MACPF domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030421 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of MACPF domain-containing protein vs. Ensembl Human
Match: MPEG1 (macrophage expressed 1 [Source:HGNC Symbol;Acc:HGNC:29619]) HSP 1 Score: 92.4337 bits (228), Expect = 1.311e-20 Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 3 Query: 195 KLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 ++ Q C+ +VLPG GWDNL N+DMGRV++L +S C + D +Y+IP+E+ P+K S +E++SEI + NY SSTS SIN E S+ K+ G FS E ++K Q Sbjct: 28 EVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.1 (macrophage expressed 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-7347]) HSP 1 Score: 91.6633 bits (226), Expect = 1.847e-20 Identity = 50/111 (45.05%), Postives = 70/111 (63.06%), Query Frame = 3 Query: 213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVE-GSVEDLKLGGAFSFENTQIKNDQ 542 C+ +++ +VLPG GWDNL NIDMGRV++L++S C + D YLIP+E+ + P+K S VE +SEI ++ SSTS S+N V SV L FS EN ++K Q Sbjct: 33 CRKNSSLTALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKVSGVETNSEIIMSWLEQKSSTSSSVNADVSFFSV----LNAKFSTENQRMKTHQ 139
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.1 (macrophage expressed 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-7347]) HSP 1 Score: 91.6633 bits (226), Expect = 1.847e-20 Identity = 50/111 (45.05%), Postives = 70/111 (63.06%), Query Frame = 3 Query: 213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVE-GSVEDLKLGGAFSFENTQIKNDQ 542 C+ +++ +VLPG GWDNL NIDMGRV++L++S C + D YLIP+E+ + P+K S VE +SEI ++ SSTS S+N V SV L FS EN ++K Q Sbjct: 33 CRKNSSLTALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKVSGVETNSEIIMSWLEQKSSTSSSVNADVSFFSV----LNAKFSTENQRMKTHQ 139
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.2 (macrophage expressed 1, tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-050522-305]) HSP 1 Score: 89.3521 bits (220), Expect = 1.152e-19 Identity = 49/112 (43.75%), Postives = 70/112 (62.50%), Query Frame = 3 Query: 207 QNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 + C+ ++ +VLPG GWDNL N+D+GRV++L +S C + D YLIP+E+ + P KTS VE SE +++ SSTS SIN + S + L G FS ENT++K Q Sbjct: 28 RECRQNSNLTALEVLPGGGWDNLRNLDVGRVMNLTYSQCQTTEDGLYLIPDEVFVIPRKTSGVETHSETIMSWLDQRSSTSGSIN--ADASYLTV-LNGKFSEENTRMKIHQ 136
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.3 (macrophage expressed 1, tandem duplicate 3 [Source:ZFIN;Acc:ZDB-GENE-081105-5]) HSP 1 Score: 88.1965 bits (217), Expect = 2.259e-19 Identity = 48/110 (43.64%), Postives = 68/110 (61.82%), Query Frame = 3 Query: 213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 C ++ +VLPG GWDNL NIDMGRV++L++S C + D YLIP+E+ + P+K + VE +SE+ +++ SSTS SIN V L G FS E+ +IK Q Sbjct: 33 CHKNSNLVALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKENTVETNSEMIMSWLDQKSSTSSSINADVSFFSW---LNGKFSREHQRIKTHQ 139
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Match: mpeg1.1 (macrophage expressed 1, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030131-7347]) HSP 1 Score: 68.1662 bits (165), Expect = 1.941e-14 Identity = 28/59 (47.46%), Postives = 43/59 (72.88%), Query Frame = 3 Query: 213 CQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTS 389 C+ +++ +VLPG GWDNL NIDMGRV++L++S C + D YLIP+E+ + P+K + Sbjct: 33 CRKNSSLTALEVLPGGGWDNLRNIDMGRVMNLSYSQCQTTEDGVYLIPDEVFVIPQKKT 91
BLAST of MACPF domain-containing protein vs. Ensembl Mouse
