Iron-sulfur cluster assembly scaffold protein IscU

Overview
NameIron-sulfur cluster assembly scaffold protein IscU
Smed IDSMED30030485
Length (bp)677
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Iron-sulfur cluster assembly scaffold protein IscU (SMED30030485) t-SNE clustered cells

Violin plots show distribution of expression levels for Iron-sulfur cluster assembly scaffold protein IscU (SMED30030485) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Iron-sulfur cluster assembly scaffold protein IscU (SMED30030485) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Iron-sulfur cluster assembly scaffold protein IscU (SMED30030485) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030485

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30030485SMESG000041819.1 dd_Smed_v4_1867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030485SMESG000041819.1 dd_Smed_v4_1867_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Human
Match: ISCU (iron-sulfur cluster assembly enzyme [Source:HGNC Symbol;Acc:HGNC:29882])

HSP 1 Score: 219.164 bits (557), Expect = 1.877e-72
Identity = 107/153 (69.93%), Postives = 119/153 (77.78%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K +  K
Sbjct:    9 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKK 161
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Human
Match: ISCU (iron-sulfur cluster assembly enzyme [Source:HGNC Symbol;Acc:HGNC:29882])

HSP 1 Score: 208.379 bits (529), Expect = 1.365e-68
Identity = 99/124 (79.84%), Postives = 109/124 (87.90%), Query Frame = 1 
Query:  172 KVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            +V+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K +  K
Sbjct:   13 QVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKK 136
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Human
Match: ISCU (iron-sulfur cluster assembly enzyme [Source:HGNC Symbol;Acc:HGNC:29882])

HSP 1 Score: 190.274 bits (482), Expect = 2.860e-61
Identity = 92/131 (70.23%), Postives = 102/131 (77.86%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCS 477
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCS
Sbjct:    9 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCS 139
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Human
Match: ISCU (iron-sulfur cluster assembly enzyme [Source:HGNC Symbol;Acc:HGNC:29882])

HSP 1 Score: 189.889 bits (481), Expect = 3.299e-61
Identity = 92/131 (70.23%), Postives = 102/131 (77.86%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCS 477
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCS
Sbjct:    9 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCS 139
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Human
Match: ISCU (iron-sulfur cluster assembly enzyme [Source:HGNC Symbol;Acc:HGNC:29882])

HSP 1 Score: 189.889 bits (481), Expect = 3.299e-61
Identity = 92/131 (70.23%), Postives = 102/131 (77.86%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCS 477
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCS
Sbjct:    9 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCS 139
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Celegans
Match: Y45F10D.4 (pep chromosome:WBcel235:IV:13783962:13784680:1 gene:WBGene00012885.1 transcript:Y45F10D.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y45F10D.4)

HSP 1 Score: 213.001 bits (541), Expect = 1.151e-70
Identity = 99/123 (80.49%), Postives = 110/123 (89.43%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKN 531
            YHEKV+DH+ENPRNVGS DKN+  VGTG+VGAPACGDVMKLQI+VD+NGKII+AKFKTFGCGSAIASSSLATEWI GKTI  A +IKN  IAKEL LPPVKLHCSMLA+DAI+AAL D+ KK 
Sbjct:   27 YHEKVIDHYENPRNVGSLDKNDPSVGTGIVGAPACGDVMKLQIRVDDNGKIIEAKFKTFGCGSAIASSSLATEWINGKTIDYASKIKNDEIAKELCLPPVKLHCSMLAQDAIQAALKDYQKKQ 149
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Fly
Match: IscU (gene:FBgn0037637 transcript:FBtr0081930)

HSP 1 Score: 221.09 bits (562), Expect = 9.743e-74
Identity = 108/142 (76.06%), Postives = 121/142 (85.21%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKN 531
            LRS+ L+ V + P      YHE V++H+ENPRNVGS DK +  VGTGLVGAPACGDVMKLQIKVDENGKI+DAKFKTFGCGSAIASSSLATEW+KGK+I EAG++KNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K 
Sbjct:   11 LRSQ-LKRVQSVP---VALYHENVVEHYENPRNVGSLDKKDVTVGTGLVGAPACGDVMKLQIKVDENGKIVDAKFKTFGCGSAIASSSLATEWVKGKSIDEAGKLKNTDIAKELRLPPVKLHCSMLAEDAIKAALADYKVKQ 148
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Zebrafish
Match: ISCU (si:ch211-191d15.2 [Source:ZFIN;Acc:ZDB-GENE-040724-113])

