SMED30030309
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030309 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15
Homology
BLAST of SMED30030309 vs. Planmine SMEST
Match: SMESG000075212.1 (SMESG000075212.1) HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0 Identity = 389/400 (97.25%), Postives = 394/400 (98.50%), Query Frame = 1 Query: 274 TIKVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSARSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESXXXXXXXXXXXXXXXXXRVKNEIIEETVKNQAKEAEKIVQEKKTELDKKQEAMETMKTKINEKNVKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVDGKSAKPDMNKVACPNCHTLYPQAEIETHIEVCIENEH 1473 + VSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDS +SVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELD+KQEAMETMK KI+EKN+KIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSV GKSAKPDMN VACPNCHTLYPQAEIETHIEVCIENEH Sbjct: 7 SFSVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSGKSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELDRKQEAMETMKIKIDEKNLKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVGGKSAKPDMNTVACPNCHTLYPQAEIETHIEVCIENEH 406
BLAST of SMED30030309 vs. Planmine SMEST
Match: SMESG000075212.1 (SMESG000075212.1) HSP 1 Score: 645.966 bits (1665), Expect = 0.000e+0 Identity = 389/406 (95.81%), Postives = 394/406 (97.04%), Query Frame = 1 Query: 274 TIKVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSARSVNE------ERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESXXXXXXXXXXXXXXXXXRVKNEIIEETVKNQAKEAEKIVQEKKTELDKKQEAMETMKTKINEKNVKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVDGKSAKPDMNKVACPNCHTLYPQAEIETHIEVCIENEH 1473 + VSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDS +SVNE ERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELD+KQEAMETMK KI+EKN+KIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSV GKSAKPDMN VACPNCHTLYPQAEIETHIEVCIENEH Sbjct: 7 SFSVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSGKSVNELSESENERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELDRKQEAMETMKIKIDEKNLKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVGGKSAKPDMNTVACPNCHTLYPQAEIETHIEVCIENEH 412
BLAST of SMED30030309 vs. Planmine SMEST
Match: SMESG000075212.1 (SMESG000075212.1) HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0 Identity = 374/400 (93.50%), Postives = 379/400 (94.75%), Query Frame = 1 Query: 274 TIKVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSARSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESXXXXXXXXXXXXXXXXXRVKNEIIEETVKNQAKEAEKIVQEKKTELDKKQEAMETMKTKINEKNVKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVDGKSAKPDMNKVACPNCHTLYPQAEIETHIEVCIENEH 1473 + VSKHAENLGQEIQSLNQNLEKKEQI KTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDS +SVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELD+KQEAMETMK KI+EKN+KIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSV GKSAKPDMN VACPNCHTLYPQAEIETHIEVCIENEH Sbjct: 7 SFSVSKHAENLGQEIQSLNQNLEKKEQI---------------KTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSGKSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELDRKQEAMETMKIKIDEKNLKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVGGKSAKPDMNTVACPNCHTLYPQAEIETHIEVCIENEH 391
BLAST of SMED30030309 vs. Planmine SMEST
Match: SMESG000075212.1 (SMESG000075212.1) HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0 Identity = 364/400 (91.00%), Postives = 370/400 (92.50%), Query Frame = 1 Query: 274 TIKVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSARSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESXXXXXXXXXXXXXXXXXRVKNEIIEETVKNQAKEAEKIVQEKKTELDKKQEAMETMKTKINEKNVKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVDGKSAKPDMNKVACPNCHTLYPQAEIETHIEVCIENEH 1473 + VSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDS +SVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTK +IESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELD+KQEAMETMK KI+EKN+KIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSV GKSAKPDMN VACPNCHTLYPQAEIETHIEVCIENEH Sbjct: 7 SFSVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSGKSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTK------------------------KIESKNNKLKEMEMESKKQLKKIEELQKEKNNLRVKNEIIEETVKNQAKEAEKIVQEKKTELDRKQEAMETMKIKIDEKNLKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVGGKSAKPDMNTVACPNCHTLYPQAEIETHIEVCIENEH 382
BLAST of SMED30030309 vs. Planmine SMEST
Match: SMESG000075212.1 (SMESG000075212.1) HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0 Identity = 340/400 (85.00%), Postives = 347/400 (86.75%), Query Frame = 1 Query: 274 TIKVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSARSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRKKSKDTKTKAQVKELRNIIEDLKGEVEAKCDEIESKNNKLKEMEMESXXXXXXXXXXXXXXXXXRVKNEIIEETVKNQAKEAEKIVQEKKTELDKKQEAMETMKTKINEKNVKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVDGKSAKPDMNKVACPNCHTLYPQAEIETHIEVCIENEH 1473 + VSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDS +SVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTK IE+L+ E KNN LRVKNEIIEETVKNQAKEAEKIVQEKKTELD+KQEAMETMK KI+EKN+KIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSV GKSAKPDMN VACPNCHTLYPQAEIETHIEVCIENEH Sbjct: 7 SFSVSKHAENLGQEIQSLNQNLEKKEQIIDSKDNVIQKQKNELKTCEDKIKKLDETIRSLQKNPGNITSANVLSRSDSGKSVNEERYKSESQFLNEIYKRSGKKFDNFKECIDYIKNPIDSSSQIRGDLNREWCFARSGNNEMVKELRKEIEILEDEKYAAETKAKSETKKLKKELKDKNEENLELIEEVSELRKKSKDTK-----------IEELQKE----------KNN-------------------------LRVKNEIIEETVKNQAKEAEKIVQEKKTELDRKQEAMETMKIKIDEKNLKIKEYENEIQDHKEKYQELETRANNQNRHHEGKIKILEDQIHELQVRNIENQPKSVGGKSAKPDMNTVACPNCHTLYPQAEIETHIEVCIENEH 360 The following BLAST results are available for this feature:
BLAST of SMED30030309 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030309 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030309 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030309 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030309 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030309 ID=SMED30030309|Name=SMED30030309|organism=Schmidtea mediterranea sexual|type=transcript|length=1625bpback to top protein sequence of SMED30030309-orf-1 >SMED30030309-orf-1 ID=SMED30030309-orf-1|Name=SMED30030309-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=442bp MSDSDEQEAVIQKFKKEKKTLEKKYKDRISELEESIEEKDDTIKVSKHAEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|