducible ... [more]
ncl9.823e-925.93nucleolin [Source:ZFIN;Acc:ZDB-GENE-030131-6986][more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
GBP22.687e-1728.88Poly(A+) RNA-binding protein; key surveillance fac... [more]
HRB11.567e-1325.64Poly(A+) RNA-binding protein; key surveillance fac... [more]
PAB11.068e-1026.46Poly(A) binding protein; part of the 3'-end RNA-pr... [more]
NOP133.017e-621.40Nucleolar protein found in preribosomal complexes;... [more]
SGN13.449e-625.58Cytoplasmic RNA-binding protein; contains an RNA r... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO455324.108e-3133.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO332061.097e-923.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO373426.998e-727.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO474879.279e-725.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO352431.133e-633.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
myef27.448e-3034.76myelin expression factor 2 [Source:NCBI gene;Acc:1... [more]
hnrnpm6.472e-2429.06heterogeneous nuclear ribonucleoprotein M [Source:... [more]
hnrnpm8.505e-2429.06heterogeneous nuclear ribonucleoprotein M [Source:... [more]
ncl1.913e-1027.13nucleolin [Source:NCBI gene;Acc:101158432][more]
pabpc43.950e-1027.57poly(A) binding protein cytoplasmic 4 [Source:NCBI... [more]
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BLAST of Myelin expression factor 2 isoform 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020746.10.000e+099.80SMESG000020746.1[more]
SMESG000020746.10.000e+099.39SMESG000020746.1[more]
SMESG000058745.11.202e-925.67SMESG000058745.1[more]
SMESG000004978.18.077e-926.92SMESG000004978.1[more]
SMESG000046155.11.360e-627.81SMESG000046155.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030516 ID=SMED30030516|Name=Myelin expression factor 2 isoform 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1770bp
TAAATTGATTTTATTTTGTTTTCAAAATGTCTAACAGGAATGTTAAAGAA
ACTCGTCGATCCAGATCCAGATCACCGTTGAAAAGTTCAGTTCGAAGTAG
ACGTTTACTAATTACAAATATTCCATTTAACACTACCATGAAAGAGCTAA
AAGATGTTTTTAAAGATAGACTTGGAATATGTGGAAAACTAGATTATATT
GATAAAAATGGAAAATTTTCTGGAAATGCTGTAATGGAATTCAAAACTGT
AGATGGAGCTCAAAAAGCCGTTAAAGAAATGCACAAATACTCAATCAATG
GCAGGGAAATCATAGTTCGTGAAGAAACGAACAAAGACCGTGAAAGGTTA
AGTTTAAGCACTCCACAACATAATTTCTCATCAGCTGGACAGCCTCAGGG
TTCACTATTTAATTTAATTAATGAATTAGAAAAGGATGGACCAATTACTA
ATTCACTATTTATTGCAAATCTTGACTATACTTTAACTTGGCAAAAGTTA
AAAGACATGTTTAAAAATGCTGGTAGAGTAAGACACGTAGATTTAAAGAC
TGATGCCGATGGAAAGTCTCGTGGTTTCGGTACTGTTGAGTTTGAAACTC
CAGAGGAAGCTGTAATGGCTATTCATCTTTTCAACGCTAAAATGGTGAGT
AATCGTGAAATTAAATTGCGCATGGATAAATCAGGGAAACCTGCTCTCGG
TAAATTAGATGTATCGAGATTTGGAAATATAGATTCAGGCCCTCAAAATA
ATACATTAAGCCCACAAGTTAGACGAGTTTTAGAAGATATTCTGGCTTCA
GTTACCAATCCTAATAGAAGCAGCCCTCAGGCAATGAAAGCGTTAAATGA
ATTGTTATCTTTGGCACAGATAATGGATAAAGGTCAAGGTCAACAATATC
CGATGCAAGATAATATCCCCTTTAAAAGTAATTTTGATAGAAATCCTGGA
TATGGTAATACTGCTACTTCCCGAGGTTGGAATGACGCTGGGGGTCATTC
CAATTTAAGTAGCGATTCATTTTTTAATCAGAGTTTTGATAATCCATATT
