SMED30030348

Overview
NameSMED30030348
Smed IDSMED30030348
Length (bp)2778
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30030348 (SMED30030348) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30030348 (SMED30030348) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30030348 (SMED30030348) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30030348 (SMED30030348) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030348

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30030348SMESG000007934.1 PL08006A2F07ncbi_smed_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
epidermisSMED30030348SMESG000007934.1 dd_Smed_v4_7528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030348SMESG000007934.1 dd_Smed_v4_7528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030348SMESG000007934.1 dd_Smed_v4_7528_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30030348 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000025:224765..227542 -13881
Homology
[Close]
BLAST of SMED30030348 vs. TrEMBL
Match: A0A1I8HCN5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.532e-7
Identity = 52/189 (27.51%), Postives = 85/189 (44.97%), Query Frame = 3 
Query:  255 LVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYS--VKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDE 815
            ++  GQ KSGKS  I E L G  +  S+S P T   T + YS  +  ++ S+    I      +   ++  LV   D++ E K+ + +   AG    F      IID+PGLNE   +       I    P +++Y+ D+    TV  +   T  I Q+    ++ +L  K D + +TE+     + E E
Sbjct:   87 IIIVGQTKSGKSSFINELLGGTFVAQSQS-PCTARVTRLTYSDRIYVLVRSKTGEQIGDVQYLEKPTIEEDLVKLKDKDRENKELISSTVEAGIKCEFLRHGIDIIDSPGLNENSSLDSLTLGAIDSFLP-IIIYVIDSNTGITVAVQNDLTR-IKQTVLGAEVFYLVTKMDVSKDTEQEANQTAQEIE 272
[Close]
BLAST of SMED30030348 vs. TrEMBL
Match: A0A267FEQ6 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021099g2 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 5.394e-7
Identity = 52/189 (27.51%), Postives = 85/189 (44.97%), Query Frame = 3 
Query:  255 LVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYS--VKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDE 815
            ++  GQ KSGKS  I E L G  +  S+S P T   T + YS  +  ++ S+    I      +   ++  LV   D++ E K+ + +   AG    F      IID+PGLNE   +       I    P +++Y+ D+    TV  +   T  I Q+    ++ +L  K D + +TE+     + E E
Sbjct:   87 IIIVGQTKSGKSSFINELLGGTFVAQSQS-PCTARVTRLTYSDRIYVLVRSKTGEQIGDVQYLEKPTIEEDLVKLKDKDRENKELISSTVEAGIKCEFLRHGIDIIDSPGLNENSSLDSLTLGAIDSFLP-IIIYVIDSNTGITVAVQNDLTR-IKQTVLGAEVFYLVTKMDVSKDTEQEANQTAQEIE 272
[Close]
BLAST of SMED30030348 vs. TrEMBL
Match: A0A1I8HTW6 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 6.515e-7
Identity = 52/189 (27.51%), Postives = 85/189 (44.97%), Query Frame = 3 
Query:  255 LVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYS--VKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDE 815
            ++  GQ KSGKS  I E L G  +  S+S P T   T + YS  +  ++ S+    I      +   ++  LV   D++ E K+ + +   AG    F      IID+PGLNE   +       I    P +++Y+ D+    TV  +   T  I Q+    ++ +L  K D + +TE+     + E E
Sbjct:   87 IIIVGQTKSGKSSFINELLGGTFVAQSQS-PCTARVTRLTYSDRIYVLVRSKTGEQIGDVQYLEKPTIEEDLVKLKDKDRENKELISSTVEAGIKCEFLRHGIDIIDSPGLNENSSLDSLTLGAIDSFLP-IIIYVIDSNTGITVAVQNDLTR-IKQTVLGAEVFYLVTKMDVSKDTEQEANQTAQEIE 272
