C-type lectin domain-containing protein

Overview
NameC-type lectin domain-containing protein
Smed IDSMED30030333
Length (bp)834
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of C-type lectin domain-containing protein (SMED30030333) t-SNE clustered cells

Violin plots show distribution of expression levels for C-type lectin domain-containing protein (SMED30030333) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of C-type lectin domain-containing protein (SMED30030333) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for C-type lectin domain-containing protein (SMED30030333) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030333

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30030333SMESG000058775.1 SMESG000058770.1 SMESG000058758.1 SmedASXL_071289SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30030333SMESG000058775.1 SMESG000058770.1 SMESG000058758.1 dd_Smed_v4_32483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030333SMESG000058775.1 SMESG000058770.1 SMESG000058758.1 dd_Smed_v4_32483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30030333SMESG000058775.1 SMESG000058770.1 SMESG000058758.1 dd_Smed_v4_32483_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030333SMESG000058775.1 Contig25009newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030333SMESG000058775.1 Contig25009uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30030333SMESG000058775.1 SMESG000058770.1 SMESG000058758.1 dd_Smed_v6_32483_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of C-type lectin domain-containing protein vs. TrEMBL
Match: A0A1I8HCD6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.085e-11
Identity = 58/213 (27.23%), Postives = 98/213 (46.01%), Query Frame = 1 
Query:  187 TYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTS--YWYNQEPNSMPSGEDCVVINT----KLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVS---------ARSVLECGAISLTKPYYR--SFYFDKIQSDCVLVKYVDSTLPLS---FNTSDR---QWIGF 756
            T+C+A   C + G  + S+S   A++         +++GL D L ER   + GW++    Q+ D S   + + EPN++ + +DCV I +    KL DT C    N FC    D  +  R     +T        E+  YD  S         A++ L C    L   +     +Y+  ++  CVL+ Y D++LPLS   F+ +++   QW+ +
Sbjct:  546 TFCDAIKTCHENGQEMISKSVHDAIV-ATSFSGDRWWVGLVDALNERGTQKDGWVWIKDGQVTDGSGSVFLSDEPNNLGNNQDCVTIKSDSIKKLRDTTCNDLNNVFCY--EDAAVANRIATKATTGKFVRT--EIVAYDQPSPGCWIKEPRAKTHLHCSQACLVFEFEDCIGYYWHPVRRQCVLLPYFDASLPLSGSDFSANNQEKLQWMKY 753
[Close]
BLAST of C-type lectin domain-containing protein vs. TrEMBL
Match: A0A1I8J6T3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.572e-10
Identity = 58/198 (29.29%), Postives = 95/198 (47.98%), Query Frame = 1 
Query:  187 TYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQ---LNDTSYWYNQEPNSMPSGEDCVVI-NTKLYDTRCKGKFNYFC--IANSDDEIHTR--FLKSNSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 756
            ++C+A   C  IGG L    D++A+   N   + ++YIG+ DLL E   +++GW F++GS    LNDT  W N  PN+    +D +V+ N  L D        + C  IA  + E   R  FL+  +  P++       CY   S  S   C  + + +   R+  F++  S C ++ + DS++  +  T D  W  F
Sbjct:  484 SWCDAARLCLGIGGELLI--DLTAIRAVNHWLD-YYYIGVTDLLVEAKWSRSGWRFANGSLFPVLNDTE-WMNHSPNNNSPDDDFLVVSNAGLRDQPANSATKFVCQPIATPNSEYLRRAEFLRLGNISPVY-------CYTDASVSSEFHCLWLCVQRWECRAVLFNRASSACRMLDFADSSVGDAIET-DPSWTKF 669
[Close]
BLAST of C-type lectin domain-containing protein vs. TrEMBL
Match: A0A1I8HX01 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.632e-10
Identity = 58/198 (29.29%), Postives = 95/198 (47.98%), Query Frame = 1 
Query:  187 TYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQ---LNDTSYWYNQEPNSMPSGEDCVVI-NTKLYDTRCKGKFNYFC--IANSDDEIHTR--FLKSNSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 756
            ++C+A   C  IGG L    D++A+   N   + ++YIG+ DLL E   +++GW F++GS    LNDT  W N  PN+    +D +V+ N  L D        + C  IA  + E   R  FL+  +  P++       CY   S  S   C  + + +   R+  F++  S C ++ + DS++  +  T D  W  F
Sbjct:  484 SWCDAARLCLGIGGELLI--DLTAIRAVNHWLD-YYYIGVTDLLVEAKWSRSGWRFANGSLFPVLNDTE-WMNHSPNNNSPDDDFLVVSNAGLRDQPANSATKFVCQPIATPNSEYLRRAEFLRLGNISPVY-------CYTDASVSSEFHCLWLCVQRWECRAVLFNRASSACRMLDFADSSVGDAIET-DPSWTKF 669
[Close]
BLAST of C-type lectin domain-containing protein vs. TrEMBL
Match: A0A1I8I381 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.713e-10
Identity = 54/211 (25.59%), Postives = 95/211 (45.02%), Query Frame = 1 
Query:  187 TYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTS--YWYNQEPNSMPSGEDCVVINT----KLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDV------EVGCY-DIVSARSVLECGAISLTKPYYR--SFYFDKIQSDCVLVKYVDSTLPLS---FNTSDR---QWIGF 756
            T+C A   C + G  + S+S +   M         +++GL D L ER   + GW++    Q+ D S   + + EPN++ + +DC  I +    KL D  C    N FC    D  +  R     +T      ++      + GC+     A++ L C    L   +     +Y+  ++  CVL+ Y D++LPLS   F+ +++   QW+ +
Sbjct:  178 TFCEAIKTCHENGQEMISKS-VHDAMAATSFSGDRWWVGLVDALNERDTQEDGWVWIKDGQVTDGSGSVFLSGEPNNLGNNQDCATIKSDSIRKLRDETCNELNNVFCY--EDAAVANRIATKATTGKFVRTEIVAYDQPDPGCWIKEPRAKTHLRCSQACLAFEFEDCIGYYWHPVRRQCVLLPYFDASLPLSGSDFSANNQEKLQWMKY 385

