Pyruvate kinase

Overview
NamePyruvate kinase
Smed IDSMED30030481
Length (bp)1830
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pyruvate kinase (SMED30030481) t-SNE clustered cells

Violin plots show distribution of expression levels for Pyruvate kinase (SMED30030481) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pyruvate kinase (SMED30030481) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pyruvate kinase (SMED30030481) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030481

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30030481SMESG000017675.1 dd_Smed_v4_2738_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30030481SMESG000017675.1 dd_Smed_v4_2738_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030481SMESG000017675.1 dd_Smed_v4_2738_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030481SMESG000017675.1 dd_Smed_v4_2738_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pyruvate kinase vs. Ensembl Human
Match: PKM (pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021])

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 323/526 (61.41%), Postives = 405/526 (77.00%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  +Y  + FE ++      +D T +TAV AVEA+ +C + AIIV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Human
Match: PKM (pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 322/526 (61.22%), Postives = 404/526 (76.81%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++  K FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Human
Match: PKM (pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 322/526 (61.22%), Postives = 404/526 (76.81%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++  K FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Human
Match: PKM (pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 322/526 (61.22%), Postives = 404/526 (76.81%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++  K FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Human
Match: PKM (pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 322/526 (61.22%), Postives = 404/526 (76.81%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++  K FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Celegans
Match: pyk-1 (Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL40])

HSP 1 Score: 592.423 bits (1526), Expect = 0.000e+0
Identity = 298/519 (57.42%), Postives = 389/519 (74.95%), Query Frame = 2 
Query:  137 NYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            +YF Q Q++ +    +++EH  +L+  + P ++R +GIICTIGPAC SV +L K+I  GMN+ARLNFSHG++EYH  TI  VREA D+F    R I IALDTKGPEIRTGL+ G A+ E+ L +G++I++  D  +SE   +  L+VDY NI  +L V S +YIDDGLISL+V +  +D ++C VENGG+LGS K VNLPG  VDLPAVSEKD +DL+FGVE  VD++FASFIR+   I+ I+ +LGEKGK I II+KIEN EGV   DEI+  SDG+MVARGDLGIEIP EKV LAQKM+I +CNRAGKPVICATQMLESM++KP PT+AE SDVANAVLDGADCVMLSGETAKG+YP++A+++MH IC+EAE  +Y  + F+ +      PTD +H+ A+AA  AA  CHA+AI++ITTTGRSA   ++Y+P  PILT++R     RQ HL+RG+ P+ Y   R  DW  D+D RI+++++ G  RGF+   DF+++VTGW+ G G+TNTLRII  E
Sbjct:   41 DYFKQEQKLAAVPATTHMEHLCRLDIREAPHLVRQTGIICTIGPACASVEMLQKLILNGMNIARLNFSHGSHEYHAGTIANVREAADSFSDK-RVIGIALDTKGPEIRTGLLAGGASAEIELARGASIRLTTDPHFSESGTAVNLFVDYKNIAKVLSVGSRVYIDDGLISLIVEELQTDAVICSVENGGMLGSRKGVNLPGTIVDLPAVSEKDCKDLQFGVEQGVDIIFASFIRNAEGIRTIRKVLGEKGKKIKIIAKIENQEGVDNADEIISESDGVMVARGDLGIEIPAEKVFLAQKMLISKCNRAGKPVICATQMLESMVHKPRPTRAEGSDVANAVLDGADCVMLSGETAKGEYPVDALKIMHYICKEAEAAVYHRRLFDELLQNTQKPTDMSHTIAIAATSAAASCHASAILLITTTGRSAIQCSRYKPAVPILTISRDVAVCRQLHLYRGVFPVHYPAERAADWPTDVDNRINHAIAIGKDRGFIHKGDFLVVVTGWRQGAGATNTLRIITAE 558
[Close]
BLAST of Pyruvate kinase vs. Ensembl Celegans
Match: pyk-1 (Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL40])

HSP 1 Score: 591.652 bits (1524), Expect = 0.000e+0
Identity = 298/519 (57.42%), Postives = 389/519 (74.95%), Query Frame = 2 
Query:  137 NYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            +YF Q Q++ +    +++EH  +L+  + P ++R +GIICTIGPAC SV +L K+I  GMN+ARLNFSHG++EYH  TI  VREA D+F    R I IALDTKGPEIRTGL+ G A+ E+ L +G++I++  D  +SE   +  L+VDY NI  +L V S +YIDDGLISL+V +  +D ++C VENGG+LGS K VNLPG  VDLPAVSEKD +DL+FGVE  VD++FASFIR+   I+ I+ +LGEKGK I II+KIEN EGV   DEI+  SDG+MVARGDLGIEIP EKV LAQKM+I +CNRAGKPVICATQMLESM++KP PT+AE SDVANAVLDGADCVMLSGETAKG+YP++A+++MH IC+EAE  +Y  + F+ +      PTD +H+ A+AA  AA  CHA+AI++ITTTGRSA   ++Y+P  PILT++R     RQ HL+RG+ P+ Y   R  DW  D+D RI+++++ G  RGF+   DF+++VTGW+ G G+TNTLRII  E
Sbjct:   45 DYFKQEQKLAAVPATTHMEHLCRLDIREAPHLVRQTGIICTIGPACASVEMLQKLILNGMNIARLNFSHGSHEYHAGTIANVREAADSFSDK-RVIGIALDTKGPEIRTGLLAGGASAEIELARGASIRLTTDPHFSESGTAVNLFVDYKNIAKVLSVGSRVYIDDGLISLIVEELQTDAVICSVENGGMLGSRKGVNLPGTIVDLPAVSEKDCKDLQFGVEQGVDIIFASFIRNAEGIRTIRKVLGEKGKKIKIIAKIENQEGVDNADEIISESDGVMVARGDLGIEIPAEKVFLAQKMLISKCNRAGKPVICATQMLESMVHKPRPTRAEGSDVANAVLDGADCVMLSGETAKGEYPVDALKIMHYICKEAEAAVYHRRLFDELLQNTQKPTDMSHTIAIAATSAAASCHASAILLITTTGRSAIQCSRYKPAVPILTISRDVAVCRQLHLYRGVFPVHYPAERAADWPTDVDNRINHAIAIGKDRGFIHKGDFLVVVTGWRQGAGATNTLRIITAE 562
[Close]
BLAST of Pyruvate kinase vs. Ensembl Celegans
Match: pyk-1 (Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL40])

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 299/525 (56.95%), Postives = 390/525 (74.29%), Query Frame = 2 
Query:  119 KYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            K  E + Y  Q Q++ +    +++EH  +L+  + P ++R +GIICTIGPAC SV +L K+I  GMN+ARLNFSHG++EYH  TI  VREA D+F    R I IALDTKGPEIRTGL+ G A+ E+ L +G++I++  D  +SE   +  L+VDY NI  +L V S +YIDDGLISL+V +  +D ++C VENGG+LGS K VNLPG  VDLPAVSEKD +DL+FGVE  VD++FASFIR+   I+ I+ +LGEKGK I II+KIEN EGV   DEI+  SDG+MVARGDLGIEIP EKV LAQKM+I +CNRAGKPVICATQMLESM++KP PT+AE SDVANAVLDGADCVMLSGETAKG+YP++A+++MH IC+EAE  +Y  + F+ +      PTD +H+ A+AA  AA  CHA+AI++ITTTGRSA   ++Y+P  PILT++R     RQ HL+RG+ P+ Y   R  DW  D+D RI+++++ G  RGF+   DF+++VTGW+ G G+TNTLRII  E
Sbjct:    8 KELEASGYDKQEQKLAAVPATTHMEHLCRLDIREAPHLVRQTGIICTIGPACASVEMLQKLILNGMNIARLNFSHGSHEYHAGTIANVREAADSFSDK-RVIGIALDTKGPEIRTGLLAGGASAEIELARGASIRLTTDPHFSESGTAVNLFVDYKNIAKVLSVGSRVYIDDGLISLIVEELQTDAVICSVENGGMLGSRKGVNLPGTIVDLPAVSEKDCKDLQFGVEQGVDIIFASFIRNAEGIRTIRKVLGEKGKKIKIIAKIENQEGVDNADEIISESDGVMVARGDLGIEIPAEKVFLAQKMLISKCNRAGKPVICATQMLESMVHKPRPTRAEGSDVANAVLDGADCVMLSGETAKGEYPVDALKIMHYICKEAEAAVYHRRLFDELLQNTQKPTDMSHTIAIAATSAAASCHASAILLITTTGRSAIQCSRYKPAVPILTISRDVAVCRQLHLYRGVFPVHYPAERAADWPTDVDNRINHAIAIGKDRGFIHKGDFLVVVTGWRQGAGATNTLRIITAE 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Celegans
Match: pyk-1 (Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL40])

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 299/525 (56.95%), Postives = 390/525 (74.29%), Query Frame = 2 
Query:  119 KYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            K  E + Y  Q Q++ +    +++EH  +L+  + P ++R +GIICTIGPAC SV +L K+I  GMN+ARLNFSHG++EYH  TI  VREA D+F    R I IALDTKGPEIRTGL+ G A+ E+ L +G++I++  D  +SE   +  L+VDY NI  +L V S +YIDDGLISL+V +  +D ++C VENGG+LGS K VNLPG  VDLPAVSEKD +DL+FGVE  VD++FASFIR+   I+ I+ +LGEKGK I II+KIEN EGV   DEI+  SDG+MVARGDLGIEIP EKV LAQKM+I +CNRAGKPVICATQMLESM++KP PT+AE SDVANAVLDGADCVMLSGETAKG+YP++A+++MH IC+EAE  +Y  + F+ +      PTD +H+ A+AA  AA  CHA+AI++ITTTGRSA   ++Y+P  PILT++R     RQ HL+RG+ P+ Y   R  DW  D+D RI+++++ G  RGF+   DF+++VTGW+ G G+TNTLRII  E
Sbjct:    8 KELEASGYDKQEQKLAAVPATTHMEHLCRLDIREAPHLVRQTGIICTIGPACASVEMLQKLILNGMNIARLNFSHGSHEYHAGTIANVREAADSFSDK-RVIGIALDTKGPEIRTGLLAGGASAEIELARGASIRLTTDPHFSESGTAVNLFVDYKNIAKVLSVGSRVYIDDGLISLIVEELQTDAVICSVENGGMLGSRKGVNLPGTIVDLPAVSEKDCKDLQFGVEQGVDIIFASFIRNAEGIRTIRKVLGEKGKKIKIIAKIENQEGVDNADEIISESDGVMVARGDLGIEIPAEKVFLAQKMLISKCNRAGKPVICATQMLESMVHKPRPTRAEGSDVANAVLDGADCVMLSGETAKGEYPVDALKIMHYICKEAEAAVYHRRLFDELLQNTQKPTDMSHTIAIAATSAAASCHASAILLITTTGRSAIQCSRYKPAVPILTISRDVAVCRQLHLYRGVFPVHYPAERAADWPTDVDNRINHAIAIGKDRGFIHKGDFLVVVTGWRQGAGATNTLRIITAE 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Celegans
Match: pyk-1 (Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL40])

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 298/519 (57.42%), Postives = 389/519 (74.95%), Query Frame = 2 
Query:  137 NYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            +YF Q Q++ +    +++EH  +L+  + P ++R +GIICTIGPAC SV +L K+I  GMN+ARLNFSHG++EYH  TI  VREA D+F    R I IALDTKGPEIRTGL+ G A+ E+ L +G++I++  D  +SE   +  L+VDY NI  +L V S +YIDDGLISL+V +  +D ++C VENGG+LGS K VNLPG  VDLPAVSEKD +DL+FGVE  VD++FASFIR+   I+ I+ +LGEKGK I II+KIEN EGV   DEI+  SDG+MVARGDLGIEIP EKV LAQKM+I +CNRAGKPVICATQMLESM++KP PT+AE SDVANAVLDGADCVMLSGETAKG+YP++A+++MH IC+EAE  +Y  + F+ +      PTD +H+ A+AA  AA  CHA+AI++ITTTGRSA   ++Y+P  PILT++R     RQ HL+RG+ P+ Y   R  DW  D+D RI+++++ G  RGF+   DF+++VTGW+ G G+TNTLRII  E
Sbjct:  396 DYFKQEQKLAAVPATTHMEHLCRLDIREAPHLVRQTGIICTIGPACASVEMLQKLILNGMNIARLNFSHGSHEYHAGTIANVREAADSFSDK-RVIGIALDTKGPEIRTGLLAGGASAEIELARGASIRLTTDPHFSESGTAVNLFVDYKNIAKVLSVGSRVYIDDGLISLIVEELQTDAVICSVENGGMLGSRKGVNLPGTIVDLPAVSEKDCKDLQFGVEQGVDIIFASFIRNAEGIRTIRKVLGEKGKKIKIIAKIENQEGVDNADEIISESDGVMVARGDLGIEIPAEKVFLAQKMLISKCNRAGKPVICATQMLESMVHKPRPTRAEGSDVANAVLDGADCVMLSGETAKGEYPVDALKIMHYICKEAEAAVYHRRLFDELLQNTQKPTDMSHTIAIAATSAAASCHASAILLITTTGRSAIQCSRYKPAVPILTISRDVAVCRQLHLYRGVFPVHYPAERAADWPTDVDNRINHAIAIGKDRGFIHKGDFLVVVTGWRQGAGATNTLRIITAE 913
[Close]
BLAST of Pyruvate kinase vs. Ensembl Fly
Match: PyK (gene:FBgn0267385 transcript:FBtr0084213)

HSP 1 Score: 601.282 bits (1549), Expect = 0.000e+0
Identity = 296/507 (58.38%), Postives = 377/507 (74.36%), Query Frame = 2 
Query:  182 SNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            + LEH  +L ++     +R SGI+CTIGPA  SV +L KM++ GMN+AR+NFSHG++EYH  T+  VR+AV  +     Y  P+AIALDTKGPEIRTGL+ GS T E+ L KG  IK+  + ++ E+ +   ++VDY NI+N+++  + +++DDGLISL+V + G D L CEVENGG LGS K VNLPG  VDLPAVSEKDK DL FGVE  VDM+FASFIR+  A+ EI+ +LGEKGK+I IISKIEN +G+   DEI+E  DGIMVARGDLGIEIP EKV LAQK MI RCN+AGKPVICATQMLESM+ KP PT+AEISDVANAVLDGADCVMLSGETAKG+YPLE V  M   C+EAE  L+    F  +    +G  D +H+ A+AAVEAA +  A+AI+VITT+G+SA  ++KYRP CPI+ VTR   TARQ+HL+RGL P+ Y EP + DW +D+D R+ + L  G + GF++  D V++VTGWK G G TNT+RI+ +E
Sbjct:    7 TQLEHMCRLQFDSPVPHVRLSGIVCTIGPASSSVEMLEKMMATGMNIARMNFSHGSHEYHAATVANVRQAVKNYSAKLGYEHPVAIALDTKGPEIRTGLIGGSGTAEIELKKGEKIKLTTNKEFLEKGSLEIVYVDYENIVNVVKPGNRVFVDDGLISLIVREVGKDSLTCEVENGGSLGSRKGVNLPGVPVDLPAVSEKDKSDLLFGVEQEVDMIFASFIRNAAALTEIRKVLGEKGKNIKIISKIENQQGMHNLDEIIEAGDGIMVARGDLGIEIPAEKVFLAQKAMIARCNKAGKPVICATQMLESMVKKPRPTRAEISDVANAVLDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHQNLFNDLVRG-AGTIDASHAAAIAAVEAATKAKASAIVVITTSGKSAFQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLIYKEPGLGDWLKDVDVRVQFGLQVGKKNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTVE 512
[Close]
BLAST of Pyruvate kinase vs. Ensembl Fly
Match: PyK (gene:FBgn0267385 transcript:FBtr0084214)

