GCR036
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030497 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of GCR036 vs. Ensembl Human
Match: HTR2B (5-hydroxytryptamine receptor 2B [Source:HGNC Symbol;Acc:HGNC:5294]) HSP 1 Score: 58.9214 bits (141), Expect = 7.893e-9 Identity = 93/385 (24.16%), Postives = 164/385 (42.60%), Query Frame = 2
Query: 71 QLSNIKISKMTLMTQDGSQV--AATLIMLSVICVIGSLGNGLVII-VYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWE--WGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPN-DLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSA---KSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNF-RTAAMLSVVALMYIISFLPAFLMINYP------------QILYIPLFYMYYINNAANPIIYCFMNARFRE 1159
Q +I ++ + G+++ AA LI++ +I IG GN LVI+ V K Q T F+ LA+ D + ++P+ + +E W + LC + F + S + I++DRYI I P + S+ + + +TV L+ I V IKGI + D N+ C LT + + F + +T I+++ ++ I+ +KK+ L +KN W + ++ V DE P P+ ML + K+ PNS + RR S Q N R + +L +V ++++ + P F + N Q+L ++ Y+++ NP++Y N FR+
Sbjct: 35 QTESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIG--GNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLP-LVLCPAWLFLDVLFSTASIMHLCAISVDRYIAIKKPIQA--NQYNSRATAFIKITVVWLISIGIAIPVP-IKGIETDVDNPNNITCV----LTKERFGDFMLFGSLAAF----FTPLAIMIVTYFLTIHA-----LQKKAYL--VKNKPPQRLTWLT---VSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGD-----ETLMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCP-FFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRD 389
BLAST of GCR036 vs. Ensembl Human
Match: FPR2 (formyl peptide receptor 2 [Source:HGNC Symbol;Acc:HGNC:3827]) HSP 1 Score: 55.0694 bits (131), Expect = 9.012e-9 Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 2
Query: 140 LIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTI----YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
L++L V V+G LGNGLVI V + + TT+ LA+ D F+ +P I E W +G FLCKL + + + S FLI IA+DR IC+ HP
Sbjct: 30 LVVLGVTFVLGVLGNGLVIWVAGFRMTRTVTTICYLNLALAD-FSFTATLPFLIVSMAMGEKWPFG---WFLCKLIHIVVDINLFGSVFLIGFIALDRCICVLHP 130
BLAST of GCR036 vs. Ensembl Human
Match: DRD2 (dopamine receptor D2 [Source:HGNC Symbol;Acc:HGNC:3023]) HSP 1 Score: 57.7658 bits (138), Expect = 1.473e-8 Identity = 94/421 (22.33%), Postives = 156/421 (37.05%), Query Frame = 2
Query: 134 ATLIMLSVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFF--SIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDT--------------------MLSSAKST--------PNSKSFLTVPKPEHR--------------------------------------RVSLRSICDTQIFQNFRTAA--MLSVVALMYIISFLPAFLM--------INYPQILYIPLFYMYYINNAANPIIYCFMNARFRE 1159
ATL+ L + ++ GN LV + +R K Q TT I LA+ D ++P +Y+E EW S C ++ + S + I+IDRY + P L+ ++ S +TV + + +F + C + L N E + + V ++S+ I+ + +Y+ IY VL+ RRK+ K + + N T+ D+ + + + MLSS P S LT+P P H R SL+++ ++ Q A ML++V ++II +LP F+ N P +LY ++ Y+N+A NPIIY N FR+
Sbjct: 38 ATLLTLLIAVIV--FGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVGEWKFSRIH-CDIFVTLDVMMCTASILNLCAISIDRYTAVAMPM-----LYNTRYSSKRRVTVMISIVWVLSFTI---------------SCPLLFGLNNADQNECIIANPAFVVYSSIVSFYVPFIVTLLVYIKIYI-VLRRRRKRVNTKRSSRAFRAHLRAPLKGNCTHPEDMKLCTVIMKSNGSFPVNRRRVEAARRAQELEMEMLSSTSPPERTRYSPIPPSHHQLTLPDPSHHGLHSTPDSPAKPEKNGHAKDHPKIAKIFEIQTMPNGKTRTSLKTMSRRKLSQQKEKKATQMLAIVLGVFIICWLPFFITHILNIHCDCNIPPVLYSAFTWLGYVNSAVNPIIYTTFNIEFRK 434
BLAST of GCR036 vs. Ensembl Human
Match: FPR2 (formyl peptide receptor 2 [Source:HGNC Symbol;Acc:HGNC:3827]) HSP 1 Score: 56.9954 bits (136), Expect = 2.071e-8 Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 2
Query: 140 LIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTI----YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
L++L V V+G LGNGLVI V + + TT+ LA+ D F+ +P I E W +G FLCKL + + + S FLI IA+DR IC+ HP
Sbjct: 30 LVVLGVTFVLGVLGNGLVIWVAGFRMTRTVTTICYLNLALAD-FSFTATLPFLIVSMAMGEKWPFG---WFLCKLIHIVVDINLFGSVFLIGFIALDRCICVLHP 130
BLAST of GCR036 vs. Ensembl Human
Match: FPR2 (formyl peptide receptor 2 [Source:HGNC Symbol;Acc:HGNC:3827]) HSP 1 Score: 56.9954 bits (136), Expect = 2.071e-8 Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 2
Query: 140 LIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTI----YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
L++L V V+G LGNGLVI V + + TT+ LA+ D F+ +P I E W +G FLCKL + + + S FLI IA+DR IC+ HP
Sbjct: 30 LVVLGVTFVLGVLGNGLVIWVAGFRMTRTVTTICYLNLALAD-FSFTATLPFLIVSMAMGEKWPFG---WFLCKLIHIVVDINLFGSVFLIGFIALDRCICVLHP 130
BLAST of GCR036 vs. Ensembl Celegans
Match: npr-10 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q6EUU1]) HSP 1 Score: 56.6102 bits (135), Expect = 1.198e-8 Identity = 36/101 (35.64%), Postives = 54/101 (53.47%), Query Frame = 2
Query: 152 SVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCI---TIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++I V+G GN LV++ R K Q +FI L+++D F I ++ P+T + + W +G LC L + FS ++ IAIDRYI ICHP
Sbjct: 36 TIIVVVGVTGNLLVVMSVMRFKVLQSVRNMFIVSLSVSDIFVAIVSGSVTPITAFSKVWLFG---GPLCHLLPLLQGTALSFSTLTLTAIAIDRYILICHP 133
BLAST of GCR036 vs. Ensembl Celegans
Match: npr-10 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q6EUU1]) HSP 1 Score: 56.6102 bits (135), Expect = 1.285e-8 Identity = 36/101 (35.64%), Postives = 54/101 (53.47%), Query Frame = 2
Query: 152 SVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCI---TIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++I V+G GN LV++ R K Q +FI L+++D F I ++ P+T + + W +G LC L + FS ++ IAIDRYI ICHP
Sbjct: 36 TIIVVVGVTGNLLVVMSVMRFKVLQSVRNMFIVSLSVSDIFVAIVSGSVTPITAFSKVWLFG---GPLCHLLPLLQGTALSFSTLTLTAIAIDRYILICHP 133
BLAST of GCR036 vs. Ensembl Celegans
Match: ser-1 (SERotonin/octopamine receptor family; Serotonin receptor [Source:UniProtKB/TrEMBL;Acc:O17470]) HSP 1 Score: 56.6102 bits (135), Expect = 1.761e-8 Identity = 34/110 (30.91%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 131 AATLIMLSVICVIGSLGNGLV-IIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIE----YWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPF 445
L +L V+C+IG +GN LV + + T + T F+ LA+ D C ++P++I +E W W +S +C LY + + S +S+I++DRY+ I P
Sbjct: 56 GVALFLLPVLCLIGLIGNFLVCVAIATDRRLHNVTNYFLFSLALADLLVCCIVMPLSIVVEVRHGVWTWSVS---MCLLYVYSDVFLCSASIVHMSVISLDRYLGISQPL 162
