MACPF domain-containing protein

Overview
NameMACPF domain-containing protein
Smed IDSMED30030421
Length (bp)802
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MACPF domain-containing protein (SMED30030421) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MACPF domain-containing protein (SMED30030421) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030421

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30030421h1SMcG0006951 SmedASXL_010331SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30030421h1SMcG0006951 SmedASXL_010330SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30030421h1SMcG0006951 Contig31625newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030421h1SMcG0006951 Contig31625uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30030421h1SMcG0006951 dd_Smed_v4_12605_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030421h1SMcG0006950 Contig31625newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030421h1SMcG0006950 Contig31625uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
reproductive organSMED30030421h1SMcG0006951 Contig48191newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30030421h1SMcG0006951 Contig48191uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of MACPF domain-containing protein vs. Ensembl Human
Match: MPEG1 (macrophage expressed 1 [Source:HGNC Symbol;Acc:HGNC:29619])

HSP 1 Score: 92.4337 bits (228), Expect = 1.311e-20
Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 3
Query:  195 KLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            ++  Q C+        +VLPG GWDNL N+DMGRV++L +S C  + D +Y+IP+E+   P+K S +E++SEI   + NY SSTS SIN   E S+   K+ G FS E  ++K  Q
Sbjct:   28 EVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140          
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.1 (macrophage expressed 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-7347])

HSP 1 Score: 91.6633 bits (226), Expect = 1.847e-20
Identity = 50/111 (45.05%), Postives = 70/111 (63.06%), Query Frame = 3
Query:  213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVE-GSVEDLKLGGAFSFENTQIKNDQ 542
            C+ +++    +VLPG GWDNL NIDMGRV++L++S C  + D  YLIP+E+ + P+K S VE +SEI   ++   SSTS S+N  V   SV    L   FS EN ++K  Q
Sbjct:   33 CRKNSSLTALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKVSGVETNSEIIMSWLEQKSSTSSSVNADVSFFSV----LNAKFSTENQRMKTHQ 139          
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.1 (macrophage expressed 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-7347])

HSP 1 Score: 91.6633 bits (226), Expect = 1.847e-20
Identity = 50/111 (45.05%), Postives = 70/111 (63.06%), Query Frame = 3
Query:  213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVE-GSVEDLKLGGAFSFENTQIKNDQ 542
            C+ +++    +VLPG GWDNL NIDMGRV++L++S C  + D  YLIP+E+ + P+K S VE +SEI   ++   SSTS S+N  V   SV    L   FS EN ++K  Q
Sbjct:   33 CRKNSSLTALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKVSGVETNSEIIMSWLEQKSSTSSSVNADVSFFSV----LNAKFSTENQRMKTHQ 139          
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.2 (macrophage expressed 1, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-050522-305])

HSP 1 Score: 89.3521 bits (220), Expect = 1.152e-19
Identity = 49/112 (43.75%), Postives = 70/112 (62.50%), Query Frame = 3
Query:  207 QNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            + C+ ++     +VLPG GWDNL N+D+GRV++L +S C  + D  YLIP+E+ + P KTS VE  SE    +++  SSTS SIN   + S   + L G FS ENT++K  Q
Sbjct:   28 RECRQNSNLTALEVLPGGGWDNLRNLDVGRVMNLTYSQCQTTEDGLYLIPDEVFVIPRKTSGVETHSETIMSWLDQRSSTSGSIN--ADASYLTV-LNGKFSEENTRMKIHQ 136          
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.3 (macrophage expressed 1, tandem duplicate 3 [Source:ZFIN;Acc:ZDB-GENE-081105-5])

HSP 1 Score: 88.1965 bits (217), Expect = 2.259e-19
Identity = 48/110 (43.64%), Postives = 68/110 (61.82%), Query Frame = 3
Query:  213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            C  ++     +VLPG GWDNL NIDMGRV++L++S C  + D  YLIP+E+ + P+K + VE +SE+   +++  SSTS SIN  V        L G FS E+ +IK  Q
Sbjct:   33 CHKNSNLVALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKENTVETNSEMIMSWLDQKSSTSSSINADVSFFSW---LNGKFSREHQRIKTHQ 139          
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.1 (macrophage expressed 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-7347])