HSP 1 Score: 601.282 bits (1549), Expect = 0.000e+0
Identity = 296/507 (58.38%), Postives = 377/507 (74.36%), Query Frame = 2 
Query:  182 SNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            + LEH  +L ++     +R SGI+CTIGPA  SV +L KM++ GMN+AR+NFSHG++EYH  T+  VR+AV  +     Y  P+AIALDTKGPEIRTGL+ GS T E+ L KG  IK+  + ++ E+ +   ++VDY NI+N+++  + +++DDGLISL+V + G D L CEVENGG LGS K VNLPG  VDLPAVSEKDK DL FGVE  VDM+FASFIR+  A+ EI+ +LGEKGK+I IISKIEN +G+   DEI+E  DGIMVARGDLGIEIP EKV LAQK MI RCN+AGKPVICATQMLESM+ KP PT+AEISDVANAVLDGADCVMLSGETAKG+YPLE V  M   C+EAE  L+    F  +    +G  D +H+ A+AAVEAA +  A+AI+VITT+G+SA  ++KYRP CPI+ VTR   TARQ+HL+RGL P+ Y EP + DW +D+D R+ + L  G + GF++  D V++VTGWK G G TNT+RI+ +E
Sbjct:   28 TQLEHMCRLQFDSPVPHVRLSGIVCTIGPASSSVEMLEKMMATGMNIARMNFSHGSHEYHAATVANVRQAVKNYSAKLGYEHPVAIALDTKGPEIRTGLIGGSGTAEIELKKGEKIKLTTNKEFLEKGSLEIVYVDYENIVNVVKPGNRVFVDDGLISLIVREVGKDSLTCEVENGGSLGSRKGVNLPGVPVDLPAVSEKDKSDLLFGVEQEVDMIFASFIRNAAALTEIRKVLGEKGKNIKIISKIENQQGMHNLDEIIEAGDGIMVARGDLGIEIPAEKVFLAQKAMIARCNKAGKPVICATQMLESMVKKPRPTRAEISDVANAVLDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHQNLFNDLVRG-AGTIDASHAAAIAAVEAATKAKASAIVVITTSGKSAFQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLIYKEPGLGDWLKDVDVRVQFGLQVGKKNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTVE 533
[Close]
BLAST of Pyruvate kinase vs. Ensembl Fly
Match: CG2964 (gene:FBgn0031462 transcript:FBtr0077722)

HSP 1 Score: 437.958 bits (1125), Expect = 2.353e-147
Identity = 213/504 (42.26%), Postives = 328/504 (65.08%), Query Frame = 2 
Query:  182 SNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRII 1684
            + L H  +L+   +    R   +I TI  + ++   ++ MI  G+N+ RLNFSH ++E H +TI+L+ EA++         R +AIA DT+GP+IRTGL++G    +V L  G  +++ ++    ++ N   ++VDY NIIN+ +    ++IDDG + L +++ G D L+CEV +GG L +N  V LP  ++DLPAVSEKD  D++F ++ NVD +FAS +RS   +KE++ +LGEKGKHI II+K+++   + +F EIL  +DG++++R DLG +IP EK+ + QK ++G+CN+ GKPVI A+ +LESM   P PT+AE  D+ANA++DGADC+MLS E A G +P E V    ++CREAE VL+    F  +   + G  D  HS A+AAVE A+R +A  IIV+TT+GRSA L++K+RP CPI+ +TR   TAR  +L RG+ PI Y      D+  D+D R+ ++++   + G +   D ++IV+ WK G G TN +R++
Sbjct:   14 TQLSHICELDLAQQASHQRLVSLIATISVSSRNADTIYTMIMRGVNIFRLNFSHESHEMHSKTIELINEALERIHKETGQIRTVAIAADTRGPQIRTGLLDG----DVFLRSGDNLRLSINRDLYDKGNKEAVYVDYPNIINLTKTGDRLFIDDGRLLLHILEVGVDGLLCEVIHGGQLNNNCNVILPEIEIDLPAVSEKDMFDIQFSIKANVDFLFASAVRSAKNVKELRTVLGEKGKHIKIIAKMDSKIALSRFSEILRAADGLLLSRADLGTQIPIEKLFITQKSILGQCNKVGKPVIVASHILESMRTLPHPTRAECFDLANAIIDGADCIMLSSEVAIGSFPKETVATCDTLCREAEKVLWFRDLFSDLVSEVRGELDAAHSLAIAAVETAKRTNATLIIVLTTSGRSATLVSKFRPRCPIMAITRCERTARWVYLHRGVLPILYTLEPSTDYATDVDARVQFAMTSAKKWGIIDDGDPIVIVSAWKDGGGFTNNVRVV 513
[Close]
BLAST of Pyruvate kinase vs. Ensembl Fly
Match: CG7069 (gene:FBgn0038952 transcript:FBtr0084215)

HSP 1 Score: 413.305 bits (1061), Expect = 2.129e-135
Identity = 215/468 (45.94%), Postives = 295/468 (63.03%), Query Frame = 2 
Query:  314 MNVARLNFSHGTYEYHEETIKLVREAVDTFKPYF---RPIAIALDTKGPEIRTG-LVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTI-SGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRR----GFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            M V R+NFSHG++EYH +TI+  R+A+  +       R +AIALDTKGPEIRTG L  G+   E+ L  G  + +    + +++ N   ++VDY  +  +++  + +++DDGLI+L+V +   D ++C+VENGG LGS+K +NLPG  VDLP+V+EKDK+DL+FG E  VDM+FASFIR  NA+KEI+ +LG  G  I IISKIEN +G+   D+I+  SDGIMVARGD+GIEIP E V LAQK ++ +CN+ GKPVICATQM+ESM NKP PT+AE SDVANA+ DG D VMLSGETAKG+YP+E VQ M  IC + E VL+      ++K  I +   D   +   A  EAA    A AI+V +     A +++  RP CPI+ +T +   A QS L+RG++P+   E  M+              S+ FRR    G   M    I+  G K  V   N +   +I
Sbjct:    1 MRVVRMNFSHGSHEYHCQTIQAARKAIAMYVEQTGLPRTLAIALDTKGPEIRTGKLAGGNDRAEIELKTGDKVTLSTKKEMADKSNKDNIYVDYQRLPQLVKPGNRVFVDDGLIALIVKESKGDEVICQVENGGKLGSHKGINLPGVPVDLPSVTEKDKQDLKFGAEQKVDMIFASFIRDANALKEIRQVLGPAGACIKIISKIENHQGLVNIDDIIRESDGIMVARGDMGIEIPTEDVPLAQKSIVAKCNKVGKPVICATQMMESMTNKPRPTRAEASDVANAIFDGCDAVMLSGETAKGKYPVECVQCMARICAKVEAVLWYESLQNSLKREIRTSAADHISAVTTAIAEAATVGQARAIVVASPCSMVAQMVSHMRPPCPIVMLTGNESEAAQSLLFRGIYPLLVEE--MVIG------------SFNFRRIMQSGLKLMGKMDILEPGQKGSVVLVNAMSAEKI 454
[Close]
BLAST of Pyruvate kinase vs. Ensembl Fly
Match: CG7362 (gene:FBgn0038258 transcript:FBtr0331700)

HSP 1 Score: 249.98 bits (637), Expect = 7.075e-73
Identity = 128/275 (46.55%), Postives = 179/275 (65.09%), Query Frame = 2 
Query:  725 VNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGE--KGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISG-----PTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNE 1528
            +N  G   DL A++E+DK DL+FG +  VDM+FASFIR   A+KEI+  LG     +HI IISKIE+ + +   DEI+  SDGIMVA G++G EI  E V LAQK ++ +CN+ GKPVICA QM+ SMI KP PT+AE SDVANA+LDG D ++LS ETAKG+YP++ VQ M  IC + E+VL+    +E+I++ +         D   + + A  EAA    A AI+V +     + ++++ RP CPI+ +T  P  A QS L+RG++P+   E
Sbjct:  109 INPQGVAADLNAITEQDKLDLKFGADQKVDMIFASFIRDAKALKEIRQALGACPSSEHIKIISKIESQQALANIDEIIRESDGIMVALGNMGNEIALEAVPLAQKSIVAKCNKVGKPVICANQMMNSMITKPRPTRAESSDVANAILDGCDALVLSDETAKGKYPVQCVQCMARICAKVESVLW----YESIQNNLKSEVRINAADHISAVSTAIAEAATVSQAQAIVVASPCSIVSQMVSQMRPPCPIVLLTGCPHEAAQSLLFRGVYPLLVKE 379

HSP 2 Score: 74.7146 bits (182), Expect = 1.250e-13
Identity = 42/114 (36.84%), Postives = 62/114 (54.39%), Query Frame = 2 
Query:  125 FEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYF---RPIAIALDTKGPEI 457
             EP  +     R+    Y S L++ S+L +      +  + IICTIGP+     +L  +I AGM V RL+FS GT++ H + I+  R+A+  +       R +AIALDTKGP I
Sbjct:    1 MEPKKF-----RLDRGPYLSQLDYQSRLQFQAPALRLPLTSIICTIGPSSSQPEVLLNLIHAGMKVVRLDFSDGTHDCHCQAIQAARKAIAMYAEETGLPRSLAIALDTKGPVI 109
[Close]
BLAST of Pyruvate kinase vs. Ensembl Zebrafish
Match: pkma (pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-031201-4])

HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0
Identity = 313/519 (60.31%), Postives = 399/519 (76.88%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            FIQ Q++ +    + LEH   L+ + EP + RN+GIICTIGPA +SV  L +MI +GMN+AR+NFSHG++EYH ETIK VREA  +F+P    +RP+ IALDTKGPEIRTGL+ GS T EV L KG+ IK+ +D+ + E C+   LW+DY NI  ++EV S +YIDDGLISL V++ GSD+L+CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDM+FASFIR    ++ ++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCN+AGKP+ICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE   +  + FE ++ +     D + + AV AVEA+ +C A+ II++T TGRSAHLI++YRP  PIL VTR+  TARQ+HL+RG+ PI+YN P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   13 FIQTQQLNAAMADTFLEHMCLLDIDSEPTIARNTGIICTIGPASRSVDTLKEMIKSGMNIARMNFSHGSHEYHGETIKNVREACASFEPGSIQYRPVGIALDTKGPEIRTGLIKGSGTAEVELKKGNKIKVTLDDSFMENCDEDTLWLDYKNITKVVEVGSKVYIDDGLISLQVLQIGSDYLICEIENGGSLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMIFASFIRKAADVQAVRKVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNKAGKPIICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAATFHRQLFEGLRRSSVLTRDPSDAVAVGAVEASFKCCASGIIILTKTGRSAHLISRYRPRAPILAVTRNEQTARQAHLYRGIFPIYYNSPSNDVWAEDVDLRVNFAMDVGKARGFFKAGDVVIVLTGWRPGSGYTNTMRVVPV 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Zebrafish
Match: pkma (pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-031201-4])

HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0
Identity = 313/519 (60.31%), Postives = 399/519 (76.88%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            FIQ Q++ +    + LEH   L+ + EP + RN+GIICTIGPA +SV  L +MI +GMN+AR+NFSHG++EYH ETIK VREA  +F+P    +RP+ IALDTKGPEIRTGL+ GS T EV L KG+ IK+ +D+ + E C+   LW+DY NI  ++EV S +YIDDGLISL V++ GSD+L+CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDM+FASFIR    ++ ++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCN+AGKP+ICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE   +  + FE ++ +     D + + AV AVEA+ +C A+ II++T TGRSAHLI++YRP  PIL VTR+  TARQ+HL+RG+ PI+YN P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   13 FIQTQQLNAAMADTFLEHMCLLDIDSEPTIARNTGIICTIGPASRSVDTLKEMIKSGMNIARMNFSHGSHEYHGETIKNVREACASFEPGSIQYRPVGIALDTKGPEIRTGLIKGSGTAEVELKKGNKIKVTLDDSFMENCDEDTLWLDYKNITKVVEVGSKVYIDDGLISLQVLQIGSDYLICEIENGGSLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMIFASFIRKAADVQAVRKVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNKAGKPIICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAATFHRQLFEGLRRSSVLTRDPSDAVAVGAVEASFKCCASGIIILTKTGRSAHLISRYRPRAPILAVTRNEQTARQAHLYRGIFPIYYNSPSNDVWAEDVDLRVNFAMDVGKARGFFKAGDVVIVLTGWRPGSGYTNTMRVVPV 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Zebrafish
Match: pkma (pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-031201-4])

HSP 1 Score: 664.84 bits (1714), Expect = 0.000e+0
Identity = 311/519 (59.92%), Postives = 399/519 (76.88%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            FIQ Q++ +    + LEH   L+ + EP + RN+GIICTIGPA +SV  L +MI +GMN+AR+NFSHG++EYH ETIK VREA  +F+P    +RP+ IALDTKGPEIRTGL+ GS T EV L KG+ IK+ +D+ + E C+   LW+DY NI  ++EV S +YIDDGLISL V++ GSD+L+CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDM+FASFIR    ++ ++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCN+AGKP+ICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++  +   TD   + A+ AVEA+ +  ++A IV+T +GRSAHLI++YRP  PIL VTR+  TARQ+HL+RG+ PI+YN P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   13 FIQTQQLNAAMADTFLEHMCLLDIDSEPTIARNTGIICTIGPASRSVDTLKEMIKSGMNIARMNFSHGSHEYHGETIKNVREACASFEPGSIQYRPVGIALDTKGPEIRTGLIKGSGTAEVELKKGNKIKVTLDDSFMENCDEDTLWLDYKNITKVVEVGSKVYIDDGLISLQVLQIGSDYLICEIENGGSLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMIFASFIRKAADVQAVRKVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNKAGKPIICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQVFEDLRRCLPLSTDPAEAIAIGAVEASFKILSSAFIVLTGSGRSAHLISRYRPRAPILAVTRNEQTARQAHLYRGIFPIYYNSPSNDVWAEDVDLRVNFAMDVGKARGFFKAGDVVIVLTGWRPGSGYTNTMRVVPV 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Zebrafish
Match: pkmb (pyruvate kinase M1/2b [Source:ZFIN;Acc:ZDB-GENE-040801-230])