BLAST of GCR036 vs. Ensembl Celegans
Match: ser-1 (SERotonin/octopamine receptor family; Serotonin receptor [Source:UniProtKB/TrEMBL;Acc:O17470]) HSP 1 Score: 56.6102 bits (135), Expect = 1.767e-8 Identity = 34/110 (30.91%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 131 AATLIMLSVICVIGSLGNGLV-IIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIE----YWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPF 445
L +L V+C+IG +GN LV + + T + T F+ LA+ D C ++P++I +E W W +S +C LY + + S +S+I++DRY+ I P
Sbjct: 8 GVALFLLPVLCLIGLIGNFLVCVAIATDRRLHNVTNYFLFSLALADLLVCCIVMPLSIVVEVRHGVWTWSVS---MCLLYVYSDVFLCSASIVHMSVISLDRYLGISQPL 114
BLAST of GCR036 vs. Ensembl Celegans
Match: dop-3 (Dopamine receptor 3 [Source:UniProtKB/Swiss-Prot;Acc:Q6RYS9]) HSP 1 Score: 53.1434 bits (126), Expect = 2.100e-7 Identity = 37/128 (28.91%), Postives = 66/128 (51.56%), Query Frame = 2
Query: 68 HQLSNIKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVII-VYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEY--WEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
H +++I+ M ++ + VAA + L ++ + GN LVI+ V+ + Q T + I LA++D I ++ +Y E+ ++WG+ S F C +Y + S + I++DRYI I HP
Sbjct: 6 HHVTDIESPLMVVLWR----VAAG-VFLPLVPTMAVFGNVLVIMSVFRERSLQTVTNMLIVSLAVSDFMVAIGVMSFGVYYEWNDFKWGLGS-FFCHVYQALDVACSTASILNLLAISLDRYIAIGHP 127
BLAST of GCR036 vs. Ensembl Fly
Match: CCHa1-R (gene:FBgn0050106 transcript:FBtr0086893) HSP 1 Score: 66.2402 bits (160), Expect = 1.433e-11 Identity = 40/109 (36.70%), Postives = 63/109 (57.80%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVY--TRKHDQLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKF 454
I+ ++I V+G LGNG +I+V+ R+ + T +I LA+ D IT +P+ T+Y +EYW +G +FLC L F + +I S F ++ ++ DRY I P KF
Sbjct: 86 ILFALIFVVGVLGNGTLIVVFLSVRQMRNVPNT-YILSLALADLLVIITTVPLASTVYTVEYWPYG---SFLCSLSEFMKDVSIGVSVFTLTALSGDRYFAIVDPLRKF 190
BLAST of GCR036 vs. Ensembl Fly
Match: sNPF-R (gene:FBgn0036934 transcript:FBtr0332667) HSP 1 Score: 62.003 bits (149), Expect = 4.608e-10 Identity = 39/111 (35.14%), Postives = 57/111 (51.35%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD-QLTTTLFINILAITDCFTCITIIPMT---IYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
++ + + V+G GN LV V R Q T +FI LA++D C+ +P T ++ W +G S LC L F C+I S ++ IAIDRY I +PF+ M L
Sbjct: 68 VLYATVFVLGVFGNVLVCYVVLRNRAMQTVTNIFITNLALSDILLCVLAVPFTPLYTFMGRWAFGRS---LCHLVSFAQGCSIYISTLTLTSIAIDRYFVIIYPFHPRMKL 175
BLAST of GCR036 vs. Ensembl Fly
Match: sNPF-R (gene:FBgn0036934 transcript:FBtr0074871) HSP 1 Score: 62.003 bits (149), Expect = 4.608e-10 Identity = 39/111 (35.14%), Postives = 57/111 (51.35%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD-QLTTTLFINILAITDCFTCITIIPMT---IYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
++ + + V+G GN LV V R Q T +FI LA++D C+ +P T ++ W +G S LC L F C+I S ++ IAIDRY I +PF+ M L
Sbjct: 68 VLYATVFVLGVFGNVLVCYVVLRNRAMQTVTNIFITNLALSDILLCVLAVPFTPLYTFMGRWAFGRS---LCHLVSFAQGCSIYISTLTLTSIAIDRYFVIIYPFHPRMKL 175
BLAST of GCR036 vs. Ensembl Fly
Match: NPFR (gene:FBgn0037408 transcript:FBtr0308316) HSP 1 Score: 58.151 bits (139), Expect = 6.172e-9 Identity = 41/103 (39.81%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 146 MLSVICVIGSLGNGLVIIVYTRKHDQLTT-TLFINILAITDCFTCITIIPMT---IYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
M V+ V G+LGN LV+I RK T LFI LAI+D C+ +P+T I +YW +G S + LCK I S I+ IA DRY I +P
Sbjct: 92 MYGVLIVFGALGNTLVVIAVIRKPIMRTARNLFILNLAISDLLLCLVTMPLTLMEILSKYWPYG-SCSILCKTIAMLQALCIFVSTISITAIAFDRYQVIVYP 193
BLAST of GCR036 vs. Ensembl Fly
Match: NPFR (gene:FBgn0037408 transcript:FBtr0308317) HSP 1 Score: 58.151 bits (139), Expect = 6.185e-9 Identity = 41/103 (39.81%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 146 MLSVICVIGSLGNGLVIIVYTRKHDQLTT-TLFINILAITDCFTCITIIPMT---IYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
M V+ V G+LGN LV+I RK T LFI LAI+D C+ +P+T I +YW +G S + LCK I S I+ IA DRY I +P
Sbjct: 92 MYGVLIVFGALGNTLVVIAVIRKPIMRTARNLFILNLAISDLLLCLVTMPLTLMEILSKYWPYG-SCSILCKTIAMLQALCIFVSTISITAIAFDRYQVIVYP 193
BLAST of GCR036 vs. Ensembl Zebrafish
Match: adra1d (adrenoceptor alpha 1D [Source:ZFIN;Acc:ZDB-GENE-090312-203]) HSP 1 Score: 62.3882 bits (150), Expect = 3.491e-10 Identity = 89/372 (23.92%), Postives = 160/372 (43.01%), Query Frame = 2
Query: 74 LSNIKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVII-VYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMIN----YP-----QILYIPLFYMYYINNAANPIIYCFMNARFRE 1159
LSNI + +T D SQV I L++ + +GN LVI+ V KH Q T FI LAI D I ++P + +E + C ++ + S + +I+IDRYI + H K+ T+ + + +++ V V + S + K P+D I ++T LF S L S+ +++++ +Y+ +Y ++ R KS+ G+K + S R + ++P+ D+ SS +S L+V + R + A L++V M+I+ +LP F ++ +P +++ +F++ Y N+ NPIIY + F+
Sbjct: 19 LSNISNTTCEGITLD-SQVIGVGIFLAIFISVAIVGNILVILSVLCNKHLQTVTNFFIVNLAIADLLLSIIVLPFSASLEVLGCWVFGRVFCNIWAAVDVLCCTASILSLCVISIDRYIGVKHCL-KYPTIMTERKAGVILVLVWVSSMVISIGPLLGWKEPPPSD-------ESICRITEEPGY-ALFSS------LFSFYLPLMVILVMYIRVY--IVARRTTKSLEAGVKRERDKSMEVVLRIHCRSMPE-----------DSSTSSKTKNHPFRSSLSVRLMKFSREK-------------KAAKTLAIVVGMFILCWLPFFFVLPLGSFFPALKPSDMVFKVIFWLGYFNSCINPIIYPCSSKEFQR 348
BLAST of GCR036 vs. Ensembl Zebrafish
Match: galr2b (galanin receptor 2b [Source:ZFIN;Acc:ZDB-GENE-131121-48]) HSP 1 Score: 61.2326 bits (147), Expect = 6.560e-10 Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD--QLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R TT LFI L++ D F I +P TIY +E W +G +F+CK+ +FF N T+ S+F ++ +++DRY+ I +P
Sbjct: 31 VVFSLIFLLGTIGNSLVLAVLLRSGQVGYNTTNLFILNLSVADFFFIIFCVPFQATIYSLEGWVFG---SFMCKVVHFFINLTMYASSFTLAAVSVDRYLAIRYP 132
BLAST of GCR036 vs. Ensembl Zebrafish
Match: galr2b (galanin receptor 2b [Source:ZFIN;Acc:ZDB-GENE-131121-48]) HSP 1 Score: 61.2326 bits (147), Expect = 6.560e-10 Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD--QLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R TT LFI L++ D F I +P TIY +E W +G +F+CK+ +FF N T+ S+F ++ +++DRY+ I +P
Sbjct: 31 VVFSLIFLLGTIGNSLVLAVLLRSGQVGYNTTNLFILNLSVADFFFIIFCVPFQATIYSLEGWVFG---SFMCKVVHFFINLTMYASSFTLAAVSVDRYLAIRYP 132
