GSK3-beta interaction protein

Overview
NameGSK3-beta interaction protein
Smed IDSMED30030458
Length (bp)588
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GSK3-beta interaction protein (SMED30030458) t-SNE clustered cells

Violin plots show distribution of expression levels for GSK3-beta interaction protein (SMED30030458) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GSK3-beta interaction protein (SMED30030458) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GSK3-beta interaction protein (SMED30030458) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30030458

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30030458h1SMcG0001162 SmedASXL_008753SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30030458h1SMcG0001162 SmedASXL_005122SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parenchymaSMED30030458h1SMcG0001162 dd_Smed_v4_17397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30030458h1SMcG0001162 dd_Smed_v4_6352_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30030458h1SMcG0001162 dd_Smed_v6_17397_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
progenitor cellSMED30030458h1SMcG0001162 KF487101smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30030458h1SMcG0001162 KF487101smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30030458h1SMcG0001162 KF487101smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Human
Match: GSKIP (GSK3B interacting protein [Source:HGNC Symbol;Acc:HGNC:20343])

HSP 1 Score: 60.4622 bits (145), Expect = 2.314e-11
Identity = 30/98 (30.61%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+  + A   A+INV T E   +C+++T  G ++V Y +D+   ++     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVNNMFVSKSLRCADDVAYINVETKERNRYCLELTEAGLKVVGYAFDQ--VDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Human
Match: GSKIP (GSK3B interacting protein [Source:HGNC Symbol;Acc:HGNC:20343])

HSP 1 Score: 60.4622 bits (145), Expect = 2.314e-11
Identity = 30/98 (30.61%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+  + A   A+INV T E   +C+++T  G ++V Y +D+   ++     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVNNMFVSKSLRCADDVAYINVETKERNRYCLELTEAGLKVVGYAFDQ--VDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Human
Match: GSKIP (GSK3B interacting protein [Source:HGNC Symbol;Acc:HGNC:20343])

HSP 1 Score: 60.4622 bits (145), Expect = 2.314e-11
Identity = 30/98 (30.61%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+  + A   A+INV T E   +C+++T  G ++V Y +D+   ++     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVNNMFVSKSLRCADDVAYINVETKERNRYCLELTEAGLKVVGYAFDQ--VDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Human
Match: GSKIP (GSK3B interacting protein [Source:HGNC Symbol;Acc:HGNC:20343])

HSP 1 Score: 60.4622 bits (145), Expect = 2.314e-11
Identity = 30/98 (30.61%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+  + A   A+INV T E   +C+++T  G ++V Y +D+   ++     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVNNMFVSKSLRCADDVAYINVETKERNRYCLELTEAGLKVVGYAFDQ--VDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Celegans
Match: Y43F8B.2 (pep chromosome:WBcel235:V:19519925:19521981:-1 gene:WBGene00012813.1 transcript:Y43F8B.2e.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43F8B.2)

HSP 1 Score: 73.559 bits (179), Expect = 2.189e-16
Identity = 39/101 (38.61%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESE---QYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EAI A+ E+ F V+ + +SE          +NVTTLE   +C+++T +G+RI S + D    +    E   +YY++++ LM   S  YRERFS++LV+RLK
Sbjct:   29 EAIAAVHELSFAVQSISVSEMLPRTPDLIFVNVTTLEAQPYCLELTLKGWRITSLRSDCMVGDFTRLELFTKYYDSLYLLMDDISPGYRERFSEKLVQRLK 129
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Celegans
Match: Y43F8B.2 (pep chromosome:WBcel235:V:19519925:19522817:-1 gene:WBGene00012813.1 transcript:Y43F8B.2e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43F8B.2)