[Close]
BLAST of SMED30030348 vs. TrEMBL
Match: A0A1I8FT07 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.130e-6
Identity = 48/179 (26.82%), Postives = 81/179 (45.25%), Query Frame = 3 
Query:  255 LVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYS--VKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEK 785
            ++  GQ KSGKS  I E L G  +  S+S P T   T + YS  +  ++ S+    I      +   ++  LV   D++ E  + + +   AG    F      IID+PGLNE   +       I    P +++Y+ D+    TV  +   T  I ++    ++ +L  K D + +TE+
Sbjct:   80 IIIVGQTKSGKSSFINELLGGTFVAQSQS-PCTARVTRLTYSDRIYVLVRSKTGEQIGDVQYLEKPTIEEDLVKLKDEDRENDELISSTVEAGIKCEFLRHGIDIIDSPGLNENSSLDSLTLGAIDSFLP-IIIYVIDSNSGITVAVQKDLTT-IKKTVLGAEVFYLVTKMDVSKDTEQ 255
[Close]
BLAST of SMED30030348 vs. Ensembl Nematostella
Match: EDO49816 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG14])

HSP 1 Score: 49.2914 bits (116), Expect = 8.055e-6
Identity = 32/138 (23.19%), Postives = 60/138 (43.48%), Query Frame = 3 
Query:  255 LVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKP-IMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDA 665
            L+  GQ   GKS  I EFL  +V+ P+++ P T     + YS  P +   +     L+  E     +  + +     + + ++ +     AG    F +    +ID+PG +E   + +     +K + P  V+Y+ D 
Sbjct:  104 LIVTGQTNCGKSSIINEFLGARVV-PTKAQPCTARIVRMTYSETPYVRLVKRDGTELERRELKSNKIPREFIELNIDDRDDEKRVNATVEAGLCVDFLKAGIDVIDSPGTSENDVLDELVRNQMKSILP-FVIYVVDG 239
[Close]
BLAST of SMED30030348 vs. Planmine SMEST
Match: SMESG000007934.1 (SMESG000007934.1)

HSP 1 Score: 1659.81 bits (4297), Expect = 0.000e+0
Identity = 883/883 (100.00%), Postives = 883/883 (100.00%), Query Frame = 3 
Query:   63 MMLNSFNVEVKAAKTGIQNQCKIYNENYXXXXXXXXXXXXXXXDDETKAKCRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVLIGYSSKLVRTWRLTHENELPFLLKKHLTFLFXXXXXXXXXXXXXXXXLFEYCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIKIQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRYNNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLHFVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVEDYIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWLMLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEINSKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELLPKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVKLTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSWTVLIVLFGRCQXXXXXXXXXXXXYKDELDEKFSKVNKNMNVFKTCQNMIYYSLKRPYEENIGNSVYKSFNILKKEFENESDIQ 2711
            MMLNSFNVEVKAAKTGIQNQCKIYNENYLLFKKFYEKLLLSKFDDETKAKCRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVLIGYSSKLVRTWRLTHENELPFLLKKHLTFLFEIENQINITAINTIIELFEYCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIKIQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRYNNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLHFVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVEDYIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWLMLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEINSKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELLPKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVKLTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSWTVLIVLFGRCQNLINGNILNNKNYKDELDEKFSKVNKNMNVFKTCQNMIYYSLKRPYEENIGNSVYKSFNILKKEFENESDIQ
