RNA pseudouridine synthase D4

Overview
NameRNA pseudouridine synthase D4
Smed IDSMED30030321
Length (bp)1268
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of RNA pseudouridine synthase D4 (SMED30030321) t-SNE clustered cells

Violin plots show distribution of expression levels for RNA pseudouridine synthase D4 (SMED30030321) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RNA pseudouridine synthase D4 (SMED30030321) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RNA pseudouridine synthase D4 (SMED30030321) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030321

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30030321SMESG000060928.1 SmedASXL_010344SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
reproductive organSMED30030321SMESG000060928.1 Contig7343newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30030321SMESG000060928.1 Contig7343uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Human
Match: RPUSD4 (RNA pseudouridine synthase D4 [Source:HGNC Symbol;Acc:HGNC:25898])

HSP 1 Score: 140.969 bits (354), Expect = 2.646e-37
Identity = 98/308 (31.82%), Postives = 158/308 (51.30%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXX----XXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQ-----IVIRPDMKSVKKNIKKAGFTKSESGKIS-TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
              +++  + ++  L R  L  + +++ INKP+G+ V GG  G  +     L            E LH+ HRLDK+TTGV++LA + D A  +++ F+ R + K Y  +T  +P    G+++  + E     Q     + + P  +    + K     +S + +++ T + VL S  + +LV+   I+G+KHQ+R HL++GL+ PILGDHKYS W    N+  + PQ+LS   L  L L Q K R++P+HLHA QLI+       +L  GK  LN               +   +P FF+ SL  L+L 
Sbjct:   82 QLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGP-GVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRM--DDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDW----NR--LAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLIL------PALGSGKEELN---------------LVCKLPRFFVHSLHRLRLE 359
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Human
Match: RPUSD4 (RNA pseudouridine synthase D4 [Source:HGNC Symbol;Acc:HGNC:25898])

HSP 1 Score: 135.191 bits (339), Expect = 2.234e-35
Identity = 95/302 (31.46%), Postives = 147/302 (48.68%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXX----XXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
              +++  + ++  L R  L  + +++ INKP+G+ V GG  G  +     L            E LH+ HRLDK+TTGV++LA + D A  +++ F+ R + K Y  +T  +P    G+++  + E                            E+    T + VL S  + +LV+   I+G+KHQ+R HL++GL+ PILGDHKYS W    N+  + PQ+LS   L  L L Q K R++P+HLHA QLI+       +L  GK  LN               +   +P FF+ SL  L+L 
Sbjct:   82 QLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGP-GVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVE---------------------------KEAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDW----NR--LAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLIL------PALGSGKEELN---------------LVCKLPRFFVHSLHRLRLE 328
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Human
Match: RPUSD2 (RNA pseudouridine synthase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:24180])

HSP 1 Score: 61.6178 bits (148), Expect = 1.501e-9
Identity = 51/184 (27.72%), Postives = 80/184 (43.48%), Query Frame = 1 
Query:  376 FLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
             L  N  V+ ++KP  I V     G      +  +  K    + LH +HRLD+ T+GVL+ A+    +  + +  ++R + K Y+C   G          F  +E T  + I++     S K  + +            T F  L  N   S+V+C  ++G  HQIR HL + L  PIL D  Y
Sbjct:  225 LLAENEDVVVVDKPSSIPVH--PCGRFRHNTVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSERIHEQVRDRQLEKEYVCRVEG---------EFPTEEVTCKEPILV----VSYKVGVCR---VDPRGKPCETVFQRLSYNGQSSVVRCRPLTGRTHQIRVHLQF-LGHPILNDPIY 389
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Human
Match: RPUSD2 (RNA pseudouridine synthase domain containing 2 [Source:HGNC Symbol;Acc:HGNC:24180])

HSP 1 Score: 61.2326 bits (147), Expect = 1.681e-9
Identity = 51/184 (27.72%), Postives = 80/184 (43.48%), Query Frame = 1 
Query:  376 FLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
             L  N  V+ ++KP  I V     G      +  +  K    + LH +HRLD+ T+GVL+ A+    +  + +  ++R + K Y+C   G          F  +E T  + I++     S K  + +            T F  L  N   S+V+C  ++G  HQIR HL + L  PIL D  Y
Sbjct:  164 LLAENEDVVVVDKPSSIPVH--PCGRFRHNTVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSERIHEQVRDRQLEKEYVCRVEG---------EFPTEEVTCKEPILV----VSYKVGVCR---VDPRGKPCETVFQRLSYNGQSSVVRCRPLTGRTHQIRVHLQF-LGHPILNDPIY 328
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Human
Match: RPUSD3 (RNA pseudouridine synthase D3 [Source:HGNC Symbol;Acc:HGNC:28437])

HSP 1 Score: 50.0618 bits (118), Expect = 5.012e-6
Identity = 70/260 (26.92%), Postives = 112/260 (43.08%), Query Frame = 1 
Query:  331 KKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASI-XXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRK------NYLCLTHGIPHEEHGIINFNLK-EATLGDQIVI---RPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-----------WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLH 1044
            K LS++E+   L    +     ++ +NKP G+ V G     ++   + EL  +    ++ L +V    K+++G++LL+  P +A  L+  F   H R+       Y  +T GIP    G I   LK E   G  + +    P  K + + +KK            + F V+ +    +LVQ   ++    Q++ H+   L  P+LGDH YS            L  EN  P   Q L    L  L LT  +   LP+HLH
Sbjct:   68 KNLSREELVDALRAAVVDRKGPLVTLNKPQGLPVTGKPGELTLFSVLPELSQSLGLREQELQVVRASGKESSGLVLLSSCPQTASRLQKYFT--HARRAQRPTATYCAVTDGIPAASEGKIQAALKLEHIDGVNLTVPVKAPSRKDILEGVKKT----------LSHFRVVATGSGCALVQLQPLTVFSSQLQVHMVLQL-CPVLGDHMYSARVGTVLGQRFLLPAENNKPQR-QVLDEALLRRLHLTPSQAAQLPLHLH 313
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Celegans
Match: K07E8.7 (Pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:A0A0M9JJ73])

HSP 1 Score: 61.2326 bits (147), Expect = 5.129e-10
Identity = 45/145 (31.03%), Postives = 66/145 (45.52%), Query Frame = 1 
Query:  514 IVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGI-PHEEHGIINFNLKEATLGDQIV------IRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            ++HRLD+ T+GVLL A+N ++    K   K+    K Y+C   G+ P EE       + E  +G  ++      +RPD K  K   +K                 L S+   S+VQ  + +G  HQIR H  + L  PI GD  Y
Sbjct:  194 VLHRLDRATSGVLLFAKNYETDLEFKTTLKQGEWSKEYICKVDGVFPDEEQ------VCEQPIGPLVISMGIQCVRPDGKDAKSRFRK-----------------LWSDGTQSVVQVHIETGRTHQIRVHSQF-LGHPIAGDQIY 314
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Celegans
Match: K07E8.7 (Pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:A0A0M9JJ73])

HSP 1 Score: 61.2326 bits (147), Expect = 5.309e-10
Identity = 45/146 (30.82%), Postives = 67/146 (45.89%), Query Frame = 1 
Query:  514 IVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGI-PHEEHGIINFNLKEATLGDQIV------IRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            ++HRLD+ T+GVLL A+N ++    K   K+    K Y+C   G+ P EE       + E  +G  ++      +RPD K  K   +K                 L S+   S+VQ  + +G  HQIR H  + L  PI GD  Y+
Sbjct:  183 VLHRLDRATSGVLLFAKNYETDLEFKTTLKQGEWSKEYICKVDGVFPDEEQ------VCEQPIGPLVISMGIQCVRPDGKDAKSRFRK-----------------LWSDGTQSVVQVHIETGRTHQIRVHSQF-LGHPIAGDQIYN 304
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Celegans
Match: K07E8.7 (Pseudouridine synthase [Source:UniProtKB/TrEMBL;Acc:A0A0M9JJ73])

HSP 1 Score: 60.8474 bits (146), Expect = 5.524e-10
Identity = 45/146 (30.82%), Postives = 67/146 (45.89%), Query Frame = 1 
Query:  514 IVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGI-PHEEHGIINFNLKEATLGDQIV------IRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            ++HRLD+ T+GVLL A+N ++    K   K+    K Y+C   G+ P EE       + E  +G  ++      +RPD K  K   +K                 L S+   S+VQ  + +G  HQIR H  + L  PI GD  Y+
Sbjct:  141 VLHRLDRATSGVLLFAKNYETDLEFKTTLKQGEWSKEYICKVDGVFPDEE------QVCEQPIGPLVISMGIQCVRPDGKDAKSRFRK-----------------LWSDGTQSVVQVHIETGRTHQIRVHSQF-LGHPIAGDQIYN 262
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Fly
Match: RluA-1 (gene:FBgn0051719 transcript:FBtr0080040)

HSP 1 Score: 63.5438 bits (153), Expect = 2.046e-10
Identity = 46/138 (33.33%), Postives = 67/138 (48.55%), Query Frame = 1 
Query:  517 VHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGK-ISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            +HRLD+ T+G+LL  ++P  A  ++   + R + K Y+C   G+  +  GI+    KE           ++ S K  + K     S  GK  +T F  L  N   S+V C  ++G  HQIR HL Y L  PIL D  Y
Sbjct:  424 IHRLDRLTSGLLLFGRSPKKARQMEQQIRNRQVEKEYICRVEGVFPD--GIV--ECKEPI---------EVVSYKIGVCKV----SAKGKDCTTTFQKLSQNGTTSVVLCKPLTGRMHQIRVHLQY-LGYPILNDPLY 543
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Fly
Match: RluA-1 (gene:FBgn0051719 transcript:FBtr0080039)