HSP 1 Score: 73.559 bits (179), Expect = 2.189e-16
Identity = 39/101 (38.61%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESE---QYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EAI A+ E+ F V+ + +SE          +NVTTLE   +C+++T +G+RI S + D    +    E   +YY++++ LM   S  YRERFS++LV+RLK
Sbjct:   29 EAIAAVHELSFAVQSISVSEMLPRTPDLIFVNVTTLEAQPYCLELTLKGWRITSLRSDCMVGDFTRLELFTKYYDSLYLLMDDISPGYRERFSEKLVQRLK 129
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Celegans
Match: Y43F8B.2 (pep chromosome:WBcel235:V:19519925:19522861:-1 gene:WBGene00012813.1 transcript:Y43F8B.2c.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43F8B.2)

HSP 1 Score: 73.9442 bits (180), Expect = 3.236e-16
Identity = 39/101 (38.61%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESE---QYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EAI A+ E+ F V+ + +SE          +NVTTLE   +C+++T +G+RI S + D    +    E   +YY++++ LM   S  YRERFS++LV+RLK
Sbjct:   52 EAIAAVHELSFAVQSISVSEMLPRTPDLIFVNVTTLEAQPYCLELTLKGWRITSLRSDCMVGDFTRLELFTKYYDSLYLLMDDISPGYRERFSEKLVQRLK 152
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Celegans
Match: Y43F8B.2 (pep chromosome:WBcel235:V:19519925:19523002:-1 gene:WBGene00012813.1 transcript:Y43F8B.2c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43F8B.2)

HSP 1 Score: 73.9442 bits (180), Expect = 3.236e-16
Identity = 39/101 (38.61%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESE---QYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EAI A+ E+ F V+ + +SE          +NVTTLE   +C+++T +G+RI S + D    +    E   +YY++++ LM   S  YRERFS++LV+RLK
Sbjct:   52 EAIAAVHELSFAVQSISVSEMLPRTPDLIFVNVTTLEAQPYCLELTLKGWRITSLRSDCMVGDFTRLELFTKYYDSLYLLMDDISPGYRERFSEKLVQRLK 152
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Celegans
Match: Y43F8B.2 (pep chromosome:WBcel235:V:19519925:19522861:-1 gene:WBGene00012813.1 transcript:Y43F8B.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43F8B.2)

HSP 1 Score: 73.9442 bits (180), Expect = 5.233e-16
Identity = 39/101 (38.61%), Postives = 61/101 (60.40%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESE---QYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EAI A+ E+ F V+ + +SE          +NVTTLE   +C+++T +G+RI S + D    +    E   +YY++++ LM   S  YRERFS++LV+RLK
Sbjct:   86 EAIAAVHELSFAVQSISVSEMLPRTPDLIFVNVTTLEAQPYCLELTLKGWRITSLRSDCMVGDFTRLELFTKYYDSLYLLMDDISPGYRERFSEKLVQRLK 186
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Fly
Match: CG14505 (gene:FBgn0034327 transcript:FBtr0110803)

HSP 1 Score: 61.2326 bits (147), Expect = 4.015e-12
Identity = 27/75 (36.00%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  176 VVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFS 400
            ++++E     +  A+IN+ TLE V +CVQ++  G+RIVSY++D+   E    +  YE+ H L++  S LY E++ 
Sbjct:   31 LIVAEFLPHCRGVAYINIRTLEQVIYCVQLSRAGYRIVSYEFDDVADEVANCDTVYESAHQLLAGISPLYGEKYG 105
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Fly
Match: CG14505 (gene:FBgn0034327 transcript:FBtr0307186)

HSP 1 Score: 61.2326 bits (147), Expect = 4.015e-12
Identity = 27/75 (36.00%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  176 VVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFS 400
            ++++E     +  A+IN+ TLE V +CVQ++  G+RIVSY++D+   E    +  YE+ H L++  S LY E++ 
Sbjct:   31 LIVAEFLPHCRGVAYINIRTLEQVIYCVQLSRAGYRIVSYEFDDVADEVANCDTVYESAHQLLAGISPLYGEKYG 105
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Fly
Match: CG11523 (gene:FBgn0037156 transcript:FBtr0078511)

