SMED30030283

Overview
NameSMED30030283
Smed IDSMED30030283
Length (bp)300
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030283

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30030283h1SMcG0020780 PL06001X1A02smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30030283h1SMcG0020780 Contig2203GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
dorsal ventral margin of the whole animalSMED30030283h1SMcG0020780 Contig2203GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30030283h1SMcG0020780 Contig2203GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30030283h1SMcG0020780 dd_Smed_v4_2138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030283h1SMcG0020780 dd_Smed_v4_2138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030283h1SMcG0020780 dd_Smed_v4_2138_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030283h1SMcG0020780 Contig20101uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030283h1SMcG0020780 Contig20101newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30030283 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006308:20736..21185 -1427
Homology
BLAST of SMED30030283 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 70.0922 bits (170), Expect = 6.606e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  243 LHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 327          

HSP 2 Score: 62.7734 bits (151), Expect = 2.295e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  672 LHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 3 Score: 62.003 bits (149), Expect = 3.998e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  210 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDH 294          

HSP 4 Score: 59.3066 bits (142), Expect = 4.606e-11
Identity = 25/85 (29.41%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H   ++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  639 LHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQH 723          

HSP 5 Score: 56.9954 bits (136), Expect = 2.846e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  265 IETKTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 359          

HSP 6 Score: 55.8398 bits (133), Expect = 7.537e-10
Identity = 25/83 (30.12%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH+  V  LL++  +     +    P+H A + G +++ ELL++      A  ++ + P+H+A  H   DIVKLL+
Sbjct:  507 LHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 589          

HSP 7 Score: 55.4546 bits (132), Expect = 9.338e-10
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH N+VK LL+ N N          P+H A R+GH++ V  L++ +     + +    P+H+A K+G   + +LL++
Sbjct:  474 LHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLE 557          

HSP 8 Score: 54.6842 bits (130), Expect = 1.596e-9
Identity = 29/83 (34.94%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K H++++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  342 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 9 Score: 54.299 bits (129), Expect = 2.547e-9
Identity = 23/85 (27.06%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+ +
Sbjct:  375 LHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQN 459          

HSP 10 Score: 53.9138 bits (128), Expect = 3.616e-9
Identity = 26/87 (29.89%), Postives = 44/87 (50.57%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL     V A  +    P+H A R GH  +V+LL+++  +      +   P+H+A + G  + V  L++
Sbjct:  438 ETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLE 524          

HSP 11 Score: 52.7582 bits (125), Expect = 7.806e-9
Identity = 30/98 (30.61%), Postives = 53/98 (54.08%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K  + A  +    P+H A + G  ++VKLL+++
Sbjct:  397 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLEN 492          

HSP 12 Score: 51.6026 bits (122), Expect = 2.108e-8
Identity = 30/98 (30.61%), Postives = 50/98 (51.02%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V      P+ +A + G+    D++K++ D
Sbjct:  694 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791          

HSP 13 Score: 49.2914 bits (116), Expect = 1.329e-7
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  606 LHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH 690          
BLAST of SMED30030283 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 70.0922 bits (170), Expect = 7.282e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  243 LHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 327          

HSP 2 Score: 62.7734 bits (151), Expect = 2.505e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  672 LHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 3 Score: 62.003 bits (149), Expect = 4.238e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  210 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDH 294          

HSP 4 Score: 58.9214 bits (141), Expect = 5.126e-11
Identity = 25/85 (29.41%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H   ++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  639 LHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQH 723          

HSP 5 Score: 56.6102 bits (135), Expect = 3.137e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  265 IETKTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 359          

HSP 6 Score: 55.4546 bits (132), Expect = 8.148e-10
Identity = 25/83 (30.12%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH+  V  LL++  +     +    P+H A + G +++ ELL++      A  ++ + P+H+A  H   DIVKLL+
Sbjct:  507 LHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 589          

HSP 7 Score: 55.4546 bits (132), Expect = 1.019e-9
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH N+VK LL+ N N          P+H A R+GH++ V  L++ +     + +    P+H+A K+G   + +LL++
Sbjct:  474 LHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLE 557          

HSP 8 Score: 54.6842 bits (130), Expect = 1.776e-9
Identity = 29/83 (34.94%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K H++++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  342 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 9 Score: 53.9138 bits (128), Expect = 2.807e-9
Identity = 23/85 (27.06%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+ +
Sbjct:  375 LHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQN 459          

HSP 10 Score: 53.5286 bits (127), Expect = 3.909e-9
Identity = 26/87 (29.89%), Postives = 44/87 (50.57%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL     V A  +    P+H A R GH  +V+LL+++  +      +   P+H+A + G  + V  L++
Sbjct:  438 ETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLE 524          

HSP 11 Score: 52.7582 bits (125), Expect = 8.521e-9
Identity = 30/98 (30.61%), Postives = 53/98 (54.08%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K  + A  +    P+H A + G  ++VKLL+++
Sbjct:  397 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLEN 492          

HSP 12 Score: 51.6026 bits (122), Expect = 2.347e-8
Identity = 30/98 (30.61%), Postives = 50/98 (51.02%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V      P+ +A + G+    D++K++ D
Sbjct:  694 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791          

HSP 13 Score: 49.2914 bits (116), Expect = 1.451e-7
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  606 LHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH 690          
BLAST of SMED30030283 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 69.707 bits (169), Expect = 8.028e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  276 LHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 360          

HSP 2 Score: 62.7734 bits (151), Expect = 2.789e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  705 LHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 787          

HSP 3 Score: 62.003 bits (149), Expect = 4.627e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  243 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDH 327          

HSP 4 Score: 58.9214 bits (141), Expect = 5.436e-11
Identity = 25/85 (29.41%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H   ++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  672 LHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQH 756          

HSP 5 Score: 56.6102 bits (135), Expect = 3.392e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  298 IETKTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 392          

HSP 6 Score: 55.4546 bits (132), Expect = 8.896e-10
Identity = 25/83 (30.12%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH+  V  LL++  +     +    P+H A + G +++ ELL++      A  ++ + P+H+A  H   DIVKLL+
Sbjct:  540 LHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 622          

HSP 7 Score: 55.0694 bits (131), Expect = 1.060e-9
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH N+VK LL+ N N          P+H A R+GH++ V  L++ +     + +    P+H+A K+G   + +LL++
Sbjct:  507 LHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLE 590          

HSP 8 Score: 54.6842 bits (130), Expect = 1.920e-9
Identity = 29/83 (34.94%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K H++++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  375 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 457          

HSP 9 Score: 53.9138 bits (128), Expect = 3.095e-9
Identity = 23/85 (27.06%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+ +
Sbjct:  408 LHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQN 492          

HSP 10 Score: 53.5286 bits (127), Expect = 4.186e-9
Identity = 26/87 (29.89%), Postives = 44/87 (50.57%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL     V A  +    P+H A R GH  +V+LL+++  +      +   P+H+A + G  + V  L++
Sbjct:  471 ETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLE 557          

HSP 11 Score: 52.7582 bits (125), Expect = 9.214e-9
Identity = 30/98 (30.61%), Postives = 53/98 (54.08%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K  + A  +    P+H A + G  ++VKLL+++
Sbjct:  430 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLEN 525          

HSP 12 Score: 51.2174 bits (121), Expect = 2.613e-8
Identity = 30/98 (30.61%), Postives = 50/98 (51.02%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V      P+ +A + G+    D++K++ D
Sbjct:  727 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 824          

HSP 13 Score: 49.2914 bits (116), Expect = 1.524e-7
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  639 LHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH 723          
BLAST of SMED30030283 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 67.781 bits (164), Expect = 4.136e-14
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R+    +H A K+G+ N+VK LLD+   ++A  R  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  261 VDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 356          

HSP 2 Score: 66.2402 bits (160), Expect = 1.539e-13
Identity = 34/86 (39.53%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  N  A       P+H A +K  IK++ELL+ H   I+AV  S + PIH+A   G  +IV  L+ H
Sbjct:  370 ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHH 455          

HSP 3 Score: 63.929 bits (154), Expect = 8.214e-13
Identity = 31/97 (31.96%), Postives = 55/97 (56.70%), Query Frame = 1
Query:   19 DKVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +  DV  +S    +H A   G++NV   LL++   V+ T R  I P+H A+++G+  +V+LL+D    I+A  R  + P+H   + G   +V++L+D
Sbjct:  226 NNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 322          

HSP 4 Score: 63.929 bits (154), Expect = 1.028e-12
Identity = 25/86 (29.07%), Postives = 50/86 (58.14%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A ++GH+++V  LL +N NV  + +  + P+H A ++  + + E+L++    ++A  +    P+H+ C +G   IV  L+ H
Sbjct:  667 SVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQH 752          

HSP 5 Score: 58.151 bits (139), Expect = 1.080e-10
Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV++L+     VEA  +    P+H + R G   IV+ L+       A   S   P+HL+ + G  D+   L+DH
Sbjct:  467 ETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDH 554          

HSP 6 Score: 55.8398 bits (133), Expect = 5.878e-10
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  N  V+      +  +H A   GH K+ ++L+D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  338 LHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKH 422          

HSP 7 Score: 55.4546 bits (132), Expect = 9.291e-10
Identity = 27/96 (28.12%), Postives = 49/96 (51.04%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            K+D   R     +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + K+L+D
Sbjct:  293 KIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD 388          

HSP 8 Score: 54.299 bits (129), Expect = 2.390e-9
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++  +NV + L++Q  +V+A  +    P+H     G+IKIV  L+ H   + A  ++   P+H A + G   I+ +L+ +
Sbjct:  701 LHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 785          

HSP 9 Score: 54.299 bits (129), Expect = 2.461e-9
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH +V  FLLD   ++  T +    P+H A + G +++  LL+      +A  +S + P+H+A  +    +  LL+D 
Sbjct:  536 LHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 620          

HSP 10 Score: 54.299 bits (129), Expect = 2.634e-9
Identity = 24/86 (27.91%), Postives = 51/86 (59.30%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  LL +  NV+A  +     +H A+  G  ++V++L+ +  ++ A  ++   P+++A +    ++VK L+D+
Sbjct:   77 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDN 162          

HSP 11 Score: 52.7582 bits (125), Expect = 8.076e-9
Identity = 27/96 (28.12%), Postives = 48/96 (50.00%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +EA  K D T   +H + + G  ++V+ LL Q  +  A       P+H + R+GH  +   L+DH   +    +    P+H+A K+G  ++  LL+
Sbjct:  492 VEAKAKDDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 585          

HSP 12 Score: 51.9878 bits (123), Expect = 1.743e-8
Identity = 21/85 (24.71%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  +++   LL+   +  A  R  I  +H A ++GH+ +V LL+    ++   ++S + P+HLA +    ++ ++L++ 
Sbjct:  635 LHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQ 719          

HSP 13 Score: 51.6026 bits (122), Expect = 1.940e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G+I +  LL++    ++   R+ + P+H+A K G  ++VKLL+D
Sbjct:  205 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLD 289          

HSP 14 Score: 51.2174 bits (121), Expect = 2.404e-8
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  A  +    P+H A +K  + I   L+++  D  AV R  +  +HLA + G  D+V LL+
Sbjct:  602 LHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 684          

HSP 15 Score: 47.3654 bits (111), Expect = 5.927e-7
Identity = 30/99 (30.30%), Postives = 51/99 (51.52%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   IH A   GH+N+V  L+    +   T +RG+   +H A R G  ++V  L+     +EA  +    P+H++ + G  DIV+ L+  
Sbjct:  426 IQAVTESGLT--PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGE-TALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ 521          

HSP 16 Score: 46.2098 bits (108), Expect = 1.452e-6
Identity = 22/86 (25.58%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   G   VVK L+    NV A  +    P++ A ++ H+++V+ L+D+             P+ +A + G   +V LL+++
Sbjct:  110 ALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN 195          

HSP 17 Score: 46.2098 bits (108), Expect = 1.664e-6
Identity = 24/85 (28.24%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G L V   LL ++ + +A  +  + P+H A    + K+  LL+D      A  ++   P+H+A K    DI   L+++
Sbjct:  569 LHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEY 653          
BLAST of SMED30030283 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 67.3958 bits (163), Expect = 5.050e-14
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R+    +H A K+G+ N+VK LLD+   ++A  R  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  255 VDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 350          

HSP 2 Score: 65.855 bits (159), Expect = 1.954e-13
Identity = 34/86 (39.53%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  N  A       P+H A +K  IK++ELL+ H   I+AV  S + PIH+A   G  +IV  L+ H
Sbjct:  364 ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHH 449          

HSP 3 Score: 63.929 bits (154), Expect = 1.033e-12
Identity = 31/97 (31.96%), Postives = 55/97 (56.70%), Query Frame = 1
Query:   19 DKVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +  DV  +S    +H A   G++NV   LL++   V+ T R  I P+H A+++G+  +V+LL+D    I+A  R  + P+H   + G   +V++L+D
Sbjct:  220 NNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 316          

HSP 4 Score: 63.5438 bits (153), Expect = 1.172e-12
Identity = 25/86 (29.07%), Postives = 50/86 (58.14%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A ++GH+++V  LL +N NV  + +  + P+H A ++  + + E+L++    ++A  +    P+H+ C +G   IV  L+ H
Sbjct:  661 SVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQH 746          

HSP 5 Score: 57.7658 bits (138), Expect = 1.268e-10
Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV++L+     VEA  +    P+H + R G   IV+ L+       A   S   P+HL+ + G  D+   L+DH
Sbjct:  461 ETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDH 548          

HSP 6 Score: 55.8398 bits (133), Expect = 6.836e-10
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  N  V+      +  +H A   GH K+ ++L+D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  332 LHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKH 416          

HSP 7 Score: 55.0694 bits (131), Expect = 1.091e-9
Identity = 27/96 (28.12%), Postives = 49/96 (51.04%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            K+D   R     +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + K+L+D
Sbjct:  287 KIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD 382          

HSP 8 Score: 53.9138 bits (128), Expect = 2.806e-9
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++  +NV + L++Q  +V+A  +    P+H     G+IKIV  L+ H   + A  ++   P+H A + G   I+ +L+ +
Sbjct:  695 LHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 779          

HSP 9 Score: 53.9138 bits (128), Expect = 2.834e-9
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH +V  FLLD   ++  T +    P+H A + G +++  LL+      +A  +S + P+H+A  +    +  LL+D 
Sbjct:  530 LHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 614          

HSP 10 Score: 53.9138 bits (128), Expect = 3.093e-9
Identity = 24/86 (27.91%), Postives = 51/86 (59.30%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  LL +  NV+A  +     +H A+  G  ++V++L+ +  ++ A  ++   P+++A +    ++VK L+D+
Sbjct:   71 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDN 156          

HSP 11 Score: 52.373 bits (124), Expect = 9.669e-9
Identity = 27/96 (28.12%), Postives = 48/96 (50.00%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +EA  K D T   +H + + G  ++V+ LL Q  +  A       P+H + R+GH  +   L+DH   +    +    P+H+A K+G  ++  LL+
Sbjct:  486 VEAKAKDDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 579          

HSP 12 Score: 51.6026 bits (122), Expect = 1.988e-8
Identity = 21/85 (24.71%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  +++   LL+   +  A  R  I  +H A ++GH+ +V LL+    ++   ++S + P+HLA +    ++ ++L++ 
Sbjct:  629 LHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQ 713          

HSP 13 Score: 51.6026 bits (122), Expect = 2.235e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G+I +  LL++    ++   R+ + P+H+A K G  ++VKLL+D
Sbjct:  199 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLD 283          

HSP 14 Score: 51.2174 bits (121), Expect = 2.851e-8
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  A  +    P+H A +K  + I   L+++  D  AV R  +  +HLA + G  D+V LL+
Sbjct:  596 LHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 678          

HSP 15 Score: 47.3654 bits (111), Expect = 6.893e-7
Identity = 30/99 (30.30%), Postives = 51/99 (51.52%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   IH A   GH+N+V  L+    +   T +RG+   +H A R G  ++V  L+     +EA  +    P+H++ + G  DIV+ L+  
Sbjct:  420 IQAVTESGLT--PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETA-LHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ 515          

HSP 16 Score: 46.2098 bits (108), Expect = 1.673e-6
Identity = 22/86 (25.58%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   G   VVK L+    NV A  +    P++ A ++ H+++V+ L+D+             P+ +A + G   +V LL+++
Sbjct:  104 ALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN 189          

HSP 17 Score: 45.8246 bits (107), Expect = 1.974e-6
Identity = 24/85 (28.24%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G L V   LL ++ + +A  +  + P+H A    + K+  LL+D      A  ++   P+H+A K    DI   L+++
Sbjct:  563 LHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEY 647          
BLAST of SMED30030283 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 61.2326 bits (147), Expect = 2.896e-12
Identity = 31/96 (32.29%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   22 KVDVTERSI----HFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            KVD   R +    H A + G+ ++V  LL    N  AT R    P+H A ++G  ++  +L+DH  D   + +    P+HLA K+G  ++V+LL++
Sbjct:  483 KVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 2 Score: 60.4622 bits (145), Expect = 5.726e-12
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DVT +S    +H A   GH NV + LL++  NV    R  I P+H AT+ G   +  LL+     I++  + L+ P+H A + G   +V LL+
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 3 Score: 58.5362 bits (140), Expect = 2.912e-11
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD V V   + +H A   GH+ V K LLD++ +  +       P+H A +K  IK+VELL+ ++  IEA   S + P+H+A   G  +IV  L+  
Sbjct:  352 VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 4 Score: 55.8398 bits (133), Expect = 2.295e-10
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IEA  +  +T   +H A   G +N+V +LL Q  N +  T+RG+  P+H A R     +V +LI +   ++A  R L  P+H+A + G  DIV LL+
Sbjct:  418 IEATTESGLT--PLHVAAFMGAINIVIYLLQQGANPDVETVRGE-TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511          

HSP 5 Score: 55.4546 bits (132), Expect = 3.772e-10
Identity = 28/88 (31.82%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    +VV+ L+     V+A  R    P+H A+R G+  IV LL+    +  A  R    P+H+A K G  ++  +L+DH
Sbjct:  459 ETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH 546          

HSP 6 Score: 51.6026 bits (122), Expect = 7.802e-9
Identity = 24/84 (28.57%), Postives = 42/84 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  VV  L+ Q   + A  +  + P+H A +  H+     L+ H+  ++ V    + P+H+A   G   + KLL+D
Sbjct:  297 LHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLD 380          

HSP 7 Score: 50.8322 bits (120), Expect = 1.270e-8
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+   LL +   +++  +  + P+H A R GH ++V+LL+     I A  ++ + P+H+A +    D  + L+ H
Sbjct:  264 LHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYH 348          

HSP 8 Score: 50.0618 bits (118), Expect = 2.963e-8
Identity = 26/94 (27.66%), Postives = 46/94 (48.94%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            E   K +     +H AC  G LN+VKFL++   +V    R    P+H+A ++GH   V  L+++         +   P+ +A + G+  +V+ L
Sbjct:  714 EINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 49.2914 bits (116), Expect = 4.375e-8
Identity = 22/85 (25.88%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G   V   LLD N +     +    P+H A++ G++++V LL++    ++   ++ V P+H+A  +    +  LL+++
Sbjct:  528 LHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 10 Score: 49.2914 bits (116), Expect = 4.823e-8
Identity = 24/86 (27.91%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A K+GH  VV+ L+ +   V+A  R     +H A+  G   IV +L+++  ++     +   P+++A +    ++VK L+ H
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKH 154          

HSP 11 Score: 49.2914 bits (116), Expect = 4.966e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      + P+H A   GH+++ +LL+D   D  +   +   P+H+ACK     +V+LL+ +
Sbjct:  330 LHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKY 414          

HSP 12 Score: 48.521 bits (114), Expect = 8.909e-8
Identity = 28/99 (28.28%), Postives = 48/99 (48.48%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            DIE  ++V      +H A    +  V   LL+   + +A  +    P+H A +K  ++I   L+  K D  A  R+   P+HL+ + G  +I  LLI++
Sbjct:  584 DIEGKNQVT----PLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIEN 678          

HSP 13 Score: 47.3654 bits (111), Expect = 2.314e-7
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 1
Query:   19 DKVDVTERS---IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DK  +T++    +H A K G+L VV+ LL++   V+   + ++ P+H A    + K+  LL+++    +A  ++   P+H+A K    +I   L+
Sbjct:  549 DKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 14 Score: 46.595 bits (109), Expect = 4.806e-7
Identity = 18/85 (21.18%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH  +   L++   +V A     +  +H   ++ H+ + ++L ++  +I +   +   P+H+AC  G  ++VK L+++
Sbjct:  660 LHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 15 Score: 45.0542 bits (105), Expect = 1.532e-6
Identity = 22/84 (26.19%), Postives = 40/84 (47.62%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A K+        LL    N + T +    P+H A   GH  + +LL++   ++    R  + P+H+A K G  ++  LL+
Sbjct:  197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLL 280          

HSP 16 Score: 44.2838 bits (103), Expect = 2.829e-6
Identity = 18/86 (20.93%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H   ++ H+ V + L +    + +       P+H A   G + +V+ L+++  D+    R+   P+H A + G  + V+ L+++
Sbjct:  692 AMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLEN 777          
BLAST of SMED30030283 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 61.2326 bits (147), Expect = 2.896e-12
Identity = 31/96 (32.29%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   22 KVDVTERSI----HFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            KVD   R +    H A + G+ ++V  LL    N  AT R    P+H A ++G  ++  +L+DH  D   + +    P+HLA K+G  ++V+LL++
Sbjct:  483 KVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 2 Score: 60.4622 bits (145), Expect = 5.726e-12
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DVT +S    +H A   GH NV + LL++  NV    R  I P+H AT+ G   +  LL+     I++  + L+ P+H A + G   +V LL+
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 3 Score: 58.5362 bits (140), Expect = 2.912e-11
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD V V   + +H A   GH+ V K LLD++ +  +       P+H A +K  IK+VELL+ ++  IEA   S + P+H+A   G  +IV  L+  
Sbjct:  352 VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 4 Score: 55.8398 bits (133), Expect = 2.295e-10
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IEA  +  +T   +H A   G +N+V +LL Q  N +  T+RG+  P+H A R     +V +LI +   ++A  R L  P+H+A + G  DIV LL+
Sbjct:  418 IEATTESGLT--PLHVAAFMGAINIVIYLLQQGANPDVETVRGE-TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511          

HSP 5 Score: 55.4546 bits (132), Expect = 3.772e-10
Identity = 28/88 (31.82%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    +VV+ L+     V+A  R    P+H A+R G+  IV LL+    +  A  R    P+H+A K G  ++  +L+DH
Sbjct:  459 ETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH 546          

HSP 6 Score: 51.6026 bits (122), Expect = 7.802e-9
Identity = 24/84 (28.57%), Postives = 42/84 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  VV  L+ Q   + A  +  + P+H A +  H+     L+ H+  ++ V    + P+H+A   G   + KLL+D
Sbjct:  297 LHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLD 380          

HSP 7 Score: 50.8322 bits (120), Expect = 1.270e-8
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+   LL +   +++  +  + P+H A R GH ++V+LL+     I A  ++ + P+H+A +    D  + L+ H
Sbjct:  264 LHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYH 348          

HSP 8 Score: 50.0618 bits (118), Expect = 2.963e-8
Identity = 26/94 (27.66%), Postives = 46/94 (48.94%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            E   K +     +H AC  G LN+VKFL++   +V    R    P+H+A ++GH   V  L+++         +   P+ +A + G+  +V+ L
Sbjct:  714 EINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 49.2914 bits (116), Expect = 4.375e-8
Identity = 22/85 (25.88%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G   V   LLD N +     +    P+H A++ G++++V LL++    ++   ++ V P+H+A  +    +  LL+++
Sbjct:  528 LHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 10 Score: 49.2914 bits (116), Expect = 4.823e-8
Identity = 24/86 (27.91%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A K+GH  VV+ L+ +   V+A  R     +H A+  G   IV +L+++  ++     +   P+++A +    ++VK L+ H
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKH 154          

HSP 11 Score: 49.2914 bits (116), Expect = 4.966e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      + P+H A   GH+++ +LL+D   D  +   +   P+H+ACK     +V+LL+ +
Sbjct:  330 LHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKY 414          

HSP 12 Score: 48.521 bits (114), Expect = 8.909e-8
Identity = 28/99 (28.28%), Postives = 48/99 (48.48%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            DIE  ++V      +H A    +  V   LL+   + +A  +    P+H A +K  ++I   L+  K D  A  R+   P+HL+ + G  +I  LLI++
Sbjct:  584 DIEGKNQVT----PLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIEN 678          

HSP 13 Score: 47.3654 bits (111), Expect = 2.314e-7
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 1
Query:   19 DKVDVTERS---IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DK  +T++    +H A K G+L VV+ LL++   V+   + ++ P+H A    + K+  LL+++    +A  ++   P+H+A K    +I   L+
Sbjct:  549 DKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 14 Score: 46.595 bits (109), Expect = 4.806e-7
Identity = 18/85 (21.18%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH  +   L++   +V A     +  +H   ++ H+ + ++L ++  +I +   +   P+H+AC  G  ++VK L+++
Sbjct:  660 LHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 15 Score: 45.0542 bits (105), Expect = 1.532e-6
Identity = 22/84 (26.19%), Postives = 40/84 (47.62%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A K+        LL    N + T +    P+H A   GH  + +LL++   ++    R  + P+H+A K G  ++  LL+
Sbjct:  197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLL 280          