HSP 1 Score: 650.203 bits (1676), Expect = 0.000e+0
Identity = 312/517 (60.35%), Postives = 391/517 (75.63%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPY---FRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRII 1684
            FIQ Q++ +    + LEH   L+ + EP V RN+GI+CT+GPA +S+  L +MI +GMNVARLNFSHGT+EYH ETIK VREA+++F      +RP+AIALDTKGPEIRTGL+ GS T EV L KG+ IK+ +D+K+ + C+ + LW+DY NI  +++  S IY+DDGLISL V + GSDFL CE+ENGG+LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    +  ++ +LGEKGK I IISK+EN EGV+KFDEILE SDGIMVARGDLGIEIP EKV LAQKMMI RCNR GKP+ICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG+YP+E+V   H I REAE  ++  + FE ++ T     D T S AV AVEA+ +C A+AII +T TGRSA L+++YRP  PI+ VTR+  T+RQ HL+RG+ PI Y +P    W ED+D R+ ++L  G  R + +  D +I+VTGW+ G G TNT+RI+
Sbjct:   11 FIQTQQMPAAMAETFLEHLCLLDIDSEPTVSRNTGIVCTLGPASRSLETLREMILSGMNVARLNFSHGTHEYHAETIKSVREAIESFGAGTIDYRPVAIALDTKGPEIRTGLIKGSGTEEVKLVKGNIIKLTLDDKFMDNCDENTLWLDYKNITKVVQQGSHIYVDDGLISLKVKEIGSDFLNCEIENGGMLGSKKGVNLPGANVDLPAVSEKDIKDLQFGVEQGVDMVFASFIRKAADVHAVRKVLGEKGKDIRIISKLENHEGVRKFDEILEASDGIMVARGDLGIEIPTEKVFLAQKMMISRCNRIGKPIICATQMLESMIKKPRPTRAESSDVANAVLDGADCIMLSGETAKGEYPIESVLTQHLIAREAEAAMFHRQLFEELRRTSHLTRDPTESVAVGAVEASFKCCASAIICLTKTGRSAQLLSRYRPRAPIMAVTRNGQTSRQLHLYRGVIPILYTKPANDIWAEDVDLRVSFALEIGKHRKYFKSGDVIIVVTGWRPGSGYTNTMRIV 527
[Close]
BLAST of Pyruvate kinase vs. Ensembl Zebrafish
Match: pkmb (pyruvate kinase M1/2b [Source:ZFIN;Acc:ZDB-GENE-040801-230])

HSP 1 Score: 650.203 bits (1676), Expect = 0.000e+0
Identity = 312/517 (60.35%), Postives = 391/517 (75.63%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPY---FRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRII 1684
            FIQ Q++ +    + LEH   L+ + EP V RN+GI+CT+GPA +S+  L +MI +GMNVARLNFSHGT+EYH ETIK VREA+++F      +RP+AIALDTKGPEIRTGL+ GS T EV L KG+ IK+ +D+K+ + C+ + LW+DY NI  +++  S IY+DDGLISL V + GSDFL CE+ENGG+LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    +  ++ +LGEKGK I IISK+EN EGV+KFDEILE SDGIMVARGDLGIEIP EKV LAQKMMI RCNR GKP+ICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG+YP+E+V   H I REAE  ++  + FE ++ T     D T S AV AVEA+ +C A+AII +T TGRSA L+++YRP  PI+ VTR+  T+RQ HL+RG+ PI Y +P    W ED+D R+ ++L  G  R + +  D +I+VTGW+ G G TNT+RI+
Sbjct:   11 FIQTQQMPAAMAETFLEHLCLLDIDSEPTVSRNTGIVCTLGPASRSLETLREMILSGMNVARLNFSHGTHEYHAETIKSVREAIESFGAGTIDYRPVAIALDTKGPEIRTGLIKGSGTEEVKLVKGNIIKLTLDDKFMDNCDENTLWLDYKNITKVVQQGSHIYVDDGLISLKVKEIGSDFLNCEIENGGMLGSKKGVNLPGANVDLPAVSEKDIKDLQFGVEQGVDMVFASFIRKAADVHAVRKVLGEKGKDIRIISKLENHEGVRKFDEILEASDGIMVARGDLGIEIPTEKVFLAQKMMISRCNRIGKPIICATQMLESMIKKPRPTRAESSDVANAVLDGADCIMLSGETAKGEYPIESVLTQHLIAREAEAAMFHRQLFEELRRTSHLTRDPTESVAVGAVEASFKCCASAIICLTKTGRSAQLLSRYRPRAPIMAVTRNGQTSRQLHLYRGVIPILYTKPANDIWAEDVDLRVSFALEIGKHRKYFKSGDVIIVVTGWRPGSGYTNTMRIV 527
[Close]
BLAST of Pyruvate kinase vs. Ensembl Xenopus
Match: pkm (pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224])

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 314/522 (60.15%), Postives = 404/522 (77.39%), Query Frame = 2 
Query:  134 TNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            ++ FIQ Q++ +    + LEH  +L+ + EP V RN+GIICTIGPA +SV IL +MI +GMN+ARLNFSHGT+EYH  TIK VREA ++F     ++RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T++I +D+ + E+C+ + LWVDY N+  +++  S IY+DDGLISL+V + G+DF + EVENGG+LGS K VNLPGA VDLPAVS KD +DL+FGVE NVDMVFASFIR    +  ++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I REAE  ++  + FE ++       D T +TAV AVE++ +C + AIIV+T +GRSAHL+++YRP  PI+ VTR+  TARQ+HL+RG+ P+ Y E     W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    6 SSAFIQTQQLHAAMADTFLEHMCRLDIDSEPIVARNTGIICTIGPASRSVEILKEMIKSGMNIARLNFSHGTHEYHAGTIKNVREATESFSSNPIHYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLRITLDDAFQEKCDENILWVDYKNLTKVVKPGSKIYVDDGLISLLVKETGADFCLTEVENGGMLGSKKGVNLPGAAVDLPAVSTKDIQDLQFGVEQNVDMVFASFIRKAADVHAVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIAREAEAAIFHRQLFEELRRVSPLTRDPTEATAVGAVESSFKCSSGAIIVLTKSGRSAHLVSRYRPRAPIIAVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDMRVNFAMDIGKARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 527
[Close]
BLAST of Pyruvate kinase vs. Ensembl Xenopus
Match: pkm (pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224])

HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0
Identity = 315/524 (60.11%), Postives = 404/524 (77.10%), Query Frame = 2 
Query:  128 EPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            E  + FIQ Q++ +    + LEH  +L+ + EP V RN+GIICTIGPA +SV IL +MI +GMN+ARLNFSHGT+EYH  TIK VREA ++F     ++RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T++I +D+ + E+C+ + LWVDY N+  +++  S IY+DDGLISL+V + G+DF + EVENGG+LGS K VNLPGA VDLPAVS KD +DL+FGVE NVDMVFASFIR    +  ++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I REAE  ++  + FE ++       D T +TAV AVE++ +C + AIIV+T +GRSAHL+++YRP  PI+ VTR+  TARQ+HL+RG+ P+ Y E     W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    4 EAGSAFIQTQQLHAAMADTFLEHMCRLDIDSEPIVARNTGIICTIGPASRSVEILKEMIKSGMNIARLNFSHGTHEYHAGTIKNVREATESFSSNPIHYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLRITLDDAFQEKCDENILWVDYKNLTKVVKPGSKIYVDDGLISLLVKETGADFCLTEVENGGMLGSKKGVNLPGAAVDLPAVSTKDIQDLQFGVEQNVDMVFASFIRKAADVHAVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIAREAEAAIFHRQLFEELRRVSPLTRDPTEATAVGAVESSFKCSSGAIIVLTKSGRSAHLVSRYRPRAPIIAVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDMRVNFAMDIGKARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 527
[Close]
BLAST of Pyruvate kinase vs. Ensembl Xenopus
Match: pkm (pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224])

HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0
Identity = 315/524 (60.11%), Postives = 404/524 (77.10%), Query Frame = 2 
Query:  128 EPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            E  + FIQ Q++ +    + LEH  +L+ + EP V RN+GIICTIGPA +SV IL +MI +GMN+ARLNFSHGT+EYH  TIK VREA ++F     ++RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T++I +D+ + E+C+ + LWVDY N+  +++  S IY+DDGLISL+V + G+DF + EVENGG+LGS K VNLPGA VDLPAVS KD +DL+FGVE NVDMVFASFIR    +  ++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I REAE  ++  + FE ++       D T +TAV AVE++ +C + AIIV+T +GRSAHL+++YRP  PI+ VTR+  TARQ+HL+RG+ P+ Y E     W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    6 EAGSAFIQTQQLHAAMADTFLEHMCRLDIDSEPIVARNTGIICTIGPASRSVEILKEMIKSGMNIARLNFSHGTHEYHAGTIKNVREATESFSSNPIHYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLRITLDDAFQEKCDENILWVDYKNLTKVVKPGSKIYVDDGLISLLVKETGADFCLTEVENGGMLGSKKGVNLPGAAVDLPAVSTKDIQDLQFGVEQNVDMVFASFIRKAADVHAVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIAREAEAAIFHRQLFEELRRVSPLTRDPTEATAVGAVESSFKCSSGAIIVLTKSGRSAHLVSRYRPRAPIIAVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDMRVNFAMDIGKARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 529
[Close]
BLAST of Pyruvate kinase vs. Ensembl Xenopus
Match: pkm (pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224])

HSP 1 Score: 652.899 bits (1683), Expect = 0.000e+0
Identity = 314/531 (59.13%), Postives = 403/531 (75.89%), Query Frame = 2 
Query:  107 LNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            L G    E  + FIQ Q++ +    + LEH  +L+ + EP V RN+GIICTIGPA +SV IL +MI +GMN+ARLNFSHGT+EYH  TIK VREA ++F     ++RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T++I +D+ + E+C+ + LWVDY N+  +++  S IY+DDGLISL+V + G+DF + EVENGG+LGS K VNLPGA VDLPAVS KD +DL+FGVE NVDMVFASFIR    +  ++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I  EAE   +  + FE +    +   D   + A+ AVEA+ +C A+A+IV+T +GRSAHL+++YRP  PI+ VTR+  TARQ+HL+RG+ P+ Y E     W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    1 LPGVNMSEAGSAFIQTQQLHAAMADTFLEHMCRLDIDSEPIVARNTGIICTIGPASRSVEILKEMIKSGMNIARLNFSHGTHEYHAGTIKNVREATESFSSNPIHYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLRITLDDAFQEKCDENILWVDYKNLTKVVKPGSKIYVDDGLISLLVKETGADFCLTEVENGGMLGSKKGVNLPGAAVDLPAVSTKDIQDLQFGVEQNVDMVFASFIRKAADVHAVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIALEAEAAAFHRQLFEELFRATASSKDPAEAMALGAVEASFKCLASALIVMTESGRSAHLVSRYRPRAPIIAVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDMRVNFAMDIGKARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Xenopus
Match: pkm (pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224])

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 312/524 (59.54%), Postives = 401/524 (76.53%), Query Frame = 2 
Query:  128 EPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            E  + FIQ Q++ +    + LEH  +L+ + EP V RN+GIICTIGPA +SV IL +MI +GMN+ARLNFSHGT+EYH  TIK VREA ++F     ++RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T++I +D+ + E+C+ + LWVDY N+  +++  S IY+DDGLISL+V + G+DF + EVENGG+LGS K VNLPGA VDLPAVS KD +DL+FGVE NVDMVFASFIR    +  ++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I  EAE   +  + FE +    +   D   + A+ AVEA+ +C A+A+IV+T +GRSAHL+++YRP  PI+ VTR+  TARQ+HL+RG+ P+ Y E     W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    3 EAGSAFIQTQQLHAAMADTFLEHMCRLDIDSEPIVARNTGIICTIGPASRSVEILKEMIKSGMNIARLNFSHGTHEYHAGTIKNVREATESFSSNPIHYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLRITLDDAFQEKCDENILWVDYKNLTKVVKPGSKIYVDDGLISLLVKETGADFCLTEVENGGMLGSKKGVNLPGAAVDLPAVSTKDIQDLQFGVEQNVDMVFASFIRKAADVHAVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIALEAEAAAFHRQLFEELFRATASSKDPAEAMALGAVEASFKCLASALIVMTESGRSAHLVSRYRPRAPIIAVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDMRVNFAMDIGKARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 526
[Close]
BLAST of Pyruvate kinase vs. Ensembl Mouse
Match: Pkm (pyruvate kinase, muscle [Source:MGI Symbol;Acc:MGI:97591])

HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0
Identity = 321/526 (61.03%), Postives = 402/526 (76.43%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV +L +MI +GMNVARLNFSHGT+EYH ETIK VREA ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR    + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++    FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +  +  W ED+D R++ ++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRLLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Mouse
Match: Pkm (pyruvate kinase, muscle [Source:MGI Symbol;Acc:MGI:97591])

HSP 1 Score: 659.062 bits (1699), Expect = 0.000e+0
Identity = 322/526 (61.22%), Postives = 403/526 (76.62%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV +L +MI +GMNVARLNFSHGT+EYH ETIK VREA ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR    + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  +Y  + FE ++      +D T + AV AVEA+ +C + AIIV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +  +  W ED+D R++ ++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. Ensembl Mouse
Match: Pklr (pyruvate kinase liver and red blood cell [Source:MGI Symbol;Acc:MGI:97604])

HSP 1 Score: 612.838 bits (1579), Expect = 0.000e+0
Identity = 294/519 (56.65%), Postives = 378/519 (72.83%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPY---FRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            F Q Q++ +    + LEH   L+ + EP   R++ II TIGPA +SV  L +MI AGMN+ARLNFSHG++EYH E+I  +REA ++F      +RP+AIALDTKGPEIRTG++ G    EV + KGS + + VD K+  + ++  +WVDY NI  ++ V   IYIDDGLISLVV K G + L+ EVE+GG LG+ K VNLP A+VDLP +SE+D  DLRFGVEHNVD++FASF+R  + +  ++D LG +G+ I IISKIEN EGV+KFDEILE+SDGIMVARGDLGIEIP EKV LAQKMMIGRCN AGKPV+CATQMLESMI K  PT+AE SDVANAVLDGADC+MLSGETAKG +P+EAV++ H+I REAE  +Y  + FE ++       D T  TA+ AVEA+ +C AAAIIV+T TGRSA L+++YRP   ++ VTR    ARQ HL RG+ P+ Y EP    W +D+D R+ + +  G  RGFL++ D VI+VTGW+ G G TN +R++ I
Sbjct:   24 FFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREAAESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFRTRGDAKTVWVDYHNITQVVAVGGRIYIDDGLISLVVRKIGPEGLVTEVEHGGFLGNRKGVNLPNAEVDLPGLSEQDLLDLRFGVEHNVDIIFASFVRKASDVVAVRDALGPEGRGIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAVWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLTI 542
[Close]
BLAST of Pyruvate kinase vs. Ensembl Mouse
Match: Pklr (pyruvate kinase liver and red blood cell [Source:MGI Symbol;Acc:MGI:97604])