HSP 1 Score: 48.9062 bits (115), Expect = 2.396e-7
Identity = 31/116 (26.72%), Postives = 59/116 (50.86%), Query Frame = 2 
Query:  122 CSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECE---------------SEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            C  EA   + ++K  V ++ +S K     +  ++N+ T+E+   CVQ+++ GF+IVS +YD +  E+                  E+ +ET + L+   S  Y E F ++L ++L+
Sbjct:   18 CEDEANAIINDVKAHVAEICISSKLASDATQIYLNIRTIESATCCVQVSSRGFKIVSSQYD-TIDEDSRISALLRNGQEQGDDEEEEIFETPYALLDKISPRYVESFGNQLCQQLR 132
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Zebrafish
Match: gskip (gsk3b interacting protein [Source:NCBI gene;Acc:406744])

HSP 1 Score: 60.077 bits (144), Expect = 1.898e-11
Identity = 31/99 (31.31%), Postives = 56/99 (56.57%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQ 427
            EA   + ++ F V  + +S         A+INV T E   +C+++T  G R+V + +D+    +  S QY+ET++ L+ + S  YRE F + L++RL++
Sbjct:   45 EAEAVVNDVLFAVSDMHVSHNLSSGLDVAYINVETREGNRYCLELTEAGLRVVGHTFDK--VNDGLSSQYHETVYSLLDSLSPGYREAFGNALLQRLER 141
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Xenopus
Match: gskip (GSK3B interacting protein [Source:NCBI gene;Acc:100125074])

HSP 1 Score: 62.003 bits (149), Expect = 4.371e-12
Identity = 32/99 (32.32%), Postives = 55/99 (55.56%), Query Frame = 2 
Query:  128 SEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            +EAI  + ++ F V  + +S+    A   A+INV   E   +C+++T  G R+  Y +D      C   QY+ET++ L+ + S  YRE F + L++RL+
Sbjct:   37 AEAI--VNDVLFAVGNMFVSKTLPCAVDVAYINVEIKEGTRYCLELTDAGLRVAGYAFDHLAEGLC--SQYHETVYSLLDSLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Mouse
Match: Gskip (GSK3B interacting protein [Source:MGI Symbol;Acc:MGI:1914037])