HSP 16 Score: 44.2838 bits (103), Expect = 2.829e-6
Identity = 18/86 (20.93%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H   ++ H+ V + L +    + +       P+H A   G + +V+ L+++  D+    R+   P+H A + G  + V+ L+++
Sbjct:  692 AMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLEN 777          
BLAST of SMED30030283 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 61.2326 bits (147), Expect = 3.225e-12
Identity = 31/96 (32.29%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   22 KVDVTERSI----HFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            KVD   R +    H A + G+ ++V  LL    N  AT R    P+H A ++G  ++  +L+DH  D   + +    P+HLA K+G  ++V+LL++
Sbjct:  483 KVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 2 Score: 60.4622 bits (145), Expect = 6.192e-12
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DVT +S    +H A   GH NV + LL++  NV    R  I P+H AT+ G   +  LL+     I++  + L+ P+H A + G   +V LL+
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 3 Score: 58.5362 bits (140), Expect = 3.242e-11
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD V V   + +H A   GH+ V K LLD++ +  +       P+H A +K  IK+VELL+ ++  IEA   S + P+H+A   G  +IV  L+  
Sbjct:  352 VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 4 Score: 55.8398 bits (133), Expect = 2.556e-10
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IEA  +  +T   +H A   G +N+V +LL Q  N +  T+RG+  P+H A R     +V +LI +   ++A  R L  P+H+A + G  DIV LL+
Sbjct:  418 IEATTESGLT--PLHVAAFMGAINIVIYLLQQGANPDVETVRGE-TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511          

HSP 5 Score: 55.0694 bits (131), Expect = 4.200e-10
Identity = 28/88 (31.82%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    +VV+ L+     V+A  R    P+H A+R G+  IV LL+    +  A  R    P+H+A K G  ++  +L+DH
Sbjct:  459 ETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH 546          

HSP 6 Score: 51.6026 bits (122), Expect = 8.520e-9
Identity = 24/84 (28.57%), Postives = 42/84 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  VV  L+ Q   + A  +  + P+H A +  H+     L+ H+  ++ V    + P+H+A   G   + KLL+D
Sbjct:  297 LHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLD 380          

HSP 7 Score: 50.8322 bits (120), Expect = 1.400e-8
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+   LL +   +++  +  + P+H A R GH ++V+LL+     I A  ++ + P+H+A +    D  + L+ H
Sbjct:  264 LHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYH 348          

HSP 8 Score: 49.6766 bits (117), Expect = 3.300e-8
Identity = 26/94 (27.66%), Postives = 46/94 (48.94%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            E   K +     +H AC  G LN+VKFL++   +V    R    P+H+A ++GH   V  L+++         +   P+ +A + G+  +V+ L
Sbjct:  714 EINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 49.2914 bits (116), Expect = 4.778e-8
Identity = 22/85 (25.88%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G   V   LLD N +     +    P+H A++ G++++V LL++    ++   ++ V P+H+A  +    +  LL+++
Sbjct:  528 LHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 10 Score: 49.2914 bits (116), Expect = 5.370e-8
Identity = 24/86 (27.91%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A K+GH  VV+ L+ +   V+A  R     +H A+  G   IV +L+++  ++     +   P+++A +    ++VK L+ H
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKH 154          

HSP 11 Score: 49.2914 bits (116), Expect = 5.476e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      + P+H A   GH+++ +LL+D   D  +   +   P+H+ACK     +V+LL+ +
Sbjct:  330 LHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKY 414          

HSP 12 Score: 48.521 bits (114), Expect = 9.921e-8
Identity = 28/99 (28.28%), Postives = 48/99 (48.48%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            DIE  ++V      +H A    +  V   LL+   + +A  +    P+H A +K  ++I   L+  K D  A  R+   P+HL+ + G  +I  LLI++
Sbjct:  584 DIEGKNQVT----PLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIEN 678          

HSP 13 Score: 47.3654 bits (111), Expect = 2.577e-7
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 1
Query:   19 DKVDVTERS---IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DK  +T++    +H A K G+L VV+ LL++   V+   + ++ P+H A    + K+  LL+++    +A  ++   P+H+A K    +I   L+
Sbjct:  549 DKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 14 Score: 46.2098 bits (108), Expect = 5.351e-7
Identity = 18/85 (21.18%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH  +   L++   +V A     +  +H   ++ H+ + ++L ++  +I +   +   P+H+AC  G  ++VK L+++
Sbjct:  660 LHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 15 Score: 45.0542 bits (105), Expect = 1.673e-6
Identity = 22/84 (26.19%), Postives = 40/84 (47.62%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A K+        LL    N + T +    P+H A   GH  + +LL++   ++    R  + P+H+A K G  ++  LL+
Sbjct:  197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLL 280          

HSP 16 Score: 44.2838 bits (103), Expect = 3.090e-6
Identity = 18/86 (20.93%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H   ++ H+ V + L +    + +       P+H A   G + +V+ L+++  D+    R+   P+H A + G  + V+ L+++
Sbjct:  692 AMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLEN 777          
BLAST of SMED30030283 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 61.2326 bits (147), Expect = 3.225e-12
Identity = 31/96 (32.29%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   22 KVDVTERSI----HFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            KVD   R +    H A + G+ ++V  LL    N  AT R    P+H A ++G  ++  +L+DH  D   + +    P+HLA K+G  ++V+LL++
Sbjct:  483 KVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 2 Score: 60.4622 bits (145), Expect = 6.192e-12
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DVT +S    +H A   GH NV + LL++  NV    R  I P+H AT+ G   +  LL+     I++  + L+ P+H A + G   +V LL+
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 3 Score: 58.5362 bits (140), Expect = 3.242e-11
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD V V   + +H A   GH+ V K LLD++ +  +       P+H A +K  IK+VELL+ ++  IEA   S + P+H+A   G  +IV  L+  
Sbjct:  352 VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 4 Score: 55.8398 bits (133), Expect = 2.556e-10
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IEA  +  +T   +H A   G +N+V +LL Q  N +  T+RG+  P+H A R     +V +LI +   ++A  R L  P+H+A + G  DIV LL+
Sbjct:  418 IEATTESGLT--PLHVAAFMGAINIVIYLLQQGANPDVETVRGE-TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511          

HSP 5 Score: 55.0694 bits (131), Expect = 4.200e-10
Identity = 28/88 (31.82%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    +VV+ L+     V+A  R    P+H A+R G+  IV LL+    +  A  R    P+H+A K G  ++  +L+DH
Sbjct:  459 ETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH 546          

HSP 6 Score: 51.6026 bits (122), Expect = 8.520e-9
Identity = 24/84 (28.57%), Postives = 42/84 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  VV  L+ Q   + A  +  + P+H A +  H+     L+ H+  ++ V    + P+H+A   G   + KLL+D
Sbjct:  297 LHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLD 380          

HSP 7 Score: 50.8322 bits (120), Expect = 1.400e-8
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+   LL +   +++  +  + P+H A R GH ++V+LL+     I A  ++ + P+H+A +    D  + L+ H
Sbjct:  264 LHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYH 348          

HSP 8 Score: 49.6766 bits (117), Expect = 3.300e-8
Identity = 26/94 (27.66%), Postives = 46/94 (48.94%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            E   K +     +H AC  G LN+VKFL++   +V    R    P+H+A ++GH   V  L+++         +   P+ +A + G+  +V+ L
Sbjct:  714 EINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 49.2914 bits (116), Expect = 4.778e-8
Identity = 22/85 (25.88%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G   V   LLD N +     +    P+H A++ G++++V LL++    ++   ++ V P+H+A  +    +  LL+++
Sbjct:  528 LHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 10 Score: 49.2914 bits (116), Expect = 5.370e-8
Identity = 24/86 (27.91%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A K+GH  VV+ L+ +   V+A  R     +H A+  G   IV +L+++  ++     +   P+++A +    ++VK L+ H
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKH 154          

HSP 11 Score: 49.2914 bits (116), Expect = 5.476e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      + P+H A   GH+++ +LL+D   D  +   +   P+H+ACK     +V+LL+ +
Sbjct:  330 LHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKY 414          

HSP 12 Score: 48.521 bits (114), Expect = 9.921e-8
Identity = 28/99 (28.28%), Postives = 48/99 (48.48%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            DIE  ++V      +H A    +  V   LL+   + +A  +    P+H A +K  ++I   L+  K D  A  R+   P+HL+ + G  +I  LLI++
Sbjct:  584 DIEGKNQVT----PLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIEN 678          

HSP 13 Score: 47.3654 bits (111), Expect = 2.577e-7
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 1
Query:   19 DKVDVTERS---IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DK  +T++    +H A K G+L VV+ LL++   V+   + ++ P+H A    + K+  LL+++    +A  ++   P+H+A K    +I   L+
Sbjct:  549 DKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 14 Score: 46.2098 bits (108), Expect = 5.351e-7
Identity = 18/85 (21.18%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH  +   L++   +V A     +  +H   ++ H+ + ++L ++  +I +   +   P+H+AC  G  ++VK L+++
Sbjct:  660 LHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 15 Score: 45.0542 bits (105), Expect = 1.673e-6
Identity = 22/84 (26.19%), Postives = 40/84 (47.62%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A K+        LL    N + T +    P+H A   GH  + +LL++   ++    R  + P+H+A K G  ++  LL+
Sbjct:  197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLL 280          

HSP 16 Score: 44.2838 bits (103), Expect = 3.090e-6
Identity = 18/86 (20.93%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H   ++ H+ V + L +    + +       P+H A   G + +V+ L+++  D+    R+   P+H A + G  + V+ L+++
Sbjct:  692 AMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLEN 777          
BLAST of SMED30030283 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 60.8474 bits (146), Expect = 4.071e-12
Identity = 31/96 (32.29%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   22 KVDVTERSI----HFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            KVD   R +    H A + G+ ++V  LL    N  AT R    P+H A ++G  ++  +L+DH  D   + +    P+HLA K+G  ++V+LL++
Sbjct:  483 KVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 2 Score: 60.077 bits (144), Expect = 7.971e-12
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DVT +S    +H A   GH NV + LL++  NV    R  I P+H AT+ G   +  LL+     I++  + L+ P+H A + G   +V LL+
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 3 Score: 58.151 bits (139), Expect = 3.936e-11
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD V V   + +H A   GH+ V K LLD++ +  +       P+H A +K  IK+VELL+ ++  IEA   S + P+H+A   G  +IV  L+  
Sbjct:  352 VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 4 Score: 55.4546 bits (132), Expect = 3.258e-10
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IEA  +  +T   +H A   G +N+V +LL Q  N +  T+RG+  P+H A R     +V +LI +   ++A  R L  P+H+A + G  DIV LL+
Sbjct:  418 IEATTESGLT--PLHVAAFMGAINIVIYLLQQGANPDVETVRGE-TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511          

HSP 5 Score: 55.0694 bits (131), Expect = 5.100e-10
Identity = 28/88 (31.82%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    +VV+ L+     V+A  R    P+H A+R G+  IV LL+    +  A  R    P+H+A K G  ++  +L+DH
Sbjct:  459 ETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH 546          

HSP 6 Score: 51.2174 bits (121), Expect = 9.853e-9
Identity = 24/84 (28.57%), Postives = 42/84 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  VV  L+ Q   + A  +  + P+H A +  H+     L+ H+  ++ V    + P+H+A   G   + KLL+D
Sbjct:  297 LHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLD 380          

HSP 7 Score: 50.8322 bits (120), Expect = 1.651e-8
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+   LL +   +++  +  + P+H A R GH ++V+LL+     I A  ++ + P+H+A +    D  + L+ H
Sbjct:  264 LHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYH 348          

HSP 8 Score: 49.6766 bits (117), Expect = 3.853e-8
Identity = 26/94 (27.66%), Postives = 46/94 (48.94%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            E   K +     +H AC  G LN+VKFL++   +V    R    P+H+A ++GH   V  L+++         +   P+ +A + G+  +V+ L
Sbjct:  714 EINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 9 Score: 49.2914 bits (116), Expect = 5.634e-8
Identity = 22/85 (25.88%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G   V   LLD N +     +    P+H A++ G++++V LL++    ++   ++ V P+H+A  +    +  LL+++
Sbjct:  528 LHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 10 Score: 48.9062 bits (115), Expect = 6.151e-8
Identity = 24/86 (27.91%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A K+GH  VV+ L+ +   V+A  R     +H A+  G   IV +L+++  ++     +   P+++A +    ++VK L+ H
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKH 154          

HSP 11 Score: 48.9062 bits (115), Expect = 6.333e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      + P+H A   GH+++ +LL+D   D  +   +   P+H+ACK     +V+LL+ +
Sbjct:  330 LHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKY 414          

HSP 12 Score: 48.1358 bits (113), Expect = 1.205e-7
Identity = 28/99 (28.28%), Postives = 48/99 (48.48%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            DIE  ++V      +H A    +  V   LL+   + +A  +    P+H A +K  ++I   L+  K D  A  R+   P+HL+ + G  +I  LLI++
Sbjct:  584 DIEGKNQVT----PLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIEN 678          

HSP 13 Score: 46.9802 bits (110), Expect = 3.010e-7
Identity = 26/95 (27.37%), Postives = 51/95 (53.68%), Query Frame = 1
Query:   19 DKVDVTERS---IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DK  +T++    +H A K G+L VV+ LL++   V+   + ++ P+H A    + K+  LL+++    +A  ++   P+H+A K    +I   L+
Sbjct:  549 DKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 14 Score: 46.2098 bits (108), Expect = 5.952e-7
Identity = 18/85 (21.18%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH  +   L++   +V A     +  +H   ++ H+ + ++L ++  +I +   +   P+H+AC  G  ++VK L+++
Sbjct:  660 LHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 15 Score: 44.669 bits (104), Expect = 1.972e-6
Identity = 22/84 (26.19%), Postives = 40/84 (47.62%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A K+        LL    N + T +    P+H A   GH  + +LL++   ++    R  + P+H+A K G  ++  LL+
Sbjct:  197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLL 280          

HSP 16 Score: 43.8986 bits (102), Expect = 3.504e-6
Identity = 18/86 (20.93%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H   ++ H+ V + L +    + +       P+H A   G + +V+ L+++  D+    R+   P+H A + G  + V+ L+++
Sbjct:  692 AMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLEN 777          
BLAST of SMED30030283 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303117)

HSP 1 Score: 63.929 bits (154), Expect = 3.932e-13
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTE-RSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD+V V    ++H A   GH+ V K LLD+N +  A       P+H A +K  +K+VELL+ H   I A   S + P+H+A   G  +IV  L+ H
Sbjct:  330 VDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH 425          

HSP 2 Score: 61.6178 bits (148), Expect = 3.009e-12
Identity = 28/85 (32.94%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+V  LL++  N+EA  R  + P+H A R GH ++V++L++    I A  ++ + P+H+A +    D  ++L+ H
Sbjct:  242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH 326          

HSP 3 Score: 59.3066 bits (142), Expect = 2.215e-11
Identity = 27/96 (28.12%), Postives = 53/96 (55.21%), Query Frame = 1
Query:   22 KVDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +VD T +    ++H A K+G   V   L++    ++A  +    P+H   + GHIK+ +LL+  + D++A  ++ V P+H+AC +    +  LL++
Sbjct:  494 QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 4 Score: 57.7658 bits (138), Expect = 6.403e-11
Identity = 25/88 (28.41%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    ++++ LL     V+A  R +  P+H A+R G++ IV LL+ H   ++A  + +   +H+A K G  ++  +LI++
Sbjct:  437 ETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIEN 524          

HSP 5 Score: 56.225 bits (134), Expect = 2.361e-10
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A   G  N+V+FLL    NV+A       P+H+  ++GH  IV LL++HK +  A   +   P+H+A K G+  ++  L
Sbjct:  704 LHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          

HSP 6 Score: 55.0694 bits (131), Expect = 6.696e-10
Identity = 28/94 (29.79%), Postives = 50/94 (53.19%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DVT +S    +H A   G+ N+   L+ +  +V  + +  I P+H A + G   +V LL++   +IEA  R  + P+H A + G   +V +L++
Sbjct:  199 DVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLE 292          

HSP 7 Score: 52.373 bits (124), Expect = 5.708e-9
Identity = 30/99 (30.30%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D++A  K  VT   +H AC   +  V   LL++  +  AT +    P+H A RK  + I   L+++     A  ++   P+HL+ + G  +I  LLI+H
Sbjct:  560 DVDAQGKNGVT--PLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEH 656          

HSP 8 Score: 50.8322 bits (120), Expect = 1.750e-8
Identity = 27/98 (27.55%), Postives = 50/98 (51.02%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +IEA  +  +T   +H A + GH  VV  LL++   + A  +  + P+H A +  H+    +L+ H+  ++ V    +  +H+A   G   + KLL+D
Sbjct:  263 NIEAKTRDGLT--PLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 9 Score: 50.8322 bits (120), Expect = 1.802e-8
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A   G +N+V +LL  + + +  T+RG+  P+H A R     I+ +L+ +   ++A  R    P+H+A + G  DIV LL+ H
Sbjct:  407 LHVAAFMGCMNIVIYLLQHDASPDVPTVRGE-TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 491          

HSP 10 Score: 50.8322 bits (120), Expect = 1.837e-8
Identity = 25/85 (29.41%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A K+  +     LLD + N + T +    P+H A+  G+  I  LLI    D+    +  + P+H+A K G  ++V LL++
Sbjct:  175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLE 259          

HSP 11 Score: 50.0618 bits (118), Expect = 3.232e-8
Identity = 24/85 (28.24%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      +  +H A   GH+++ +LL+D   D  A   +   P+H+ACK     +V+LL+ H
Sbjct:  308 LHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRH 392          

HSP 12 Score: 49.6766 bits (117), Expect = 4.329e-8
Identity = 22/83 (26.51%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A + G++++V  LL     V+AT +     +H A ++G  ++  +LI++   ++A  +    P+HL  K+G   + +LL+
Sbjct:  473 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 13 Score: 47.3654 bits (111), Expect = 3.516e-7
Identity = 22/86 (25.58%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K GH++VV  LL +   V++  +     +H A+  G  ++V+LL++H   +    ++   P+++A +     +V+LL+ +
Sbjct:   47 ALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSN 132          

HSP 14 Score: 45.4394 bits (106), Expect = 1.638e-6
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIK--PIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            A +QGH  VV  LL      E+  RGK++   +H A +K  +K   LL+D+  + +   +S   P+H+A  +G  +I  LLI
Sbjct:  150 AMQQGHDKVVAVLL------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI 225          

HSP 15 Score: 45.4394 bits (106), Expect = 1.654e-6
Identity = 19/82 (23.17%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A ++  +++   LL+      A  +    P+H ++++GH +I  LLI+HK  +    ++ + P+HL  +    ++ ++L
Sbjct:  605 LHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL 686          

HSP 16 Score: 45.0542 bits (105), Expect = 1.952e-6
Identity = 20/85 (23.53%), Postives = 41/85 (48.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++ ++NV + L     N++   +    P+H A+  G   +V  L+ +  +++A       P+H   + G   IV LL++H
Sbjct:  671 MHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEH 755          

HSP 17 Score: 44.669 bits (104), Expect = 2.419e-6
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   K GH+ V + LL +  +V+A  +  + P+H A    + ++  LL++      A  ++   P+H+A +    DI   L+++
Sbjct:  539 LHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEY 623          
BLAST of SMED30030283 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303121)

HSP 1 Score: 63.929 bits (154), Expect = 3.933e-13
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTE-RSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD+V V    ++H A   GH+ V K LLD+N +  A       P+H A +K  +K+VELL+ H   I A   S + P+H+A   G  +IV  L+ H
Sbjct:  330 VDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH 425          

HSP 2 Score: 61.6178 bits (148), Expect = 2.894e-12
Identity = 28/85 (32.94%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+V  LL++  N+EA  R  + P+H A R GH ++V++L++    I A  ++ + P+H+A +    D  ++L+ H
Sbjct:  242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH 326          

HSP 3 Score: 59.3066 bits (142), Expect = 2.173e-11
Identity = 27/96 (28.12%), Postives = 53/96 (55.21%), Query Frame = 1
Query:   22 KVDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +VD T +    ++H A K+G   V   L++    ++A  +    P+H   + GHIK+ +LL+  + D++A  ++ V P+H+AC +    +  LL++
Sbjct:  494 QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 4 Score: 57.7658 bits (138), Expect = 6.530e-11
Identity = 25/88 (28.41%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    ++++ LL     V+A  R +  P+H A+R G++ IV LL+ H   ++A  + +   +H+A K G  ++  +LI++
Sbjct:  437 ETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIEN 524          

HSP 5 Score: 56.225 bits (134), Expect = 2.249e-10
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A   G  N+V+FLL    NV+A       P+H+  ++GH  IV LL++HK +  A   +   P+H+A K G+  ++  L
Sbjct:  704 LHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          

HSP 6 Score: 55.0694 bits (131), Expect = 6.567e-10
Identity = 28/94 (29.79%), Postives = 50/94 (53.19%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DVT +S    +H A   G+ N+   L+ +  +V  + +  I P+H A + G   +V LL++   +IEA  R  + P+H A + G   +V +L++
Sbjct:  199 DVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLE 292          

HSP 7 Score: 52.373 bits (124), Expect = 5.653e-9
Identity = 30/99 (30.30%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D++A  K  VT   +H AC   +  V   LL++  +  AT +    P+H A RK  + I   L+++     A  ++   P+HL+ + G  +I  LLI+H
Sbjct:  560 DVDAQGKNGVT--PLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEH 656          

HSP 8 Score: 50.8322 bits (120), Expect = 1.733e-8
Identity = 27/98 (27.55%), Postives = 50/98 (51.02%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +IEA  +  +T   +H A + GH  VV  LL++   + A  +  + P+H A +  H+    +L+ H+  ++ V    +  +H+A   G   + KLL+D
Sbjct:  263 NIEAKTRDGLT--PLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 9 Score: 50.8322 bits (120), Expect = 1.837e-8
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A   G +N+V +LL  + + +  T+RG+  P+H A R     I+ +L+ +   ++A  R    P+H+A + G  DIV LL+ H
Sbjct:  407 LHVAAFMGCMNIVIYLLQHDASPDVPTVRGE-TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 491          

HSP 10 Score: 50.8322 bits (120), Expect = 1.873e-8
Identity = 25/85 (29.41%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A K+  +     LLD + N + T +    P+H A+  G+  I  LLI    D+    +  + P+H+A K G  ++V LL++
Sbjct:  175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLE 259          

HSP 11 Score: 50.0618 bits (118), Expect = 3.109e-8
Identity = 24/85 (28.24%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      +  +H A   GH+++ +LL+D   D  A   +   P+H+ACK     +V+LL+ H
Sbjct:  308 LHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRH 392          

HSP 12 Score: 49.6766 bits (117), Expect = 4.372e-8
Identity = 22/83 (26.51%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A + G++++V  LL     V+AT +     +H A ++G  ++  +LI++   ++A  +    P+HL  K+G   + +LL+
Sbjct:  473 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 13 Score: 47.3654 bits (111), Expect = 3.516e-7
Identity = 22/86 (25.58%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K GH++VV  LL +   V++  +     +H A+  G  ++V+LL++H   +    ++   P+++A +     +V+LL+ +
Sbjct:   47 ALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSN 132          

HSP 14 Score: 45.4394 bits (106), Expect = 1.623e-6
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIK--PIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            A +QGH  VV  LL      E+  RGK++   +H A +K  +K   LL+D+  + +   +S   P+H+A  +G  +I  LLI
Sbjct:  150 AMQQGHDKVVAVLL------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI 225          

HSP 15 Score: 45.4394 bits (106), Expect = 1.638e-6
Identity = 19/82 (23.17%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A ++  +++   LL+      A  +    P+H ++++GH +I  LLI+HK  +    ++ + P+HL  +    ++ ++L
Sbjct:  605 LHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL 686          

HSP 16 Score: 45.0542 bits (105), Expect = 1.934e-6
Identity = 20/85 (23.53%), Postives = 41/85 (48.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++ ++NV + L     N++   +    P+H A+  G   +V  L+ +  +++A       P+H   + G   IV LL++H
Sbjct:  671 MHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEH 755          

HSP 17 Score: 44.669 bits (104), Expect = 2.443e-6
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   K GH+ V + LL +  +V+A  +  + P+H A    + ++  LL++      A  ++   P+H+A +    DI   L+++
Sbjct:  539 LHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEY 623          
BLAST of SMED30030283 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303112)

HSP 1 Score: 63.929 bits (154), Expect = 3.935e-13
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTE-RSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD+V V    ++H A   GH+ V K LLD+N +  A       P+H A +K  +K+VELL+ H   I A   S + P+H+A   G  +IV  L+ H
Sbjct:  337 VDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH 432          

HSP 2 Score: 61.6178 bits (148), Expect = 3.011e-12
Identity = 28/85 (32.94%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+V  LL++  N+EA  R  + P+H A R GH ++V++L++    I A  ++ + P+H+A +    D  ++L+ H
Sbjct:  249 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH 333          

HSP 3 Score: 59.3066 bits (142), Expect = 2.217e-11
Identity = 27/96 (28.12%), Postives = 53/96 (55.21%), Query Frame = 1
Query:   22 KVDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +VD T +    ++H A K+G   V   L++    ++A  +    P+H   + GHIK+ +LL+  + D++A  ++ V P+H+AC +    +  LL++
Sbjct:  501 QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 596          

HSP 4 Score: 57.7658 bits (138), Expect = 6.408e-11
Identity = 25/88 (28.41%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    ++++ LL     V+A  R +  P+H A+R G++ IV LL+ H   ++A  + +   +H+A K G  ++  +LI++
Sbjct:  444 ETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIEN 531          

HSP 5 Score: 56.225 bits (134), Expect = 2.363e-10
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A   G  N+V+FLL    NV+A       P+H+  ++GH  IV LL++HK +  A   +   P+H+A K G+  ++  L
Sbjct:  711 LHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 792          

HSP 6 Score: 55.0694 bits (131), Expect = 6.700e-10
Identity = 28/94 (29.79%), Postives = 50/94 (53.19%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DVT +S    +H A   G+ N+   L+ +  +V  + +  I P+H A + G   +V LL++   +IEA  R  + P+H A + G   +V +L++
Sbjct:  206 DVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLE 299          

HSP 7 Score: 52.373 bits (124), Expect = 5.712e-9
Identity = 30/99 (30.30%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D++A  K  VT   +H AC   +  V   LL++  +  AT +    P+H A RK  + I   L+++     A  ++   P+HL+ + G  +I  LLI+H
Sbjct:  567 DVDAQGKNGVT--PLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEH 663          

HSP 8 Score: 50.8322 bits (120), Expect = 1.751e-8
Identity = 27/98 (27.55%), Postives = 50/98 (51.02%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +IEA  +  +T   +H A + GH  VV  LL++   + A  +  + P+H A +  H+    +L+ H+  ++ V    +  +H+A   G   + KLL+D
Sbjct:  270 NIEAKTRDGLT--PLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 365          

