Lipase

Overview
NameLipase
Smed IDSMED30030407
Length (bp)2561
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Lipase (SMED30030407) t-SNE clustered cells

Violin plots show distribution of expression levels for Lipase (SMED30030407) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Lipase (SMED30030407) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Lipase (SMED30030407) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030407

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30030407h1SMcG0011859 Contig3418GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
dorsal ventral margin of the whole animalSMED30030407h1SMcG0011859 Contig3418GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30030407h1SMcG0011859 Contig3418GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Lipase vs. Ensembl Human
Match: LIPF (lipase F, gastric type [Source:HGNC Symbol;Acc:HGNC:6622])

HSP 1 Score: 313.153 bits (801), Expect = 5.671e-98
Identity = 172/395 (43.54%), Postives = 245/395 (62.03%), Query Frame = 2
Query:   95 TMLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSS-KRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            TM SL+ V      +GLF        +   PE+ MN++++I  + YP E + V TEDGYIL + RIP+G K S ++ +RPVVFLQHGLL S+  W++N P+ SLAFILAD G+DVWL NSRGN ++ +++ Y  DS +FW FS+DEMA+YDL A+I++++  T   +L Y+GHSQGT I F   S N SL  +I +  ALAPVA + + +S I     +   L     IFG   F P + F + L + VC  +    LC + LF++ G+D+ N N +RL +YL+H PA TS +NM H+ QA +S +F+ YD+G    N  +Y +  PP Y +    VP+AV+ GG D LAD  DV   +    N I  ++ I +YNHLDF+W ++A Q +Y DI+
Sbjct:   16 TMASLISV--LGTTHGLF-----GKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSL--FKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLI-YHKEIPFYNHLDFIWAMDAPQEVYNDIV 400          
BLAST of Lipase vs. Ensembl Human
Match: LIPF (lipase F, gastric type [Source:HGNC Symbol;Acc:HGNC:6622])

HSP 1 Score: 312.768 bits (800), Expect = 5.749e-98
Identity = 172/395 (43.54%), Postives = 245/395 (62.03%), Query Frame = 2
Query:   95 TMLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSS-KRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            TM SL+ V      +GLF        +   PE+ MN++++I  + YP E + V TEDGYIL + RIP+G K S ++ +RPVVFLQHGLL S+  W++N P+ SLAFILAD G+DVWL NSRGN ++ +++ Y  DS +FW FS+DEMA+YDL A+I++++  T   +L Y+GHSQGT I F   S N SL  +I +  ALAPVA + + +S I     +   L     IFG   F P + F + L + VC  +    LC + LF++ G+D+ N N +RL +YL+H PA TS +NM H+ QA +S +F+ YD+G    N  +Y +  PP Y +    VP+AV+ GG D LAD  DV   +    N I  ++ I +YNHLDF+W ++A Q +Y DI+
Sbjct:    6 TMASLISV--LGTTHGLF-----GKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSL--FKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLI-YHKEIPFYNHLDFIWAMDAPQEVYNDIV 390          
BLAST of Lipase vs. Ensembl Human
Match: LIPA (lipase A, lysosomal acid type [Source:HGNC Symbol;Acc:HGNC:6617])

HSP 1 Score: 309.686 bits (792), Expect = 9.665e-97
Identity = 165/370 (44.59%), Postives = 228/370 (61.62%), Query Frame = 2
Query:  179 TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIK-MSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFG-NLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
             DPE  MNV+E+I+ + +P E +LV TEDGYIL + RIP G K  S    +PVVFLQHGLL  S  WV N  + SL FILAD GFDVW+ NSRGN +S KH       ++FW FS+DEMA+YDL ASI ++L+ T  +++ Y+GHSQGT I F   SQ   L  +I    AL PVA +    S +    R+    D L+K +FG  EFLP S F+K L ++VC       LCG+  FLL G++  NLN +R+ +Y  H+PA TS +NM+H++QA +  +F+ +D+G    N ++Y++ +PP Y +    VP AV+ GG+DWLAD  DV   +    N +  +  I  + HLDF+WG++A   +Y  II  
Sbjct:   29 VDPETNMNVSEIISYWGFPSEEYLVETEDGYILCLNRIPHGRKNHSDKGPKPVVFLQHGLLADSSNWVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALGPVASVAFCTSPMAKLGRLP---DHLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHDWLADVYDVNILLTQITN-LVFHESIPEWEHLDFIWGLDAPWRLYNKIINL 394          
BLAST of Lipase vs. Ensembl Human
Match: LIPM (lipase family member M [Source:HGNC Symbol;Acc:HGNC:23455])

HSP 1 Score: 302.753 bits (774), Expect = 6.997e-94
Identity = 158/394 (40.10%), Postives = 241/394 (61.17%), Query Frame = 2
Query:  110 MKVYTFSFIYGLFITNALSHKYET---DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFG-IKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGI-FGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            M+++    +  +F  N  S    T   DPE +MN++E+I    YPCE + V TEDGYILS+ RIP G ++   +  RPVV LQHGL+  +  W++N P+ SL FILAD GFDVW+ NSRGN +S KH     D ++FW FS+DEMA++DL A I ++L  T  +K+ Y+G+SQGT + F   S    L  KI    ALAP+A + H +S    F  +    D+++K +FG  EFL  +RF++ L+ Y+CG      +C + + LL G++T+N+N +R  +Y AHT A TS +N++H++QA  S E + +D+G    NL   ++  P +Y++    VP A++ GG DWL++ +DV   +    N I  ++ I  + H+DF+WG++A   +Y +II
Sbjct:   15 MEMWLLILVAYMFQRNVNSVHMPTKAVDPEAFMNISEIIQHQGYPCEEYEVATEDGYILSVNRIPRGLVQPKKTGSRPVVLLQHGLVGGASNWISNLPNNSLGFILADAGFDVWMGNSRGNAWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVINFILQKTGQEKIYYVGYSQGTTMGFIAFSTMPELAQKIKMYFALAPIATVKHAKSPGTKFLLLP---DMMIKGLFGKKEFLYQTRFLRQLVIYLCGQVILDQICSNIMLLLGGFNTNNMNMSRASVYAAHTLAGTSVQNILHWSQAVNSGELRAFDWGSETKNLEKCNQPTPVRYRVRDMTVPTAMWTGGQDWLSNPEDVKMLLSEVTNLI-YHKNIPEWAHVDFIWGLDAPHRMYNEII 404          
BLAST of Lipase vs. Ensembl Human
Match: LIPK (lipase family member K [Source:HGNC Symbol;Acc:HGNC:23444])

HSP 1 Score: 296.204 bits (757), Expect = 1.020e-91
Identity = 155/367 (42.23%), Postives = 228/367 (62.13%), Query Frame = 2
Query:  179 TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKM-SSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFG-NLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
             +PE  MN++++I+ + YP E + VTT+DGYIL + RIP G      ++ +P V+LQHGL+ S+  W+ N P+ SLAF+LAD+G+DVWL NSRGN +S KH+K    S ++W FS DEMA+YDL A+I +++  T   +L Y+GHSQGT I F   S N  L  KI    ALAPV  + + QS +K    ++    ++  +FG   F P + F + + + VC  K    +C +FLF L+G+D  NLN +RL +YL+H PA TS +NM+H+AQA  S + + +D+G    N+ ++H++ PP Y +    VP A++ GG D +AD  DV   +    N I   + I +YNH+DF  G +A Q IY D+I
Sbjct:   27 ANPEANMNISQIISYWGYPYEEYDVTTKDGYILGIYRIPHGRGCPGRTAPKPAVYLQHGLIASASNWICNLPNNSLAFLLADSGYDVWLGNSRGNTWSRKHLKLSPKSPEYWAFSLDEMAKYDLPATINFIIEKTGQKRLYYVGHSQGTTIAFIAFSTNPELAKKIKIFFALAPVVTVKYTQSPMKKLTTLSR--RVVKVLFGDKMFHPHTLFDQFIATKVCNRKLFRRICSNFLFTLSGFDPQNLNMSRLDVYLSHNPAGTSVQNMLHWAQAVNSGQLQAFDWGNSDQNMMHFHQLTPPLYNITKMEVPTAIWNGGQDIVADPKDVENLLPQIANLIYY-KLIPHYNHVDFYLGEDAPQEIYQDLI 390          
BLAST of Lipase vs. Ensembl Celegans
Match: lipl-1 (Lipase [Source:UniProtKB/TrEMBL;Acc:Q93789])

HSP 1 Score: 311.612 bits (797), Expect = 8.080e-98
Identity = 171/389 (43.96%), Postives = 232/389 (59.64%), Query Frame = 2
Query:  128 SFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSS--SSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNS-SLNSKINSVVALAPVAFLGHVQSGIKYFAR-IAGGLDILLKIFGHGEFLPSSRFMKDLISYVC-GDKAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDV--FAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            + +  +  T A    ++ DPE+ M   ++I  + YP   + VTTEDGYIL + RIP+G    +  + K+PVVF+QHGL  SS  WV N P +S AF+ AD G+DVWL N RGN YS KH       + FWD+SWDEM QYDL A IE  L VT  D L YIGHSQGT  MF++LS++     +KI    ALAPV  + H++  +K+FA   +   D    +FG GEFLP++  MK +   VC G K  A +C D +FL+ G +++ LN TR+PIY+AHTPA TS +N+VH+ Q         YDYG  GN  +Y + + P Y   T   P+ ++WG +DWLAD  DV  F    +  + +  N  +  YNHLDF+WG+ A + IY  II
Sbjct:    6 TVMLAVLATAATVFGHDADPEMKMTTPQIIMRWGYPAMIYDVTTEDGYILELHRIPYGKTNVTWPNGKKPVVFMQHGLECSSSNWVVNLPTESAAFLFADAGYDVWLGNFRGNTYSMKHKNLKPSHSAFWDWSWDEMQQYDLPAMIEKALEVTGQDSLYYIGHSQGTLTMFSRLSEDKVGWGNKIKKFFALAPVGSVKHIKGALKFFADYFSLEFDGWFDVFGSGEFLPNNWIMKLVSESVCAGLKVEAGVCDDVMFLIAGPESNQLNATRVPIYVAHTPAGTSTQNIVHWIQMVRHGGTPKYDYGEKGNKKHYGQANVPAYDFTTVNRPVYLYWGDSDWLADPTDVTDFLLTHLNPSTVVQNNKLIDYNHLDFIWGLRAPKDIYEPII 394          
BLAST of Lipase vs. Ensembl Celegans
Match: lipl-5 (Lipase [Source:UniProtKB/TrEMBL;Acc:O61866])

HSP 1 Score: 293.508 bits (750), Expect = 4.699e-91
Identity = 163/391 (41.69%), Postives = 221/391 (56.52%), Query Frame = 2
Query:  131 FIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSS--SSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQ-NSSLNSKINSVVALAPVAFLGHVQSGIKYFAR-IAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD-KAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNT-HGVPLAVFWGGNDWLADYDDV--FAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            F+   F+ + +      DPE++M   ++I  + YP   + V T+DGYIL M RIPFG    +  + KRPVVF+QHGLL +S  WV N PD+S  F+ AD GFDVWL N RGN YS KH       + FWD+SWDEMA YDL+A I +VL VT  D + Y+GHSQGT  MF+ LS+ + S   KI    ALAP+  + H++  + +FA   +   D    IFG GEFLP++  MK     +CG  K  A LC + LFL+ G ++   N TR+P+Y  H PA TS +N+VH+ Q         YD+G   N   Y + +PP+Y      G  + ++W   DWLAD  DV  +   ++    +  N  +  YNHLDF WG+ A   IY   IK 
Sbjct:    7 FLAAFFVQDVVGS--HGDPELHMTTPQIIERWGYPAMIYTVATDDGYILEMHRIPFGKTNVTWPNGKRPVVFMQHGLLCASSDWVVNLPDQSAGFLFADAGFDVWLGNMRGNTYSMKHKDLKPSHSAFWDWSWDEMATYDLNAMINHVLEVTGQDSVYYMGHSQGTLTMFSHLSKDDGSFAKKIKKFFALAPIGSVKHIKGFLSFFANYFSLEFDGWFDIFGAGEFLPNNWAMKLAAKDICGGLKVEADLCDNVLFLIAGPESDQWNQTRVPVYATHDPAGTSTQNIVHWMQMVHHGGVPAYDWGTKTNKKKYGQANPPEYDFTAIKGTKIYLYWSDADWLADTPDVPDYLLTRLNPAIVAQNNHLPDYNHLDFTWGLRAPDDIYRPAIKL 395          
BLAST of Lipase vs. Ensembl Celegans
Match: lipl-5 (Lipase [Source:UniProtKB/TrEMBL;Acc:O61866])

HSP 1 Score: 293.508 bits (750), Expect = 4.699e-91
Identity = 163/391 (41.69%), Postives = 221/391 (56.52%), Query Frame = 2
Query:  131 FIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSS--SSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQ-NSSLNSKINSVVALAPVAFLGHVQSGIKYFAR-IAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD-KAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNT-HGVPLAVFWGGNDWLADYDDV--FAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            F+   F+ + +      DPE++M   ++I  + YP   + V T+DGYIL M RIPFG    +  + KRPVVF+QHGLL +S  WV N PD+S  F+ AD GFDVWL N RGN YS KH       + FWD+SWDEMA YDL+A I +VL VT  D + Y+GHSQGT  MF+ LS+ + S   KI    ALAP+  + H++  + +FA   +   D    IFG GEFLP++  MK     +CG  K  A LC + LFL+ G ++   N TR+P+Y  H PA TS +N+VH+ Q         YD+G   N   Y + +PP+Y      G  + ++W   DWLAD  DV  +   ++    +  N  +  YNHLDF WG+ A   IY   IK 
Sbjct:    7 FLAAFFVQDVVGS--HGDPELHMTTPQIIERWGYPAMIYTVATDDGYILEMHRIPFGKTNVTWPNGKRPVVFMQHGLLCASSDWVVNLPDQSAGFLFADAGFDVWLGNMRGNTYSMKHKDLKPSHSAFWDWSWDEMATYDLNAMINHVLEVTGQDSVYYMGHSQGTLTMFSHLSKDDGSFAKKIKKFFALAPIGSVKHIKGFLSFFANYFSLEFDGWFDIFGAGEFLPNNWAMKLAAKDICGGLKVEADLCDNVLFLIAGPESDQWNQTRVPVYATHDPAGTSTQNIVHWMQMVHHGGVPAYDWGTKTNKKKYGQANPPEYDFTAIKGTKIYLYWSDADWLADTPDVPDYLLTRLNPAIVAQNNHLPDYNHLDFTWGLRAPDDIYRPAIKL 395          
BLAST of Lipase vs. Ensembl Celegans
Match: lipl-6 (LIPase Like [Source:UniProtKB/TrEMBL;Acc:E2S7J2])