HSP 1 Score: 612.453 bits (1578), Expect = 0.000e+0
Identity = 294/519 (56.65%), Postives = 378/519 (72.83%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPY---FRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            F Q Q++ +    + LEH   L+ + EP   R++ II TIGPA +SV  L +MI AGMN+ARLNFSHG++EYH E+I  +REA ++F      +RP+AIALDTKGPEIRTG++ G    EV + KGS + + VD K+  + ++  +WVDY NI  ++ V   IYIDDGLISLVV K G + L+ EVE+GG LG+ K VNLP A+VDLP +SE+D  DLRFGVEHNVD++FASF+R  + +  ++D LG +G+ I IISKIEN EGV+KFDEILE+SDGIMVARGDLGIEIP EKV LAQKMMIGRCN AGKPV+CATQMLESMI K  PT+AE SDVANAVLDGADC+MLSGETAKG +P+EAV++ H+I REAE  +Y  + FE ++       D T  TA+ AVEA+ +C AAAIIV+T TGRSA L+++YRP   ++ VTR    ARQ HL RG+ P+ Y EP    W +D+D R+ + +  G  RGFL++ D VI+VTGW+ G G TN +R++ I
Sbjct:   55 FFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREAAESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFRTRGDAKTVWVDYHNITQVVAVGGRIYIDDGLISLVVRKIGPEGLVTEVEHGGFLGNRKGVNLPNAEVDLPGLSEQDLLDLRFGVEHNVDIIFASFVRKASDVVAVRDALGPEGRGIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAVWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLTI 573
[Close]
BLAST of Pyruvate kinase vs. Ensembl Mouse
Match: Pkm (pyruvate kinase, muscle [Source:MGI Symbol;Acc:MGI:97591])

HSP 1 Score: 283.878 bits (725), Expect = 7.930e-92
Identity = 137/228 (60.09%), Postives = 172/228 (75.44%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFG 796
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV +L +MI +GMNVARLNFSHGT+EYH ETIK VREA ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FG
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFG 232
[Close]
BLAST of Pyruvate kinase vs. UniProt/SwissProt
Match: sp|P14618|KPYM_HUMAN (Pyruvate kinase PKM OS=Homo sapiens OX=9606 GN=PKM PE=1 SV=4)

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 323/526 (61.41%), Postives = 405/526 (77.00%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  +Y  + FE ++      +D T +TAV AVEA+ +C + AIIV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. UniProt/SwissProt
Match: sp|Q5NVN0|KPYM_PONAB (Pyruvate kinase PKM OS=Pongo abelii OX=9601 GN=PKM PE=2 SV=3)

HSP 1 Score: 674.085 bits (1738), Expect = 0.000e+0
Identity = 321/526 (61.03%), Postives = 403/526 (76.62%), Query Frame = 2 
Query:  122 YFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE S VANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++  K FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +P    W ED+D R++++++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    5 HSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLLTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSGVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. UniProt/SwissProt
Match: sp|P00548|KPYM_CHICK (Pyruvate kinase PKM OS=Gallus gallus OX=9031 GN=PKM PE=2 SV=2)

HSP 1 Score: 674.085 bits (1738), Expect = 0.000e+0
Identity = 322/531 (60.64%), Postives = 408/531 (76.84%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAI-KHTI--SGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            SK+ +    FIQ Q++ +    + LEH  +L+ + EP + RN+GIICTIGPA +SV  L +MI +GMNVARLNFSHGT+EYHE TIK VREA ++F      +RP+AIALDTKGPEIRTGL+ GS T EV L KG+ +K+ +DN + E C+ + LWVDY N+I +++V S IY+DDGLISL+V +KG DF+M EVENGG+LGS K VNLPGA VDLPAVSEKD +DL+FGVE NVDMVFASFIR    +  ++ +LGEKGKHI IISKIEN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I REAE  ++  + FE I +H++    P D   + A  AVEA+ +C AAA+IV+T +GRSAHL+++YRP  PI+ VTR+  TARQ+HL+RG+ P+   +P    W ED+D R++  ++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    2 SKHHDAGTAFIQTQQLHAAMADTFLEHMCRLDIDSEPTIARNTGIICTIGPASRSVDKLKEMIKSGMNVARLNFSHGTHEYHEGTIKNVREATESFASDPITYRPVAIALDTKGPEIRTGLIKGSGTAEVELKKGAALKVTLDNAFMENCDENVLWVDYKNLIKVIDVGSKIYVDDGLISLLVKEKGKDFVMTEVENGGMLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQNVDMVFASFIRKAADVHAVRKVLGEKGKHIKIISKIENHEGVRRFDEIMEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPIICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIAREAEAAMFHRQQFEEILRHSVHHREPAD---AMAAGAVEASFKCLAAALIVMTESGRSAHLVSRYRPRAPIIAVTRNDQTARQAHLYRGVFPVLCKQPAHDAWAEDVDLRVNLGMNVGKARGFFKTGDLVIVLTGWRPGSGYTNTMRVVPV 529
[Close]
BLAST of Pyruvate kinase vs. UniProt/SwissProt
Match: sp|P11979|KPYM_FELCA (Pyruvate kinase PKM OS=Felis catus OX=9685 GN=PKM PE=1 SV=2)

HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 320/519 (61.66%), Postives = 402/519 (77.46%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV IL +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S +Y+DDGLISL+V +KG+DFL+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR  + + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++  K FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+  TARQ+HL+RG+ P+   +P    W ED+D R++ +++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   12 FIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVEILKEMIKSGMNVARLNFSHGTHEYHAETIKNVRAATESFASDPIRYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENVLWLDYKNICKVVEVGSKVYVDDGLISLLVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRGSSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEAWAEDVDLRVNLAMNVGKARGFFKHGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. UniProt/SwissProt
Match: sp|P11980|KPYM_RAT (Pyruvate kinase PKM OS=Rattus norvegicus OX=10116 GN=Pkm PE=1 SV=3)

HSP 1 Score: 669.463 bits (1726), Expect = 0.000e+0
Identity = 319/524 (60.88%), Postives = 401/524 (76.53%), Query Frame = 2 
Query:  128 EPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            E    FIQ Q++ +    + LEH  +L+ +  P   RN+GIICTIGPA +SV +L +MI +GMNVARLNFSHGT+EYH ETIK VR A ++F      +RP+A+ALDTKGPEIRTGL+ GS T EV L KG+T+KI +DN Y E+C+ + LW+DY NI  ++EV S IY+DDGLISL V +KG+D+L+ EVENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE +VDMVFASFIR    + E++ +LGEKGK+I IISKIEN EGV++FDEILE SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H I REAE  ++    FE +    S  TD   + A+ +VEA+ +C AAA+IV+T +GRSAH +A+YRP  PI+ VTR+P TARQ+HL+RG+ P+   +  +  W ED+D R++ +++ G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:    7 EAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRAATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAVFHRLLFEELARASSQSTDPLEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
[Close]
BLAST of Pyruvate kinase vs. TrEMBL
Match: A0A267EYV5 (Pyruvate kinase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019710g1 PE=3 SV=1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 346/526 (65.78%), Postives = 431/526 (81.94%), Query Frame = 2 
Query:  137 NYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSG 1714
             YFIQ Q++ +++ RS+LEH   L+ + EP  +RN+GIICTIGPACKSV ILHKMI+ GMN+AR+NFSHGT+EYH +T++LVR+A +TFKP+ RP+ IALDTKGPEIRTGL+ GS T EV L +G  I + +D+ ++E C+   LWVDY NI+ ++     ++IDDGLISL+V +     L+CEVENGG LGS K VNLPG+ VDLPAVSEKD+EDL+F V+  +DMVFASFIR+ +AI+EI+ ILGE GK+I II+KIEN EGV++FDEI+E +DGIMVARGDLGIEIPPEKV LAQKMMIGRCNRAGKPVICATQMLESMI+KP PT+AEISDVANAVLDGADCVMLSGETAKG +PLEAV+ MH +CREAE+ +Y S+ FE +K  +  PTD TH+TA+AA EAA RC AAAIIVITTTGRSA L+A++RP CPILTV+R   T RQ+HLWRGLHP+F++E R   WTEDMD+RI ++++YG +RGF+   DFV++VTGWKAG G TNTLR+I++ E+   SG
Sbjct:   22 QYFIQKQQLQASNARSHLEHLCHLDVDSEPAYVRNTGIICTIGPACKSVAILHKMIANGMNIARMNFSHGTHEYHRQTVELVRQAAETFKPWSRPVGIALDTKGPEIRTGLIGGSGTAEVELVRGEPITVTIDSAFAENCSKDTLWVDYPNIVKVINEGGRVFIDDGLISLLVKEIRDKSLLCEVENGGKLGSKKGVNLPGSPVDLPAVSEKDREDLKFAVDLGLDMVFASFIRNADAIREIRHILGEAGKNIKIIAKIENHEGVRRFDEIMEAADGIMVARGDLGIEIPPEKVFLAQKMMIGRCNRAGKPVICATQMLESMISKPRPTRAEISDVANAVLDGADCVMLSGETAKGDFPLEAVRTMHQVCREAESAMYTSQLFEDLKAELLEPTDITHTTAIAASEAANRCRAAAIIVITTTGRSAQLVARHRPRCPILTVSRLAQTCRQAHLWRGLHPLFFDETRSDQWTEDMDKRIEFAIAYGRQRGFINRGDFVVMVTGWKAGTGFTNTLRVIQVTEDGEGSG 547
[Close]
BLAST of Pyruvate kinase vs. TrEMBL
Match: A0A267F274 (Pyruvate kinase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009984g1 PE=3 SV=1)

HSP 1 Score: 754.592 bits (1947), Expect = 0.000e+0
Identity = 354/533 (66.42%), Postives = 434/533 (81.43%), Query Frame = 2 
Query:  140 YFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQS--GVRQIVS 1732
            YFIQ Q++ +++ RS+LEH  +L+ + +P  +RN+GIICTIGPACKSV +LHKMI+AGMN+AR+NFSHGT+EYH+ET++LVR+A DTFK + RP+AIALDTKGPEIRTGL+ GS T EV L +GS I +  D  +SE C++  LWVDY NI+ ++     +++DDGLISL+V +   + L+CEVENGG LGS K VNLPGA VDLPAVSEKDK DLRF VE  +D+VFASFIR+  AI+EIK ILGE+GK+I II+KIEN EGV++FDEI+E +DGIMVARGDLGIEIPPEKV LAQKMMIGRCNRAGKPVICATQMLESMI KP PT+AEISDVANAVLDGADCVMLSGETAKG YPLEAV+ MH +CREAE+ ++ S+ FE +K  +  PTD TH+TA+AA EAA RC AAAIIVITTTGRSA LI+++RP CPILTV+R   T RQ+HL+RGLHP+FY+ PR   WTEDMD+RI +++ YG +R F+ + DFV+IVTGWKAG G TNTLRI+++ E    S  G   IVS
Sbjct:   15 YFIQKQQLQASNARSHLEHLCRLDIDLDPAYVRNTGIICTIGPACKSVAVLHKMIAAGMNIARMNFSHGTHEYHKETVELVRQAADTFKGWSRPVAIALDTKGPEIRTGLIGGSGTAEVELKRGSEIVVTTDPAHSENCSADMLWVDYPNIVKVVREGGRVFVDDGLISLLVKEVRDNSLLCEVENGGNLGSKKGVNLPGAPVDLPAVSEKDKSDLRFAVEMGLDIVFASFIRNAEAIREIKHILGEEGKNIKIIAKIENHEGVRRFDEIMEAADGIMVARGDLGIEIPPEKVFLAQKMMIGRCNRAGKPVICATQMLESMITKPRPTRAEISDVANAVLDGADCVMLSGETAKGSYPLEAVKTMHQVCREAESAMFSSQLFEDLKAELLEPTDITHTTAIAASEAANRCRAAAIIVITTTGRSASLISRHRPRCPILTVSRLAQTCRQAHLFRGLHPLFYDAPRCEQWTEDMDKRIDFAIDYGRKREFINVGDFVVIVTGWKAGAGFTNTLRIVQVTEQAASSYIGAPNIVS 547
[Close]
BLAST of Pyruvate kinase vs. TrEMBL
Match: Q5DAM7 (Pyruvate kinase OS=Schistosoma japonicum OX=6182 GN=EWB00_008759 PE=2 SV=1)

HSP 1 Score: 739.184 bits (1907), Expect = 0.000e+0
Identity = 348/527 (66.03%), Postives = 421/527 (79.89%), Query Frame = 2 
Query:  179 RSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISEFED 1759
            RS LEH   L+ + +P  +RN+GIICTIGPAC++V +L  M++AGMN+ARLNFSHG++EYH ETIKLVREA  T KP+ RPI IALDTKGPEIRTGL+NGS T EV+L  G  I++  D+ Y E CN S L+VDY NI+++L   S I++DDGLISLVV+ KG ++L CEVENGG LGS K VNLPGA VDLPAVSEKDK+DLRF VEHNVDMVFASFIR+  A+ EI+ +LGE G +I II+KIEN EGVQ+F+EILE+ DGIMVARGDLGIEIP EKV +AQKMMIGRCN+ GKPVICATQMLESM  KP PT+AE SDVANAVLDGADCVMLSGETAKG YPLE VQ MH IC +AE  ++  + FE +K ++ GPT+  H+TA+AAVEAA RC+AAAIIVITT+GRS  LI+++RP CPILTVTRH + ARQ HL+RG+HP+FY E R  +W EDMD RI Y++ YG +R FL    FVIIVTGWKAG GSTNTLR++++E+ +     + IV VP I+ F+D
Sbjct:   39 RSLLEHVVSLDIDHQPTFVRNTGIICTIGPACRTVEMLQNMMTAGMNIARLNFSHGSHEYHAETIKLVREAAATLKPFPRPIGIALDTKGPEIRTGLINGSGTAEVSLEVGHKIRVTTDSAYMEACNESTLYVDYPNIVHVLSNGSKIFVDDGLISLVVLSKGPNYLECEVENGGKLGSRKGVNLPGAHVDLPAVSEKDKQDLRFAVEHNVDMVFASFIRNAGAVHEIRQLLGENGAYIKIIAKIENHEGVQRFNEILEVVDGIMVARGDLGIEIPAEKVFIAQKMMIGRCNQVGKPVICATQMLESMTTKPRPTRAESSDVANAVLDGADCVMLSGETAKGLYPLETVQTMHRICVQAEAAMFHGQLFEDLKSSLCGPTEMAHTTAIAAVEAASRCNAAAIIVITTSGRSCQLISRHRPRCPILTVTRHEVIARQIHLYRGVHPLFYGESRAGEWYEDMDRRIRYAIDYGKKRSFLTPGCFVIIVTGWKAGSGSTNTLRVVKLEDAE----TKPIVMVPSITHFDD 561
[Close]
BLAST of Pyruvate kinase vs. TrEMBL
Match: A0A3Q0KH21 (Pyruvate kinase OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 736.487 bits (1900), Expect = 0.000e+0
Identity = 344/527 (65.28%), Postives = 422/527 (80.08%), Query Frame = 2 
Query:  179 RSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISEFED 1759
            RS LEH   L+ + +P  +RN+GIICTIGPAC++V +L  MI++GMN+ARLNFSHG++EYH ETIKL+REA  T KP+ RPI IALDTKGPEIRTGL+NGS T EV+L  G  I++  D+ Y E+CN S L+VDY NI+++L   S I++DDGL+SLVV+ KG D+L CEVENGG LGS K VNLPGA VDLPAVSEKDK+DLRF VEHNVDMVFASFIR+ +A+ +I+ +LG+ G +I II+KIEN EGVQ+F+EIL++ DGIMVARGDLGIEIP EKV +AQKMMIGRCN+ GKPVICATQMLESM  KP PT+AE SDVANAVLDGADCVMLSGETAKG YPLE VQ MH IC +AE  ++  + FE +K ++ GPT+  H+TA+AAVEAA RC+AAAIIVITT+GRS  LI+++RP CPILTVTRH + ARQ HL+RG+HPI+Y E R  +W EDMD RI Y+L YG +R F     FVIIVTGWKAG GSTNTLR++++E+ +     + IV VP I+ F+D
Sbjct:   39 RSLLEHVVSLDIDHQPAYVRNTGIICTIGPACRTVEMLQNMITSGMNIARLNFSHGSHEYHAETIKLIREAASTLKPFPRPIGIALDTKGPEIRTGLINGSGTAEVSLEVGHKIRVTTDSAYMEKCNESILYVDYRNIVHVLSKGSKIFVDDGLLSLVVLDKGPDYLECEVENGGKLGSRKGVNLPGAHVDLPAVSEKDKQDLRFAVEHNVDMVFASFIRNADAVHQIRQLLGDNGAYIKIIAKIENHEGVQRFNEILDVVDGIMVARGDLGIEIPAEKVFIAQKMMIGRCNQVGKPVICATQMLESMTTKPRPTRAESSDVANAVLDGADCVMLSGETAKGLYPLETVQTMHRICIQAEAAMFHGQLFEDLKSSLYGPTEMAHTTAIAAVEAASRCNAAAIIVITTSGRSCQLISRHRPRCPILTVTRHEVIARQIHLYRGVHPIYYGESRAGEWYEDMDRRIRYALDYGKKRSFFSPGCFVIIVTGWKAGSGSTNTLRVVKLEDAE----TKPIVMVPSITHFDD 561
[Close]
BLAST of Pyruvate kinase vs. TrEMBL
Match: A0A430Q112 (Pyruvate kinase OS=Schistosoma bovis OX=6184 GN=DC041_0005495 PE=3 SV=1)