HSP 9 Score: 50.8322 bits (120), Expect = 1.803e-8
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A   G +N+V +LL  + + +  T+RG+  P+H A R     I+ +L+ +   ++A  R    P+H+A + G  DIV LL+ H
Sbjct:  414 LHVAAFMGCMNIVIYLLQHDASPDVPTVRGE-TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 498          

HSP 10 Score: 50.8322 bits (120), Expect = 1.838e-8
Identity = 25/85 (29.41%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A K+  +     LLD + N + T +    P+H A+  G+  I  LLI    D+    +  + P+H+A K G  ++V LL++
Sbjct:  182 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLE 266          

HSP 11 Score: 50.0618 bits (118), Expect = 3.234e-8
Identity = 24/85 (28.24%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      +  +H A   GH+++ +LL+D   D  A   +   P+H+ACK     +V+LL+ H
Sbjct:  315 LHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRH 399          

HSP 12 Score: 49.6766 bits (117), Expect = 4.332e-8
Identity = 22/83 (26.51%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A + G++++V  LL     V+AT +     +H A ++G  ++  +LI++   ++A  +    P+HL  K+G   + +LL+
Sbjct:  480 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL 562          

HSP 13 Score: 47.3654 bits (111), Expect = 3.518e-7
Identity = 22/86 (25.58%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K GH++VV  LL +   V++  +     +H A+  G  ++V+LL++H   +    ++   P+++A +     +V+LL+ +
Sbjct:   54 ALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSN 139          

HSP 14 Score: 45.4394 bits (106), Expect = 1.639e-6
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIK--PIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            A +QGH  VV  LL      E+  RGK++   +H A +K  +K   LL+D+  + +   +S   P+H+A  +G  +I  LLI
Sbjct:  157 AMQQGHDKVVAVLL------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI 232          

HSP 15 Score: 45.4394 bits (106), Expect = 1.656e-6
Identity = 19/82 (23.17%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A ++  +++   LL+      A  +    P+H ++++GH +I  LLI+HK  +    ++ + P+HL  +    ++ ++L
Sbjct:  612 LHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL 693          

HSP 16 Score: 45.0542 bits (105), Expect = 1.954e-6
Identity = 20/85 (23.53%), Postives = 41/85 (48.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++ ++NV + L     N++   +    P+H A+  G   +V  L+ +  +++A       P+H   + G   IV LL++H
Sbjct:  678 MHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEH 762          

HSP 17 Score: 44.669 bits (104), Expect = 2.421e-6
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   K GH+ V + LL +  +V+A  +  + P+H A    + ++  LL++      A  ++   P+H+A +    DI   L+++
Sbjct:  546 LHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEY 630          
BLAST of SMED30030283 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303123)

HSP 1 Score: 63.929 bits (154), Expect = 4.011e-13
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTE-RSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD+V V    ++H A   GH+ V K LLD+N +  A       P+H A +K  +K+VELL+ H   I A   S + P+H+A   G  +IV  L+ H
Sbjct:  330 VDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH 425          

HSP 2 Score: 61.6178 bits (148), Expect = 3.010e-12
Identity = 28/85 (32.94%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+V  LL++  N+EA  R  + P+H A R GH ++V++L++    I A  ++ + P+H+A +    D  ++L+ H
Sbjct:  242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH 326          

HSP 3 Score: 59.3066 bits (142), Expect = 2.216e-11
Identity = 27/96 (28.12%), Postives = 53/96 (55.21%), Query Frame = 1
Query:   22 KVDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +VD T +    ++H A K+G   V   L++    ++A  +    P+H   + GHIK+ +LL+  + D++A  ++ V P+H+AC +    +  LL++
Sbjct:  494 QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 4 Score: 57.7658 bits (138), Expect = 6.405e-11
Identity = 25/88 (28.41%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    ++++ LL     V+A  R +  P+H A+R G++ IV LL+ H   ++A  + +   +H+A K G  ++  +LI++
Sbjct:  437 ETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIEN 524          

HSP 5 Score: 56.225 bits (134), Expect = 2.362e-10
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A   G  N+V+FLL    NV+A       P+H+  ++GH  IV LL++HK +  A   +   P+H+A K G+  ++  L
Sbjct:  704 LHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          

HSP 6 Score: 55.0694 bits (131), Expect = 6.698e-10
Identity = 28/94 (29.79%), Postives = 50/94 (53.19%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DVT +S    +H A   G+ N+   L+ +  +V  + +  I P+H A + G   +V LL++   +IEA  R  + P+H A + G   +V +L++
Sbjct:  199 DVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLE 292          

HSP 7 Score: 52.373 bits (124), Expect = 5.709e-9
Identity = 30/99 (30.30%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D++A  K  VT   +H AC   +  V   LL++  +  AT +    P+H A RK  + I   L+++     A  ++   P+HL+ + G  +I  LLI+H
Sbjct:  560 DVDAQGKNGVT--PLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEH 656          

HSP 8 Score: 50.8322 bits (120), Expect = 1.750e-8
Identity = 27/98 (27.55%), Postives = 50/98 (51.02%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +IEA  +  +T   +H A + GH  VV  LL++   + A  +  + P+H A +  H+    +L+ H+  ++ V    +  +H+A   G   + KLL+D
Sbjct:  263 NIEAKTRDGLT--PLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 9 Score: 50.8322 bits (120), Expect = 1.838e-8
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A   G +N+V +LL  + + +  T+RG+  P+H A R     I+ +L+ +   ++A  R    P+H+A + G  DIV LL+ H
Sbjct:  407 LHVAAFMGCMNIVIYLLQHDASPDVPTVRGE-TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 491          

HSP 10 Score: 50.8322 bits (120), Expect = 1.856e-8
Identity = 25/85 (29.41%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A K+  +     LLD + N + T +    P+H A+  G+  I  LLI    D+    +  + P+H+A K G  ++V LL++
Sbjct:  175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLE 259          

HSP 11 Score: 50.0618 bits (118), Expect = 3.233e-8
Identity = 24/85 (28.24%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      +  +H A   GH+++ +LL+D   D  A   +   P+H+ACK     +V+LL+ H
Sbjct:  308 LHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRH 392          

HSP 12 Score: 49.6766 bits (117), Expect = 4.330e-8
Identity = 22/83 (26.51%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A + G++++V  LL     V+AT +     +H A ++G  ++  +LI++   ++A  +    P+HL  K+G   + +LL+
Sbjct:  473 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 13 Score: 47.3654 bits (111), Expect = 3.517e-7
Identity = 22/86 (25.58%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K GH++VV  LL +   V++  +     +H A+  G  ++V+LL++H   +    ++   P+++A +     +V+LL+ +
Sbjct:   47 ALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSN 132          

HSP 14 Score: 45.4394 bits (106), Expect = 1.639e-6
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIK--PIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            A +QGH  VV  LL      E+  RGK++   +H A +K  +K   LL+D+  + +   +S   P+H+A  +G  +I  LLI
Sbjct:  150 AMQQGHDKVVAVLL------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI 225          

HSP 15 Score: 45.4394 bits (106), Expect = 1.655e-6
Identity = 19/82 (23.17%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A ++  +++   LL+      A  +    P+H ++++GH +I  LLI+HK  +    ++ + P+HL  +    ++ ++L
Sbjct:  605 LHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL 686          

HSP 16 Score: 45.0542 bits (105), Expect = 1.972e-6
Identity = 20/85 (23.53%), Postives = 41/85 (48.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++ ++NV + L     N++   +    P+H A+  G   +V  L+ +  +++A       P+H   + G   IV LL++H
Sbjct:  671 MHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEH 755          

HSP 17 Score: 44.669 bits (104), Expect = 2.420e-6
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   K GH+ V + LL +  +V+A  +  + P+H A    + ++  LL++      A  ++   P+H+A +    DI   L+++
Sbjct:  539 LHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEY 623          
BLAST of SMED30030283 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303114)

HSP 1 Score: 63.929 bits (154), Expect = 4.252e-13
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:   16 VDKVDVTE-RSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD+V V    ++H A   GH+ V K LLD+N +  A       P+H A +K  +K+VELL+ H   I A   S + P+H+A   G  +IV  L+ H
Sbjct:  330 VDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQH 425          

HSP 2 Score: 61.6178 bits (148), Expect = 3.318e-12
Identity = 28/85 (32.94%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G  N+V  LL++  N+EA  R  + P+H A R GH ++V++L++    I A  ++ + P+H+A +    D  ++L+ H
Sbjct:  242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYH 326          

HSP 3 Score: 58.9214 bits (141), Expect = 2.372e-11
Identity = 27/96 (28.12%), Postives = 53/96 (55.21%), Query Frame = 1
Query:   22 KVDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +VD T +    ++H A K+G   V   L++    ++A  +    P+H   + GHIK+ +LL+  + D++A  ++ V P+H+AC +    +  LL++
Sbjct:  494 QVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLE 589          

HSP 4 Score: 57.7658 bits (138), Expect = 6.992e-11
Identity = 25/88 (28.41%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +    ++++ LL     V+A  R +  P+H A+R G++ IV LL+ H   ++A  + +   +H+A K G  ++  +LI++
Sbjct:  437 ETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIEN 524          

HSP 5 Score: 56.225 bits (134), Expect = 2.554e-10
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A   G  N+V+FLL    NV+A       P+H+  ++GH  IV LL++HK +  A   +   P+H+A K G+  ++  L
Sbjct:  704 LHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSL 785          

HSP 6 Score: 54.6842 bits (130), Expect = 7.312e-10
Identity = 28/94 (29.79%), Postives = 50/94 (53.19%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DVT +S    +H A   G+ N+   L+ +  +V  + +  I P+H A + G   +V LL++   +IEA  R  + P+H A + G   +V +L++
Sbjct:  199 DVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLE 292          

HSP 7 Score: 52.373 bits (124), Expect = 6.113e-9
Identity = 30/99 (30.30%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D++A  K  VT   +H AC   +  V   LL++  +  AT +    P+H A RK  + I   L+++     A  ++   P+HL+ + G  +I  LLI+H
Sbjct:  560 DVDAQGKNGVT--PLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEH 656          

HSP 8 Score: 50.8322 bits (120), Expect = 1.838e-8
Identity = 27/98 (27.55%), Postives = 50/98 (51.02%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +IEA  +  +T   +H A + GH  VV  LL++   + A  +  + P+H A +  H+    +L+ H+  ++ V    +  +H+A   G   + KLL+D
Sbjct:  263 NIEAKTRDGLT--PLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLD 358          

HSP 9 Score: 50.8322 bits (120), Expect = 1.967e-8
Identity = 25/85 (29.41%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A K+  +     LLD + N + T +    P+H A+  G+  I  LLI    D+    +  + P+H+A K G  ++V LL++
Sbjct:  175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLE 259          

HSP 10 Score: 50.8322 bits (120), Expect = 2.006e-8
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A   G +N+V +LL  + + +  T+RG+  P+H A R     I+ +L+ +   ++A  R    P+H+A + G  DIV LL+ H
Sbjct:  407 LHVAAFMGCMNIVIYLLQHDASPDVPTVRGE-TPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQH 491          

HSP 11 Score: 50.0618 bits (118), Expect = 3.362e-8
Identity = 24/85 (28.24%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  H++  + LL     V+      +  +H A   GH+++ +LL+D   D  A   +   P+H+ACK     +V+LL+ H
Sbjct:  308 LHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRH 392          

HSP 12 Score: 49.6766 bits (117), Expect = 4.682e-8
Identity = 22/83 (26.51%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A + G++++V  LL     V+AT +     +H A ++G  ++  +LI++   ++A  +    P+HL  K+G   + +LL+
Sbjct:  473 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLL 555          

HSP 13 Score: 46.9802 bits (110), Expect = 3.692e-7
Identity = 22/86 (25.58%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K GH++VV  LL +   V++  +     +H A+  G  ++V+LL++H   +    ++   P+++A +     +V+LL+ +
Sbjct:   47 ALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSN 132          

HSP 14 Score: 45.0542 bits (105), Expect = 1.688e-6
Identity = 19/82 (23.17%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H A ++  +++   LL+      A  +    P+H ++++GH +I  LLI+HK  +    ++ + P+HL  +    ++ ++L
Sbjct:  605 LHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEIL 686          

HSP 15 Score: 45.0542 bits (105), Expect = 1.721e-6
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIK--PIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            A +QGH  VV  LL      E+  RGK++   +H A +K  +K   LL+D+  + +   +S   P+H+A  +G  +I  LLI
Sbjct:  150 AMQQGHDKVVAVLL------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLI 225          

HSP 16 Score: 45.0542 bits (105), Expect = 2.071e-6
Identity = 20/85 (23.53%), Postives = 41/85 (48.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++ ++NV + L     N++   +    P+H A+  G   +V  L+ +  +++A       P+H   + G   IV LL++H
Sbjct:  671 MHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEH 755          

HSP 17 Score: 44.669 bits (104), Expect = 2.516e-6
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   K GH+ V + LL +  +V+A  +  + P+H A    + ++  LL++      A  ++   P+H+A +    DI   L+++
Sbjct:  539 LHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEY 623          
BLAST of SMED30030283 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 68.9366 bits (167), Expect = 8.953e-15
Identity = 30/90 (33.33%), Postives = 51/90 (56.67%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D  E  +H A + GH  V +FLL  N  V+A  +    P+H A R GH ++V+LL++HK + ++   +   P+H+A + G     ++L+D
Sbjct:  421 DYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 510          

HSP 2 Score: 60.077 bits (144), Expect = 1.551e-11
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  +VK LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  325 LHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLL 407          

HSP 3 Score: 59.3066 bits (142), Expect = 2.313e-11
Identity = 25/83 (30.12%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH    + LLD+N       +    P+H A + G + + ELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  493 LHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 575          

HSP 4 Score: 58.9214 bits (141), Expect = 3.447e-11
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  +  + +  R    P+H A + G  DIV LL+ H
Sbjct:  658 LHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKH 742          

HSP 5 Score: 58.151 bits (139), Expect = 7.156e-11
Identity = 33/102 (32.35%), Postives = 58/102 (56.86%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQ----NCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +    N N+ ++   +  P+H A R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  380 LEAVTESGLT--PLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVE-TPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEH 478          

HSP 6 Score: 56.6102 bits (135), Expect = 2.009e-10
Identity = 26/82 (31.71%), Postives = 46/82 (56.10%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  +V +  R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  691 LHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 772          

HSP 7 Score: 56.6102 bits (135), Expect = 2.418e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  625 LHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 709          

HSP 8 Score: 50.0618 bits (118), Expect = 4.221e-8
Identity = 25/85 (29.41%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++V++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  292 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 376          

HSP 9 Score: 49.2914 bits (116), Expect = 7.722e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL    ++E T +     +H A   G  K+V  LI++  ++ A  +    P+++A +    ++VK L++H
Sbjct:   61 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEH 145          

HSP 10 Score: 49.2914 bits (116), Expect = 8.029e-8
Identity = 24/84 (28.57%), Postives = 43/84 (51.19%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A KQ  L V   LL    N  +     I P+H A+++G   +V LLI  + ++   +++ + P+HL  + G   I  +L+
Sbjct:  591 ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLV 674          

HSP 11 Score: 48.521 bits (114), Expect = 1.385e-7
Identity = 23/83 (27.71%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R     +H A ++  +++   L+ +  +  +     + P+HLA + G PD+V LLI
Sbjct:  559 LHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLI 641          

HSP 12 Score: 46.2098 bits (108), Expect = 1.031e-6
Identity = 26/99 (26.26%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D+E   K   T  ++H A   G   VV  L++   NV A  +    P++ A ++ H+++V+ L++H  +          P+ +A + G  ++V LLI++
Sbjct:   82 DLETTTKKGNT--ALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINY 178          

HSP 13 Score: 44.669 bits (104), Expect = 4.031e-6
Identity = 25/92 (27.17%), Postives = 46/92 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEAT--------MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A    +L+V + LL++  NV  T        +   + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   +VK+L+D
Sbjct:  251 LHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLD 342          
BLAST of SMED30030283 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 68.9366 bits (167), Expect = 8.956e-15
Identity = 30/90 (33.33%), Postives = 51/90 (56.67%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D  E  +H A + GH  V +FLL  N  V+A  +    P+H A R GH ++V+LL++HK + ++   +   P+H+A + G     ++L+D
Sbjct:  433 DYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 522          

HSP 2 Score: 60.077 bits (144), Expect = 1.567e-11
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  +VK LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  337 LHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLL 419          

HSP 3 Score: 59.3066 bits (142), Expect = 2.359e-11
Identity = 25/83 (30.12%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH    + LLD+N       +    P+H A + G + + ELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  505 LHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 587          

HSP 4 Score: 58.9214 bits (141), Expect = 3.382e-11
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  +  + +  R    P+H A + G  DIV LL+ H
Sbjct:  670 LHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKH 754          

HSP 5 Score: 58.151 bits (139), Expect = 7.228e-11
Identity = 33/102 (32.35%), Postives = 58/102 (56.86%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQ----NCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +    N N+ ++   +  P+H A R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  392 LEAVTESGLT--PLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVE-TPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEH 490          

HSP 6 Score: 56.6102 bits (135), Expect = 2.010e-10
Identity = 26/82 (31.71%), Postives = 46/82 (56.10%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  +V +  R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  703 LHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 784          

HSP 7 Score: 56.6102 bits (135), Expect = 2.418e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  637 LHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 721          

HSP 8 Score: 50.0618 bits (118), Expect = 4.223e-8
Identity = 25/85 (29.41%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++V++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  304 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 388          

HSP 9 Score: 49.2914 bits (116), Expect = 7.877e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL    ++E T +     +H A   G  K+V  LI++  ++ A  +    P+++A +    ++VK L++H
Sbjct:   73 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEH 157          

HSP 10 Score: 49.2914 bits (116), Expect = 8.031e-8
Identity = 24/84 (28.57%), Postives = 43/84 (51.19%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A KQ  L V   LL    N  +     I P+H A+++G   +V LLI  + ++   +++ + P+HL  + G   I  +L+
Sbjct:  603 ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLV 686          

HSP 11 Score: 48.521 bits (114), Expect = 1.372e-7
Identity = 23/83 (27.71%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R     +H A ++  +++   L+ +  +  +     + P+HLA + G PD+V LLI
Sbjct:  571 LHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLI 653          

HSP 12 Score: 46.2098 bits (108), Expect = 1.041e-6
Identity = 26/99 (26.26%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D+E   K   T  ++H A   G   VV  L++   NV A  +    P++ A ++ H+++V+ L++H  +          P+ +A + G  ++V LLI++
Sbjct:   94 DLETTTKKGNT--ALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINY 190          

HSP 13 Score: 44.669 bits (104), Expect = 4.032e-6
Identity = 25/92 (27.17%), Postives = 46/92 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEAT--------MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A    +L+V + LL++  NV  T        +   + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   +VK+L+D
Sbjct:  263 LHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLD 354          
BLAST of SMED30030283 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 68.9366 bits (167), Expect = 1.143e-14
Identity = 30/90 (33.33%), Postives = 51/90 (56.67%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D  E  +H A + GH  V +FLL  N  V+A  +    P+H A R GH ++V+LL++HK + ++   +   P+H+A + G     ++L+D
Sbjct:  449 DYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 538          

HSP 2 Score: 65.855 bits (159), Expect = 1.408e-13
Identity = 26/85 (30.59%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH+++VE+L+D    ++A  ++ + PIH+A +    D V+ L+ +
Sbjct:  254 LHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQY 338          

HSP 3 Score: 61.6178 bits (148), Expect = 3.745e-12
Identity = 28/85 (32.94%), Postives = 52/85 (61.18%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +L+V + LL++  NV  T +  I P+H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   +V++L+D 
Sbjct:  221 LHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQ 305          

HSP 4 Score: 60.8474 bits (146), Expect = 8.083e-12
Identity = 26/83 (31.33%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH    + LLD+N       +    P+H A + G + +VELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  521 LHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 603          

HSP 5 Score: 59.6918 bits (143), Expect = 1.629e-11
Identity = 31/97 (31.96%), Postives = 55/97 (56.70%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ VV+ LLDQ   ++A  +  + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   +VK+L+D
Sbjct:  276 IDAKTKDELT--PLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLD 370          

HSP 6 Score: 59.6918 bits (143), Expect = 1.904e-11
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  +VK LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  353 LHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLL 435          

HSP 7 Score: 58.5362 bits (140), Expect = 4.191e-11
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  +  + +  R    P+H A + G  DIV LL+ H
Sbjct:  686 LHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKH 770          

HSP 8 Score: 57.7658 bits (138), Expect = 9.683e-11
Identity = 33/102 (32.35%), Postives = 58/102 (56.86%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQ----NCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +    N N+ ++   +  P+H A R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  408 LEAVTESGLT--PLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVE-TPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEH 506          

HSP 9 Score: 56.6102 bits (135), Expect = 2.466e-10
Identity = 26/82 (31.71%), Postives = 46/82 (56.10%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  +V +  R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  719 LHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 800          

HSP 10 Score: 56.225 bits (134), Expect = 2.997e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  653 LHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 737          

HSP 11 Score: 49.6766 bits (117), Expect = 5.233e-8
Identity = 25/85 (29.41%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++V++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  320 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 404          

HSP 12 Score: 49.2914 bits (116), Expect = 9.573e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL    ++E T +     +H A   G  K+V  LI++  ++ A  +    P+++A +    ++VK L++H
Sbjct:   60 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEH 144          

HSP 13 Score: 49.2914 bits (116), Expect = 9.667e-8
Identity = 24/84 (28.57%), Postives = 43/84 (51.19%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A KQ  L V   LL    N  +     I P+H A+++G   +V LLI  + ++   +++ + P+HL  + G   I  +L+
Sbjct:  619 ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLV 702          

HSP 14 Score: 48.521 bits (114), Expect = 1.604e-7
Identity = 23/83 (27.71%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R     +H A ++  +++   L+ +  +  +     + P+HLA + G PD+V LLI
Sbjct:  587 LHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLI 669          

HSP 15 Score: 46.2098 bits (108), Expect = 1.159e-6
Identity = 26/99 (26.26%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D+E   K   T  ++H A   G   VV  L++   NV A  +    P++ A ++ H+++V+ L++H  +          P+ +A + G  ++V LLI++
Sbjct:   81 DLETTTKKGNT--ALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINY 177          
BLAST of SMED30030283 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 68.5514 bits (166), Expect = 1.235e-14
Identity = 30/90 (33.33%), Postives = 51/90 (56.67%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D  E  +H A + GH  V +FLL  N  V+A  +    P+H A R GH ++V+LL++HK + ++   +   P+H+A + G     ++L+D
Sbjct:  468 DYVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 557          

HSP 2 Score: 65.4698 bits (158), Expect = 1.522e-13
Identity = 26/85 (30.59%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH+++VE+L+D    ++A  ++ + PIH+A +    D V+ L+ +
Sbjct:  273 LHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQY 357          

HSP 3 Score: 61.6178 bits (148), Expect = 4.089e-12
Identity = 28/85 (32.94%), Postives = 52/85 (61.18%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +L+V + LL++  NV  T +  I P+H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   +V++L+D 
Sbjct:  240 LHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQ 324          

HSP 4 Score: 60.8474 bits (146), Expect = 8.325e-12
Identity = 26/83 (31.33%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH    + LLD+N       +    P+H A + G + +VELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  540 LHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 622          

HSP 5 Score: 59.6918 bits (143), Expect = 1.745e-11
Identity = 31/97 (31.96%), Postives = 55/97 (56.70%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ VV+ LLDQ   ++A  +  + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   +VK+L+D
Sbjct:  295 IDAKTKDELT--PLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLD 389          

HSP 6 Score: 59.6918 bits (143), Expect = 1.961e-11
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  +VK LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  372 LHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLL 454          

HSP 7 Score: 58.5362 bits (140), Expect = 4.621e-11
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  +  + +  R    P+H A + G  DIV LL+ H
Sbjct:  705 LHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKH 789          

HSP 8 Score: 57.3806 bits (137), Expect = 1.057e-10
Identity = 33/102 (32.35%), Postives = 58/102 (56.86%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQ----NCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +    N N+ ++   +  P+H A R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  427 LEAVTESGLT--PLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVE-TPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEH 525          

HSP 9 Score: 56.225 bits (134), Expect = 2.616e-10
Identity = 26/82 (31.71%), Postives = 46/82 (56.10%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  +V +  R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  738 LHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 819          

HSP 10 Score: 56.225 bits (134), Expect = 3.272e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  672 LHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 756          

HSP 11 Score: 50.0618 bits (118), Expect = 5.133e-8
Identity = 25/85 (29.41%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++V++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  339 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 423          

HSP 12 Score: 48.9062 bits (115), Expect = 1.005e-7
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL    ++E T +     +H A   G  K+V  LI++  ++ A  +    P+++A +    ++VK L++H
Sbjct:   79 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEH 163          

HSP 13 Score: 48.9062 bits (115), Expect = 1.025e-7
Identity = 24/84 (28.57%), Postives = 43/84 (51.19%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A KQ  L V   LL    N  +     I P+H A+++G   +V LLI  + ++   +++ + P+HL  + G   I  +L+
Sbjct:  638 ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLV 721          

HSP 14 Score: 48.521 bits (114), Expect = 1.668e-7
Identity = 23/83 (27.71%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R     +H A ++  +++   L+ +  +  +     + P+HLA + G PD+V LLI
Sbjct:  606 LHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLI 688          

HSP 15 Score: 46.595 bits (109), Expect = 7.929e-7
Identity = 22/85 (25.88%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK G ++VV+ LL++  N  A  +  + P+H A    ++ +V+LL+       +  R+    +H+A K    ++   L+ +
Sbjct:  573 LHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQY 657          

HSP 16 Score: 45.8246 bits (107), Expect = 1.303e-6
Identity = 26/99 (26.26%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D+E   K   T  ++H A   G   VV  L++   NV A  +    P++ A ++ H+++V+ L++H  +          P+ +A + G  ++V LLI++
Sbjct:  100 DLETTTKKGNT--ALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINY 196          
BLAST of SMED30030283 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 67.0106 bits (162), Expect = 4.641e-14
Identity = 29/87 (33.33%), Postives = 50/87 (57.47%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V +FLL  N  V+A  +    P+H A R GH ++V+LL++HK + ++   +   P+H+A + G     ++L+D
Sbjct:  406 ETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 492          