Match: Pfpl (pore forming protein-like [Source:MGI Symbol;Acc:MGI:1860266]) HSP 1 Score: 91.2781 bits (225), Expect = 2.552e-20 Identity = 47/113 (41.59%), Postives = 71/113 (62.83%), Query Frame = 3 Query: 204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +Q CQ +VLPG GWDNL N+DMG+V+DL ++ C + D +Y+IP+++ P+K S +E++SEI + NY S+TS SINL ++ ++ G FS E +IK Q Sbjct: 31 FQKCQNALNLPVLEVLPGGGWDNLRNMDMGQVMDLTYNNCKTTEDGQYIIPDDVFTIPQKESNLEMNSEILDSWENYRSTTSFSINLNLD---YRPRVNGKFSSEFQRIKTTQ 140
BLAST of MACPF domain-containing protein vs. Ensembl Mouse
Match: Mpeg1 (macrophage expressed gene 1 [Source:MGI Symbol;Acc:MGI:1333743]) HSP 1 Score: 90.1225 bits (222), Expect = 6.504e-20 Identity = 48/115 (41.74%), Postives = 71/115 (61.74%), Query Frame = 3 Query: 204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSIN--LAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +Q C+ +VLPG GWDNL N+DMGRV+DL ++ C + D +Y+IP+E+ P+K S +E++SE+ ++NY S+TS SIN LA+ V G FS E ++K Q Sbjct: 38 FQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVN-----GKFSTEFQRMKTLQ 147
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2KJC3|MPEG1_BOVIN (Macrophage-expressed gene 1 protein OS=Bos taurus OX=9913 GN=MPEG1 PE=2 SV=2) HSP 1 Score: 95.1301 bits (235), Expect = 8.074e-21 Identity = 50/113 (44.25%), Postives = 72/113 (63.72%), Query Frame = 3 Query: 204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +QNC+ VLPG GWDNL N+D GRV++L +S C + D +Y++P+E+ P+K S +E++S+I +VNY SSTS SIN+ E S+ K+ G FS E +IK Q Sbjct: 31 FQNCKNALKLPVLPVLPGGGWDNLRNVDTGRVMELAYSHCRTTEDGQYIVPDEIFTIPQKQSNLEMNSKILESWVNYQSSTSNSINM--ELSLFS-KVNGKFSLEFQRIKTLQ 140
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2M385|MPEG1_HUMAN (Macrophage-expressed gene 1 protein OS=Homo sapiens OX=9606 GN=MPEG1 PE=1 SV=1) HSP 1 Score: 92.4337 bits (228), Expect = 6.295e-20 Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 3 Query: 195 KLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 ++ Q C+ +VLPG GWDNL N+DMGRV++L +S C + D +Y+IP+E+ P+K S +E++SEI + NY SSTS SIN E S+ K+ G FS E ++K Q Sbjct: 28 EVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5RBP9|MPEG1_PONAB (Macrophage-expressed gene 1 protein OS=Pongo abelii OX=9601 GN=MPEG1 PE=2 SV=1) HSP 1 Score: 92.4337 bits (228), Expect = 6.355e-20 Identity = 50/116 (43.10%), Postives = 72/116 (62.07%), Query Frame = 3 Query: 195 KLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 ++ Q C+ +VLPG GWDNL N+DMGRV++L +S C + D +Y+IP+E+ P+K S +E++SEI + NY SSTS SIN E S+ K+ G FS E ++K Q Sbjct: 28 EVGVQKCKNALKLPVLEVLPGGGWDNLRNVDMGRVMELTYSNCRTTEDGQYIIPDEIFTIPQKQSNLEMNSEILESWANYQSSTSYSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9WV57|MPEG1_RAT (Macrophage-expressed gene 1 protein OS=Rattus norvegicus OX=10116 GN=Mpeg1 PE=2 SV=2) HSP 1 Score: 92.4337 bits (228), Expect = 6.531e-20 Identity = 51/113 (45.13%), Postives = 72/113 (63.72%), Query Frame = 3 Query: 204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +Q C+ +VLPG GWDNL NIDMGRV+DL ++ C + D +Y+IP+E+ P+K S +E++SEI +VNY S+TS SIN E S+ K+ G FS E ++K Q Sbjct: 31 FQTCKDTLKLPVLEVLPGGGWDNLRNIDMGRVIDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEIRDSWVNYQSTTSFSIN--TELSLFS-KVNGKFSTEFQRMKTLQ 140