HSP 1 Score: 63.5438 bits (153), Expect = 2.046e-10
Identity = 46/138 (33.33%), Postives = 67/138 (48.55%), Query Frame = 1 
Query:  517 VHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGK-ISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            +HRLD+ T+G+LL  ++P  A  ++   + R + K Y+C   G+  +  GI+    KE           ++ S K  + K     S  GK  +T F  L  N   S+V C  ++G  HQIR HL Y L  PIL D  Y
Sbjct:  424 IHRLDRLTSGLLLFGRSPKKARQMEQQIRNRQVEKEYICRVEGVFPD--GIV--ECKEPI---------EVVSYKIGVCKV----SAKGKDCTTTFQKLSQNGTTSVVLCKPLTGRMHQIRVHLQY-LGYPILNDPLY 543
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Fly
Match: RluA-1 (gene:FBgn0051719 transcript:FBtr0080041)

HSP 1 Score: 63.5438 bits (153), Expect = 2.046e-10
Identity = 46/138 (33.33%), Postives = 67/138 (48.55%), Query Frame = 1 
Query:  517 VHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGK-ISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            +HRLD+ T+G+LL  ++P  A  ++   + R + K Y+C   G+  +  GI+    KE           ++ S K  + K     S  GK  +T F  L  N   S+V C  ++G  HQIR HL Y L  PIL D  Y
Sbjct:  424 IHRLDRLTSGLLLFGRSPKKARQMEQQIRNRQVEKEYICRVEGVFPD--GIV--ECKEPI---------EVVSYKIGVCKV----SAKGKDCTTTFQKLSQNGTTSVVLCKPLTGRMHQIRVHLQY-LGYPILNDPLY 543
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Fly
Match: RluA-2 (gene:FBgn0032256 transcript:FBtr0304133)

HSP 1 Score: 54.299 bits (129), Expect = 1.504e-7
Identity = 42/141 (29.79%), Postives = 63/141 (44.68%), Query Frame = 1 
Query:  517 VHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHG-IPHEEHGIINFNLKEATLGDQI---VIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            +HRLD+ T+G+LL  +  + A  L+   + R ++K Y+C   G  P    GI+  N K   +  +I    + P  K  K   K+ G   S+                 S+V C  ++G  HQIR HL + L  PI  D  Y
Sbjct:  263 IHRLDRLTSGLLLFGRTAEKARELELQIRTRQVQKEYVCRVEGRFP---DGIVECNEKIDVVSYKIGVCKVSPKGKDCKTTFKRIGEVGSD-----------------SIVLCKPLTGRMHQIRVHLQF-LGYPISNDPLY 382
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Fly
Match: RluA-2 (gene:FBgn0032256 transcript:FBtr0337002)

HSP 1 Score: 53.9138 bits (128), Expect = 1.620e-7
Identity = 42/141 (29.79%), Postives = 63/141 (44.68%), Query Frame = 1 
Query:  517 VHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHG-IPHEEHGIINFNLKEATLGDQI---VIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            +HRLD+ T+G+LL  +  + A  L+   + R ++K Y+C   G  P    GI+  N K   +  +I    + P  K  K   K+ G   S+                 S+V C  ++G  HQIR HL + L  PI  D  Y
Sbjct:  263 IHRLDRLTSGLLLFGRTAEKARELELQIRTRQVQKEYVCRVEGRFP---DGIVECNEKIDVVSYKIGVCKVSPKGKDCKTTFKRIGEVGSD-----------------SIVLCKPLTGRMHQIRVHLQF-LGYPISNDPLY 382
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Zebrafish
Match: rpusd4 (RNA pseudouridine synthase D4 [Source:NCBI gene;Acc:445101])

HSP 1 Score: 111.694 bits (278), Expect = 5.190e-27
Identity = 79/243 (32.51%), Postives = 132/243 (54.32%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGI---SVQGGDNG-ASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKE-ATLGDQIVIRPDMKSV-KKNIKKAGFTKSESGKIS----TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII 1062
            L R  L  + ++IAINKP+ +   ++ G  N  A    +L  +   +    +LH+ H+L+K+TTGVL+LA+  ++A  ++   +   +   YL +T G+P    G+I+  + E A +G Q   +  +  + K N    G T+  + + +    T + VL +    SLV+   ++GVK+Q+R H+A  L  PILGDHKYS   K     + PQ+L    L  L L Q K R+LP+HLH+ ++++
Sbjct:   87 LYRSSLYEDQNIIAINKPYDVPLHNINGIRNSIAECLPLLAKITDNMRPGSQLHLCHKLEKETTGVLILAKTEEAAEHVQTLIQSHKVEMKYLAITVGVPVPSEGVIDIPVIERAVVGPQPHFKMALSPLFKVNEDGDGVTRVRAHRQAHAAVTRYQVLDNTSGCSLVELQPLTGVKNQLRVHMALALTCPILGDHKYSHWSK-----LAPQKLPEGTLRRLGLVQSKTRYLPLHLHSRRIVL 324
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Zebrafish
Match: rpusd4 (RNA pseudouridine synthase D4 [Source:NCBI gene;Acc:445101])

HSP 1 Score: 111.694 bits (278), Expect = 5.190e-27
Identity = 79/243 (32.51%), Postives = 132/243 (54.32%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGI---SVQGGDNG-ASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKE-ATLGDQIVIRPDMKSV-KKNIKKAGFTKSESGKIS----TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII 1062
            L R  L  + ++IAINKP+ +   ++ G  N  A    +L  +   +    +LH+ H+L+K+TTGVL+LA+  ++A  ++   +   +   YL +T G+P    G+I+  + E A +G Q   +  +  + K N    G T+  + + +    T + VL +    SLV+   ++GVK+Q+R H+A  L  PILGDHKYS   K     + PQ+L    L  L L Q K R+LP+HLH+ ++++
Sbjct:   87 LYRSSLYEDQNIIAINKPYDVPLHNINGIRNSIAECLPLLAKITDNMRPGSQLHLCHKLEKETTGVLILAKTEEAAEHVQTLIQSHKVEMKYLAITVGVPVPSEGVIDIPVIERAVVGPQPHFKMALSPLFKVNEDGDGVTRVRAHRQAHAAVTRYQVLDNTSGCSLVELQPLTGVKNQLRVHMALALTCPILGDHKYSHWSK-----LAPQKLPEGTLRRLGLVQSKTRYLPLHLHSRRIVL 324
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Zebrafish
Match: rpusd4 (RNA pseudouridine synthase D4 [Source:NCBI gene;Acc:445101])

HSP 1 Score: 111.694 bits (278), Expect = 5.190e-27
Identity = 79/243 (32.51%), Postives = 132/243 (54.32%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGI---SVQGGDNG-ASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKE-ATLGDQIVIRPDMKSV-KKNIKKAGFTKSESGKIS----TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII 1062
            L R  L  + ++IAINKP+ +   ++ G  N  A    +L  +   +    +LH+ H+L+K+TTGVL+LA+  ++A  ++   +   +   YL +T G+P    G+I+  + E A +G Q   +  +  + K N    G T+  + + +    T + VL +    SLV+   ++GVK+Q+R H+A  L  PILGDHKYS   K     + PQ+L    L  L L Q K R+LP+HLH+ ++++
Sbjct:   87 LYRSSLYEDQNIIAINKPYDVPLHNINGIRNSIAECLPLLAKITDNMRPGSQLHLCHKLEKETTGVLILAKTEEAAEHVQTLIQSHKVEMKYLAITVGVPVPSEGVIDIPVIERAVVGPQPHFKMALSPLFKVNEDGDGVTRVRAHRQAHAAVTRYQVLDNTSGCSLVELQPLTGVKNQLRVHMALALTCPILGDHKYSHWSK-----LAPQKLPEGTLRRLGLVQSKTRYLPLHLHSRRIVL 324
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Zebrafish
Match: rpusd4 (RNA pseudouridine synthase D4 [Source:NCBI gene;Acc:445101])

HSP 1 Score: 109.768 bits (273), Expect = 2.280e-26
Identity = 78/243 (32.10%), Postives = 131/243 (53.91%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGI---SVQGGDNG-ASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKE-ATLGDQIVIRPDMKSV-KKNIKKAGFTKSESGKIS----TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII 1062
            L R  L  + ++IAINKP+ +   ++ G  N  A    +L  +   +    +LH+ H+L+K+TTGVL+LA+  ++A  ++   +   +   YL +T G+P    G+I+  + E   +G Q   +  +  + K N    G T+  + + +    T + VL +    SLV+   ++GVK+Q+R H+A  L  PILGDHKYS   K     + PQ+L    L  L L Q K R+LP+HLH+ ++++
Sbjct:   87 LYRSSLYEDQNIIAINKPYDVPLHNINGIRNSIAECLPLLAKITDNMRPGSQLHLCHKLEKETTGVLILAKTEEAAEHVQTLIQSHKVEMKYLAITVGVPVPSEGVIDIPVIEREVVGPQPHFKMALSPLFKVNEDGDGVTRVRAHRQAHAAVTRYQVLDNTSGCSLVELQPLTGVKNQLRVHMALALTCPILGDHKYSHWSK-----LAPQKLPEGTLRRLGLVQSKTRYLPLHLHSRRIVL 324
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Zebrafish
Match: rpusd2 (RNA pseudouridine synthase domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-120411-43])