HSP 2 Score: 62.003 bits (149), Expect = 2.440e-12
Identity = 32/98 (32.65%), Postives = 55/98 (56.12%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +  +  A+      P+H A R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  365 LEAVTESGLT--PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEH 460          

HSP 3 Score: 60.4622 bits (145), Expect = 1.083e-11
Identity = 29/97 (29.90%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++A  K D T   +H A + GH  +VK L++   N ++       P+H A R+GH +   +L+D       + +    P+H+ACK+G  D+ +LL++
Sbjct:  431 VDAKAKDDQT--PLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLE 525          

HSP 4 Score: 59.6918 bits (143), Expect = 1.678e-11
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  +VK LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  310 LHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLL 392          

HSP 5 Score: 59.3066 bits (142), Expect = 2.730e-11
Identity = 25/83 (30.12%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH    + LLD+N       +    P+H A + G + + ELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  475 LHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 557          

HSP 6 Score: 58.9214 bits (141), Expect = 3.802e-11
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  +  + +  R    P+H A + G  DIV LL+ H
Sbjct:  640 LHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKH 724          

HSP 7 Score: 56.6102 bits (135), Expect = 2.194e-10
Identity = 26/82 (31.71%), Postives = 46/82 (56.10%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  +V +  R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  673 LHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 754          

HSP 8 Score: 56.225 bits (134), Expect = 2.719e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  607 LHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 691          

HSP 9 Score: 53.9138 bits (128), Expect = 1.964e-9
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+  +  LL  + ++EA     + P+H A   GH+ IV+ L+       A +  +  P+H+A + G  ++ + L+ +
Sbjct:  343 LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQN 427          

HSP 10 Score: 50.0618 bits (118), Expect = 4.566e-8
Identity = 25/85 (29.41%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++V++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  277 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 361          

HSP 11 Score: 49.2914 bits (116), Expect = 8.684e-8
Identity = 24/84 (28.57%), Postives = 43/84 (51.19%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A KQ  L V   LL    N  +     I P+H A+++G   +V LLI  + ++   +++ + P+HL  + G   I  +L+
Sbjct:  573 ALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLV 656          

HSP 12 Score: 49.2914 bits (116), Expect = 8.855e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL    ++E T +     +H A   G  K+V  LI++  ++ A  +    P+++A +    ++VK L++H
Sbjct:   46 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEH 130          

HSP 13 Score: 48.521 bits (114), Expect = 1.455e-7
Identity = 23/83 (27.71%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R     +H A ++  +++   L+ +  +  +     + P+HLA + G PD+V LLI
Sbjct:  541 LHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLI 623          

HSP 14 Score: 46.2098 bits (108), Expect = 1.083e-6
Identity = 26/99 (26.26%), Postives = 50/99 (50.51%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D+E   K   T  ++H A   G   VV  L++   NV A  +    P++ A ++ H+++V+ L++H  +          P+ +A + G  ++V LLI++
Sbjct:   67 DLETTTKKGNT--ALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLEHGANQSLPTEDGFTPLAVALQQGHENVVALLINY 163          

HSP 15 Score: 44.2838 bits (103), Expect = 4.359e-6
Identity = 25/92 (27.17%), Postives = 46/92 (50.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEAT--------MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A    +L+V + LL++  NV  T        +   + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   +VK+L+D
Sbjct:  236 LHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLD 327          
BLAST of SMED30030283 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 70.4774 bits (171), Expect = 3.853e-15
Identity = 29/86 (33.72%), Postives = 57/86 (66.28%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  233 ALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQY 318          

HSP 2 Score: 70.4774 bits (171), Expect = 4.205e-15
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH ++V  LL +  NV    +  + P+H A+++GH+ I E+L+ H   + A  R    P+H+AC +G   +VK L+ H
Sbjct:  572 LHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQH 656          

HSP 3 Score: 68.1662 bits (165), Expect = 2.521e-14
Identity = 30/83 (36.14%), Postives = 50/83 (60.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GHLN+ + L+  + +V A  R    P+H A   G+IK+V+ L+ H+ ++ AV +    P+H A + G  DIV LL+
Sbjct:  605 LHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 687          

HSP 4 Score: 62.003 bits (149), Expect = 4.475e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  NV  T +  I  +H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I ++L+DH
Sbjct:  201 LHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDH 285          

HSP 5 Score: 55.0694 bits (131), Expect = 9.861e-10
Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            +H AC  G++ VVKFLL    NV A  +    P+H+A ++GH  IV LL+ +         + + P+ +A + G+    D++ L+ D
Sbjct:  638 LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVLSLVTD 724          

HSP 6 Score: 54.6842 bits (130), Expect = 1.414e-9
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  +V+ LL+   + +        P+H  TR GH +  ELL+D++    ++ +    P+H+A K+G   I +LL++
Sbjct:  407 LHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE 490          

HSP 7 Score: 51.9878 bits (123), Expect = 1.355e-8
Identity = 23/84 (27.38%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH    + LLD      +  +    P+H A + G I+I ELL++ +    +  ++ + P+H+A  H   +IV+LL++
Sbjct:  440 LHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 523          

HSP 8 Score: 51.9878 bits (123), Expect = 1.538e-8
Identity = 27/97 (27.84%), Postives = 48/97 (49.48%), Query Frame = 1
Query:   13 AVDKV-DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++D V +  E  +H A +  H +V  FL+     V A  +    P+H A R GH ++V LL+++    +    +   P+HL  + G     +LL+D+
Sbjct:  362 SIDAVTEKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDN 458          

HSP 9 Score: 51.6026 bits (122), Expect = 1.728e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  ++  + LL       A     + P+H A ++GH  +V LL+  + ++   ++S + P+HLA + G  +I ++L+ H
Sbjct:  539 LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSH 623          

HSP 10 Score: 51.6026 bits (122), Expect = 1.780e-8
Identity = 24/83 (28.92%), Postives = 47/83 (56.63%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLA 255
            I+A  K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A++  H+  V LL+ ++  I+ +    + P+H+A
Sbjct:  256 IDACTKDELT--PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVA 336          

HSP 11 Score: 50.8322 bits (120), Expect = 3.659e-8
Identity = 25/83 (30.12%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    HLN+V+ LL++  +   T R    P+H A ++  +     L+       A     V P+HLA + G  D+V LL+
Sbjct:  506 LHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLL 588          

HSP 12 Score: 44.2838 bits (103), Expect = 6.329e-6
Identity = 21/83 (25.30%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A K G + + + LL++  +  +  +  + P+H A    H+ IV LL++         R+   P+H+A K    D  + L+
Sbjct:  473 LHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALL 555          
BLAST of SMED30030283 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 70.4774 bits (171), Expect = 4.126e-15
Identity = 29/86 (33.72%), Postives = 57/86 (66.28%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  275 ALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQY 360          

HSP 2 Score: 70.4774 bits (171), Expect = 4.548e-15
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH ++V  LL +  NV    +  + P+H A+++GH+ I E+L+ H   + A  R    P+H+AC +G   +VK L+ H
Sbjct:  614 LHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQH 698          

HSP 3 Score: 68.1662 bits (165), Expect = 2.834e-14
Identity = 30/83 (36.14%), Postives = 50/83 (60.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GHLN+ + L+  + +V A  R    P+H A   G+IK+V+ L+ H+ ++ AV +    P+H A + G  DIV LL+
Sbjct:  647 LHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 729          

HSP 4 Score: 61.6178 bits (148), Expect = 5.081e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  NV  T +  I  +H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I ++L+DH
Sbjct:  243 LHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDH 327          

HSP 5 Score: 55.0694 bits (131), Expect = 1.087e-9
Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            +H AC  G++ VVKFLL    NV A  +    P+H+A ++GH  IV LL+ +         + + P+ +A + G+    D++ L+ D
Sbjct:  680 LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVLSLVTD 766          

HSP 6 Score: 54.6842 bits (130), Expect = 1.544e-9
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  +V+ LL+   + +        P+H  TR GH +  ELL+D++    ++ +    P+H+A K+G   I +LL++
Sbjct:  449 LHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE 532          

HSP 7 Score: 51.9878 bits (123), Expect = 1.451e-8
Identity = 23/84 (27.38%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH    + LLD      +  +    P+H A + G I+I ELL++ +    +  ++ + P+H+A  H   +IV+LL++
Sbjct:  482 LHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 565          

HSP 8 Score: 51.6026 bits (122), Expect = 1.663e-8
Identity = 27/97 (27.84%), Postives = 48/97 (49.48%), Query Frame = 1
Query:   13 AVDKV-DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++D V +  E  +H A +  H +V  FL+     V A  +    P+H A R GH ++V LL+++    +    +   P+HL  + G     +LL+D+
Sbjct:  404 SIDAVTEKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDN 500          

HSP 9 Score: 51.6026 bits (122), Expect = 1.887e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  ++  + LL       A     + P+H A ++GH  +V LL+  + ++   ++S + P+HLA + G  +I ++L+ H
Sbjct:  581 LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSH 665          

HSP 10 Score: 51.6026 bits (122), Expect = 1.906e-8
Identity = 24/83 (28.92%), Postives = 47/83 (56.63%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLA 255
            I+A  K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A++  H+  V LL+ ++  I+ +    + P+H+A
Sbjct:  298 IDACTKDELT--PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVA 378          

HSP 11 Score: 50.447 bits (119), Expect = 4.115e-8
Identity = 25/83 (30.12%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    HLN+V+ LL++  +   T R    P+H A ++  +     L+       A     V P+HLA + G  D+V LL+
Sbjct:  548 LHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLL 630          

HSP 12 Score: 44.2838 bits (103), Expect = 6.779e-6
Identity = 21/83 (25.30%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A K G + + + LL++  +  +  +  + P+H A    H+ IV LL++         R+   P+H+A K    D  + L+
Sbjct:  515 LHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALL 597          
BLAST of SMED30030283 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 70.4774 bits (171), Expect = 4.212e-15
Identity = 29/86 (33.72%), Postives = 57/86 (66.28%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  238 ALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQY 323          

HSP 2 Score: 70.4774 bits (171), Expect = 4.553e-15
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH ++V  LL +  NV    +  + P+H A+++GH+ I E+L+ H   + A  R    P+H+AC +G   +VK L+ H
Sbjct:  635 LHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQH 719          

HSP 3 Score: 68.1662 bits (165), Expect = 2.894e-14
Identity = 30/83 (36.14%), Postives = 50/83 (60.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GHLN+ + L+  + +V A  R    P+H A   G+IK+V+ L+ H+ ++ AV +    P+H A + G  DIV LL+
Sbjct:  668 LHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 750          

HSP 4 Score: 61.6178 bits (148), Expect = 5.088e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  NV  T +  I  +H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I ++L+DH
Sbjct:  206 LHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDH 290          

HSP 5 Score: 59.3066 bits (142), Expect = 4.049e-11
Identity = 29/97 (29.90%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A++  H+  V LL+ ++  I+ +    + P+H+A   G   + KLL+D
Sbjct:  261 IDACTKDELT--PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD 355          

HSP 6 Score: 58.5362 bits (140), Expect = 6.215e-11
Identity = 31/83 (37.35%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     A       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  338 LHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 420          

HSP 7 Score: 55.0694 bits (131), Expect = 1.121e-9
Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            +H AC  G++ VVKFLL    NV A  +    P+H+A ++GH  IV LL+ +         + + P+ +A + G+    D++ L+ D
Sbjct:  701 LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVLSLVTD 787          

HSP 8 Score: 54.6842 bits (130), Expect = 1.561e-9
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  +V+ LL+   + +        P+H  TR GH +  ELL+D++    ++ +    P+H+A K+G   I +LL++
Sbjct:  470 LHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE 553          

HSP 9 Score: 54.299 bits (129), Expect = 2.217e-9
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+            +  P+H+A +    D+   LI +
Sbjct:  371 LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQN 455          

HSP 10 Score: 51.9878 bits (123), Expect = 1.467e-8
Identity = 23/84 (27.38%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH    + LLD      +  +    P+H A + G I+I ELL++ +    +  ++ + P+H+A  H   +IV+LL++
Sbjct:  503 LHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 586          

HSP 11 Score: 51.6026 bits (122), Expect = 1.800e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  ++  + LL       A     + P+H A ++GH  +V LL+  + ++   ++S + P+HLA + G  +I ++L+ H
Sbjct:  602 LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSH 686          

HSP 12 Score: 51.6026 bits (122), Expect = 2.063e-8
Identity = 25/88 (28.41%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +  H +V  FL+     V A  +    P+H A R GH ++V LL+++    +    +   P+HL  + G     +LL+D+
Sbjct:  434 ETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDN 521          

HSP 13 Score: 50.447 bits (119), Expect = 4.040e-8
Identity = 25/83 (30.12%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    HLN+V+ LL++  +   T R    P+H A ++  +     L+       A     V P+HLA + G  D+V LL+
Sbjct:  569 LHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLL 651          

HSP 14 Score: 49.6766 bits (117), Expect = 9.804e-8
Identity = 24/83 (28.92%), Postives = 43/83 (51.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IH A +  HL+ V+ LL    +++      + P+H A   GH ++ +LL+D      A   +   P+H+ACK     +++LL+
Sbjct:  305 IHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLL 387          

HSP 15 Score: 44.2838 bits (103), Expect = 6.656e-6
Identity = 21/83 (25.30%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A K G + + + LL++  +  +  +  + P+H A    H+ IV LL++         R+   P+H+A K    D  + L+
Sbjct:  536 LHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALL 618          
BLAST of SMED30030283 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 70.4774 bits (171), Expect = 4.590e-15
Identity = 29/86 (33.72%), Postives = 57/86 (66.28%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  228 ALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQY 313          

HSP 2 Score: 70.0922 bits (170), Expect = 5.010e-15
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH ++V  LL +  NV    +  + P+H A+++GH+ I E+L+ H   + A  R    P+H+AC +G   +VK L+ H
Sbjct:  599 LHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQH 683          

HSP 3 Score: 68.1662 bits (165), Expect = 3.123e-14
Identity = 30/83 (36.14%), Postives = 50/83 (60.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GHLN+ + L+  + +V A  R    P+H A   G+IK+V+ L+ H+ ++ AV +    P+H A + G  DIV LL+
Sbjct:  632 LHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 714          

HSP 4 Score: 61.6178 bits (148), Expect = 5.438e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  NV  T +  I  +H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I ++L+DH
Sbjct:  196 LHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDH 280          

HSP 5 Score: 59.3066 bits (142), Expect = 3.703e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A       P+H A R  H  + + LI +K  + A  +    P+H A + G  ++V+LL+++
Sbjct:  368 LHIACKKSHIRVMELLLKSGASIDAVTEKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLEN 452          

HSP 6 Score: 58.9214 bits (141), Expect = 4.412e-11
Identity = 29/97 (29.90%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A++  H+  V LL+ ++  I+ +    + P+H+A   G   + KLL+D
Sbjct:  251 IDACTKDELT--PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD 345          

HSP 7 Score: 55.0694 bits (131), Expect = 1.210e-9
Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            +H AC  G++ VVKFLL    NV A  +    P+H+A ++GH  IV LL+ +         + + P+ +A + G+    D++ L+ D
Sbjct:  665 LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVLSLVTD 751          

HSP 8 Score: 54.6842 bits (130), Expect = 1.685e-9
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  +V+ LL+   + +        P+H  TR GH +  ELL+D++    ++ +    P+H+A K+G   I +LL++
Sbjct:  434 LHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE 517          

HSP 9 Score: 53.5286 bits (127), Expect = 4.209e-9
Identity = 30/93 (32.26%), Postives = 45/93 (48.39%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEA--------TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A   GH  V K LLD+     A        T  G   P+H A +K HI+++ELL+     I+AV   +  P+H+A +    D+   LI +
Sbjct:  328 LHVAAHCGHHRVAKLLLDKGAKSNARALIIILFTTNG-FTPLHIACKKSHIRVMELLLKSGASIDAVTEKVETPLHMAARAAHTDVADFLIQN 419          

HSP 10 Score: 51.6026 bits (122), Expect = 1.583e-8
Identity = 23/84 (27.38%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH    + LLD      +  +    P+H A + G I+I ELL++ +    +  ++ + P+H+A  H   +IV+LL++
Sbjct:  467 LHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 550          

HSP 11 Score: 51.6026 bits (122), Expect = 1.832e-8
Identity = 27/97 (27.84%), Postives = 48/97 (49.48%), Query Frame = 1
Query:   13 AVDKV-DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++D V +  E  +H A +  H +V  FL+     V A  +    P+H A R GH ++V LL+++    +    +   P+HL  + G     +LL+D+
Sbjct:  389 SIDAVTEKVETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDN 485          

HSP 12 Score: 51.6026 bits (122), Expect = 1.943e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  ++  + LL       A     + P+H A ++GH  +V LL+  + ++   ++S + P+HLA + G  +I ++L+ H
Sbjct:  566 LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSH 650          

HSP 13 Score: 50.447 bits (119), Expect = 4.318e-8
Identity = 25/83 (30.12%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    HLN+V+ LL++  +   T R    P+H A ++  +     L+       A     V P+HLA + G  D+V LL+
Sbjct:  533 LHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLL 615          

HSP 14 Score: 44.2838 bits (103), Expect = 7.045e-6
Identity = 21/83 (25.30%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A K G + + + LL++  +  +  +  + P+H A    H+ IV LL++         R+   P+H+A K    D  + L+
Sbjct:  500 LHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALL 582          
BLAST of SMED30030283 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 70.4774 bits (171), Expect = 4.597e-15
Identity = 29/86 (33.72%), Postives = 57/86 (66.28%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  200 ALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQY 285          

HSP 2 Score: 70.0922 bits (170), Expect = 5.067e-15
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH ++V  LL +  NV    +  + P+H A+++GH+ I E+L+ H   + A  R    P+H+AC +G   +VK L+ H
Sbjct:  597 LHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQH 681          

HSP 3 Score: 68.1662 bits (165), Expect = 3.158e-14
Identity = 30/83 (36.14%), Postives = 50/83 (60.24%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GHLN+ + L+  + +V A  R    P+H A   G+IK+V+ L+ H+ ++ AV +    P+H A + G  DIV LL+
Sbjct:  630 LHLASQEGHLNIAEVLVSHDASVAAETRMGFAPLHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLL 712          

HSP 4 Score: 61.6178 bits (148), Expect = 5.393e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  NV  T +  I  +H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I ++L+DH
Sbjct:  168 LHIAAHYENLNVAQLLLNRGANVNFTPQNGISALHIASRRGNVIMVRLLLDRGAQIDACTKDELTPLHCAVRNGHVRISEILLDH 252          

HSP 5 Score: 58.9214 bits (141), Expect = 4.462e-11
Identity = 29/97 (29.90%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A++  H+  V LL+ ++  I+ +    + P+H+A   G   + KLL+D
Sbjct:  223 IDACTKDELT--PLHCAVRNGHVRISEILLDHGAPIQAKTKNGLSPIHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLD 317          

HSP 6 Score: 58.5362 bits (140), Expect = 6.916e-11
Identity = 31/83 (37.35%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     A       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  300 LHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLL 382          

HSP 7 Score: 55.0694 bits (131), Expect = 1.224e-9
Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            +H AC  G++ VVKFLL    NV A  +    P+H+A ++GH  IV LL+ +         + + P+ +A + G+    D++ L+ D
Sbjct:  663 LHVACHYGNIKVVKFLLQHQANVNAVTKLGFTPLHQAAQQGHTDIVTLLLKNGACPNQASMNGITPLAIAKRLGYISVTDVLSLVTD 749          

HSP 8 Score: 54.6842 bits (130), Expect = 1.704e-9
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  +V+ LL+   + +        P+H  TR GH +  ELL+D++    ++ +    P+H+A K+G   I +LL++
Sbjct:  432 LHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLE 515          

HSP 9 Score: 53.9138 bits (128), Expect = 2.443e-9
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+            +  P+H+A +    D+   LI +
Sbjct:  333 LHIACKKSHIRVMELLLKSGASIDAVTESGLTPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQN 417          

HSP 10 Score: 51.6026 bits (122), Expect = 1.586e-8
Identity = 23/84 (27.38%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH    + LLD      +  +    P+H A + G I+I ELL++ +    +  ++ + P+H+A  H   +IV+LL++
Sbjct:  465 LHLTTRGGHTQTAELLLDNQAAHTSMTKKGFTPLHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLN 548          

HSP 11 Score: 51.6026 bits (122), Expect = 1.965e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  ++  + LL       A     + P+H A ++GH  +V LL+  + ++   ++S + P+HLA + G  +I ++L+ H
Sbjct:  564 LHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLLSKQANVHLGNKSSLTPLHLASQEGHLNIAEVLVSH 648          

HSP 12 Score: 51.2174 bits (121), Expect = 2.208e-8
Identity = 25/88 (28.41%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E  +H A +  H +V  FL+     V A  +    P+H A R GH ++V LL+++    +    +   P+HL  + G     +LL+D+
Sbjct:  396 ETPLHMAARAAHTDVADFLIQNKAKVNARAKDDQTPLHCAARSGHYEMVRLLLENGAHPDPPTTAGHTPLHLTTRGGHTQTAELLLDN 483          

HSP 13 Score: 50.447 bits (119), Expect = 4.325e-8
Identity = 25/83 (30.12%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    HLN+V+ LL++  +   T R    P+H A ++  +     L+       A     V P+HLA + G  D+V LL+
Sbjct:  531 LHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALLQFGATCNAQSLQGVTPLHLAAQEGHGDMVTLLL 613          

HSP 14 Score: 49.2914 bits (116), Expect = 1.080e-7
Identity = 24/83 (28.92%), Postives = 43/83 (51.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            IH A +  HL+ V+ LL    +++      + P+H A   GH ++ +LL+D      A   +   P+H+ACK     +++LL+
Sbjct:  267 IHMASQGDHLDCVRLLLQYQAHIDDITLDHLTPLHVAAHCGHHRVAKLLLDKGAKSNARALNGFTPLHIACKKSHIRVMELLL 349          

HSP 15 Score: 44.2838 bits (103), Expect = 7.194e-6
Identity = 21/83 (25.30%), Postives = 40/83 (48.19%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A K G + + + LL++  +  +  +  + P+H A    H+ IV LL++         R+   P+H+A K    D  + L+
Sbjct:  498 LHVAAKYGKIQIAELLLEREAHPNSAGKTGLTPLHVAVYHNHLNIVRLLLNKGASPHCTARNGYTPLHMAVKQRSVDTARALL 580          
BLAST of SMED30030283 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 69.707 bits (169), Expect = 6.373e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  247 LHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 331          

HSP 2 Score: 63.1586 bits (152), Expect = 1.109e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  676 LHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 758          

HSP 3 Score: 62.003 bits (149), Expect = 3.466e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  214 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDH 298          

HSP 4 Score: 60.4622 bits (145), Expect = 1.083e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H  +++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  643 LHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH 727          

HSP 5 Score: 56.6102 bits (135), Expect = 2.111e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  269 IETRTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 363          

HSP 6 Score: 56.6102 bits (135), Expect = 2.746e-10
Identity = 25/83 (30.12%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++    LL++  +     +    P+H A + G +++ ELL++H     A  ++ + P+H+A  H   DIVKLL+
Sbjct:  511 LHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 593          

HSP 7 Score: 55.0694 bits (131), Expect = 7.939e-10
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  346 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 428          

HSP 8 Score: 54.299 bits (129), Expect = 1.817e-9
Identity = 23/83 (27.71%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+
Sbjct:  379 LHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 461          

HSP 9 Score: 51.6026 bits (122), Expect = 1.365e-8
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V  +   P+ +A + G+    D++K++ D
Sbjct:  698 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 795          

HSP 10 Score: 49.6766 bits (117), Expect = 7.653e-8
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  610 LHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKH 694          

HSP 11 Score: 49.2914 bits (116), Expect = 9.120e-8
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A + GH  +VK LL+   +          P+H A R+GH+     L++ +     + +    P+H+A K+G   + +LL++H
Sbjct:  478 LHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEH 562          

HSP 12 Score: 44.2838 bits (103), Expect = 4.806e-6
Identity = 25/87 (28.74%), Postives = 40/87 (45.98%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL       A  +    P+H A R GH  +V+LL+++         +   P+H A + G  D    L++
Sbjct:  442 ETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLE 528          

HSP 13 Score: 43.8986 bits (102), Expect = 7.378e-6
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K    A  +    P+H A + G   +VKLL+++
Sbjct:  401 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLEN 496          
BLAST of SMED30030283 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 69.707 bits (169), Expect = 6.375e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  239 LHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 323          

HSP 2 Score: 63.1586 bits (152), Expect = 1.099e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  668 LHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 750          

HSP 3 Score: 62.003 bits (149), Expect = 3.433e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  206 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDH 290          

HSP 4 Score: 60.4622 bits (145), Expect = 1.083e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H  +++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  635 LHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH 719          

HSP 5 Score: 56.6102 bits (135), Expect = 2.091e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  261 IETRTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 355          

HSP 6 Score: 56.6102 bits (135), Expect = 2.747e-10
Identity = 25/83 (30.12%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++    LL++  +     +    P+H A + G +++ ELL++H     A  ++ + P+H+A  H   DIVKLL+
Sbjct:  503 LHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 585          

HSP 7 Score: 55.0694 bits (131), Expect = 7.941e-10
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  338 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 420          

HSP 8 Score: 54.299 bits (129), Expect = 1.800e-9
Identity = 23/83 (27.71%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+
Sbjct:  371 LHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 453          

HSP 9 Score: 51.6026 bits (122), Expect = 1.352e-8
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V  +   P+ +A + G+    D++K++ D
Sbjct:  690 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 787          

HSP 10 Score: 49.6766 bits (117), Expect = 7.655e-8
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  602 LHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKH 686          

HSP 11 Score: 49.2914 bits (116), Expect = 9.122e-8
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A + GH  +VK LL+   +          P+H A R+GH+     L++ +     + +    P+H+A K+G   + +LL++H
Sbjct:  470 LHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEH 554          