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Match: sp|A1L314|MPEG1_MOUSE (Macrophage-expressed gene 1 protein OS=Mus musculus OX=10090 GN=Mpeg1 PE=1 SV=1) HSP 1 Score: 90.1225 bits (222), Expect = 4.533e-19 Identity = 48/115 (41.74%), Postives = 71/115 (61.74%), Query Frame = 3 Query: 204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSIN--LAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +Q C+ +VLPG GWDNL N+DMGRV+DL ++ C + D +Y+IP+E+ P+K S +E++SE+ ++NY S+TS SIN LA+ V G FS E ++K Q Sbjct: 31 FQICKNALKLPVLEVLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLESWMNYQSTTSLSINTELALFSRVN-----GKFSTEFQRMKTLQ 140
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A267EFV7 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003757g1 PE=4 SV=1) HSP 1 Score: 105.145 bits (261), Expect = 3.573e-22 Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3 Query: 240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533 F +PG GWDNL N DMG V DL+F+ C S+D Y++P + LYP K S +E+ SE+ HF NYTSS S SINL G + ++GG S E Q+K Sbjct: 51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A267FTE5 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003757g3 PE=4 SV=1) HSP 1 Score: 105.145 bits (261), Expect = 6.241e-22 Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3 Query: 240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533 F +PG GWDNL N DMG V DL+F+ C S+D Y++P + LYP K S +E+ SE+ HF NYTSS S SINL G + ++GG S E Q+K Sbjct: 51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A1I8IZJ2 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 104.76 bits (260), Expect = 1.039e-21 Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3 Query: 240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533 F +PG GWDNL N DMG V DL+F+ C S+D Y++P + LYP K S +E+ SE+ HF NYTSS S SINL G + ++GG S E Q+K Sbjct: 51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A1I8FUV4 (MACPF domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 104.76 bits (260), Expect = 1.142e-21 Identity = 48/98 (48.98%), Postives = 62/98 (63.27%), Query Frame = 3 Query: 240 FDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIK 533 F +PG GWDNL N DMG V DL+F+ C S+D Y++P + LYP K S +E+ SE+ HF NYTSS S SINL G + ++GG S E Q+K Sbjct: 51 FSSMPGGGWDNLRNKDMGLVADLSFTDCALSDDGMYVLPKNVILYPRKESNIEVMSEVIDHFSNYTSSLSVSINLEASGKIAAFRIGGKLSNEFQQVK 148
BLAST of MACPF domain-containing protein vs. TrEMBL
Match: A0A2B4RFA8 (Macrophage-expressed gene 1 protein OS=Stylophora pistillata OX=50429 GN=Mpeg1 PE=4 SV=1) HSP 1 Score: 103.99 bits (258), Expect = 2.397e-21 Identity = 49/112 (43.75%), Postives = 73/112 (65.18%), Query Frame = 3 Query: 207 QNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +NC L + F+VLPG GWDNL N+ MG V +N+S C S+D KYLIP+++ LYP K S+V + +E++ H+ NY+S+T+KSIN G+ + G++S E +K Q Sbjct: 44 RNCPLASGVYRFEVLPGGGWDNLRNLHMGAVSVMNYSKCKTSDDGKYLIPDDVFLYPVKESKVHVFAEMYDHWNNYSSTTTKSINAEASGTFGFGSVSGSYSSEFESVKKHQ 155
BLAST of MACPF domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000015822.2 (pep primary_assembly:Astyanax_mexicanus-2.0:6:8259512:8262317:1 gene:ENSAMXG00000015374.2 transcript:ENSAMXT00000015822.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.855 bits (159), Expect = 7.305e-12 Identity = 37/85 (43.53%), Postives = 56/85 (65.88%), Query Frame = 3 Query: 288 MGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 MGRV+++++S C + D YLIP+E+ + P+K S+VE++SEI S ++ SSTS SIN +GS + L FS EN ++K Q Sbjct: 1 MGRVMNMSYSQCQTTEDGVYLIPDEVFVIPQKVSKVEMNSEIISSWLEQRSSTSGSIN--ADGSFFSV-LNCKFSAENQRMKTHQ 82
BLAST of MACPF domain-containing protein vs. Ensembl Cavefish