HSP 1 Score: 295.049 bits (754), Expect = 2.325e-89
Identity = 153/371 (41.24%), Postives = 221/371 (59.57%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGI----KMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA--PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDV--FAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            DPE  M+V E+I  + YP E H V T DGYIL++ RIP G     K +S + +PVVFLQHGLL +S  W+ N P +S  +I AD G+DVWL N RGN YS +H +  +   +FW FSW+EMA+YDL A I Y L  TK   L Y+GHSQG   MFAK+S++  ++ KI    A+APVA + HV+   +   +I    +++ ++FG GEFL ++ F K L    C D+A    LC +F+F ++G +++  N +R+ IYLAH PA TS++N++H+AQ  +      +D+G   NL  Y    PP+Y +      + +F+   DWLA+  DV  F    +    +K    +  +NH DF+WG+ A + IY  II
Sbjct:  175 DPEALMDVPEIITHWGYPVETHKVVTVDGYILTLHRIPHGKNETSKSASKTPKPVVFLQHGLLCTSSIWLLNLPRQSAGYIFADQGYDVWLGNMRGNTYSKEHTRMTSADRRFWKFSWEEMARYDLPAMINYALKTTKRQNLYYVGHSQGALTMFAKMSEDPEMSKKIRKFFAMAPVARMSHVKGLFQNLGQIYEQYNLVYQVFGDGEFLTNNIFTKLLTDIFC-DQAVNNPLCENFIFAVSGPNSNQFNNSRIGIYLAHNPAGTSSRNILHFAQMVKKKRMSRFDHGKDLNLKIYGAPSPPEYDIRKINSSIYLFYSDFDWLANPKDVEGFLIPMLPSKTLKKATKLRDFNHNDFLWGMRARKEIYDKII 544          
BLAST of Lipase vs. Ensembl Celegans
Match: lipl-5 (Lipase [Source:UniProtKB/TrEMBL;Acc:O61866])

HSP 1 Score: 284.648 bits (727), Expect = 4.484e-88
Identity = 158/369 (42.82%), Postives = 210/369 (56.91%), Query Frame = 2
Query:  197 MNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSS--SSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQ-NSSLNSKINSVVALAPVAFLGHVQSGIKYFAR-IAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD-KAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNT-HGVPLAVFWGGNDWLADYDDV--FAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            M   ++I  + YP   + V T+DGYIL M RIPFG    +  + KRPVVF+QHGLL +S  WV N PD+S  F+ AD GFDVWL N RGN YS KH       + FWD+SWDEMA YDL+A I +VL VT  D + Y+GHSQGT  MF+ LS+ + S   KI    ALAP+  + H++  + +FA   +   D    IFG GEFLP++  MK     +CG  K  A LC + LFL+ G ++   N TR+P+Y  H PA TS +N+VH+ Q         YD+G   N   Y + +PP+Y      G  + ++W   DWLAD  DV  +   ++    +  N  +  YNHLDF WG+ A   IY   IK 
Sbjct:    1 MTTPQIIERWGYPAMIYTVATDDGYILEMHRIPFGKTNVTWPNGKRPVVFMQHGLLCASSDWVVNLPDQSAGFLFADAGFDVWLGNMRGNTYSMKHKDLKPSHSAFWDWSWDEMATYDLNAMINHVLEVTGQDSVYYMGHSQGTLTMFSHLSKDDGSFAKKIKKFFALAPIGSVKHIKGFLSFFANYFSLEFDGWFDIFGAGEFLPNNWAMKLAAKDICGGLKVEADLCDNVLFLIAGPESDQWNQTRVPVYATHDPAGTSTQNIVHWMQMVHHGGVPAYDWGTKTNKKKYGQANPPEYDFTAIKGTKIYLYWSDADWLADTPDVPDYLLTRLNPAIVAQNNHLPDYNHLDFTWGLRAPDDIYRPAIKL 369          
BLAST of Lipase vs. Ensembl Fly
Match: Lip3 (gene:FBgn0023495 transcript:FBtr0082771)

HSP 1 Score: 287.345 bits (734), Expect = 9.318e-89
Identity = 143/363 (39.39%), Postives = 213/363 (58.68%), Query Frame = 2
Query:  200 NVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGI-KYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAP--ALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISY--YNHLDFVWGVNAAQLIYPDIIK 1273
            +  E I    YP E H V T D YIL+M RIP+  K   SS RPV FL HG+L SS  WV   P++SLA++LAD G+DVW+ N+RGN YS  H  +PT    FW+FSW+E+  YD+ A I+YVL+ T   ++ Y+GHSQGT +    +S+    N KI S   L P A++G+++S + + FA I G  + ++++ G  EF+PS++F +DL   +C   +P   +C + +FL+ GYDT  L+Y  L    A +PA  S    +H+ Q Y S +F+ +DY    N Y Y    PP Y+L     P+ +++G NDW+ D  DV        N + L+  + +  + HLDF+WG  A + +Y +++K
Sbjct:   27 DCGERIEDDGYPMERHEVVTSDNYILTMHRIPYSPKTGESSNRPVAFLMHGMLSSSSDWVLMGPERSLAYMLADAGYDVWMGNARGNTYSKAHKYWPTYWQIFWNFSWNEIGMYDVPAMIDYVLAKTGQQQVQYVGHSQGTTVYLVMVSERPEYNDKIKSAHLLGPAAYMGNMKSPLTRAFAPILGQPNAIVEVCGSMEFMPSNKFKQDLGIEMCQATSPYADMCANEIFLIGGYDTEQLDYELLEHIKATSPAGASVNQNLHFCQEYNSGKFRKFDYTALRNPYEYGSYFPPDYKLKNAKAPVLLYYGANDWMCDVSDVRKLRDELPN-MALDYLVPFEKWAHLDFIWGTEARKYVYDEVLK 388          
BLAST of Lipase vs. Ensembl Fly
Match: Lip4 (gene:FBgn0032264 transcript:FBtr0080093)

HSP 1 Score: 278.485 bits (711), Expect = 8.773e-85
Identity = 146/377 (38.73%), Postives = 220/377 (58.36%), Query Frame = 2
Query:  176 ETDPEI----YMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSG-IKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK--APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFI---SYYNHLDFVWGVNAAQLIYPDIIKF 1276
            + DP I    ++N   +I  Y YP ENH + T+DGYIL++ RI      +     PV+ L HGLLDSS TWV   P+K L ++L D G+DVW+AN RGN YS KH+KY T   KFWDF++ EM ++D+ A+++Y+L+ T   +L YIGHSQGT + +   S+      KI  +  LAPVAFL H +S  + + A     + ++LK+ G  EFLP + F+      +C +      +C + +FL TG+D   LN T LP+ + H+PA  S K M H+ Q   S  F+ YD+G   N + Y  I PP Y L      +A+++G NDWLA  +DV    +   N ++  +++     +NHLDF+WG++A +L++  +++ 
Sbjct:   58 DVDPNIQEDSHLNTYSLIKKYGYPAENHTLETDDGYILTLHRI------ARPGATPVL-LVHGLLDSSATWVMMGPNKGLGYLLYDQGYDVWMANVRGNTYSRKHVKYSTHHAKFWDFTFHEMGKHDIPATMDYILNSTGVSQLHYIGHSQGTVVFWIMASEKPEYMDKIILMQGLAPVAFLKHCRSPVVNFLAEWHLSVSLVLKLIGVHEFLPKNEFISMFNRIICDETTITKEICSNVIFLTTGFDKLQLNETMLPVIVGHSPAGASTKQMQHFGQLNRSGGFRQYDHGWLRNHWIYGTIDPPSYHLENVRAKVALYYGQNDWLAPPEDVEMLNRKLPNVVE--KYLVDDKEFNHLDFIWGIDARELLWDRMLEI 425          
BLAST of Lipase vs. Ensembl Fly
Match: Lip4 (gene:FBgn0032264 transcript:FBtr0331672)

HSP 1 Score: 277.715 bits (709), Expect = 2.165e-84
Identity = 146/377 (38.73%), Postives = 220/377 (58.36%), Query Frame = 2
Query:  176 ETDPEI----YMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSG-IKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK--APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFI---SYYNHLDFVWGVNAAQLIYPDIIKF 1276
            + DP I    ++N   +I  Y YP ENH + T+DGYIL++ RI      +     PV+ L HGLLDSS TWV   P+K L ++L D G+DVW+AN RGN YS KH+KY T   KFWDF++ EM ++D+ A+++Y+L+ T   +L YIGHSQGT + +   S+      KI  +  LAPVAFL H +S  + + A     + ++LK+ G  EFLP + F+      +C +      +C + +FL TG+D   LN T LP+ + H+PA  S K M H+ Q   S  F+ YD+G   N + Y  I PP Y L      +A+++G NDWLA  +DV    +   N ++  +++     +NHLDF+WG++A +L++  +++ 
Sbjct:   72 DVDPNIQEDSHLNTYSLIKKYGYPAENHTLETDDGYILTLHRI------ARPGATPVL-LVHGLLDSSATWVMMGPNKGLGYLLYDQGYDVWMANVRGNTYSRKHVKYSTHHAKFWDFTFHEMGKHDIPATMDYILNSTGVSQLHYIGHSQGTVVFWIMASEKPEYMDKIILMQGLAPVAFLKHCRSPVVNFLAEWHLSVSLVLKLIGVHEFLPKNEFISMFNRIICDETTITKEICSNVIFLTTGFDKLQLNETMLPVIVGHSPAGASTKQMQHFGQLNRSGGFRQYDHGWLRNHWIYGTIDPPSYHLENVRAKVALYYGQNDWLAPPEDVEMLNRKLPNVVE--KYLVDDKEFNHLDFIWGIDARELLWDRMLEI 439          
BLAST of Lipase vs. Ensembl Fly
Match: Lip4 (gene:FBgn0032264 transcript:FBtr0304119)

HSP 1 Score: 276.944 bits (707), Expect = 2.680e-84
Identity = 147/376 (39.10%), Postives = 219/376 (58.24%), Query Frame = 2
Query:  176 ETDPEI----YMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK--APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFI---SYYNHLDFVWGVNAAQLIYPDIIKF 1276
            + DP I    ++N   +I  Y YP ENH + T+DGYIL++ RI      +     PV+ L HGLLDSS TWV   P+K L ++L D G+DVW+AN RGN YS KH+KY T   KFWDF++ EM ++D+ A+++Y+L+ T   +L YIGHSQGT + +   S+      KI  +  LAPVAFL H +S +  F      L ++LK+ G  EFLP + F+      +C +      +C + +FL TG+D   LN T LP+ + H+PA  S K M H+ Q   S  F+ YD+G   N + Y  I PP Y L      +A+++G NDWLA  +DV    +   N ++  +++     +NHLDF+WG++A +L++  +++ 
Sbjct:   58 DVDPNIQEDSHLNTYSLIKKYGYPAENHTLETDDGYILTLHRI------ARPGATPVL-LVHGLLDSSATWVMMGPNKGLGYLLYDQGYDVWMANVRGNTYSRKHVKYSTHHAKFWDFTFHEMGKHDIPATMDYILNSTGVSQLHYIGHSQGTVVFWIMASEKPEYMDKIILMQGLAPVAFLKHCRSPVVNFLA-EWHLSLVLKLIGVHEFLPKNEFISMFNRIICDETTITKEICSNVIFLTTGFDKLQLNETMLPVIVGHSPAGASTKQMQHFGQLNRSGGFRQYDHGWLRNHWIYGTIDPPSYHLENVRAKVALYYGQNDWLAPPEDVEMLNRKLPNVVE--KYLVDDKEFNHLDFIWGIDARELLWDRMLEI 423          
BLAST of Lipase vs. Ensembl Fly
Match: CG31871 (gene:FBgn0051871 transcript:FBtr0080104)

HSP 1 Score: 261.151 bits (666), Expect = 2.854e-77
Identity = 139/361 (38.50%), Postives = 210/361 (58.17%), Query Frame = 2
Query:  209 EVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDIL---LKIFGHGEFLPSSRFMKDLISYVCGDKAPA--LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDI-KLNRFISYYNHLDFVWGVNAAQLIYPDIIK 1273
            ++I  Y YP E H V T+DGYIL M RIP          +PV+ L HG+LD+S TWV   P   L ++L+D G+DVW+ NSRGN YS  H    +D  +FWDF++ EM +YDL A+I+Y+LS T  +++ YIGHSQGT I +   S+  +   KI S+ ALAP+A++  ++S +  F  +   LD L    ++    EF+P+++F+ D    VC D A    +C + LFL+ GY++  LN T LP+ L+HTP+  S K + H+ Q  +S  F+ +D G   N   Y+ + PP Y L+   VP+A+++  ND L     V    +   N I K    +  +NHLDF+W ++   L+Y  +++
Sbjct:   81 KLIRKYGYPSETHTVVTKDGYILEMHRIP------KKGAQPVL-LMHGILDTSATWVLMGPKSGLGYMLSDLGYDVWMGNSRGNRYSKNHTSLNSDYQEFWDFTFHEMGKYDLPANIDYILSKTGYEQVHYIGHSQGTAIFWVLCSEQPAYTQKITSMHALAPIAYIHDMKSPL--FRTLVLFLDFLTAATRMLRITEFMPNTKFLVDHSQVVCHDNAMTQDVCSNILFLVAGYNSEQLNKTMLPVMLSHTPSGASIKQLEHFGQLMKSGHFRKFDRGYLRNQLEYNRMTPPDYDLSRVKVPVALYYSVNDLLVSTTGVDRLARELPNVIDKYLVPMERFNHLDFLWAIDVKPLVYNRLVR 432          
BLAST of Lipase vs. Ensembl Zebrafish
Match: lipf (lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2737])