HSP 12 Score: 44.2838 bits (103), Expect = 4.715e-6
Identity = 25/87 (28.74%), Postives = 40/87 (45.98%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL       A  +    P+H A R GH  +V+LL+++         +   P+H A + G  D    L++
Sbjct:  434 ETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLE 520          

HSP 13 Score: 43.8986 bits (102), Expect = 7.237e-6
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K    A  +    P+H A + G   +VKLL+++
Sbjct:  393 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLEN 488          

HSP 14 Score: 43.5134 bits (101), Expect = 9.981e-6
Identity = 21/85 (24.71%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL +   +E T +     +H A   G  ++V  L+++  ++ A  +    P+++A +    ++VK L+++
Sbjct:   45 LHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLEN 129          
BLAST of SMED30030283 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 69.707 bits (169), Expect = 6.377e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  239 LHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 323          

HSP 2 Score: 63.1586 bits (152), Expect = 1.110e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  668 LHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 750          

HSP 3 Score: 62.003 bits (149), Expect = 3.467e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  206 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDH 290          

HSP 4 Score: 60.4622 bits (145), Expect = 1.083e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H  +++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  635 LHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH 719          

HSP 5 Score: 56.6102 bits (135), Expect = 2.112e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  261 IETRTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 355          

HSP 6 Score: 56.6102 bits (135), Expect = 2.748e-10
Identity = 25/83 (30.12%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++    LL++  +     +    P+H A + G +++ ELL++H     A  ++ + P+H+A  H   DIVKLL+
Sbjct:  503 LHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 585          

HSP 7 Score: 55.0694 bits (131), Expect = 7.944e-10
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  338 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 420          

HSP 8 Score: 54.299 bits (129), Expect = 1.818e-9
Identity = 23/83 (27.71%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+
Sbjct:  371 LHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 453          

HSP 9 Score: 51.6026 bits (122), Expect = 1.365e-8
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V  +   P+ +A + G+    D++K++ D
Sbjct:  690 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 787          

HSP 10 Score: 49.6766 bits (117), Expect = 7.657e-8
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  602 LHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKH 686          

HSP 11 Score: 49.2914 bits (116), Expect = 9.125e-8
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A + GH  +VK LL+   +          P+H A R+GH+     L++ +     + +    P+H+A K+G   + +LL++H
Sbjct:  470 LHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEH 554          

HSP 12 Score: 44.2838 bits (103), Expect = 4.762e-6
Identity = 25/87 (28.74%), Postives = 40/87 (45.98%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL       A  +    P+H A R GH  +V+LL+++         +   P+H A + G  D    L++
Sbjct:  434 ETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLE 520          

HSP 13 Score: 43.8986 bits (102), Expect = 7.382e-6
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K    A  +    P+H A + G   +VKLL+++
Sbjct:  393 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLEN 488          
BLAST of SMED30030283 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 69.707 bits (169), Expect = 6.436e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  247 LHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 331          

HSP 2 Score: 63.1586 bits (152), Expect = 1.109e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  676 LHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 758          

HSP 3 Score: 62.003 bits (149), Expect = 3.466e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  214 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDH 298          

HSP 4 Score: 60.4622 bits (145), Expect = 1.083e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H  +++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  643 LHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH 727          

HSP 5 Score: 56.6102 bits (135), Expect = 2.111e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  269 IETRTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 363          

HSP 6 Score: 56.6102 bits (135), Expect = 2.746e-10
Identity = 25/83 (30.12%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++    LL++  +     +    P+H A + G +++ ELL++H     A  ++ + P+H+A  H   DIVKLL+
Sbjct:  511 LHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 593          

HSP 7 Score: 55.0694 bits (131), Expect = 8.017e-10
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  346 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 428          

HSP 8 Score: 54.299 bits (129), Expect = 1.817e-9
Identity = 23/83 (27.71%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+
Sbjct:  379 LHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 461          

HSP 9 Score: 51.6026 bits (122), Expect = 1.352e-8
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V  +   P+ +A + G+    D++K++ D
Sbjct:  698 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 795          

HSP 10 Score: 49.2914 bits (116), Expect = 7.729e-8
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  610 LHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKH 694          

HSP 11 Score: 49.2914 bits (116), Expect = 9.121e-8
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A + GH  +VK LL+   +          P+H A R+GH+     L++ +     + +    P+H+A K+G   + +LL++H
Sbjct:  478 LHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEH 562          

HSP 12 Score: 44.2838 bits (103), Expect = 4.901e-6
Identity = 25/87 (28.74%), Postives = 40/87 (45.98%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL       A  +    P+H A R GH  +V+LL+++         +   P+H A + G  D    L++
Sbjct:  442 ETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLE 528          

HSP 13 Score: 43.8986 bits (102), Expect = 7.451e-6
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K    A  +    P+H A + G   +VKLL+++
Sbjct:  401 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLEN 496          
BLAST of SMED30030283 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 69.707 bits (169), Expect = 6.438e-15
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  247 LHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 331          

HSP 2 Score: 63.1586 bits (152), Expect = 1.110e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  676 LHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 758          

HSP 3 Score: 62.003 bits (149), Expect = 3.433e-12
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  214 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDH 298          

HSP 4 Score: 60.4622 bits (145), Expect = 1.073e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H  +++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  643 LHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH 727          

HSP 5 Score: 56.6102 bits (135), Expect = 2.112e-10
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  269 IETRTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 363          

HSP 6 Score: 56.6102 bits (135), Expect = 2.774e-10
Identity = 25/83 (30.12%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++    LL++  +     +    P+H A + G +++ ELL++H     A  ++ + P+H+A  H   DIVKLL+
Sbjct:  511 LHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 593          

HSP 7 Score: 55.0694 bits (131), Expect = 8.099e-10
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  346 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 428          

HSP 8 Score: 54.299 bits (129), Expect = 1.818e-9
Identity = 23/83 (27.71%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+
Sbjct:  379 LHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 461          

HSP 9 Score: 51.6026 bits (122), Expect = 1.339e-8
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V  +   P+ +A + G+    D++K++ D
Sbjct:  698 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 795          

HSP 10 Score: 49.2914 bits (116), Expect = 7.807e-8
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  610 LHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKH 694          

HSP 11 Score: 49.2914 bits (116), Expect = 9.035e-8
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A + GH  +VK LL+   +          P+H A R+GH+     L++ +     + +    P+H+A K+G   + +LL++H
Sbjct:  478 LHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEH 562          

HSP 12 Score: 44.2838 bits (103), Expect = 4.716e-6
Identity = 25/87 (28.74%), Postives = 40/87 (45.98%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL       A  +    P+H A R GH  +V+LL+++         +   P+H A + G  D    L++
Sbjct:  442 ETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLE 528          

HSP 13 Score: 43.8986 bits (102), Expect = 7.381e-6
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K    A  +    P+H A + G   +VKLL+++
Sbjct:  401 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLEN 496          
BLAST of SMED30030283 vs. UniProt/SwissProt
Match: sp|P16157|ANK1_HUMAN (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 70.0922 bits (170), Expect = 3.497e-14
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  243 LHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 327          

HSP 2 Score: 62.7734 bits (151), Expect = 1.203e-11
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  672 LHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 3 Score: 62.003 bits (149), Expect = 2.035e-11
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  210 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDH 294          

HSP 4 Score: 58.9214 bits (141), Expect = 2.462e-10
Identity = 25/85 (29.41%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H   ++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  639 LHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQH 723          

HSP 5 Score: 56.6102 bits (135), Expect = 1.506e-9
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  265 IETKTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 359          

HSP 6 Score: 55.4546 bits (132), Expect = 3.913e-9
Identity = 25/83 (30.12%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH+  V  LL++  +     +    P+H A + G +++ ELL++      A  ++ + P+H+A  H   DIVKLL+
Sbjct:  507 LHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 589          

HSP 7 Score: 55.4546 bits (132), Expect = 4.895e-9
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH N+VK LL+ N N          P+H A R+GH++ V  L++ +     + +    P+H+A K+G   + +LL++
Sbjct:  474 LHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLE 557          

HSP 8 Score: 54.6842 bits (130), Expect = 8.529e-9
Identity = 29/83 (34.94%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K H++++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  342 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 424          

HSP 9 Score: 53.9138 bits (128), Expect = 1.348e-8
Identity = 23/85 (27.06%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+ +
Sbjct:  375 LHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQN 459          

HSP 10 Score: 53.5286 bits (127), Expect = 1.877e-8
Identity = 26/87 (29.89%), Postives = 44/87 (50.57%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V K+LL     V A  +    P+H A R GH  +V+LL+++  +      +   P+H+A + G  + V  L++
Sbjct:  438 ETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLE 524          

HSP 11 Score: 52.7582 bits (125), Expect = 4.092e-8
Identity = 30/98 (30.61%), Postives = 53/98 (54.08%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   +H A   GHL +VK LL +  +   +      P+H A R GH ++ + L+ +K  + A  +    P+H A + G  ++VKLL+++
Sbjct:  397 IDAVTESGLT--PLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLEN 492          

HSP 12 Score: 51.6026 bits (122), Expect = 1.127e-7
Identity = 30/98 (30.61%), Postives = 50/98 (51.02%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V      P+ +A + G+    D++K++ D
Sbjct:  694 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791          

HSP 13 Score: 49.2914 bits (116), Expect = 6.967e-7
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  606 LHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH 690          
BLAST of SMED30030283 vs. UniProt/SwissProt
Match: sp|Q02357|ANK1_MOUSE (Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 4.459e-14
Identity = 29/85 (34.12%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   +E   + ++ P+H A R GH++I E+L+DH   I+A  ++ + PIH+A +    D V+LL+ +
Sbjct:  239 LHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQY 323          

HSP 2 Score: 63.1586 bits (152), Expect = 7.685e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H   ++GH+ V   L+     V+AT R    P+H A+  G+IK+V+ L+ H+ D+ A  +    P+H A + G  DIV LL+
Sbjct:  668 LHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 750          

HSP 3 Score: 62.003 bits (149), Expect = 2.401e-11
Identity = 29/85 (34.12%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  +V  T +  I P+H A+R+G++ +V LL+D    IE   +  + P+H A ++G   I ++L+DH
Sbjct:  206 LHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDH 290          

HSP 4 Score: 60.4622 bits (145), Expect = 7.576e-11
Identity = 25/85 (29.41%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH  +V  LL +  N     +  + P+H  +++GH+ + ++LI H   ++A  R    P+H+A  +G   +VK L+ H
Sbjct:  635 LHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQH 719          

HSP 5 Score: 56.6102 bits (135), Expect = 1.463e-9
Identity = 28/97 (28.87%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IE   K ++T   +H A + GH+ + + LLD    ++A  +  + PIH A +  H+  V LL+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  261 IETRTKDELT--PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLD 355          

HSP 6 Score: 56.6102 bits (135), Expect = 1.921e-9
Identity = 25/83 (30.12%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++    LL++  +     +    P+H A + G +++ ELL++H     A  ++ + P+H+A  H   DIVKLL+
Sbjct:  503 LHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLL 585          

HSP 7 Score: 55.0694 bits (131), Expect = 5.555e-9
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  V K LLD+     +       P+H A +K HI+++ELL+     I+AV  S + P+H+A   G   IVK L+
Sbjct:  338 LHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL 420          

HSP 8 Score: 54.299 bits (129), Expect = 1.259e-8
Identity = 23/83 (27.71%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+ H+ V++ LL    +++A     + P+H A+  GH+ IV+ L+         +  +  P+H+A + G  ++ K L+
Sbjct:  371 LHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 453          

HSP 9 Score: 51.6026 bits (122), Expect = 9.455e-8
Identity = 30/98 (30.61%), Postives = 51/98 (52.04%), Query Frame = 1
Query:   25 VDVTER----SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGF---PDIVKLLID 297
            VD T R     +H A   G++ +VKFLL    +V A  +    P+H+A ++GH  IV LL+ +      V  +   P+ +A + G+    D++K++ D
Sbjct:  690 VDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 787          

HSP 10 Score: 49.6766 bits (117), Expect = 5.354e-7
Identity = 24/85 (28.24%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A KQ  + V + LL    +  A     + P+H A ++GH ++V LL+  + +    ++S + P+HL  + G   +  +LI H
Sbjct:  602 LHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKH 686          

HSP 11 Score: 49.2914 bits (116), Expect = 6.381e-7
Identity = 23/85 (27.06%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A + GH  +VK LL+   +          P+H A R+GH+     L++ +     + +    P+H+A K+G   + +LL++H
Sbjct:  470 LHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEH 554          
BLAST of SMED30030283 vs. UniProt/SwissProt
Match: sp|O70511|ANK3_RAT (Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE=1 SV=3)

HSP 1 Score: 68.1662 bits (165), Expect = 1.577e-13
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R+    +H A K+G+ N+VK LLD+   ++A  R  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  269 VDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 364          

HSP 2 Score: 65.4698 bits (158), Expect = 1.369e-12
Identity = 33/86 (38.37%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  N  A       P+H A +K  I+++ELL+ H   I+AV  S + PIH+A   G  +IV  L+ H
Sbjct:  378 ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHH 463          

HSP 3 Score: 64.6994 bits (156), Expect = 2.627e-12
Identity = 28/99 (28.28%), Postives = 55/99 (55.56%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            D  AV +  +   S+H A ++GH+++V  LL +N NV  + +  + P+H A ++  + + E+L++    ++A  +    P+H+ C +G   IV  L+ H
Sbjct:  664 DANAVTRQGIA--SVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQH 760          

HSP 4 Score: 63.5438 bits (153), Expect = 5.453e-12
Identity = 28/84 (33.33%), Postives = 50/84 (59.52%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A   G++NV   LL++   V+ T R  I P+H A+++G+  +V+LL+D    I+A  R  + P+H   + G   +V++L+D
Sbjct:  247 LHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 330          

HSP 5 Score: 58.5362 bits (140), Expect = 4.155e-10
Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV++L+     VEA  +    P+H + R G   IV+ L+       A   S   P+HL+ + G  D+   L+DH
Sbjct:  475 ETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDH 562          

HSP 6 Score: 55.8398 bits (133), Expect = 3.340e-9
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  N  V+      +  +H A   GH K+ ++L+D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  346 LHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKH 430          

HSP 7 Score: 55.8398 bits (133), Expect = 3.439e-9
Identity = 27/96 (28.12%), Postives = 49/96 (51.04%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            K+D   R     +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + K+L+D
Sbjct:  301 KIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD 396          

HSP 8 Score: 54.6842 bits (130), Expect = 9.196e-9
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH +V  FLLD   ++  T +    P+H A + G +++  LL+      +A  +S + P+H+A  +    +  LL+D 
Sbjct:  544 LHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 628          

HSP 9 Score: 54.299 bits (129), Expect = 9.562e-9
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++  +NV + L++Q  +V+A  +    P+H     G+IKIV  L+ H   + A  ++   P+H A + G   I+ +L+ +
Sbjct:  709 LHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 793          

HSP 10 Score: 53.5286 bits (127), Expect = 2.343e-8
Identity = 23/86 (26.74%), Postives = 51/86 (59.30%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  LL +  NV+A  +     +H A+  G  ++V++L+ +  ++ A  ++   P+++A +    ++V+ L+D+
Sbjct:   77 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDN 162          

HSP 11 Score: 52.7582 bits (125), Expect = 3.327e-8
Identity = 27/96 (28.12%), Postives = 48/96 (50.00%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +EA  K D T   +H + + G  ++V+ LL Q  +  A       P+H + R+GH  +   L+DH   +    +    P+H+A K+G  ++  LL+
Sbjct:  500 VEAKAKDDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 593          

HSP 12 Score: 52.373 bits (124), Expect = 5.362e-8
Identity = 21/85 (24.71%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  +++   LL+   +  A  R  I  +H A ++GH+ +V LL+    ++   ++S + P+HLA +    ++ ++L++ 
Sbjct:  643 LHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQ 727          

HSP 13 Score: 51.2174 bits (121), Expect = 1.535e-7
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  A  +    P+H A +K  + I   L+++  D  AV R  +  +HLA + G  D+V LL+
Sbjct:  610 LHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 692          

HSP 14 Score: 48.521 bits (114), Expect = 1.046e-6
Identity = 26/93 (27.96%), Postives = 47/93 (50.54%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLL--DQNCNVEATM------RGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  D N ++E+ M           P+H A   G+I +  LL++    ++   R+ + P+H+A K G  ++VKLL+D
Sbjct:  205 ALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLD 297          

HSP 15 Score: 47.7506 bits (112), Expect = 2.172e-6
Identity = 30/99 (30.30%), Postives = 51/99 (51.52%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   IH A   GH+N+V  L+    +   T +RG+   +H A R G  ++V  L+     +EA  +    P+H++ + G  DIV+ L+  
Sbjct:  434 IQAVTESGLT--PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGE-TALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ 529          

HSP 16 Score: 46.2098 bits (108), Expect = 8.663e-6
Identity = 22/86 (25.58%), Postives = 42/86 (48.84%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   G   VVK L+    NV A  +    P++ A ++ H+++V  L+D+             P+ +A + G   +V LL+++
Sbjct:  110 ALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN 195          
BLAST of SMED30030283 vs. UniProt/SwissProt
Match: sp|Q12955|ANK3_HUMAN (Ankyrin-3 OS=Homo sapiens OX=9606 GN=ANK3 PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 1.986e-13
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R+    +H A K+G+ N+VK LLD+   ++A  R  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  261 VDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 356          

HSP 2 Score: 66.2402 bits (160), Expect = 7.393e-13
Identity = 34/86 (39.53%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  N  A       P+H A +K  IK++ELL+ H   I+AV  S + PIH+A   G  +IV  L+ H
Sbjct:  370 ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHH 455          

HSP 3 Score: 63.929 bits (154), Expect = 3.945e-12
Identity = 31/97 (31.96%), Postives = 55/97 (56.70%), Query Frame = 1
Query:   19 DKVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +  DV  +S    +H A   G++NV   LL++   V+ T R  I P+H A+++G+  +V+LL+D    I+A  R  + P+H   + G   +V++L+D
Sbjct:  226 NNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 322          

HSP 4 Score: 63.929 bits (154), Expect = 4.935e-12
Identity = 25/86 (29.07%), Postives = 50/86 (58.14%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A ++GH+++V  LL +N NV  + +  + P+H A ++  + + E+L++    ++A  +    P+H+ C +G   IV  L+ H
Sbjct:  667 SVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQH 752          

HSP 5 Score: 58.151 bits (139), Expect = 5.185e-10
Identity = 27/88 (30.68%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV++L+     VEA  +    P+H + R G   IV+ L+       A   S   P+HL+ + G  D+   L+DH
Sbjct:  467 ETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDH 554          

HSP 6 Score: 55.8398 bits (133), Expect = 2.823e-9
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  N  V+      +  +H A   GH K+ ++L+D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  338 LHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKH 422          

HSP 7 Score: 55.4546 bits (132), Expect = 4.462e-9
Identity = 27/96 (28.12%), Postives = 49/96 (51.04%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            K+D   R     +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + K+L+D
Sbjct:  293 KIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD 388          

HSP 8 Score: 54.299 bits (129), Expect = 1.148e-8
Identity = 24/85 (28.24%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++  +NV + L++Q  +V+A  +    P+H     G+IKIV  L+ H   + A  ++   P+H A + G   I+ +L+ +
Sbjct:  701 LHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQN 785          

HSP 9 Score: 54.299 bits (129), Expect = 1.182e-8
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + ++GH +V  FLLD   ++  T +    P+H A + G +++  LL+      +A  +S + P+H+A  +    +  LL+D 
Sbjct:  536 LHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 620          

HSP 10 Score: 54.299 bits (129), Expect = 1.265e-8
Identity = 24/86 (27.91%), Postives = 51/86 (59.30%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  LL +  NV+A  +     +H A+  G  ++V++L+ +  ++ A  ++   P+++A +    ++VK L+D+
Sbjct:   77 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDN 162          

HSP 11 Score: 52.7582 bits (125), Expect = 3.878e-8
Identity = 27/96 (28.12%), Postives = 48/96 (50.00%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +EA  K D T   +H + + G  ++V+ LL Q  +  A       P+H + R+GH  +   L+DH   +    +    P+H+A K+G  ++  LL+
Sbjct:  492 VEAKAKDDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 585          

HSP 12 Score: 51.9878 bits (123), Expect = 8.372e-8
Identity = 21/85 (24.71%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  +++   LL+   +  A  R  I  +H A ++GH+ +V LL+    ++   ++S + P+HLA +    ++ ++L++ 
Sbjct:  635 LHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQ 719          

HSP 13 Score: 51.6026 bits (122), Expect = 9.319e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G+I +  LL++    ++   R+ + P+H+A K G  ++VKLL+D
Sbjct:  205 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLD 289          

HSP 14 Score: 51.2174 bits (121), Expect = 1.155e-7
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  A  +    P+H A +K  + I   L+++  D  AV R  +  +HLA + G  D+V LL+
Sbjct:  602 LHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 684          

HSP 15 Score: 47.3654 bits (111), Expect = 2.846e-6
Identity = 30/99 (30.30%), Postives = 51/99 (51.52%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   IH A   GH+N+V  L+    +   T +RG+   +H A R G  ++V  L+     +EA  +    P+H++ + G  DIV+ L+  
Sbjct:  426 IQAVTESGLT--PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGE-TALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ 521          

HSP 16 Score: 46.2098 bits (108), Expect = 6.974e-6
Identity = 22/86 (25.58%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   G   VVK L+    NV A  +    P++ A ++ H+++V+ L+D+             P+ +A + G   +V LL+++
Sbjct:  110 ALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN 195          

HSP 17 Score: 46.2098 bits (108), Expect = 7.993e-6
Identity = 24/85 (28.24%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G L V   LL ++ + +A  +  + P+H A    + K+  LL+D      A  ++   P+H+A K    DI   L+++
Sbjct:  569 LHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEY 653          
BLAST of SMED30030283 vs. UniProt/SwissProt
Match: sp|G5E8K5|ANK3_MOUSE (Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 2.058e-13
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R+    +H A K+G+ N+VK LLD+   ++A  R  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  244 VDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 339          

HSP 2 Score: 63.929 bits (154), Expect = 4.127e-12
Identity = 25/86 (29.07%), Postives = 50/86 (58.14%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S+H A ++GH+++V  LL +N NV  + +  + P+H A ++  + + E+L++    ++A  +    P+H+ C +G   IV  L+ H
Sbjct:  650 SVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQH 735          

HSP 3 Score: 63.5438 bits (153), Expect = 6.715e-12
Identity = 31/94 (32.98%), Postives = 54/94 (57.45%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DV  +S    +H A   G++NV   LL++   V+ T R  I P+H A+++G+  +V+LL+D    I+A  R  + P+H   + G   +V++L+D
Sbjct:  212 DVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLD 305          

HSP 4 Score: 62.7734 bits (151), Expect = 1.147e-11
Identity = 32/86 (37.21%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  +  A       P+H A +K  I+++ELL+ H   I+AV  S + PIH+A   G  +IV  L+ H
Sbjct:  353 ALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHH 438          

HSP 5 Score: 58.9214 bits (141), Expect = 2.464e-10
Identity = 28/88 (31.82%), Postives = 43/88 (48.86%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV++L+     VEA  +    P+H + R G   IV+ L+       A   S   P+HLA + G  D+   L+DH
Sbjct:  450 ETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDH 537          

HSP 6 Score: 56.225 bits (134), Expect = 2.527e-9
Identity = 27/96 (28.12%), Postives = 50/96 (52.08%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            K+D   R     +H   + GH  VV+ LLD++  + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + K+L+D
Sbjct:  276 KIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLD 371          

HSP 7 Score: 55.0694 bits (131), Expect = 5.145e-9
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH +V  FLLD   ++  T +    P+H A + G +++  LL+      +A  +S + P+H+A  +    +  LL+D 
Sbjct:  519 LHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 603          

HSP 8 Score: 54.6842 bits (130), Expect = 7.522e-9
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  N  V+      +  +H A   GH K+ ++L+D K    A   +   P+H+ACK     +++LL+ H
Sbjct:  321 LHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKH 405          

HSP 9 Score: 53.5286 bits (127), Expect = 1.825e-8
Identity = 28/96 (29.17%), Postives = 48/96 (50.00%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +EA  K D T   +H + + G  ++V+ LL Q  +  A       P+H A R+GH  +   L+DH   +    +    P+H+A K+G  ++  LL+
Sbjct:  475 VEAKAKDDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 568          

HSP 10 Score: 52.7582 bits (125), Expect = 3.371e-8
Identity = 23/86 (26.74%), Postives = 51/86 (59.30%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  LL +  NV+A  +     +H A+  G  ++V++L+ +  ++ A  ++   P+++A +    ++V+ L+D+
Sbjct:   60 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDN 145          

HSP 11 Score: 52.373 bits (124), Expect = 5.874e-8
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G+I +  LL++    ++   R+ + P+H+A K G  ++VKLL+D
Sbjct:  188 ALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLD 272          

HSP 12 Score: 51.9878 bits (123), Expect = 7.421e-8
Identity = 21/85 (24.71%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  +++   LL+   +  A  R  I  +H A ++GH+ +V LL+    ++   ++S + P+HLA +    ++ ++L++ 
Sbjct:  618 LHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQ 702          

HSP 13 Score: 50.8322 bits (120), Expect = 2.084e-7
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  A  +    P+H A +K  + I   L+++  D  AV R  +  +HLA + G  D+V LL+
Sbjct:  585 LHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 667          

HSP 14 Score: 50.447 bits (119), Expect = 2.167e-7
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++  +NV + L++Q  +V+A  +    P+H     G+IKIV  L+ H   + A  ++    +H A + G   I+ +L+ +
Sbjct:  684 LHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQN 768          