HSP 1 Score: 222.631 bits (566), Expect = 4.647e-74
Identity = 106/127 (83.46%), Postives = 115/127 (90.55%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            YH+KV+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQI+VDENGKIIDA+FKTFGCGSAIASSSLATEW+KGK+I EA +IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K  D K
Sbjct:   31 YHKKVVDHYENPRNVGSLDKNAKNVGTGLVGAPACGDVMKLQIQVDENGKIIDARFKTFGCGSAIASSSLATEWVKGKSIDEALKIKNTEIAKELCLPPVKLHCSMLAEDAIKAALADYRLKQKDEK 157
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Zebrafish
Match: iscub (iron-sulfur cluster assembly enzyme b [Source:ZFIN;Acc:ZDB-GENE-050417-332])

HSP 1 Score: 220.32 bits (560), Expect = 3.215e-73
Identity = 106/147 (72.11%), Postives = 124/147 (84.35%), Query Frame = 1 
Query:   94 IMQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            I+++L + TLR  S         YH+KV+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQ++VD+ GKI++AKFKTFGCGSAIASSSLATEW+KGK++ EA +IKNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K +
Sbjct:   14 ILKTLSAPTLRTQSG--------YHKKVVDHYENPRNVGSLDKNAKNVGTGLVGAPACGDVMKLQVEVDDAGKIVNAKFKTFGCGSAIASSSLATEWVKGKSVDEALKIKNTEIAKELSLPPVKLHCSMLAEDAIKAALADYRLKQE 152
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Zebrafish
Match: iscub (iron-sulfur cluster assembly enzyme b [Source:ZFIN;Acc:ZDB-GENE-050417-332])

HSP 1 Score: 218.779 bits (556), Expect = 1.518e-72
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 1 
Query:  136 NSPKLITNF-YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            ++P L T   YH+KV+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQ++VDE GKI++AKFKTFGCGSAIASSSLATEW+KGK++ EA +IKNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K +
Sbjct:   19 SAPTLRTQIGYHKKVVDHYENPRNVGSLDKNAKNVGTGLVGAPACGDVMKLQVEVDEAGKIVNAKFKTFGCGSAIASSSLATEWVKGKSVDEALKIKNTEIAKELSLPPVKLHCSMLAEDAIKAALADYRLKQE 152
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Zebrafish
Match: iscub (iron-sulfur cluster assembly enzyme b [Source:ZFIN;Acc:ZDB-GENE-050417-332])

HSP 1 Score: 139.813 bits (351), Expect = 2.784e-42
Identity = 65/86 (75.58%), Postives = 76/86 (88.37%), Query Frame = 1 
Query:  136 NSPKLITNF-YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIK 390
            ++P L T   YH+KV+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQ++VDE GKI++AKFKTFGCGSAIASSSLATE ++
Sbjct:   18 SAPTLRTQIGYHKKVVDHYENPRNVGSLDKNAKNVGTGLVGAPACGDVMKLQVEVDEAGKIVNAKFKTFGCGSAIASSSLATESMR 103
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Zebrafish
Match: iscub (iron-sulfur cluster assembly enzyme b [Source:ZFIN;Acc:ZDB-GENE-050417-332])

HSP 1 Score: 136.732 bits (343), Expect = 7.479e-41
Identity = 65/83 (78.31%), Postives = 76/83 (91.57%), Query Frame = 1 
Query:  286 QIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            +++VDE GKI++AKFKTFGCGSAIASSSLATEW+KGK++ EA +IKNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K +
Sbjct:   32 KVEVDEAGKIVNAKFKTFGCGSAIASSSLATEWVKGKSVDEALKIKNTEIAKELSLPPVKLHCSMLAEDAIKAALADYRLKQE 114
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Xenopus
Match: iscu (iron-sulfur cluster assembly enzyme [Source:NCBI gene;Acc:394688])