HSP 1 Score: 68.1662 bits (165), Expect = 1.941e-14
Identity = 28/59 (47.46%), Postives = 43/59 (72.88%), Query Frame = 3
Query:  213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTS 389
            C+ +++    +VLPG GWDNL NIDMGRV++L++S C  + D  YLIP+E+ + P+K +
Sbjct:   33 CRKNSSLTALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKKT 91          
BLAST of MACPF domain-containing protein vs. Ensembl Mouse
Match: Pfpl (pore forming protein-like [Source:MGI Symbol;Acc:MGI:1860266])

HSP 1 Score: 91.2781 bits (225), Expect = 2.552e-20
Identity = 47/113 (41.59%), Postives = 71/113 (62.83%), Query Frame = 3
Query:  204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +Q CQ        +VLPG GWDNL N+DMG+V+DL ++ C  + D +Y+IP+++   P+K S +E++SEI   + NY S+TS SINL ++      ++ G FS E  +IK  Q
Sbjct:   31 FQKCQNALNLPVLEVLPGGGWDNLRNMDMGQVMDLTYNNCKTTEDGQYIIPDDVFTIPQKESNLEMNSEILDSWENYRSTTSFSINLNLD---YRPRVNGKFSSEFQRIKTTQ 140          
BLAST of MACPF domain-containing protein vs. Ensembl Mouse
Match: Mpeg1 (macrophage expressed gene 1 [Source:MGI Symbol;Acc:MGI:1333743])