HSP 1 Score: 319.701 bits (818), Expect = 7.033e-101
Identity = 161/366 (43.99%), Postives = 222/366 (60.66%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            DPE+ MN++E+I  + YP E   V TEDGYILS+ RIP G+K  +   +PVVFLQHGLL +   WV N P+ SL F+LAD GFDVW+ NSRGN +S KH+       ++W FS DEMA+ DL A I ++  +T  +++ Y+GHSQGT I F   S    L SKI    ALAPVA +G  +S +   + I   L  +  +FG  +F P +  +K   +  C  K    LCG+  FLL G+D  NLN +R P+Y  H PA TS +NM+H+AQA +S++   YDYG  GN+ +Y++  PP Y +    VP AV+ GG D LAD  DV A +      +   R I ++ HLDF+WG++A Q +Y  +I+ 
Sbjct:   29 DPEVNMNISEIIRHWGYPAEEFEVVTEDGYILSINRIPHGVKNKNEEVKPVVFLQHGLLAAGSNWVTNLPNNSLGFVLADAGFDVWIGNSRGNTWSCKHVSLDPRQKEYWKFSHDEMAKKDLPAVINFITKMTGQEQIFYVGHSQGTTIAFMAFSTMPELASKIKMFFALAPVATVGMTKSPMTKLSVIPEFL--IWDLFGQKDFFPQNELIKFFATEFCSRKPLSVLCGNVFFLLCGFDEKNLNMSRTPVYTTHCPAGTSVQNMIHWAQAVKSSKLMAYDYGRAGNMAHYNQSTPPLYNIQDMTVPTAVWSGGQDTLADPKDV-ALLLTQIPKLVYKRDIKHWEHLDFIWGMDAPQEMYEKMIEI 391          
BLAST of Lipase vs. Ensembl Zebrafish
Match: lipf (lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2737])

HSP 1 Score: 319.701 bits (818), Expect = 7.033e-101
Identity = 161/366 (43.99%), Postives = 222/366 (60.66%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            DPE+ MN++E+I  + YP E   V TEDGYILS+ RIP G+K  +   +PVVFLQHGLL +   WV N P+ SL F+LAD GFDVW+ NSRGN +S KH+       ++W FS DEMA+ DL A I ++  +T  +++ Y+GHSQGT I F   S    L SKI    ALAPVA +G  +S +   + I   L  +  +FG  +F P +  +K   +  C  K    LCG+  FLL G+D  NLN +R P+Y  H PA TS +NM+H+AQA +S++   YDYG  GN+ +Y++  PP Y +    VP AV+ GG D LAD  DV A +      +   R I ++ HLDF+WG++A Q +Y  +I+ 
Sbjct:   29 DPEVNMNISEIIRHWGYPAEEFEVVTEDGYILSINRIPHGVKNKNEEVKPVVFLQHGLLAAGSNWVTNLPNNSLGFVLADAGFDVWIGNSRGNTWSCKHVSLDPRQKEYWKFSHDEMAKKDLPAVINFITKMTGQEQIFYVGHSQGTTIAFMAFSTMPELASKIKMFFALAPVATVGMTKSPMTKLSVIPEFL--IWDLFGQKDFFPQNELIKFFATEFCSRKPLSVLCGNVFFLLCGFDEKNLNMSRTPVYTTHCPAGTSVQNMIHWAQAVKSSKLMAYDYGRAGNMAHYNQSTPPLYNIQDMTVPTAVWSGGQDTLADPKDV-ALLLTQIPKLVYKRDIKHWEHLDFIWGMDAPQEMYEKMIEI 391          
BLAST of Lipase vs. Ensembl Xenopus
Match: lipa (lipase A, lysosomal acid, cholesterol esterase [Source:NCBI gene;Acc:548564])

HSP 1 Score: 312.383 bits (799), Expect = 4.411e-97
Identity = 157/367 (42.78%), Postives = 223/367 (60.76%), Query Frame = 2
Query:  173 YETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            Y  DPE  MN++E+I    YP E + V TEDGYILS+ RIP G+K +S   +PVVFLQHGLL     WV N  + SL FILAD G+DVW+ NSRGN +S KH     D  +FW FS+DE+A+ DL A ++++   T  +++ Y+GHSQGT I F   S    L  KI     LAPVA +   +S +     +   L  + ++FG GEFLP +  +  L ++ C    A  LCG+  FLL+G++  NLN +R+ +Y +H PA TS +NM+H+ Q   S E K +D+G  GN+ +Y++  PP Y +    VP A++ GGNDWLAD  DV   +    N +  ++ I  + HLDF+WG++A + +Y +II
Sbjct:   81 YSADPEATMNISELIRYRGYPSEEYEVLTEDGYILSVNRIPHGVKYASEGPKPVVFLQHGLLADGSNWVTNLENNSLGFILADAGYDVWIGNSRGNTWSRKHKSLSPDQEEFWAFSYDELAKKDLPAVVDFITKKTGQEQIFYVGHSQGTTIAFIAFSSLPQLAKKIKMYFGLAPVATVKFSKSPLAKLGVLPEFL--IEELFGKGEFLPQTYLITWLATHFCTHAIADELCGNIFFLLSGFNEKNLNMSRVNVYSSHCPAGTSVQNMLHWRQGVRSGELKAFDFGTKGNMIHYNQTTPPFYHVRDMTVPTALWTGGNDWLADRKDVALLLTQVSN-LVYHKEIPDWEHLDFIWGLDAPERMYNEII 444          
BLAST of Lipase vs. Ensembl Xenopus
Match: lipa (lipase A, lysosomal acid, cholesterol esterase [Source:NCBI gene;Acc:548564])

HSP 1 Score: 298.901 bits (764), Expect = 3.720e-93
Identity = 153/364 (42.03%), Postives = 220/364 (60.44%), Query Frame = 2
Query:  185 PEIYMNV-TEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            P+  M+  +E+I    YP E + V TEDGYILS+ RIP G+K +S   +PVVFLQHGLL     WV N  + SL FILAD G+DVW+ NSRGN +S KH     D  +FW FS+DE+A+ DL A ++++   T  +++ Y+GHSQGT I F   S    L  KI     LAPVA +   +S +     +   L  + ++FG GEFLP +  +  L ++ C    A  LCG+  FLL+G++  NLN +R+ +Y +H PA TS +NM+H+ Q   S E K +D+G  GN+ +Y++  PP Y +    VP A++ GGNDWLAD  DV   +    N +  ++ I  + HLDF+WG++A + +Y +II
Sbjct:    2 PDCRMHCPSELIRYRGYPSEEYEVLTEDGYILSVNRIPHGVKYASEGPKPVVFLQHGLLADGSNWVTNLENNSLGFILADAGYDVWIGNSRGNTWSRKHKSLSPDQEEFWAFSYDELAKKDLPAVVDFITKKTGQEQIFYVGHSQGTTIAFIAFSSLPQLAKKIKMYFGLAPVATVKFSKSPLAKLGVLPEFL--IEELFGKGEFLPQTYLITWLATHFCTHAIADELCGNIFFLLSGFNEKNLNMSRVNVYSSHCPAGTSVQNMLHWRQGVRSGELKAFDFGTKGNMIHYNQTTPPFYHVRDMTVPTALWTGGNDWLADRKDVALLLTQVSN-LVYHKEIPDWEHLDFIWGLDAPERMYNEII 362          
BLAST of Lipase vs. Ensembl Xenopus
Match: lipa (lipase A, lysosomal acid, cholesterol esterase [Source:NCBI gene;Acc:548564])

HSP 1 Score: 297.745 bits (761), Expect = 9.172e-93
Identity = 151/355 (42.54%), Postives = 215/355 (60.56%), Query Frame = 2
Query:  209 EVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            E+I    YP E + V TEDGYILS+ RIP G+K +S   +PVVFLQHGLL     WV N  + SL FILAD G+DVW+ NSRGN +S KH     D  +FW FS+DE+A+ DL A ++++   T  +++ Y+GHSQGT I F   S    L  KI     LAPVA +   +S +     +   L  + ++FG GEFLP +  +  L ++ C    A  LCG+  FLL+G++  NLN +R+ +Y +H PA TS +NM+H+ Q   S E K +D+G  GN+ +Y++  PP Y +    VP A++ GGNDWLAD  DV   +    N +  ++ I  + HLDF+WG++A + +Y +II
Sbjct:    3 ELIRYRGYPSEEYEVLTEDGYILSVNRIPHGVKYASEGPKPVVFLQHGLLADGSNWVTNLENNSLGFILADAGYDVWIGNSRGNTWSRKHKSLSPDQEEFWAFSYDELAKKDLPAVVDFITKKTGQEQIFYVGHSQGTTIAFIAFSSLPQLAKKIKMYFGLAPVATVKFSKSPLAKLGVLPEFL--IEELFGKGEFLPQTYLITWLATHFCTHAIADELCGNIFFLLSGFNEKNLNMSRVNVYSSHCPAGTSVQNMLHWRQGVRSGELKAFDFGTKGNMIHYNQTTPPFYHVRDMTVPTALWTGGNDWLADRKDVALLLTQVSN-LVYHKEIPDWEHLDFIWGLDAPERMYNEII 354          
BLAST of Lipase vs. Ensembl Xenopus
Match: lipa (lipase A, lysosomal acid, cholesterol esterase [Source:NCBI gene;Acc:548564])

HSP 1 Score: 297.745 bits (761), Expect = 1.157e-92
Identity = 151/358 (42.18%), Postives = 216/358 (60.34%), Query Frame = 2
Query:  200 NVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
             + E+I    YP E + V TEDGYILS+ RIP G+K +S   +PVVFLQHGLL     WV N  + SL FILAD G+DVW+ NSRGN +S KH     D  +FW FS+DE+A+ DL A ++++   T  +++ Y+GHSQGT I F   S    L  KI     LAPVA +   +S +     +   L  + ++FG GEFLP +  +  L ++ C    A  LCG+  FLL+G++  NLN +R+ +Y +H PA TS +NM+H+ Q   S E K +D+G  GN+ +Y++  PP Y +    VP A++ GGNDWLAD  DV   +    N +  ++ I  + HLDF+WG++A + +Y +II
Sbjct:   10 QIFELIRYRGYPSEEYEVLTEDGYILSVNRIPHGVKYASEGPKPVVFLQHGLLADGSNWVTNLENNSLGFILADAGYDVWIGNSRGNTWSRKHKSLSPDQEEFWAFSYDELAKKDLPAVVDFITKKTGQEQIFYVGHSQGTTIAFIAFSSLPQLAKKIKMYFGLAPVATVKFSKSPLAKLGVLPEFL--IEELFGKGEFLPQTYLITWLATHFCTHAIADELCGNIFFLLSGFNEKNLNMSRVNVYSSHCPAGTSVQNMLHWRQGVRSGELKAFDFGTKGNMIHYNQTTPPFYHVRDMTVPTALWTGGNDWLADRKDVALLLTQVSN-LVYHKEIPDWEHLDFIWGLDAPERMYNEII 364          
BLAST of Lipase vs. Ensembl Xenopus
Match: lipa (lipase A, lysosomal acid, cholesterol esterase [Source:NCBI gene;Acc:548564])

HSP 1 Score: 298.13 bits (762), Expect = 1.594e-92
Identity = 151/361 (41.83%), Postives = 219/361 (60.66%), Query Frame = 2
Query:  191 IYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            + ++ +E+I    YP E + V TEDGYILS+ RIP G+K +S   +PVVFLQHGLL     WV N  + SL FILAD G+DVW+ NSRGN +S KH     D  +FW FS+DE+A+ DL A ++++   T  +++ Y+GHSQGT I F   S    L  KI     LAPVA +   +S +     +   L  + ++FG GEFLP +  +  L ++ C    A  LCG+  FLL+G++  NLN +R+ +Y +H PA TS +NM+H+ Q   S E K +D+G  GN+ +Y++  PP Y +    VP A++ GGNDWLAD  DV   +    N +  ++ I  + HLDF+WG++A + +Y +II
Sbjct:   28 LIISSSELIRYRGYPSEEYEVLTEDGYILSVNRIPHGVKYASEGPKPVVFLQHGLLADGSNWVTNLENNSLGFILADAGYDVWIGNSRGNTWSRKHKSLSPDQEEFWAFSYDELAKKDLPAVVDFITKKTGQEQIFYVGHSQGTTIAFIAFSSLPQLAKKIKMYFGLAPVATVKFSKSPLAKLGVLPEFL--IEELFGKGEFLPQTYLITWLATHFCTHAIADELCGNIFFLLSGFNEKNLNMSRVNVYSSHCPAGTSVQNMLHWRQGVRSGELKAFDFGTKGNMIHYNQTTPPFYHVRDMTVPTALWTGGNDWLADRKDVALLLTQVSN-LVYHKEIPDWEHLDFIWGLDAPERMYNEII 385          
BLAST of Lipase vs. Ensembl Mouse
Match: Lipf (lipase, gastric [Source:MGI Symbol;Acc:MGI:1914967])

HSP 1 Score: 301.597 bits (771), Expect = 5.801e-94
Identity = 171/402 (42.54%), Postives = 249/402 (61.94%), Query Frame = 2
Query:   77 FILLSKTMLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSS-SKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            ++LL  ++LS      F   +GLF    L  K   +PE  MNV+++I  + YP E + V TEDGYIL + RIP+G K S +  KRPV +LQHGL+ S+  W+ N P+ SLAFILAD G+DVWL NSRGN +S K++ Y  DS +FW FS+DEMA+YDL A+I++++  T  +K+ Y+GHSQGT I F   S N +L  KI    ALAPVA + + +S  K  + I      LLK IFG+  F+P +   + L + VC  +    LC + LF+  G+D  NLN +R  +YL H PA TS +++ H+AQ  +S + + Y++G    N+ +Y++  PP Y ++   VP+AV+ GG+D LAD  DV   +    N +  ++ I  YNHLDF+W ++A Q +Y +I+
Sbjct:    2 WLLLVTSVLS-----AFGGAHGLF--GKLGPK---NPEANMNVSQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKRPVAYLQHGLIASATNWITNLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIDFIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPFKKISLIP---KFLLKVIFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRFDVYLGHNPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPIAVWNGGHDILADPQDVAMLLPKLPN-LLYHKEILPYNHLDFIWAMDAPQEVYNEIV 389          
BLAST of Lipase vs. Ensembl Mouse
Match: Lipm (lipase, family member M [Source:MGI Symbol;Acc:MGI:1926003])