Sbjct:    1 MMLNSFNVEVKAAKTGIQNQCKIYNENYLLFKKFYEKLLLSKFDDETKAKCRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVLIGYSSKLVRTWRLTHENELPFLLKKHLTFLFEIENQINITAINTIIELFEYCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIKIQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRYNNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLHFVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVEDYIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWLMLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEINSKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELLPKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVKLTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSWTVLIVLFGRCQNLINGNILNNKNYKDELDEKFSKVNKNMNVFKTCQNMIYYSLKRPYEENIGNSVYKSFNILKKEFENESDIQ 883
[Close]
BLAST of SMED30030348 vs. Planmine SMEST
Match: SMESG000007934.1 (SMESG000007934.1)

HSP 1 Score: 1657.5 bits (4291), Expect = 0.000e+0
Identity = 882/882 (100.00%), Postives = 882/882 (100.00%), Query Frame = 3 
Query:   66 MLNSFNVEVKAAKTGIQNQCKIYNENYXXXXXXXXXXXXXXXDDETKAKCRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVLIGYSSKLVRTWRLTHENELPFLLKKHLTFLFXXXXXXXXXXXXXXXXLFEYCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIKIQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRYNNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLHFVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVEDYIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWLMLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEINSKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELLPKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVKLTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSWTVLIVLFGRCQXXXXXXXXXXXXYKDELDEKFSKVNKNMNVFKTCQNMIYYSLKRPYEENIGNSVYKSFNILKKEFENESDIQ 2711
            MLNSFNVEVKAAKTGIQNQCKIYNENYLLFKKFYEKLLLSKFDDETKAKCRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVLIGYSSKLVRTWRLTHENELPFLLKKHLTFLFEIENQINITAINTIIELFEYCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIKIQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRYNNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLHFVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVEDYIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWLMLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEINSKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELLPKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVKLTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSWTVLIVLFGRCQNLINGNILNNKNYKDELDEKFSKVNKNMNVFKTCQNMIYYSLKRPYEENIGNSVYKSFNILKKEFENESDIQ
Sbjct:    1 MLNSFNVEVKAAKTGIQNQCKIYNENYLLFKKFYEKLLLSKFDDETKAKCRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSEDENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVLIGYSSKLVRTWRLTHENELPFLLKKHLTFLFEIENQINITAINTIIELFEYCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIKIQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRYNNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLHFVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVEDYIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWLMLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEINSKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELLPKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVKLTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSWTVLIVLFGRCQNLINGNILNNKNYKDELDEKFSKVNKNMNVFKTCQNMIYYSLKRPYEENIGNSVYKSFNILKKEFENESDIQ 882