Match: MPEG1 (macrophage expressed 1 [Source:HGNC Symbol;Acc:HGNC:29619]) HSP 1 Score: 65.0846 bits (157), Expect = 1.334e-11 Identity = 38/86 (44.19%), Postives = 56/86 (65.12%), Query Frame = 3 Query: 288 MGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVE-GSVEDLKLGGAFSFENTQIKNDQ 542 MGRV+++++S C + D YLIP+E+ + P+K S+VE++SEI S ++ SSTS SIN V SV + K FS EN ++K Q Sbjct: 1 MGRVMNISYSQCQTTEDGVYLIPDEVFVIPQKVSKVEMNSEIISSWLEQKSSTSSSINADVSFFSVVNAK----FSTENQRMKTHQ 82
BLAST of MACPF domain-containing protein vs. Ensembl Nematostella
Match: EDO28266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6Y8]) HSP 1 Score: 87.4261 bits (215), Expect = 5.302e-22 Identity = 39/78 (50.00%), Postives = 55/78 (70.51%), Query Frame = 3 Query: 243 DVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEG 476 +VLPG GWDNL N+ MG V +N+SLC S+D K+LIP+ + LYP K S+V +E + H+ NY+S+T+KSIN + Sbjct: 1 EVLPGGGWDNLRNLHMGAVSAMNYSLCRSSDDGKFLIPDNVFLYPVKKSKVNTFAEFYDHWNNYSSTTTKSINAEAKA 78
BLAST of MACPF domain-containing protein vs. Ensembl Nematostella
Match: EDO37879 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEK6]) HSP 1 Score: 86.6557 bits (213), Expect = 2.372e-19 Identity = 45/100 (45.00%), Postives = 65/100 (65.00%), Query Frame = 3 Query: 243 DVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 +VLPG GWDNL N+ MG V +N+SLC S+D K+L+P+ + LYP K S+V +E + H+ NY+S+T+KSIN + S + G+FS E +K Q Sbjct: 1 EVLPGGGWDNLRNLHMGAVSAMNYSLCRSSDDGKFLVPDNVFLYPVKKSKVNTFAEFYDHWNNYSSTTTKSINAEAKASFGFGSVSGSFSSEYESVKKHQ 100
BLAST of MACPF domain-containing protein vs. Ensembl Medaka
Match: MPEG1 (macrophage-expressed gene 1 protein [Source:NCBI gene;Acc:101169041]) HSP 1 Score: 78.9518 bits (193), Expect = 2.784e-16 Identity = 43/106 (40.57%), Postives = 63/106 (59.43%), Query Frame = 3 Query: 237 TFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLK----LGGAFSFENTQIKNDQ 542 +VLPG GWDNL N+DMGRV++ ++ C + D YLIP+E+ + P K + VE +SEI S +++ ST+ SIN +D+ L FS E T++K Q Sbjct: 69 ALEVLPGGGWDNLRNMDMGRVMNFSYFQCQTTEDGLYLIPDEVFVVPYKETGVETNSEIISSWLDQKGSTAASIN-------DDVSFLPVLNAKFSVEITRMKTHQ 167
BLAST of MACPF domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000006104.2 (pep primary_assembly:ASM223467v1:9:9652430:9669002:1 gene:ENSORLG00000027013.1 transcript:ENSORLT00000006104.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 78.1814 bits (191), Expect = 5.881e-16 Identity = 46/115 (40.00%), Postives = 66/115 (57.39%), Query Frame = 3 Query: 207 QNCQLDT--TQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLK-LGGAFSFENTQIKNDQ 542 + C+ T + K +VLPG GWDNL N+DMGRV++ + C + D YL P+E+ + P K + VE +SEI S +++ ST+ SIN V DL L FS E ++K Q Sbjct: 29 RQCRASTNLSLKALEVLPGGGWDNLRNMDMGRVMNFGYFQCQTTKDGLYLNPDEVFVVPYKETGVETNSEIISSWLDQKGSTAASIN----DDVSDLPVLNAKFSSEIIRMKTHQ 139
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044680.1 (SMESG000044680.1) HSP 1 Score: 298.516 bits (763), Expect = 1.168e-103 Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 3 Query: 108 MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ Sbjct: 1 MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 145
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044677.1 (SMESG000044677.1) HSP 1 Score: 182.185 bits (461), Expect = 4.507e-54 Identity = 90/146 (61.64%), Postives = 111/146 (76.03%), Query Frame = 3 Query: 105 IMTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 I +L + LF S + + EV +K +YQ C+L + QKTFDVLPG+GWDNLANIDMGR+LDLNFS C FSNDNKYLIPN+LA+YPEK+S+VEISSEIF HF NYTS +S+SIN+ EG K+GG+FS ENT+IK+DQ Sbjct: 3 IQIVLIISLFRS-------AVKGSNEVNTIKDSYQLCRLTSKQKTFDVLPGSGWDNLANIDMGRILDLNFSKCSFSNDNKYLIPNQLAIYPEKSSQVEISSEIFHHFSNYTSMSSQSINVGAEGGYGIFKIGGSFSTENTKIKSDQ 141