HSP 1 Score: 59.6918 bits (143), Expect = 3.446e-9
Identity = 45/140 (32.14%), Postives = 64/140 (45.71%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            H VHRLD+ T+GVLL A+  + +  L    +ER + K Y+C   G          F   E    + I++     S +  + +      ES    T F  L  N   S+V+C  ++G  HQIR HL + L  PIL D  Y 
Sbjct:  296 HTVHRLDRLTSGVLLFARTLEVSKKLDVMVRERQLEKEYVCRVEG---------EFPEAEVVCEEPILV----VSFRVGLCRVDPKGKES---RTIFQRLSFNGRSSVVRCRPLTGRTHQIRVHLQF-LGFPILNDPIYG 418
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Xenopus
Match: ENSXETT00000035080.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:8197505:8208230:1 gene:ENSXETG00000011450.1 transcript:ENSXETT00000035080.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 86.6557 bits (213), Expect = 2.980e-18
Identity = 60/198 (30.30%), Postives = 92/198 (46.46%), Query Frame = 1 
Query:  514 IVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNY-------NISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSS 1086
            + HRLD+DTTG L+LA+  ++A  ++ A +ER + + Y     G      G++   +     G     R   ++    +      + E+ ++    +     Y         +++     +GVKHQ+R HLA GL  PILGDHKYS   +     + PQ+     L  L LT  + R L +HLHA QL      LPSS
Sbjct:   99 LCHRLDRDTTGALILARTTEAAERVQQALREREVHRVYWWKCTGCTGSAQGVLGIRVSRKRTG--CTDRGYGRAGSAQVHVGDTGEQEAHRVYWLVHXQVREYRGRGDSSQYNMMDIIPPTGVKHQLRVHLALGLNCPILGDHKYSHWGR-----LAPQKPPDSVLRALGLTVPQARTLSLHLHAVQLT-----LPSS 284
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Xenopus
Match: RPUSD3 (RNA pseudouridine synthase D3 [Source:NCBI gene;Acc:100380049])

HSP 1 Score: 78.5666 bits (192), Expect = 2.674e-15
Identity = 88/316 (27.85%), Postives = 139/316 (43.99%), Query Frame = 1 
Query:  322 FDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAF----KERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSW----LIKENKFPIM------PQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII-GEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLNDSK 1224
            F   K+S++ I  +L    +     ++AINKP G+S+ G +   S+  +L  L   L L   LHIV    K+++G++LL+    +   L+D +    K +    +Y  +  G+PH   G I   LK   + D  ++ P     K  ++     + E  +  T + VL S    +LVQ   +S  + Q+  H    +   ILGDH YS     ++ EN F  M       Q L    L  +  TQ ++  +P+HLH  QL+I GE     S  +G   L+                 A  P FF R+L+ L L  +K
Sbjct:  108 FVVDKMSRERICQLLVENVVYLKGPLVAINKPQGLSITGKEQEVSVSSLLADLQQHLGLGSDLHIVKAAPKESSGLVLLSTCHMTTKRLEDYYALCRKAQKPTASYCAVMAGVPHPAEGEIKAALKLERIEDWQLVVPVTDPSKGRLE-----RREIKRTLTRYKVLDSADGCALVQLQPMSVFQAQLLVHSTL-MFCRILGDHTYSARVGKVLGENIFVPMDIALPRTQVLEENVLRRMHFTQQQMHRMPLHLHFQQLMIPGE-----SPAEGDTFLD-----------------APPPPFFQRTLQLLGLYANK 395
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Xenopus
Match: RPUSD2 (RNA pseudouridylate synthase domain containing 2 [Source:NCBI gene;Acc:100497757])

HSP 1 Score: 58.151 bits (139), Expect = 1.295e-8
Identity = 42/139 (30.22%), Postives = 63/139 (45.32%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            H +HRLD+ T+GVL+ A+  + +  + +  ++R + K Y+C   G          F  +E T  + I +      V +   K    K       T F  L  N   S+V+C   +G  HQIR HL Y L  PI+ D  Y
Sbjct:  215 HTIHRLDRMTSGVLMFAKTLEVSKKIDEQVRQRQLEKEYVCRVCG---------EFPKEEVTCEEPIFVVSYKIGVCRVDPKGKPCK-------TVFQRLSYNGKSSVVKCFPYTGRTHQIRVHLQY-LGHPIVNDPIY 336
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Xenopus
Match: RPUSD3 (RNA pseudouridine synthase D3 [Source:NCBI gene;Acc:100380049])

HSP 1 Score: 56.9954 bits (136), Expect = 2.506e-8
Identity = 50/184 (27.17%), Postives = 86/184 (46.74%), Query Frame = 1 
Query:  322 FDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAF----KERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGV 861
            F   K+S++ I  +L    +     ++AINKP G+S+ G +   S+  +L  L   L L   LHIV    K+++G++LL+    +   L+D +    K +    +Y  +  G+PH   G I   LK   + D  ++ P     K  ++     + E  +  T + VL S    +LVQ   +SG+
Sbjct:  108 FVVDKMSRERICQLLVENVVYLKGPLVAINKPQGLSITGKEQEVSVSSLLADLQQHLGLGSDLHIVKAAPKESSGLVLLSTCHMTTKRLEDYYALCRKAQKPTASYCAVMAGVPHPAEGEIKAALKLERIEDWQLVVPVTDPSKGRLE-----RREIKRTLTRYKVLDSADGCALVQLQPMSGM 286
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Mouse
Match: Rpusd4 (RNA pseudouridylate synthase domain containing 4 [Source:MGI Symbol;Acc:MGI:1919239])

HSP 1 Score: 128.642 bits (322), Expect = 6.994e-33
Identity = 98/293 (33.45%), Postives = 148/293 (50.51%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQGGDN---GASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL-GDQ----IVIRPDMKSVKKNIKKAGFTKSESGKIS-TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
            L+R  L  +N ++ INKP+G+ V GG       S    +          E LH+ HRLDK+TTGV++LA   D A  +++ F+ R + K Y  +T  +P    G+++  +KE  + G Q    + + P  +    N K      S    ++ T + VL S  + +LV+   ++G+KHQ+R HL++GL+ PILGDHKYS   +     + PQ+LS   L  L L Q K R++P+HLHA QLI+  P L S                   R  +  +   +P FF RSL  L L+
Sbjct:   95 LSRRILHQDNDLVVINKPYGLPVHGGPGVQLCISDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWEKDMAHQVQELFRTRQVEKKYWAITVRVPLPSAGVVDIPIKEKEVQGPQQHHKMTLSPSYRL--DNGKMVKVRASRDAHVAVTQYQVLSSASSSALVELQPVTGIKHQLRVHLSFGLDCPILGDHKYSDWTR-----LAPQKLSAGTLKKLGLQQSKARYIPLHLHARQLIL--PALGS-------------------RTEELLLTCKLPHFFARSLLRLGLD 359
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Mouse
Match: Rpusd3 (RNA pseudouridylate synthase domain containing 3 [Source:MGI Symbol;Acc:MGI:2141440])

HSP 1 Score: 72.4034 bits (176), Expect = 1.441e-13
Identity = 79/315 (25.08%), Postives = 145/315 (46.03%), Query Frame = 1 
Query:  301 DYKFVNDFDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXX-XXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAF----KERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQ-LSPVALTGLK---LTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLND 1218
            D  F      + L  +E++ +L    +     ++ +NKP G+ V G     ++  VL  L+  L L+ + L +V    K+ +G++LL+  P +A  L+  F    + +     Y  +T GIP    G +   LK   + D  +  P M   +K+I++ G  ++ S      F+V+ +    +LVQ   ++   +Q++ H+A  L  PILGDH Y+  +      ++ Q+ L P   T  +   L +  +RHL   L   Q+     ++P  L+  +  L    + + P  L      A +P +F R+L+ L+L+ 
Sbjct:   51 DQPFPGLLRTENLGLEELAHVLRAAVVDQKGPLVTLNKPQGLPVTGRPGELTLLSVLPQLSQALGLEHQELQVVRAPGKEASGLVLLSSCPQTASRLQKFFIHSRRAQRPTATYCAVTDGIPEPSEGTVCMPLKMEQMNDVDLAVPVMSPSRKDIQE-GVKRTLS-----RFHVMATGRGCALVQLQPLTVFPNQLQVHMALQL-CPILGDHTYAARVGT----VLGQRFLWPAETTKPQRQVLDEALLRHL--RLSPSQVA----QMPLHLHLHRLLLPGTGSRDPPSEL-----LAPLPPYFSRTLQCLRLSQ 343
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Mouse
Match: Rpusd3 (RNA pseudouridylate synthase domain containing 3 [Source:MGI Symbol;Acc:MGI:2141440])

HSP 1 Score: 70.0922 bits (170), Expect = 4.410e-13
Identity = 57/215 (26.51%), Postives = 103/215 (47.91%), Query Frame = 1 
Query:  301 DYKFVNDFDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXX-XXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAF----KERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            D  F      + L  +E++ +L    +     ++ +NKP G+ V G     ++  VL  L+  L L+ + L +V    K+ +G++LL+  P +A  L+  F    + +     Y  +T GIP    G +   LK   + D  +  P M   +K+I++ G  ++ S      F+V+ +    +LVQ   ++   +Q++ H+A  L  PILGDH Y+
Sbjct:   40 DQPFPGLLRTENLGLEELAHVLRAAVVDQKGPLVTLNKPQGLPVTGRPGELTLLSVLPQLSQALGLEHQELQVVRAPGKEASGLVLLSSCPQTASRLQKFFIHSRRAQRPTATYCAVTDGIPEPSEGTVCMPLKMEQMNDVDLAVPVMSPSRKDIQE-GVKRTLS-----RFHVMATGRGCALVQLQPLTVFPNQLQVHMALQL-CPILGDHTYA 247
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Mouse
Match: Rpusd3 (RNA pseudouridylate synthase domain containing 3 [Source:MGI Symbol;Acc:MGI:2141440])