HSP 1 Score: 215.698 bits (548), Expect = 2.581e-71
Identity = 103/124 (83.06%), Postives = 112/124 (90.32%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            YH+KV+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQI+VD NGKII+AKFKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLAEDAI+AALAD+  K D
Sbjct:   27 YHKKVVDHYENPRNVGSLDKNAKNVGTGLVGAPACGDVMKLQIEVDNNGKIIEAKFKTFGCGSAIASSSLATEWVKGKTVDEAMTIKNTDIAKELCLPPVKLHCSMLAEDAIRAALADYRLKQD 150
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Mouse
Match: Iscu (iron-sulfur cluster assembly enzyme [Source:MGI Symbol;Acc:MGI:1913633])

HSP 1 Score: 219.935 bits (559), Expect = 5.805e-73
Identity = 107/153 (69.93%), Postives = 119/153 (77.78%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K +  K
Sbjct:   10 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKK 162
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Mouse
Match: Iscu (iron-sulfur cluster assembly enzyme [Source:MGI Symbol;Acc:MGI:1913633])

HSP 1 Score: 189.889 bits (481), Expect = 1.394e-60
Identity = 92/131 (70.23%), Postives = 102/131 (77.86%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCS 477
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCS
Sbjct:   10 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCS 140
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Mouse
Match: Iscu (iron-sulfur cluster assembly enzyme [Source:MGI Symbol;Acc:MGI:1913633])

HSP 1 Score: 189.889 bits (481), Expect = 1.394e-60
Identity = 92/131 (70.23%), Postives = 102/131 (77.86%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCS 477
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCS
Sbjct:   10 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCS 140
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Mouse
Match: Iscu (iron-sulfur cluster assembly enzyme [Source:MGI Symbol;Acc:MGI:1913633])

HSP 1 Score: 184.496 bits (467), Expect = 1.898e-59
Identity = 89/113 (78.76%), Postives = 98/113 (86.73%), Query Frame = 1 
Query:  148 LITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLA 486
            L + F+   V+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLA
Sbjct:   27 LFSGFF---VVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLA 136
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. UniProt/SwissProt
Match: sp|Q9D7P6|ISCU_MOUSE (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial OS=Mus musculus OX=10090 GN=Iscu PE=1 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 4.061e-72
Identity = 107/153 (69.93%), Postives = 119/153 (77.78%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K +  K
Sbjct:   10 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQESKK 162
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. UniProt/SwissProt
Match: sp|Q9H1K1|ISCU_HUMAN (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial OS=Homo sapiens OX=9606 GN=ISCU PE=1 SV=2)