HSP 1 Score: 734.561 bits (1895), Expect = 0.000e+0
Identity = 345/527 (65.46%), Postives = 420/527 (79.70%), Query Frame = 2 
Query:  179 RSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISEFED 1759
            RS LEH   L+ + +P  +RN+GIICTIGPAC++V IL  M+++GMN+ARLNFSHG++EYH +TI+LVREA  T KP+ RPI IALDTKGPEIRTGL+NGS T EVNL  G  I++  D  Y E CN S L+VDY NI+++L   S I++DDGLISLVV+ KG D+L CEVENGG LGS K VNLPGA VDL AVSEKDK+DLRF VEHNVDMVFASFIR+ +A+ +I+ +LG+ G HI II+KIEN EGVQ+F+EIL+ +DGIMVARGDLGIEIP EKV +AQKMMIGRCN+ GKPVICATQMLESM  KP PT+AE SDVANAVLDGADCVMLSGETAKG YPLEAVQ MH IC +AE  ++  + FE +K ++ GPT+  H+TA+AAVEAA RC+AAAIIVITT+GRS  LI+++RP CPILTVTRH   ARQ HL+RG+HP++Y EPR  +W EDMD RI Y++ YG +R F     FVIIVTGWKAG GSTNTLR++++E+ +     + IV VP I+ F+D
Sbjct:   42 RSLLEHVVSLDIDHQPAYVRNTGIICTIGPACRTVEILQNMMTSGMNIARLNFSHGSHEYHAKTIELVREAAATLKPFPRPIGIALDTKGPEIRTGLINGSGTAEVNLQVGHKIRVTTDCAYMEACNESILYVDYRNIVHVLSKGSKIFVDDGLISLVVLDKGPDYLECEVENGGKLGSRKGVNLPGAHVDLDAVSEKDKQDLRFAVEHNVDMVFASFIRNADAVHQIRQLLGDNGSHIKIIAKIENHEGVQRFNEILDAADGIMVARGDLGIEIPAEKVFIAQKMMIGRCNQVGKPVICATQMLESMTTKPRPTRAESSDVANAVLDGADCVMLSGETAKGLYPLEAVQTMHRICVQAEAAMFHGQLFEDLKSSLYGPTEMAHTTAIAAVEAASRCNAAAIIVITTSGRSCQLISRHRPRCPILTVTRHEYIARQIHLYRGVHPLYYGEPRAGEWYEDMDRRIRYAIDYGRKRSFFSPGCFVIIVTGWKAGSGSTNTLRVVKLEDAE----TKPIVMVPSITHFDD 564
[Close]
BLAST of Pyruvate kinase vs. Ensembl Cavefish
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:103044212])

HSP 1 Score: 672.544 bits (1734), Expect = 0.000e+0
Identity = 316/528 (59.85%), Postives = 406/528 (76.89%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            SK  +  + FIQ Q++ +    + LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+ARLNFSHG++EYH ETIK +REA ++F+P   ++RPI IALDTKGPEIRTGL+ GS T EV L KG+TIK+ +D+ Y E C+   LW+DY NI  ++E+ S +YIDDGLISL V + GSD+++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    + E++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++       D + + A+ AVE++ +C A+AIIV+T +GRSAHLI++YRP  PI+ VTR+  TARQ+HL+RG+ P+FYN+P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   27 SKPQDMGSAFIQTQQLNAAMADTFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVDMLKEMIKSGMNIARLNFSHGSHEYHGETIKNIREATESFEPGSIHYRPIGIALDTKGPEIRTGLIKGSGTAEVELKKGNTIKVTLDDAYMENCDEDVLWLDYKNITKVVEIGSKVYIDDGLISLQVQEIGSDYILCEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMVFASFIRKAQDVNEVRQVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQLFEELRRANKMTHDPSEAVAIGAVESSFKCCASAIIVLTKSGRSAHLISRYRPRAPIIAVTRNGQTARQAHLYRGIFPVFYNKPAHDVWAEDVDLRVNFAMDVGKARGFFKTGDVVIVLTGWRPGSGFTNTMRVVPV 554
[Close]
BLAST of Pyruvate kinase vs. Ensembl Cavefish
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:103044212])

HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0
Identity = 316/528 (59.85%), Postives = 406/528 (76.89%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            SK  +  + FIQ Q++ +    + LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+ARLNFSHG++EYH ETIK +REA ++F+P   ++RPI IALDTKGPEIRTGL+ GS T EV L KG+TIK+ +D+ Y E C+   LW+DY NI  ++E+ S +YIDDGLISL V + GSD+++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    + E++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++       D + + A+ AVE++ +C A+AIIV+T +GRSAHLI++YRP  PI+ VTR+  TARQ+HL+RG+ P+FYN+P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   29 SKPQDMGSAFIQTQQLNAAMADTFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVDMLKEMIKSGMNIARLNFSHGSHEYHGETIKNIREATESFEPGSIHYRPIGIALDTKGPEIRTGLIKGSGTAEVELKKGNTIKVTLDDAYMENCDEDVLWLDYKNITKVVEIGSKVYIDDGLISLQVQEIGSDYILCEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMVFASFIRKAQDVNEVRQVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQLFEELRRANKMTHDPSEAVAIGAVESSFKCCASAIIVLTKSGRSAHLISRYRPRAPIIAVTRNGQTARQAHLYRGIFPVFYNKPAHDVWAEDVDLRVNFAMDVGKARGFFKTGDVVIVLTGWRPGSGFTNTMRVVPV 556
[Close]
BLAST of Pyruvate kinase vs. Ensembl Cavefish
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:103044212])

HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0
Identity = 316/528 (59.85%), Postives = 406/528 (76.89%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            SK  +  + FIQ Q++ +    + LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+ARLNFSHG++EYH ETIK +REA ++F+P   ++RPI IALDTKGPEIRTGL+ GS T EV L KG+TIK+ +D+ Y E C+   LW+DY NI  ++E+ S +YIDDGLISL V + GSD+++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    + E++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++       D + + A+ AVE++ +C A+AIIV+T +GRSAHLI++YRP  PI+ VTR+  TARQ+HL+RG+ P+FYN+P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:  102 SKPQDMGSAFIQTQQLNAAMADTFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVDMLKEMIKSGMNIARLNFSHGSHEYHGETIKNIREATESFEPGSIHYRPIGIALDTKGPEIRTGLIKGSGTAEVELKKGNTIKVTLDDAYMENCDEDVLWLDYKNITKVVEIGSKVYIDDGLISLQVQEIGSDYILCEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMVFASFIRKAQDVNEVRQVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQLFEELRRANKMTHDPSEAVAIGAVESSFKCCASAIIVLTKSGRSAHLISRYRPRAPIIAVTRNGQTARQAHLYRGIFPVFYNKPAHDVWAEDVDLRVNFAMDVGKARGFFKTGDVVIVLTGWRPGSGFTNTMRVVPV 629
[Close]
BLAST of Pyruvate kinase vs. Ensembl Cavefish
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:103044212])

HSP 1 Score: 671.003 bits (1730), Expect = 0.000e+0
Identity = 314/519 (60.50%), Postives = 402/519 (77.46%), Query Frame = 2 
Query:  143 FIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            FIQ Q++ +    + LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+ARLNFSHG++EYH ETIK +REA ++F+P   ++RPI IALDTKGPEIRTGL+ GS T EV L KG+TIK+ +D+ Y E C+   LW+DY NI  ++E+ S +YIDDGLISL V + GSD+++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    + E++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++       D + + A+ AVE++ +C A+AIIV+T +GRSAHLI++YRP  PI+ VTR+  TARQ+HL+RG+ P+FYN+P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:   66 FIQTQQLNAAMADTFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVDMLKEMIKSGMNIARLNFSHGSHEYHGETIKNIREATESFEPGSIHYRPIGIALDTKGPEIRTGLIKGSGTAEVELKKGNTIKVTLDDAYMENCDEDVLWLDYKNITKVVEIGSKVYIDDGLISLQVQEIGSDYILCEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMVFASFIRKAQDVNEVRQVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQLFEELRRANKMTHDPSEAVAIGAVESSFKCCASAIIVLTKSGRSAHLISRYRPRAPIIAVTRNGQTARQAHLYRGIFPVFYNKPAHDVWAEDVDLRVNFAMDVGKARGFFKTGDVVIVLTGWRPGSGFTNTMRVVPV 584
[Close]
BLAST of Pyruvate kinase vs. Ensembl Cavefish
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:103044212])

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 316/528 (59.85%), Postives = 403/528 (76.33%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            SK  +  + FIQ Q++ +    + LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+ARLNFSHG++EYH ETIK +REA ++F+P   ++RPI IALDTKGPEIRTGL+ GS T EV L KG+TIK+ +D+ Y E C+   LW+DY NI  ++E+ S +YIDDGLISL V + GSD+++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    + E++ +LGEKGK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++      TD   + A+ AVEA+ +  A A IV+T +GRSAHLI++YRP  PI+ VTR+  TARQ+HL+RG+ P+FYN+P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R++ +
Sbjct:  102 SKPQDMGSAFIQTQQLNAAMADTFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVDMLKEMIKSGMNIARLNFSHGSHEYHGETIKNIREATESFEPGSIHYRPIGIALDTKGPEIRTGLIKGSGTAEVELKKGNTIKVTLDDAYMENCDEDVLWLDYKNITKVVEIGSKVYIDDGLISLQVQEIGSDYILCEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIKDLQFGVEMGVDMVFASFIRKAQDVNEVRQVLGEKGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQVFEDLRRCTPHSTDPAEAIAIGAVEASFKLLAPAFIVLTGSGRSAHLISRYRPRAPIIAVTRNGQTARQAHLYRGIFPVFYNKPAHDVWAEDVDLRVNFAMDVGKARGFFKTGDVVIVLTGWRPGSGFTNTMRVVPV 629
[Close]
BLAST of Pyruvate kinase vs. Ensembl Sea Lamprey
Match: pkma (pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-031201-4])

HSP 1 Score: 649.047 bits (1673), Expect = 0.000e+0
Identity = 307/533 (57.60%), Postives = 402/533 (75.42%), Query Frame = 2 
Query:  128 EPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNG-------SATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVV--IKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            +P + F+Q Q+++++   + LEH   L+ + EP   RN+GIICTIGPA +S+  L +MI +GMN+AR+NFSHG++EYH +TIK VREA ++F     Y+RP+ +ALDTKGPEIRTGL+ G       S T EV L KG+ +K+ +D+ Y + C+ + +W+DY NI  ++   S I++DDGLISL+V  I KG D+++ EVENGG+LGS K VNLPGA VDLPAVS KD +DL+FGV+  VDMVFASFIR    ++E++ +LGEKGK+I IISKIEN EGV++F+EILE SDGIMVARGDLGIEIP EKV +AQKMMIGRCNRAGKPVICATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV++ H+I REAE  +Y  + FE ++      +D T +TA+  VEA+ +C A AIIV+T +GRSAHL++KYRP  PI+ VTR   TARQ+HL+RG+ P+   EP    W ED+D R++ ++  G  RGF + +D VI+VTGW+ G G TN +R+I++
Sbjct:    7 DPGSAFVQTQQLSASRADTFLEHMCLLDIDSEPITARNTGIICTIGPASRSLDTLKEMIKSGMNIARMNFSHGSHEYHSDTIKNVREATESFAGNPIYYRPVGVALDTKGPEIRTGLIKGAVVVAAQSGTAEVELKKGAELKVTLDDAYQDACDDTMVWLDYKNITKVVTPGSKIFVDDGLISLLVKEIGKGDDYVITEVENGGMLGSRKGVNLPGAAVDLPAVSAKDVQDLQFGVDQGVDMVFASFIRKAADVEEVRAVLGEKGKNIKIISKIENHEGVRRFEEILEASDGIMVARGDLGIEIPGEKVFIAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAESSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHAIAREAEAAMYHRQLFEELRRLSGITSDPTETTAIGTVEASFKCSAGAIIVLTKSGRSAHLLSKYRPRAPIIAVTRCGQTARQAHLYRGIFPVLCREPVNDVWAEDVDLRVNLAMEVGKCRGFFKQDDVVIVVTGWRPGSGYTNMMRVIKV 539
[Close]
BLAST of Pyruvate kinase vs. Ensembl Yeast
Match: CDC19 (Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication [Source:SGD;Acc:S000000036])