HSP 15 Score: 46.9802 bits (110), Expect = 3.514e-6
Identity = 30/99 (30.30%), Postives = 51/99 (51.52%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   IH A   GH+N+V  L+    +   T +RG+   +H A R G  ++V  L+     +EA  +    P+H++ + G  DIV+ L+  
Sbjct:  409 IQAVTESGLT--PIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETA-LHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQ 504          
BLAST of SMED30030283 vs. TrEMBL
Match: A0A2S7NTM7 (ANK_REP_REGION domain-containing protein OS=Rutstroemia sp. NJR-2017a BVV2 OX=2070413 GN=CJF31_00011154 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.373e-17
Identity = 42/98 (42.86%), Postives = 67/98 (68.37%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DIEA DK   T  S+H A ++GHL +V+ LLD + +++   +    P+H A++KGH++IV+LL+D+  DI+A D+    P+HLA K+   +IV+LL+D
Sbjct: 1208 DIEAADKDGWT--SLHLASQKGHLEIVELLLDFSADIKVADKDGQIPLHFASQKGHLEIVQLLLDYGTDIKAADKDGSTPLHLASKNSHLEIVQLLLD 1303          

HSP 2 Score: 76.2554 bits (186), Expect = 6.076e-14
Identity = 37/94 (39.36%), Postives = 58/94 (61.70%), Query Frame = 1
Query:   19 DKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D  D   R+ +H A + GHL +V+ LLD + +++A  +    P+  A+R GH++IV+LL+D   DIEA D +   P+HLA      +IV+LL+D
Sbjct:  887 DPKDSYRRTPLHLASESGHLEIVQLLLDHSADIKAASKYGSTPLQLASRSGHLEIVQLLLDRGADIEAADENGSTPLHLASGSSHLEIVQLLLD 980          

HSP 3 Score: 73.559 bits (179), Expect = 5.068e-13
Identity = 38/99 (38.38%), Postives = 61/99 (61.62%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +IEA +K   T  S+  A   GHL +V  LL ++ ++EA  +     +H A++KGH++IVELL+D   DI+  D+    P+H A + G  +IV+LL+D+
Sbjct: 1175 NIEAANKNRST--SLQLASGSGHLEIVLLLLGRSADIEAADKDGWTSLHLASQKGHLEIVELLLDFSADIKVADKDGQIPLHFASQKGHLEIVQLLLDY 1271          

HSP 4 Score: 69.707 bits (169), Expect = 1.108e-11
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            DI+A  K   T   +  A + GHL +V+ LLD+  ++EA       P+H A+   H++IV+LL+D    IEAV+ +   P+H A   G  +I++LL+D+
Sbjct:  918 DIKAASKYGST--PLQLASRSGHLEIVQLLLDRGADIEAADENGSTPLHLASGSSHLEIVQLLLDRGAYIEAVNENRSPPLHSASCGGHLEIIQLLLDY 1014          

HSP 5 Score: 68.1662 bits (165), Expect = 4.912e-11
Identity = 35/98 (35.71%), Postives = 56/98 (57.14%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DIEA D+   T   +H A    HL +V+ LLD+   +EA    +  P+H A+  GH++I++LL+D+  DI A        +++A   G  +IV+LL+D
Sbjct:  951 DIEAADENGST--PLHLASGSSHLEIVQLLLDRGAYIEAVNENRSPPLHSASCGGHLEIIQLLLDYGADINATGEDGWMSLYIASSSGHLEIVQLLLD 1046          

HSP 6 Score: 66.6254 bits (161), Expect = 1.611e-10
Identity = 30/84 (35.71%), Postives = 52/84 (61.90%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A   GHL +V+ LLD+  ++EA    +   +  A+  G+++ V+LL+DH  DI+A D +   P++ A   G+ +IV+LL+D
Sbjct: 1055 LHLASCSGHLEIVQLLLDRGADIEAANENRSTSLQLASGSGYLETVQLLLDHSTDIKAADENGSTPLYSASCSGYLEIVQLLLD 1138          

HSP 7 Score: 66.2402 bits (160), Expect = 1.864e-10
Identity = 36/98 (36.73%), Postives = 57/98 (58.16%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DIEA ++   T  S+  A   G+L +V+ LL +  N+EA  + +   +  A+  GH++IV LL+    DIEA D+     +HLA + G  +IV+LL+D
Sbjct: 1142 DIEAANENRST--SLQLASGSGYLEIVRLLLHRGANIEAANKNRSTSLQLASGSGHLEIVLLLLGRSADIEAADKDGWTSLHLASQKGHLEIVELLLD 1237          

HSP 8 Score: 65.855 bits (159), Expect = 2.672e-10
Identity = 33/86 (38.37%), Postives = 51/86 (59.30%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            S++ A   GHL +V+ LLD N N       +  P+H A+  GH++IV+LL+D   DIEA + +    + LA   G+ + V+LL+DH
Sbjct: 1028 SLYIASSSGHLEIVQLLLDPNEN-------RSTPLHLASCSGHLEIVQLLLDRGADIEAANENRSTSLQLASGSGYLETVQLLLDH 1106          

HSP 9 Score: 61.2326 bits (147), Expect = 1.226e-8
Identity = 33/97 (34.02%), Postives = 57/97 (58.76%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DIEA ++   T  S+  A   G+L  V+ LLD + +++A       P++ A+  G+++IV+LL+D   DIEA + +    + LA   G+ +IV+LL+
Sbjct: 1076 DIEAANENRST--SLQLASGSGYLETVQLLLDHSTDIKAADENGSTPLYSASCSGYLEIVQLLLDCSADIEAANENRSTSLQLASGSGYLEIVRLLL 1170          

HSP 10 Score: 56.6102 bits (135), Expect = 4.727e-7
Identity = 32/98 (32.65%), Postives = 52/98 (53.06%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D   + K       +H +   G    V+FLL  N + +     +  P+H A+  GH++IV+LL+DH  DI+A  +    P+ LA + G  +IV+LL+D
Sbjct:  851 DATNIQKFPKQMTGLHLSAYFGVDYAVQFLLSSN-SPDPKDSYRRTPLHLASESGHLEIVQLLLDHSADIKAASKYGSTPLQLASRSGHLEIVQLLLD 947          
BLAST of SMED30030283 vs. TrEMBL
Match: A4RX23 (ANK_REP_REGION domain-containing protein (Fragment) OS=Ostreococcus lucimarinus (strain CCE9901) OX=436017 GN=OSTLU_5133 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.635e-16
Identity = 36/89 (40.45%), Postives = 51/89 (57.30%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            D    ++H AC +GH+NVV  L+   C+VE         +H A RKGH  +VELL+D    IE++D     P+H AC  G+ D+V LL+
Sbjct:   64 DCDNNALHVACSKGHVNVVSRLIKAGCDVETRAGNGATALHLAARKGHDDVVELLLDSDMQIESLDGKGATPLHAACSGGYEDVVGLLL 152          

HSP 2 Score: 53.1434 bits (126), Expect = 3.140e-6
Identity = 30/97 (30.93%), Postives = 48/97 (49.48%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            CD+E   +      ++H A ++GH +VV+ LLD +  +E+       P+H A   G+  +V LL+    +  AVD     P  +A K G  D  KL+
Sbjct:   90 CDVET--RAGNGATALHLAARKGHDDVVELLLDSDMQIESLDGKGATPLHAACSGGYEDVVGLLLKRGANTAAVDAKGKTPRQIANKKGHDDCAKLI 184          

HSP 3 Score: 52.7582 bits (125), Expect = 4.030e-6
Identity = 27/82 (32.93%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   52 FACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
             A  +G++ +VK L+    N++A        +H A  KGH+ +V  LI   CD+E    +    +HLA + G  D+V+LL+D
Sbjct:   39 VAAAKGNVEMVKLLIKSGANLDAQDDCDNNALHVACSKGHVNVVSRLIKAGCDVETRAGNGATALHLAARKGHDDVVELLLD 120          
BLAST of SMED30030283 vs. TrEMBL
Match: A0A3D8RP44 (ANK_REP_REGION domain-containing protein OS=Coleophoma crateriformis OX=565419 GN=BP5796_06485 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.014e-15
Identity = 32/100 (32.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
             D++A D  + T   +++A ++GHL + + L+DQ  +V+A    +  P+H A+  G++++ +LL+D   +I A D     P+H A ++G+ +I +LL+DH
Sbjct:   10 ADVKAADSFERT--PLYWASEKGHLEIAQMLVDQGADVKAADAYRRTPLHEASENGNLEMAQLLVDQGANINAADSFRRTPLHEASENGYLEIAQLLVDH 107          

HSP 2 Score: 66.2402 bits (160), Expect = 1.908e-11
Identity = 30/97 (30.93%), Postives = 55/97 (56.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
             D++A D    T   +H A + G+L + + L+DQ  N+ A    +  P+H A+  G+++I +LL+DH  D++A       P+H A ++G  ++ +LL
Sbjct:   43 ADVKAADAYRRT--PLHEASENGNLEMAQLLVDQGANINAADSFRRTPLHEASENGYLEIAQLLVDHGADVKAAAFRARTPLHGATQNGHLEMAQLL 137          

HSP 3 Score: 57.7658 bits (138), Expect = 3.534e-8
Identity = 23/74 (31.08%), Postives = 46/74 (62.16%), Query Frame = 1
Query:   79 VVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            + + L+DQ  +V+A    +  P++ A+ KGH++I ++L+D   D++A D     P+H A ++G  ++ +LL+D 
Sbjct:    1 MAQLLVDQGADVKAADSFERTPLYWASEKGHLEIAQMLVDQGADVKAADAYRRTPLHEASENGNLEMAQLLVDQ 74          
BLAST of SMED30030283 vs. TrEMBL
Match: A0A182GGY8 (ANK_REP_REGION domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG009719 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.078e-15
Identity = 39/98 (39.80%), Postives = 65/98 (66.33%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +IEA+++V+ T   +HFAC  GH  VV+ L+ +  N+EA  +    P+H + R GH ++VE+LI  K +IEA+++    P+HLAC+ G  ++V++LI
Sbjct: 1423 ANIEALNQVNWT--PLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILI 1518          

HSP 2 Score: 78.5666 bits (192), Expect = 8.598e-15
Identity = 38/97 (39.18%), Postives = 64/97 (65.98%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +IEA+++ + T   +H AC+ GH  VV+ L+ +  N+EA  +    P+H A+  GH K+VE+LI  K +IEA+ +    P+HLAC++G  ++V++LI
Sbjct: 1490 NIEALNQDNWT--PLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILI 1584          

HSP 3 Score: 76.2554 bits (186), Expect = 5.788e-14
Identity = 35/98 (35.71%), Postives = 62/98 (63.27%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +IEA+ + + T   +H AC+ GH  VV+ L+    N+EA  +    P+H+A + GH ++VE+L+  K +I+A+      P+HLAC++G  ++V++LI
Sbjct: 1555 ANIEALGQENWT--PLHLACRNGHKEVVEILIRGKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILI 1650          

HSP 4 Score: 75.485 bits (184), Expect = 1.028e-13
Identity = 35/98 (35.71%), Postives = 64/98 (65.31%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +IEA+++ + T   +H AC+ GH  VV+ L+ +N N++A    K  P+H A  +GH + V++LI  K +I+A+D +   P+H AC++G  ++V++L+
Sbjct: 1258 ANIEALNQHNWT--PLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILV 1353          

HSP 5 Score: 75.0998 bits (183), Expect = 1.608e-13
Identity = 33/83 (39.76%), Postives = 55/83 (66.27%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H AC+ GH  VV+ L+ +  N++A    K  P+H A + GH ++VE+L+  K +IEA+++    P+HLAC+ G  ++VK+LI
Sbjct: 1634 LHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILVGEKANIEALNQDNWTPLHLACEKGHKEVVKILI 1716          

HSP 6 Score: 74.3294 bits (181), Expect = 3.056e-13
Identity = 36/98 (36.73%), Postives = 62/98 (63.27%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +I+A+D+   T   +H AC+ GH  VV+ L+ +  N++A    K  P+H A + GH ++VE+LI  K +I+A+      P+HLAC++G  ++V++LI
Sbjct: 1324 ANIDALDENKFT--PLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILI 1419          

HSP 7 Score: 73.559 bits (179), Expect = 6.278e-13
Identity = 35/98 (35.71%), Postives = 62/98 (63.27%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +IEA+ + + T   +H AC+ GH  VV+ L+ +  N++A    K  P+H A + GH ++VE+LI  K +I+A+      P+HLAC++G  ++V++L+
Sbjct: 1588 ANIEALTQDNWT--PLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILV 1683          

HSP 8 Score: 72.7886 bits (177), Expect = 8.999e-13
Identity = 33/83 (39.76%), Postives = 54/83 (65.06%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H AC+ GH  VV+ L+    N+EA  +    P+H A   GH ++VE+LI  K +IEA+++    P+HL+C+HG  ++V++LI
Sbjct: 1403 LHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILI 1485          

HSP 9 Score: 72.7886 bits (177), Expect = 1.072e-12
Identity = 30/83 (36.14%), Postives = 54/83 (65.06%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H AC +GH   V+ L+ +  N++A    K  P+H+A + GH ++VE+L+  K +I+A+      P+HLAC++G  ++V++LI
Sbjct: 1304 LHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILI 1386          

HSP 10 Score: 72.0182 bits (175), Expect = 2.160e-12
Identity = 35/98 (35.71%), Postives = 61/98 (62.24%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +IEA+++ + T   +H A   GH  VV+ L+ +  N+EA  +    P+H A R GH ++VE+LI  K +IEA+ +    P+H AC++G  ++V++L+
Sbjct: 1522 ANIEALNQYNYT--PLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILIRGKANIEALTQDNWTPLHKACQNGHAEVVEILV 1617          

HSP 11 Score: 71.2478 bits (173), Expect = 3.726e-12
Identity = 32/83 (38.55%), Postives = 54/83 (65.06%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H AC+ GH  VV+ L+ +  N++A    K  P+H A + GH ++VE+LI  K +IEA+++    P+H AC +G  ++V++LI
Sbjct: 1370 LHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILI 1452          

HSP 12 Score: 69.3218 bits (168), Expect = 1.987e-11
Identity = 30/83 (36.14%), Postives = 53/83 (63.86%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H AC+ GH  VV+ L+ +  N+EA  +    P+H A  KGH ++V++LI  K +I+A+ +    P+HLAC+ G  ++V++ +
Sbjct: 1667 LHLACQNGHKEVVEILVGEKANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANIDALTQKKNTPLHLACQRGHKEVVEIFL 1749          

HSP 13 Score: 68.1662 bits (165), Expect = 3.778e-11
Identity = 34/94 (36.17%), Postives = 59/94 (62.77%), Query Frame = 1
Query:   16 VDKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            V+  D  E + +H+AC  G   VV+ L+    N++A  + K  P+H A + G  ++VE+LI  K +IEA+++    P+HLAC++G  ++V++LI
Sbjct: 1194 VNAADTNEYTPLHWACISGVKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILI 1287          

HSP 14 Score: 68.1662 bits (165), Expect = 4.005e-11
Identity = 33/97 (34.02%), Postives = 62/97 (63.92%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +IEA+++ + T   +H +C+ GH  VV+ L+ +  N+EA  +    P+H A + GH ++VE+LI  + +IEA+++    P+H+A  +G   +V++LI
Sbjct: 1457 NIEALNQDNWT--PLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILI 1551          

HSP 15 Score: 67.781 bits (164), Expect = 6.907e-11
Identity = 35/98 (35.71%), Postives = 57/98 (58.16%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
             +I+A+ K   T   +HFAC+ G   VV+ L+ +  N+EA  +    P+H A + GH ++VE+LI    +I+A+      P+HLAC  G  + V++LI
Sbjct: 1225 ANIDALAKNKST--PLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILI 1320          

HSP 16 Score: 65.4698 bits (158), Expect = 3.721e-10
Identity = 31/97 (31.96%), Postives = 61/97 (62.89%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
             +IEA+++ + T   +H AC++GH  VVK L+ +  N++A  + K  P+H A ++GH ++VE+ +    +I A+D     P++LA ++G  ++ K++
Sbjct: 1687 ANIEALNQDNWT--PLHLACEKGHKEVVKILIGEKANIDALTQKKNTPLHLACQRGHKEVVEIFLRKNANIAALDDEKCTPLNLASQNGHTEVEKIM 1781          

HSP 17 Score: 62.7734 bits (151), Expect = 3.425e-9
Identity = 29/84 (34.52%), Postives = 51/84 (60.71%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H +   GH  +V+ LLD   NV A    +  P+H A   G  ++VE+LI  K +I+A+ ++   P+H AC++G  ++V++LI
Sbjct: 1171 ALHISASSGHRPIVELLLDNGANVNAADTNEYTPLHWACISGVKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILI 1254          

HSP 18 Score: 55.8398 bits (133), Expect = 9.169e-7
Identity = 28/83 (33.73%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   G +  VK L++   NV A        +H +   GH  IVELL+D+  ++ A D +   P+H AC  G  ++V++LI
Sbjct: 1139 MHIAAGDGIIEEVKRLINNGANVNAPSVAGYTALHISASSGHRPIVELLLDNGANVNAADTNEYTPLHWACISGVKEVVEILI 1221          
BLAST of SMED30030283 vs. TrEMBL
Match: J9HSB7 (AAEL017480-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AaeL_AAEL017480 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.023e-15
Identity = 37/96 (38.54%), Postives = 59/96 (61.46%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T+      +HFA + GHL VVK L+D   NV+ T   +  P+H A+R G +++V+ LID+  +++  D     P+H A ++G  ++VKLLID+
Sbjct:   35 VDTTQNKGWTPLHFASQNGHLEVVKLLIDNRANVDTTQNEEWTPLHYASRNGRLEVVKFLIDNGANVDTTDNEGWTPLHYASRNGHLEVVKLLIDN 130          

HSP 2 Score: 76.6406 bits (187), Expect = 2.584e-14
Identity = 38/96 (39.58%), Postives = 58/96 (60.42%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T+      +H+A + G L VVKFL+D   NV+ T      P+H A+R GH+++V+LLID + +++  D     P+H A   G  ++VKLLID+
Sbjct:  167 VDTTDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLIDN 262          

HSP 3 Score: 74.7146 bits (182), Expect = 1.389e-13
Identity = 32/85 (37.65%), Postives = 54/85 (63.53%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H+A   GHL VVK L+D   NV+ T      P+H A++ GH+++V+LLID++ +++        P+H A ++G  ++VK LID+
Sbjct:   13 LHYASLNGHLEVVKLLIDNGANVDTTQNKGWTPLHFASQNGHLEVVKLLIDNRANVDTTQNEEWTPLHYASRNGRLEVVKFLIDN 97          

HSP 4 Score: 73.9442 bits (180), Expect = 2.257e-13
Identity = 35/96 (36.46%), Postives = 58/96 (60.42%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T+      +H+A + G L VVKFL+D   NV+ T      P+H A+R GH+++V+LLID+  +++        P+H A ++G  ++VK +ID+
Sbjct:   68 VDTTQNEEWTPLHYASRNGRLEVVKFLIDNGANVDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTTRNEGWTPLHYASRNGRLEVVKFMIDN 163          

HSP 5 Score: 73.9442 bits (180), Expect = 2.522e-13
Identity = 34/96 (35.42%), Postives = 57/96 (59.38%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T+      +H+A + GHL VVK L+D   NV+ T      P+H A+R G +++V+ +ID+  +++  D     P+H A ++G  ++VK LID+
Sbjct:  101 VDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTTRNEGWTPLHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLIDN 196          

HSP 6 Score: 71.2478 bits (173), Expect = 2.536e-12
Identity = 31/84 (36.90%), Postives = 52/84 (61.90%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H+A + G L VVKF++D   NV+ T      P+H A+R G +++V+ LID+  +++        P+H A ++G  ++VKLLID
Sbjct:  145 LHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLID 228          

HSP 7 Score: 70.8626 bits (172), Expect = 3.429e-12
Identity = 35/96 (36.46%), Postives = 59/96 (61.46%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T+      +H+A + GHL VVK L+D   NV+ T      P+H A+  GH+++V+LLID+  +++  +      +H+A ++G  ++VKLLID+
Sbjct:  200 VDTTQNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLIDNGANVDTKNTRRPTSLHIASQNGRLEVVKLLIDN 295          

HSP 8 Score: 60.077 bits (144), Expect = 2.473e-8
Identity = 32/96 (33.33%), Postives = 56/96 (58.33%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T+      +H A   GHL VVK L+D   NV+     +   +H A++ G +++V+LLID+  +++  +      +H+A ++G  ++VKLLID+
Sbjct:  233 VDTTDNEGWTPLHDASLIGHLEVVKLLIDNGANVDTKNTRRPTSLHIASQNGRLEVVKLLIDNGANVDTKNTRGSTSLHIASRNGHLEVVKLLIDN 328          
BLAST of SMED30030283 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 66.2402 bits (160), Expect = 7.870e-14
Identity = 27/85 (31.76%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I+E+L+D    I+A  ++ + PIH+A +    D V+ L+ +
Sbjct:  215 LHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQY 299          

HSP 2 Score: 63.1586 bits (152), Expect = 1.090e-12
Identity = 29/85 (34.12%), Postives = 52/85 (61.18%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  NV  T +  I P+H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I+++L+D 
Sbjct:  182 LHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQ 266          

HSP 3 Score: 60.8474 bits (146), Expect = 5.228e-12
Identity = 32/97 (32.99%), Postives = 53/97 (54.64%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++A  K D T  S+H A + GH  +VK LL+   N  +T      P+H A R+GH + V +L+D       + +    P+H+A K+G  D+ +LL++
Sbjct:  435 VDAKAKDDQT--SLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLE 529          

HSP 4 Score: 60.4622 bits (145), Expect = 8.024e-12
Identity = 27/85 (31.76%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  + ++    R+   P+H A + G  DIV LL+ H
Sbjct:  644 LHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKH 728          

HSP 5 Score: 60.077 bits (144), Expect = 1.004e-11
Identity = 32/98 (32.65%), Postives = 55/98 (56.12%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +  +  A+      P+H A+R GH ++ E L+ +   ++A  +     +H A + G  ++VKLL++H
Sbjct:  369 LEAVTESGLT--PLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEH 464          

HSP 6 Score: 59.6918 bits (143), Expect = 1.511e-11
Identity = 28/87 (32.18%), Postives = 48/87 (55.17%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V +FLL     V+A  +     +H A R GH ++V+LL++HK +  +   +   P+H+A + G    V++L+D
Sbjct:  410 ETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLD 496          

HSP 7 Score: 58.5362 bits (140), Expect = 3.738e-11
Identity = 28/83 (33.73%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  + K LLD+             P+H A +K H+++++LL+ H   +EAV  S + P+H+A   G  +IVK+L+
Sbjct:  314 LHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILL 396          

HSP 8 Score: 58.151 bits (139), Expect = 6.142e-11
Identity = 25/85 (29.41%), Postives = 48/85 (56.47%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ HL V+  LL  + ++EA     + P+H A+  GH+ IV++L+       A +  +  P+H+A + G  ++ + L+ +
Sbjct:  347 LHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQN 431          

HSP 9 Score: 57.7658 bits (138), Expect = 7.390e-11
Identity = 25/83 (30.12%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH   V+ LLD N       +    P+H A + G + + ELL++   +  A  ++ + P+H+A  H   D+V LL+
Sbjct:  479 LHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLV 561          

HSP 10 Score: 57.3806 bits (137), Expect = 9.995e-11
Identity = 28/97 (28.87%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ +++ LLDQ   ++A  +  + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  237 IDAKTKDELT--PLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLD 331          

HSP 11 Score: 57.3806 bits (137), Expect = 1.039e-10
Identity = 26/82 (31.71%), Postives = 44/82 (53.66%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  NV    R    P+H+A ++GH  IV LL+ H      +  +    + +A + G+  ++ +L
Sbjct:  677 LHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSALAIAKRLGYISVIDVL 758          

HSP 12 Score: 55.8398 bits (133), Expect = 3.034e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  611 LHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQ 695          

HSP 13 Score: 47.3654 bits (111), Expect = 3.059e-7
Identity = 23/85 (27.06%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++ ++L+D          +   P+H+ACK     ++ LL+ H
Sbjct:  281 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKH 365          

HSP 14 Score: 46.9802 bits (110), Expect = 4.979e-7
Identity = 23/83 (27.71%), Postives = 42/83 (50.60%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VV  L+ +  +  +  R    P+H A+++  +++   L+ +     A     V P+HLA + G  D+V LLI
Sbjct:  545 LHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLI 627          

HSP 15 Score: 46.595 bits (109), Expect = 6.934e-7
Identity = 23/83 (27.71%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A KQ  + V   LL    +  A     + P+H A ++G   +V LLI  + ++   ++S + P+HL  + G   I  +L+
Sbjct:  578 LHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILV 660          

HSP 16 Score: 43.8986 bits (102), Expect = 6.328e-6
Identity = 21/85 (24.71%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G ++V + LL++  N  A  +  + P+H A    ++ +V LL+       +  R+   P+H+A K    ++   L+ +
Sbjct:  512 LHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQY 596          
BLAST of SMED30030283 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 65.4698 bits (158), Expect = 1.665e-13
Identity = 26/85 (30.59%), Postives = 55/85 (64.71%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++++E+L+D    I+A  ++ + PIH+A +    D V+ L+ +
Sbjct:  218 LHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQY 302          

HSP 2 Score: 62.003 bits (149), Expect = 2.197e-12
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V +FLL     V+A  +    P+H A R GH ++V+LL++HK + ++   +   P+H+A + G     ++L+D
Sbjct:  413 ETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLD 499          

HSP 3 Score: 60.4622 bits (145), Expect = 9.015e-12
Identity = 32/98 (32.65%), Postives = 55/98 (56.12%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +  +  A+      P+H A R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  372 LEAVTESGLT--PLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEH 467          

HSP 4 Score: 59.6918 bits (143), Expect = 1.649e-11
Identity = 27/85 (31.76%), Postives = 51/85 (60.00%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +L+V + LL++  NV  T +  I P+H A R+G++ +V LL+D    I+A  +  + P+H A ++G   ++++L+D 
Sbjct:  185 LHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQ 269          

HSP 5 Score: 58.5362 bits (140), Expect = 4.119e-11
Identity = 30/97 (30.93%), Postives = 51/97 (52.58%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++A  K D T   +H A + GH  +VK LL+   N ++       P+H A R+GH     +L+D       + +    P+H+ACK+G  D+ +LL++
Sbjct:  438 VDAKAKDDQT--PLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLE 532          