ACAGAGATAGAGGTGTAATGGATACAAAACCCCAAAATTCTTTAAGTGAT
TTTTCTAATAGAATCGGGCGCGATTATCCATCAGATTCTGCAAGAGATCC
TAGATATAATATAGGAAATTCACCAAGTCAGGCATCAAGTAGTCGTGAAA
TGCTATTACTTGAAGACATCTTACACAAACTAAAATCTTCGAACAACTTT
GATCAATTTGGCGTGTTGAATATTTTGTCCTCTGTCATGAATAAACGAAA
TGATGATCCATATTCTGGAATTAATCAGTTACATCCAAATCCTTCTAGAC
CTAATAATGACTTTCTATCCGATAATGCTTCGAAATCCCTGTTGATTAAA
AATATTCCATTTTCTTATACTTGGAAAGATCTGAAGCACAGTTTTTCACA
GGCTCCCGGTATAATGCATACCGAAATATTTCGAGATGACTTCAATAGAT
CAAAAGGAATTGGGTTGGTACGGTTTGAAACACACGGCGATGCGGTTCGT
TGTAAAAATATGATGGATGGAACATTGTTTGATGGGAGACCTATACACAT
TGAATTTAACAATATGTAGAAATAGTCATGGTCTATGAATTGCTCTTGCT
ATTGGTATTTTAAATCAATTCTATTCTTCGAACGTTACCATTTGTTTTTA
TCGCGCTCTGTTGTGTGGCAGTGATGATAATGGAAATCTTTTATTGTATC
ATTAAATGTGTATTTGTTTT
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protein sequence of SMED30030516-orf-1

>SMED30030516-orf-1 ID=SMED30030516-orf-1|Name=SMED30030516-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=531bp
MSNRNVKETRRSRSRSPLKSSVRSRRLLITNIPFNTTMKELKDVFKDRLG
ICGKLDYIDKNGKFSGNAVMEFKTVDGAQKAVKEMHKYSINGREIIVREE
TNKDRERLSLSTPQHNFSSAGQPQGSLFNLINELEKDGPITNSLFIANLD
YTLTWQKLKDMFKNAGRVRHVDLKTDADGKSRGFGTVEFETPEEAVMAIH
LFNAKMVSNREIKLRMDKSGKPALGKLDVSRFGNIDSGPQNNTLSPQVRR
VLEDILASVTNPNRSSPQAMKALNELLSLAQIMDKGQGQQYPMQDNIPFK
SNFDRNPGYGNTATSRGWNDAGGHSNLSSDSFFNQSFDNPYYRDRGVMDT
KPQNSLSDFSNRIGRDYPSDSARDPRYNIGNSPSQASSSREMLLLEDILH
KLKSSNNFDQFGVLNILSSVMNKRNDDPYSGINQLHPNPSRPNNDFLSDN
ASKSLLIKNIPFSYTWKDLKHSFSQAPGIMHTEIFRDDFNRSKGIGLVRF
ETHGDAVRCKNMMDGTLFDGRPIHIEFNNM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR035979RBD_domain_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 26..98
e-value: 2.8E-12
score: 56.8
coord: 143..215
e-value: 2.5E-21
score: 86.9
coord: 454..526
e-value: 2.1E-18
score: 77.1
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 144..213
e-value: 2.3E-18
score: 65.8
coord: 455..524
e-value: 5.8E-16
score: 58.1
coord: 27..95
e-value: 4.2E-11
score: 42.5
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 453..530
score: 13.976
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 25..102
score: 13.225
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 142..219
score: 17.387
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 1..113
e-value: 1.5E-15
score: 58.9
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 141..224
e-value: 1.3E-21
score: 78.4
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3DG3DSA:3.30.70.330coord: 417..530
e-value: 1.1E-18
score: 69.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 344..388
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 350..364
NoneNo IPR availablePANTHERPTHR23003RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEINcoord: 9..528
NoneNo IPR availableCDDcd00590RRM_SFcoord: 455..526
e-value: 1.78614E-18
score: 77.7305
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 17..105
IPR035979RNA-binding domain superfamilySUPERFAMILYSSF54928RNA-binding domain, RBDcoord: 142..527