HSP 2 Score: 70.4774 bits (171), Expect = 8.860e-10
Identity = 62/253 (24.51%), Postives = 104/253 (41.11%), Query Frame = 1 
Query:  115 IFLSYSVTYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLND--TSYWYNQEPNSMPSGEDCVVI----NTKLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVS---------ARSVLECGAISLTKPYYR--SFYFDKIQSDCVLVKYVDSTLPLS------FNTSDRQWIGFN*EVFVSSLSFLENSH 804
            +F  + V ++ C  L            + +  C + G  + S+S +   M         +++GL D L ER   + GW++    Q+ D   SY+Y+ EPN+  + +DCV +    + KL D  C    N FC    D  +  R     +T        E+  YD  S         A++ L C    L   +     +Y+  ++  CVL+ Y D++LPLS       N    QW+ +   +  SS +   + H
Sbjct: 1032 VFYDFRVDFESCPVLM----------SDYYFTCHENGQEMISKS-VHDAMAATSFSGDRWWVGLIDALNERGTQKDGWVWIKDGQVTDGGESYFYSGEPNNEENNQDCVTVRKNSDKKLRDETCNELNNVFCY--EDAAVANRIATKATTGKFVRT--EIVAYDQPSPGCWIKGPRAKTHLHCSQACLVFEFEDCIGYYWHPVRRQCVLLPYFDASLPLSGSDFSANNEEKLQWMKYK-RIHASSATHRPDHH 1268
[Close]
BLAST of C-type lectin domain-containing protein vs. TrEMBL
Match: A0A1I8J9T8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.042e-9
Identity = 58/203 (28.57%), Postives = 87/203 (42.86%), Query Frame = 1 
Query:  190 YCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLF---SDGSQLNDTSYWYNQEPNSMPSGEDCVVINT---KLYDTRCKGKFNYFCIANSDDEI-----HTRFLKSNSTRPIFN-NDVEVGCYDIVSARSVLE--CGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQ--WI 750
            YC     C  +G R+      +AV     L     + G+ND L ER  + TGW       GS L  T  W   +P+   S EDCV  N    KL+D  C       C     DE      +  F++S    P+ +     VGC + ++    +E  C      K     FYF+ ++ +CVL+ Y+D+ + LS+++   Q  WI
Sbjct:   44 YCQCLKTCAQLGQRILQPDTYAAVR----LSLGEVWTGINDQLVERGSSPTGWQVVPNRAGSLLTLTDLWNTGQPDGT-SIEDCVNHNPTTKKLHDAGCTATIACRCEHPMGDERPVPAHYELFVES----PVVDFTQGAVGCTEKIALELPIELLCAGECREKLGCVGFYFNPLRRECVLLLYMDAAVSLSYSSPAEQAAWI 237
[Close]
BLAST of C-type lectin domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000053475.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001786.1:256441:258282:1 gene:ENSAMXG00000030239.1 transcript:ENSAMXT00000053475.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.9802 bits (110), Expect = 3.238e-6
Identity = 47/170 (27.65%), Postives = 70/170 (41.18%), Query Frame = 1 
Query:    1 VKQNL**NRKSFVRNNLIVIGLFSRA*MTFGVIIIFINIFLSYSVTYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLA----SESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTKLY--DTRCKGKFNYFC 492
            +K+ L   +KSF R   IV G FS          +F+N+   + V+               +T       C   GG+LA     E +           +A+ +IG ND +     N+  ++ ++G  L D   W   EPNS    EDC+VINT  +  DT C+  F   C
Sbjct:   15 IKKRLLELQKSFTRFTEIVTGRFS----------VFVNVGHKFFVS-----------DGETKTISEGGRICQKAGGKLALPRNEEENQKLSGALRAFGSAYMFIGANDQV-----NEGQFVDTEGRNL-DYLKWNTGEPNSYGGNEDCIVINTNGFWLDTSCEKSFLIAC 157
[Close]
BLAST of C-type lectin domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005222.1 (pep scaffold:Pmarinus_7.0:GL492592:1201:2726:-1 gene:ENSPMAG00000004753.1 transcript:ENSPMAT00000005222.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.8246 bits (107), Expect = 1.240e-6
Identity = 34/109 (31.19%), Postives = 53/109 (48.62%), Query Frame = 1 
Query:  175 TTQETYCNAWSKCFDIGGRLA-SESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVI--NTKLYDTRCKGKFNYFC 492
            T    Y +A  +C + GG LA  ++ I       ++     +IGLND+     +++  ++FSDGS+L     +   EPNS    EDCV I  N K  D  C  + ++ C
Sbjct:   31 TNPSNYHSARQQCANHGGVLAMPKNAIQNAGIQQVVRGDRAFIGLNDI-----QHENNFVFSDGSRLGLYRNFALGEPNSFGGDEDCVNIYPNGKWNDIGCHTQLHFIC 134
[Close]
BLAST of C-type lectin domain-containing protein vs. Planmine SMEST
Match: SMESG000058775.1 (SMESG000058775.1)