HSP 1 Score: 62.3882 bits (150), Expect = 3.144e-12
Identity = 34/105 (32.38%), Postives = 57/105 (54.29%), Query Frame = 2 
Query:  110 DEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            D K    EA   + ++ F V  + +S+    A   A+INV T E   +C+++T  G R+V Y +D+   E+     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   34 DMKDMQLEAEAVVNDVLFAVNHMFVSKSMPCADDVAYINVETKERNRYCLELTEAGLRVVGYAFDQ--VEDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 136
[Close]
BLAST of GSK3-beta interaction protein vs. UniProt/SwissProt
Match: sp|Q5ZMC6|GSKIP_CHICK (GSK3-beta interaction protein OS=Gallus gallus OX=9031 GN=GSKIP PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.197e-12
Identity = 32/98 (32.65%), Postives = 58/98 (59.18%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+    A+  A+INV T E   +CV++T  G R+V+Y +D+  +++     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVSNMFVSKTLPCAEDVAYINVETRERGRYCVELTDSGLRVVAYDFDQ--TDDSLQNPYHETVYSLLDTLSPAYREVFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. UniProt/SwissProt
Match: sp|Q5PPI3|GSKIP_RAT (GSK3B-interacting protein OS=Rattus norvegicus OX=10116 GN=Gskip PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.227e-11
Identity = 32/98 (32.65%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+    A   A+INV T E   +C+++T  G R+V Y +D+   E+     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVNNMFVSKSLPCADDVAYINVETKERNRYCLELTEAGLRVVGYAFDQ--VEDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. UniProt/SwissProt
Match: sp|Q8BGR8|GSKIP_MOUSE (GSK3B-interacting protein OS=Mus musculus OX=10090 GN=Gskip PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.767e-11
Identity = 34/105 (32.38%), Postives = 57/105 (54.29%), Query Frame = 2 
Query:  110 DEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            D K    EA   + ++ F V  + +S+    A   A+INV T E   +C+++T  G R+V Y +D+   E+     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   29 DMKDMQLEAEAVVNDVLFAVNHMFVSKSMPCADDVAYINVETKERNRYCLELTEAGLRVVGYAFDQ--VEDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. UniProt/SwissProt
Match: sp|A4IIW5|GSKIP_XENTR (GSK3-beta interaction protein OS=Xenopus tropicalis OX=8364 GN=gskip PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.316e-11
Identity = 32/99 (32.32%), Postives = 55/99 (55.56%), Query Frame = 2 
Query:  128 SEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            +EAI  + ++ F V  + +S+    A   A+INV   E   +C+++T  G R+  Y +D      C   QY+ET++ L+ + S  YRE F + L++RL+
Sbjct:   37 AEAI--VNDVLFAVGNMFVSKTLPCAVDVAYINVEIKEGTRYCLELTDAGLRVAGYAFDHLAEGLC--SQYHETVYSLLDSLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. UniProt/SwissProt
Match: sp|Q0P5A3|GSKIP_BOVIN (GSK3B-interacting protein OS=Bos taurus OX=9913 GN=GSKIP PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.474e-11
Identity = 31/98 (31.63%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V  + +S+  + A   A+INV T E   +C+++T  G R+V Y +D+   ++     Y+ET++ L+   S  YRE F + L++RL+
Sbjct:   36 EAEAVVNDVLFAVNTMFVSKTLRCADDVAYINVETRERNRYCLELTEAGLRVVGYAFDQ--VDDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLE 131
[Close]
BLAST of GSK3-beta interaction protein vs. TrEMBL
Match: A0A4S2KEY5 (DUF727 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011110 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 8.282e-20