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044677.1 (SMESG000044677.1) HSP 1 Score: 182.57 bits (462), Expect = 8.182e-54 Identity = 90/147 (61.22%), Postives = 111/147 (75.51%), Query Frame = 3 Query: 105 IMTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQG 545 I +L + LF S + + EV +K +YQ C+L + QKTFDVLPG+GWDNLANIDMGR+LDLNFS C FSNDNKYLIPN+LA+YPEK+S+VEISSEIF HF NYTS +S+SIN+ EG K+GG+FS ENT+IK+DQ Sbjct: 3 IQIVLIISLFRS-------AVKGSNEVNTIKDSYQLCRLTSKQKTFDVLPGSGWDNLANIDMGRILDLNFSKCSFSNDNKYLIPNQLAIYPEKSSQVEISSEIFHHFSNYTSMSSQSINVGAEGGYGIFKIGGSFSTENTKIKSDQA 142
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044681.1 (SMESG000044681.1) HSP 1 Score: 177.563 bits (449), Expect = 3.680e-51 Identity = 84/126 (66.67%), Postives = 101/126 (80.16%), Query Frame = 3 Query: 165 ILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 + + EV +K YQ CQL Q+TFDVLPG+GWDNLANI MGR+LDLNFS C FSNDNKYLIPN+LA+YPEK+S+VEISSEIF HF NYTS +S+SIN+ EG K+GG+FS ENT+IK+DQ Sbjct: 16 VKGSNEVNTIKDAYQLCQLMPNQRTFDVLPGSGWDNLANIGMGRILDLNFSKCSFSNDNKYLIPNQLAIYPEKSSQVEISSEIFRHFSNYTSMSSQSINVGAEGGYGIFKIGGSFSTENTKIKSDQ 141
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Match: SMESG000044682.1 (SMESG000044682.1) HSP 1 Score: 168.318 bits (425), Expect = 1.314e-47 Identity = 79/113 (69.91%), Postives = 94/113 (83.19%), Query Frame = 3 Query: 204 YQNCQLDTTQKTFDVLPGTGWDNLANIDMGRVLDLNFSLCLFSNDNKYLIPNELALYPEKTSRVEISSEIFSHFVNYTSSTSKSINLAVEGSVEDLKLGGAFSFENTQIKNDQ 542 Y+ C L + Q TFDVLPG+GWDNLAN+ MGR+LDLNFS C FSNDNKYLIPN+LA+YP+KTS+VEISSEIF HF NYTS +S+SINL EG K+GG+FS ENT+IK+DQ Sbjct: 32 YELCHLRSNQLTFDVLPGSGWDNLANVGMGRILDLNFSKCSFSNDNKYLIPNQLAIYPQKTSQVEISSEIFRHFSNYTSMSSQSINLDAEGLFGHWKIGGSFSTENTKIKSDQ 144 The following BLAST results are available for this feature:
BLAST of MACPF domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of MACPF domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of MACPF domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of MACPF domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of MACPF domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of MACPF domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of MACPF domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of MACPF domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of MACPF domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of MACPF domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of MACPF domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of MACPF domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of MACPF domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of MACPF domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030421 ID=SMED30030421|Name=MACPF domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=802bpback to top protein sequence of SMED30030421-orf-1 >SMED30030421-orf-1 ID=SMED30030421-orf-1|Name=SMED30030421-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=147bp MTILWVFLFASIHGRFVYGILNTKEVTNLKLNYQNCQLDTTQKTFDVLPGback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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