HSP 1 Score: 299.671 bits (766), Expect = 7.126e-93
Identity = 155/367 (42.23%), Postives = 228/367 (62.13%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFG-IKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKI-FGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGI-FGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            DPE +MNV+E+I    YP E + V TEDGYILS+ RIP G  ++     RPVV LQHGLL  +  W++N P+ SL FILAD GFDVW+ NSRGN +S KH     D ++FW FS+DEMA++DL A I ++L  T   K+ Y+G+SQGT + F   S    L  KI    ALAP+A + + +S    F  +    D+++K+ FG  EFL  +RF + L  Y+CG      +C + + LL G++T+N+N +R  +Y+AHTPA TS +N++H++QA  S E + +D+G    N    ++  P +Y++    VP A++ GG DWL++ DDV   +    N I  ++ I  + H+DF+WG++A Q +Y +II
Sbjct:   42 DPEAFMNVSEIIKHKGYPSEEYEVATEDGYILSVNRIPRGQTRLKKEGSRPVVLLQHGLLGDASNWISNLPNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVINFILQKTGQKKVYYVGYSQGTTMGFIAFSTMPELAHKIKMYFALAPIATVKYARSPGTKFLLLP---DMMIKVLFGRQEFLYQTRFFRQLFIYLCGQMILDQICSNIILLLGGFNTNNMNMSRANVYVAHTPAGTSVQNILHWSQAVNSGELRAFDWGSETKNQEKCNQPTPIRYKVRDMMVPTAMWTGGQDWLSNPDDVKTLLSEVTNLI-YHKNIPEWAHVDFIWGLDAPQRVYNEII 404          
BLAST of Lipase vs. Ensembl Mouse
Match: Lipa (lysosomal acid lipase A [Source:MGI Symbol;Acc:MGI:96789])

HSP 1 Score: 295.049 bits (754), Expect = 1.939e-91
Identity = 160/390 (41.03%), Postives = 226/390 (57.95%), Query Frame = 2
Query:  122 TFSFIYGLFITNALSHKYE-TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMS-SSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIF-GNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
             F F  G+ ++   +      DPE+ MNVTE+I  + YP E H V T DGYILS+ RIP G K       RPVV+LQHGLL  S  WV N  + SL F+LAD GFDVW+ NSRGN +S KH       ++FW FS+DEMA+YDL ASI Y+L+ T  +++ Y+GHSQG  I F   SQ   L  KI   + LAPV  L      +    R+    D LLK +FG  +FLP S  +K L  +VC       LC +  FLL G++  NLN +R+ +Y  H PA TS +NM+H+ Q ++  + + +D+G    N ++Y++  PP Y +    +P A++ GG DWLAD +D+   +      +  ++ I  ++HLDF+WG++A   +Y +II  
Sbjct:    7 VFVFTIGILLSRVPTGTVSAVDPEVNMNVTEIIMRWGYPGEEHSVLTGDGYILSIHRIPRGRKNHFGKGPRPVVYLQHGLLADSSNWVTNIDNSSLGFLLADAGFDVWMGNSRGNTWSLKHKTLSVSQDEFWAFSFDEMAKYDLPASINYILNKTGQEQIYYVGHSQGCTIGFIAFSQMPELAKKIKMFLVLAPVLSLNFASGPLLQLGRLP---DPLLKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTHCPAGTSVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGGRDWLADINDITILLTQIPK-LVYHKNIPEWDHLDFIWGLDAPWKLYDEIISL 392          
BLAST of Lipase vs. Ensembl Mouse
Match: Lipa (lysosomal acid lipase A [Source:MGI Symbol;Acc:MGI:96789])

HSP 1 Score: 295.049 bits (754), Expect = 1.939e-91
Identity = 160/390 (41.03%), Postives = 226/390 (57.95%), Query Frame = 2
Query:  122 TFSFIYGLFITNALSHKYE-TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMS-SSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIF-GNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
             F F  G+ ++   +      DPE+ MNVTE+I  + YP E H V T DGYILS+ RIP G K       RPVV+LQHGLL  S  WV N  + SL F+LAD GFDVW+ NSRGN +S KH       ++FW FS+DEMA+YDL ASI Y+L+ T  +++ Y+GHSQG  I F   SQ   L  KI   + LAPV  L      +    R+    D LLK +FG  +FLP S  +K L  +VC       LC +  FLL G++  NLN +R+ +Y  H PA TS +NM+H+ Q ++  + + +D+G    N ++Y++  PP Y +    +P A++ GG DWLAD +D+   +      +  ++ I  ++HLDF+WG++A   +Y +II  
Sbjct:    7 VFVFTIGILLSRVPTGTVSAVDPEVNMNVTEIIMRWGYPGEEHSVLTGDGYILSIHRIPRGRKNHFGKGPRPVVYLQHGLLADSSNWVTNIDNSSLGFLLADAGFDVWMGNSRGNTWSLKHKTLSVSQDEFWAFSFDEMAKYDLPASINYILNKTGQEQIYYVGHSQGCTIGFIAFSQMPELAKKIKMFLVLAPVLSLNFASGPLLQLGRLP---DPLLKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTHCPAGTSVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGGRDWLADINDITILLTQIPK-LVYHKNIPEWDHLDFIWGLDAPWKLYDEIISL 392          
BLAST of Lipase vs. Ensembl Mouse
Match: Lipk (lipase, family member K [Source:MGI Symbol;Acc:MGI:2679259])

HSP 1 Score: 289.271 bits (739), Expect = 2.715e-89
Identity = 154/369 (41.73%), Postives = 226/369 (61.25%), Query Frame = 2
Query:  179 TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMS-SSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            T+PE  MN++E+I+ + YP E H V TEDGYIL   RIP G   S  ++ + VV+LQHGL+ S+  W+ N P+ SLAF+LAD+G+DVWL NSRGN +S  H++    S ++W FSWDEMA+YDL A++  +L  +   +L Y+GHSQGT I F   S N  L  KI    ALAPVA + + +S +K    ++     +L  FG   F   + F + + + VC  K    LC +FLF L+G+D  NLN +RL +YL+ +PA TS +NM+H+AQA  S + + +D+G    N+ +++++ PP Y ++   VP A++ GG D +AD  D    +    N I     I +YNH+DF  G +A Q +Y D+I+ 
Sbjct:   26 TNPEANMNISELISYWGYPYEKHDVITEDGYILGTYRIPHGKGCSRKTAPKAVVYLQHGLIASANNWICNLPNNSLAFLLADSGYDVWLGNSRGNTWSRNHLRLSPKSPQYWAFSWDEMAKYDLPATVNLILEKSGQKQLFYVGHSQGTTIAFIAFSTNPELAKKIRLFFALAPVATVKYTRSPMKKLTTLSRKAVKVL--FGDKMFSTHTWFEQFIATKVCNRKLFHQLCSNFLFSLSGFDPQNLNMSRLDVYLSQSPAGTSVQNMLHWAQAVNSGQLQAFDWGNPDQNMMHFNQLTPPVYNISKMRVPTAMWSGGQDVVADAKDTKNLLPKIANLIYYKE-IPHYNHMDFYLGQDAPQEVYGDLIRM 391          
BLAST of Lipase vs. UniProt/SwissProt
Match: sp|P80035|LIPG_CANLF (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 322.013 bits (824), Expect = 7.770e-101
Identity = 174/387 (44.96%), Postives = 248/387 (64.08%), Query Frame = 2
Query:  128 SFIYGLFITNALSHK-YETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSS-SKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCG-DKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            S I  L  T+ L  K + T+PE+ MN++++I  + YP E + V TEDGYIL + RIP+G K S +  +RPV FLQHGLL S+  W++N P+ SLAFILAD G+DVWL NSRGN ++ +++ Y  DS +FW FS+DEMA+YDL A+I+++L  T  DKL Y+GHSQGT I F   S N  L  +I +  ALAPVA + + ++ +     +   L  L  IFG+  F P   F + L + VC  +    LC + LF++ G+DT NLN +RL +YL+H PA TS +N++H++QA +S +F+ +D+G    N+ +YH+  PP Y L    VP+AV+ GGND LAD  DV   +    N I  +R I  YNHLDF+W ++A Q +Y +I+  
Sbjct:    9 SVISTLGTTHGLFGKLHPTNPEVTMNISQMITYWGYPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFILKKTGQDKLHYVGHSQGTTIGFIAFSTNPKLAKRIKTFYALAPVATVKYTETLLNKLMLVPSFLFKL--IFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDTMNLNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYNLTDMHVPIAVWNGGNDLLADPHDVDLLLSKLPNLI-YHRKIPPYNHLDFIWAMDAPQAVYNEIVSM 392          
BLAST of Lipase vs. UniProt/SwissProt
Match: sp|P07098|LIPG_HUMAN (Gastric triacylglycerol lipase OS=Homo sapiens OX=9606 GN=LIPF PE=1 SV=1)

HSP 1 Score: 312.768 bits (800), Expect = 2.761e-97
Identity = 172/395 (43.54%), Postives = 245/395 (62.03%), Query Frame = 2
Query:   95 TMLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSS-KRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            TM SL+ V      +GLF        +   PE+ MN++++I  + YP E + V TEDGYIL + RIP+G K S ++ +RPVVFLQHGLL S+  W++N P+ SLAFILAD G+DVWL NSRGN ++ +++ Y  DS +FW FS+DEMA+YDL A+I++++  T   +L Y+GHSQGT I F   S N SL  +I +  ALAPVA + + +S I     +   L     IFG   F P + F + L + VC  +    LC + LF++ G+D+ N N +RL +YL+H PA TS +NM H+ QA +S +F+ YD+G    N  +Y +  PP Y +    VP+AV+ GG D LAD  DV   +    N I  ++ I +YNHLDF+W ++A Q +Y DI+
Sbjct:    6 TMASLISV--LGTTHGLF-----GKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSL--FKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLI-YHKEIPFYNHLDFIWAMDAPQEVYNDIV 390          
BLAST of Lipase vs. UniProt/SwissProt
Match: sp|Q93789|LIPL1_CAEEL (Lipase lipl-1 OS=Caenorhabditis elegans OX=6239 GN=lipl-1 PE=2 SV=2)

HSP 1 Score: 311.612 bits (797), Expect = 1.028e-96
Identity = 171/389 (43.96%), Postives = 232/389 (59.64%), Query Frame = 2
Query:  128 SFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSS--SSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNS-SLNSKINSVVALAPVAFLGHVQSGIKYFAR-IAGGLDILLKIFGHGEFLPSSRFMKDLISYVC-GDKAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDV--FAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDII 1270
            + +  +  T A    ++ DPE+ M   ++I  + YP   + VTTEDGYIL + RIP+G    +  + K+PVVF+QHGL  SS  WV N P +S AF+ AD G+DVWL N RGN YS KH       + FWD+SWDEM QYDL A IE  L VT  D L YIGHSQGT  MF++LS++     +KI    ALAPV  + H++  +K+FA   +   D    +FG GEFLP++  MK +   VC G K  A +C D +FL+ G +++ LN TR+PIY+AHTPA TS +N+VH+ Q         YDYG  GN  +Y + + P Y   T   P+ ++WG +DWLAD  DV  F    +  + +  N  +  YNHLDF+WG+ A + IY  II
Sbjct:    6 TVMLAVLATAATVFGHDADPEMKMTTPQIIMRWGYPAMIYDVTTEDGYILELHRIPYGKTNVTWPNGKKPVVFMQHGLECSSSNWVVNLPTESAAFLFADAGYDVWLGNFRGNTYSMKHKNLKPSHSAFWDWSWDEMQQYDLPAMIEKALEVTGQDSLYYIGHSQGTLTMFSRLSEDKVGWGNKIKKFFALAPVGSVKHIKGALKFFADYFSLEFDGWFDVFGSGEFLPNNWIMKLVSESVCAGLKVEAGVCDDVMFLIAGPESNQLNATRVPIYVAHTPAGTSTQNIVHWIQMVRHGGTPKYDYGEKGNKKHYGQANVPAYDFTTVNRPVYLYWGDSDWLADPTDVTDFLLTHLNPSTVVQNNKLIDYNHLDFIWGLRAPKDIYEPII 394          
BLAST of Lipase vs. UniProt/SwissProt
Match: sp|P38571|LICH_HUMAN (Lysosomal acid lipase/cholesteryl ester hydrolase OS=Homo sapiens OX=9606 GN=LIPA PE=1 SV=2)

HSP 1 Score: 309.686 bits (792), Expect = 4.641e-96
Identity = 165/370 (44.59%), Postives = 228/370 (61.62%), Query Frame = 2
Query:  179 TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIK-MSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFG-NLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
             DPE  MNV+E+I+ + +P E +LV TEDGYIL + RIP G K  S    +PVVFLQHGLL  S  WV N  + SL FILAD GFDVW+ NSRGN +S KH       ++FW FS+DEMA+YDL ASI ++L+ T  +++ Y+GHSQGT I F   SQ   L  +I    AL PVA +    S +    R+    D L+K +FG  EFLP S F+K L ++VC       LCG+  FLL G++  NLN +R+ +Y  H+PA TS +NM+H++QA +  +F+ +D+G    N ++Y++ +PP Y +    VP AV+ GG+DWLAD  DV   +    N +  +  I  + HLDF+WG++A   +Y  II  
Sbjct:   29 VDPETNMNVSEIISYWGFPSEEYLVETEDGYILCLNRIPHGRKNHSDKGPKPVVFLQHGLLADSSNWVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALGPVASVAFCTSPMAKLGRLP---DHLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHDWLADVYDVNILLTQITN-LVFHESIPEWEHLDFIWGLDAPWRLYNKIINL 394          
BLAST of Lipase vs. UniProt/SwissProt
Match: sp|Q4R4S5|LICH_MACFA (Lysosomal acid lipase/cholesteryl ester hydrolase OS=Macaca fascicularis OX=9541 GN=LIPA PE=2 SV=1)

HSP 1 Score: 308.145 bits (788), Expect = 1.446e-95
Identity = 163/370 (44.05%), Postives = 229/370 (61.89%), Query Frame = 2
Query:  179 TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIK-MSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFG-NLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
             +PE  MNV+E+I+ + +P E +LV TEDGYIL + RIP G K  S    +PVVFLQHGLL  S  WV N  + SL FILAD GFDVW+ NSRGN +S KH       ++FW FS+DEMA+YDL ASI ++L+ T  +++ Y+GHSQGT I F   SQ   L  +I    ALAPV  +    S +    R+    D+L+K +FG  EFLP S F+K L ++VC       LCG+  FLL G++  NLN +R+ +Y  H+PA TS +NM+H++QA +  +F+ +D+G    N ++Y++ +PP Y +    VP AV+ GG+DWLAD  D+   +    N +  +  I  + HLDF+WG++A   +Y  II  
Sbjct:   29 VNPETNMNVSEIISYWGFPSEEYLVETEDGYILCLNRIPHGRKNHSDKGPKPVVFLQHGLLADSSNWVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALAPVVSVDFCTSPMAKLGRLP---DLLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHDWLADVYDINILLTQITN-LVFHESIPEWEHLDFIWGLDAPWRLYNKIINL 394          
BLAST of Lipase vs. TrEMBL
Match: A0A4V3SDI7 (Lipase OS=Opisthorchis felineus OX=147828 GN=CRM22_008291 PE=3 SV=1)