BLAST of GCR036 vs. Ensembl Zebrafish
Match: si:ch73-160i9.1 (si:ch73-160i9.1 [Source:ZFIN;Acc:ZDB-GENE-141212-254]) HSP 1 Score: 57.3806 bits (137), Expect = 8.995e-9 Identity = 33/105 (31.43%), Postives = 58/105 (55.24%), Query Frame = 2
Query: 140 LIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTI---YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPF 445
++ +++ ++G+ GN +VI V T +++ LA+ D C+T I I + +YW +G+ FLCK F + S FL+++I++DR +CI HPF
Sbjct: 27 MVFYTIVILLGTTGNSVVIWVAGFHMKSNVTKVWLVNLAVADLIFCLTRISSLISKVFFDYWPFGV---FLCKFNGFIKYANMFCSVFLLAVISVDRALCIWHPF 128
BLAST of GCR036 vs. Ensembl Zebrafish
Match: si:ch73-160i9.3 (si:ch73-160i9.3 [Source:ZFIN;Acc:ZDB-GENE-141212-252]) HSP 1 Score: 55.0694 bits (131), Expect = 5.515e-8 Identity = 37/132 (28.03%), Postives = 69/132 (52.27%), Query Frame = 2
Query: 65 LHQLSNIKIS---KMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITD---CFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
LH S+I +S + + + D T+++ ++ + G GN +VI V + + +++ LA+ D CFT I + ++ +YW +G+ FLCK FF + S FL+++I++DR +C+ P
Sbjct: 7 LHTHSHIDVSTHHEKSTLDVDAIMENITIVLYTITIIFGISGNSVVIWVAGFRLKPNVSNVWLVNLAVADLIFCFTRIISLIKNLFFDYWPFGL---FLCKFNGFFKYANMFCSVFLLAVISVDRALCVWCP 135
BLAST of GCR036 vs. Ensembl Xenopus
Match: sp3 (Sp3 transcription factor [Source:Xenbase;Acc:XB-GENE-977158]) HSP 1 Score: 57.3806 bits (137), Expect = 1.863e-8 Identity = 38/105 (36.19%), Postives = 63/105 (60.00%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHDQL--TTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R + TT LFI L++ D I +P TIY +E W +G AF+CK +FF T+ S+F ++ +++DRY+ I +P
Sbjct: 31 VIFSLIFLLGTVGNSLVLAVLLRSGQRTHNTTNLFILNLSVADVSFIIFCVPFQATIYSLEDWVFG---AFMCKAVHFFIYLTMYASSFTLAAVSVDRYLAIRYP 132
BLAST of GCR036 vs. Ensembl Xenopus
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:NCBI gene;Acc:100498122]) HSP 1 Score: 56.9954 bits (136), Expect = 2.229e-8 Identity = 38/114 (33.33%), Postives = 64/114 (56.14%), Query Frame = 2
Query: 134 ATLIMLSVICVIGSLGNGLV-IIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIY---IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
A+ +++ ++C+ LGNG+V IV + KH + T LFI LAI+D I +P T+ I W +G + +CK+ ++ S F + IA+DR+ CI +PF + +T+
Sbjct: 47 ASYLLIFILCM---LGNGVVCFIVLSSKHMRTVTNLFILNLAISDLLVGIFCMPTTLLDNIIAGWPFGST---VCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTI 154
BLAST of GCR036 vs. Ensembl Xenopus
Match: sp3 (Sp3 transcription factor [Source:Xenbase;Acc:XB-GENE-977158]) HSP 1 Score: 56.9954 bits (136), Expect = 2.428e-8 Identity = 38/105 (36.19%), Postives = 63/105 (60.00%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHDQL--TTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R + TT LFI L++ D I +P TIY +E W +G AF+CK +FF T+ S+F ++ +++DRY+ I +P
Sbjct: 54 VIFSLIFLLGTVGNSLVLAVLLRSGQRTHNTTNLFILNLSVADVSFIIFCVPFQATIYSLEDWVFG---AFMCKAVHFFIYLTMYASSFTLAAVSVDRYLAIRYP 155
BLAST of GCR036 vs. Ensembl Xenopus
Match: lmod3 (leiomodin 3 [Source:Xenbase;Acc:XB-GENE-5737414]) HSP 1 Score: 54.6842 bits (130), Expect = 1.082e-7 Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 2
Query: 137 TLIMLSVICVIGSLGNGLVIIVYTRKHD---QLTTTLFINILAITDCFTCITIIPM--TIYI-EYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICH 439
TL++ +I +G LGN LVI V R + TT +FI L+I D + IP T+Y+ W G AF+CK ++F ++ S F +S +++DRY+ I H
Sbjct: 38 TLVVFGMIFALGVLGNSLVITVLARNKPGKPRSTTNIFILNLSIADLAYLLFCIPFQSTVYVLPTWVLG---AFICKFIHYFFTVSMLVSIFTLSAMSVDRYVAIVH 141
BLAST of GCR036 vs. Ensembl Xenopus
Match: lmod3 (leiomodin 3 [Source:Xenbase;Acc:XB-GENE-5737414]) HSP 1 Score: 54.6842 bits (130), Expect = 1.089e-7 Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 2
Query: 137 TLIMLSVICVIGSLGNGLVIIVYTRKHD---QLTTTLFINILAITDCFTCITIIPM--TIYI-EYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICH 439
TL++ +I +G LGN LVI V R + TT +FI L+I D + IP T+Y+ W G AF+CK ++F ++ S F +S +++DRY+ I H
Sbjct: 40 TLVVFGMIFALGVLGNSLVITVLARNKPGKPRSTTNIFILNLSIADLAYLLFCIPFQSTVYVLPTWVLG---AFICKFIHYFFTVSMLVSIFTLSAMSVDRYVAIVH 143
BLAST of GCR036 vs. Ensembl Mouse
Match: Prlhr (prolactin releasing hormone receptor [Source:MGI Symbol;Acc:MGI:2135956]) HSP 1 Score: 63.1586 bits (152), Expect = 1.748e-10 Identity = 35/116 (30.17%), Postives = 63/116 (54.31%), Query Frame = 2
Query: 125 QVAATLIML-SVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWG-ISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
Q+ ++ML S++ V+G +GN L+++V R + T I LA++D C +P+T+ + G + LC L +F T+ S F ++ IA+DRY+ + HP + ++L
Sbjct: 58 QLKGLIVMLYSIVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCAACVPLTLAYAFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIALDRYVVLVHPLRRRISL 173
BLAST of GCR036 vs. Ensembl Mouse
Match: Fpr1 (formyl peptide receptor 1 [Source:MGI Symbol;Acc:MGI:107443]) HSP 1 Score: 58.151 bits (139), Expect = 6.079e-9 Identity = 51/131 (38.93%), Postives = 67/131 (51.15%), Query Frame = 2
Query: 92 SKMTLMTQDGS--QVAATLIMLSVIC--------VIGSLGNGLVIIVYT-RKHDQLTTTLFINILAITD-CFTCIT--IIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
S + LM GS V+A I+L V V+G LGNGLVI V R +TT ++N LAI D CFT I + +W +G F+CK Y + + S FLI+LIA+DR IC+ HP
Sbjct: 12 SAVNLMNVSGSTQSVSAGYIVLDVFSYLIFAVTFVLGVLGNGLVIWVAGFRMKHTVTTISYLN-LAIADFCFTSTLPFYIASMVMGGHWPFG---WFMCKFIYTVIDINLFGSVFLIALIALDRCICVLHP 138
BLAST of GCR036 vs. Ensembl Mouse
Match: Fpr-rs7 (formyl peptide receptor, related sequence 7 [Source:MGI Symbol;Acc:MGI:2448177]) HSP 1 Score: 56.9954 bits (136), Expect = 1.437e-8 Identity = 40/104 (38.46%), Postives = 62/104 (59.62%), Query Frame = 2
Query: 140 LIMLSVICVIGSLGNGLVIIVYT-RKHDQLTTTLFINILAITDCFTCITIIPMTI--YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
+++LS+ ++G +GNGLVI V R +TT ++N LA++D F+ +T +P I + EW + FLCK + N + S FLI+ IA+DR IC+ HP
Sbjct: 30 VVVLSITFLLGVIGNGLVIYVAGFRMTHTVTTICYLN-LALSD-FSYMTSLPFQITSIVMNGEW-LFGWFLCKFVHMIINVNLFLSIFLITFIAMDRCICVLHP 130
BLAST of GCR036 vs. Ensembl Mouse
Match: Fpr2 (formyl peptide receptor 2 [Source:MGI Symbol;Acc:MGI:1278319]) HSP 1 Score: 56.9954 bits (136), Expect = 1.528e-8 Identity = 38/106 (35.85%), Postives = 59/106 (55.66%), Query Frame = 2