HSP 1 Score: 90.1225 bits (222), Expect = 6.504e-20
Identity = 48/115 (41.74%), Postives = 71/115 (61.74%), Query Frame = 3
Query:  204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSIN--LAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +Q C+        +VLPG GWDNL N+DMGRV+DL ++ C  + D +Y+IP+E+   P+K S +E++SE+   ++NY S+TS SIN  LA+   V      G FS E  ++K  Q
Sbjct:   38 FQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVN-----GKFSTEFQRMKTLQ 147          
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2KJC3|MPEG1_BOVIN (Macrophage-expressed gene 1 protein OS=Bos taurus OX=9913 GN=MPEG1 PE=2 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 8.074e-21
Identity = 50/113 (44.25%), Postives = 72/113 (63.72%), Query Frame = 3
Query:  204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +QNC+         VLPG GWDNL N+D GRV++L +S C  + D +Y++P+E+   P+K S +E++S+I   +VNY SSTS SIN+  E S+   K+ G FS E  +IK  Q
Sbjct:   31 FQNCKNALKLPVLPVLPGGGWDNLRNVDTGRVMELAYSHCRTTEDGQYIVPDEIFTIPQKQSNLEMNSKILESWVNYQSSTSNSINM--ELSLFS-KVNGKFSLEFQRIKTLQ 140          
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2M385|MPEG1_HUMAN (Macrophage-expressed gene 1 protein OS=Homo sapiens OX=9606 GN=MPEG1 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.295e-20
Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 3
Query:  195 KLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            ++  Q C+        +VLPG GWDNL N+DMGRV++L +S C  + D +Y+IP+E+   P+K S +E++SEI   + NY SSTS SIN   E S+   K+ G FS E  ++K  Q
Sbjct:   28 EVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140          
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5RBP9|MPEG1_PONAB (Macrophage-expressed gene 1 protein OS=Pongo abelii OX=9601 GN=MPEG1 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.355e-20
Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 3
Query:  195 KLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            ++  Q C+        +VLPG GWDNL N+DMGRV++L +S C  + D +Y+IP+E+   P+K S +E++SEI   + NY SSTS SIN   E S+   K+ G FS E  ++K  Q
Sbjct:   28 EVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140          
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9WV57|MPEG1_RAT (Macrophage-expressed gene 1 protein OS=Rattus norvegicus OX=10116 GN=Mpeg1 PE=2 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 6.531e-20
Identity = 51/113 (45.13%), Postives = 72/113 (63.72%), Query Frame = 3
Query:  204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +Q C+        +VLPG GWDNL NIDMGRV+DL ++ C  + D +Y+IP+E+   P+K S +E++SEI   +VNY S+TS SIN   E S+   K+ G FS E  ++K  Q
Sbjct:   31 FQTCKDTLKLPVLEVLPGGGWDNLRNIDMGRVIDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEIRDSWVNYQSTTSFSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140          
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|A1L314|MPEG1_MOUSE (Macrophage-expressed gene 1 protein OS=Mus musculus OX=10090 GN=Mpeg1 PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 4.533e-19
Identity = 48/115 (41.74%), Postives = 71/115 (61.74%), Query Frame = 3
Query:  204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSIN--LAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +Q C+        +VLPG GWDNL N+DMGRV+DL ++ C  + D +Y+IP+E+   P+K S +E++SE+   ++NY S+TS SIN  LA+   V      G FS E  ++K  Q
Sbjct:   31 FQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVN-----GKFSTEFQRMKTLQ 140          
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A267EFV7 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003757g1 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.573e-22
Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3
Query:  240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533
            F  +PG GWDNL N DMG V DL+F+ C  S+D  Y++P  + LYP K S +E+ SE+  HF NYTSS S SINL   G +   ++GG  S E  Q+K
Sbjct:   51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148          
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A267FTE5 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003757g3 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 6.241e-22
Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3
Query:  240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533
            F  +PG GWDNL N DMG V DL+F+ C  S+D  Y++P  + LYP K S +E+ SE+  HF NYTSS S SINL   G +   ++GG  S E  Q+K
Sbjct:   51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148          
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A1I8IZJ2 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.039e-21
Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3
Query:  240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533
            F  +PG GWDNL N DMG V DL+F+ C  S+D  Y++P  + LYP K S +E+ SE+  HF NYTSS S SINL   G +   ++GG  S E  Q+K
Sbjct:   51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148          
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A1I8FUV4 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.142e-21
Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3
Query:  240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533
            F  +PG GWDNL N DMG V DL+F+ C  S+D  Y++P  + LYP K S +E+ SE+  HF NYTSS S SINL   G +   ++GG  S E  Q+K
Sbjct:   51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148          
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A2B4RFA8 (Macrophage-expressed gene 1 protein OS=Stylophora pistillata OX=50429 GN=Mpeg1 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.397e-21
Identity = 49/112 (43.75%), Postives = 73/112 (65.18%), Query Frame = 3
Query:  207 QNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +NC L +    F+VLPG GWDNL N+ MG V  +N+S C  S+D KYLIP+++ LYP K S+V + +E++ H+ NY+S+T+KSIN    G+     + G++S E   +K  Q
Sbjct:   44 RNCPLASGVYRFEVLPGGGWDNLRNLHMGAVSVMNYSKCKTSDDGKYLIPDDVFLYPVKESKVHVFAEMYDHWNNYSSTTTKSINAEASGTFGFGSVSGSYSSEFESVKKHQ 155          
BLAST of MACPF domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000015822.2 (pep primary_assembly:Astyanax_mexicanus-2.0:6:8259512:8262317:1 gene:ENSAMXG00000015374.2 transcript:ENSAMXT00000015822.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.855 bits (159), Expect = 7.305e-12
Identity = 37/85 (43.53%), Postives = 56/85 (65.88%), Query Frame = 3
Query:  288 MGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            MGRV+++++S C  + D  YLIP+E+ + P+K S+VE++SEI S ++   SSTS SIN   +GS   + L   FS EN ++K  Q
Sbjct:    1 MGRVMNMSYSQCQTTEDGVYLIPDEVFVIPQKVSKVEMNSEIISSWLEQRSSTSGSIN--ADGSFFSV-LNCKFSAENQRMKTHQ 82          
BLAST of MACPF domain-containing protein vs. Ensembl Cavefish
Match: MPEG1 (macrophage expressed 1 [Source:HGNC Symbol;Acc:HGNC:29619])

HSP 1 Score: 65.0846 bits (157), Expect = 1.334e-11
Identity = 38/86 (44.19%), Postives = 56/86 (65.12%), Query Frame = 3
Query:  288 MGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVE-GSVEDLKLGGAFSFENTQIKNDQ 542
            MGRV+++++S C  + D  YLIP+E+ + P+K S+VE++SEI S ++   SSTS SIN  V   SV + K    FS EN ++K  Q
Sbjct:    1 MGRVMNISYSQCQTTEDGVYLIPDEVFVIPQKVSKVEMNSEIISSWLEQKSSTSSSINADVSFFSVVNAK----FSTENQRMKTHQ 82          
BLAST of MACPF domain-containing protein vs. Ensembl Nematostella
Match: EDO28266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6Y8])