HSP 1 Score: 70.0922 bits (170), Expect = 6.757e-13
Identity = 64/250 (25.60%), Postives = 117/250 (46.80%), Query Frame = 1 
Query:  301 DYKFVNDFDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXX-XXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAF----KERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQ-LSPVALTGLKLTQGKIRHLP 1032
            D  F      + L  +E++ +L    +     ++ +NKP G+ V G     ++  VL  L+  L L+ + L +V    K+ +G++LL+  P +A  L+  F    + +     Y  +T GIP    G +   LK   + D  +  P M   +K+I++ G  ++ S      F+V+ +    +LVQ   ++   +Q++ H+A  L  PILGDH Y+  +      ++ Q+ L P   T  +   G + H+P
Sbjct:   51 DQPFPGLLRTENLGLEELAHVLRAAVVDQKGPLVTLNKPQGLPVTGRPGELTLLSVLPQLSQALGLEHQELQVVRAPGKEASGLVLLSSCPQTASRLQKFFIHSRRAQRPTATYCAVTDGIPEPSEGTVCMPLKMEQMNDVDLAVPVMSPSRKDIQE-GVKRTLS-----RFHVMATGRGCALVQLQPLTVFPNQLQVHMALQL-CPILGDHTYAARVGT----VLGQRFLWPAETTKPQRQVGPLSHMP 289
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Mouse
Match: Rpusd2 (RNA pseudouridylate synthase domain containing 2 [Source:MGI Symbol;Acc:MGI:1918066])

HSP 1 Score: 62.3882 bits (150), Expect = 5.371e-10
Identity = 54/184 (29.35%), Postives = 80/184 (43.48%), Query Frame = 1 
Query:  376 FLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
             L  NN V+ I+KP  I V     G      +  +  K    + LH +HRLD+ T+GVL+ A+    +  + +  ++R + K Y+C   G          F  KE    + I++     S K  + +            T F  L  N   S+VQC  ++G  HQIR HL + L  PIL D  Y
Sbjct:  238 LLAENNDVVVIDKPSSIPVH--PCGRFRHNTVIFILGKEHQLKELHPLHRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAG---------EFPDKEVICKEPILV----VSYKVGVCR---VDPRGKPCETVFQRLSYNGRSSVVQCRPLTGRTHQIRVHLQF-LGHPILNDPIY 402
[Close]
BLAST of RNA pseudouridine synthase D4 vs. UniProt/SwissProt
Match: sp|Q4QQT0|RUSD4_RAT (Mitochondrial RNA pseudouridine synthase Rpusd4 OS=Rattus norvegicus OX=10116 GN=Rpusd4 PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.329e-38
Identity = 100/294 (34.01%), Postives = 149/294 (50.68%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQGGDN---GASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQ-----IVIRPDMKSVKKNIKKAGFTKSESGKIS-TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
            L+R  L  +  ++ INKP+G+ V GG       S    +          E LH+ HRLDK+TTGV++LA   D A  +++ F+ R + K Y  +T  +P    G+++  + E  +  Q     + +RP  +    N K      S    ++ T + VL S  + +LV+   ++G+KHQ+R HLA+GL+ PILGDHKYS W    N+  + PQ+LS   L  L L Q K R++P+HLHA QLI+  P L S                   R  +  +   +P FF RSLR L L+
Sbjct:   95 LSRRILHQDKDLVVINKPYGLPVHGGPGVQLCISDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWEKDMAHQVQELFRTRQVEKKYWAITVRVPLPSAGVVDIPIMEKEVAGQQQHHKMTLRPSYRM--DNGKMVKVRASRDAHVAVTQYQVLSSTPSSALVELQPVTGIKHQLRVHLAFGLDCPILGDHKYSDW----NR--LAPQKLSAGTLKKLGLQQSKARYIPLHLHARQLIL--PALGS-------------------RTEELLLACKLPHFFARSLRRLGLD 359
[Close]
BLAST of RNA pseudouridine synthase D4 vs. UniProt/SwissProt
Match: sp|Q96CM3|RUSD4_HUMAN (Mitochondrial RNA pseudouridine synthase RPUSD4 OS=Homo sapiens OX=9606 GN=RPUSD4 PE=1 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 1.271e-36
Identity = 98/308 (31.82%), Postives = 158/308 (51.30%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXX----XXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQ-----IVIRPDMKSVKKNIKKAGFTKSESGKIS-TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
              +++  + ++  L R  L  + +++ INKP+G+ V GG  G  +     L            E LH+ HRLDK+TTGV++LA + D A  +++ F+ R + K Y  +T  +P    G+++  + E     Q     + + P  +    + K     +S + +++ T + VL S  + +LV+   I+G+KHQ+R HL++GL+ PILGDHKYS W    N+  + PQ+LS   L  L L Q K R++P+HLHA QLI+       +L  GK  LN               +   +P FF+ SL  L+L 
Sbjct:   82 QLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGP-GVQLCITDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQGQQQHHKMTLSPSYRM--DDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLDCPILGDHKYSDW----NR--LAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLIL------PALGSGKEELN---------------LVCKLPRFFVHSLHRLRLE 359
[Close]
BLAST of RNA pseudouridine synthase D4 vs. UniProt/SwissProt
Match: sp|Q9CWX4|RUSD4_MOUSE (Mitochondrial RNA pseudouridine synthase Rpusd4 OS=Mus musculus OX=10090 GN=Rpusd4 PE=2 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 4.892e-32
Identity = 98/293 (33.45%), Postives = 148/293 (50.51%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQGGDN---GASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL-GDQ----IVIRPDMKSVKKNIKKAGFTKSESGKIS-TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
            L+R  L  +N ++ INKP+G+ V GG       S    +          E LH+ HRLDK+TTGV++LA   D A  +++ F+ R + K Y  +T  +P    G+++  +KE  + G Q    + + P  +    N K      S    ++ T + VL S  + +LV+   ++G+KHQ+R HL++GL+ PILGDHKYS   +     + PQ+LS   L  L L Q K R++P+HLHA QLI+  P L S                   R  +  +   +P FF RSL  L L+
Sbjct:   95 LSRRILHQDNDLVVINKPYGLPVHGGPGVQLCISDVLPILAKMLHGHKAEPLHLCHRLDKETTGVMVLAWEKDMAHQVQELFRTRQVEKKYWAITVRVPLPSAGVVDIPIKEKEVQGPQQHHKMTLSPSYRL--DNGKMVKVRASRDAHVAVTQYQVLSSASSSALVELQPVTGIKHQLRVHLSFGLDCPILGDHKYSDWTR-----LAPQKLSAGTLKKLGLQQSKARYIPLHLHARQLIL--PALGS-------------------RTEELLLTCKLPHFFARSLLRLGLD 359
[Close]
BLAST of RNA pseudouridine synthase D4 vs. UniProt/SwissProt
Match: sp|Q5E9Z1|RUSD4_BOVIN (Mitochondrial RNA pseudouridine synthase RPUSD4 OS=Bos taurus OX=9913 GN=RPUSD4 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 1.526e-30
Identity = 92/307 (29.97%), Postives = 153/307 (49.84%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDN---GASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQ-----IVIRPDMKSVKKNIKKAGFTKSESGKIS-TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
              +++  + ++  L+R  +  +  ++ INKP+G+ V GG       S    +          + LH+ HRLDK+TTGV++LA   + A  +++ F+ R + K Y  +T  +P  E G+++  + E     Q     + + P  +    + K      S + +++ T + VL S+ + +L++   I+G+KHQ+R H+++GL+ PILGDHKYS W    N+  + PQ+LS   L  L L Q K RHLP+HLHA QL +       +L   K  L                +    P +F+ SLR L L 
Sbjct:   82 QLQRVHPNVLAKALSRGIVHQDKELVVINKPYGLPVHGGPGVKLCISDVLPVLAKILHGPKAKPLHLCHRLDKETTGVMVLAWEKEVAHQVQELFRTRQVTKKYWAITVRVPVPEAGVVDIPIVEKEAQGQQHHHKMTLSPSYRM--DDGKMVRVRSSRNAQLAVTQYQVLSSSLSAALLELQPITGIKHQLRVHMSFGLDCPILGDHKYSDW----NR--LAPQKLSAGILKKLGLQQSKARHLPLHLHACQLTL------PALQPQKEELT---------------LLCRPPRYFVNSLRRLGLK 359
[Close]
BLAST of RNA pseudouridine synthase D4 vs. UniProt/SwissProt
Match: sp|Q28C59|RUSD4_XENTR (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 4.844e-29
Identity = 86/280 (30.71%), Postives = 135/280 (48.21%), Query Frame = 1 
Query:  295 KADYKFVNDFDFKKLSKDEISVILARCF----LKSNNHVIAINKPHGISVQGG---DNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKE-ATLGDQ----IVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDG 1098
            +A+ K +     +   K+  +++  R      ++ +  ++ +NKPHG+ V GG   +   +               E L + HRLD+DTTG L+LA+  ++A  ++ A +ER + + Y  L  G P    GI++  + E  T G Q    + + P  +  ++   +       + +  T +  L +    SLV+   I+GVKHQ+R HLA GL  PILGDHKYS   +     + PQ+     L  L LT  + R L +HLHA QL      LPSS  DG
Sbjct:   20 RAERKVLEGAARETSRKNAPTIVYLRELKSQVVREDPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAEPLKLCHRLDRDTTGALILARTTEAAERVQQALREREVHRVYWALCLGTPSPREGILDIPIMEKETSGPQKHYKMALSPRFRVSEEGAVERVRVPRSAHEAVTRYRTLGAASGASLVELHPITGVKHQLRVHLALGLNCPILGDHKYSHWGR-----LAPQKPPDSVLRALGLTVPQARTLSLHLHAVQLT-----LPSS--DG 287
[Close]
BLAST of RNA pseudouridine synthase D4 vs. TrEMBL
Match: A0A210QD54 (RNA pseudouridylate synthase domain-containing protein 4 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT16120 PE=4 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 2.123e-43
Identity = 110/298 (36.91%), Postives = 165/298 (55.37%), Query Frame = 1 
Query:  334 KLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXX---HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLG--DQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKL 1212
            K++  EI  ++ R  L  ++ V+AI KP+G+   GG  G +I     +     SL ER    H+VHRLDKDTTGV+LLA+    A  L  AF ER + K Y  +T GIPH + GII+  + E ++    ++V++P+     +   K+     ++ K  T + V+ S  N +LV+C  ++GV+HQ+RAHL++GL TP+LGDHKYS L+        PQ+L    L  L + Q K+RH+P+HLHA  ++I E        DG+                +  V +N+P  F RS+R+LKL
Sbjct:  612 KMTSVEIVNMITRHILYDSDDVVAIYKPYGLPSHGGP-GVAISVAHLMDQISESLGERQNPLHMVHRLDKDTTGVMLLARTKHMAAQLAKAFAERTVVKKYWAITKGIPHPKEGIIDIPMMEGSVDGKKRMVLKPNFLPDGRIATKSSV---KTFKAVTHYKVISSRDNTALVECLPLTGVRHQLRAHLSFGLNTPVLGDHKYSNLLN-----FAPQKLDSTTLRKLGVQQSKVRHIPLHLHAMSILIPE------FLDGR----------------NLFVTSNLPKHFCRSMRNLKL 878
[Close]
BLAST of RNA pseudouridine synthase D4 vs. TrEMBL
Match: A0A1D1VNM8 (PseudoU_synth_2 domain-containing protein OS=Ramazzottius varieornatus OX=947166 GN=RvY_13003-1 PE=4 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.468e-43
Identity = 101/306 (33.01%), Postives = 164/306 (53.59%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNG----ASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGD--QIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLNDSK 1224
            D+ K+  D++  +L R  + + + ++AINKP+G+ V GG +     A     L  +       E LH+VHR+DK+TTGVLLLA+  + A  + D F +R++RK Y  LT G+P    GIIN  + E+      + V+RP    V   ++K   T+ +  +  T + VL +    +LV+    +GVKHQ+R HLA+G+ TP+LGDHKYS   K     + PQ+L P  L    + Q K+R +P+HLHA  +++ E        DG+                +  + A +P++F +S++ L++  +K
Sbjct:  209 DWNKMIADDVVNMLKRSIIYNEHDIVAINKPYGLPVHGGPSVHHHVARYLPKLAEILDPTKKLETLHMVHRIDKETTGVLLLARTAERAAQIHDLFLKRNVRKIYWVLTKGVPDPLKGIINIPIGESDFSGTHRKVLRPGPSPVTSLVRKGRRTRDDGREAITNYTVLGATKECALVELNTETGVKHQVRVHLAHGISTPVLGDHKYSHFTK-----LAPQKLPPSFLKAFDIRQQKVRTIPMHLHAMSVVLPE------FIDGQ----------------NVHISAPLPEYFTQSMKKLRVRKNK 487
[Close]
BLAST of RNA pseudouridine synthase D4 vs. TrEMBL
Match: C3XV24 (PseudoU_synth_2 domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_126415 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 8.013e-41
Identity = 109/316 (34.49%), Postives = 160/316 (50.63%), Query Frame = 1 
Query:  289 KQKADYKFVNDF-DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQ------IVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
            ++K  YK+ +   D   +S   ++ IL +  L     ++AI KP+G+ V GG            L  +    +RL++VHRLDK+TTGV+LLA+  D A  L   F+ R + K Y  LT G+P  E G+++  +KE  +  Q      +V  P+     K I K     S++    T F VL S  + +LV+C   +GVKHQIR+HL+YGL  PILGDHKYS   K     + PQ+L    L    + Q K R+LP+HLHA Q+++   R   SLY                        A +PDFF R+++ L++ 
Sbjct:   41 REKFLYKWKHQVADLTHVSPYTLASILKKSLLYDEGDIVAIEKPYGVPVHGGPGMGVSIADSLPLLQQALKTDRLYLVHRLDKETTGVMLLAKTDDMARQLHSLFRLRKVIKKYWVLTMGVPSHEEGVVDIPIKELEVTAQGRPHHRMVCVPEYSEETKLIVKKKRKSSDAQIAITNFRVLDSCGSCALVECQPETGVKHQIRSHLSYGLGCPILGDHKYSHHSK-----LAPQRLPTGMLERFGIQQPKARYLPMHLHARQIVLPGFRDGRSLY----------------------FTARLPDFFNRNVKKLRIR 329
[Close]
BLAST of RNA pseudouridine synthase D4 vs. TrEMBL
Match: A0A0L8GZN6 (PseudoU_synth_2 domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22025471mg PE=4 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 2.423e-40
Identity = 111/304 (36.51%), Postives = 178/304 (58.55%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDN-GASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLG--DQIVIRPD-MKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLNDSK 1224
            D  KL+  EI   +    + + N ++AINKP+G+S  GG     S+ ++L  LAAK+  K  LH++HRLDK+TTGVLLLA+  + A +L   F+E+ + K Y  +T G+P+ + GII+  + E ++    ++V++PD  +  +  +KK+   + E+    T + VLC +Y  +LV+C   +GVKHQ+R HLA+GL TPI+GDHKYS + K     + PQ+L P  L  L + Q K+R++P+HLH+  +I+ +      L DGK                +  + A++P  F ++++SLKL   K
Sbjct:  353 DLTKLTSYEIVNHIKSRIIYNKNDIVAINKPYGLSSHGGPGVHVSLADLLPELAAKIYHKGTLHLIHRLDKETTGVLLLARTAEMAAVLHKMFREQEVIKKYWAITKGVPNPDIGIIDIPIAEGSIDGKSRMVLKPDYTEETRLVMKKSSVKRFEA---VTRYKVLCKSYESALVECQPKTGVKHQVRVHLAFGLNTPIIGDHKYSHINK-----LAPQRLFPEILQRLNVRQSKVRYIPMHLHSKIIILPQ------LLDGK----------------NLFIKADLPYHFRKNMKSLKLKADK 626
[Close]
BLAST of RNA pseudouridine synthase D4 vs. TrEMBL
Match: V4AUI7 (PseudoU_synth_2 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_140139 PE=4 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 8.059e-40
Identity = 106/306 (34.64%), Postives = 162/306 (52.94%), Query Frame = 1 
Query:  325 DFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDN-----GASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQI---VIRPDM-KSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
            D  K  +  +  IL +  + +   +IAINKP+G+   GG       G  + ++ E L  + +  + LH++HRLDK+TTGV+LLA+    A  L++ F +R + K Y  +T GIP    G+I   + E +L + I   +++P+  K   + +KK    +++     T + V   + + +L++C  ISG+KHQIR HL +GL TPILGDHKYS   K     + PQ+L P  LT L +   K+RH+P+HLHA  ++I E       +    NL                V A +P  FL +LR LKLN
Sbjct:   73 DLTKEPEGIVVDILKKNIIYNEGDIIAINKPYGLPSHGGPGVKFSVGGLLHQLAESLNDRDNSLQTLHLIHRLDKETTGVMLLAKRAAVAEELREYFVKRKVVKKYWVITKGIPDPLEGVIKIPITEGSLPNGIHRMILKPEYNKKYWEVMKKRNVEEAQDA--ITQYKVFKHHQSAALIECQPISGIKHQIRCHLGFGLNTPILGDHKYSHFSK-----LAPQRLPPDMLTSLGIRASKVRHVPMHLHAKSIVIPE-------FQNGQNL---------------MVSAKLPKHFLINLRRLKLN 349
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Cavefish
Match: rpusd4 (RNA pseudouridylate synthase domain-containing protein 4-like [Source:NCBI gene;Acc:103024327])