HSP 1 Score: 452.21 bits (1162), Expect = 1.285e-154
Identity = 232/486 (47.74%), Postives = 323/486 (66.46%), Query Frame = 2 
Query:  233 IRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVN--LYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSD-FLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRI 1681
            +R + II TIGP   +   L  +  AG+N+ R+NFSHG+YEYH+  I   R++ + +    RP+AIALDTKGPEIRTG    + T +V+  +     +    D+KY++ C+   ++VDY NI  ++     IY+DDG++S  V++   D  L  +  N G + S+K VNLPG  VDLPA+SEKDKEDLRFGV++ V MVFASFIR+ N +  I+++LGE+GK + II KIEN +GV  FDEIL+++DG+MVARGDLGIEIP  +V+  QK +I + N AGKPVICATQMLESM   P PT+AE+SDV NA+LDGADCVMLSGETAKG YP+ AV  M      AE  +     ++ +++    PT  T + A +AV A     A AIIV++T+G +  L++KYRP CPI+ VTR P  AR SHL+RG+ P  + +  + DWT+D++ RI++ +      G L+  D  + + G+KAG G +NTL++
Sbjct:   18 LRRTSIIGTIGPKTNNPETLVALRKAGLNIVRMNFSHGSYEYHKSVIDNARKSEELYP--GRPLAIALDTKGPEIRTG----TTTNDVDYPIPPNHEMIFTTDDKYAKACDDKIMYVDYKNITKVISAGRIIYVDDGVLSFQVLEVVDDKTLKVKALNAGKICSHKGVNLPGTDVDLPALSEKDKEDLRFGVKNGVHMVFASFIRTANDVLTIREVLGEQGKDVKIIVKIENQQGVNNFDEILKVTDGVMVARGDLGIEIPAPEVLAVQKKLIAKSNLAGKPVICATQMLESMTYNPRPTRAEVSDVGNAILDGADCVMLSGETAKGNYPINAVTTMAETAVIAEQAIAYLPNYDDMRNCTPKPTSTTETVAASAVAAVFEQKAKAIIVLSTSGTTPRLVSKYRPNCPIILVTRCPRAARFSHLYRGVFPFVFEKEPVSDWTDDVEARINFGIEKAKEFGILKKGDTYVSIQGFKAGAGHSNTLQV 497
[Close]
BLAST of Pyruvate kinase vs. Ensembl Yeast
Match: PYK2 (Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication [Source:SGD;Acc:S000005874])

HSP 1 Score: 427.172 bits (1097), Expect = 1.200e-144
Identity = 224/492 (45.53%), Postives = 316/492 (64.23%), Query Frame = 2 
Query:  224 PGVIRNSGIICTIGP---ACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIV--VDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKK-GSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRI 1681
            P  +R + II TIGP   +C+++T L K   AG+N+ RLNFSHG+YE+H+  I+   ++   F    RP+AIALDTKGPEIRTG        + +LY     +++   D  ++   N   +++DY N+  ++     IY+DDG++S  V++      L  +  N G + S+K VNLP   VDLP +S KD +DL+FGV + + +VFASFIR+   +  I+  LG +G+ I IISKIEN +G+  FDEILE++DG+M+ARGDLGIEI   +V+  QK +I +CN AGKPVICATQML+SM + P PT+AE+SDV NAVLDGADCVMLSGETAKG YP+ AV +M +    AE+ +     ++ ++     PT  T + A AA  A       AI+V++TTG +A L++KYRP+CPI+ VTRH  TAR +HL+RG+ P  Y   R+ DW ED+  R+ + +      G +   D V+ + G+K GVG +NTLRI
Sbjct:   17 PQQLRRTSIIGTIGPKTNSCEAITALRK---AGLNIIRLNFSHGSYEFHQSVIENAVKSEQQFPG--RPLAIALDTKGPEIRTG----RTLNDQDLYIPVDHQMIFTTDASFANTSNDKIMYIDYANLTKVIVPGRFIYVDDGILSFKVLQIIDESNLRVQAVNSGYIASHKGVNLPNTDVDLPPLSAKDMKDLQFGVRNGIHIVFASFIRTSEDVLSIRKALGSEGQDIKIISKIENQQGLDNFDEILEVTDGVMIARGDLGIEILAPEVLAIQKKLIAKCNLAGKPVICATQMLDSMTHNPRPTRAEVSDVGNAVLDGADCVMLSGETAKGDYPVNAVNIMAATALIAESTIAHLALYDDLRDATPKPTSTTETVAAAATAAILEQDGKAIVVLSTTGNTARLLSKYRPSCPIILVTRHARTARIAHLYRGVFPFLYEPKRLDDWGEDVHRRLKFGVEMARSFGMVDNGDTVVSIQGFKGGVGHSNTLRI 499
[Close]
BLAST of Pyruvate kinase vs. Ensembl Yeast
Match: YAL037C-B_mRNA (Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching [Source:SGD;Acc:S000028592])

HSP 1 Score: 130.568 bits (327), Expect = 4.024e-34
Identity = 91/323 (28.17%), Postives = 159/323 (49.23%), Query Frame = -3 
Query:  737 LIFFNSNNSQCICRTDPGFPTGYNNNKIIHL*KASTKSV*QRVMNSFIHVFCPVDHARFVVKNRMQ-ASPQMRLTSSYWMASYG*YRASRAIFCNQMSRSPXXXXXXXXXCMTTLXXXXXXXSCTVSLIRRSTNCVFYCLKEFTLI*NIFRFSTNAVHNLNGFQWILTFGCFT**HNTICSV*NSIGNVTYFSFCRSWFIDHRL*HLRSTDYRLTGPITSSNHHFLC*NYFFRWNFNTQISSSNHYTVANFKYFVKFLNAFEIFNFGYYENVFSFFSENILYFFNSVA*SNERSKHHINIMFDTKT*IFFIFLRNSWQIDFGS 1702
            ++F N  + Q +  T  G  T   N  +  L      S+    +++  ++  PV   RF+ +N  +  S Q+R +SS W +         ++  NQ         + +S  +   +S +S  S          + V + +  + +  ++F   +   H+ NG  W++T G FT  HNT+ ++ +S+ ++  FS   SW + H   HL STD  LT  +  SN   L    F+ W+FNTQ++S NH TV++ + FV+ +N   +FN     +V + F+++     N V  S+ERS+ H++ + DT++ IF +  R SWQID G+
Sbjct:    2 ILFLNGRDLQSVGVTSTGLETLDGNVSVTLLQDTEFLSLFNTEVDTGFNIISPV-RDRFLFENEWEDTSVQVRESSSSWASGNQDDWTVWSVLGNQSWGGTGSGQDNDSLGLLFENSSDSGGSDGFGGGGWLWSTVSHVIVVWQVSDSLFSNDSGFSHSGNGVDWVVTLGGFTRQHNTVSTIQDSVTDIGNFSSGWSWVVSHGFQHLGSTDNWLTSQVRLSNQFLLDSQDFWGWDFNTQVTSGNHNTVSDLQDFVEVVNTLLVFNLDNDLDVLTLFTQDFSDGQNIVGGSDERSEDHVDTVLDTESQIFLVLFRQSWQIDIGT 323
[Close]
BLAST of Pyruvate kinase vs. Ensembl Nematostella
Match: EDO49963 (Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:A7RER9])

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 280/536 (52.24%), Postives = 379/536 (70.71%), Query Frame = 2 
Query:  101 MKLNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQ 1708
            M   G++     +   ++++  S+  RSNLE  S+L+ +  P  IRNSG++CTIGPA +SV I+ ++I  GMN+ARLNFSHGT+EYH ETI  +R A     P+   + IALDTKGPEIRTGL+     GE+ L KG  +K+  D    +Q ++  ++VDY NI+ ++++  ++Y+DDGLISL V++KG +FL+ EV N  LLGS K VNLP   VDLPAVSE+DK+D+ F VE  VDM+FASFIR    I EI+ +L +  K+I I++KIEN EGV++F+EILE++DGIMVARGDLGIEIP EKV LAQKMMI RCN  G PVICATQMLESM+  P PT+AE SDVANA+LDGADCVMLSGETAKG YP++AV +MH I REAE  ++  + F+ ++H + G  D   STA+AAV A+    A AII +T TGR+A ++A++RP CPI+ VTR P  ARQ HLWRG  PI Y++  + +  E+ D+R+ ++L  G + GFL +    + V+GWK+G   TNTLRI+ + + ++ 
Sbjct:    1 MSAKGAREEYLHDVLNRHKQWISSRARSNLEFRSELDIDKHPLTIRNSGVVCTIGPASRSVEIVAQLIENGMNIARLNFSHGTHEYHRETIMNIRRAALLEWPH--AVGIALDTKGPEIRTGLLKA---GEIKLEKGQKLKVSTDKAMYDQGHTECIFVDYENIVKVVQIGGTVYVDDGLISLKVLEKGDNFLITEVLNDALLGSKKGVNLPNCAVDLPAVSEQDKKDIAFAVEMEVDMIFASFIRKPEDIAEIRKLLEKHAKNIKIVAKIENHEGVRRFNEILEVADGIMVARGDLGIEIPAEKVFLAQKMMIARCNMKGVPVICATQMLESMVQNPRPTRAETSDVANAILDGADCVMLSGETAKGLYPVQAVAMMHRISREAEAAIFHKQSFDELRHNLEGYADTHISTAIAAVAASFTAGADAIICLTHTGRTAAVVARFRPRCPIVVVTRDPRVARQMHLWRGCFPIVYDQEPLDNVLEEHDQRLDFALGMGKKMGFLTIGSTFVFVSGWKSGAAHTNTLRILTLTQEKIH 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Medaka
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:101170748])

HSP 1 Score: 675.241 bits (1741), Expect = 0.000e+0
Identity = 320/531 (60.26%), Postives = 402/531 (75.71%), Query Frame = 2 
Query:  107 LNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + GSK  +  + FIQ Q++ +    S LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+AR+NFSHG++EYH ETIK VREA ++F P    +RPI IALDTKGPEIRTGL+ GS T EV L KG+TIKI +D+ Y E C+   LW+DY NI  ++E+ S IYIDDGLISL V++ GSDF++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    +  ++ +LGE+GK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE   +  + FE ++       D + + AV AVE++ +C A+AII +T TGRSAHLI++YRP  PIL VTR+  TARQ+HL+RG+ P+ Y +P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R+I +
Sbjct:   19 MPGSKSSDMGSAFIQTQQLNAAMADSFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVEMLKEMIKSGMNIARMNFSHGSHEYHAETIKNVREACESFVPGSIQYRPIGIALDTKGPEIRTGLIRGSGTAEVELKKGNTIKITLDDAYQENCSEENLWLDYKNITKVVEIGSKIYIDDGLISLQVVEIGSDFIICEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIQDLQFGVEQGVDMVFASFIRKAADVHAVRKVLGERGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAATFHRQLFEELRRHTQLTRDPSEAVAVGAVESSFKCCASAIITLTKTGRSAHLISRYRPRAPILAVTRNAQTARQAHLYRGIFPVLYTKPAHDVWAEDVDMRVNFAMEMGKARGFFKEGDVVIVLTGWRPGSGYTNTMRVILV 549
[Close]
BLAST of Pyruvate kinase vs. Ensembl Medaka
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:101170748])

HSP 1 Score: 675.241 bits (1741), Expect = 0.000e+0
Identity = 320/531 (60.26%), Postives = 402/531 (75.71%), Query Frame = 2 
Query:  107 LNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + GSK  +  + FIQ Q++ +    S LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+AR+NFSHG++EYH ETIK VREA ++F P    +RPI IALDTKGPEIRTGL+ GS T EV L KG+TIKI +D+ Y E C+   LW+DY NI  ++E+ S IYIDDGLISL V++ GSDF++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    +  ++ +LGE+GK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE   +  + FE ++       D + + AV AVE++ +C A+AII +T TGRSAHLI++YRP  PIL VTR+  TARQ+HL+RG+ P+ Y +P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R+I +
Sbjct:    1 MPGSKSSDMGSAFIQTQQLNAAMADSFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVEMLKEMIKSGMNIARMNFSHGSHEYHAETIKNVREACESFVPGSIQYRPIGIALDTKGPEIRTGLIRGSGTAEVELKKGNTIKITLDDAYQENCSEENLWLDYKNITKVVEIGSKIYIDDGLISLQVVEIGSDFIICEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIQDLQFGVEQGVDMVFASFIRKAADVHAVRKVLGERGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAATFHRQLFEELRRHTQLTRDPSEAVAVGAVESSFKCCASAIITLTKTGRSAHLISRYRPRAPILAVTRNAQTARQAHLYRGIFPVLYTKPAHDVWAEDVDMRVNFAMEMGKARGFFKEGDVVIVLTGWRPGSGYTNTMRVILV 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Medaka
Match: pkma (pyruvate kinase PKM [Source:NCBI gene;Acc:101170748])

HSP 1 Score: 673.7 bits (1737), Expect = 0.000e+0
Identity = 319/531 (60.08%), Postives = 404/531 (76.08%), Query Frame = 2 
Query:  107 LNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            + GSK  +  + FIQ Q++ +    S LEH   L+ + EP   RN+GIICTIGPA +SV +L +MI +GMN+AR+NFSHG++EYH ETIK VREA ++F P    +RPI IALDTKGPEIRTGL+ GS T EV L KG+TIKI +D+ Y E C+   LW+DY NI  ++E+ S IYIDDGLISL V++ GSDF++CE+ENGG LGS K VNLPGA VDLPAVSEKD +DL+FGVE  VDMVFASFIR    +  ++ +LGE+GK+I IISK+EN EGV++FDEI+E SDGIMVARGDLGIEIP EKV LAQKMMIGRCNRAGKP+ CATQMLESMI KP PT+AE SDVANAVLDGADC+MLSGETAKG YPLEAV+  H I REAE  ++  + FE ++ +    +D   + A+ AVEA+ +  A+AIIV+T +GRSAHLI++YRP  PIL VTR+  TARQ+HL+RG+ P+ Y +P    W ED+D R+++++  G  RGF +  D VI++TGW+ G G TNT+R+I +
Sbjct:    1 MPGSKSSDMGSAFIQTQQLNAAMADSFLEHMCLLDIDSEPTTARNTGIICTIGPASRSVEMLKEMIKSGMNIARMNFSHGSHEYHAETIKNVREACESFVPGSIQYRPIGIALDTKGPEIRTGLIRGSGTAEVELKKGNTIKITLDDAYQENCSEENLWLDYKNITKVVEIGSKIYIDDGLISLQVVEIGSDFIICEIENGGTLGSKKGVNLPGAAVDLPAVSEKDIQDLQFGVEQGVDMVFASFIRKAADVHAVRKVLGERGKNIKIISKLENHEGVRRFDEIMEASDGIMVARGDLGIEIPTEKVFLAQKMMIGRCNRAGKPITCATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRTQHMIAREAEAAMFHRQVFEDLRRSTPHCSDPAEAIAIGAVEASFKSLASAIIVLTGSGRSAHLISRYRPRAPILAVTRNAQTARQAHLYRGIFPVLYTKPAHDVWAEDVDMRVNFAMEMGKARGFFKEGDVVIVLTGWRPGSGYTNTMRVILV 531
[Close]
BLAST of Pyruvate kinase vs. Ensembl Medaka
Match: pkmb (pyruvate kinase PKM [Source:NCBI gene;Acc:101170426])

