DUF862 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030498 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15
Homology
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A164QG11 (DUF862 domain-containing protein OS=Daphnia magna OX=35525 GN=APZ42_028510 PE=4 SV=1) HSP 1 Score: 74.7146 bits (182), Expect = 3.425e-12 Identity = 39/118 (33.05%), Postives = 67/118 (56.78%), Query Frame = 1 Query: 7 LIYYRKMSVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDKLVAKIGGNERNST 360 ++Y +K+ +P A G R ++I ++ + +L+D +E I++V YK L SWQ+ L+HAF++FETN W+WS+EKN G+ IQ+SK D + + +R ++ Sbjct: 16 VVYTKKLYFDPNADSPEG----RDYWITDSELR------ETLSELVDVTERIIEVRYYKHPLGSWQLMDNVLHHAFIVFETNAWWWSIEKNDEGVTIQRSKDFDSVCHRYRRTKRKTS 123
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A164JSN2 (Uncharacterized protein OS=Daphnia magna OX=35525 GN=APZ42_000269 PE=4 SV=1) HSP 1 Score: 74.7146 bits (182), Expect = 4.467e-12 Identity = 42/103 (40.78%), Postives = 58/103 (56.31%), Query Frame = 1 Query: 25 MSVNP-KAQKKGGNWESRIHFINNTSAPNE---VFNGSEMRK----LIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSK 309 MS+ P + G N+ +++F P+ + E+R+ LIDT E I K Y L S Q T+ LYHAF++ ETNDW+WS+EKN GI IQ+SK Sbjct: 1 MSLLPSPSAPTGCNYTCKLYFDPKADQPSTGGYLITDEELRQKLANLIDTEERIQKASVYSNPLSSLQATNALLYHAFIVIETNDWWWSIEKNTEGITIQRSK 103
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A162DGJ0 (DUF862 domain-containing protein OS=Daphnia magna OX=35525 GN=APZ42_024281 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 1.076e-11 Identity = 41/102 (40.20%), Postives = 57/102 (55.88%), Query Frame = 1 Query: 25 MSVNPKAQKKGGNWESRIHFINNTSAPNEV---FNGSEMRK----LIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSK 309 +S++P A GGN+ +++F + ++R+ LIDT E I K Y L SWQ+ YHAFV+ ETNDW+WS+EKN GI IQ+SK Sbjct: 3 LSLSPSAST-GGNYTCKLYFDPGADQSDTRGYWITDDQLRQKLVNLIDTDEEIKKASIYSNPLSSWQLIDAMFYHAFVVMETNDWWWSIEKNTKGITIQRSK 103
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: E9GQZ6 (Uncharacterized protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_225508 PE=4 SV=1) HSP 1 Score: 67.781 bits (164), Expect = 1.079e-9 Identity = 31/58 (53.45%), Postives = 43/58 (74.14%), Query Frame = 1 Query: 136 KLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSK 309 KL DTSE I+KV Y L WQ+T + L HA+++FET++WYWS+EKN + + IQ+SK Sbjct: 53 KLTDTSEEILKVMYYTHPLNWWQVTKYNLQHAYIVFETDNWYWSIEKNDNCVAIQRSK 110
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A3M6TN79 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00005107 PE=4 SV=1) HSP 1 Score: 62.003 bits (149), Expect = 2.284e-7 Identity = 29/60 (48.33%), Postives = 39/60 (65.00%), Query Frame = 1 Query: 130 MRKLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSK 309 M LID SE I+ +W +K L WQ T +L H FV+ +T +W+WS+EKN I IQ+SK Sbjct: 65 MSLLIDFSENILNIWVFKSPLSDWQRTQLFLNHQFVVIKTKNWWWSIEKNSEVILIQRSK 124
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024663.1 (SMESG000024663.1) HSP 1 Score: 230.335 bits (586), Expect = 1.664e-75 Identity = 113/116 (97.41%), Postives = 114/116 (98.28%), Query Frame = 1 Query: 25 MSVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDKLVAKIGGNERNSTTLLE 372 MSVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLV WQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKD LVAKIGGNERNSTTLL+ Sbjct: 1 MSVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVWWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDNLVAKIGGNERNSTTLLD 116
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024661.1 (SMESG000024661.1) HSP 1 Score: 198.749 bits (504), Expect = 4.730e-63 Identity = 96/116 (82.76%), Postives = 107/116 (92.24%), Query Frame = 1 Query: 25 MSVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDKLVAKIGGNERNSTTLLE 372 MSVNP A+KKGGNW SRI FINNTSAPN+VF GSEMRKLIDTSETI++ WKYK+KLV +QITSF+LYHAFVIFET DWYWS+EKNGSGIWIQKSKSK++LV K GGNERNSTTLL+ Sbjct: 1 MSVNPDAEKKGGNWLSRIFFINNTSAPNDVFKGSEMRKLIDTSETIIQAWKYKQKLVWFQITSFFLYHAFVIFETKDWYWSIEKNGSGIWIQKSKSKNELVTKNGGNERNSTTLLD 116
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024649.1 (SMESG000024649.1) HSP 1 Score: 119.013 bits (297), Expect = 2.378e-32 Identity = 53/106 (50.00%), Postives = 79/106 (74.53%), Query Frame = 1 Query: 52 KGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDKLVAKIGGNERNSTTLL 369 KGG WES++ IN+ PN ++ GS+M+++ + SE I +VW+YKE L SWQ+TS YH F++ +T+ W WS+EKNG+GIW+QKS++ ++L+ K GG ER S +LL Sbjct: 6 KGGKWESQLFLINDIRHPNNLYYGSQMKQMGNLSERIQQVWRYKEPLKSWQLTSVIFYHIFLVLQTDKWNWSIEKNGTGIWVQKSENHNELLKKKGGVERKSVSLL 111
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024669.1 (SMESG000024669.1) HSP 1 Score: 118.627 bits (296), Expect = 3.306e-32 Identity = 51/104 (49.04%), Postives = 75/104 (72.12%), Query Frame = 1 Query: 61 NWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDKLVAKIGGNERNSTTLLE 372 +WE RI FI +T+ PNE++NG EM++ D S+ I+ VWKYK + Q+TSFY YH F++ ET++++WS+EKN GIWIQK + K+ +++K+ G ERN LE Sbjct: 7 DWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTSFYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLE 110
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000070711.1 (SMESG000070711.1) HSP 1 Score: 83.9593 bits (206), Expect = 3.482e-19 Identity = 41/111 (36.94%), Postives = 71/111 (63.96%), Query Frame = 1 Query: 52 KGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKL-----VSWQITSFYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDKLVAKIGGNERNSTTLL 369 KG NWE +I +I++ P E++N E+ + I + ETIV++ YK L V ++ ++HAFV+ +T ++Y+S +KNG GI+IQKS++KD ++ + G++R+ L Sbjct: 3 KGQNWEGKIFYIDDLERPEEIYNQHEILQKIGSKETIVEIDVYKTPLQGLDFVEEKVYEKIVHHAFVVVKTTNFYFSFDKNGRGIYIQKSENKDDVLKSVFGDQRSRPVKL 113 The following BLAST results are available for this feature:
BLAST of DUF862 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of DUF862 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DUF862 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030498 ID=SMED30030498|Name=DUF862 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1037bpback to top protein sequence of SMED30030498-orf-1 >SMED30030498-orf-1 ID=SMED30030498-orf-1|Name=SMED30030498-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp MIGIGQWRRTAAEFGSRNPRAKINLWLKLEAMKEILQLFSSSQRHFVTIKback to top protein sequence of SMED30030498-orf-2 >SMED30030498-orf-2 ID=SMED30030498-orf-2|Name=SMED30030498-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=125bp NLLIYYRKMSVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTback to top Annotated Terms
The following terms have been associated with this transcript:
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