HSP 1 Score: 122.865 bits (307), Expect = 3.443e-31
Identity = 89/245 (36.33%), Postives = 137/245 (55.92%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISV-QGGDNGASIXXXXXXXX---XXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNL--KEATLGDQIVIRPDMKSVKKNIKKA-GFTKSESGKIS----TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII 1062
            L+R  L  + H+IAINKP+G+ V   GD   SI E L +L+   + +    +L + + L+KDTTGVLLLA+  ++   ++   K + +   Y+ +T G+P    G+I+  +  KE T G Q   +  +  + +  ++  G T+    + +    T + VL S+   SLV+   I+GVKHQ+R H+A  L  PILGDHKY+ W    NK  + PQ+L    L  L L Q K R+LP+HLH  Q+I+
Sbjct:   83 LSRSVLYQDQHLIAINKPYGVPVYNSGDIRNSIAESLPVLSKIISGMQAGSQLQLCYSLEKDTTGVLLLARTEEACQRVQSLIKSQQMESRYMVITVGVPVPSEGVIDIPIIEKEVT-GAQHHFKMGLSPLYRVSEEGDGLTRVRPHRQAHGAVTQYRVLDSSSGCSLVELQPITGVKHQLRVHMALALGCPILGDHKYANW----NK--LAPQKLPEGVLRSLSLEQSKTRYLPLHLHVRQIIL 320
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Cavefish
Match: rpusd4 (RNA pseudouridylate synthase domain-containing protein 4-like [Source:NCBI gene;Acc:103024327])

HSP 1 Score: 123.25 bits (308), Expect = 4.800e-31
Identity = 89/245 (36.33%), Postives = 137/245 (55.92%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISV-QGGDNGASIXXXXXXXX---XXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNL--KEATLGDQIVIRPDMKSVKKNIKKA-GFTKSESGKIS----TAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS-WLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLII 1062
            L+R  L  + H+IAINKP+G+ V   GD   SI E L +L+   + +    +L + + L+KDTTGVLLLA+  ++   ++   K + +   Y+ +T G+P    G+I+  +  KE T G Q   +  +  + +  ++  G T+    + +    T + VL S+   SLV+   I+GVKHQ+R H+A  L  PILGDHKY+ W    NK  + PQ+L    L  L L Q K R+LP+HLH  Q+I+
Sbjct:  112 LSRSVLYQDQHLIAINKPYGVPVYNSGDIRNSIAESLPVLSKIISGMQAGSQLQLCYSLEKDTTGVLLLARTEEACQRVQSLIKSQQMESRYMVITVGVPVPSEGVIDIPIIEKEVT-GAQHHFKMGLSPLYRVSEEGDGLTRVRPHRQAHGAVTQYRVLDSSSGCSLVELQPITGVKHQLRVHMALALGCPILGDHKYANW----NK--LAPQKLPEGVLRSLSLEQSKTRYLPLHLHVRQIIL 349
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Cavefish
Match: rpusd2 (RNA pseudouridine synthase domain containing 2 [Source:NCBI gene;Acc:103042945])