Identity = 51/107 (47.66%), Postives = 70/107 (65.42%), Query Frame = 2 
Query:  104 DFDEKLCSSEAINALEEIKFGVKKVV-LSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRL 421
            D D KLCS EA  A++E+ FGVK +V  S+      S A++NVTTLE+   C++I+A GF  V  +YD+      ESE YYETI+ L+SA+S L+R++FS  L  RL
Sbjct:   19 DDDNKLCSVEAEAAIKEVAFGVKHIVRASDSLPYTDSLAYLNVTTLEDKKMCIEISARGFCPVGEEYDKVTDNAQESE-YYETIYALLSARSTLFRDQFSRRLCDRL 124
[Close]
BLAST of GSK3-beta interaction protein vs. TrEMBL
Match: G7YNI6 (GSK3-beta interaction protein OS=Clonorchis sinensis OX=79923 GN=CLF_103549 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.534e-19
Identity = 51/110 (46.36%), Postives = 73/110 (66.36%), Query Frame = 2 
Query:   98 SMDFDEKLCSSEAINALEEIKFGVKKVV-LSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
             +D D KLCS EA  A++E+ FGVK++V  S+      S A++NVTTLE+   C++I+A GF  V  +YD+      ESE YYETI+ L+SA+S L+R++FS  L  RL+
Sbjct:   91 PVDDDSKLCSVEAEAAIKEVAFGVKQIVRASDSLPYTDSLAYLNVTTLEDKKMCIEISARGFCPVGEEYDKVTDNTQESE-YYETIYALLSARSILFRDQFSRRLCDRLE 199
[Close]
BLAST of GSK3-beta interaction protein vs. TrEMBL
Match: A0A068YN22 (GSK3 beta interaction protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001126900 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.118e-18
Identity = 49/121 (40.50%), Postives = 72/121 (59.50%), Query Frame = 2 
Query:  110 DEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFS------EECESE-----QYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY 439
             +KLC+ EA  A+ E  FGV+ + ++E    ++S A+IN+TTLE    CV+I+A GFR V   YD+         +E E E     + YETI+ L+SA+S  +R RFS+ L +RL Q  D+
Sbjct:   28 GDKLCTLEAEAAVSETAFGVRDIRVAEALPFSESLAYINLTTLEGEKLCVEISAAGFRPVGVFYDDVVGPVPPPHQEGEEEPELGMENYETIYALLSARSSGFRHRFSERLAQRLAQLQDH 148
[Close]
BLAST of GSK3-beta interaction protein vs. TrEMBL
Match: A0A0R3TJX7 (DUF727 domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS7419 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.550e-18
Identity = 45/120 (37.50%), Postives = 70/120 (58.33%), Query Frame = 2 
Query:  113 EKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDE---------------SFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQ 427
            +KLC+ EA  A+ E  FGV+ + ++E    ++S A++N+TTLE    CV+I+A GFR V   YD+               + S +    ++YETI+ L+SA+S  +R+RFS  L +RL Q
Sbjct:   14 DKLCTLEAEAAVSETSFGVRDIRIAEALPFSESLAYLNLTTLEGERLCVEISALGFRPVGVDYDDVIGAVPPPKQDSSPSALSTDSPKFEHYETIYALLSARSAGFRDRFSQRLAERLAQ 133
[Close]
BLAST of GSK3-beta interaction protein vs. TrEMBL
Match: A0A5K3EPJ3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.884e-18
Identity = 45/117 (38.46%), Postives = 67/117 (57.26%), Query Frame = 2 
Query:  113 EKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQY------------YETIHCLMSAKSELYRERFSDELVKRLKQ 427
            EKLCS EA  A++E  FGV+ + ++E    + S A++N+TTLE    CV+I+ EGFR V   YD+          +            YETI+ L+SA+S  +R RFS++L +RL +
Sbjct:   16 EKLCSIEAKAAVKETAFGVRDIRIAEALPFSDSLAYLNLTTLEGERLCVEISVEGFRPVGIFYDDVIGPVPPPPSHSREDAKPAVAETYETIYALLSARSAGFRHRFSEKLAERLAE 132
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Cavefish
Match: gskip (gsk3b interacting protein [Source:NCBI gene;Acc:406744])