HSP 1 Score: 87.4261 bits (215), Expect = 5.302e-22
Identity = 39/78 (50.00%), Postives = 55/78 (70.51%), Query Frame = 3
Query:  243 DVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEG 476
            +VLPG GWDNL N+ MG V  +N+SLC  S+D K+LIP+ + LYP K S+V   +E + H+ NY+S+T+KSIN   + 
Sbjct:    1 EVLPGGGWDNLRNLHMGAVSAMNYSLCRSSDDGKFLIPDNVFLYPVKKSKVNTFAEFYDHWNNYSSTTTKSINAEAKA 78          
BLAST of MACPF domain-containing protein vs. Ensembl Nematostella
Match: EDO37879 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEK6])

HSP 1 Score: 86.6557 bits (213), Expect = 2.372e-19
Identity = 45/100 (45.00%), Postives = 65/100 (65.00%), Query Frame = 3
Query:  243 DVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +VLPG GWDNL N+ MG V  +N+SLC  S+D K+L+P+ + LYP K S+V   +E + H+ NY+S+T+KSIN   + S     + G+FS E   +K  Q
Sbjct:    1 EVLPGGGWDNLRNLHMGAVSAMNYSLCRSSDDGKFLVPDNVFLYPVKKSKVNTFAEFYDHWNNYSSTTTKSINAEAKASFGFGSVSGSFSSEYESVKKHQ 100          
BLAST of MACPF domain-containing protein vs. Ensembl Medaka
Match: MPEG1 (macrophage-expressed gene 1 protein [Source:NCBI gene;Acc:101169041])

HSP 1 Score: 78.9518 bits (193), Expect = 2.784e-16
Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = 3
Query:  237 TFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLK----LGGAFSFENTQIKNDQ 542
              +VLPG GWDNL N+DMGRV++ ++  C  + D  YLIP+E+ + P K + VE +SEI S +++   ST+ SIN       +D+     L   FS E T++K  Q
Sbjct:   69 ALEVLPGGGWDNLRNMDMGRVMNFSYFQCQTTEDGLYLIPDEVFVVPYKETGVETNSEIISSWLDQKGSTAASIN-------DDVSFLPVLNAKFSVEITRMKTHQ 167          
BLAST of MACPF domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000006104.2 (pep primary_assembly:ASM223467v1:9:9652430:9669002:1 gene:ENSORLG00000027013.1 transcript:ENSORLT00000006104.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 78.1814 bits (191), Expect = 5.881e-16
Identity = 46/115 (40.00%), Postives = 66/115 (57.39%), Query Frame = 3
Query:  207 QNCQLDT--TQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLK-LGGAFSFENTQIKNDQ 542
            + C+  T  + K  +VLPG GWDNL N+DMGRV++  +  C  + D  YL P+E+ + P K + VE +SEI S +++   ST+ SIN      V DL  L   FS E  ++K  Q
Sbjct:   29 RQCRASTNLSLKALEVLPGGGWDNLRNMDMGRVMNFGYFQCQTTKDGLYLNPDEVFVVPYKETGVETNSEIISSWLDQKGSTAASIN----DDVSDLPVLNAKFSSEIIRMKTHQ 139          
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044680.1 (SMESG000044680.1)

HSP 1 Score: 298.516 bits (763), Expect = 1.168e-103
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 3
Query:  108 MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ
Sbjct:    1 MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 145          
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044677.1 (SMESG000044677.1)

HSP 1 Score: 182.185 bits (461), Expect = 4.507e-54
Identity = 90/146 (61.64%), Postives = 111/146 (76.03%), Query Frame = 3
Query:  105 IMTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            I  +L + LF S        +  + EV  +K +YQ C+L + QKTFDVLPG+GWDNLANIDMGR+LDLNFS C FSNDNKYLIPN+LA+YPEK+S+VEISSEIF HF NYTS +S+SIN+  EG     K+GG+FS ENT+IK+DQ
Sbjct:    3 IQIVLIISLFRS-------AVKGSNEVNTIKDSYQLCRLTSKQKTFDVLPGSGWDNLANIDMGRILDLNFSKCSFSNDNKYLIPNQLAIYPEKSSQVEISSEIFHHFSNYTSMSSQSINVGAEGGYGIFKIGGSFSTENTKIKSDQ 141          
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044677.1 (SMESG000044677.1)