HSP 1 Score: 447.973 bits (1151), Expect = 1.757e-161
Identity = 214/225 (95.11%), Postives = 216/225 (96.00%), Query Frame = 1 
Query:   82 MTFGVIIIFINIFLSYSVTYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTKLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 756
            MTFG +IIFINIFLSYS+TYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDIS VMDCNMLPN HFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTK YDT CKG FNYFCIANS DEIH RFLKSNS RPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF
Sbjct:    1 MTFGALIIFINIFLSYSITYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDISTVMDCNMLPNVHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTKFYDTSCKGNFNYFCIANSYDEIHARFLKSNSIRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 225
[Close]
BLAST of C-type lectin domain-containing protein vs. Planmine SMEST
Match: SMESG000058770.1 (SMESG000058770.1)

HSP 1 Score: 418.313 bits (1074), Expect = 1.607e-149
Identity = 207/240 (86.25%), Postives = 212/240 (88.33%), Query Frame = 1 
Query:   82 MTFGVIIIFINIFLSYSVTYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTKLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVSARSVLECGA---------------ISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 756
            MTFG +IIFINIFLSYS+TYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDIS VMDCNMLPN HFYIGLNDLLFERYKNQTGWLFSDGSQLN+TSYW   EPNS+ SGEDCVVI TKLYDT CKG FNYFCIANS DEIHTR LKSNSTRPIFNNDVEVGCYDIVSA+SVLECGA                SLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF
Sbjct:    1 MTFGALIIFINIFLSYSITYKPCQQLQVYISTTQETYCNAWSKCFDIGGRLASESDISTVMDCNMLPNVHFYIGLNDLLFERYKNQTGWLFSDGSQLNNTSYWNGNEPNSLYSGEDCVVIKTKLYDTSCKGNFNYFCIANSYDEIHTRILKSNSTRPIFNNDVEVGCYDIVSAKSVLECGARYDCCIGIFLCFVTCRSLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 240
[Close]
BLAST of C-type lectin domain-containing protein vs. Planmine SMEST
Match: SMESG000058758.1 (SMESG000058758.1)