Query: 137 TLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTI----YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
+++++S+ +G LGNGLVI V + TT++ LA+ D F+ +P + E W +G FLCKL + + + S FLI+LIA+DR IC+ HP
Sbjct: 29 SMVVVSITFFLGVLGNGLVIWVAGFRMPHTVTTIWYLNLALAD-FSFTATLPFLLVEMAMKEKWPFG---WFLCKLVHIVVDVNLFGSVFLIALIALDRCICVLHP 130
BLAST of GCR036 vs. Ensembl Mouse
Match: Adra1b (adrenergic receptor, alpha 1b [Source:MGI Symbol;Acc:MGI:104774]) HSP 1 Score: 56.6102 bits (135), Expect = 2.523e-8 Identity = 84/373 (22.52%), Postives = 152/373 (40.75%), Query Frame = 2
Query: 80 NIKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVII-VYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFR---TAAMLSVVALMYIISFLPAFLM---------INYPQILYIPLFYMYYINNAANPIIYCFMNARFRE 1159
N S TL D ++ + ++L + +GN LVI+ V +H + T FI LAI D T++P + +E + + C ++ + S + I+IDRYI + + ++ TL + ++ +L+V VL + S + K PND EC TE+ F+++ + +ILV+ V I K + ++K + N K+ + S+ + E DT+ S+ N +S + V ++F+ R A L +V M+I+ +LP F+ + P ++ +F++ Y N+ NPIIY + F+
Sbjct: 29 NQTSSNSTLPQLDVTRAISVGLVLGAFILFAIVGNILVILSVACNRHLRTPTNYFIVNLAIADLLLSFTVLPFSATLEVLGYWVLGRIFCDIWAAVDVLCCTASILSLCAISIDRYIGVRYSL-QYPTLVTRRKAILALLSVWVLSTVISIGPLLGWKEPAPND---DKECG---------VTEEPFYALFSSLGSFYIPLAVILVMYCRVYIVAKRTTKNLEAGVMKEMSNSKELTLRIHSK-------NFHE--------DTLSSTKAKGHNPRSSIAV----------------KLFKFSREKKAAKTLGIVVGMFILCWLPFFIALPLGSLFSTLKPPDAVFKVVFWLGYFNSCLNPIIYPCSSKEFKR 357
BLAST of GCR036 vs. UniProt/SwissProt
Match: sp|A1ZAX0|CCH1R_DROME (Neuropeptide CCHamide-1 receptor OS=Drosophila melanogaster OX=7227 GN=CCHa1-R PE=2 SV=2) HSP 1 Score: 66.2402 bits (160), Expect = 1.436e-10 Identity = 40/109 (36.70%), Postives = 63/109 (57.80%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVY--TRKHDQLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKF 454
I+ ++I V+G LGNG +I+V+ R+ + T +I LA+ D IT +P+ T+Y +EYW +G +FLC L F + +I S F ++ ++ DRY I P KF
Sbjct: 86 ILFALIFVVGVLGNGTLIVVFLSVRQMRNVPNT-YILSLALADLLVIITTVPLASTVYTVEYWPYG---SFLCSLSEFMKDVSIGVSVFTLTALSGDRYFAIVDPLRKF 190
BLAST of GCR036 vs. UniProt/SwissProt
Match: sp|Q64121|PRLHR_RAT (Prolactin-releasing peptide receptor OS=Rattus norvegicus OX=10116 GN=Prlhr PE=2 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 9.163e-10 Identity = 35/116 (30.17%), Postives = 63/116 (54.31%), Query Frame = 2
Query: 125 QVAATLIML-SVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWG-ISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
Q+ ++ML S++ V+G +GN L+++V R + T I LA++D C +P+T+ + G + LC L +F T+ S F ++ IA+DRY+ + HP + ++L
Sbjct: 58 QLKGLIVMLYSIVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCAACVPLTLAYAFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISL 173
BLAST of GCR036 vs. UniProt/SwissProt
Match: sp|Q6VMN6|PRLHR_MOUSE (Prolactin-releasing peptide receptor OS=Mus musculus OX=10090 GN=Prlhr PE=2 SV=2) HSP 1 Score: 63.1586 bits (152), Expect = 1.223e-9 Identity = 35/116 (30.17%), Postives = 63/116 (54.31%), Query Frame = 2
Query: 125 QVAATLIML-SVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWG-ISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
Q+ ++ML S++ V+G +GN L+++V R + T I LA++D C +P+T+ + G + LC L +F T+ S F ++ IA+DRY+ + HP + ++L
Sbjct: 58 QLKGLIVMLYSIVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCAACVPLTLAYAFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIALDRYVVLVHPLRRRISL 173
BLAST of GCR036 vs. UniProt/SwissProt
Match: sp|Q4EW11|PRLHR_BOVIN (Prolactin-releasing peptide receptor OS=Bos taurus OX=9913 GN=PRLHR PE=3 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 3.981e-9 Identity = 32/110 (29.09%), Postives = 60/110 (54.55%), Query Frame = 2
Query: 140 LIMLSVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWG-ISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
+++ S++ V+G +GN L+++V R + T I LA++D C +P+T+ + G + LC L +F T+ S F ++ IA+DRY+ + HP + ++L
Sbjct: 64 VLLYSIVVVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCTACVPLTLAYAFEPRGWVFGGGLCHLVFFLQPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISL 173
BLAST of GCR036 vs. UniProt/SwissProt
Match: sp|E7F7V7|GAL2B_DANRE (Galanin receptor 2b OS=Danio rerio OX=7955 GN=galr2b PE=2 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 4.952e-9 Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD--QLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R TT LFI L++ D F I +P TIY +E W +G +F+CK+ +FF N T+ S+F ++ +++DRY+ I +P
Sbjct: 31 VVFSLIFLLGTIGNSLVLAVLLRSGQVGYNTTNLFILNLSVADFFFIIFCVPFQATIYSLEGWVFG---SFMCKVVHFFINLTMYASSFTLAAVSVDRYLAIRYP 132
BLAST of GCR036 vs. TrEMBL
Match: A0A193KUG2 (GCR036 OS=Schmidtea mediterranea OX=79327 GN=gcr036 PE=2 SV=1) HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0 Identity = 365/366 (99.73%), Postives = 366/366 (100.00%), Query Frame = 2
Query: 83 IKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE 1180
I+ISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKVSVGLVLTVSVLLGIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE
Sbjct: 7 IQISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKVSVGLVLTVSVLLGIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE 372
BLAST of GCR036 vs. TrEMBL
Match: A0A193KUN5 (GCR056 OS=Schmidtea mediterranea OX=79327 GN=gcr056 PE=2 SV=1) HSP 1 Score: 276.559 bits (706), Expect = 1.661e-86 Identity = 172/366 (46.99%), Postives = 234/366 (63.93%), Query Frame = 2
Query: 98 MTLMTQD-GSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNS----YECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLW----ESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSL-RSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDI 1165
M+ MT++ +Q+ T+ +L +I VIGS+GN LV+I+Y++K D+ T FI LAI+DC C+ IIP TIYIE+ +W +S +CK YYF NN +IPF A LI+LIAIDR+ C+C+PF MT+ +SKV V +V +SV+LG+ S FQVK K + NS EC D+S+ N T E LFF+IVQ+ QL+SY CI +VICLY+ IY VL RKK+ LKG+ + S+LW +SRS D + + +P T + +T P P + ++ R +T + QNF+TA ML VVA+ YI++FLPA LM+N L +P+FYMYYINNAANPIIYCFMN FRED+