HSP 6 Score: 57.3806 bits (137), Expect = 9.704e-11
Identity = 29/97 (29.90%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ V++ LLDQ   ++A  +  + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  240 IDARTKDELT--PLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLD 334          

HSP 7 Score: 56.9954 bits (136), Expect = 1.179e-10
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  + K LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  317 LHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLL 399          

HSP 8 Score: 56.9954 bits (136), Expect = 1.325e-10
Identity = 25/83 (30.12%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH +  + LLD N       +    P+H A + G + + ELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  482 LHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 564          

HSP 9 Score: 55.8398 bits (133), Expect = 3.869e-10
Identity = 26/83 (31.33%), Postives = 46/83 (55.42%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            ++H AC  G++ +VKFLL Q  +V    R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  684 ALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 766          

HSP 10 Score: 53.1434 bits (126), Expect = 3.298e-9
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ H+  +  LL  + ++EA     + P+H A   GH+ IV+ L+       A +  +  P+H+A + G  ++ + L+ +
Sbjct:  350 LHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQN 434          

HSP 11 Score: 53.1434 bits (126), Expect = 3.496e-9
Identity = 25/85 (29.41%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V +  R     +H A   G+IK+V+ L+  +  +    R    P+H A + G  DIV LL+ H
Sbjct:  652 LHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKH 736          

HSP 12 Score: 51.2174 bits (121), Expect = 1.464e-8
Identity = 28/85 (32.94%), Postives = 47/85 (55.29%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSL--VKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R    P+H A ++  +++   L+  +C   A   SL  + P+HLA + G PD+V LLI
Sbjct:  548 LHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLL--QCGANANSESLQGITPLHLAAQEGRPDMVALLI 630          

HSP 13 Score: 47.3654 bits (111), Expect = 2.913e-7
Identity = 24/85 (28.24%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++ ++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  284 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 368          

HSP 14 Score: 45.0542 bits (105), Expect = 2.004e-6
Identity = 22/90 (24.44%), Postives = 44/90 (48.89%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVE-----ATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV       T    + P+H   ++GH+ I ++L+     + +  R     +H+AC +G   +VK L+  
Sbjct:  614 LHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQ 703          

HSP 15 Score: 44.669 bits (104), Expect = 2.582e-6
Identity = 22/85 (25.88%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL    ++E T +     +H A   G  K+V  L+ +  ++ A  +    P+++A +    ++VK L+++
Sbjct:   24 LHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLEN 108          
BLAST of SMED30030283 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 64.6994 bits (156), Expect = 2.704e-13
Identity = 32/86 (37.21%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K ++D+  N  A       P+H A +K  IK++ELL+ H   I+AV  S + PIH+A   G  +IV  L++H
Sbjct:  317 ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNH 402          

HSP 2 Score: 60.8474 bits (146), Expect = 5.268e-12
Identity = 27/85 (31.76%), Postives = 52/85 (61.18%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G+ N+VK LL++   ++A  +  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  219 LHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHH 303          

HSP 3 Score: 57.3806 bits (137), Expect = 8.703e-11
Identity = 28/94 (29.79%), Postives = 53/94 (56.38%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DV  +S    +H A   G++NV   LL++   V+   R  I P+H A+++G+  +V+LL++    I+A  +  + P+H   + G   +V++L+D
Sbjct:  176 DVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLD 269          

HSP 4 Score: 56.225 bits (134), Expect = 2.327e-10
Identity = 26/88 (29.55%), Postives = 47/88 (53.41%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VVK+L+     V+A  +    P+H ++R G  +I++ L+ H    ++   S   P+HLA + G  D+  +L+D 
Sbjct:  414 ETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQ 501          

HSP 5 Score: 54.6842 bits (130), Expect = 7.787e-10
Identity = 25/85 (29.41%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH +V   LLDQ  ++  T +    P+H A + G I++  LL+  +   +A  +S + P+H+A  +    +  LL+D 
Sbjct:  483 LHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQ 567          

HSP 6 Score: 53.9138 bits (128), Expect = 1.633e-9
Identity = 23/84 (27.38%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H+  ++ V    +  +H+A   G   + K+++D
Sbjct:  252 LHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVD 335          

HSP 7 Score: 53.5286 bits (127), Expect = 2.482e-9
Identity = 23/85 (27.06%), Postives = 48/85 (56.47%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  + +   LL+   +  A  R  I P+H A+++G++ +V LL+     I   ++S + P+HLA +    ++ ++L++H
Sbjct:  582 LHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNH 666          

HSP 8 Score: 53.1434 bits (126), Expect = 2.872e-9
Identity = 26/83 (31.33%), Postives = 43/83 (51.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  A  +    P+H A +K  ++I   L+++  D  A+ R  + P+HLA + G  D+V LL+
Sbjct:  549 LHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLM 631          

HSP 9 Score: 52.373 bits (124), Expect = 6.769e-9
Identity = 25/85 (29.41%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL     V+      +  +H A   GH K+ ++++D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  285 LHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKH 369          

HSP 10 Score: 50.0618 bits (118), Expect = 3.759e-8
Identity = 18/83 (21.69%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++G++++V  L+ ++  +    +  + P+H A ++  + + E+L++H   ++   +    P+H+AC +G   +V  L+
Sbjct:  615 LHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLL 697          

HSP 11 Score: 49.2914 bits (116), Expect = 6.679e-8
Identity = 29/99 (29.29%), Postives = 55/99 (55.56%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+AV +  +T   IH A   GH N+V  L++   +   T +RG+   +H A R G  ++V+ L+ +   ++A  +    P+H++ + G P+I++ L+ H
Sbjct:  373 IQAVTESGLT--PIHVAAFMGHENIVTQLMNHGASPNTTNVRGETA-LHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQH 468          

HSP 12 Score: 48.1358 bits (113), Expect = 1.769e-7
Identity = 22/85 (25.88%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G+I +  LL++    ++   R+ + P+H+A K G  ++VKLL++
Sbjct:  152 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLE 236          

HSP 13 Score: 46.9802 bits (110), Expect = 4.551e-7
Identity = 22/86 (25.58%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  L+     V+A  +     +H A+  G   +V+ L+ +  ++ A  ++   P+++A +    D+V+ L+D+
Sbjct:   24 ALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDN 109          

HSP 14 Score: 46.2098 bits (108), Expect = 9.358e-7
Identity = 24/96 (25.00%), Postives = 47/96 (48.96%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++A  K D T   +H + + G   +++ LL      ++T      P+H A R+GH  +  +L+D    +    +    P+H+A K+G  ++  LL+
Sbjct:  439 VDAKSKDDQT--PLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLL 532          

HSP 15 Score: 43.5134 bits (101), Expect = 8.056e-6
Identity = 22/86 (25.58%), Postives = 42/86 (48.84%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   G  +VVK L+    NV A  +    P++ A ++ H+ +V  L+D+             P+ +A + G   +V LL+++
Sbjct:   57 ALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN 142          
BLAST of SMED30030283 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 64.3142 bits (155), Expect = 3.937e-13
Identity = 31/86 (36.05%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K ++D+  N  A       P+H A +K  +K++ELL+ H   ++AV  S + PIH+A   G  +IVK L  H
Sbjct:  324 ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHH 409          

HSP 2 Score: 63.929 bits (154), Expect = 5.869e-13
Identity = 30/98 (30.61%), Postives = 59/98 (60.20%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++ + + D+T   +H A K+G+ N+VK LLD+   ++A  +  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  215 VDFMARNDIT--PLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 310          

HSP 3 Score: 60.077 bits (144), Expect = 1.248e-11
Identity = 23/85 (27.06%), Postives = 52/85 (61.18%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G +++V  LL +N NV    +  + P+H A ++  + + E+L++H  +++A  ++   P+H+AC +G   +V  L+++
Sbjct:  622 LHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLEN 706          

HSP 4 Score: 58.9214 bits (141), Expect = 2.829e-11
Identity = 26/84 (30.95%), Postives = 48/84 (57.14%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A   G++NV   LL++   V+   R  I P+H A ++G+  +V+LL+D    I+A  +  + P+H   + G   +V++L+D
Sbjct:  193 LHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLD 276          

HSP 5 Score: 55.8398 bits (133), Expect = 3.525e-10
Identity = 29/97 (29.90%), Postives = 49/97 (50.52%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            DI+A D     + ++H A + G   +V+ LL +     A       P+H A R+GH +   LL++H   + A  +    P+H+A K+G  +  KLL+
Sbjct:  447 DIKAKDD----QTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLL 539          

HSP 6 Score: 54.299 bits (129), Expect = 1.179e-9
Identity = 23/84 (27.38%), Postives = 45/84 (53.57%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + K+++D
Sbjct:  259 LHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVD 342          

HSP 7 Score: 53.9138 bits (128), Expect = 1.505e-9
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G  +VV++LL     V+   +     +H A+R G  +IV+LL+       A   S   P+HLA + G  +   LL++H
Sbjct:  421 ETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEH 508          

HSP 8 Score: 53.9138 bits (128), Expect = 1.642e-9
Identity = 25/85 (29.41%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  +  V+      +  +H A   GH K+ ++++D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  292 LHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKH 376          

HSP 9 Score: 52.7582 bits (125), Expect = 4.266e-9
Identity = 23/85 (27.06%), Postives = 46/85 (54.12%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++  + V + LL+    V+A  +    P+H A   G++K+V  L++++  I +  +S   P+H A + G   I+ +L+ H
Sbjct:  655 LHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQH 739          

HSP 10 Score: 50.447 bits (119), Expect = 2.851e-8
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH      LL+   ++ A  +    P+H A + G I+  +LL+  +   +   ++ + P+H+A  +    +  LL+DH
Sbjct:  490 LHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDH 574          

HSP 11 Score: 49.6766 bits (117), Expect = 5.920e-8
Identity = 20/82 (24.39%), Postives = 43/82 (52.44%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +V FLL+    + +  +    P+H+A ++GH  I+ +L+ H+     +  +    + +A + G+  +V  L
Sbjct:  688 LHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 769          

HSP 12 Score: 49.2914 bits (116), Expect = 8.164e-8
Identity = 25/83 (30.12%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLD   +  A  +    P+H A +K  ++I   L++   +  AV R  + P+HLA + G  D+V LL+
Sbjct:  556 LHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLL 638          

HSP 13 Score: 48.9062 bits (115), Expect = 8.826e-8
Identity = 22/85 (25.88%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  L +   LL+      A  R  I P+H A ++G + +V LL+    ++   +++ + P+HLA +     + ++L++H
Sbjct:  589 LHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNH 673          

HSP 14 Score: 48.521 bits (114), Expect = 1.538e-7
Identity = 22/86 (25.58%), Postives = 47/86 (54.65%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ VV  LL    +V+A  +     +H A+  G   +V  L+ +  ++ A  ++   P+++A +    ++V+ L++H
Sbjct:   23 ALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEH 108          

HSP 15 Score: 46.595 bits (109), Expect = 5.898e-7
Identity = 26/93 (27.96%), Postives = 48/93 (51.61%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLL--DQNCNVEATMR------GKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  D N +VE+ +           P+H A   G+I +  LL++    ++ + R+ + P+H+A K G  ++VKLL+D
Sbjct:  151 ALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLD 243          
BLAST of SMED30030283 vs. Ensembl Cavefish
Match: ENSAMXT00000017800.2 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21012912:21225385:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000017800.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 5.525e-13
Identity = 34/97 (35.05%), Postives = 54/97 (55.67%), Query Frame = 1
Query:   25 VDVTERS-----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R      +H A K+G+ N+V+ LLD+   ++A  R  + P+H A R GH   VELL++    I A  ++ + P+H+A +    + VK L+ H
Sbjct:  252 VDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQH 348          

HSP 2 Score: 62.003 bits (149), Expect = 2.755e-12
Identity = 32/88 (36.36%), Postives = 51/88 (57.95%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQ--NCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  N N  A ++    P+H A +K  +K++ELL+ +   I+A+  S + PIH+A   G  +IV LL+ +
Sbjct:  362 ALHVAAHCGHYRVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQN 449          

HSP 3 Score: 55.8398 bits (133), Expect = 3.622e-10
Identity = 27/87 (31.03%), Postives = 48/87 (55.17%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E ++H A + G + VV+ LL     V+A  R    P+H A+R G  +IV+LL+ H    +A   +   P+H++ + G  D+  +L++
Sbjct:  461 ETALHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLLE 547          

HSP 4 Score: 53.1434 bits (126), Expect = 2.747e-9
Identity = 32/99 (32.32%), Postives = 56/99 (56.57%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            I+A+ +  +T   IH A   GHLN+V  LL    + + + +RG+   +H A R G +++V  L+ +   ++A  R    P+H+A + G  +IV+LL+ H
Sbjct:  420 IQAITESGLT--PIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETA-LHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQH 515          

HSP 5 Score: 52.7582 bits (125), Expect = 3.752e-9
Identity = 22/86 (25.58%), Postives = 53/86 (61.63%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+++V+ LL++  +V++  +     +H A+  G  ++V++L+    DI A  ++   P+++A +    D+V+ L+++
Sbjct:   68 ALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLEN 153          

HSP 6 Score: 51.6026 bits (122), Expect = 1.054e-8
Identity = 24/84 (28.57%), Postives = 45/84 (53.57%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH   V+ LL++   + A  +  + P+H A +  H++ V+ L+ HK  ++ V    +  +H+A   G   + KLL+D
Sbjct:  297 LHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLD 380          

HSP 7 Score: 48.1358 bits (113), Expect = 2.056e-7
Identity = 23/83 (27.71%), Postives = 42/83 (50.60%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLD+  +  AT +    P+H A +K  ++I   L+ ++ +   + +  V P+HLA + G  D+  LL+
Sbjct:  596 LHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLL 678          

HSP 8 Score: 46.2098 bits (108), Expect = 8.119e-7
Identity = 25/89 (28.09%), Postives = 47/89 (52.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVK----PIHLACKHGFPDIVKLLIDH 300
            +H A +  H+  VK LL     V+      +  +H A   GH ++ +LL+D + +  A  R+L++    P+H+ACK     +++LL+ +
Sbjct:  330 LHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNA--RALLQNGFTPLHIACKKNRVKVMELLVKY 416          

HSP 9 Score: 44.2838 bits (103), Expect = 4.553e-6
Identity = 21/86 (24.42%), Postives = 44/86 (51.16%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDR-SLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G++ +  LL++    ++   R + + P+H+A K G  ++V+LL+D
Sbjct:  196 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLD 281          

HSP 10 Score: 43.5134 bits (101), Expect = 8.659e-6
Identity = 18/84 (21.43%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H + ++G L+V   LL+   +     +    P+H A++ G + + +LL+  +   ++  ++ + P+H+A  +    +  LL+D
Sbjct:  530 LHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLD 613          
BLAST of SMED30030283 vs. Ensembl Sea Lamprey
Match: ankrd1a (ankyrin repeat domain 1a (cardiac muscle) [Source:ZFIN;Acc:ZDB-GENE-100405-1])

HSP 1 Score: 63.5438 bits (153), Expect = 3.001e-14
Identity = 32/94 (34.04%), Postives = 55/94 (58.51%), Query Frame = 1
Query:   19 DKVDVTERS-IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D  D T R+ +H + + GHL VV+ L+D+   +E   + K   +H A+R GH+ ++E L+D    I+A D  L  P+H+A + G  ++ + LI+
Sbjct:   29 DSCDATHRTALHQSSQAGHLEVVQKLIDKGAQLENKDKLKSTAMHCASRGGHVAVLETLLDSGASIKATDSILSSPLHVAARVGQYNVAEFLIE 122          

HSP 2 Score: 56.9954 bits (136), Expect = 1.130e-11
Identity = 30/99 (30.30%), Postives = 49/99 (49.49%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
              +E  DK+  T  ++H A + GH+ V++ LLD   +++AT      P+H A R G   + E LI+    + A D+    P+H   +   P   +LLI 
Sbjct:   59 AQLENKDKLKST--AMHCASRGGHVAVLETLLDSGASIKATDSILSSPLHVAARVGQYNVAEFLIERGASVNAQDKEGDTPLHEVMRQALPKFAQLLIS 155          
BLAST of SMED30030283 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 1.150e-13
Identity = 29/88 (32.95%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV+ LL     V+A  R +  P+H A R G ++IV+LL+ H    +A   +   P+H+A + G  D+  +L++H
Sbjct:   82 ETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEH 169          

HSP 2 Score: 53.9138 bits (128), Expect = 3.872e-10
Identity = 26/95 (27.37%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +VD   R     +H A + G L +V+ LL    + +A       P+H A R+GH  +  +L++H   +    +    P+H+A K+G  ++  LL+
Sbjct:  106 QVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLL 200          

HSP 3 Score: 53.1434 bits (126), Expect = 6.060e-10
Identity = 25/83 (30.12%), Postives = 42/83 (50.60%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  AT +    P+H A +K  ++I   L+++  +  A+ R  V P+HL  + G  D+  LL+
Sbjct:  217 LHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLL 299          

HSP 4 Score: 51.9878 bits (123), Expect = 1.456e-9
Identity = 22/85 (25.88%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  L +   LL+      A  R  + P+H   ++GH  +  LL+    D+    +S + P+HLA +    ++ ++L+ H
Sbjct:  250 LHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKH 334          

HSP 5 Score: 51.9878 bits (123), Expect = 1.702e-9
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH +V   LL +  +V    +  + P+H A ++  + + E+L+ H   + A  +    P+ +AC +G   +V  L+ H
Sbjct:  283 LHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQH 367          

HSP 6 Score: 48.9062 bits (115), Expect = 2.060e-8
Identity = 21/85 (24.71%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH +V   LL+   ++  T +    P+H A++ G +++  LL+      +A  ++ + P+H+A  +    +  LL+D 
Sbjct:  151 LHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQ 235          

HSP 7 Score: 45.0542 bits (105), Expect = 4.263e-7
Identity = 23/68 (33.82%), Postives = 38/68 (55.88%), Query Frame = 1
Query:   91 LLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            LLD+  N          P+H A +K  I+++ELL+ +   IEAV  S + P+H++   G  +IV +L+
Sbjct:    1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL 68          

HSP 8 Score: 44.2838 bits (103), Expect = 8.596e-7
Identity = 27/118 (22.88%), Postives = 48/118 (40.68%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKP---------------------------------IHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+  + V++ LL    ++EA     + P                                 +H A R G  ++V  L+ +   ++A  R    P+H+A + G  +IV+LL+ H
Sbjct:   19 LHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQH 136          

HSP 9 Score: 42.743 bits (99), Expect = 2.609e-6
Identity = 19/51 (37.25%), Postives = 30/51 (58.82%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKC 207
            AC  G++ +V FLL    +V +  +    P+H+A ++GH  IV LL+  KC
Sbjct:  352 ACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL--KC 400          
BLAST of SMED30030283 vs. Ensembl Sea Lamprey
Match: ankdd1a (ankyrin repeat and death domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-070615-8])

HSP 1 Score: 63.1586 bits (152), Expect = 1.728e-13
Identity = 31/87 (35.63%), Postives = 49/87 (56.32%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQN--CNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A + GHL  V+ LLD    C++ A     +  +H A + GH +I +LLID   DI A+      P+HLA ++ +P IV  L++
Sbjct:  210 ALHLAAEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVE 296          

HSP 2 Score: 53.1434 bits (126), Expect = 5.541e-10
Identity = 28/98 (28.57%), Postives = 51/98 (52.04%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            CDI A++   +   ++H A K GH  + K L+D+  ++ A    +  P+H A +  +  IV  L++ + +I  VD      +H+A + GF +   LL+
Sbjct:  233 CDINALNNESMN--ALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAEANINLVDCRNQTALHIAAETGFQESADLLL 328          

HSP 3 Score: 45.0542 bits (105), Expect = 3.937e-7
Identity = 24/76 (31.58%), Postives = 40/76 (52.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLD--QNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHG 267
            +H A ++GH+  VK+ L+  ++  +E     +  P   A   G + ++ELL+D+KC  +  D      IHLA K G
Sbjct:  105 LHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQDVSDGEKNTAIHLAAKSG 180          
BLAST of SMED30030283 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.1586 bits (152), Expect = 2.063e-13
Identity = 29/88 (32.95%), Postives = 49/88 (55.68%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            E ++H A + G   VV+ LL     V+A  R +  P+H A R G ++IV+LL+ H    +A   +   P+H+A + G  D+  +L++H
Sbjct:  172 ETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEH 259          

HSP 2 Score: 54.6842 bits (130), Expect = 1.921e-10
Identity = 28/84 (33.33%), Postives = 45/84 (53.57%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A   GH    K LLD+  N          P+H A +K  I+++ELL+ +   IEAV  S + P+H++   G  +IV +L+
Sbjct:   75 ALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL 158          

HSP 3 Score: 53.1434 bits (126), Expect = 6.491e-10
Identity = 26/95 (27.37%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   22 KVDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +VD   R     +H A + G L +V+ LL    + +A       P+H A R+GH  +  +L++H   +    +    P+H+A K+G  ++  LL+
Sbjct:  196 QVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLL 290          

HSP 4 Score: 52.7582 bits (125), Expect = 9.584e-10
Identity = 25/83 (30.12%), Postives = 42/83 (50.60%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +  V   LLDQ  +  AT +    P+H A +K  ++I   L+++  +  A+ R  V P+HL  + G  D+  LL+
Sbjct:  307 LHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLL 389          

HSP 5 Score: 52.373 bits (124), Expect = 1.247e-9
Identity = 23/84 (27.38%), Postives = 47/84 (55.95%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  VV+ LL+Q   + A  +  + P+H AT+  H++ V++L+ ++  ++ +    +  +H+A   G     K+L+D
Sbjct:   10 LHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLD 93          

HSP 6 Score: 51.6026 bits (122), Expect = 2.172e-9
Identity = 22/85 (25.88%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+  L +   LL+      A  R  + P+H   ++GH  +  LL+    D+    +S + P+HLA +    ++ ++L+ H
Sbjct:  340 LHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKH 424          

HSP 7 Score: 51.2174 bits (121), Expect = 2.614e-9
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH +V   LL +  +V    +  + P+H A ++  + + E+L+ H   + A  +    P+ +AC +G   +V  L+ H
Sbjct:  373 LHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQH 457          

HSP 8 Score: 48.1358 bits (113), Expect = 3.104e-8
Identity = 21/85 (24.71%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++GH +V   LL+   ++  T +    P+H A++ G +++  LL+      +A  ++ + P+H+A  +    +  LL+D 
Sbjct:  241 LHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQ 325          

HSP 9 Score: 43.5134 bits (101), Expect = 1.441e-6
Identity = 21/83 (25.30%), Postives = 43/83 (51.81%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H ACK+  + V++ LL    ++EA     + P+H +   G++ IV +L+       A +      +H+A + G  ++V+ L+
Sbjct:  109 LHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLL 191          

HSP 10 Score: 42.3578 bits (98), Expect = 4.046e-6
Identity = 19/51 (37.25%), Postives = 30/51 (58.82%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKC 207
            AC  G++ +V FLL    +V +  +    P+H+A ++GH  IV LL+  KC
Sbjct:  442 ACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL--KC 490          
BLAST of SMED30030283 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002048.1 (pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 gene:ENSPMAG00000001850.1 transcript:ENSPMAT00000002048.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.003 bits (149), Expect = 4.864e-13
Identity = 33/98 (33.67%), Postives = 55/98 (56.12%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEA-VDRSLVKPIHLACKHGFPDIVKLLI 294
            DI+ +      + ++  AC  GH ++V  L+ +  N+E   +    P+  A   GH+ +VELL+D+  DIEA  +R+   P+ LAC  G  ++V+LLI
Sbjct:  923 DIDCIHTESNHDTALTLACAGGHEDLVTVLIARGANIEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLI 1020          

HSP 2 Score: 53.5286 bits (127), Expect = 3.952e-10
Identity = 33/110 (30.00%), Postives = 54/110 (49.09%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIH-----------FACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D+ AV K+    RS+H            AC  G+  + + LL  + NVE   ++G I P+  A   G + IV LL+ H  D+ A   +    +  AC  G+ D+V++L++
Sbjct:  112 DVNAVRKLLGEGRSVHETTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGVKGDITPLMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLE 221          

HSP 3 Score: 53.1434 bits (126), Expect = 7.090e-10
Identity = 35/99 (35.35%), Postives = 54/99 (54.55%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEA-TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +IE  DK   T   +  A   GH+NVV+ LLD   ++EA + R K  P+  A   G  ++VELLI    + E  + S   P+ LA   G+ +I+K+L++
Sbjct:  958 NIEHRDKKGFT--PLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLN 1054          

HSP 4 Score: 51.6026 bits (122), Expect = 2.540e-9
Identity = 29/92 (31.52%), Postives = 46/92 (50.00%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIH----LACKHGFPDIVKLLIDH 300
            + ++ +AC+ GH +V   LL    ++E    G   P+ +A R GH+  V+ LI    D   V+R+     H    LAC  G   +V+LL+ H
Sbjct:  464 DTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGAD---VNRATANNDHTVLSLACAGGHLSVVELLLAH 552          

HSP 5 Score: 45.8246 bits (107), Expect = 2.016e-7
Identity = 25/91 (27.47%), Postives = 43/91 (47.25%), Query Frame = 1
Query:   22 KVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            K D    ++  AC  GH+ V + LL+    V         P+  A   GH+++  LLI+   ++E V+     P+  A + G  ++V LL+
Sbjct:  296 KTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLL 386          

HSP 6 Score: 45.0542 bits (105), Expect = 4.844e-7
Identity = 24/87 (27.59%), Postives = 42/87 (48.28%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E ++  AC +GHL +V+FLL+   + E         +  A   GH+++  LL++    +     S   P+ LA   G  ++  LLI+
Sbjct:  268 ESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIE 354          

HSP 7 Score: 44.669 bits (104), Expect = 5.606e-7
Identity = 20/97 (20.62%), Postives = 47/97 (48.45%), Query Frame = 1
Query:    4 DIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            D+ A       + ++  A  +GH    + ++ +  +++   +    P+  A   GH+ +V+LL++   D++A D   + P+  A + G   +V+ L+
Sbjct: 1160 DVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRKITPLMAAFRKGHVKVVRYLV 1256          