HSP 1 Score: 378.252 bits (970), Expect = 9.221e-120
Identity = 195/393 (49.62%), Postives = 256/393 (65.14%), Query Frame = 2
Query:  101 LSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD-KAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            LSL+  Y  SFIY        S     DPEI  N T++I  Y +  E H V T DGYIL + R+      +    + VVFLQHGLLDS+ TW+NN  ++SLAFILAD GFDVWL NSRG+ YS KH KY T   +FW+FSWD+MAQ+DL AS+ +VL VT S+ L Y+GHSQG +I  A+ +++  L S I+  VALAPVA+LGH+ S I+Y A  A  ++ +  +FGHGEFLPS+R +  L  ++CG    P +C + ++LL GYD  N N TRLP+Y+AHTPA TSAKNMVHY Q   +++F+ +DYG   NL  Y +  PPKY L+   VP AVF GGNDWLA   DV   I+     +  +     YNHLDFVWG++AA ++YP+++K 
Sbjct:   15 LSLVLGYPISFIYEDLFLEMWS--LSKDPEILENATQIIRNYGFHVEEHYVKTADGYILCLIRMR---NPNIELNKKVVFLQHGLLDSAHTWINNLRNQSLAFILADAGFDVWLGNSRGSTYSRKHEKYDTHHIEFWEFSWDQMAQFDLPASVYHVLQVTGSNTLGYVGHSQGAQIALAQFNRDPELQSHISLFVALAPVAYLGHIASPIRYIAPFARTVERVWDLFGHGEFLPSTRLLHFLAYFLCGRGHIPFVCTNVVYLLAGYDAKNTNLTRLPVYIAHTPAGTSAKNMVHYCQGITTDQFQAFDYGKVKNLEIYGQKTPPKYDLSKFTVPTAVFTGGNDWLAVEKDVDRLIEQIKPAVVSHINFPEYNHLDFVWGMDAAMVLYPEVLKL 402          
BLAST of Lipase vs. TrEMBL
Match: A0A3Q0KCA5 (Lipase OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 370.163 bits (949), Expect = 1.205e-116
Identity = 178/369 (48.24%), Postives = 252/369 (68.29%), Query Frame = 2
Query:  170 KYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD-KAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIK 1273
            K   DPE+Y N+TE+I+   Y  E H VTTEDG+IL + RI       +S ++ VVFLQHGLLDS+ TWVNN P++SL FILADN +DVWL NSRG+ YS+ H     D  +FW+FSWDEM +YDL A++ YVL+ T ++KLSYIGHSQG +I  A   ++  + S I+  +ALAP A+LG ++S I+Y A     ++ +++ FG+GEFLPS + M+ L  ++C   + P +C + ++LL GYD+ N N +RLPIY+AHTPA TS +NMVHY Q   ++ F+ YDYG+  NL  Y++ +PP Y ++   +P+ +++GG DWLA Y D+   IK     I+   +   YNHLDFVWG+NAA+L+YP I++
Sbjct:   39 KSGVDPEVYQNITEIISSKGYKFEEHYVTTEDGFILCIIRI-LPKCNEASGRQKVVFLQHGLLDSAHTWVNNLPEESLGFILADNCYDVWLGNSRGSTYSSNHQYLRPDDKEFWEFSWDEMGKYDLPATLMYVLNHTDAEKLSYIGHSQGCQIALACFDEHPIIQSFIDLFIALAPAAYLGSIKSPIRYIAPFVKTVEPVVEWFGNGEFLPSGKIMQFLALFLCKPHRIPFVCSNIMYLLAGYDSKNTNVSRLPIYVAHTPAGTSVQNMVHYCQGIVTDRFQKYDYGLIKNLQIYNQSYPPLYNISHLKLPIIIYYGGQDWLASYRDIHKLIKQINYTIRSTHYFPDYNHLDFVWGLNAAKLLYPLILE 406          
BLAST of Lipase vs. TrEMBL
Match: G4VKU5 (Lipase OS=Schistosoma mansoni OX=6183 GN=Smp_011000 PE=3 SV=1)

HSP 1 Score: 370.163 bits (949), Expect = 2.213e-116
Identity = 178/369 (48.24%), Postives = 252/369 (68.29%), Query Frame = 2
Query:  170 KYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD-KAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIK 1273
            K   DPE+Y N+TE+I+   Y  E H VTTEDG+IL + RI       +S ++ VVFLQHGLLDS+ TWVNN P++SL FILADN +DVWL NSRG+ YS+ H     D  +FW+FSWDEM +YDL A++ YVL+ T ++KLSYIGHSQG +I  A   ++  + S I+  +ALAP A+LG ++S I+Y A     ++ +++ FG+GEFLPS + M+ L  ++C   + P +C + ++LL GYD+ N N +RLPIY+AHTPA TS +NMVHY Q   ++ F+ YDYG+  NL  Y++ +PP Y ++   +P+ +++GG DWLA Y D+   IK     I+   +   YNHLDFVWG+NAA+L+YP I++
Sbjct:   55 KSGVDPEVYQNITEIISSKGYKFEEHYVTTEDGFILCIIRI-LPKCNEASGRQKVVFLQHGLLDSAHTWVNNLPEESLGFILADNCYDVWLGNSRGSTYSSNHQYLRPDDKEFWEFSWDEMGKYDLPATLMYVLNHTDAEKLSYIGHSQGCQIALACFDEHPIIQSFIDLFIALAPAAYLGSIKSPIRYIAPFVKTVEPVVEWFGNGEFLPSGKIMQFLALFLCKPHRIPFVCSNIMYLLAGYDSKNTNVSRLPIYVAHTPAGTSVQNMVHYCQGIVTDRFQKYDYGLIKNLQIYNQSYPPLYNISHLKLPIIIYYGGQDWLASYRDIHKLIKQINYTIRSTHYFPDYNHLDFVWGLNAAKLLYPLILE 422          
BLAST of Lipase vs. TrEMBL
Match: A7S3Q1 (Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g103440 PE=4 SV=1)

HSP 1 Score: 368.237 bits (944), Expect = 2.979e-116
Identity = 183/371 (49.33%), Postives = 247/371 (66.58%), Query Frame = 2
Query:  170 KYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGI--KMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKL-NRFISYYNHLDFVWGVNAAQLIYPDII 1270
            +   DPEI MNV+++I  + YP EN+ V T+DGYI+S+QRIPFG   K      +PV+F+QHGLL SS  WV N P++SLAFILADN FDVWL N RGN+Y  +H+     S+ FWDFSWDE ++YDL+A I+  L V+    L Y GHSQGT +MFA+ S N  L SKI +  AL PV  +GH++S IKY A     ++ L KIFG  +FLP++  M+ L    C       +C D +F+L G+D S LN TRLP+Y++HTPA TS KNM+HYAQ Y+S +F+MYDYG   N+  Y +  PP+Y ++   VP  ++WGGNDWLAD DDV   +K       + N+ +  + HLDF+WG++AA+L+Y DI+
Sbjct:    1 RAREDPEINMNVSQMIQYHGYPVENYDVITKDGYIISIQRIPFGQNGKCKDVPNKPVIFVQHGLLCSSTNWVANLPNESLAFILADNCFDVWLGNVRGNIYGMRHVNVSIHSDAFWDFSWDEFSKYDLTAMIDKALKVSNVSSLYYAGHSQGTMMMFAESSCNKDLASKIKAHFALGPVTTIGHIESPIKYLANFVPEVEDLFKIFGIHDFLPNNEIMRILAVLFCEPLGIRDVCSDVIFILDGFDQSQLNMTRLPVYISHTPAGTSVKNMIHYAQMYKSKKFEMYDYGK-DNIKRYGQNTPPQYNISAITVPTMLYWGGNDWLADPDDVSLLMKALPPKTLIDNKELKAWQHLDFIWGLDAAELVYDDIV 370          
BLAST of Lipase vs. TrEMBL
Match: C3ZXQ3 (Lipase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_131171 PE=3 SV=1)

HSP 1 Score: 369.007 bits (946), Expect = 6.080e-116
Identity = 183/366 (50.00%), Postives = 241/366 (65.85%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSS-SSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIK 1273
            DPE++MN T++I    YPCE++ V T+DG++L +QRIP+G   +S   +RP +FLQHGLL +S  W+ N  ++SLAFILAD GFDVWL N RGN YS KH+KY  D ++FWDFSWDEMA+YDL A + + L+ T    L Y+GHSQGT I FA LSQ+     K+ +  ALAPV  LGH+ S IKY A+    +  + +IFG  EFLP+S ++  L S++C       C + LFLL G+D   LN TRLP+Y +HTPA TS KNMVH+AQ   SN+F+ YDYG    N   Y++   P Y +     P+A+FWGGNDWLAD  DV AAI    N +  N  I  ++H+DF+WG +A +L Y  IIK
Sbjct:   37 DPEVHMNATQLITSKGYPCEDYTVKTDDGFLLGVQRIPYGRNATSHKDQRPAIFLQHGLLSASTDWILNLANESLAFILADAGFDVWLGNMRGNTYSRKHVKYTPDDDEFWDFSWDEMAKYDLPAMVTFALNKTGQSSLYYVGHSQGTAIAFAHLSQDQEFAKKVKTFFALAPVVTLGHITSPIKYLAQFDDIISAMFRIFGVDEFLPNSWWLDWLASFLCDKSTEKYCENMLFLLVGFDPVQLNETRLPVYFSHTPAGTSTKNMVHFAQMVNSNKFQAYDYGNPDDNKQQYNQPTAPVYPIENMTTPVALFWGGNDWLADPTDVQAAIPHLKN-VVYNSEIKNFDHMDFIWGKDATKL-YDQIIK 400          
BLAST of Lipase vs. Ensembl Cavefish
Match: lipf (lysosomal acid lipase/cholesteryl ester hydrolase-like [Source:NCBI gene;Acc:103040858])

HSP 1 Score: 330.102 bits (845), Expect = 7.314e-105
Identity = 165/374 (44.12%), Postives = 229/374 (61.23%), Query Frame = 2
Query:  164 SHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKM--SSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            S+    DPE+ MN++E+I  + YP E + V TEDGYILS+ RIP+G+K   S ++ RPVVFLQHGLL +   WV N P+ SL F+LAD GFDVWL NSRGN +S KH+    D N++W+FS DEMA  DL A I ++   T  +K+ Y+GHSQGT I F   S    L SKI    ALAPVA +    S +   + +   L  +  +FG  +F P +  +K   +  C  K    LCG+  FLL G+D  NLN TR P+Y  H PA TS +NM H++QA ++ +   YDYG  GN+ +Y++  PP+Y +    VP AV+ GG+D LAD  DV   +    N +  +R I ++ HLDF+WG++A Q ++ ++IK 
Sbjct:   21 SYNEMVDPEVNMNISEIIRYWGYPAEEYEVVTEDGYILSINRIPYGVKNKDSQAAPRPVVFLQHGLLAAGSNWVTNLPNTSLGFVLADAGFDVWLGNSRGNTWSRKHVSLSPDQNEYWEFSHDEMANKDLPAVINFITKTTAEEKIFYVGHSQGTTIAFMAFSTMPLLASKIKMFFALAPVATVAFTSSPMGKLSVLPEAL--IWDLFGRKDFFPQNELIKFFATEFCTKKPISVLCGNIFFLLCGFDERNLNMTRTPVYTTHCPAGTSVQNMAHWSQAVKTGKLMAYDYGKAGNMAHYNQSTPPQYHVQDMTVPTAVWSGGHDTLADPQDVAVLLTQIPN-LMYHRHIEHWEHLDFIWGLDAPQEMFGEMIKL 391          
BLAST of Lipase vs. Ensembl Cavefish
Match: lipf (lysosomal acid lipase/cholesteryl ester hydrolase-like [Source:NCBI gene;Acc:103023710])

HSP 1 Score: 317.775 bits (813), Expect = 3.125e-100
Identity = 166/384 (43.23%), Postives = 232/384 (60.42%), Query Frame = 2
Query:  152 TNALSH-----KYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKM--SSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAP--ALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            T  L+H     +   DPEI+MN++E+I  + YP E + V TEDGYILS+ RIP GIK   S +  +PVVFLQHGLL ++  WV N P+ SL ++LAD GFDVWL NSRGN +S KH+    D N++W FS DE+A+ DL A I ++   T  +K+ Y+GHSQGT I F   S    L SKI     LAPVA +    S I     +   L  +  +FG   F P S  +K   +  C  K P  A C +  F + G++  NLN TR+P+Y++HTPA TS +NMVH++QA +S +   YD+G  GNL +Y ++ P +Y +    VP A++ GG D LAD  DV   +    N ++ +R I ++ H+DF+WG++A Q +Y +IIK 
Sbjct:   12 TPGLAHGWLFWRRRVDPEIHMNISEIIRYWGYPAEEYEVVTEDGYILSINRIPHGIKNQDSRADPKPVVFLQHGLLAAASNWVTNLPNTSLGYLLADAGFDVWLGNSRGNTWSRKHVSLSRDQNEYWRFSRDELAKKDLPAVINFITRTTGQEKIFYVGHSQGTTIAFMAFSTMPELASKIKMFFGLAPVATIAFTTSPITKLRILPEKL--IWGLFGKKYFFPQSSLIKFFATKFC-SKNPFNAFCRNIFFRIHGFERKNLNMTRVPVYISHTPAGTSVRNMVHWSQALKSGKLMAYDFGKAGNLVHYKKLTPYQYNVKDMTVPTALWSGGRDILADPLDVAMLLPQISNLVQ-HRRIEHWGHMDFIWGLDAPQTLYNEIIKM 391          
BLAST of Lipase vs. Ensembl Sea Lamprey
Match: lipf (lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2737])