HSP 1 Score: 219.164 bits (557), Expect = 9.014e-72
Identity = 107/153 (69.93%), Postives = 119/153 (77.78%), Query Frame = 1 
Query:  106 LRSKTLRFVSNSPKLITN-------FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            LR      +  SP+L           YH+KV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VDE GKI+DA+FKTFGCGSAIASSSLATEW+KGKT+ EA  IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K +  K
Sbjct:    9 LRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKK 161
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. UniProt/SwissProt
Match: sp|Q6CFQ0|ISU1_YARLI (Iron sulfur cluster assembly protein 1, mitochondrial OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ISU1 PE=3 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 1.586e-67
Identity = 95/122 (77.87%), Postives = 110/122 (90.16%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKK 528
            YHEKVLDH+ NPRNVGS +KN++ VGTGLVGAPACGDVMKLQI+VD+NG I D KFKTFGCGSAIASSS  TE ++GK++ EAG+IKNT IAKELSLPPVKLHCSMLAEDAIK+A++D+N K
Sbjct:   37 YHEKVLDHYNNPRNVGSMNKNDEDVGTGLVGAPACGDVMKLQIRVDDNGVIQDVKFKTFGCGSAIASSSYVTELVRGKSLAEAGKIKNTVIAKELSLPPVKLHCSMLAEDAIKSAISDYNSK 158
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. UniProt/SwissProt
Match: sp|O81433|ISU3_ARATH (Iron-sulfur cluster assembly protein 3 OS=Arabidopsis thaliana OX=3702 GN=ISU3 PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 2.382e-66
Identity = 95/150 (63.33%), Postives = 119/150 (79.33%), Query Frame = 1 
Query:   94 IMQSLRSKTLRFVSNSP---KLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            + Q+ +   L   S +P    +++  YH  V+DH++NPRNVGSFDKN+  VGTGLVGAP CGDVMKLQ+K D +G+IIDAKFKTFGCGSAIA+SS+ATEW+KGK++ E   IKN+ IAK LSLPPVKLHCSMLAEDAIKAA+ ++ +K D
Sbjct:    2 LRQTTKRAFLGLASQNPTPFPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGTGLVGAPQCGDVMKLQVKFDGSGQIIDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKNSQIAKHLSLPPVKLHCSMLAEDAIKAAIKNYKEKQD 151
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. UniProt/SwissProt
Match: sp|O49627|ISU1_ARATH (Iron-sulfur cluster assembly protein 1 OS=Arabidopsis thaliana OX=3702 GN=ISU1 PE=1 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.499e-66
Identity = 96/127 (75.59%), Postives = 110/127 (86.61%), Query Frame = 1 
Query:  151 ITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDE-NGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKK 528
            I   YHE V+DH++NPRNVGSFDKN+  VGTGLVGAPACGDVMKLQIKVDE  G+I+DA+FKTFGCGSAIASSS+ATEW+KGK + +   IKNT IAK LSLPPVKLHCSMLAEDAIKAA+ D+ +K
Sbjct:   25 ILRTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYKEK 151
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. TrEMBL
Match: A0A1B6K0W8 (NifU_N domain-containing protein OS=Homalodisca liturata OX=320908 GN=g.13744 PE=3 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 3.691e-77
Identity = 116/150 (77.33%), Postives = 129/150 (86.00%), Query Frame = 1 
Query:   94 IMQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            ++QS RS ++  +         FYHE V+DH+ENPRNVGS DKN+KRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEW+KGKT+ EAG++KNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K DD K
Sbjct:   15 LLQSNRSASVPVL---------FYHENVIDHYENPRNVGSMDKNDKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWVKGKTLDEAGKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALADYKVKQDDKK 155
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. TrEMBL
Match: A0A1B6G049 (NifU_N domain-containing protein OS=Cuerna arida OX=1464854 GN=g.31245 PE=3 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 1.203e-76
Identity = 117/150 (78.00%), Postives = 130/150 (86.67%), Query Frame = 1 
Query:   94 IMQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            ++QS RS ++      P L   FYHE V+DH+ENPRNVGS DKN+K+VGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEW+KGKT+ EAG++KNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K DD K
Sbjct:   15 LLQSNRSASV------PAL---FYHENVIDHYENPRNVGSMDKNDKQVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWVKGKTLDEAGKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALADYKVKQDDKK 155
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. TrEMBL
Match: A0A1L8E126 (Putative iron binding protein involved in fe-s cluster formation OS=Nyssomyia neivai OX=330878 PE=3 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.805e-74
Identity = 112/145 (77.24%), Postives = 123/145 (84.83%), Query Frame = 1 
Query:   97 MQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKN 531
            M   R+  L  V+ +  + T  YH  VLDH+ENPRNVGS DK+EK VGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEW+KGK+I EAG++KNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K 
Sbjct:    1 MSLSRASRLLQVTKTRSVPTALYHANVLDHYENPRNVGSMDKSEKNVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWVKGKSIDEAGKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALADYKIKQ 145
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. TrEMBL
Match: A0A182TKP9 (NifU_N domain-containing protein OS=Anopheles melas OX=34690 PE=3 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 1.355e-73
Identity = 109/138 (78.99%), Postives = 121/138 (87.68%), Query Frame = 1 
Query:  118 TLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKN 531
            TL  VS +  +    YH+ VL+H+ENPRNVGS DK +K VGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEW+KGKT+ +AG++KNT IAKELSLPPVKLHCSMLAEDAIKAAL D+NKK 
Sbjct:    9 TLLKVSRARSVPAALYHQNVLEHYENPRNVGSLDKKDKNVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWVKGKTLDQAGQLKNTDIAKELSLPPVKLHCSMLAEDAIKAALEDYNKKQ 146
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. TrEMBL
Match: A0A182KQ36 (NifU_N domain-containing protein OS=Anopheles coluzzii OX=1518534 PE=3 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 1.355e-73
Identity = 109/138 (78.99%), Postives = 121/138 (87.68%), Query Frame = 1 
Query:  118 TLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKN 531
            TL  VS +  +    YH+ VL+H+ENPRNVGS DK +K VGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEW+KGKT+ +AG++KNT IAKELSLPPVKLHCSMLAEDAIKAAL D+NKK 
Sbjct:    9 TLLKVSRARSVPAALYHQNVLEHYENPRNVGSLDKKDKNVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWVKGKTLDQAGQLKNTDIAKELSLPPVKLHCSMLAEDAIKAALEDYNKKQ 146
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Cavefish
Match: si:ch211-191d15.2 (iron-sulfur cluster assembly enzyme [Source:NCBI gene;Acc:103046629])