HSP 1 Score: 655.21 bits (1689), Expect = 0.000e+0
Identity = 307/528 (58.14%), Postives = 398/528 (75.38%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRI 1690
            SK  +  +  +Q Q++ +    + +EH   L+ + EP V RN+GI+CTIGPA +SV +  +MI AGMN+AR+NFSHGT+EYH ETIK VREA ++F P    +RP+AIALDTKGPEIRTGL+ GS T EV L KG  IK+ +D+KY + C+ + LWVDY NI  +L+V +++YIDDGLISL V + G+D+LMCE+ENGGLLGS K VNLPGA VDLPA+SEKD +DL+FG+E  VDMVFASFIR    ++ ++ +LGEKGK + IISK+EN EGV++FDEILE SDGIMVARGDLGIEIP EKV +AQKMM G+CNR GKP+ICATQMLESM  KP PT+AE SDVANAVLDG DC+MLSGETAKG YPLEAV   H I REAE  ++  + FE ++ T     D T S A+ AVEA+ +C A+AIIV+T +GRSA+++++YRP  PIL VTR   TARQ+HL+RG++P+ Y +     W ED+D R+++++ YG  R F +  D  I+VTGW+ G G TNT+R++ +
Sbjct:    2 SKAKDAASSIMQTQQLHAAMADTYIEHMCLLDIDSEPAVSRNTGIVCTIGPASRSVDMAKEMIKAGMNIARMNFSHGTHEYHAETIKNVREATESFGPGTVEYRPVAIALDTKGPEIRTGLIKGSGTAEVELKKGQNIKLTLDDKYKDNCDENYLWVDYKNITKVLQVGNNVYIDDGLISLKVKEVGNDYLMCEIENGGLLGSKKGVNLPGAAVDLPALSEKDIQDLQFGLEQGVDMVFASFIRKAADVQAVRKVLGEKGKDVKIISKLENHEGVRRFDEILEASDGIMVARGDLGIEIPTEKVFIAQKMMTGKCNRIGKPIICATQMLESMTKKPRPTRAEASDVANAVLDGNDCIMLSGETAKGDYPLEAVLTQHRIAREAEAAMFHRQMFEELRRTSHLTRDPTESVAIGAVEASFKCCASAIIVLTKSGRSAYMLSRYRPRAPILAVTRCGQTARQAHLYRGVYPVLYTKNANDVWAEDVDMRVNFAMEYGKYRKFFKSGDVAIVVTGWRPGSGYTNTMRVVLV 529
[Close]
BLAST of Pyruvate kinase vs. Ensembl Medaka
Match: pklr (pyruvate kinase PKM [Source:NCBI gene;Acc:101161188])

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 290/520 (55.77%), Postives = 377/520 (72.50%), Query Frame = 2 
Query:  137 NYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKP---YFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAK-VDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRII 1684
            + FIQ Q++ ++   + LEH   L+ + EP   RN+ IICTIGPA +S+  L +M+ AGMN+ARLNFSHG +EYH ETIK +REAV+T      Y+RP+AIALDTKGPEIRTGLV G    EV L KGS +++V      ++ +++ +WVDY ++  +LE    IYIDDGL++L V++ GSD++   VE GGLL S K VNLPG   + L AVSE+D+ DLRFGV H VDMVFASFIRS   +K+++  LGE G HI +ISK+E+ +GVQ F+EIL  SDG+MVARGDLGIEIP EKV +AQKMMIGRCN AGKPVICATQMLESM++ P PT+AE SDVANAVLDGADCVMLS ETAKG++PLEAV +MHSICREAE  ++  + FE ++      +D T  TA+ AVE++ +C A AIIV+T++GRS+HL+++YRP CPI+ VTR P  ARQS L RG+ P  ++      W +D+D R+ + +  G  RGF +  D VI+VTGW  G G TN +R +
Sbjct:   21 DSFIQRQQLDASMADTFLEHLCLLDIDQEPITARNTSIICTIGPASRSIQKLQEMVKAGMNIARLNFSHGCHEYHSETIKNIREAVETITSDPLYYRPVAIALDTKGPEIRTGLVKGKVDEEVQLVKGSHVRVVTAESERDKTDATMVWVDYPHLPKVLEKGRRIYIDDGLLALKVLEIGSDWVDTVVEAGGLLCSRKGVNLPGCDLIGLQAVSEQDEADLRFGVAHGVDMVFASFIRSAQDVKDVRRALGELGSHIKVISKVESRQGVQNFEEILAESDGVMVARGDLGIEIPAEKVFIAQKMMIGRCNSAGKPVICATQMLESMVSHPRPTRAESSDVANAVLDGADCVMLSAETAKGKFPLEAVAMMHSICREAEAAIFHHQLFEELRRLTPLSSDPTEVTAIGAVESSFKCCAGAIIVLTSSGRSSHLLSRYRPRCPIIAVTRSPQVARQSQLLRGVFPALFHPLPAPVWADDVDSRVKFGMDIGKARGFFKSGDMVIVVTGWIPGSGHTNIMRAV 540
[Close]
BLAST of Pyruvate kinase vs. Planmine SMEST
Match: SMESG000017675.1 (SMESG000017675.1)

HSP 1 Score: 1139.79 bits (2947), Expect = 0.000e+0
Identity = 550/553 (99.46%), Postives = 550/553 (99.46%), Query Frame = 2 
Query:  101 MKLNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISEFED 1759
            MKLNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETI LVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGS K VNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISEFED
Sbjct:    1 MKLNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETINLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSKKGVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISEFED 553
[Close]
BLAST of Pyruvate kinase vs. Planmine SMEST
Match: SMESG000052083.1 (SMESG000052083.1)

HSP 1 Score: 812.757 bits (2098), Expect = 0.000e+0
Identity = 381/554 (68.77%), Postives = 459/554 (82.85%), Query Frame = 2 
Query:  101 MKLNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEEN-QLQSGVRQIVSVPIISEFED 1759
            MK N S  FE  NYFIQNQ +     RS+LE    L+ ++EP V+R+S IICTIGPAC++V+ L KMI++GMN+AR+NFSHG++EYH ETIKLVREAV+TF PY+RP+A+ALDTKGPEIRTGL++GS TGEV L KG+ IK+V+D KY++ CN   LWVDY NI+ IL     IY+DDGLISL+V++ G D+L CEVENGG LGS K VNLPGAKVDLPAVS+KD  DL FGVE  VDMVFASFIR G A+ EI+ ILGEKGKHILIISKIEN EGV++FDEIL+ SDGIMVARGDLGIEIP EKV LAQKMMIGRCNR GKP+ICATQMLESMI KP PT+AE SDVANA+LDGADCVMLSGETAKG YPLEAVQ+MH ICREAE+ ++Q KFFE +K  I G TDQTHSTA+AAVEAAQRC A+AI+V+TTTGRSA+LI++YRP CPILTVTRHP+TARQ HLWRG+HP+ Y+  R+ +WT DMD RI+++  Y  RRGF++  D+VI+VTGWKAG GSTNT+R+I +E++   + G+  I+S+P I++FED
Sbjct:    1 MKKNPSS-FEINNYFIQNQNVHRGVSRSHLEFICNLDIDNEPDVVRSSSIICTIGPACRTVSTLKKMIASGMNIARMNFSHGSHEYHAETIKLVREAVETFTPYYRPVALALDTKGPEIRTGLIHGSGTGEVTLVKGNKIKLVIDEKYADSCNEEILWVDYKNIVKILHKGCRIYVDDGLISLIVVETGKDYLDCEVENGGNLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRAILGEKGKHILIISKIENHEGVKRFDEILQASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRLGKPIICATQMLESMITKPRPTRAESSDVANAILDGADCVMLSGETAKGAYPLEAVQIMHHICREAESAVFQGKFFEDLKGEIVGSTDQTHSTAIAAVEAAQRCRASAIVVVTTTGRSAYLISRYRPRCPILTVTRHPVTARQGHLWRGVHPLLYDAKRLDEWTTDMDNRINWAFGYAMRRGFVKNGDYVIVVTGWKAGSGSTNTMRVILLEDDANEKDGIMPILSIPSIAKFED 553
[Close]
BLAST of Pyruvate kinase vs. Planmine SMEST
Match: SMESG000017671.1 (SMESG000017671.1)

HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0
Identity = 366/552 (66.30%), Postives = 463/552 (83.88%), Query Frame = 2 
Query:  107 LNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQS-GVRQIVSVPIISEFED 1759
            + G++YFE +NYF Q +R    DY +NLE  + LN + +PGVIRN+GIICT+GPAC SV ILHKMI+AGMNVARLNFSHGT+E H  +IKL+REA+ T KPY  PIAIALDTKGPEIRTGL+N + TGEV L KG+ IK+VV  +Y  +C+ + +WVDY +I+ +L+V + I+IDDGLISL+V +K S+FL+C +ENGG+LGS K VNLPG  VDLPAVSEKDKEDL+F VE NVD+VFASFIR+  ++  I+ ILGEKGKHILIISKIEN EG+ +FDEILE SDGIMVARGDLGIEIPPEKV+ AQKMMIG+CN AGK VICAT MLESMI K  PT+AEISDVANAV+DG+DCVMLSGE+AKG+YP+EAV++MH+ICREAE++ +QS FFE+IK T++ PTD THSTA+AAVEAAQRC+AA IIV+TT+G SA LIAKYRP+CPILT+TR+PITARQ+HLWRGLHPI +++PR  +W +DMD RI++S++Y ++RGF++  D +IIVTG KAG GSTNTL+++++E ++ +  G+++ +S P I EF+D
Sbjct:    2 MQGARYFEQSNYFFQERRRVHKDYATNLELVADLNIDSDPGVIRNTGIICTLGPACASVAILHKMIAAGMNVARLNFSHGTHEDHARSIKLLREAIATLKPYRFPIAIALDTKGPEIRTGLINNNETGEVRLIKGNNIKLVVSEEYYRKCDENFIWVDYKSILKLLKVGNRIFIDDGLISLIVEEKDSEFLLCRIENGGMLGSRKGVNLPGVNVDLPAVSEKDKEDLKFAVEQNVDIVFASFIRNAESVNIIRKILGEKGKHILIISKIENQEGMIQFDEILEASDGIMVARGDLGIEIPPEKVICAQKMMIGKCNVAGKSVICATHMLESMITKSRPTRAEISDVANAVIDGSDCVMLSGESAKGRYPVEAVKMMHTICREAEHLFFQSNFFESIKETVNLPTDHTHSTAIAAVEAAQRCNAAVIIVLTTSGTSAKLIAKYRPSCPILTLTRNPITARQAHLWRGLHPILFSDPRKDEWPKDMDLRINFSINYAYQRGFVKALDIIIIVTGSKAGSGSTNTLQVVKLEIDEKEEIGLKKYISYPTIKEFDD 553
[Close]
BLAST of Pyruvate kinase vs. Planmine SMEST
Match: SMESG000017671.1 (SMESG000017671.1)

HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0
Identity = 366/552 (66.30%), Postives = 463/552 (83.88%), Query Frame = 2 
Query:  107 LNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQS-GVRQIVSVPIISEFED 1759
            + G++YFE +NYF Q +R    DY +NLE  + LN + +PGVIRN+GIICT+GPAC SV ILHKMI+AGMNVARLNFSHGT+E H  +IKL+REA+ T KPY  PIAIALDTKGPEIRTGL+N + TGEV L KG+ IK+VV  +Y  +C+ + +WVDY +I+ +L+V + I+IDDGLISL+V +K S+FL+C +ENGG+LGS K VNLPG  VDLPAVSEKDKEDL+F VE NVD+VFASFIR+  ++  I+ ILGEKGKHILIISKIEN EG+ +FDEILE SDGIMVARGDLGIEIPPEKV+ AQKMMIG+CN AGK VICAT MLESMI K  PT+AEISDVANAV+DG+DCVMLSGE+AKG+YP+EAV++MH+ICREAE++ +QS FFE+IK T++ PTD THSTA+AAVEAAQRC+AA IIV+TT+G SA LIAKYRP+CPILT+TR+PITARQ+HLWRGLHPI +++PR  +W +DMD RI++S++Y ++RGF++  D +IIVTG KAG GSTNTL+++++E ++ +  G+++ +S P I EF+D
Sbjct:    1 MQGARYFEQSNYFFQERRRVHKDYATNLELVADLNIDSDPGVIRNTGIICTLGPACASVAILHKMIAAGMNVARLNFSHGTHEDHARSIKLLREAIATLKPYRFPIAIALDTKGPEIRTGLINNNETGEVRLIKGNNIKLVVSEEYYRKCDENFIWVDYKSILKLLKVGNRIFIDDGLISLIVEEKDSEFLLCRIENGGMLGSRKGVNLPGVNVDLPAVSEKDKEDLKFAVEQNVDIVFASFIRNAESVNIIRKILGEKGKHILIISKIENQEGMIQFDEILEASDGIMVARGDLGIEIPPEKVICAQKMMIGKCNVAGKSVICATHMLESMITKSRPTRAEISDVANAVIDGSDCVMLSGESAKGRYPVEAVKMMHTICREAEHLFFQSNFFESIKETVNLPTDHTHSTAIAAVEAAQRCNAAVIIVLTTSGTSAKLIAKYRPSCPILTLTRNPITARQAHLWRGLHPILFSDPRKDEWPKDMDLRINFSINYAYQRGFVKALDIIIIVTGSKAGSGSTNTLQVVKLEIDEKEEIGLKKYISYPTIKEFDD 552
[Close]
BLAST of Pyruvate kinase vs. Planmine SMEST
Match: SMESG000006009.1 (SMESG000006009.1)