HSP 1 Score: 182.57 bits (462), Expect = 8.182e-54
Identity = 90/147 (61.22%), Postives = 111/147 (75.51%), Query Frame = 3
Query:  105 IMTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQG 545
            I  +L + LF S        +  + EV  +K +YQ C+L + QKTFDVLPG+GWDNLANIDMGR+LDLNFS C FSNDNKYLIPN+LA+YPEK+S+VEISSEIF HF NYTS +S+SIN+  EG     K+GG+FS ENT+IK+DQ 
Sbjct:    3 IQIVLIISLFRS-------AVKGSNEVNTIKDSYQLCRLTSKQKTFDVLPGSGWDNLANIDMGRILDLNFSKCSFSNDNKYLIPNQLAIYPEKSSQVEISSEIFHHFSNYTSMSSQSINVGAEGGYGIFKIGGSFSTENTKIKSDQA 142          
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044681.1 (SMESG000044681.1)

HSP 1 Score: 177.563 bits (449), Expect = 3.680e-51
Identity = 84/126 (66.67%), Postives = 101/126 (80.16%), Query Frame = 3
Query:  165 ILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            +  + EV  +K  YQ CQL   Q+TFDVLPG+GWDNLANI MGR+LDLNFS C FSNDNKYLIPN+LA+YPEK+S+VEISSEIF HF NYTS +S+SIN+  EG     K+GG+FS ENT+IK+DQ
Sbjct:   16 VKGSNEVNTIKDAYQLCQLMPNQRTFDVLPGSGWDNLANIGMGRILDLNFSKCSFSNDNKYLIPNQLAIYPEKSSQVEISSEIFRHFSNYTSMSSQSINVGAEGGYGIFKIGGSFSTENTKIKSDQ 141          
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044682.1 (SMESG000044682.1)