[Close]
BLAST of SMED30030348 vs. Planmine SMEST
Match: SMESG000056697.1 (SMESG000056697.1)

HSP 1 Score: 53.9138 bits (128), Expect = 5.906e-7
Identity = 49/186 (26.34%), Postives = 83/186 (44.62%), Query Frame = 3 
Query:  243 GNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNFTHIFYSVKPIMYSEMPNN---ILQYYEFDGKNVDNKLVGRTDQEPEIKQDLETIYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFDAGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTF 791
            GN  +   GQVK+GKS  +   L   V+  +     T   T + YS +   Y E+ +    +L+  + +   +  K +   D++   + ++  I  AG    F E    IID+PGL+E   +++     IK + P +++Y+ D       TD      L   +  KL+II+  +K D    TE  F
Sbjct:  103 GNPAIAIVGQVKAGKSTFVNVLLRKNVVCQAYE-RCTARLTRLTYSSQN--YVEILDENGKVLERKKLNKPTIPKKYLQLNDEDRTKRSEINKIVRAGIKSQFLEYGIDIIDSPGLSENATLTNLSKNMIKSILP-IMIYVIDGRTSVKQTDIADLLEL-KNAVPKLQIIYFISKLDTDDTTETEF 283
The following BLAST results are available for this feature:
BLAST of SMED30030348 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
A0A1I8HCN52.532e-727.51Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FEQ65.394e-727.51Protein kinase domain-containing protein OS=Macros... [more]
A0A1I8HTW66.515e-727.51Protein kinase domain-containing protein OS=Macros... [more]
A0A1I8FT075.130e-626.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30030348 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO498168.055e-623.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30030348 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030348 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000007934.10.000e+0100.00SMESG000007934.1[more]
SMESG000007934.10.000e+0100.00SMESG000007934.1[more]
SMESG000056697.15.906e-726.34SMESG000056697.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030348 ID=SMED30030348|Name=SMED30030348|organism=Schmidtea mediterranea sexual|type=transcript|length=2778bp
CGGAAATTAAAGATCAATATTGAACTTTGAAAGATGATAAATTTTATCAT
TTACAGCCGCGCATGATGCTAAATAGTTTCAATGTAGAAGTCAAAGCTGC
TAAAACTGGGATTCAAAATCAATGTAAAATTTATAATGAAAATTATTTAT
TATTTAAAAAATTTTATGAAAAATTGCTTTTATCAAAATTTGATGATGAG
ACCAAAGCAAAATGTAGATGCTCTTACAATGATCTCAATGAAGGAAATAT
TCGATTGGTATTCTGTGGTCAAGTAAAATCAGGCAAATCTCATTGTATCA
TTGAATTTCTTTCCGGAAAGGTTATCTTGCCTAGTCGCAGTATTCCAGCA
ACGATGAATTTTACTCACATTTTCTATTCAGTAAAACCAATTATGTATTC
GGAAATGCCAAACAATATACTTCAATACTATGAATTCGATGGGAAAAATG
TTGACAATAAATTAGTTGGTCGAACTGATCAGGAACCAGAAATTAAACAG
GATTTGGAAACGATTTATCATGCTGGAACACCATACACATTCGAAGAGAA
AAGTTTTCACATTATCGACACTCCAGGCTTAAATGAAACAAAAGAAATTT
CTGACGCGTTTTATATAAATATTAAAAAATTAAATCCACAATTAGTTGTG
TACATTTTTGACGCTGGACCTAAATTCACTGTCACAGATGAAGGGTTTTG
TACGAACCTAATTTCTCAGTCAAGACAAAAGTTGAAAATAATCTTTTTAT
TCAACAAATACGACCGTGCATTTGAAACAGAGAAAACCTTTCAAGGAAAT
TCAAGTGAGGATGAAAACACTGTTGAAAGTGATGTTTCAGACGATGACAA
TTTTGATACACCTGATGATGAGAAAAACATAAAAACCTATGACAAAGACT
ACAAAGATATCTATGAAGGTGTCAGTAAAAAATTCGACTATGACGATAGG
AAAATAGTTTTGATCGGCTATTCGAGCAAACTAGTTAGAACTTGGAGATT
AACGCACGAAAATGAGCTGCCTTTTTTATTGAAAAAACATTTGACTTTTC
TATTTGAAATAGAAAATCAAATTAATATAACTGCCATTAATACAATTATA