HSP 1 Score: 219.164 bits (557), Expect = 8.773e-73
Identity = 102/125 (81.60%), Postives = 114/125 (91.20%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDD 537
            YH+KV+DH+ENPRNVGS DKN + VGTGLVGAPACGDVMKLQI+VDENGKI+DA+FKTFGCGSAIASSSLATEW+KGK++ EA +IKNT IAKEL LPPVKLHCSMLAEDAIKAALAD+  K  D
Sbjct:   31 YHKKVVDHYENPRNVGSLDKNARNVGTGLVGAPACGDVMKLQIQVDENGKIVDARFKTFGCGSAIASSSLATEWVKGKSVDEALKIKNTEIAKELCLPPVKLHCSMLAEDAIKAALADYRLKQKD 155
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Yeast
Match: ISU1 (Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant [Source:SGD;Acc:S000006056])

HSP 1 Score: 184.882 bits (468), Expect = 3.574e-60
Identity = 88/126 (69.84%), Postives = 101/126 (80.16%), Query Frame = 1 
Query:  160 FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKV-DENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
             YH KV++H+ +PRNVGS DK    VGTGLVGAPACGDVM+LQIKV D  G I D KFKTFGCGSAIASSS  TE ++G T+ +A +IKNT IAKELSLPPVKLHCSMLAEDAIKAA+ D+  K +
Sbjct:   34 LYHPKVIEHYTHPRNVGSLDKKLPNVGTGLVGAPACGDVMRLQIKVNDSTGVIEDVKFKTFGCGSAIASSSYMTELVQGMTLDDAAKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKSKRN 159
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Yeast
Match: ISU2 (Mitochondrial protein required for iron-sulfur protein synthesis; performs scaffolding function during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; protein abundance increases under DNA replication stress; ISU2 has a paralog, ISU1, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant [Source:SGD;Acc:S000005752])

HSP 1 Score: 181.8 bits (460), Expect = 3.708e-59
Identity = 88/128 (68.75%), Postives = 105/128 (82.03%), Query Frame = 1 
Query:  160 FYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKV-DENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDS 540
             YH KV+DH+ NPRNVGS DK+   VGTG+VGAPACGDV+KLQI+V D++G I + KFKTFGCGSAIASSS  TE ++G ++ EA +IKNT IAKELSLPPVKLHCSMLAEDAIKAA+ D+  K + S
Sbjct:   26 LYHPKVIDHYTNPRNVGSMDKSLANVGTGIVGAPACGDVIKLQIQVNDKSGIIENVKFKTFGCGSAIASSSYMTELVRGMSLDEAVKIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYKTKRNPS 153
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Yeast
Match: YPL135C-A_mRNA (Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching [Source:SGD;Acc:S000028720])