HSP 1 Score: 63.5438 bits (153), Expect = 7.110e-13
Identity = 33/99 (33.33%), Postives = 57/99 (57.58%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQ 427
            EA   + ++ F V  + +S+        A+INV T E   +C+++T  G R+V Y +D+   +E  S QY+ET++ L+ + S  YRE F + L+ RL++
Sbjct:   31 EAEAVVNDVLFAVTDMHVSQTLSNGLDVAYINVETREGNRYCLELTEAGLRVVGYSFDQ--VDEDLSNQYHETVYSLLDSLSPGYREAFGNALLHRLER 127
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Sea Lamprey
Match: gskip (gsk3b interacting protein [Source:NCBI gene;Acc:406744])

HSP 1 Score: 43.8986 bits (102), Expect = 2.414e-6
Identity = 28/99 (28.28%), Postives = 49/99 (49.49%), Query Frame = 2 
Query:  128 SEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            +EA   + ++ F V+ + LS     ++  A+INV T E   +CV+++  G + +      S     +    +ETI+ L+   S  YR+ FS  L  RL+
Sbjct:   35 TEADAIINDVVFAVRSMALSRTLPSSEYLAYINVETKEGQRYCVELSDMGLKAIEVCTGPSG----QHFPIHETIYSLLDTVSPAYRQAFSAALFHRLE 129
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Nematostella
Match: EDO33748 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRB6])

HSP 1 Score: 68.5514 bits (166), Expect = 2.715e-15
Identity = 36/103 (34.95%), Postives = 63/103 (61.17%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDE-SFSEE---CESEQYYETIHCLMSAKSELYRERFSDELVKRLKQ 427
            EA   +++I F V ++ +SEK   ++ +  +N+ T EN+ FCVQ+  +GFR+VS  +D  SF E+    ++  +YETI+ L+   S  Y + F +EL ++L +
Sbjct:   11 EAQGVIKDIAFSVSQISVSEKLPSSRESVFLNIETKENICFCVQLCIQGFRVVSNTFDFCSFDEKDSITQNTPFYETIYALLDTISPGYSQMFGNELSRKLSE 113
[Close]
BLAST of GSK3-beta interaction protein vs. Ensembl Medaka
Match: gskip (GSK3B interacting protein [Source:NCBI gene;Acc:101162797])