Sbjct: 1 MSTMTKELTNQIPTTIGILIIISVIGSIGNLLVLIIYSQKRDKSTANQFIKTLAISDCIVCLVIIPFTIYIEFNDWKVSIDMICKSYYFLNNSSIPFGALLITLIAIDRFFCVCYPFLSLMTVKRSKVCVFIVFMMSVVLGVLSVFQVKTFKLSKNSTNMNSTLIQSECIDVSRYANPTKYELLFFTIVQRSQLISYAICICVVICLYILIYRFVLAAARKKARLKGLNSSSTTSFLWNKFFKSRSIKRINSDSNNDDTSVPCQTT-----------EELMTKPGPSNNDKAIKRKFKETILIQNFKTALMLFVVAITYIVTFLPACLMVNSSLALNLPVFYMYYINNAANPIIYCFMNKNFREDV 355
BLAST of GCR036 vs. TrEMBL
Match: A0A0R3UIP1 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7314 PE=3 SV=1) HSP 1 Score: 272.707 bits (696), Expect = 2.609e-84 Identity = 164/419 (39.14%), Postives = 231/419 (55.13%), Query Frame = 2
Query: 98 MTLMTQD-GSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIK-NVKKPSYLWESRSNLTNVPDLDENPI---------------------------------------KLPDGDTMLSSAKSTPNSKSFLTVP-------------------KPE---HRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDI 1165
MTLMT G Q+ AT+IML + C IGS+GN +VI++Y KHD++T+T+FI LA+ D F C+ ++P+TIYIE EW I S FLCK YY NN IPFS+ LIS IA DRY ICHPF+K +T+ ++K +G++L ++++LG S + + + N YEC + +++ NTT +K + +VQ Q+ SY CI+ V+ LYV I+ VL R++++ L G K ++ P S S N P ++ NP+ +L + +S SKS VP KPE RR+SLR + + QN +TA ML VVAL+YII+ +PA LM +Y+P+FYMYY+NNA NPIIYCFMN FRED+
Sbjct: 1 MTLMTSTLGEQIPATMIMLGIFCSIGSIGNTIVIVIYATKHDRMTSTVFILALAMVDSFACVCLMPLTIYIESVEWRIQSEFLCKFYYVINNSFIPFSSLLISGIAFDRYFAICHPFSKIITVTRAKQIIGVMLALALVLGGVSAMTIVVEQNTASNATAPEYECTE-TRMLNTTEAQKAIYHVVQGSQISSYVLCILSVVILYVLIFRTVLIMRQRRTRLIGNKLRIQTPF----SDSGSPNKPKIESNPVEPLSGISHSNVQATNSMKQQAEEHNELLEQNVVSEIWKNRLKSNEKKQTSVDILDESKSIRPVPEKGAFSFKKKHKKRSSATSKPEGQQSRRISLRGLKGNAVLQNMKTAGMLFVVALVYIIALIPAMLMAIGVIDIYLPIFYMYYVNNAINPIIYCFMNPNFREDV 414
BLAST of GCR036 vs. TrEMBL
Match: A0A5K3EX19 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1) HSP 1 Score: 268.855 bits (686), Expect = 1.050e-82 Identity = 159/411 (38.69%), Postives = 226/411 (54.99%), Query Frame = 2
Query: 119 GSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIK-NVKKPSYLWESRSNLTNVPDLDENPI---------------------------------------KLPDGDTMLSSAKSTPNSKSFLTVP-------------------KPE---HRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDI 1165
G Q+ AT+IML + C IGS+GN +VI++Y KHD++T+T+FI LA+ D F C+ ++P+TIYIE EW I S FLCK YY NN IPFS+ LIS IA DRY ICHPF+K +T+ ++K +G++L ++++LG S + + + N YEC + +++ NTT +K + +VQ Q+ SY CI+ V+ LYV I+ VL R++++ L G K ++ P S S N P ++ NP+ +L + +S SKS VP KPE RR+SLR + + QN +TA ML VVAL+YII+ +PA LM +Y+P+FYMYY+NNA NPIIYCFMN FRED+
Sbjct: 6 GEQIPATMIMLGIFCSIGSIGNTIVIVIYATKHDRMTSTVFILALAMVDSFACVCLMPLTIYIESVEWRIQSEFLCKFYYVINNSFIPFSSLLISGIAFDRYFAICHPFSKIITVTRAKQIIGVMLALALVLGGVSAMTIVVEQNTASNATAPEYECTE-TRMLNTTEAQKAIYHVVQGSQISSYVLCILSVVILYVLIFRTVLIMRQRRTRLIGNKLRIQTPF----SDSGSPNKPKIESNPVEPLSGISHSNVQATNSMKQQAEEHNELLEQNVVSEIWKNRLKSNEKKQTSVDILDESKSIRPVPEKGAFSFKKKHKKRSSATSKPEGQQSRRISLRGLKGNAVLQNMKTAGMLFVVALVYIIALIPAMLMAIGVIDIYLPIFYMYYVNNAINPIIYCFMNPNFREDV 411
BLAST of GCR036 vs. TrEMBL
Match: A0A193KUC3 (GCR110 OS=Schmidtea mediterranea OX=79327 GN=gcr110 PE=2 SV=1) HSP 1 Score: 261.922 bits (668), Expect = 1.764e-80 Identity = 165/383 (43.08%), Postives = 236/383 (61.62%), Query Frame = 2
Query: 113 QDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGI-HPNDLT--NSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVK---------------KPSYLWESRSN--------LTNVPDLDEN--PIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRV--SLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKA 1171
Q Q+ TL +LS+I V+G +GN VI++Y R+ D++T+ LF+ +LA +D FTCI IIP TI+IE+ EWGI S+F+CK Y FFNN IPFSA LISLIA+DRY CICHPF + M L +SK+ + + L +S LLG ++FQV + K + + +D+ N C + S+ N + ++KLF+ I QK QL+SY+ CI++VI LY IY V++ RRKK LKG+ +K K Y E+ N ++N P + N P+ P + S++ + + S V +L+++ DT + QN + AAML VV + YI++FLPA LM N L +P+FY+YY+NNAANPIIYCFMN FR +K+
Sbjct: 2 QSTQQIPPTLALLSIISVVGGIGNLFVILIYIRQCDKVTSNLFMTVLAGSDIFTCIFIIPTTIFIEFHEWGIGSSFVCKSYMFFNNSLIPFSALLISLIAMDRYFCICHPFLQIMNLSRSKLFICIALMLSTLLGTLASFQVHIEKKLTNLSDIGPMNMSFCVEASEWANRSMSQKLFYQITQKSQLVSYSICILIVIGLYSTIYYTVIKVRRKKDQLKGMNKMKRFKPKKLECKSHIKPKSQYRLENSDNSPIYSRFVVSNYPGCNRNVKPLGNPSTNHHHSNSPTNSSMNSLNNSVNNSSSIVIKTLKNVRDTTLLQNLKLAAMLFVVGITYIVTFLPANLMANGIIKLNLPVFYLYYVNNAANPIIYCFMNRNFRNKLKS 384
BLAST of GCR036 vs. Ensembl Cavefish
Match: galr2b (galanin receptor type 2-like [Source:NCBI gene;Acc:103040671]) HSP 1 Score: 60.8474 bits (146), Expect = 7.613e-10 Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD--QLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R TT LFI L++ D F I +P TIY +E W +G +F+CK+ +FF N T+ S+F ++ +++DRY+ I +P
Sbjct: 31 VVFSLIFLLGTVGNSLVLAVLLRSGQVGYNTTNLFILNLSVADFFFIIFCVPFQATIYSLEGWVFG---SFMCKVVHFFINLTMYASSFTLAAVSVDRYLAIRYP 132
BLAST of GCR036 vs. Ensembl Cavefish
Match: npffr2b (neuropeptide FF receptor 2-like [Source:NCBI gene;Acc:103031865]) HSP 1 Score: 59.6918 bits (143), Expect = 2.140e-9 Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 2
Query: 68 HQLSNIKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLV-IIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIY---IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
HQL I+ + + S A ++ +I V+ +GNGLV IV K + T LFI LAI+D I +P T+ I W +G + +CKL ++ S F + +IA+DR+ CI +PF + +T+
Sbjct: 34 HQLPGPNITYVDFYLHEPSVSAIFIVSYLLIFVVCMVGNGLVCFIVLRSKSMRTVTNLFILNLAISDLLVGIFCMPTTLVDNIITGWPFG---SLVCKLSGMVQGISVSASVFTLVVIAVDRFRCIVYPFKQKLTI 166
BLAST of GCR036 vs. Ensembl Cavefish
Match: npffr2b (neuropeptide FF receptor 2-like [Source:NCBI gene;Acc:103031865]) HSP 1 Score: 55.0694 bits (131), Expect = 5.794e-8 Identity = 38/111 (34.23%), Postives = 61/111 (54.95%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLV-IIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIY---IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
+++ V+C++G NGLV IV K + T LFI LAI+D I +P T+ I W +G + +CKL ++ S F + +IA+DR+ CI +PF + +T+
Sbjct: 56 LLIFVVCMVG---NGLVCFIVLRSKSMRTVTNLFILNLAISDLLVGIFCMPTTLVDNIITGWPFG---SLVCKLSGMVQGISVSASVFTLVVIAVDRFRCIVYPFKQKLTI 160
BLAST of GCR036 vs. Ensembl Cavefish
Match: galr1a (galanin receptor type 1 [Source:NCBI gene;Acc:103037936]) HSP 1 Score: 53.9138 bits (128), Expect = 1.038e-7 Identity = 37/107 (34.58%), Postives = 59/107 (55.14%), Query Frame = 2
Query: 137 TLIMLSVICVIGSLGNGLVIIVYTRK---HDQLTTTLFINILAITDCFTCITIIPM--TIYI-EYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICH 439