HSP 1 Score: 74.7146 bits (182), Expect = 1.323e-18
Identity = 39/55 (70.91%), Postives = 43/55 (78.18%), Query Frame = -1 
Query:  195 IAEPHPKVLNLASIILPFSS-TLICSFITSPHAGAPTSPVPTRFSFLSKDPTFRG 356
            +AEPHPKVLNL S I P  S TLIC+ ITSPHAGAPT PVPT  +FLS +PTF G
Sbjct:    1 MAEPHPKVLNLTSSITPVESLTLICNLITSPHAGAPTRPVPTLGNFLSNEPTFLG 55
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Nematostella
Match: EDO38719 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBZ9])

HSP 1 Score: 207.608 bits (527), Expect = 1.065e-68
Identity = 98/127 (77.17%), Postives = 111/127 (87.40%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            YH+ V++H+ENP+NVGS DK++  VGTGLVGAPACGDVMKLQIKVDE GKIIDAKFKTFGCGSAIASSSLATEW+KGK++ EA  IKNT IAKEL LPPVKLHCSMLAEDAI+AAL D+  K   +K
Sbjct:   31 YHKNVIEHYENPKNVGSLDKDDATVGTGLVGAPACGDVMKLQIKVDEQGKIIDAKFKTFGCGSAIASSSLATEWVKGKSVDEAQLIKNTDIAKELCLPPVKLHCSMLAEDAIRAALNDYKVKKGKAK 157
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Medaka
Match: iscub (iron-sulfur cluster assembly scaffold protein IscU [Source:NCBI gene;Acc:101167135])

HSP 1 Score: 219.55 bits (558), Expect = 6.493e-73
Identity = 105/135 (77.78%), Postives = 117/135 (86.67%), Query Frame = 1 
Query:  142 PKLITNF-YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            P+ I+   YHEKV+DH+ENPRNVGS DK  K VGTGLVGAPACGDVMKLQI+VD  GKI+DAKFKTFGCGSAIASSSLATEW+KGK++ EA +IKNT IAKELSLPPVKLHCSMLAEDAIKAALAD+  K  D +
Sbjct:   23 PEYISQCCYHEKVVDHYENPRNVGSLDKKSKNVGTGLVGAPACGDVMKLQIEVDSQGKIVDAKFKTFGCGSAIASSSLATEWVKGKSVDEALKIKNTDIAKELSLPPVKLHCSMLAEDAIKAALADYRLKQSDGQ 157
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Medaka
Match: si:ch211-191d15.2 (iron-sulfur cluster assembly enzyme [Source:NCBI gene;Acc:101165644])

HSP 1 Score: 219.164 bits (557), Expect = 3.173e-72
Identity = 102/124 (82.26%), Postives = 114/124 (91.94%), Query Frame = 1 
Query:  163 YHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            YH+KV+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQI+VDENGKI+DAKFKTFGCGSAIASSSLATEW+KGK++ EA +IKN+ IAKEL LPPVKLHCSMLAEDAIKAAL+D+  K D
Sbjct:   68 YHKKVVDHYENPRNVGSLDKNSKSVGTGLVGAPACGDVMKLQIQVDENGKIVDAKFKTFGCGSAIASSSLATEWVKGKSLDEAMKIKNSDIAKELCLPPVKLHCSMLAEDAIKAALSDYKIKQD 191
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Medaka
Match: si:ch211-191d15.2 (iron-sulfur cluster assembly enzyme [Source:NCBI gene;Acc:101165644])

HSP 1 Score: 210.69 bits (535), Expect = 1.995e-69
Identity = 99/121 (81.82%), Postives = 111/121 (91.74%), Query Frame = 1 
Query:  172 KVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKND 534
            +V+DH+ENPRNVGS DKN K VGTGLVGAPACGDVMKLQI+VDENGKI+DAKFKTFGCGSAIASSSLATEW+KGK++ EA +IKN+ IAKEL LPPVKLHCSMLAEDAIKAAL+D+  K D
Sbjct:   38 QVVDHYENPRNVGSLDKNSKSVGTGLVGAPACGDVMKLQIQVDENGKIVDAKFKTFGCGSAIASSSLATEWVKGKSLDEAMKIKNSDIAKELCLPPVKLHCSMLAEDAIKAALSDYKIKQD 158
[Close]
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Planmine SMEST
Match: SMESG000041819.1 (SMESG000041819.1)