HSP 1 Score: 309.301 bits (791), Expect = 1.892e-97
Identity = 164/411 (39.90%), Postives = 252/411 (61.31%), Query Frame = 2
Query:   62 VYINNFILLSKTMLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIK--MSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLK-IFGHGEFLPSSRFMKDLISYVCGDKA--PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            V I N +L+    ++L+ ++   F+ G  + +   +   +DPEIYMN++E+I+   YP   + V T DG+ILS+ RIP G+K   + ++ RP V LQHGLL     WV+N    SL FILAD G+DVW+ NSRGN +S+ H     D  +FW +S+DEMA  DL A ++++L+ TK ++L Y+GHSQGT    A  S N  L  KI   + LA V  + H  S +   A++A   D L++ IFG  EFLP +  +K L + VC DKA    LC +  F++ G++  N+N +R+P+Y +H PA TS +NM+H+ QA  S  F+ YDYG ++ N+ +Y++  PP Y ++   VP A++ GG DWLAD  D  A +    N ++ ++ +  + HLDF+WG++A + ++PD++K 
Sbjct:    4 VLIRNMVLIVALHVTLL-LFGVGFVSGRMLPDQ-HNDLRSDPEIYMNISELISSKGYPNVEYKVETADGFILSVNRIPRGLKDNTAGTAVRPAVLLQHGLLAGGSNWVSNFAHDSLGFILADAGYDVWIGNSRGNTWSHHHRTLTPDQEEFWAWSYDEMASLDLPAVVDFILNETKQERLFYVGHSQGTTTALAAFSSNPKLAKKIKLFIGLAAVVNVHHSTSPM---AKLADVPDWLIEAIFGKKEFLPQNDILKWLSAEVC-DKAIIKDLCENVFFVICGFNEKNMNVSRVPVYTSHCPAGTSIQNMLHWRQAARSGRFQAYDYGSLWLNMKHYNQATPPAYNISAMTVPTALWSGGQDWLADPTDTAALLAKIPNLVQ-HKVLKDWEHLDFIWGMDAPKRLFPDMLKL 407          
BLAST of Lipase vs. Ensembl Sea Lamprey
Match: lipf (lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2737])

HSP 1 Score: 191.045 bits (484), Expect = 2.714e-55
Identity = 111/273 (40.66%), Postives = 160/273 (58.61%), Query Frame = 2
Query:  176 ETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA--PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQ 988
             +DPE+YMN++E+I+   YP   + V T DG+ILS+ RIP G+K S+++ RP V LQHGLL     WV+N    SL FILAD G+DVW+ NSRGN  S+ H     D  +FW +S       DL A ++++L+ T+ ++L Y GHSQ T I  A  S N  L  KI   + LA V  + H  S +   A++A   D L+K      FLP +  +K L + +  DK     LC    F++ G++  N+N +R+P+Y +H PA TS +NM+H+ Q
Sbjct:   20 RSDPEMYMNISELISSKGYPNVEYKVETADGFILSVNRIPRGLKHSTAAVRPAVLLQHGLLAGGSNWVSNFAHDSLGFILADAGYDVWIGNSRGNT-SHHHRALTPDQEEFWAWS-----SLDLPAVVDFILNETE-ERLFYAGHSQRTTIALAAFSSNPKLAKKIKLFIGLAAVVNVHHSTSPM---AKLADVPDWLMK------FLPHNDILKWLSAEI-SDKEIIEDLCEHVFFVICGFNEKNMNVSRVPVYASHCPAGTSIQNMLHWRQ 275          
BLAST of Lipase vs. Ensembl Yeast
Match: TGL1 (Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes [Source:SGD;Acc:S000001623])

HSP 1 Score: 182.57 bits (462), Expect = 1.022e-49
Identity = 131/384 (34.11%), Postives = 201/384 (52.34%), Query Frame = 2
Query:  155 NALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPD-KSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGI-KYFARIAGGLDILLKIFGHGEFLPSS----RFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG--IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGN--DIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            N +  K  + P I+    E+  ++    E+HLV TED YIL++ RIP   K   ++K  VV+L HGLL  S  W  N    K+L F+L D G+DVW+ N+RGN YS  H+  P  SNKFWDFS DE A +D+  SIE++L +TK DK+  IG SQG+  MFA  S +  LN K++  +A+AP      + + I    A+ + G   L   FG    LPS+    R +   +  +C D    +    LF    ++   L   ++  Y A   + TS K++VH+ Q   S +F+M++    +  +L   ++I     + N   +P+ + +GG D L D  DV      F +  D+K++     Y HLD +WG +A  L+   +++F
Sbjct:   54 NTIEEKLRSAPTIH----EMCAIFDISVEDHLVRTEDNYILTLHRIPPISKNRFNNK--VVYLHHGLLMCSDVWCCNIERHKNLPFVLHDLGYDVWMGNNRGNKYSTAHLNKPPKSNKFWDFSIDEFAFFDIPNSIEFILDITKVDKVICIGFSQGSAQMFAAFSLSEKLNRKVSHFIAIAPAMTPKGLHNRIVDTLAKSSPGFMYLF--FGRKIVLPSAVIWQRTLHPTLFNLCID----IANKILFNWKSFNI--LPRQKIASY-AKLYSTTSVKSIVHWFQILRSQKFQMFEESDNMLNSLTRPYQIANFPTRTNI-KIPILLIYGGIDSLVDI-DVMKKNLPFNSVFDVKVDN----YEHLDLIWGKDADTLVIAKVLRF 416          
BLAST of Lipase vs. Ensembl Yeast
Match: YEH2 (Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication [Source:SGD;Acc:S000004010])

HSP 1 Score: 97.8265 bits (242), Expect = 1.131e-21
Identity = 73/214 (34.11%), Postives = 108/214 (50.47%), Query Frame = 2
Query:  224 YKYPCENHLVTTEDGYILSM----QRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIK-YPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKL-------SQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLI 829
            Y    E   V T+DG+I+ +     R+  G++     KR  + L HGLL S   + ++   KSLA+ L ++GFDVWL N+R  + +  ++K    D +K WD+   +M QYDL A I YVL  T   KLS + HSQGT   F  L       + +  L  K+ + VALAP  + G +     +   +A G+D     FG   F+P    M+ L+
Sbjct:  157 YGIDIEEFEVETDDGFIIDLWHFKSRLNDGVE---EVKREPILLLHGLLQSCGAFASSG-RKSLAYFLYESGFDVWLGNNRCGLNAKWNMKKLGNDHSKKWDWDMHQMVQYDLKALINYVLDSTGYAKLSLVAHSQGTTQGFMGLVNGEKLYASDFKLVDKLENFVALAPAVYPGPLLDEKAFVRLMAKGIDSPW-YFGRRSFIPLMMTMRKLM 365          
BLAST of Lipase vs. Ensembl Yeast
Match: YEH1 (Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication [Source:SGD;Acc:S000003935])

HSP 1 Score: 95.9005 bits (237), Expect = 5.271e-21
Identity = 65/180 (36.11%), Postives = 96/180 (53.33%), Query Frame = 2
Query:  218 NLYKYPCENHLVTTEDGYILSM-QRIP-FGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSN-KHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKL------------SQNSSLNSKINSVVALAPVAFLG 712
            N Y    E   + TEDG+++ +   IP +    S   KRP + + HGLL SS ++ +N   KSLA+ L  +G+D+WL N+R          K PT +++ WD+   EM +YDL+  I+ VL+ T+ +KL+ I HSQGT   F  L            S+ S   SKI + +ALAP  + G
Sbjct:  183 NQYNIQIEEFRLETEDGFVIDLWHLIPKYRTTDSDKKKRPPILMLHGLLQSSGSFASNG-RKSLAYFLYQSGYDIWLGNNRCGFRPEWNEAKVPTLASR-WDWDLREMVKYDLTLLIDTVLAKTQFEKLTLISHSQGTTQGFMGLVNEDKFFPPGSGSKESFFTSKIANYIALAPAVYPG 360          
BLAST of Lipase vs. Ensembl Nematostella
Match: EDO41646 (Lipase [Source:UniProtKB/TrEMBL;Acc:A7S3Q1])

HSP 1 Score: 368.237 bits (944), Expect = 4.120e-120
Identity = 183/371 (49.33%), Postives = 247/371 (66.58%), Query Frame = 2
Query:  170 KYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGI--KMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKA-PALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKL-NRFISYYNHLDFVWGVNAAQLIYPDII 1270
            +   DPEI MNV+++I  + YP EN+ V T+DGYI+S+QRIPFG   K      +PV+F+QHGLL SS  WV N P++SLAFILADN FDVWL N RGN+Y  +H+     S+ FWDFSWDE ++YDL+A I+  L V+    L Y GHSQGT +MFA+ S N  L SKI +  AL PV  +GH++S IKY A     ++ L KIFG  +FLP++  M+ L    C       +C D +F+L G+D S LN TRLP+Y++HTPA TS KNM+HYAQ Y+S +F+MYDYG   N+  Y +  PP+Y ++   VP  ++WGGNDWLAD DDV   +K       + N+ +  + HLDF+WG++AA+L+Y DI+
Sbjct:    1 RAREDPEINMNVSQMIQYHGYPVENYDVITKDGYIISIQRIPFGQNGKCKDVPNKPVIFVQHGLLCSSTNWVANLPNESLAFILADNCFDVWLGNVRGNIYGMRHVNVSIHSDAFWDFSWDEFSKYDLTAMIDKALKVSNVSSLYYAGHSQGTMMMFAESSCNKDLASKIKAHFALGPVTTIGHIESPIKYLANFVPEVEDLFKIFGIHDFLPNNEIMRILAVLFCEPLGIRDVCSDVIFILDGFDQSQLNMTRLPVYISHTPAGTSVKNMIHYAQMYKSKKFEMYDYGK-DNIKRYGQNTPPQYNISAITVPTMLYWGGNDWLADPDDVSLLMKALPPKTLIDNKELKAWQHLDFIWGLDAAELVYDDIV 370          
BLAST of Lipase vs. Ensembl Nematostella
Match: EDO35549 (Lipase [Source:UniProtKB/TrEMBL;Acc:A7SL62])

HSP 1 Score: 348.977 bits (894), Expect = 3.446e-112
Identity = 176/375 (46.93%), Postives = 245/375 (65.33%), Query Frame = 2
Query:  161 LSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGI--KMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCG-DKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIK 1273
            L H +E  PE++MNVT++I    YP E++ VTTEDGYILS+QRIP+G   K      +PVVFLQHGLL S+  WV N  ++S  FILAD  FDVWL N RGN Y  +H+K P DS+ FWDFS+DEMA+YDL A I++V   T    L Y GHSQGT I F   + N ++  K+ +  ALAPV+ + H+   +KY A ++  ++ L K+ G  +FLP+   M+ L   VC  D    +C DFLFL+ G D S LN TRLPIY++HTPA TS KN+VH+AQ +   +F+MYDYG    N + Y++  PP+Y ++   VP A++WGG+D LAD  DV   +    + +  N+++  ++HLDF+W ++AA L+Y D+I+
Sbjct:   38 LEHWHEL-PEVHMNVTQLIQYNGYPVEDYDVTTEDGYILSVQRIPYGREGKCKGVKDKPVVFLQHGLLCSATNWVTNLYNESFGFILADQCFDVWLGNVRGNTYGKRHVKLPVDSDAFWDFSFDEMAKYDLPAMIDFVTKTTGQASLYYAGHSQGTMIGFIAFAHNPAVIQKVKAFYALAPVSTVSHMGGALKYLAYLSPEIEFLFKVLGVRDFLPTDDVMRVLADLVCRPDYIRVVCSDFLFLIAGMDRSQLNETRLPIYISHTPAGTSVKNVVHFAQIFREKKFQMYDYGSAEKNKHKYNQDTPPQYNVSAVKVPSALYWGGHDVLADPTDVKDLLAKLPHQM-YNKYLPTWDHLDFIWALDAASLVYDDVIR 410          
BLAST of Lipase vs. Ensembl Nematostella
Match: EDO29518 (Lipase [Source:UniProtKB/TrEMBL;Acc:A7T3E6])

HSP 1 Score: 340.887 bits (873), Expect = 1.806e-109
Identity = 171/367 (46.59%), Postives = 237/367 (64.58%), Query Frame = 2
Query:  185 PEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGI--KMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCG-DKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYG-IFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIK 1273
            PE  M  T++I    YP E++ VTTEDGYILS+QRIP+G   K      +PVVFLQHGLL S+  WV N  ++S  FILAD  FDVWL N RGN Y  +H+K P DS+ FWDFS+DEMA+YDL A I++V   T    L Y GHSQGT I F   + N ++  K+ +  ALAPV+ + H+   +KY A ++  ++ L K+ G  +FLP+   M+ L   VC  D    +C DFLFL+ G D S LN TRLPIY++HTPA TS KN+VH+AQ +   +F+MYDYG    N + Y++  PP+Y ++   VP A++WGG+D LAD  DV   +    + +  N+++  ++HLDF+W ++AA L+Y D+I+
Sbjct:   17 PETNMIFTQLIQYNGYPVEDYDVTTEDGYILSVQRIPYGREGKCKGVKDKPVVFLQHGLLCSATNWVTNLYNESFGFILADQCFDVWLGNVRGNTYGKRHVKLPVDSDAFWDFSFDEMAKYDLPAMIDFVTKTTGQASLYYAGHSQGTMIGFIAFAHNPAVIQKVKAFYALAPVSTVSHMGGALKYLAYLSPEIEFLFKVLGVRDFLPTDDLMRVLADLVCRPDYIRVVCSDFLFLIAGMDRSQLNETRLPIYISHTPAGTSVKNVVHFAQIFREKKFQMYDYGSAEKNKHKYNQDTPPQYNVSAVKVPSALYWGGHDVLADPTDVKDLLAKLPHQM-YNKYLPTWDHLDFIWALDAASLVYDDVIR 382          
BLAST of Lipase vs. Ensembl Nematostella
Match: EDO40744 (Lipase [Source:UniProtKB/TrEMBL;Acc:A7S6G4])