HSP 1 Score: 58.5362 bits (140), Expect = 8.043e-9
Identity = 43/140 (30.71%), Postives = 63/140 (45.00%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            H VHRLD+ T+GVLL A+  + +  L    ++R + K Y+C   G          F   E    + I++     S K  + +      +     T F  L  N   S+V+C  ++G  HQIR HL + L  PIL D  Y 
Sbjct:  282 HTVHRLDRLTSGVLLFARTLEVSKKLDQMVRDRQLEKEYVCRVDG---------EFPDGEVVCEEPILV----VSFKVGVCR---VDPKGKDCRTVFQKLSYNGRSSVVRCRPLTGRTHQIRVHLQF-LGFPILNDPIYG 404
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009045.1 (pep scaffold:Pmarinus_7.0:GL484265:15078:19154:1 gene:ENSPMAG00000008183.1 transcript:ENSPMAT00000009045.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 112.464 bits (280), Expect = 4.193e-28
Identity = 87/281 (30.96%), Postives = 137/281 (48.75%), Query Frame = 1 
Query:  361 ILARCFLKSNNH----VIAINKPHGISVQGG-----DNGASIXXXXX---------------XXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEAT------LGDQIVIRPDM-------KSVKKNIKKAGFTKSESGKISTAFNVLC--SNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQ--------GKIRHLPIHLHAYQLII 1062
            +LA+  LK+  H    +I +NKP+GI V GG     +N A++  VLE                 A   +   +LH+ HRLD+DTTG L+LA+N ++A  +    +ER   + Y  +T G+P    G+++  + E        +  ++ + P           VK+ + +   T        T + VL   +    +LV+   ++G KHQ+R HLA GL TP LGDHKYS   +     ++PQ+LSP  L  L L +        G  R +P+HLHA +L++
Sbjct:   32 VLAKALLKNVIHQQRELIVVNKPYGIPVHGGPGVGRNNVAAVLPVLEQLLDGHPSRRRHRKNTTAGDDATASKLHLCHRLDRDTTGALVLARNAEAADAVASLLRERRAERTYWAVTVGVPQPARGVVDLPVGERKHEGPDGVTYRMAVMPRFHYVDASNALVKRRLPRGART------AVTQYRVLAEGAEGRSALVELRPLTGHKHQLRVHLALGLGTPALGDHKYSP--RRQGQGLVPQRLSPALLRKLGLARSGQERPAAGAARRVPLHLHACRLVL 304
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Sea Lamprey
Match: rpusd2 (RNA pseudouridine synthase domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-120411-43])

HSP 1 Score: 53.5286 bits (127), Expect = 3.806e-8
Identity = 46/141 (32.62%), Postives = 66/141 (46.81%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSES-GK-ISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            H VHRLD+ T+GVLL A   ++   L    +ER ++K YLC   G          F   E    +++V    +  V  ++   G  + ++ GK   T F  L  N   S++ C   +G  HQIR HL + L  PIL D  Y
Sbjct:   56 HTVHRLDRLTSGVLLFAHTLEAVQRLDRQIREREVQKEYLCRVEG---------RFPGGEGEEAEEVVCAEPVLVVSYSV---GVCRVDTRGKPCRTTFTRLSYNGRSSVLLCRPETGRMHQIRVHLQF-LGHPILNDPVY 183
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Yeast
Match: PUS6 (tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability [Source:SGD;Acc:S000003401])

HSP 1 Score: 60.4622 bits (145), Expect = 1.700e-10
Identity = 48/145 (33.10%), Postives = 70/145 (48.28%), Query Frame = 1 
Query:  520 HRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHG-IPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNI--------SLVQCAMISGVKHQIRAHLAYGLETPILGDHKY 927
            +RLDK T+G+L+LA+N  SA  ++ + + R + K YL    G  PH E  + N N  E T  D   +  +M  +     K  F       +ST+ + +   Y I        ++V C  I+G  HQIR HLA  L  PI+ D  Y
Sbjct:  165 YRLDKITSGLLILAKNSQSAGEIQKSIRSRDMIKIYLARVKGRFPHSELILDNENAAETTFEDTSKVTVEMTPIYSIDPKRQFPVG----LSTSKDAITKFYPIRYFSHADETVVACKPITGRTHQIRIHLA-RLGHPIVNDSVY 304
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Yeast
Match: RIB2 (Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication [Source:SGD;Acc:S000005427])

HSP 1 Score: 58.9214 bits (141), Expect = 7.498e-10
Identity = 41/140 (29.29%), Postives = 64/140 (45.71%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            H  +RLDK T+G++ LA+ P  A  + D  K R + K Y+    G      GI+  +    ++  ++ +    +   +N K A           T F  +  +   S+V+C  ++G  HQIR HL Y L  PI  D  YS
Sbjct:  205 HPCNRLDKPTSGLMFLAKTPLGADRMGDQMKAREVTKEYVARVKG--EFPIGIVEVDKPVRSVNPKVALNAVCEMSDENAKHA----------KTVFQRVSYDGQTSIVKCKPLTGRTHQIRVHLQY-LGFPIANDPIYS 331
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Yeast
Match: PUS9 (Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication [Source:SGD;Acc:S000002194])

HSP 1 Score: 58.5362 bits (140), Expect = 7.684e-10
Identity = 45/139 (32.37%), Postives = 62/139 (44.60%), Query Frame = 1 
Query:  520 HRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLK--EATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            +RLD+ T+G++ LA+ P  A  + D  K R + K Y+    G   E   I+   LK  E  L    V + D K  K                 T FN +  +   S+V+C  ++G  HQIR HL Y L  PI  D  YS
Sbjct:  235 NRLDRLTSGLMFLAKTPKGADNIGDQLKAREVTKEYVAKVVGEFPETEVIVEKPLKLIEPRLALNAVCQMDEKGAK--------------HAKTVFNRISYDGKTSIVKCKPLTGRSHQIRVHLQY-LGHPIANDPIYS 358
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Nematostella
Match: EDO47554 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RM79])

HSP 1 Score: 118.242 bits (295), Expect = 3.866e-30
Identity = 91/255 (35.69%), Postives = 143/255 (56.08%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQGGDNGA-SIXXXXXXXXXXXXXXXXXHIVHRLDK----------DTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLG--DQIVIRPDM-KSVKKNIKKAGFTKSESGKISTAFNVLCSNYNI-SLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENK-FPIMPQQLSPVA-LTGLKLTQGKIR-----HLPIHLHAYQLII 1062
            LA   + SNN +IAINKP+G+ V GG     S+ E+LE  AA  + + +  + HRLDK          D +GVLLL +N  +A  + + F+ R ++K YL ++ G+P  E G I+  +    +G  ++I+IR D+     K I +AGF ++      T F +L  N +  SL++   I+G K QIR HLA  L  PILGDHK+S    + +  P+   QL  ++ + G+   +G+IR      +P+HLH++QL++
Sbjct:   21 LADNVIFSNNDLIAINKPYGLPVHGGPGVVLSVAELLEEFAAIKNYQNKPELAHRLDKYGTDYTIMLIDCSGVLLLTRNKTAAQKIAEMFQSRMVKKRYLAVSVGVPRYEEGTISIPVGYGRVGPWERIIIRQDLVGKTHKEITEAGFKQA-----ITKFKILDENQSTCSLLEIEPITGHKQQIRVHLADALRCPILGDHKFSSDKPQPQVIPLRLLQLLQISGVKGVDGKKGRIRPWQRGQIPLHLHSHQLVL 270
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Nematostella
Match: EDO47303 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMP5])

HSP 1 Score: 61.2326 bits (147), Expect = 3.002e-10
Identity = 48/162 (29.63%), Postives = 72/162 (44.44%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHG-IPHE----EHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY---SWLIKENKFPIMPQQ 972
            + +HR+D+ T+G+L+ A+    A +L+   ++R + K YLC   G  P E    E  II  + K         + PD KS +                 T F  L SN   S+V+C   +G  HQIR HL + L  PI+ D  Y   +W  K  +  +   Q
Sbjct:  133 YTIHRIDRLTSGILMFAKTLTRARMLEAQVRDRQVEKEYLCRVQGEFPSEPVDCEQPIIVVSHKVGIC----RVSPDGKSCR-----------------TVFTKLHSNGKSSVVKCIPYTGRMHQIRVHLQW-LGYPIIDDPIYNHPAWGTKRGRGGVTDDQ 272
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Medaka
Match: rpusd4 (mitochondrial RNA pseudouridine synthase rpusd4-like [Source:NCBI gene;Acc:101164300])

HSP 1 Score: 117.087 bits (292), Expect = 4.679e-29
Identity = 93/294 (31.63%), Postives = 150/294 (51.02%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQGGDNG-ASIXXXXXXXXXXXXXXXXXHIVH--RLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL-GDQIVIRPDMKSVKKNIKKAG------FTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLN 1215
            L R  L ++  V+ I+KPHG+ V+G  N  +S+  VL  + + + ++    ++    L+KD+TG LLLA+N D    +    +   +++ Y  +  G+P    G+++  + E  + G Q   + D+  + + +  AG        + ++    T + VL S    SLV+    + VKHQ+R H+A  L+ PILGDHKYS     NK  + PQ+L    L  L L Q +IR+LP+HLHA QLI     LP+    G+A               D +V   +P +FL++L  L+LN
Sbjct:   72 LRRSVLYADEDVVVISKPHGVPVRGQSNSISSVLPVLSKMMSGMKVRSESQLLPCLGLEKDSTGALLLARNEDVVEHILSLRRNNQVQRKYWVVAVGVPVPSEGLVDIPIIEREVAGSQPHFKMDLSPLFR-MSDAGDGLIRVRGQRQAHPAVTKYRVLESGGGCSLVELQPFTEVKHQMRVHMAIALDCPILGDHKYSH---RNK--LAPQKLPEHMLKKLGLEQSQIRYLPLHLHARQLI-----LPAR--SGQA---------------DIRVSCPLPKYFLQTLNRLRLN 337
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Medaka
Match: rpusd4 (mitochondrial RNA pseudouridine synthase rpusd4-like [Source:NCBI gene;Acc:101164300])