TL++ +I +G LGN LVI V R+ + TT +FI L+I D + IP T+Y+ W +G F+CK ++F ++ S F +S +++DRY+ I H
Sbjct: 28 TLLIFGLIFTLGVLGNSLVITVLARRKSGQQRSTTNVFILNLSIADLSYLLFCIPFQSTVYMMPSWIFG---EFICKFVHYFFTVSMLVSIFTLSAMSVDRYVAIVH 131
BLAST of GCR036 vs. Ensembl Cavefish
Match: GALR1 (galanin receptor type 1 [Source:NCBI gene;Acc:103047815]) HSP 1 Score: 53.1434 bits (126), Expect = 2.259e-7 Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 2
Query: 92 SKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHD---QLTTTLFINILAITDCFTCITIIPM--TIYI-EYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICI 433
S L + G++ TL++ ++I +G LGN +VI V R + TT +FI L++ D + +P T+Y+ W G AF CK ++F ++ S F +S +++DRY+ I
Sbjct: 10 SGTQLALEIGAENVITLLVFALIFALGVLGNAVVITVLARSRPGRARSTTNIFILNLSVADLSYLLFCVPFQATVYVLPTWVLG---AFACKFVHYFFTVSMLVSIFTLSAMSVDRYVAI 126
BLAST of GCR036 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011352.1 (pep scaffold:Pmarinus_7.0:GL478211:42083:43114:1 gene:ENSPMAG00000010325.1 transcript:ENSPMAT00000011352.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 59.6918 bits (143), Expect = 3.668e-10 Identity = 46/143 (32.17%), Postives = 76/143 (53.15%), Query Frame = 2
Query: 20 SSTIFLSAQMNGKCILHQLSNIKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEY--WEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
+ST ++S+Q L L+NI + + D + A ++I+ +V+ V+GS+GN LVI + K + TLF L+I D F + IP+ I W W + +A +CKL F + S + ++LI++DRY + HP
Sbjct: 5 NSTTWVSSQTT----LAWLTNITDDEAPKTSNDTVKYA-SIIVFTVVFVLGSVGNFLVIWILGFKMKRTMVTLFFLHLSIAD-FILVMQIPVVIAHGAMNWHWAMGTA-MCKLISFGTFVNMNASVYFLTLISLDRYAALVHP 140
BLAST of GCR036 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010994.1 (pep scaffold:Pmarinus_7.0:GL482718:13396:14511:-1 gene:ENSPMAG00000009967.1 transcript:ENSPMAT00000010994.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 55.8398 bits (133), Expect = 6.178e-9 Identity = 48/151 (31.79%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 14 FSSSTIFLSAQMNGKCILHQLSNIKISKMTLMTQDGSQVAATL-----IMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPM---TIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
F+++T++ S N Q ++SK T T + S TL +L ++ V+GS+GN LVI + K + TLF L+I D F + IP+ T+Y+E +W + +A CKL +F + S + ++LI++DRY + HP
Sbjct: 9 FNTTTVWNSVAWNTS----QTIVTRLSKSTDKTPETSTSTDTLKYSAITILIIVFVLGSIGNFLVIWILGFKMKRTVVTLFFLHLSIAD-FIFVMQIPLVIVTVYMEA-QWVLGAA-ACKLVWFTLAVNMNASVYFLTLISLDRYAALLHP 152
BLAST of GCR036 vs. Ensembl Sea Lamprey
Match: prlh2r (prolactin releasing hormone 2 receptor [Source:ZFIN;Acc:ZDB-GENE-120411-41]) HSP 1 Score: 52.7582 bits (125), Expect = 6.056e-8 Identity = 35/122 (28.69%), Postives = 61/122 (50.00%), Query Frame = 2
Query: 119 GSQVAATL-----IMLSVICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTI-YIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTL 463
G Q+ TL + + + V+G LGN L++ V R K T L I LA +D C +P+T+ Y+ + +F+C + ++ S F +++IA+DRYI +P + +T+
Sbjct: 13 GVQLIRTLKPFVVLFYTAVVVVGVLGNFLLVYVICRVKKMHNVTNLLIGNLAFSDLLMCAMCVPLTLAYVFDARGWVFGSFMCYFVFLMQPVSVYVSVFTLTVIAVDRYIVTVYPLRRRITI 134
BLAST of GCR036 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007473.1 (pep scaffold:Pmarinus_7.0:GL476896:142244:145503:-1 gene:ENSPMAG00000006752.1 transcript:ENSPMAT00000007473.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.8322 bits (120), Expect = 2.006e-7 Identity = 31/101 (30.69%), Postives = 62/101 (61.39%), Query Frame = 2
Query: 152 SVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIY--IEYWEWGISSAFLCKLY--YFFNNCTIPFSAFLISLIAIDRYICICHP 442
++I +G+ NGL +++ +RK + +T++++ LA+ D F + +P IY I+ W + + LC++Y +FF N F+ ++++I++DRY+ I HP
Sbjct: 7 TIIVALGAPLNGLAVVMLSRK-KKTSTSIYLLTLALVDLFYLL-FLPFKIYYNIQMNNWTLPT-ILCRMYVIFFFTNTY--FTLIMLAVISVDRYLAIVHP 102
BLAST of GCR036 vs. Ensembl Sea Lamprey
Match: si:dkey-202l22.3 (prolactin releasing hormone receptor [Source:HGNC Symbol;Acc:HGNC:4464]) HSP 1 Score: 50.447 bits (119), Expect = 3.539e-7 Identity = 32/127 (25.20%), Postives = 60/127 (47.24%), Query Frame = 2
Query: 107 MTQDGSQVAATLIMLSV----------ICVIGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWG-ISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNK 451
++Q+ L++LS+ + ++G GN L+I+V R K T + LA++D C+T +P++ + G + A LC L T+ S ++ IA+DRY+ + +P +
Sbjct: 50 LSQEQEHFEGFLVLLSLKQIFIPLYALLTLVGVSGNLLLILVIARTKRLHNATNFLLGNLALSDLLVCLTCVPISAAFAFEPRGWVYGALLCYLVALLQPATVHVSVLSLTAIALDRYVVVAYPIRR 176
BLAST of GCR036 vs. Ensembl Nematostella
Match: EDO37023 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SH02]) HSP 1 Score: 55.4546 bits (132), Expect = 1.033e-8 Identity = 85/361 (23.55%), Postives = 138/361 (38.23%), Query Frame = 2
Query: 146 MLSVICV---IGSLGNGLVIIVYTR-KHDQLTTTLFINILAITDCFTCITIIPMTIYIEYW---EWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQ-ILY----------IPLFYMYYINNAANPIIYCFMNARFREDIKAI 1174
M S +C+ IG +GN LV+ V TR K + FI LAI+D P+ I I ++ ++ + CK+ F S L+ I I+R+ I HP N MT + + ++ + S F +++ + ++ T Y CA I T LF S+ Y +++++ LY I C V W+ D +NP++ S+SF +V K + V M V L++ ++++P L + + ILY + L+ + N+ NP IY F N RFR+ KAI
Sbjct: 1 MTSCLCIAALIGFVGNLLVLAVLTRTKKFRTVMNRFIVHLAISDLLVSSICTPLFIKINFFFDSDYDVK----CKISRFLQYLAPEVSMVLLITIGINRHRAIVHPLN-IMTYKTANKLIASAWLYALAVVTPSIFLTQVLHHTNLDNTTVRY-CATIQTSTQAGRAYVLFLSV------FGYVIPLVVLVALYARISCVV----------------------WKR--------DTSKNPLR---------------TSRSFESVLKTRKKVVK-----------------MFLTVILLFFVTWIPLLLYVGVIEGILYKTARRLDEVRMVLYAIGLSNSVCNPFIYSFFNNRFRDGCKAI 287
BLAST of GCR036 vs. Ensembl Nematostella
Match: EDO45025 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU57]) HSP 1 Score: 55.8398 bits (133), Expect = 1.089e-8 Identity = 60/235 (25.53%), Postives = 107/235 (45.53%), Query Frame = 2
Query: 68 HQLSNIKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVI-IVYT-RKHDQLTTT-LFINILAITDCFTCITIIPMTIYI--EYWEWGISS-AFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSI 754
L N +S + + + G + A+L++L +IG GNGLV+ ++Y+ R Q + LF+ LA++D C+ +P+TI++ Y E SS CK+ F S +++ I+IDR+ + P + +L S+ L V + TF ++ + N + CA I + LF S+ ++ ++ +I LYV + V +RK S+