HSP 1 Score: 386.341 bits (991), Expect = 2.604e-128
Identity = 184/201 (91.54%), Postives = 188/201 (93.53%), Query Frame = 1 
Query:  154 QLQVYISTTQETYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTKLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 756
            +LQVYISTTQETYCNAWSKCFDIGGRLASESDIS VMDCN+LPN HFYIGLNDLLFERYKNQTGWLFSDGSQLN+TSYW  QEPNSM   EDCVVINTKLYDT CKG FNYFCIANS DEIH R LK NSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIG+
Sbjct:  697 KLQVYISTTQETYCNAWSKCFDIGGRLASESDISTVMDCNILPNVHFYIGLNDLLFERYKNQTGWLFSDGSQLNNTSYWNTQEPNSMDCVEDCVVINTKLYDTSCKGNFNYFCIANSYDEIHARNLKRNSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGY 897
[Close]
BLAST of C-type lectin domain-containing protein vs. Planmine SMEST
Match: SMESG000058758.1 (SMESG000058758.1)

HSP 1 Score: 297.36 bits (760), Expect = 1.434e-94
Identity = 141/158 (89.24%), Postives = 145/158 (91.77%), Query Frame = 1 
Query:  154 QLQVYISTTQETYCNAWSKCFDIGGRLASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVINTKLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVSARSVLECGAI 627
            +LQVYISTTQETYCNAWSKCFDIGGRLASESDIS VMDCN+LPN HFYIGLNDLLFERYKNQTGWLFSDGSQLN+TSYW  QEPNSM   EDCVVINTKLYDT CKG FNYFCIANS DEIH R LK NSTRPIFNNDVEVGCYDIVSARSVLECGA+
Sbjct:  697 KLQVYISTTQETYCNAWSKCFDIGGRLASESDISTVMDCNILPNVHFYIGLNDLLFERYKNQTGWLFSDGSQLNNTSYWNTQEPNSMDCVEDCVVINTKLYDTSCKGNFNYFCIANSYDEIHARNLKRNSTRPIFNNDVEVGCYDIVSARSVLECGAM 854
[Close]
BLAST of C-type lectin domain-containing protein vs. Planmine SMEST
Match: SMESG000058753.1 (SMESG000058753.1)