GAATTGTTTGAATACTGCAAATTGTATTTGTATTCCAAATTTAATATAGT
TTATTCCGATATTATTTCGACGAGGAAAAAATATTTTCACAGTTATACCC
TTAAAAGCACAAGAAAAGACAAACTGTTAAAGATGTACGACAAGTCTCAC
AGTGAAATTAAAATACAACCAATAGATTCAAACAAATTCGAATTAGATAG
TAGTATAAATGATATAATAAAAAGTCAAGAGAAGCGTTTGACAAATGAGT
CGATTGAAAATAAATATGAAAAAATCACGAAATCCTTAATTGGTTTTCTC
GTGAATCGTTATAACAATCTATTTTCTAATATTATTCATGTCAATCTCAA
TTTGGTTTCTTTCGATCTTCTTTGCAATGAAATCAAAGCCAACAATTTGG
AAAGCTATTTTTCTCACGCGTTTTGCACCTTGAAATATGATTATGATATT
TTCAATCTTCATTTTGTGATTGAACAACACTTTAATGATCAATACGCTAA
CCACTTTGAAGGAAAGGTTTCCAATGGATTCCAATCTATCGAAAACTATA
AGGAATACTTGAAAGAGAATTTGAAATATGACATTAAATATATTCTGCCG
AAAGTCGAAGATTATATTGGAATTAAAATAGAAAATGAAAGAAAGCATTT
GGTTCAGGCAATTGAAAGCAAAACCAATGAAATTATTTCTGCAGTGGAGA
AAACGGATCAAGAAACTGAATCAGAAATAAAAGATATAATTGAGGATTTT
AGAAAGTGGTTGATGTTAAATAATAACTACGTTAAATTATTAAAAAACAG
TTCTCGAAGAGAAAAATTTGCGAAAATCGTCGACTGTTGTGAGAATGGAG
AAGAAATTTCCACAAATAATCCAATTATCAAACGCGAGCATTTAGATTTA
AAGGAAATTAATAGTAAAAATTATGTCATCATTCAATACTTTTTGCGAAC
ACAAATCATTAGCAATTTTTTATTGGAAAATTTCATATATTTCCACGAAG
AGAGAAAAGAAATTTTTTACATTACAAATCTAGAAGATTTTCAACCGTTA
CGAGAACTTCTACCGAAACTGAACAGCGACAATAAGAGAATCCTTATTGG
TCAATTATCACAAATCGTTAGCACATTGTATGAATCATCTCTGCCATTGG
ATAATTTTGGGCCCGACATGGTATTTGGGAGATTATGCCCGAAACTTGGG
GTTGAGGTTAAACTGACGGAAACCAGCCAGAACTTCCGCCAGTGGCGATA
TACATTGGAAGATGGCCAAAAAAGCGATGAGGTATTTTTCAAATATCCTT
TTTATTTAGAAATGAAATCAATAGATGAAATGAATTTGAGCATGATTTAT
TCTTTCAGCTGGACTGTGCTTATTGTCTTGTTCGGCAGATGTCAAAATCT
TATCAATGGAAATATTTTAAACAATAAAAATTACAAAGATGAACTAGATG
AAAAATTCTCGAAAGTCAACAAGAATATGAACGTCTTTAAAACTTGCCAA
AATATGATTTATTATAGTTTAAAAAGACCGTATGAAGAAAACATTGGAAA
TAGTGTTTATAAGTCATTTAATATTTTGAAAAAGGAATTTGAAAATGAAT
CAGATATACAATGAAATATTTCTTTCCTATTTATGCATGCACTAATTTTG
CGACATTGTTTCATTTTTATAAATTGCC
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protein sequence of SMED30030348-orf-1

>SMED30030348-orf-1 ID=SMED30030348-orf-1|Name=SMED30030348-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=884bp
MMLNSFNVEVKAAKTGIQNQCKIYNENYLLFKKFYEKLLLSKFDDETKAK
CRCSYNDLNEGNIRLVFCGQVKSGKSHCIIEFLSGKVILPSRSIPATMNF
THIFYSVKPIMYSEMPNNILQYYEFDGKNVDNKLVGRTDQEPEIKQDLET
IYHAGTPYTFEEKSFHIIDTPGLNETKEISDAFYINIKKLNPQLVVYIFD
AGPKFTVTDEGFCTNLISQSRQKLKIIFLFNKYDRAFETEKTFQGNSSED
ENTVESDVSDDDNFDTPDDEKNIKTYDKDYKDIYEGVSKKFDYDDRKIVL
IGYSSKLVRTWRLTHENELPFLLKKHLTFLFEIENQINITAINTIIELFE
YCKLYLYSKFNIVYSDIISTRKKYFHSYTLKSTRKDKLLKMYDKSHSEIK
IQPIDSNKFELDSSINDIIKSQEKRLTNESIENKYEKITKSLIGFLVNRY
NNLFSNIIHVNLNLVSFDLLCNEIKANNLESYFSHAFCTLKYDYDIFNLH
FVIEQHFNDQYANHFEGKVSNGFQSIENYKEYLKENLKYDIKYILPKVED
YIGIKIENERKHLVQAIESKTNEIISAVEKTDQETESEIKDIIEDFRKWL
MLNNNYVKLLKNSSRREKFAKIVDCCENGEEISTNNPIIKREHLDLKEIN
SKNYVIIQYFLRTQIISNFLLENFIYFHEERKEIFYITNLEDFQPLRELL
PKLNSDNKRILIGQLSQIVSTLYESSLPLDNFGPDMVFGRLCPKLGVEVK
LTETSQNFRQWRYTLEDGQKSDEVFFKYPFYLEMKSIDEMNLSMIYSFSW
TVLIVLFGRCQNLINGNILNNKNYKDELDEKFSKVNKNMNVFKTCQNMIY
YSLKRPYEENIGNSVYKSFNILKKEFENESDIQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR022812Dynamin_SF
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 65..289
e-value: 1.0E-14
score: 56.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..264
IPR022812Dynamin superfamilyPFAMPF00350Dynamin_Ncoord: 65..229
e-value: 2.6E-10
score: 40.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 59..242