Sbjct: 7 ETLENQTVSTLAIWSTSGGEFIASLLILGAFSLIGLAGNGLVVFVIYSARSRGQKSAVQLFLLHLAVSDLMVCLLCVPLTIFVNFSYSEHHSSSDDVTCKVARFIQFLAPTSSICILTAISIDRFYSLVKPLKR-KSLLTRPFSLLLSAWVYAGVLFLPTFYFTKVEQLLTNK-GKVWYCATIPNNVTSGVIYLLFLSVT------AFVIPLVTMIVLYVRVGKTVWSRQRKISL 233
BLAST of GCR036 vs. Ensembl Nematostella
Match: EDO39181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAU0]) HSP 1 Score: 52.373 bits (124), Expect = 9.788e-8 Identity = 40/123 (32.52%), Postives = 63/123 (51.22%), Query Frame = 2
Query: 131 AATLIMLSVICVIGSLGNGLVI-IVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIY--IEYWEW-----GISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSK 475
A L+ +V+ ++ GN L I +V + H + TT +FI LAI+D I +P +++ + + GI AFLCKL+ F +I S + I DR++ I +PF +TL SK
Sbjct: 1 AIKLLAYNVVFIVAFFGNILAISVVLSNVHLKTTTNVFILNLAISDLLIAIVCMPSSMHSIVAHSRQTSEVSGIFGAFLCKLHQFLQGISITSSILTVECIGCDRFLAIVYPFKNIITLPISK 123
BLAST of GCR036 vs. Ensembl Nematostella
Match: EDO50021 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF31]) HSP 1 Score: 52.373 bits (124), Expect = 1.023e-7 Identity = 33/96 (34.38%), Postives = 50/96 (52.08%), Query Frame = 2
Query: 158 ICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMT-IYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
IC+IG+LGN LV++ ++ + T FI LA+ D C+ IP++ +Y W S +LCK + T+ S + IA DR+I I P
Sbjct: 6 ICIIGALGNLLVMLATRKRRMRNVTNQFIANLALADFTVCVITIPISLVYTNLGHWPFRS-WLCKTMPYLQGITVSASVGTLVAIASDRFIVIVFP 100
BLAST of GCR036 vs. Ensembl Nematostella
Match: EDO44425 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVQ0]) HSP 1 Score: 52.373 bits (124), Expect = 1.559e-7 Identity = 35/106 (33.02%), Postives = 56/106 (52.83%), Query Frame = 2
Query: 149 LSVICVIGSLGNGLVIIVYTRKHDQLTTT--LFINILAITDCFTCITIIPMTIY------IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
L +I VI LGN LV+ + RK Q+ LF+ +AI+D F + ++P +Y I + G A LCKL +F ++ + S ++ LIAI+R+ + P
Sbjct: 35 LGLILVIALLGNSLVLAL-VRKSSQMNGAINLFVANMAISDLFVPLFVLPREMYQLTSGTISWQVRGTGGAILCKLVFFASDISPIVSVLMLCLIAIERFTAVVFP 139
BLAST of GCR036 vs. Ensembl Medaka
Match: galr2a (galanin receptor type 2 [Source:NCBI gene;Acc:101155603]) HSP 1 Score: 60.8474 bits (146), Expect = 7.799e-10 Identity = 39/105 (37.14%), Postives = 65/105 (61.90%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLVIIVYTRKHD--QLTTTLFINILAITDCFTCITIIPM--TIY-IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++ S+I ++G++GN LV+ V R TT LFI L++ D F I +P TIY +E W +G +F+CK+ +FF N T+ S+F ++ +++DRY+ I +P
Sbjct: 31 VVFSLIFLLGTIGNSLVLAVLLRSGQVGYNTTNLFILNLSVADFFFIIFCVPFQATIYSLEGWVFG---SFMCKVVHFFINLTMYASSFTLAAVSVDRYLAIRYP 132
BLAST of GCR036 vs. Ensembl Medaka
Match: si:ch73-160i9.1 (C3a anaphylatoxin chemotactic receptor [Source:NCBI gene;Acc:101158210]) HSP 1 Score: 59.3066 bits (142), Expect = 2.169e-9 Identity = 35/105 (33.33%), Postives = 60/105 (57.14%), Query Frame = 2
Query: 137 TLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITD---CFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++I+ ++ V+G GN +VI V K T +++ LAI D CFT + + ++ ++W +G+ FLCK FF + S FL+++I++DR +CI HP
Sbjct: 32 SIILYTLTVVLGITGNSVVIWVAGFKLKPKVTNVWLVNLAIADLIFCFTRVFSLIKKLFFDHWPFGV---FLCKFNGFFKYANMFCSVFLLAVISLDRMLCIWHP 133
BLAST of GCR036 vs. Ensembl Medaka
Match: si:ch73-160i9.1 (C3a anaphylatoxin chemotactic receptor [Source:NCBI gene;Acc:101158210]) HSP 1 Score: 58.9214 bits (141), Expect = 2.623e-9 Identity = 35/105 (33.33%), Postives = 60/105 (57.14%), Query Frame = 2
Query: 137 TLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITD---CFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHP 442
++I+ ++ V+G GN +VI V K T +++ LAI D CFT + + ++ ++W +G+ FLCK FF + S FL+++I++DR +CI HP
Sbjct: 46 SIILYTLTVVLGITGNSVVIWVAGFKLKPKVTNVWLVNLAIADLIFCFTRVFSLIKKLFFDHWPFGV---FLCKFNGFFKYANMFCSVFLLAVISLDRMLCIWHP 147
BLAST of GCR036 vs. Ensembl Medaka
Match: htr2b (5-hydroxytryptamine receptor 2B [Source:NCBI gene;Acc:101162203]) HSP 1 Score: 58.151 bits (139), Expect = 6.059e-9 Identity = 86/368 (23.37%), Postives = 153/368 (41.58%), Query Frame = 2
Query: 131 AATLIMLSVICVIGSLGNGLVII-VYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGIS-SAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHP-NDLT--NSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKK-------PSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNF-RTAAMLSVVALMYIISFLPAFLMINYPQIL-----------YIPLF-YMYYINNAANPIIYCFMNARFRE 1159
AA LI++ +I IG GN LVI+ V + Q T F+ LA+ D + ++P+ + + G FLC ++ F + S + I++DRYI I P ++K V + L + + IA +K + HP N++T +++ C L T T + + + + + IY +Q RKK L K ++ + E+ + + +D KLP L+ TP ++ L+ RR+S Q N R + +L +V L++++ + P F + N L + +F ++ Y+++ NP++Y N FRE
Sbjct: 27 AALLIIMVIIPTIG--GNILVILAVSLERKLQNATNYFLMSLAVADLLVGLLVMPIALVTVLYNSGWPLPDFLCPIWLFLDVLFSTASIMHLCAISLDRYIAIKKPIQHSQYKSRAKAIVKIALVWLISICIAIPIPIKGLTNYHPSNNITFNSNHTC-----LLKTDTFRDFI--------IFGSLAAFFIPLMIMMVIYLLTVQVLRKKVYLLKSKVTQRFNSQIVSSVFQREAATPPLQMEPMDMLDTKLPRMQDKLNEGMGTPPTEDELS-----FRRMSTMGKKSMQTLSNEQRASKVLGIVFLLFVVMWCP-FFITNITSALCTSCSASIVSRLMEIFVWVGYVSSGINPLVYTLFNKTFRE 373
BLAST of GCR036 vs. Ensembl Medaka
Match: npffr2b (neuropeptide FF receptor 2-like [Source:NCBI gene;Acc:101174760]) HSP 1 Score: 56.225 bits (134), Expect = 3.058e-8 Identity = 39/115 (33.91%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 143 IMLSVICVIGSLGNGLV-IIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIY---IEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSK 475
+++ V+C++G NG+V IV K+ + T LFI LAI+D I +P T+ I W +G + +CKL ++ S F + IA+DR+ CI +PF + +T+ SK
Sbjct: 53 LLIFVVCMVG---NGVVCFIVLRSKNMRTVTNLFILNLAISDLLVGIFCMPTTLLDNIITGWPFG---SIVCKLSGMVQGISVSASVFTLVAIAVDRFRCIVYPFRQKLTIGTSK 161
BLAST of GCR036 vs. Planmine SMEST
Match: SMESG000064403.1 (SMESG000064403.1) HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0 Identity = 365/366 (99.73%), Postives = 366/366 (100.00%), Query Frame = 2
Query: 83 IKISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE 1180
I+ISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKVSVGLVLTVSVLLGIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE
Sbjct: 7 IQISKMTLMTQDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKVSVGLVLTVSVLLGIASTFQVKMIKGIHPNDLTNSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE 372
BLAST of GCR036 vs. Planmine SMEST
Match: SMESG000030128.1 (SMESG000030128.1) HSP 1 Score: 276.559 bits (706), Expect = 4.618e-90 Identity = 172/366 (46.99%), Postives = 234/366 (63.93%), Query Frame = 2
Query: 98 MTLMTQD-GSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNS----YECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKPSYLW----ESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSL-RSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDI 1165
M+ MT++ +Q+ T+ +L +I VIGS+GN LV+I+Y++K D+ T FI LAI+DC C+ IIP TIYIE+ +W +S +CK YYF NN +IPF A LI+LIAIDR+ C+C+PF MT+ +SKV V +V +SV+LG+ S FQVK K + NS EC D+S+ N T E LFF+IVQ+ QL+SY CI +VICLY+ IY VL RKK+ LKG+ + S+LW +SRS D + + +P T + +T P P + ++ R +T + QNF+TA ML VVA+ YI++FLPA LM+N L +P+FYMYYINNAANPIIYCFMN FRED+
Sbjct: 1 MSTMTKELTNQIPTTIGILIIISVIGSIGNLLVLIIYSQKRDKSTANQFIKTLAISDCIVCLVIIPFTIYIEFNDWKVSIDMICKSYYFLNNSSIPFGALLITLIAIDRFFCVCYPFLSLMTVKRSKVCVFIVFMMSVVLGVLSVFQVKTFKLSKNSTNMNSTLIQSECIDVSRYANPTKYELLFFTIVQRSQLISYAICICVVICLYILIYRFVLAAARKKARLKGLNSSSTTSFLWNKFFKSRSIKRINSDSNNDDTSVPCQTT-----------EELMTKPGPSNNDKAIKRKFKETILIQNFKTALMLFVVAITYIVTFLPACLMVNSSLALNLPVFYMYYINNAANPIIYCFMNKNFREDV 355
BLAST of GCR036 vs. Planmine SMEST
Match: SMESG000078415.1 (SMESG000078415.1) HSP 1 Score: 261.922 bits (668), Expect = 4.905e-84 Identity = 165/383 (43.08%), Postives = 236/383 (61.62%), Query Frame = 2
Query: 113 QDGSQVAATLIMLSVICVIGSLGNGLVIIVYTRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGI-HPNDLT--NSYECADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVK---------------KPSYLWESRSN--------LTNVPDLDEN--PIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRV--SLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKA 1171
Q Q+ TL +LS+I V+G +GN VI++Y R+ D++T+ LF+ +LA +D FTCI IIP TI+IE+ EWGI S+F+CK Y FFNN IPFSA LISLIA+DRY CICHPF + M L +SK+ + + L +S LLG ++FQV + K + + +D+ N C + S+ N + ++KLF+ I QK QL+SY+ CI++VI LY IY V++ RRKK LKG+ +K K Y E+ N ++N P + N P+ P + S++ + + S V +L+++ DT + QN + AAML VV + YI++FLPA LM N L +P+FY+YY+NNAANPIIYCFMN FR +K+
Sbjct: 2 QSTQQIPPTLALLSIISVVGGIGNLFVILIYIRQCDKVTSNLFMTVLAGSDIFTCIFIIPTTIFIEFHEWGIGSSFVCKSYMFFNNSLIPFSALLISLIAMDRYFCICHPFLQIMNLSRSKLFICIALMLSTLLGTLASFQVHIEKKLTNLSDIGPMNMSFCVEASEWANRSMSQKLFYQITQKSQLVSYSICILIVIGLYSTIYYTVIKVRRKKDQLKGMNKMKRFKPKKLECKSHIKPKSQYRLENSDNSPIYSRFVVSNYPGCNRNVKPLGNPSTNHHHSNSPTNSSMNSLNNSVNNSSSIVIKTLKNVRDTTLLQNLKLAAMLFVVGITYIVTFLPANLMANGIIKLNLPVFYLYYVNNAANPIIYCFMNRNFRNKLKS 384
BLAST of GCR036 vs. Planmine SMEST
Match: SMESG000037778.1 (SMESG000037778.1) HSP 1 Score: 206.068 bits (523), Expect = 1.620e-62 Identity = 136/373 (36.46%), Postives = 213/373 (57.10%), Query Frame = 2
Query: 125 QVAATLIMLSVICVIGSLGNGLVIIVY-TRKHDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIAST-FQVKMIKGIHPN---DLTNSYECADISKLT-NTTTTEKL-FFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTRRKKSILKGIKNVKKP---SYLWESRSNLTNVPDL-------DENPIKLPD--GDTMLSSAKSTPNSKSFLTVP--KPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILYIPLFYMYYINNAANPIIYCFMNARFREDIKAIVE 1180
Q+ +I + V IG +GNG+VI++Y ++++ T +LFI +LA+ D TC+ +IP T+ E+ W I S LC +YY N +PFS+ L S +AIDRY ICHPF++ T+ ++K+ + + S+ L I +T F + + N + Y+C D S+LT +T + L F I+ KI Y CII+VI LY+ IY ++ R +K L+G + K S R + +P++ +EN +K+P + ++S+ S++F + + R +S I D+ + QN R+A ML VVA++YI SF+PA LM N L + +FY+YY+NNAANPIIYCFMN +FR D K +++
Sbjct: 8 QIPIAMITMCVNSFIGCVGNGIVILIYCVQRNENCTVSLFIFVLAVIDFSTCLCVIPFTVVNEFLLWYIGSPALCFIYYVLNTFNVPFSSLLTSCVAIDRYCSICHPFHQIFTVHRAKILIISLGLFSLFLAITTTQFAFLSMAELASNISQGNKSEYKCTDPSQLTFHTVKSAHLKMFYILLKINHSCYVFCIIVVILLYILIYRAIILLRYRKRQLQGRIDAKSHEMMSSFMAIRKSEVAIPNISLVGLNPNENYLKIPQIKSGSFACYSRSSCCSRNFSVNDNFQTQIRSISKSLIKDSDLLQNIRSAIMLFVVAIVYITSFIPALLMANKMISLNLAVFYLYYVNNAANPIIYCFMNQQFRADFKRLMK 380
BLAST of GCR036 vs. Planmine SMEST
Match: SMESG000078101.1 (SMESG000078101.1) HSP 1 Score: 172.555 bits (436), Expect = 9.247e-50 Identity = 118/358 (32.96%), Postives = 200/358 (55.87%), Query Frame = 2
Query: 122 SQVAATLIMLSVICVIGSLGNGLVIIVYTRK----HDQLTTTLFINILAITDCFTCITIIPMTIYIEYWEWGISSAFLCKLYYFFNNCTIPFSAFLISLIAIDRYICICHPFNKFMTLFKSKXXXXXXXXXXXXXXIASTFQVKMIKGIHPNDLTNSYE-CADISKLTNTTTTEKLFFSIVQKIQLLSYTTCIILVICLYVAIYCKVLQTR-RKKSILKGIKNVKKPSYLWESRSNLTNVPDLDENPIKLPDGDTMLSSAKSTPNSKSFLTVPKPEHRRVSLRSICDTQIFQNFRTAAMLSVVALMYIISFLPAFLMINYPQILY-IPLFYMYYINNAANPIIYCFMNARFREDIKAI 1174
QV AT+++L+++ +G++GN LV++VY RK + T FIN LA++D C+ I+P T+ E++ + IS F CKLYYF N + FS LI+LIA+DR +CI P K +T+ K+K+ + ++L +S++LGI V IH + + E C +I N +++ + ++I + II VI Y +Y ++++ R R+K++L + + S T D+ + S A S+P + LT R+ + + D+ +F+N ++AA+L VVA++YI+ F P L+I + + + + L+ +YY+NNAANPI+YCFMN+ FR D+ +
Sbjct: 16 EQVIATIVILTIVVFVGTIGNILVLLVYYRKRGSEQNNETDCFFINSLALSDLIVCLVIVPCTMSTEWYNFHISLYF-CKLYYFMNTANLLFSTMLIALIAVDRCLCITLPLRKILTVNKAKIMMLILLLLSIILGIMGATLVGQNPHIHNSTVPQKTEVCEEIKFHKNVINEVLIYYEVYERITHSVFILSIIAVIICYALVYVEIIKIRKREKTLL----------FKHKRESKKTKSVDMSTS-----------SEAYSSPGEIALLT-----PRKSLAQGLRDSALFRNIKSAAILFVVAVVYILVFTPVILIITFKVVKFNVILYNLYYLNNAANPIVYCFMNSNFRRDLSKL 346 The following BLAST results are available for this feature:
BLAST of GCR036 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of GCR036 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of GCR036 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of GCR036 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of GCR036 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of GCR036 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of GCR036 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of GCR036 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030497 ID=SMED30030497|Name=GCR036|organism=Schmidtea mediterranea sexual|type=transcript|length=1180bpback to top protein sequence of SMED30030497-orf-1 >SMED30030497-orf-1 ID=SMED30030497-orf-1|Name=SMED30030497-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=393bp TEITFSSSTIFLSAQMNGKCILHQLSNIKISKMTLMTQDGSQVAATLIMLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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