HSP 1 Score: 306.22 bits (783), Expect = 1.662e-107
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 1 
Query:   97 MQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 543
            MQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK
Sbjct:    1 MQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTGLVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGKTITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK 149
The following BLAST results are available for this feature:
BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ISCU1.877e-7269.93iron-sulfur cluster assembly enzyme [Source:HGNC S... [more]
ISCU1.365e-6879.84iron-sulfur cluster assembly enzyme [Source:HGNC S... [more]
ISCU2.860e-6170.23iron-sulfur cluster assembly enzyme [Source:HGNC S... [more]
ISCU3.299e-6170.23iron-sulfur cluster assembly enzyme [Source:HGNC S... [more]
ISCU3.299e-6170.23iron-sulfur cluster assembly enzyme [Source:HGNC S... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Y45F10D.41.151e-7080.49pep chromosome:WBcel235:IV:13783962:13784680:1 gen... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
IscU9.743e-7476.06gene:FBgn0037637 transcript:FBtr0081930[more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ISCU4.647e-7483.46si:ch211-191d15.2 [Source:ZFIN;Acc:ZDB-GENE-040724... [more]
iscub3.215e-7372.11iron-sulfur cluster assembly enzyme b [Source:ZFIN... [more]
iscub1.518e-7277.61iron-sulfur cluster assembly enzyme b [Source:ZFIN... [more]
iscub2.784e-4275.58iron-sulfur cluster assembly enzyme b [Source:ZFIN... [more]
iscub7.479e-4178.31iron-sulfur cluster assembly enzyme b [Source:ZFIN... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
iscu2.581e-7183.06iron-sulfur cluster assembly enzyme [Source:NCBI g... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Iscu5.805e-7369.93iron-sulfur cluster assembly enzyme [Source:MGI Sy... [more]
Iscu1.394e-6070.23iron-sulfur cluster assembly enzyme [Source:MGI Sy... [more]
Iscu1.394e-6070.23iron-sulfur cluster assembly enzyme [Source:MGI Sy... [more]
Iscu1.898e-5978.76iron-sulfur cluster assembly enzyme [Source:MGI Sy... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9D7P6|ISCU_MOUSE4.061e-7269.93Iron-sulfur cluster assembly enzyme ISCU, mitochon... [more]
sp|Q9H1K1|ISCU_HUMAN9.014e-7269.93Iron-sulfur cluster assembly enzyme ISCU, mitochon... [more]
sp|Q6CFQ0|ISU1_YARLI1.586e-6777.87Iron sulfur cluster assembly protein 1, mitochondr... [more]
sp|O81433|ISU3_ARATH2.382e-6663.33Iron-sulfur cluster assembly protein 3 OS=Arabidop... [more]
sp|O49627|ISU1_ARATH7.499e-6675.59Iron-sulfur cluster assembly protein 1 OS=Arabidop... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B6K0W83.691e-7777.33NifU_N domain-containing protein OS=Homalodisca li... [more]
A0A1B6G0491.203e-7678.00NifU_N domain-containing protein OS=Cuerna arida O... [more]
A0A1L8E1262.805e-7477.24Putative iron binding protein involved in fe-s clu... [more]
A0A182TKP91.355e-7378.99NifU_N domain-containing protein OS=Anopheles mela... [more]
A0A182KQ361.355e-7378.99NifU_N domain-containing protein OS=Anopheles colu... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:ch211-191d15.28.773e-7381.60iron-sulfur cluster assembly enzyme [Source:NCBI g... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
ISU13.574e-6069.84Conserved protein of the mitochondrial matrix; per... [more]
ISU23.708e-5968.75Mitochondrial protein required for iron-sulfur pro... [more]
YPL135C-A_mRNA1.323e-1870.91Protein of unknown function; identified by gene-tr... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO387191.065e-6877.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
iscub6.493e-7377.78iron-sulfur cluster assembly scaffold protein IscU... [more]
si:ch211-191d15.23.173e-7282.26iron-sulfur cluster assembly enzyme [Source:NCBI g... [more]
si:ch211-191d15.21.995e-6981.82iron-sulfur cluster assembly enzyme [Source:NCBI g... [more]
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BLAST of Iron-sulfur cluster assembly scaffold protein IscU vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000041819.11.662e-107100.00SMESG000041819.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030485 ID=SMED30030485|Name=Iron-sulfur cluster assembly scaffold protein IscU|organism=Schmidtea mediterranea sexual|type=transcript|length=677bp
TTTGTTTAAAAAAGGTGTTTTCAAAATTTTCAAGTTAATACCAGGGCATC
TAAATTATATATTTTTAAGCTTTTATATAAAATATTTTCTTGAATCATGC
AAAGTTTGAGATCTAAAACTCTTCGTTTCGTTTCGAACTCACCAAAATTA
ATTACTAACTTTTACCATGAAAAAGTATTGGATCATTTTGAAAATCCTCG
AAATGTTGGATCTTTCGACAAAAATGAAAAGCGTGTTGGTACTGGGCTTG
TTGGAGCACCAGCATGTGGAGATGTTATGAAACTGCAAATTAAAGTTGAT
GAAAATGGCAAAATTATTGATGCTAAATTTAAAACTTTTGGATGTGGTTC
TGCAATAGCGTCTAGTAGTTTGGCAACCGAATGGATTAAAGGTAAAACTA
TAACTGAAGCTGGCGAGATAAAAAATACGGCAATTGCGAAAGAGTTATCC
TTACCTCCAGTTAAGTTGCACTGTTCAATGCTTGCTGAAGATGCCATCAA
AGCAGCATTGGCTGATTTTAACAAGAAAAATGACGATTCAAAATAAATTT
CATTCTCATTTCCTTCATCCCATAAATTAATTTGATTTATGTTGTATTTA
GAGTGATCATATATATTATTATTCATATTTTCATCATAAATATATGTTTA
TAACTTGTTAACTATGGGATAGTTTTG
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protein sequence of SMED30030485-orf-1