HSP 1 Score: 286.574 bits (732), Expect = 2.332e-88
Identity = 170/398 (42.71%), Postives = 234/398 (58.79%), Query Frame = 2
Query:  131 FIYGLFITNALS--HKYET------DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIK--MSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPS--SRFMKDLISYVCGD-KAPALC---GDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            F+  L+I +AL   H+  T      DP+I  N +++I    YP E H VTT DG+IL++QRIP G       S ++PVVFLQHGLL  S  WV N+P  SL +ILAD GFDVWL N RGN YS  H+K+  DS+KFWD++W +MAQYDL A I+YV   T   ++ Y+GHSQGT I F   S N  L  KI    ALAPV  + HV   IK  A     +  + +     EF+PS  ++ M D  + VC   K+  LC   G+ LF   G+D+SNLN +R+P+ ++H  + TS KNMVH+ Q   S + + Y+YG F N   Y +I PP Y++    VP  +F G +D LAD  DV   +K    ++  +  I  +NH DF++G++A +L+Y  I+K 
Sbjct:    6 FVTCLYIAHALPFIHREATTDEPPRDPDIDRNASQLIRNRGYPVEEHYVTTSDGFILNLQRIPHGRNELREGSGRKPVVFLQHGLLMDSTNWVLNSPHDSLGYILADKGFDVWLGNIRGNEYSAAHVKWNKDSSKFWDWTWQQMAQYDLPAMIDYVTLATSQSQVFYVGHSQGTLIGFTGFSANQELAKKIKMFFALAPVYTVAHVSEFIKASAYALFPVTHIFQNHVSEEFVPSKLTKMMSD--AGVCSRAKSEELCYKTGETLF---GFDSSNLNMSRVPVIMSHWGSGTSFKNMVHFGQMVTSGKCQKYNYGYFYNWMKYGQIDPPHYRVKDMDVPTVLFSGSHDTLADPLDV-GELKPRIQNLVHSEEIPGWNHADFLFGMDAERLLYRKIVKM 397          
BLAST of Lipase vs. Ensembl Nematostella
Match: EDO38398 (Lipase [Source:UniProtKB/TrEMBL;Acc:A7SCY7])

HSP 1 Score: 258.84 bits (660), Expect = 2.572e-78
Identity = 145/359 (40.39%), Postives = 206/359 (57.38%), Query Frame = 2
Query:  179 TDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIK-MSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYV--CGDKAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNA 1243
             DP++  NV+++I+   +P E H V T+DGYILS+QRIP G K   S   RPVVFLQHGLL  S  +V +    SL +ILADNG+DVWL N RGN YS  H+KY     +FWDFS++E  +YD+ A IE+ LSV+   +L YIGHSQGT + F   S +  +  K+   +ALAP+  L H  + ++  A   G +  LL   G  +F P   ++  L++ +  CG K  A LC D   L+ G+D  N N +R+P++  H P+ TS KN++H+ Q         +DYG   N+  Y +  PP Y +    VP A+  G +D L+   DV A I+     +     I  +NH+DFV G++A
Sbjct:   31 VDPDVNRNVSQLIHNRGFPVEEHDVITKDGYILSVQRIPHGRKGRESPGPRPVVFLQHGLLADSSCFVQSWEYDSLGYILADNGYDVWLGNIRGNRYSRSHVKYNHKQREFWDFSFEEFGEYDIPAMIEHALSVSGQSQLYYIGHSQGTLVGFISFSTHPEIAKKVKRFIALAPIFHLNHTATIVRDAAFTLGPIQELLFPLGPTQFFPG--YLIKLLTKLGFCGGKYKAKLCYDISELIFGFDDGNANMSRVPVFFTHFPSGTSFKNIIHFGQIVYYGRTARFDYGKRRNMKRYGKPKPPVYDVTKMDVPTALILGTHDNLSTVPDV-APIRAQIPHVTFYEVIPEWNHIDFVAGIDA 386          
BLAST of Lipase vs. Ensembl Medaka
Match: lipf (lysosomal acid lipase/cholesteryl ester hydrolase-like [Source:NCBI gene;Acc:101156001])

HSP 1 Score: 327.02 bits (837), Expect = 1.515e-103
Identity = 161/366 (43.99%), Postives = 224/366 (61.20%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            DPE+ MN++E+I  + YP E H V TEDGYILS+ RIP G+K ++  K P V LQHGLL +   WV N P  SL F+LAD G+DVW+ NSRGN +S +H     D   FW FS+DEMA  DL A I +VL+VT  D++ YIGHSQGT I F   S    L SK+     LAPVA +    S +   + +   L  +  +FG  +FLP S ++K +  ++CG +    LCG+  F+L G+D  NLN TR P+Y  H PA TS +NMVH+AQA    +   +D+G  GN+ +Y++  PP+Y +    VP A+F GG D LAD  DV   +    N +  ++ I ++ HLDF+WG++A + ++P I+K 
Sbjct:   37 DPEVNMNISEIIRRWGYPAEEHEVVTEDGYILSVNRIPSGLKRTAGPK-PAVLLQHGLLAAGSNWVTNPPSSSLGFVLADAGYDVWIGNSRGNTWSKRHRTLTPDQEDFWKFSYDEMALKDLPAVINHVLNVTAQDQIFYIGHSQGTTIAFMAFSALPELASKVKLFFGLAPVATVAFTNSPMTKLSVLPDAL--IWDLFGKRDFLPQSDYIKWMAEHICGKRLLSELCGNIFFVLCGFDEKNLNMTRTPVYTTHCPAGTSVQNMVHWAQAVHGGKLMGFDFGPAGNMKHYNQSTPPEYHVKDMKVPTALFSGGQDTLADPKDVAVLLTQVSN-LVYHQHIQHWEHLDFIWGLDAPEQMFPSILKL 398          
BLAST of Lipase vs. Ensembl Medaka
Match: lipf (lysosomal acid lipase/cholesteryl ester hydrolase-like [Source:NCBI gene;Acc:101156001])

HSP 1 Score: 315.079 bits (806), Expect = 3.369e-99
Identity = 158/369 (42.82%), Postives = 221/369 (59.89%), Query Frame = 2
Query:  173 YETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            Y   P + +  +E+I  + YP E H V TEDGYILS+ RIP G+K ++  K P V LQHGLL +   WV N P  SL F+LAD G+DVW+ NSRGN +S +H     D   FW FS+DEMA  DL A I +VL+VT  D++ YIGHSQGT I F   S    L SK+     LAPVA +    S +   + +   L  +  +FG  +FLP S ++K +  ++CG +    LCG+  F+L G+D  NLN TR P+Y  H PA TS +NMVH+AQA    +   +D+G  GN+ +Y++  PP+Y +    VP A+F GG D LAD  DV   +    N +  ++ I ++ HLDF+WG++A + ++P I+K 
Sbjct:   23 YRLLPCVCVVQSEIIRRWGYPAEEHEVVTEDGYILSVNRIPSGLKRTAGPK-PAVLLQHGLLAAGSNWVTNPPSSSLGFVLADAGYDVWIGNSRGNTWSKRHRTLTPDQEDFWKFSYDEMALKDLPAVINHVLNVTAQDQIFYIGHSQGTTIAFMAFSALPELASKVKLFFGLAPVATVAFTNSPMTKLSVLPDAL--IWDLFGKRDFLPQSDYIKWMAEHICGKRLLSELCGNIFFVLCGFDEKNLNMTRTPVYTTHCPAGTSVQNMVHWAQAVHGGKLMGFDFGPAGNMKHYNQSTPPEYHVKDMKVPTALFSGGQDTLADPKDVAVLLTQVSN-LVYHQHIQHWEHLDFIWGLDAPEQMFPSILKL 387          
BLAST of Lipase vs. Ensembl Medaka
Match: lipf (lysosomal acid lipase/cholesteryl ester hydrolase-like [Source:NCBI gene;Acc:101156001])

HSP 1 Score: 313.153 bits (801), Expect = 7.236e-99
Identity = 156/362 (43.09%), Postives = 218/362 (60.22%), Query Frame = 2
Query:  194 YMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDK-APALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            + +  E+I  + YP E H V TEDGYILS+ RIP G+K ++  K P V LQHGLL +   WV N P  SL F+LAD G+DVW+ NSRGN +S +H     D   FW FS+DEMA  DL A I +VL+VT  D++ YIGHSQGT I F   S    L SK+     LAPVA +    S +   + +   L  +  +FG  +FLP S ++K +  ++CG +    LCG+  F+L G+D  NLN TR P+Y  H PA TS +NMVH+AQA    +   +D+G  GN+ +Y++  PP+Y +    VP A+F GG D LAD  DV   +    N +  ++ I ++ HLDF+WG++A + ++P I+K 
Sbjct:    5 HTHTLEIIRRWGYPAEEHEVVTEDGYILSVNRIPSGLKRTAGPK-PAVLLQHGLLAAGSNWVTNPPSSSLGFVLADAGYDVWIGNSRGNTWSKRHRTLTPDQEDFWKFSYDEMALKDLPAVINHVLNVTAQDQIFYIGHSQGTTIAFMAFSALPELASKVKLFFGLAPVATVAFTNSPMTKLSVLPDAL--IWDLFGKRDFLPQSDYIKWMAEHICGKRLLSELCGNIFFVLCGFDEKNLNMTRTPVYTTHCPAGTSVQNMVHWAQAVHGGKLMGFDFGPAGNMKHYNQSTPPEYHVKDMKVPTALFSGGQDTLADPKDVAVLLTQVSN-LVYHQHIQHWEHLDFIWGLDAPEQMFPSILKL 362          
BLAST of Lipase vs. Planmine SMEST
Match: SMESG000007797.1 (SMESG000007797.1)

HSP 1 Score: 808.135 bits (2086), Expect = 0.000e+0
Identity = 391/393 (99.49%), Postives = 392/393 (99.75%), Query Frame = 2
Query:   98 MLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            MLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDK+SYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDV AAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF
Sbjct:    1 MLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKISYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVSAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 393          
BLAST of Lipase vs. Planmine SMEST
Match: SMESG000007797.1 (SMESG000007797.1)

HSP 1 Score: 800.431 bits (2066), Expect = 0.000e+0
Identity = 387/389 (99.49%), Postives = 388/389 (99.74%), Query Frame = 2
Query:  110 MKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            MKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDK+SYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDV AAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF
Sbjct:    1 MKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKISYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVSAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 389          
BLAST of Lipase vs. Planmine SMEST
Match: SMESG000074186.1 (SMESG000074186.1)

HSP 1 Score: 489.96 bits (1260), Expect = 9.552e-167
Identity = 224/366 (61.20%), Postives = 285/366 (77.87%), Query Frame = 2
Query:  182 DPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPA-LCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            D EI MNVTE+I  Y YPCENHLVTTEDGYILS+QRIPFG+K +S   RPVVFLQHGL+DSSFTWV N PDKSL F+LAD GFDVWL+N+RGNVYSN HIKY T    FWDF+WD+M +YDL ASI YVL+VTK+ KL YIGHSQGTEI FAK S+NS LNSK+   VALAPV +LGH+++ IKY A +AGGL++L  IF +GEFLP++  +  + ++VCGD+  + LCG+ +FLL G  T+N+N+TR+PIYL+H PA TS KN+VHYAQ++ +NEF+MYDYG F NL  Y +  PPKY+LNT+G+P+A  W G DWL+D +DV  A+   G+++K  +FI  YNH+D +WG  A   ++PD++ F
Sbjct:   27 DSEIKMNVTEIILYYNYPCENHLVTTEDGYILSLQRIPFGLKNNSLQSRPVVFLQHGLVDSSFTWVANGPDKSLGFVLADKGFDVWLSNTRGNVYSNSHIKYTTSDRNFWDFTWDDMVKYDLPASINYVLTVTKAQKLGYIGHSQGTEIAFAKFSENSDLNSKVFGFVALAPVVYLGHLKTAIKYLAGLAGGLNLLANIFTNGEFLPNNELLTTMSTFVCGDQTISILCGNLMFLLYGPGTANMNFTRIPIYLSHVPARTSWKNIVHYAQSHLTNEFQMYDYGFFENLVRYKQAFPPKYRLNTNGLPIAAIWAGADWLSDPEDVSRALNELGDNVKFKKFIPEYNHVDVIWGTTADVKVFPDVVNF 392          
BLAST of Lipase vs. Planmine SMEST
Match: SMESG000074185.1 (SMESG000074185.1)

HSP 1 Score: 488.034 bits (1255), Expect = 6.400e-166
Identity = 227/395 (57.47%), Postives = 301/395 (76.20%), Query Frame = 2
Query:  107 LMKVYTFSFIYGLFIT----NALSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPAL-CGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            + K+     ++GL+      + +    + DPEI+MN T +I LY YP E H V TEDGY L++ RIP+G    S+  R VV+LQHGLLDSSFTW+ N P+KSL FILAD GFDVWL NSRGNVYS  H+KY T SN +WDFSWDEMA++DL AS+++VL VTK+ K+SYIGHSQGT I FAK SQ S+LN +I+S+VA APVA+LG+++SGI+Y A ++GG+D+  K+F HGEFLP++ F K L +YVCG K+ +L C + +FLL+G DTSN+NYTRLP+YL+H PA TS++NM H+AQ Y+SNEFK YD+G+ GNLY+YH+I PP Y LNT+G+PLA+FWGGND+LA   DV  AI  F +D++  +FI  YNH+DF+WG++A +++Y D I F
Sbjct:    1 MTKIVIVFILFGLWTNGKCDDGIDQLAKRDPEIFMNTTAIIRLYGYPVETHKVLTEDGYFLTLHRIPYGRTNMSAENRRVVYLQHGLLDSSFTWLCNLPEKSLGFILADYGFDVWLGNSRGNVYSRGHLKYSTTSNDYWDFSWDEMAEFDLPASLDHVLRVTKAKKISYIGHSQGTAIAFAKFSQYSNLNERISSIVAYAPVAYLGNIRSGIRYLAYVSGGIDLFYKLFRHGEFLPTTIFTKLLANYVCGMKSASLICSNMIFLLSGPDTSNMNYTRLPVYLSHNPAGTSSRNMAHFAQCYKSNEFKKYDHGLLGNLYHYHQISPPTYNLNTNGIPLAMFWGGNDYLAVPQDVSRAIHEFDHDVEFRKFIPSYNHMDFIWGLSAPEVVYKDTIDF 395          
BLAST of Lipase vs. Planmine SMEST
Match: SMESG000074187.1 (SMESG000074187.1)