HSP 1 Score: 113.235 bits (282), Expect = 2.612e-27
Identity = 91/297 (30.64%), Postives = 152/297 (51.18%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQ---GGDNGASIXXXXXXXXXXXXXXXXXHIVH--RLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL-GDQIVIRPDMKSVKKNIKKAG------FTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLND 1218
            L R  L ++  V+ I+KPHG+ V+   G ++ +S+  VL  + + + ++    ++    L+KD+TG LLLA+N D    +    +   +++ Y  +  G+P    G+++  + E  + G Q   + D+  + + +  AG        + ++    T + VL S    SLV+    + VKHQ+R H+A  L+ PILGDHKYS     NK  + PQ+L    L  L L Q +IR+LP+HLHA QLI     LP+    G+A               D +V   +P +FL++L  LK+++
Sbjct:  118 LRRSVLYADEDVVVISKPHGVPVREDAGANSISSVLPVLSKMMSGMKVRSESQLLPCLGLEKDSTGALLLARNEDVVEHILSLRRNNQVQRKYWVVAVGVPVPSEGLVDIPIIEREVAGSQPHFKMDLSPLFR-MSDAGDGLIRVRGQRQAHPAVTKYRVLESGGGCSLVELQPFTEVKHQMRVHMAIALDCPILGDHKYSH---RNK--LAPQKLPEHMLKKLGLEQSQIRYLPLHLHARQLI-----LPA--RSGQA---------------DIRVSCPLPKYFLQTLNRLKISE 386
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Medaka
Match: rpusd4 (mitochondrial RNA pseudouridine synthase rpusd4-like [Source:NCBI gene;Acc:101164300])

HSP 1 Score: 109.768 bits (273), Expect = 2.697e-26
Identity = 90/293 (30.72%), Postives = 148/293 (50.51%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPHGISVQ---GGDNGASIXXXXXXXXXXXXXXXXXHIVH--RLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL-GDQIVIRPDMKSVKKNIKKAG------FTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSL 1206
            L R  L ++  V+ I+KPHG+ V+   G ++ +S+  VL  + + + ++    ++    L+KD+TG LLLA+N D    +    +   +++ Y  +  G+P    G+++  + E  + G Q   + D+  + + +  AG        + ++    T + VL S    SLV+    + VKHQ+R H+A  L+ PILGDHKYS     NK  + PQ+L    L  L L Q +IR+LP+HLHA QLI     LP+    G+A               D +V   +P +FL++L  L
Sbjct:   93 LRRSVLYADEDVVVISKPHGVPVREDAGANSISSVLPVLSKMMSGMKVRSESQLLPCLGLEKDSTGALLLARNEDVVEHILSLRRNNQVQRKYWVVAVGVPVPSEGLVDIPIIEREVAGSQPHFKMDLSPLFR-MSDAGDGLIRVRGQRQAHPAVTKYRVLESGGGCSLVELQPFTEVKHQMRVHMAIALDCPILGDHKYSH---RNK--LAPQKLPEHMLKKLGLEQSQIRYLPLHLHARQLI-----LPAR--SGQA---------------DIRVSCPLPKYFLQTLNRL 357
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Medaka
Match: rpusd4 (mitochondrial RNA pseudouridine synthase rpusd4-like [Source:NCBI gene;Acc:101164300])

HSP 1 Score: 100.138 bits (248), Expect = 6.896e-23
Identity = 87/291 (29.90%), Postives = 144/291 (49.48%), Query Frame = 1 
Query:  364 LARCFLKSNNHVIAINKPH-GISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVH--RLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL-GDQIVIRPDMKSVKKNIKKAG------FTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSL 1206
            L R  L ++  V+ IN P   ++  G ++ +S+  VL  + + + ++    ++    L+KD+TG LLLA+N D    +    +   +++ Y  +  G+P    G+++  + E  + G Q   + D+  + + +  AG        + ++    T + VL S    SLV+    + VKHQ+R H+A  L+ PILGDHKYS     NK  + PQ+L    L  L L Q +IR+LP+HLHA QLI     LP+    G+A               D +V   +P +FL++L  L
Sbjct:  110 LRRSVLYADEDVVVINDPTPNVNDAGANSISSVLPVLSKMMSGMKVRSESQLLPCLGLEKDSTGALLLARNEDVVEHILSLRRNNQVQRKYWVVAVGVPVPSEGLVDIPIIEREVAGSQPHFKMDLSPLFR-MSDAGDGLIRVRGQRQAHPAVTKYRVLESGGGCSLVELQPFTEVKHQMRVHMAIALDCPILGDHKYS---HRNK--LAPQKLPEHMLKKLGLEQSQIRYLPLHLHARQLI-----LPAR--SGQA---------------DIRVSCPLPKYFLQTLNRL 372
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Medaka
Match: rpusd2 (RNA pseudouridine synthase domain containing 2 [Source:NCBI gene;Acc:101173104])

HSP 1 Score: 63.1586 bits (152), Expect = 2.186e-10
Identity = 44/140 (31.43%), Postives = 66/140 (47.14%), Query Frame = 1 
Query:  511 HIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYS 930
            H VHRLD+ T+GVLL A+  +++  L    ++R I K Y+C   G          F   E    + I++     S K  + +      +  +  T F  L  N   S+V+C+ ++G  HQIR HL + L  PIL D  Y 
Sbjct:  267 HTVHRLDRLTSGVLLFARTVETSKKLDKMVRDREIEKEYVCRVEG---------EFPEGELVCEEPILV----VSFKVGLCRV---DPKGKECRTVFQRLSFNGKTSVVRCSPLTGRTHQIRVHLQF-LGFPILNDPIYG 389
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Planmine SMEST
Match: SMESG000060928.1 (SMESG000060928.1)

HSP 1 Score: 746.503 bits (1926), Expect = 0.000e+0
Identity = 383/385 (99.48%), Postives = 383/385 (99.48%), Query Frame = 1 
Query:  100 MDKLNILGLKPNESYQLDTNLENNDKKSNHTEDRSSAAEFASFIRDQSKTNIHLNLFSKSHNLKQKADYKFVNDFDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIXXXXXXXXXXXXXXXXXHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFLRSLRSLKLNDSKIFKFALKQIL 1254
            MDKLNILGLKPNESYQLDTNLENNDKKSNHTEDRSSAAEFASFIRDQSKTNIHLNLFSKSHNLKQKADYKFVNDFDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIEEVLELLAAKLSLKERLHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSE GKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFF RSLRSLKLNDSKIFKFALKQIL
Sbjct:    1 MDKLNILGLKPNESYQLDTNLENNDKKSNHTEDRSSAAEFASFIRDQSKTNIHLNLFSKSHNLKQKADYKFVNDFDFKKLSKDEISVILARCFLKSNNHVIAINKPHGISVQGGDNGASIEEVLELLAAKLSLKERLHIVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSEPGKISTAFNVLCSNYNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGLKLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLPDFQVYANVPDFFFRSLRSLKLNDSKIFKFALKQIL 385
[Close]
BLAST of RNA pseudouridine synthase D4 vs. Planmine SMEST
Match: SMESG000051935.1 (SMESG000051935.1)