>SMED30030485-orf-1 ID=SMED30030485-orf-1|Name=SMED30030485-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MQSLRSKTLRFVSNSPKLITNFYHEKVLDHFENPRNVGSFDKNEKRVGTG
LVGAPACGDVMKLQIKVDENGKIIDAKFKTFGCGSAIASSSLATEWIKGK
TITEAGEIKNTAIAKELSLPPVKLHCSMLAEDAIKAALADFNKKNDDSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR011339ISC_FeS_clus_asmbl_IscU
IPR002871NIF_FeS_clus_asmbl_NifU_N
IPR004170WWE-dom
Vocabulary: molecular function
TermDefinition
GO:0005506iron ion binding
GO:0051536iron-sulfur cluster binding
GO:0005198structural molecule activity
GO:0008198ferrous iron binding
GO:0036455iron-sulfur transferase activity
GO:0046872metal ion binding
GO:00515372 iron, 2 sulfur cluster binding
GO:00515394 iron, 4 sulfur cluster binding
Vocabulary: biological process
TermDefinition
GO:0016226iron-sulfur cluster assembly
GO:0006879cellular iron ion homeostasis
GO:0097428protein maturation by iron-sulfur cluster transfer
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0005759mitochondrial matrix
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.90.1010.10coord: 22..149
e-value: 5.4E-57
score: 193.5
NoneNo IPR availablePANTHERPTHR10093:SF16IRON-SULFUR CLUSTER ASSEMBLY ENZYME ISCU, MITOCHONDRIALcoord: 20..147
NoneNo IPR availableSUPERFAMILYSSF82649SufE/NifUcoord: 22..145
IPR011339ISC system FeS cluster assembly, IscU scaffoldTIGRFAMTIGR01999TIGR01999coord: 23..145
e-value: 3.4E-67
score: 222.2
IPR002871NIF system FeS cluster assembly, NifU, N-terminalPFAMPF01592NifU_Ncoord: 23..145
e-value: 9.2E-56
score: 187.5
IPR002871NIF system FeS cluster assembly, NifU, N-terminalPANTHERPTHR10093IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOGcoord: 20..147
IPR002871NIF system FeS cluster assembly, NifU, N-terminalCDDcd06664IscU_likecoord: 23..137
e-value: 1.52641E-50
score: 155.485