HSP 1 Score: 702.205 bits (1811), Expect = 0.000e+0
Identity = 332/526 (63.12%), Postives = 423/526 (80.42%), Query Frame = 2 
Query:  116 SKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGIICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDTFKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSEQCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENGGLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNAIKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIEIPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVANAVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKHTISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPILTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRRGFLQMNDFVIIVTGWKAGVGSTNTLRIIRIE 1693
            S   E ++   Q +R   N     L H   L+ + +PG +RN+GIICTIGPACKS+  LH M+S+GMNVARLNFSHGT+EYH ETIKLVREA++TFKP+ RPIAIALDTKGPEIRTGL+ GS T E+NL  G  I+IV D+ ++E+C+   LW+DY NI NIL++ + IYIDDGLISL+V  KG D+L C+VENGGLLGS K VNLPGA+VDLPAVSEKDK+DL+FGVE NVD+VFASFIR+ +AI+EI+ ILGE+GKHILII+KIEN EG+++ DEI+E +DGIMVARGD+GIEIP +KVV+AQKMMI +CN+ GKPVICATQMLESM++KP PT+AEISDVANAVLDG+DCVMLSGE+AKG +P++AV+ MH +C+EAE + +Q+K+ + IK+      +   ++A+AAVEAAQ   A+AIIV+TTTG S+HLI+KYRP CPIL VTR+ + ARQ HLWRGLHP+    PR+ +W+ED+D RI  S+ YG++ G L+  D V+IVTGWK   GSTNT+RI+ ++
Sbjct:    5 SSSVEKSDSLTQ-KRFMKNKPDCVLSHLCDLDIDSDPGSVRNTGIICTIGPACKSIKTLHGMMSSGMNVARLNFSHGTHEYHAETIKLVREAIETFKPHRRPIAIALDTKGPEIRTGLIRGSGTAEINLVDGQQIRIVTDDIFAEKCDEKNLWLDYKNITNILDIGNKIYIDDGLISLIVQAKGKDYLDCKVENGGLLGSKKGVNLPGAQVDLPAVSEKDKQDLKFGVEQNVDIVFASFIRNADAIREIRSILGEEGKHILIIAKIENHEGIKRIDEIIEEADGIMVARGDMGIEIPAQKVVVAQKMMIAKCNKQGKPVICATQMLESMVSKPRPTRAEISDVANAVLDGSDCVMLSGESAKGSFPIQAVKTMHEVCKEAEAIFFQNKYLQEIKNVNRHFNNHVEASAIAAVEAAQLSKASAIIVLTTTGNSSHLISKYRPRCPILAVTRNEVVARQCHLWRGLHPLLVEFPRLDNWSEDVDARIDCSIQYGYKSGILKKGDSVVIVTGWKPVSGSTNTIRIVNVD 529
The following BLAST results are available for this feature:
BLAST of Pyruvate kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PKM0.000e+061.41pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:... [more]
PKM0.000e+061.22pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:... [more]
PKM0.000e+061.22pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:... [more]
PKM0.000e+061.22pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:... [more]
PKM0.000e+061.22pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Pyruvate kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pyk-10.000e+057.42Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL... [more]
pyk-10.000e+057.42Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL... [more]
pyk-10.000e+056.95Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL... [more]
pyk-10.000e+056.95Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL... [more]
pyk-10.000e+057.42Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q7JL... [more]
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BLAST of Pyruvate kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PyK0.000e+058.38gene:FBgn0267385 transcript:FBtr0084213[more]
PyK0.000e+058.38gene:FBgn0267385 transcript:FBtr0084214[more]
CG29642.353e-14742.26gene:FBgn0031462 transcript:FBtr0077722[more]
CG70692.129e-13545.94gene:FBgn0038952 transcript:FBtr0084215[more]
CG73627.075e-7346.55gene:FBgn0038258 transcript:FBtr0331700[more]
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BLAST of Pyruvate kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pkma0.000e+060.31pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-03... [more]
pkma0.000e+060.31pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-03... [more]
pkma0.000e+059.92pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-03... [more]
pkmb0.000e+060.35pyruvate kinase M1/2b [Source:ZFIN;Acc:ZDB-GENE-04... [more]
pkmb0.000e+060.35pyruvate kinase M1/2b [Source:ZFIN;Acc:ZDB-GENE-04... [more]
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BLAST of Pyruvate kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pkm0.000e+060.15pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224][more]
pkm0.000e+060.11pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224][more]
pkm0.000e+060.11pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224][more]
pkm0.000e+059.13pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224][more]
pkm0.000e+059.54pyruvate kinase M1/2 [Source:NCBI gene;Acc:549224][more]
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BLAST of Pyruvate kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pkm0.000e+061.03pyruvate kinase, muscle [Source:MGI Symbol;Acc:MGI... [more]
Pkm0.000e+061.22pyruvate kinase, muscle [Source:MGI Symbol;Acc:MGI... [more]
Pklr0.000e+056.65pyruvate kinase liver and red blood cell [Source:M... [more]
Pklr0.000e+056.65pyruvate kinase liver and red blood cell [Source:M... [more]
Pkm7.930e-9260.09pyruvate kinase, muscle [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of Pyruvate kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P14618|KPYM_HUMAN0.000e+061.41Pyruvate kinase PKM OS=Homo sapiens OX=9606 GN=PKM... [more]
sp|Q5NVN0|KPYM_PONAB0.000e+061.03Pyruvate kinase PKM OS=Pongo abelii OX=9601 GN=PKM... [more]
sp|P00548|KPYM_CHICK0.000e+060.64Pyruvate kinase PKM OS=Gallus gallus OX=9031 GN=PK... [more]
sp|P11979|KPYM_FELCA0.000e+061.66Pyruvate kinase PKM OS=Felis catus OX=9685 GN=PKM ... [more]
sp|P11980|KPYM_RAT0.000e+060.88Pyruvate kinase PKM OS=Rattus norvegicus OX=10116 ... [more]
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BLAST of Pyruvate kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267EYV50.000e+065.78Pyruvate kinase (Fragment) OS=Macrostomum lignano ... [more]
A0A267F2740.000e+066.42Pyruvate kinase OS=Macrostomum lignano OX=282301 G... [more]
Q5DAM70.000e+066.03Pyruvate kinase OS=Schistosoma japonicum OX=6182 G... [more]
A0A3Q0KH210.000e+065.28Pyruvate kinase OS=Schistosoma mansoni OX=6183 PE=... [more]
A0A430Q1120.000e+065.46Pyruvate kinase OS=Schistosoma bovis OX=6184 GN=DC... [more]
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BLAST of Pyruvate kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pkma0.000e+059.85pyruvate kinase PKM [Source:NCBI gene;Acc:10304421... [more]
pkma0.000e+059.85pyruvate kinase PKM [Source:NCBI gene;Acc:10304421... [more]
pkma0.000e+059.85pyruvate kinase PKM [Source:NCBI gene;Acc:10304421... [more]
pkma0.000e+060.50pyruvate kinase PKM [Source:NCBI gene;Acc:10304421... [more]
pkma0.000e+059.85pyruvate kinase PKM [Source:NCBI gene;Acc:10304421... [more]
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BLAST of Pyruvate kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pkma0.000e+057.60pyruvate kinase M1/2a [Source:ZFIN;Acc:ZDB-GENE-03... [more]
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BLAST of Pyruvate kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
CDC191.285e-15447.74Pyruvate kinase; functions as a homotetramer in gl... [more]
PYK21.200e-14445.53Pyruvate kinase; appears to be modulated by phosph... [more]
YAL037C-B_mRNA4.024e-3428.17Dubious open reading frame; unlikely to encode a f... [more]
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BLAST of Pyruvate kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO499630.000e+052.24Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:A7RE... [more]
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BLAST of Pyruvate kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pkma0.000e+060.26pyruvate kinase PKM [Source:NCBI gene;Acc:10117074... [more]
pkma0.000e+060.26pyruvate kinase PKM [Source:NCBI gene;Acc:10117074... [more]
pkma0.000e+060.08pyruvate kinase PKM [Source:NCBI gene;Acc:10117074... [more]
pkmb0.000e+058.14pyruvate kinase PKM [Source:NCBI gene;Acc:10117042... [more]
pklr0.000e+055.77pyruvate kinase PKM [Source:NCBI gene;Acc:10116118... [more]
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BLAST of Pyruvate kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017675.10.000e+099.46SMESG000017675.1[more]
SMESG000052083.10.000e+068.77SMESG000052083.1[more]
SMESG000017671.10.000e+066.30SMESG000017671.1[more]
SMESG000017671.10.000e+066.30SMESG000017671.1[more]
SMESG000006009.10.000e+063.12SMESG000006009.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030481 ID=SMED30030481|Name=Pyruvate kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=1830bp
CTAAATGAGTTTTGTTAACATCCTTTGATGAATGATTAATTTATTAAGAC
ACGGTTAGTTAAGGTTATCAAAATTATTGAAAAGTCATTTATTTATTAAA
ATGAAGTTAAATGGGTCTAAATATTTTGAGCCAACAAATTACTTTATACA
AAATCAACGAATCACAAGCAATGATTATCGATCTAATTTGGAACATACGT
CGAAGTTGAATTACAATGATGAACCAGGTGTTATAAGAAATTCTGGAATA
ATTTGTACTATTGGACCTGCATGCAAATCTGTTACAATTCTTCACAAAAT
GATTTCTGCTGGAATGAATGTTGCTCGTTTGAATTTTAGTCATGGAACTT
ATGAATATCACGAAGAAACAATTAAATTAGTAAGAGAAGCAGTTGATACA
TTTAAACCGTATTTCAGACCAATTGCTATTGCCCTCGACACAAAAGGCCC
AGAAATAAGAACTGGACTTGTAAATGGTAGTGCAACTGGCGAAGTTAATT
TGTACAAAGGATCTACAATTAAAATAGTTGTTGATAATAAATATTCTGAA
CAATGCAATAGTAGTCAACTGTGGGTAGATTATGTTAATATTATTAACAT
ACTGGAAGTAAACAGTAGCATCTACATTGACGATGGATTGATAAGCTTAG
TTGTAATCAAAAAAGGCAGCGATTTCCTTATGTGTGAAGTCGAAAATGGG
GGTTTATTGGGTTCTAACAAAAGAGTCAATCTCCCTGGAGCCAAAGTCGA
TTTGCCAGCTGTTTCTGAGAAAGATAAAGAAGATTTACGTTTTGGTGTCG
AACATAATGTTGATATGGTGTTTGCTTCTTTCATTAGATCAGGCAACGCT
ATTAAAGAAATAAAGGATATTCTCGGAGAAAAAGGAAAACACATTCTCAT
AATATCCAAAATTGAAAATCTCGAAGGCGTTCAGAAATTTGACGAAATAC
TTGAAATTAGCGACGGTATAATGGTTGCTAGAGGAGATTTGGGTATTGAA
ATTCCACCGGAAAAAGTAGTTTTAGCACAGAAAATGATGATTGGAAGATG
TAATCGGGCCGGTAAGCCTGTAATCTGTGCTACGCAAATGTTAGAGTCTA
TGATCAATAAACCAACTCCTACAAAAGCTGAAATAAGTGACGTTGCCAAT
GCTGTTCTAGACGGAGCAGATTGTGTTATGCTATCAGGTGAAACAGCCAA
AGGTCAATATCCATTGGAAGCCGTTCAAGTTATGCACAGCATTTGTCGAG
AAGCGGAAAATGTTTTATATCAAAGTAAATTCTTTGAGGCAATAAAACAC
ACAATTAGTGGACCTACGGATCAGACTCACAGTACAGCTGTTGCTGCTGT
TGAAGCTGCTCAGCGTTGTCATGCAGCAGCTATTATAGTTATAACAACAA
CTGGGAGATCTGCTCATTTGATTGCAAAATATCGCCCGACTTGCCCGATA
TTAACCGTAACTCGCCATCCAATAACTGCTCGTCAATCTCATTTGTGGAG
AGGCTTGCATCCGATTTTTTACAACGAACCGCGCATGATCGACTGGACAG
AAGACATGGATGAACGAATTCATTACTCGTTGTCATACGGATTTCGTCGA
GGCTTTCTACAAATGAATGATTTTGTTATTATTGTAACCGGTTGGAAAGC
CGGGGTCGGTTCGACAAATACATTGCGAATTATTAGAATTGAAGAAAATC
AGTTGCAATCCGGCGTGAGACAGATTGTCAGTGTTCCGATTATCTCAGAA
TTTGAAGATTAGTTGTGATTTTTTGTGTGAAAAAACAAACTAATAAATAG
CTTGCAATTTTGTTATTTTGATGATCCACA
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protein sequence of SMED30030481-orf-1

>SMED30030481-orf-1 ID=SMED30030481-orf-1|Name=SMED30030481-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=554bp
MKLNGSKYFEPTNYFIQNQRITSNDYRSNLEHTSKLNYNDEPGVIRNSGI
ICTIGPACKSVTILHKMISAGMNVARLNFSHGTYEYHEETIKLVREAVDT
FKPYFRPIAIALDTKGPEIRTGLVNGSATGEVNLYKGSTIKIVVDNKYSE
QCNSSQLWVDYVNIINILEVNSSIYIDDGLISLVVIKKGSDFLMCEVENG
GLLGSNKRVNLPGAKVDLPAVSEKDKEDLRFGVEHNVDMVFASFIRSGNA
IKEIKDILGEKGKHILIISKIENLEGVQKFDEILEISDGIMVARGDLGIE
IPPEKVVLAQKMMIGRCNRAGKPVICATQMLESMINKPTPTKAEISDVAN
AVLDGADCVMLSGETAKGQYPLEAVQVMHSICREAENVLYQSKFFEAIKH
TISGPTDQTHSTAVAAVEAAQRCHAAAIIVITTTGRSAHLIAKYRPTCPI
LTVTRHPITARQSHLWRGLHPIFYNEPRMIDWTEDMDERIHYSLSYGFRR
GFLQMNDFVIIVTGWKAGVGSTNTLRIIRIEENQLQSGVRQIVSVPIISE
FED*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036918Pyrv_Knase_C_sf
IPR040442Pyrv_Kinase-like_dom_sf
IPR001697Pyr_Knase
IPR015794Pyrv_Knase_a/b
IPR015813Pyrv/PenolPyrv_Kinase-like_dom
IPR015793Pyrv_Knase_brl
IPR015795Pyrv_Knase_C
IPR015806Pyrv_Knase_insert_dom_sf
IPR018209Pyrv_Knase_AS
IPR011037Pyrv_Knase-like_insert_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0000287magnesium ion binding
GO:0030955potassium ion binding
GO:0004743pyruvate kinase activity
GO:0003824catalytic activity
GO:0016301kinase activity
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0006096glycolytic process
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 294..318
score: 68.37
coord: 344..362
score: 80.49
coord: 106..122
score: 72.76
coord: 267..293
score: 63.93
coord: 363..379
score: 63.98
coord: 237..251
score: 48.92
coord: 319..343
score: 70.4
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 47..530
e-value: 5.9E-180
score: 596.8
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 13..530
IPR001697Pyruvate kinaseCDDcd00288Pyruvate_Kinasecoord: 45..530
e-value: 0.0
score: 712.163
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 46..393
e-value: 3.1E-156
score: 519.6
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 411..528
e-value: 1.8E-31
score: 108.8
IPR015806Pyruvate kinase, insert domain superfamilyGENE3DG3DSA:2.40.33.10coord: 117..219
e-value: 2.8E-229
score: 763.5
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3DG3DSA:3.40.1380.20coord: 29..527
e-value: 2.8E-229
score: 763.5
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILYSSF52935PK C-terminal domain-likecoord: 373..530
IPR040442Pyruvate kinase-like domain superfamilyGENE3DG3DSA:3.20.20.60coord: 46..385
e-value: 2.8E-229
score: 763.5
NoneNo IPR availablePANTHERPTHR11817:SF83PYRUVATE KINASE PKMcoord: 13..530
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 265..277
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILYSSF51621Phosphoenolpyruvate/pyruvate domaincoord: 27..393
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILYSSF50800PK beta-barrel domain-likecoord: 117..218