HSP 1 Score: 57.3806 bits (137), Expect = 1.102e-8
Identity = 50/147 (34.01%), Postives = 74/147 (50.34%), Query Frame = 1 
Query:  514 IVHRLDKDTTGVLLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEE-HGIINFNLKEATLGDQIVIRPDMKSVKKNIKKAGFTKSESGKI-STAFNVLCSN--YNISLVQCAMISGVKHQIRAHLAYGLETPILGDHKY---SW 933
            ++HR+D+ T+G+LL  ++ +++  L    ++R + K YL +  G  +EE    I  N     L  ++ +   +KSV+           E GKI ST F  L  N   N+SLV C  ++G  HQIR HL Y L  PI  D  Y   SW
Sbjct:  183 VIHRIDRMTSGILLFGKDYETSSKLSGQIRDRQVLKYYLAVVGGKFNEEKEQPIECNKPLGILCPKVGL-AYLKSVE-----------EGGKIASTKFMFLKYNEKQNLSLVLCKPLTGRTHQIRVHLQY-LGFPISNDPLYNCISW 316
The following BLAST results are available for this feature:
BLAST of RNA pseudouridine synthase D4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPUSD42.646e-3731.82RNA pseudouridine synthase D4 [Source:HGNC Symbol;... [more]
RPUSD42.234e-3531.46RNA pseudouridine synthase D4 [Source:HGNC Symbol;... [more]
RPUSD21.501e-927.72RNA pseudouridine synthase domain containing 2 [So... [more]
RPUSD21.681e-927.72RNA pseudouridine synthase domain containing 2 [So... [more]
RPUSD35.012e-626.92RNA pseudouridine synthase D3 [Source:HGNC Symbol;... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
K07E8.75.129e-1031.03Pseudouridine synthase [Source:UniProtKB/TrEMBL;A... [more]
K07E8.75.309e-1030.82Pseudouridine synthase [Source:UniProtKB/TrEMBL;A... [more]
K07E8.75.524e-1030.82Pseudouridine synthase [Source:UniProtKB/TrEMBL;A... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RluA-12.046e-1033.33gene:FBgn0051719 transcript:FBtr0080040[more]
RluA-12.046e-1033.33gene:FBgn0051719 transcript:FBtr0080039[more]
RluA-12.046e-1033.33gene:FBgn0051719 transcript:FBtr0080041[more]
RluA-21.504e-729.79gene:FBgn0032256 transcript:FBtr0304133[more]
RluA-21.620e-729.79gene:FBgn0032256 transcript:FBtr0337002[more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rpusd45.190e-2732.51RNA pseudouridine synthase D4 [Source:NCBI gene;Ac... [more]
rpusd45.190e-2732.51RNA pseudouridine synthase D4 [Source:NCBI gene;Ac... [more]
rpusd45.190e-2732.51RNA pseudouridine synthase D4 [Source:NCBI gene;Ac... [more]
rpusd42.280e-2632.10RNA pseudouridine synthase D4 [Source:NCBI gene;Ac... [more]
rpusd23.446e-932.14RNA pseudouridine synthase domain containing 2 [So... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ENSXETT00000035080.12.980e-1830.30pep primary_assembly:Xenopus_tropicalis_v9.1:9:819... [more]
RPUSD32.674e-1527.85RNA pseudouridine synthase D3 [Source:NCBI gene;Ac... [more]
RPUSD21.295e-830.22RNA pseudouridylate synthase domain containing 2 [... [more]
RPUSD32.506e-827.17RNA pseudouridine synthase D3 [Source:NCBI gene;Ac... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rpusd46.994e-3333.45RNA pseudouridylate synthase domain containing 4 [... [more]
Rpusd31.441e-1325.08RNA pseudouridylate synthase domain containing 3 [... [more]
Rpusd34.410e-1326.51RNA pseudouridylate synthase domain containing 3 [... [more]
Rpusd36.757e-1325.60RNA pseudouridylate synthase domain containing 3 [... [more]
Rpusd25.371e-1029.35RNA pseudouridylate synthase domain containing 2 [... [more]
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BLAST of RNA pseudouridine synthase D4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q4QQT0|RUSD4_RAT2.329e-3834.01Mitochondrial RNA pseudouridine synthase Rpusd4 OS... [more]
sp|Q96CM3|RUSD4_HUMAN1.271e-3631.82Mitochondrial RNA pseudouridine synthase RPUSD4 OS... [more]
sp|Q9CWX4|RUSD4_MOUSE4.892e-3233.45Mitochondrial RNA pseudouridine synthase Rpusd4 OS... [more]
sp|Q5E9Z1|RUSD4_BOVIN1.526e-3029.97Mitochondrial RNA pseudouridine synthase RPUSD4 OS... [more]
sp|Q28C59|RUSD4_XENTR4.844e-2930.71Mitochondrial RNA pseudouridine synthase rpusd4 OS... [more]
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BLAST of RNA pseudouridine synthase D4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QD542.123e-4336.91RNA pseudouridylate synthase domain-containing pro... [more]
A0A1D1VNM82.468e-4333.01PseudoU_synth_2 domain-containing protein OS=Ramaz... [more]
C3XV248.013e-4134.49PseudoU_synth_2 domain-containing protein OS=Branc... [more]
A0A0L8GZN62.423e-4036.51PseudoU_synth_2 domain-containing protein OS=Octop... [more]
V4AUI78.059e-4034.64PseudoU_synth_2 domain-containing protein OS=Lotti... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpusd43.443e-3136.33RNA pseudouridylate synthase domain-containing pro... [more]
rpusd44.800e-3136.33RNA pseudouridylate synthase domain-containing pro... [more]
rpusd28.043e-930.71RNA pseudouridine synthase domain containing 2 [So... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000009045.14.193e-2830.96pep scaffold:Pmarinus_7.0:GL484265:15078:19154:1 g... [more]
rpusd23.806e-832.62RNA pseudouridine synthase domain containing 2 [So... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
PUS61.700e-1033.10tRNA:pseudouridine synthase; catalyzes the convers... [more]
RIB27.498e-1029.29Bifunctional DRAP deaminase tRNA:pseudouridine syn... [more]
PUS97.684e-1032.37Mitochondrial tRNA:pseudouridine synthase; catalyz... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO475543.866e-3035.69Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO473033.002e-1029.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rpusd44.679e-2931.63mitochondrial RNA pseudouridine synthase rpusd4-li... [more]
rpusd42.612e-2730.64mitochondrial RNA pseudouridine synthase rpusd4-li... [more]
rpusd42.697e-2630.72mitochondrial RNA pseudouridine synthase rpusd4-li... [more]
rpusd46.896e-2329.90mitochondrial RNA pseudouridine synthase rpusd4-li... [more]
rpusd22.186e-1031.43RNA pseudouridine synthase domain containing 2 [So... [more]
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BLAST of RNA pseudouridine synthase D4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000060928.10.000e+099.48SMESG000060928.1[more]
SMESG000051935.11.102e-834.01SMESG000051935.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030321 ID=SMED30030321|Name=RNA pseudouridine synthase D4|organism=Schmidtea mediterranea sexual|type=transcript|length=1268bp
TTGTCACATTTTTATAAATTTGTGCTATACTTTTTTTTATTAAACCTTAG
TAAATTAAAATATTTGAAACATTTTTTTATTTTGTGATATTTGATATTTA
TGGACAAATTAAATATACTTGGATTAAAACCAAATGAATCTTATCAGTTG
GATACGAATTTAGAAAACAACGATAAGAAATCAAACCATACTGAAGACAG
AAGTTCTGCAGCAGAATTTGCTTCTTTTATTAGAGATCAATCTAAAACAA
ATATTCATTTAAATTTGTTTTCTAAATCCCATAACTTAAAACAAAAGGCA
GACTATAAATTTGTTAACGATTTTGACTTTAAAAAATTAAGTAAAGATGA
AATCTCAGTAATTTTAGCTCGGTGTTTTCTTAAATCCAACAACCACGTTA
TAGCTATTAATAAACCTCATGGAATTTCAGTGCAGGGTGGCGACAACGGT
GCATCGATCGAGGAAGTGCTAGAGTTATTGGCGGCAAAACTATCATTGAA
AGAACGTCTGCATATTGTTCATCGTTTGGATAAAGATACGACAGGAGTTT
TACTTCTCGCTCAAAATCCCGATAGTGCTTTTTTATTAAAAGATGCGTTT
AAAGAGAGGCATATTAGAAAAAATTACCTCTGTTTAACCCACGGAATTCC
TCATGAAGAACACGGAATAATCAATTTCAATTTGAAAGAGGCAACACTTG
GTGACCAAATTGTCATACGACCCGATATGAAATCAGTTAAAAAAAACATT
AAAAAGGCTGGTTTCACAAAATCTGAGTCTGGAAAAATATCTACAGCATT
TAATGTGCTGTGTAGCAATTATAATATTTCACTTGTCCAATGTGCAATGA
TTTCCGGTGTGAAGCATCAAATTAGGGCCCACCTGGCGTATGGCTTAGAG
ACGCCAATACTCGGAGATCATAAGTATTCGTGGTTAATAAAAGAAAATAA
ATTTCCGATAATGCCACAACAATTGTCGCCAGTGGCCTTAACTGGATTGA
AACTTACACAAGGAAAGATACGACATTTACCAATCCATTTACACGCATAT
CAGCTAATAATCGGGGAACCTCGGCTGCCGTCGAGTTTATATGATGGAAA
AGCCAATTTGAATTGGAATTCTACCAATAACAAACCGTGCAGATTGCCAG
ATTTCCAAGTTTATGCCAACGTTCCAGATTTCTTTCTTCGGAGCTTGAGA
AGTTTAAAACTTAATGATAGTAAAATTTTCAAGTTTGCTCTAAAACAGAT
ATTATAAAATATAGCATG
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protein sequence of SMED30030321-orf-1

>SMED30030321-orf-1 ID=SMED30030321-orf-1|Name=SMED30030321-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=386bp
MDKLNILGLKPNESYQLDTNLENNDKKSNHTEDRSSAAEFASFIRDQSKT
NIHLNLFSKSHNLKQKADYKFVNDFDFKKLSKDEISVILARCFLKSNNHV
IAINKPHGISVQGGDNGASIEEVLELLAAKLSLKERLHIVHRLDKDTTGV
LLLAQNPDSAFLLKDAFKERHIRKNYLCLTHGIPHEEHGIINFNLKEATL
GDQIVIRPDMKSVKKNIKKAGFTKSESGKISTAFNVLCSNYNISLVQCAM
ISGVKHQIRAHLAYGLETPILGDHKYSWLIKENKFPIMPQQLSPVALTGL
KLTQGKIRHLPIHLHAYQLIIGEPRLPSSLYDGKANLNWNSTNNKPCRLP
DFQVYANVPDFFLRSLRSLKLNDSKIFKFALKQIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR006145PsdUridine_synth_RsuA/RluA
IPR006224PsdUridine_synth_RluC/D_CS
IPR020103PsdUridine_synth_cat_dom_sf
IPR006588Peptide_N_glycanase_PAW_dom
Vocabulary: molecular function
TermDefinition
GO:0009982pseudouridine synthase activity
GO:0003723RNA binding
Vocabulary: biological process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
GO:0006516glycoprotein catabolic process
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.2350.10coord: 90..329
e-value: 4.1E-47
score: 162.5
NoneNo IPR availablePANTHERPTHR21600:SF39RNA PSEUDOURIDYLATE SYNTHASE DOMAIN CONTAINING 4coord: 69..339
NoneNo IPR availablePANTHERPTHR21600RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Bcoord: 69..339
IPR006145Pseudouridine synthase, RsuA/RluAPFAMPF00849PseudoU_synth_2coord: 99..263
e-value: 1.7E-32
score: 112.8
IPR006145Pseudouridine synthase, RsuA/RluACDDcd02869PseudoU_synth_RluCD_likecoord: 100..321
e-value: 8.72908E-46
score: 153.647
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 140..154
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILYSSF55120Pseudouridine synthasecoord: 91..325