HSP 1 Score: 179.874 bits (455), Expect = 4.261e-56
Identity = 88/210 (41.90%), Postives = 131/210 (62.38%), Query Frame = 1 
Query:  184 ETYCNAWSKCFDIGGRLASESDISAVMDCNMLPN-AHFYIGLNDLLFERYKNQTGWLFSDGSQLNDTSYWYNQEPNSMPSGEDCVVI-NTKLYDTRCKGKFNYFCIANSDDEIHTRFLKSNSTRPIFNNDVEVGCYDIVSARSVLECGAI-----------------SLTKPYYRSFYFDKIQSDCVLVKYVDSTLPLSFNTSDRQWIGF 756
            + YC+AW+ C++ GGRL  E+DI  +  C ++ +  +++IGLNDL+ ER+ N++GW+FSDGS+L +T YW + +PNS+ +GEDCVVI N  ++D  C+  + Y C+ + + +   R   SN++RPI+NND++ GCYD   A + L+C  +                 SL+   Y+SFYFD    DCV  KYVDSTL   FN  DR+W  F
Sbjct:    2 KNYCDAWNTCYNSGGRLILENDIPIIKSCGVISSYNNYWIGLNDLVNERFTNKSGWIFSDGSELMNTGYWDSSQPNSLRAGEDCVVIQNNAIFDIECEETYKYVCVKSEEFKFRFRNFVSNASRPIYNNDIQSGCYDAYKAETTLQCAMLYELVTYYIFFIAILFHRSLSHSNYKSFYFDSESKDCVWSKYVDSTLSAQFNQFDRKWRIF 211
The following BLAST results are available for this feature:
BLAST of C-type lectin domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HCD64.085e-1127.23Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8J6T31.572e-1029.29Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HX011.632e-1029.29Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I3816.713e-1025.59Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8J9T85.042e-928.57Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of C-type lectin domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000053475.13.238e-627.65pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of C-type lectin domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005222.11.240e-631.19pep scaffold:Pmarinus_7.0:GL492592:1201:2726:-1 ge... [more]
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BLAST of C-type lectin domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of C-type lectin domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000058775.11.757e-16195.11SMESG000058775.1[more]
SMESG000058770.11.607e-14986.25SMESG000058770.1[more]
SMESG000058758.12.604e-12891.54SMESG000058758.1[more]
SMESG000058758.11.434e-9489.24SMESG000058758.1[more]
SMESG000058753.14.261e-5641.90SMESG000058753.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030333 ID=SMED30030333|Name=C-type lectin domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=834bp
GTAAAACAAAATTTATAATGAAATAGAAAGTCTTTTGTCAGGAACAATTT
AATCGTTATTGGTTTATTTTCACGAGCTTAAATGACATTTGGTGTCATTA
TTATTTTTATTAACATTTTCCTCTCTTATTCTGTAACTTATAAACCTTGT
CAACAGCTCCAGGTTTACATTTCGACGACTCAAGAAACTTATTGTAATGC
TTGGTCAAAATGCTTCGACATTGGCGGGAGGCTGGCAAGCGAAAGTGACA
TCTCGGCAGTGATGGATTGCAACATGTTACCGAATGCTCATTTTTATATT
GGGCTGAATGACCTCCTATTTGAAAGGTACAAAAACCAGACAGGATGGCT
GTTCAGTGATGGATCACAGTTGAATGATACAAGTTATTGGTATAACCAAG
AGCCTAATTCGATGCCTAGTGGAGAAGATTGCGTTGTCATAAATACCAAA
TTATATGACACCCGTTGCAAAGGTAAATTCAACTATTTTTGCATAGCTAA
TAGCGACGATGAAATACACACAAGATTTCTCAAAAGTAACTCGACTCGCC
CTATTTTTAACAACGATGTCGAAGTCGGATGTTACGATATTGTCTCTGCG
AGAAGTGTTCTGGAATGCGGGGCAATATCATTGACCAAGCCCTATTATAG
ATCATTTTATTTCGATAAAATTCAAAGCGATTGCGTGCTCGTGAAATATG
TGGACAGCACTTTGCCTCTCAGCTTCAACACGTCTGACCGCCAGTGGATC
GGATTTAATTAAGAAGTTTTTGTTTCCTCGCTTTCTTTCCTAGAAAATTC
ACATTGCACTTTTCTGGTTAAACTTATCCAATTT
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protein sequence of SMED30030333-orf-1

>SMED30030333-orf-1 ID=SMED30030333-orf-1|Name=SMED30030333-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=227bp
MTFGVIIIFINIFLSYSVTYKPCQQLQVYISTTQETYCNAWSKCFDIGGR
LASESDISAVMDCNMLPNAHFYIGLNDLLFERYKNQTGWLFSDGSQLNDT
SYWYNQEPNSMPSGEDCVVINTKLYDTRCKGKFNYFCIANSDDEIHTRFL
KSNSTRPIFNNDVEVGCYDIVSARSVLECGAISLTKPYYRSFYFDKIQSD
CVLVKYVDSTLPLSFNTSDRQWIGFN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR001304C-type_lectin-like
IPR016186C-type_lectin-like/link_sf
IPR016187CTDL_fold
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001304C-type lectin-likeSMARTSM00034CLECT_2coord: 14..138
e-value: 2.1E-4
score: 30.6
IPR001304C-type lectin-likePFAMPF00059Lectin_Ccoord: 34..137
e-value: 1.0E-7
score: 32.6
IPR001304C-type lectin-likePROSITEPS50041C_TYPE_LECTIN_2coord: 29..138
score: 11.124
IPR016186C-type lectin-like/link domain superfamilyGENE3DG3DSA:3.10.100.10coord: 13..139
e-value: 3.3E-15
score: 58.0
NoneNo IPR availablePANTHERPTHR22802C-TYPE LECTIN SUPERFAMILY MEMBERcoord: 25..137
NoneNo IPR availablePANTHERPTHR22802:SF385coord: 25..137
NoneNo IPR availableCDDcd00037CLECTcoord: 29..137
e-value: 6.02709E-10
score: 53.0074
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.682
IPR016187C-type lectin foldSUPERFAMILYSSF56436C-type lectin-likecoord: 28..140