HSP 1 Score: 63.5438 bits (153), Expect = 1.637e-12
Identity = 34/98 (34.69%), Postives = 58/98 (59.18%), Query Frame = 2 
Query:  131 EAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLK 424
            EA   + ++ F V ++ +S+    A   A+INV T E   +C+++T  G R+V + +D+   +E  S QY+ETI+ L+   S  YRE F + L++RL+
Sbjct:   64 EAEAVVNDVLFAVSEMHVSQNLGSASDVAYINVETREGNRYCLELTEAGLRVVGHVFDQ--VDEDLSTQYHETIYSLLDTLSPGYREAFGNALLQRLE 159
[Close]
BLAST of GSK3-beta interaction protein vs. Planmine SMEST
Match: SMESG000044959.1 (SMESG000044959.1)

HSP 1 Score: 228.794 bits (582), Expect = 5.702e-78
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 2 
Query:  101 MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY 439
            MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY
Sbjct:    1 MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY 113
[Close]
BLAST of GSK3-beta interaction protein vs. Planmine SMEST
Match: SMESG000019468.1 (SMESG000019468.1)

HSP 1 Score: 228.794 bits (582), Expect = 5.702e-78
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 2 
Query:  101 MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY 439
            MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY
Sbjct:    1 MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVDFCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFSDELVKRLKQHIDY 113
The following BLAST results are available for this feature:
BLAST of GSK3-beta interaction protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
GSKIP2.314e-1130.61GSK3B interacting protein [Source:HGNC Symbol;Acc:... [more]
GSKIP2.314e-1130.61GSK3B interacting protein [Source:HGNC Symbol;Acc:... [more]
GSKIP2.314e-1130.61GSK3B interacting protein [Source:HGNC Symbol;Acc:... [more]
GSKIP2.314e-1130.61GSK3B interacting protein [Source:HGNC Symbol;Acc:... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Y43F8B.22.189e-1638.61pep chromosome:WBcel235:V:19519925:19521981:-1 gen... [more]
Y43F8B.22.189e-1638.61pep chromosome:WBcel235:V:19519925:19522817:-1 gen... [more]
Y43F8B.23.236e-1638.61pep chromosome:WBcel235:V:19519925:19522861:-1 gen... [more]
Y43F8B.23.236e-1638.61pep chromosome:WBcel235:V:19519925:19523002:-1 gen... [more]
Y43F8B.25.233e-1638.61pep chromosome:WBcel235:V:19519925:19522861:-1 gen... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CG145054.015e-1236.00gene:FBgn0034327 transcript:FBtr0110803[more]
CG145054.015e-1236.00gene:FBgn0034327 transcript:FBtr0307186[more]
CG115232.396e-726.72gene:FBgn0037156 transcript:FBtr0078511[more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gskip1.898e-1131.31gsk3b interacting protein [Source:NCBI gene;Acc:40... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gskip4.371e-1232.32GSK3B interacting protein [Source:NCBI gene;Acc:10... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gskip3.144e-1232.38GSK3B interacting protein [Source:MGI Symbol;Acc:M... [more]
back to top
BLAST of GSK3-beta interaction protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5ZMC6|GSKIP_CHICK1.197e-1232.65GSK3-beta interaction protein OS=Gallus gallus OX=... [more]
sp|Q5PPI3|GSKIP_RAT1.227e-1132.65GSK3B-interacting protein OS=Rattus norvegicus OX=... [more]
sp|Q8BGR8|GSKIP_MOUSE1.767e-1132.38GSK3B-interacting protein OS=Mus musculus OX=10090... [more]
sp|A4IIW5|GSKIP_XENTR2.316e-1132.32GSK3-beta interaction protein OS=Xenopus tropicali... [more]
sp|Q0P5A3|GSKIP_BOVIN3.474e-1131.63GSK3B-interacting protein OS=Bos taurus OX=9913 GN... [more]
back to top
BLAST of GSK3-beta interaction protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2KEY58.282e-2047.66DUF727 domain-containing protein OS=Opisthorchis f... [more]
G7YNI65.534e-1946.36GSK3-beta interaction protein OS=Clonorchis sinens... [more]
A0A068YN222.118e-1840.50GSK3 beta interaction protein OS=Echinococcus mult... [more]
A0A0R3TJX72.550e-1837.50DUF727 domain-containing protein OS=Rodentolepis n... [more]
A0A5K3EPJ34.884e-1838.46Uncharacterized protein OS=Mesocestoides corti OX=... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gskip7.110e-1333.33gsk3b interacting protein [Source:NCBI gene;Acc:40... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gskip2.414e-628.28gsk3b interacting protein [Source:NCBI gene;Acc:40... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO337482.715e-1534.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gskip1.637e-1234.69GSK3B interacting protein [Source:NCBI gene;Acc:10... [more]
back to top
BLAST of GSK3-beta interaction protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000044959.15.702e-78100.00SMESG000044959.1[more]
SMESG000019468.15.702e-78100.00SMESG000019468.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030458 ID=SMED30030458|Name=GSK3-beta interaction protein|organism=Schmidtea mediterranea sexual|type=transcript|length=588bp
CCTTGTTTAAATATAGAATAGGTAAATTTGGACATTTTAAAAATTGAAAA
TTTTATTGTCTTTTAATTTTTGGATTTTTAGAAATTTTATTATTTAATCA
ATGGATTTTGATGAAAAATTATGTTCCTCTGAAGCAATTAATGCTTTAGA
AGAAATAAAATTTGGTGTGAAAAAAGTTGTACTAAGTGAAAAATTCCAAA
TTGCAAAAAGTACTGCACATATCAATGTAACCACTTTAGAAAATGTTGAT
TTCTGTGTACAGATAACTGCTGAAGGATTTCGTATTGTTTCCTATAAATA
CGATGAGTCGTTTTCTGAAGAGTGTGAATCTGAACAATATTATGAAACAA
TTCATTGTTTAATGAGCGCCAAAAGTGAATTATATAGAGAAAGATTTTCG
GATGAACTAGTTAAAAGACTGAAACAACACATAGATTATTAGAAACATAT
TTTTGTTAACTGCTTCTAATAATAATGATATATAAATGTTAAGATTTGTT
CTCGATATTTATATTTTAATAAATTGCGTAAACTTTTTAAAACATATTTT
GAGCCCTTTAAAATGGAGCTTATGGTTGTAATTAAATG
back to top

protein sequence of SMED30030458-orf-1

>SMED30030458-orf-1 ID=SMED30030458-orf-1|Name=SMED30030458-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp
MDFDEKLCSSEAINALEEIKFGVKKVVLSEKFQIAKSTAHINVTTLENVD
FCVQITAEGFRIVSYKYDESFSEECESEQYYETIHCLMSAKSELYRERFS
DELVKRLKQHIDY*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0060828regulation of canonical Wnt signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR037395GSKIP
IPR007967DUF727
IPR023231GSKIP_dom_sf
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007967GSKIP domainPFAMPF05303DUF727coord: 8..109
e-value: 3.6E-30
score: 104.1
IPR023231GSKIP domain superfamilyGENE3DG3DSA:3.30.2280.10coord: 8..112
e-value: 3.3E-30
score: 106.1
NoneNo IPR availablePANTHERPTHR12490:SF4GSK3B-INTERACTING PROTEINcoord: 4..109
NoneNo IPR availablePRODOMPD080843coord: 11..99
e-value: 2.0E-11
score: 160.0
NoneNo IPR availableSUPERFAMILYSSF103107Hypothetical protein c14orf129, hspc210coord: 7..111
IPR037395GSK3B-interacting proteinPANTHERPTHR12490UNCHARACTERIZEDcoord: 4..109