HSP 1 Score: 168.318 bits (425), Expect = 1.314e-47
Identity = 79/113 (69.91%), Postives = 94/113 (83.19%), Query Frame = 3
Query:  204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542
            Y+ C L + Q TFDVLPG+GWDNLAN+ MGR+LDLNFS C FSNDNKYLIPN+LA+YP+KTS+VEISSEIF HF NYTS +S+SINL  EG     K+GG+FS ENT+IK+DQ
Sbjct:   32 YELCHLRSNQLTFDVLPGSGWDNLANVGMGRILDLNFSKCSFSNDNKYLIPNQLAIYPQKTSQVEISSEIFRHFSNYTSMSSQSINLDAEGLFGHWKIGGSFSTENTKIKSDQ 144          
The following BLAST results are available for this feature:
BLAST of MACPF domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MPEG11.311e-2043.10macrophage expressed 1 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of MACPF domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MACPF domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mpeg1.11.847e-2045.05macrophage expressed 1, tandem duplicate 1 [Source... [more]
mpeg1.11.847e-2045.05macrophage expressed 1, tandem duplicate 1 [Source... [more]
mpeg1.21.152e-1943.75macrophage expressed 1, tandem duplicate 2 [Source... [more]
mpeg1.32.259e-1943.64macrophage expressed 1, tandem duplicate 3 [Source... [more]
mpeg1.11.941e-1447.46macrophage expressed 1, tandem duplicate 1 [Source... [more]
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BLAST of MACPF domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MACPF domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Pfpl2.552e-2041.59pore forming protein-like [Source:MGI Symbol;Acc:M... [more]
Mpeg16.504e-2041.74macrophage expressed gene 1 [Source:MGI Symbol;Acc... [more]
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BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q2KJC3|MPEG1_BOVIN8.074e-2144.25Macrophage-expressed gene 1 protein OS=Bos taurus ... [more]
sp|Q2M385|MPEG1_HUMAN6.295e-2043.10Macrophage-expressed gene 1 protein OS=Homo sapien... [more]
sp|Q5RBP9|MPEG1_PONAB6.355e-2043.10Macrophage-expressed gene 1 protein OS=Pongo abeli... [more]
sp|Q9WV57|MPEG1_RAT6.531e-2045.13Macrophage-expressed gene 1 protein OS=Rattus norv... [more]
sp|A1L314|MPEG1_MOUSE4.533e-1941.74Macrophage-expressed gene 1 protein OS=Mus musculu... [more]
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BLAST of MACPF domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267EFV73.573e-2248.98MACPF domain-containing protein OS=Macrostomum lig... [more]
A0A267FTE56.241e-2248.98MACPF domain-containing protein OS=Macrostomum lig... [more]
A0A1I8IZJ21.039e-2148.98MACPF domain-containing protein OS=Macrostomum lig... [more]
A0A1I8FUV41.142e-2148.98MACPF domain-containing protein OS=Macrostomum lig... [more]
A0A2B4RFA82.397e-2143.75Macrophage-expressed gene 1 protein OS=Stylophora ... [more]
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BLAST of MACPF domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000015822.27.305e-1243.53pep primary_assembly:Astyanax_mexicanus-2.0:6:8259... [more]
MPEG11.334e-1144.19macrophage expressed 1 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of MACPF domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MACPF domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MACPF domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO282665.302e-2250.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO378792.372e-1945.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of MACPF domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MPEG12.784e-1640.57macrophage-expressed gene 1 protein [Source:NCBI g... [more]
ENSORLT00000006104.25.881e-1640.00pep primary_assembly:ASM223467v1:9:9652430:9669002... [more]
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BLAST of MACPF domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000044680.11.168e-103100.00SMESG000044680.1[more]
SMESG000044677.14.507e-5461.64SMESG000044677.1[more]
SMESG000044677.18.182e-5461.22SMESG000044677.1[more]
SMESG000044681.13.680e-5166.67SMESG000044681.1[more]
SMESG000044682.11.314e-4769.91SMESG000044682.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030421 ID=SMED30030421|Name=MACPF domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=802bp
ATTGCATGAAGATATTTCAAATTGTTATTTAATGTGCACCCCATGACGCA
AATTATAATTGTATAAAATAATTATCTCATTCAAGAAGTTTCATTTCAGG
AGCAATAATGACCATTTTATGGGTGTTTTTATTTGCATCAATTCATGGAC
GTTTTGTATATGGAATATTAAATACAAAGGAAGTCACGAATTTAAAACTT
AATTACCAGAATTGTCAGTTAGATACGACCCAAAAGACGTTTGATGTGCT
ACCAGGAACAGGATGGGATAACTTGGCCAACATTGATATGGGAAGAGTTT
TGGACCTTAACTTCTCCTTATGCTTATTCTCCAATGATAATAAATATCTG
ATTCCCAATGAATTGGCTCTATATCCGGAAAAAACTTCAAGAGTAGAAAT
TTCTAGTGAAATCTTTAGTCATTTTGTGAATTATACATCTTCAACGTCAA
AATCCATCAACTTGGCCGTAGAAGGAAGTGTTGAGGATTTGAAATTAGGT
GGAGCGTTTTCATTTGAAAATACACAAATAAAGAATGATCAAGGTTGATC
AACGTTTGAGTGACAACCTCTCAATGTATTTACCCAATTCTTCGCAGTTG
ATAACAAGTGGGCCACCCTCTGACCGGGAGACTACAACAGCTCGTGAAGT
TCACTGCTAGTTTTCATTTGACAAAAAGAAATTTTATTCCGCTTCGATAT
TTAATAATTGAAATCATGATATTTTCCATTTTTCAACGCTCTGGCGCTAT
TTCAATCAAGCAAAATGACATATTGGACACAAAATCCATTTTCACCAGAG
TT
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protein sequence of SMED30030421-orf-1

>SMED30030421-orf-1 ID=SMED30030421-orf-1|Name=SMED30030421-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=147bp
MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPG
TGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISS
EIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR039707MPEG1
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039707Macrophage-expressed gene 1 proteinPANTHERPTHR31463FAMILY NOT NAMEDcoord: 29..145
NoneNo IPR availablePANTHERPTHR31463:SF4MACROPHAGE-EXPRESSED GENE 1 PROTEINcoord: 29..145
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.704