HSP 1 Score: 470.315 bits (1209), Expect = 4.649e-159
Identity = 221/373 (59.25%), Postives = 280/373 (75.07%), Query Frame = 2
Query:  161 LSHKYETDPEIYMNVTEVINLYKYPCENHLVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDKSLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDLSASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAPVAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGDKAPAL-CGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYESNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDDVFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKF 1276
            LS   ETDPE+ M+V EVI  Y YP E H VTTEDGYIL+MQRIP+G   S   K+PVVFLQHGLLDSSFTWVNN P+KSLAFILAD GFDVWL NSRGN YS KH K+   S  FWDF+WDEMAQ+DL ASI Y L+V+K+DKL YIGHSQGTEI FAK SQ S LNSK+++ VALAPV +LG++++ IKY A +    +++++    GEFLP+ + +  +  +VCGD+  +L CG+  FLL GY+  NLNYTR+PIY+ H PA TS KN+ HYAQ++ SN+F+ +DYG F N   Y++I PP+Y L+THG+P+A+F+GGNDW+A  +DV  AI   G ++KL +FI  YNH+DF+WG NA   IYPD+I F
Sbjct:   22 LSPGIETDPEVTMDVNEVIKYYGYPLETHQVTTEDGYILTMQRIPYGRTSSPGIKKPVVFLQHGLLDSSFTWVNNGPEKSLAFILADRGFDVWLGNSRGNTYSRKHTKFSPFSKFFWDFTWDEMAQFDLPASINYALTVSKADKLGYIGHSQGTEIGFAKFSQFSDLNSKVSAFVALAPVVYLGNLKTAIKYIAGLGDAFNVIMRTVTTGEFLPNIQLVNAMSKFVCGDQMGSLGCGNVAFLLQGYNVENLNYTRMPIYVTHIPAGTSTKNIAHYAQSHLSNKFQKFDYGFFMNKLKYNQITPPQYPLDTHGLPVAMFYGGNDWMAVKEDVTRAIGELGQNVKLIKFIPEYNHIDFIWGANAYTKIYPDVIDF 394          
The following BLAST results are available for this feature:
BLAST of Lipase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
LIPF5.671e-9843.54lipase F, gastric type [Source:HGNC Symbol;Acc:HGN... [more]
LIPF5.749e-9843.54lipase F, gastric type [Source:HGNC Symbol;Acc:HGN... [more]
LIPA9.665e-9744.59lipase A, lysosomal acid type [Source:HGNC Symbol;... [more]
LIPM6.997e-9440.10lipase family member M [Source:HGNC Symbol;Acc:HGN... [more]
LIPK1.020e-9142.23lipase family member K [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Lipase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lipl-18.080e-9843.96Lipase [Source:UniProtKB/TrEMBL;Acc:Q93789][more]
lipl-54.699e-9141.69Lipase [Source:UniProtKB/TrEMBL;Acc:O61866][more]
lipl-54.699e-9141.69Lipase [Source:UniProtKB/TrEMBL;Acc:O61866][more]
lipl-62.325e-8941.24LIPase Like [Source:UniProtKB/TrEMBL;Acc:E2S7J2][more]
lipl-54.484e-8842.82Lipase [Source:UniProtKB/TrEMBL;Acc:O61866][more]
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BLAST of Lipase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lip39.318e-8939.39gene:FBgn0023495 transcript:FBtr0082771[more]
Lip48.773e-8538.73gene:FBgn0032264 transcript:FBtr0080093[more]
Lip42.165e-8438.73gene:FBgn0032264 transcript:FBtr0331672[more]
Lip42.680e-8439.10gene:FBgn0032264 transcript:FBtr0304119[more]
CG318712.854e-7738.50gene:FBgn0051871 transcript:FBtr0080104[more]
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BLAST of Lipase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lipf7.033e-10143.99lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2... [more]
lipf7.033e-10143.99lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2... [more]
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BLAST of Lipase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lipa4.411e-9742.78lipase A, lysosomal acid, cholesterol esterase [So... [more]
lipa3.720e-9342.03lipase A, lysosomal acid, cholesterol esterase [So... [more]
lipa9.172e-9342.54lipase A, lysosomal acid, cholesterol esterase [So... [more]
lipa1.157e-9242.18lipase A, lysosomal acid, cholesterol esterase [So... [more]
lipa1.594e-9241.83lipase A, lysosomal acid, cholesterol esterase [So... [more]
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BLAST of Lipase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lipf5.801e-9442.54lipase, gastric [Source:MGI Symbol;Acc:MGI:1914967... [more]
Lipm7.126e-9342.23lipase, family member M [Source:MGI Symbol;Acc:MGI... [more]
Lipa1.939e-9141.03lysosomal acid lipase A [Source:MGI Symbol;Acc:MGI... [more]
Lipa1.939e-9141.03lysosomal acid lipase A [Source:MGI Symbol;Acc:MGI... [more]
Lipk2.715e-8941.73lipase, family member K [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of Lipase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P80035|LIPG_CANLF7.770e-10144.96Gastric triacylglycerol lipase OS=Canis lupus fami... [more]
sp|P07098|LIPG_HUMAN2.761e-9743.54Gastric triacylglycerol lipase OS=Homo sapiens OX=... [more]
sp|Q93789|LIPL1_CAEEL1.028e-9643.96Lipase lipl-1 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|P38571|LICH_HUMAN4.641e-9644.59Lysosomal acid lipase/cholesteryl ester hydrolase ... [more]
sp|Q4R4S5|LICH_MACFA1.446e-9544.05Lysosomal acid lipase/cholesteryl ester hydrolase ... [more]
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BLAST of Lipase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4V3SDI79.221e-12049.62Lipase OS=Opisthorchis felineus OX=147828 GN=CRM22... [more]
A0A3Q0KCA51.205e-11648.24Lipase OS=Schistosoma mansoni OX=6183 PE=3 SV=1[more]
G4VKU52.213e-11648.24Lipase OS=Schistosoma mansoni OX=6183 GN=Smp_01100... [more]
A7S3Q12.979e-11649.33Predicted protein (Fragment) OS=Nematostella vecte... [more]
C3ZXQ36.080e-11650.00Lipase OS=Branchiostoma floridae OX=7739 GN=BRAFLD... [more]
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BLAST of Lipase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lipf7.314e-10544.12lysosomal acid lipase/cholesteryl ester hydrolase-... [more]
lipf3.125e-10043.23lysosomal acid lipase/cholesteryl ester hydrolase-... [more]
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BLAST of Lipase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lipf1.892e-9739.90lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2... [more]
lipf2.714e-5540.66lipase, gastric [Source:ZFIN;Acc:ZDB-GENE-040426-2... [more]
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BLAST of Lipase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
TGL11.022e-4934.11Steryl ester hydrolase; one of three gene products... [more]
YEH21.131e-2134.11Steryl ester hydrolase; catalyzes steryl ester hyd... [more]
YEH15.271e-2136.11Steryl ester hydrolase; one of three gene products... [more]
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BLAST of Lipase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO416464.120e-12049.33Lipase [Source:UniProtKB/TrEMBL;Acc:A7S3Q1][more]
EDO355493.446e-11246.93Lipase [Source:UniProtKB/TrEMBL;Acc:A7SL62][more]
EDO295181.806e-10946.59Lipase [Source:UniProtKB/TrEMBL;Acc:A7T3E6][more]
EDO407442.332e-8842.71Lipase [Source:UniProtKB/TrEMBL;Acc:A7S6G4][more]
EDO383982.572e-7840.39Lipase [Source:UniProtKB/TrEMBL;Acc:A7SCY7][more]
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BLAST of Lipase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
lipf1.515e-10343.99lysosomal acid lipase/cholesteryl ester hydrolase-... [more]
lipf3.369e-9942.82lysosomal acid lipase/cholesteryl ester hydrolase-... [more]
lipf7.236e-9943.09lysosomal acid lipase/cholesteryl ester hydrolase-... [more]
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BLAST of Lipase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000007797.10.000e+099.49SMESG000007797.1[more]
SMESG000007797.10.000e+099.49SMESG000007797.1[more]
SMESG000074186.19.552e-16761.20SMESG000074186.1[more]
SMESG000074185.16.400e-16657.47SMESG000074185.1[more]
SMESG000074187.14.649e-15959.25SMESG000074187.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030407 ID=SMED30030407|Name=Lipase|organism=Schmidtea mediterranea sexual|type=transcript|length=2561bp
GTATTTTAAGTTTTTTTAATTAAATTTATTAATATCAAATTATTTTTGCA
GTAGTCAATAAGTTTATATCAATAATTTTATATTATTATCTAAAACGATG
TTATCATTAATGAAGGTTTATACTTTCTCATTTATATATGGTTTATTTAT
AACAAATGCTTTGTCACATAAGTATGAAACTGATCCAGAAATTTATATGA
ACGTAACTGAAGTTATAAATCTTTACAAATATCCATGTGAAAATCACCTG
GTTACGACAGAAGATGGTTATATTTTATCAATGCAACGGATTCCTTTTGG
AATTAAAATGTCATCATCATCCAAGCGCCCTGTAGTGTTCCTTCAGCATG
GGCTCTTGGATTCATCGTTTACTTGGGTTAATAATGCTCCGGATAAAAGT
TTGGCTTTCATTTTAGCCGATAATGGCTTTGATGTATGGCTAGCTAATAG
TCGGGGAAATGTCTATTCAAATAAGCACATAAAATATCCCACTGATTCCA
ATAAGTTTTGGGATTTTTCTTGGGATGAAATGGCACAATATGATTTATCG
GCATCAATTGAATATGTATTATCAGTTACGAAATCAGATAAACTAAGTTA
TATTGGACATTCCCAAGGAACAGAAATAATGTTTGCTAAACTTTCACAAA
ATTCATCATTGAATTCGAAAATTAATTCTGTTGTTGCTCTGGCTCCTGTT
GCATTTCTGGGACATGTTCAGAGTGGAATTAAATACTTTGCACGTATTGC
TGGTGGTCTTGATATTTTACTTAAAATATTTGGTCACGGTGAATTTTTGC
CAAGCAGCAGATTTATGAAGGATTTAATTAGTTATGTTTGTGGGGACAAA
GCTCCAGCCCTCTGTGGAGATTTCCTATTTCTACTGACAGGATATGATAC
ATCTAATTTGAATTATACTAGATTACCTATATATCTTGCGCATACTCCAG
CTGAGACGTCAGCAAAAAATATGGTTCATTATGCTCAAGCTTATGAATCA
AACGAGTTCAAAATGTATGATTATGGAATATTCGGAAATTTATATTACTA
CCATGAAATTCATCCACCTAAGTACCAATTGAATACACATGGAGTACCAC
TTGCCGTATTTTGGGGTGGGAATGATTGGTTAGCTGATTATGATGATGTT
TTTGCCGCGATAAAAATTTTTGGAAACGATATAAAACTTAATAGATTTAT
ATCGTATTATAATCATTTGGATTTTGTATGGGGTGTAAATGCTGCTCAAC
TCATATATCCCGATATCATCAAATTTATCAACTCTTATCGAAATTAAAAT
TATTTTAATAATTAAACATTTACTTTGAATTTGATTTGTAATAAAAATAA
ATATTATTGAGCTATAACATGATAATGACAGGTTTTGTTTAAACGTAATA
GATAACTAGCAAAATTTTTACTTCAGTTTCGGCCCTATGATTTCTGTATT
CTACTCCTCAGAAATTATGACTCCTGTAAAAATTTGCTAATTTTCTCTGG
CAGTTTAATGGGTCCACAACGCCCATGAATATATTTTCTTTTCGTAATTG
AAACATTTAAAAAATTAATTTTCAAAAAAATTATTTATAGTTTATGAATT
ACTACCAAGACTGTAAAATACACCTCGACAAAATTTACAGAATCACTTCT
GTAACACAGTACAATAGATAGTTTTTAGTTAGGTTAGCCTAGGGGCTGTG
TGATCTTCTACTGATCGTTTCCTCTTACAGCTGCGCATACTTGGAGATTT
CTTTTTGTTAGCCTCCATGAAACACCAACTACTTGTCCCTGGTTTATAAG
CAGCACACCAAGAATTTTGACATTGTTGTTATCGCCAATAGCTACTACAT
GTCTTAATATTTAACCACTGACTTTTGCACTTAAAAACATAAACATGTTC
TCTAACATTAGAAATGTTTATTGAGTAATCTGCAATTATTACACTGCTTG
CTGTCGAAAGTGTCGACTAACAGTTCAATTTACTTAAATTCTGAAATTTA
ATGAAAACTGAAATTCATTCAAAGAGGAACTACTAGCTATTAAGTGACTG
TGCTGGAAAGTGAACTAAAGTTTGAAATTTATTATATGAAGAAAAAATAA
GAAGAATTTGCAATTTTGCAACATCATAATAATTCAGCGTCAAGCTTAAT
TTTACTCTTTATGCGGACCCATTAGTTATAATGTTGCGACGTATGTGATA
AAGTCCTAATTTATATTTGAATACAGTGCAATCTTTCTTGGATAGGATGT
TATTGAGTGTTTTAAAGCATGTTTAATAACAATTTAGAAACTCCCGCCGT
AAACCATTGCATGGTTGATTGCCTTCAAAATCACATTACTATCACTGGAC
AGGTGTTGTTTTAGATTTTATTTATTGTTTATTAAAAAAATCTTATTATC
CTTTATTGAAAACTTTGATACTGGTTATATCGAGAAATGCCTTCAATCAA
ACAAGCTCGAGAATTGCTGTGGATACAGAAATTATAGAGGGATTTGAGAA
GATGGATTTCTTTTGGAATCCTATATATGTTCAATTTAATCAGTCAGCAA
AATTATTTGTT
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protein sequence of SMED30030407-orf-1

>SMED30030407-orf-1 ID=SMED30030407-orf-1|Name=SMED30030407-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=400bp
MLSLMKVYTFSFIYGLFITNALSHKYETDPEIYMNVTEVINLYKYPCENH
LVTTEDGYILSMQRIPFGIKMSSSSKRPVVFLQHGLLDSSFTWVNNAPDK
SLAFILADNGFDVWLANSRGNVYSNKHIKYPTDSNKFWDFSWDEMAQYDL
SASIEYVLSVTKSDKLSYIGHSQGTEIMFAKLSQNSSLNSKINSVVALAP
VAFLGHVQSGIKYFARIAGGLDILLKIFGHGEFLPSSRFMKDLISYVCGD
KAPALCGDFLFLLTGYDTSNLNYTRLPIYLAHTPAETSAKNMVHYAQAYE
SNEFKMYDYGIFGNLYYYHEIHPPKYQLNTHGVPLAVFWGGNDWLADYDD
VFAAIKIFGNDIKLNRFISYYNHLDFVWGVNAAQLIYPDIIKFINSYRN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR006693AB_hydrolase_lipase
IPR029058AB_hydrolase
IPR025483Lipase_euk
Vocabulary: biological process
TermDefinition
GO:0006629lipid metabolic process
GO:0016042lipid catabolic process
Vocabulary: molecular function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
GO:0016787hydrolase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 36..96
e-value: 6.3E-22
score: 76.9
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 1..399
e-value: 5.7E-136
score: 451.2
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 22..396
e-value: 1.9E-139
score: 466.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 25..395
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 32..396
NoneNo IPR availablePANTHERPTHR11005:SF100LIPASEcoord: 32..396
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..23
score: 0.511