HSP 8 Score: 43.8986 bits (102), Expect = 1.066e-6
Identity = 26/87 (29.89%), Postives = 45/87 (51.72%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGK--IKPIHRATRKGHIKIVELLIDHKCDIEA-VDRSLVKPIHLACKHGFPDIVKLLID 297
            +  A   G++N++K LL+    + +    K  I P+  A   GH   V+LL+D   DI A ++ +    + LAC  G  ++V LL++
Sbjct: 1037 LSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVE 1123          

HSP 9 Score: 42.743 bits (99), Expect = 2.512e-6
Identity = 22/86 (25.58%), Postives = 44/86 (51.16%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVK-PIHLACKHGFPDIVKLLID 297
            ++ +AC  G+++VV+ LL+   N+E        P+  A   GH+++  LL++    I        +  + LAC  G  ++V+ L++
Sbjct:  203 ALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLE 288          

HSP 10 Score: 41.9726 bits (97), Expect = 4.595e-6
Identity = 25/90 (27.78%), Postives = 43/90 (47.78%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEA-VDRSLVKPIHLACKHGFPDIVKLLI 294
            D  E  +  A   GH+ +   L+++  N+E        P+  A R+GH ++V LL+    +I A  + +    + LAC  GF ++   LI
Sbjct:  331 DSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLI 420          

HSP 11 Score: 41.9726 bits (97), Expect = 4.595e-6
Identity = 25/80 (31.25%), Postives = 37/80 (46.25%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            A ++GHL +VK+LL    NV AT       +  A   GH  + +LL+    D+E        P+  A + G    V+ LI
Sbjct:  437 AAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLI 516          

HSP 12 Score: 41.9726 bits (97), Expect = 5.115e-6
Identity = 23/89 (25.84%), Postives = 45/89 (50.56%), Query Frame = 1
Query:   28 DVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +  E ++  AC  G L V  FL+    ++E    G   P+  A ++GH+++V+ L+    ++ A   +    +  AC++G  D+  LL+
Sbjct:  398 ETQETALTLACCGGFLEVADFLIKAGADLEL---GCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLL 483          
BLAST of SMED30030283 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672])

HSP 1 Score: 41.9726 bits (97), Expect = 2.828e-6
Identity = 27/86 (31.40%), Postives = 41/86 (47.67%), Query Frame = 1
Query:    7 IEAVDKVDVTER--SIHFACKQGHLNVVKFLLDQNCNVEATMRG-KIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLA 255
            +E  +  D TE    +H+A     L+VV FL+ Q  +V A        P+H A R G++ IV+ L+ H  D    D      +HL+
Sbjct:   98 LEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLS 183          
BLAST of SMED30030283 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560])

HSP 1 Score: 41.5874 bits (96), Expect = 4.377e-6
Identity = 26/80 (32.50%), Postives = 40/80 (50.00%), Query Frame = 1
Query:   19 DKVDVTERSIHFACKQGHLNVVKFLLDQNCNV-EATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLA 255
            D++D     +H+AC     +V KFLL +  N  +A   G    +H A R G+I IV+LL+ H  D    D   +  +H +
Sbjct:   80 DRIDELS-GLHWACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFS 158          
BLAST of SMED30030283 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 51.6026 bits (122), Expect = 3.285e-9
Identity = 26/93 (27.96%), Postives = 48/93 (51.61%), Query Frame = 1
Query:   19 DKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            D+   +   +H A   GH  VVK LL +   ++A       P+  A  +G +++  +L+D    ++  D++   P+HLA + G  ++VKLL+ 
Sbjct:  312 DEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLS 404          

HSP 2 Score: 51.6026 bits (122), Expect = 3.517e-9
Identity = 25/82 (30.49%), Postives = 47/82 (57.32%), Query Frame = 1
Query:   55 ACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            A  +G + V   LLD++  V+ T + K  P+H A+R+GH+++V+LL+  K +I   D +    + +A +    ++   +IDH
Sbjct:  357 AAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAIIDH 438          
BLAST of SMED30030283 vs. Ensembl Nematostella
Match: EDO45485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSW0])

HSP 1 Score: 48.1358 bits (113), Expect = 4.240e-9
Identity = 21/59 (35.59%), Postives = 34/59 (57.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAV 222
            +H A   GH  +VK LLD+  +VEA  +   +P+H A + GH  I+E+L+      +A+
Sbjct:    5 LHIAVVYGHWEIVKLLLDEGADVEAQTKNGYQPLHLAAQYGHKIIIEILLQRGAPPDAL 63          

HSP 2 Score: 46.2098 bits (108), Expect = 3.032e-8
Identity = 20/52 (38.46%), Postives = 35/52 (67.31%), Query Frame = 1
Query:  142 PIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            P+H A   GH +IV+LL+D   D+EA  ++  +P+HLA ++G   I+++L+ 
Sbjct:    4 PLHIAVVYGHWEIVKLLLDEGADVEAQTKNGYQPLHLAAQYGHKIIIEILLQ 55          
BLAST of SMED30030283 vs. Ensembl Nematostella
Match: EDO34034 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQH2])

HSP 1 Score: 49.2914 bits (116), Expect = 1.027e-8
Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKG-HIKIVELLIDHKCDIEAVDRSLVKPIHLACKH-GFPDIVKLLI 294
            ++H A  +GH++V K LL    NV AT      P+H A R G  ++   LL+ +  +I A+      P+HLA    G    +KLL+
Sbjct:   34 ALHRASYEGHVDVAKVLLTYGANVMATTHDGWHPLHSACRWGKGVEAASLLLQNGANINALTNGKQTPLHLAALGIGGTATLKLLL 119          
BLAST of SMED30030283 vs. Ensembl Nematostella
Match: EDO34704 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNN1])

HSP 1 Score: 47.7506 bits (112), Expect = 1.588e-8
Identity = 27/85 (31.76%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKC--DIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    ++  V+ LL++  NV      +  P+H A R G I ++ LL++      +  +D++   P+HLA KHG  DIV  L+
Sbjct:   12 LHCAAHAQNVEAVELLLERRANVNVLDDREETPLHAAVRTGKIAVINLLLEGSNVRMVNWLDKNERTPLHLAAKHGHVDIVDFLL 96          
BLAST of SMED30030283 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35])

HSP 1 Score: 46.9802 bits (110), Expect = 5.865e-8
Identity = 31/91 (34.07%), Postives = 47/91 (51.65%), Query Frame = 1
Query:   40 RSIHFACKQGHLNVVKFLLDQNCNVEA----TMRGKIKPIHRATRKGHIK-IVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            R IH A   GH   V+ LL    +  A    T+R  + P+H A R GH+   V +L++   D+EAV R   + IH+A  +G   ++  L+D
Sbjct:   32 RPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIR-NMTPLHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLD 121          

HSP 2 Score: 46.9802 bits (110), Expect = 6.115e-8
Identity = 26/84 (30.95%), Postives = 48/84 (57.14%), Query Frame = 1
Query:   46 IHFACKQGHLN-VVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEA--VDRSLVKPIHLACKHGFPDIVKL 288
            +H+A + GHL+  V+ L+++  ++EA  R   + IH A+  G +K++  L+D   DIE+   +R L   +H+A + G   +  L
Sbjct:   70 LHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLDAGVDIESKTYERRLT-AVHVAARQGQVTMTTL 152          

HSP 3 Score: 41.9726 bits (97), Expect = 5.097e-6
Identity = 22/88 (25.00%), Postives = 42/88 (47.73%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVK---PIHLACKHGFPD-IVKLLID 297
            +H+A + G+   ++ LL     +    R   +PIH A   GH + V +L+ +     A     ++   P+H A + G  D  V++L++
Sbjct:    1 LHYAARYGNEKCIELLLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLME 88          
BLAST of SMED30030283 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 68.5514 bits (166), Expect = 1.315e-14
Identity = 27/85 (31.76%), Postives = 56/85 (65.88%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G++ +V+ LLD+   ++A  + ++ P+H A R GH++I+E+L++H   I+A  ++ + PIH+A +    D V+ L+ +
Sbjct:  241 LHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQY 325          

HSP 2 Score: 65.4698 bits (158), Expect = 1.484e-13
Identity = 29/87 (33.33%), Postives = 50/87 (57.47%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V +FLL     V+A  +    P+H A R GH ++V+LL+DHK + ++   +   P+H+A + G    +++L+D
Sbjct:  436 ETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLD 522          

HSP 3 Score: 63.929 bits (154), Expect = 4.728e-13
Identity = 33/98 (33.67%), Postives = 56/98 (57.14%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +EAV +  +T   +H A   GHLN+VK LL +  +  A+      P+H A+R GH ++ + L+ +   ++A  +    P+H A + G  ++VKLL+DH
Sbjct:  395 LEAVTESGLT--PLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDH 490          

HSP 4 Score: 62.7734 bits (151), Expect = 1.340e-12
Identity = 27/85 (31.76%), Postives = 53/85 (62.35%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +++V + LL++  NV  T +  I P+H A+R+G++ +V LL+D    I+A  +  + P+H A ++G   I+++L++H
Sbjct:  208 LHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEH 292          

HSP 5 Score: 59.3066 bits (142), Expect = 2.008e-11
Identity = 31/97 (31.96%), Postives = 52/97 (53.61%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++A  K D T   +H A + GH  +VK LLD   N ++       P+H A R+GHI  + +L+D       + +    P+H+A K+G  D+ +LL++
Sbjct:  461 VDARAKDDQT--PLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLE 555          

HSP 6 Score: 59.3066 bits (142), Expect = 2.008e-11
Identity = 28/85 (32.94%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H   ++GH+ +   L+ Q  +V A  R    P+H A   G+IK+V+ L+  +  + A  R    P+H A + G  DIV LL+ H
Sbjct:  670 LHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKH 754          

HSP 7 Score: 58.151 bits (139), Expect = 4.967e-11
Identity = 24/83 (28.92%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH++ ++ LLD         +    P+H A++ G + + ELL++   +  A  ++ + P+H+A  H   D+VKLL+
Sbjct:  505 LHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV 587          

HSP 8 Score: 58.151 bits (139), Expect = 5.214e-11
Identity = 27/82 (32.93%), Postives = 46/82 (56.10%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            +H AC  G++ +VKFLL Q  +V A  R    P+H+A ++GH  IV LL+ H      +  +   P+ +A + G+  ++ +L
Sbjct:  703 LHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVL 784          

HSP 9 Score: 57.7658 bits (138), Expect = 8.403e-11
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  + K LLD+     A       P+H A +K H++ ++LL+ H   +EAV  S + P+H+A   G  +IVK L+
Sbjct:  340 LHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLL 422          

HSP 10 Score: 54.299 bits (129), Expect = 1.078e-9
Identity = 23/85 (27.06%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I + L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  637 LHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 721          

HSP 11 Score: 54.299 bits (129), Expect = 1.416e-9
Identity = 26/97 (26.80%), Postives = 53/97 (54.64%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            I+A  K ++T   +H A + GH+ +++ LL+    ++A  +  + PIH A +  H+  V  L+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  263 IDAQTKDELT--PLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLD 357          

HSP 12 Score: 52.373 bits (124), Expect = 6.664e-9
Identity = 24/83 (28.92%), Postives = 44/83 (53.01%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VVK L+ +  +  +T R    P+H A ++  I++  +L+ +           + P+HLA + G PD+V +LI
Sbjct:  571 LHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLI 653          

HSP 13 Score: 48.1358 bits (113), Expect = 1.692e-7
Identity = 24/85 (28.24%), Postives = 42/85 (49.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ V+ LL  N  ++      + P+H A   GH ++ ++L+D      A   +   P+H+ACK      + LL+ H
Sbjct:  307 IHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKH 391          

HSP 14 Score: 46.595 bits (109), Expect = 5.773e-7
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+GH+ +V  LL     +EAT +     +H A   G  K+V  L+++  ++ A       P+++A +    ++VK L+++
Sbjct:   47 LHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLEN 131          

HSP 15 Score: 45.8246 bits (107), Expect = 1.131e-6
Identity = 21/85 (24.71%), Postives = 44/85 (51.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K G ++V + LL++  N  A  +  + P+H A    ++ +V+LL+       +  R+   P+H+A K    ++  +L+ +
Sbjct:  538 LHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQN 622          
BLAST of SMED30030283 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 66.2402 bits (160), Expect = 6.878e-14
Identity = 34/98 (34.69%), Postives = 56/98 (57.14%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IEAV +  +T   +H A   GHLN+VK LL +  +  A+      P+H A+R GH ++ E L+ +   ++A  +    P+H A + G  ++VKLL++H
Sbjct:  335 IEAVTESGLT--PLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEH 430          

HSP 2 Score: 65.855 bits (159), Expect = 1.210e-13
Identity = 30/87 (34.48%), Postives = 49/87 (56.32%), Query Frame = 1
Query:   37 ERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            E  +H A + GH  V +FLL     V+A  +    P+H A R GH ++V+LL++HK +  +   +   P+H+A + G    V+LL+D
Sbjct:  376 ETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLD 462          

HSP 3 Score: 62.7734 bits (151), Expect = 1.239e-12
Identity = 32/97 (32.99%), Postives = 54/97 (55.67%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++A  K D T   +H A + GH  +VK LL+   N  +T      P+H A R+GH++ V LL+D +     + +    P+H+A K+G  D+ +LL++
Sbjct:  401 VDAKAKDDQT--PLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLE 495          

HSP 4 Score: 60.8474 bits (146), Expect = 5.498e-12
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A   GH  + K LLD+     +       P+H A +K H+++++LL+ H   IEAV  S + P+H+A   G  +IVK+L+
Sbjct:  280 LHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILL 362          

HSP 5 Score: 59.6918 bits (143), Expect = 1.636e-11
Identity = 25/85 (29.41%), Postives = 48/85 (56.47%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H ACK+ HL V+  LL  + ++EA     + P+H A+  GH+ IV++L+       A +  +  P+H+A + G  ++ + L+ +
Sbjct:  313 LHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQN 397          

HSP 6 Score: 58.151 bits (139), Expect = 4.684e-11
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A    +LNV + LL++  N E T      P+H A R GH +I+E+L+D+   I+A  ++ + PIH+A +    D VK L+ +
Sbjct:  187 LHIAAHYENLNVAQLLLNRGANDELT------PLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQY 265          

HSP 7 Score: 57.7658 bits (138), Expect = 6.982e-11
Identity = 24/83 (28.92%), Postives = 45/83 (54.22%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A ++GH+  V+ LLD         +    P+H A++ G + + ELL++   +  A  ++ + P+H+A  H   D+V LL+
Sbjct:  445 LHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLV 527          

HSP 8 Score: 56.9954 bits (136), Expect = 1.598e-10
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A ++G  ++V  L+ +  NV    +  + P+H   ++GH+ I ++L+     + A  R    P+H+AC +G   +VK L+  
Sbjct:  577 LHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQ 661          

HSP 9 Score: 55.4546 bits (132), Expect = 4.110e-10
Identity = 23/84 (27.38%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH  +++ LLD    ++A  +  + PIH A +  H+  V+ L+ +  +I+ +    + P+H+A   G   + K+L+D
Sbjct:  214 LHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLD 297          

HSP 10 Score: 50.447 bits (119), Expect = 3.105e-8
Identity = 25/83 (30.12%), Postives = 41/83 (49.40%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            +H A    +L+VV  L+ +  +  +  R     +H A ++  +++   L+ H     A     V P+HLA + G PDIV LLI
Sbjct:  511 LHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLI 593          

HSP 11 Score: 49.6766 bits (117), Expect = 4.908e-8
Identity = 24/84 (28.57%), Postives = 43/84 (51.19%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            ++H A KQ  + V   LL    +  A     + P+H A+++G   IV LLI  + ++   ++S + P+HL  + G   I  +L+
Sbjct:  543 ALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILV 626          

HSP 12 Score: 49.6766 bits (117), Expect = 5.204e-8
Identity = 24/85 (28.24%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            IH A +  H++ VK LL  N  ++      + P+H A   GH ++ ++L+D      +   +   P+H+ACK     ++ LL+ H
Sbjct:  247 IHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKH 331          

HSP 13 Score: 43.5134 bits (101), Expect = 7.553e-6
Identity = 21/86 (24.42%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH+ +V  LL     +E T +     +H A   G  ++V  L+++  ++ A  +    P+++A +    ++VK L+++
Sbjct:   43 ALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLEN 128          
BLAST of SMED30030283 vs. Ensembl Medaka
Match: ENSORLT00000039903.1 (pep primary_assembly:ASM223467v1:15:18469388:18484014:-1 gene:ENSORLG00000030042.1 transcript:ENSORLT00000039903.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 4.656e-13
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K ++D+  N  A       P+H A +K  ++++ELL+ H   I+AV  S + PIH+A   G  +IV  LI+H
Sbjct:  346 ALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVSALINH 431          

HSP 2 Score: 63.1586 bits (152), Expect = 8.601e-13
Identity = 29/85 (34.12%), Postives = 52/85 (61.18%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A K+G+ N+VK LLD+   +EA  +  + P+H   R GH ++VE+L+D    I +  ++ + P+H+A +    + V+LL+ H
Sbjct:  248 LHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQH 332          

HSP 3 Score: 58.151 bits (139), Expect = 5.402e-11
Identity = 30/94 (31.91%), Postives = 52/94 (55.32%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DV  +S    +H A   G++NV   LL++   V+   R  I P+H A ++G+  +V+LL+D    IEA  +  + P+H   + G   +V++L+D
Sbjct:  205 DVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLD 298          

HSP 4 Score: 54.299 bits (129), Expect = 9.652e-10
Identity = 29/97 (29.90%), Postives = 51/97 (52.58%), Query Frame = 1
Query:    7 IEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            IEA  K  +T   +H   + GH  VV+ LLD+   + +  +  + P+H AT+  H+  V+LL+ H   ++ V    +  +H+A   G   + KL++D
Sbjct:  270 IEAKTKDGLT--PLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVD 364          

HSP 5 Score: 53.1434 bits (126), Expect = 2.874e-9
Identity = 26/85 (30.59%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H A +  HLN V+ LL  +  V+      +  +H A   GH K+ +L++D K +  A   +   P+H+ACK     +++LL+ H
Sbjct:  314 LHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLLKH 398          

HSP 6 Score: 50.8322 bits (120), Expect = 2.117e-8
Identity = 23/85 (27.06%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G+I +  LL++    ++ + R+ + P+H+A K G  ++VKLL+D
Sbjct:  181 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLD 265          
BLAST of SMED30030283 vs. Ensembl Medaka
Match: ankrd39 (ankyrin repeat domain 39 [Source:NCBI gene;Acc:101162533])

HSP 1 Score: 60.8474 bits (146), Expect = 1.280e-12
Identity = 27/86 (31.40%), Postives = 45/86 (52.33%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H++ + GHL V K LL+         RG   P+HRA   GH+ +V L++ H  D    D      +H A + G  ++ +LL++H
Sbjct:   65 ALHYSSRNGHLAVCKILLENGACASPQTRGGATPLHRAAYCGHLDVVRLILHHGADPGLCDNDGASALHKAAEQGHKEVCQLLVEH 150          

HSP 2 Score: 45.0542 bits (105), Expect = 1.101e-6
Identity = 25/97 (25.77%), Postives = 48/97 (49.48%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            + +D++D  ER I  A   G L+ VK  +D+  +           +H ++R GH+ + ++L+++        R    P+H A   G  D+V+L++ H
Sbjct:   22 QTLDEMDF-ERGIWSAAVDGDLDRVKSFIDKGTDPNLRDSSGYTALHYSSRNGHLAVCKILLENGACASPQTRGGATPLHRAAYCGHLDVVRLILHH 117          
BLAST of SMED30030283 vs. Ensembl Medaka
Match: ENSORLT00000006308.2 (pep primary_assembly:ASM223467v1:1:12516834:12530692:1 gene:ENSORLG00000005003.2 transcript:ENSORLT00000006308.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.3882 bits (150), Expect = 1.473e-12
Identity = 34/96 (35.42%), Postives = 55/96 (57.29%), Query Frame = 1
Query:   25 VDVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            VD T R+    +H A K+G+ N+V  LLD+   ++A  R  + P+H A R GH   VELL++    I A  ++ + P+H++ +    + VKLL+ H
Sbjct:  207 VDFTARNGITPLHVASKRGNTNMVALLLDRGAQIDAKTRDGLTPLHCAARSGHDPAVELLLERGAPILARTKNGLSPLHMSAQGDHIECVKLLLQH 302          

HSP 2 Score: 59.3066 bits (142), Expect = 1.721e-11
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   GH  V K LLD+  N  A       P+H A +K  +K++ELL+ +   I+A+  S + PIH+A   G  +IV LL+ +
Sbjct:  316 ALHVAAHCGHYRVTKVLLDKKANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQN 401          

HSP 3 Score: 58.5362 bits (140), Expect = 3.406e-11
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 1
Query:   28 DVTERS----IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            DV  +S    +H A   G++NV   LL++   V+ T R  I P+H A+++G+  +V LL+D    I+A  R  + P+H A + G    V+LL++
Sbjct:  175 DVQSKSGFTPLHIAAHYGNVNVSTLLLNRGAAVDFTARNGITPLHVASKRGNTNMVALLLDRGAQIDAKTRDGLTPLHCAARSGHDPAVELLLE 268          

HSP 4 Score: 48.9062 bits (115), Expect = 9.667e-8
Identity = 23/84 (27.38%), Postives = 46/84 (54.76%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            +H A + GH   V+ LL++   + A  +  + P+H + +  HI+ V+LL+ H+  ++ V    +  +H+A   G   + K+L+D
Sbjct:  251 LHCAARSGHDPAVELLLERGAPILARTKNGLSPLHMSAQGDHIECVKLLLQHQAPVDDVTLDYLTALHVAAHCGHYRVTKVLLD 334          

HSP 5 Score: 45.4394 bits (106), Expect = 1.395e-6
Identity = 21/85 (24.71%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            ++H A ++        LL  + N +   +    P+H A   G++ +  LL++    ++   R+ + P+H+A K G  ++V LL+D
Sbjct:  151 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVSTLLLNRGAAVDFTARNGITPLHVASKRGNTNMVALLLD 235          

HSP 6 Score: 45.4394 bits (106), Expect = 1.538e-6
Identity = 22/85 (25.88%), Postives = 43/85 (50.59%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            +H + +  H+  VK LL     V+      +  +H A   GH ++ ++L+D K +  A   +   P+H+ACK     +++LL+ +
Sbjct:  284 LHMSAQGDHIECVKLLLQHQAPVDDVTLDYLTALHVAAHCGHYRVTKVLLDKKANPNARALNGFTPLHIACKKNRVKVMELLVKY 368          

HSP 7 Score: 44.2838 bits (103), Expect = 3.320e-6
Identity = 20/86 (23.26%), Postives = 49/86 (56.98%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A K+GH ++V+ LL +   V++  +     +H A+  G  ++V+LL+    D+ A  ++   P+++A +    ++V+  +++
Sbjct:   23 ALHLAAKEGHKDLVEELLQRGAPVDSATKKGNTALHIASLAGQKEVVKLLVSRGADVNAQSQNGFTPLYMAAQENHLEVVRYFLEN 108          

HSP 8 Score: 43.5134 bits (101), Expect = 7.979e-6
Identity = 20/86 (23.26%), Postives = 44/86 (51.16%), Query Frame = 1
Query:   43 SIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            ++H A   G   VVK L+ +  +V A  +    P++ A ++ H+++V   ++++ +          P+ +A + G   +V LL++H
Sbjct:   56 ALHIASLAGQKEVVKLLVSRGADVNAQSQNGFTPLYMAAQENHLEVVRYFLENEGNQSIATEDGFTPLAIALQQGHNSVVSLLLEH 141          
BLAST of SMED30030283 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 201.445 bits (511), Expect = 2.000e-61
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH
Sbjct:  128 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 227          

HSP 2 Score: 114.005 bits (284), Expect = 9.564e-31
Identity = 57/100 (57.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDI AV+K       ++FAC +GHL +VK L+DQNC+ EA  +   KPIH+A R+GH+ I +LLIDHKCDIEAVD+   +PIH AC  G  DIVKLLID 
Sbjct:  869 CDINAVNKC--FRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQ 966          

HSP 3 Score: 106.686 bits (265), Expect = 3.581e-28
Identity = 54/100 (54.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDK +     IHFAC+QGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID 
Sbjct:  262 CDIEAVDKRNRC--PIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQ 359          

HSP 4 Score: 105.916 bits (263), Expect = 6.477e-28
Identity = 55/100 (55.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIE+ D  +  +R IH AC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S   PIH+AC+HG  DIVKLLID 
Sbjct:  295 CDIESAD--NSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQ 392          

HSP 5 Score: 103.219 bits (256), Expect = 6.415e-27
Identity = 56/100 (56.00%), Postives = 69/100 (69.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVD+  V  + IH ACK G  ++VK L+D NC++EA      +PIH A  +G + IV+LLIDHKCDIEAVD+    PIH AC+ G  DIVKLLID 
Sbjct:  196 CDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQ 293          

HSP 6 Score: 102.064 bits (253), Expect = 1.329e-26
Identity = 50/100 (50.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIE+ D  +  +R I+ ACK+GHL +VK L++Q+C++E+      +PIH A  +GH ++V+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID 
Sbjct:  534 CDIESAD--NSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQ 631          

HSP 7 Score: 102.064 bits (253), Expect = 1.465e-26
Identity = 55/103 (53.40%), Postives = 76/103 (73.79%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGK-IKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDH 300
            C+IEA  +   ++R+IHFAC++GHL +VK L+DQ+C++EA  R K IKPI  A R GH++IV+LL+ HKCDIEAVD+  V  + IH ACK G  ++VK L+D 
Sbjct:   60 CNIEAC-RYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQ 161          

HSP 8 Score: 101.679 bits (252), Expect = 2.059e-26
Identity = 53/100 (53.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA  +     R IHFAC++G L++VK L+D  C++EA  +    PIH A  +GH  IV+LLID KCDIE+ D S  +PIH+AC+ G  DIVKLLID 
Sbjct:  229 CDIEA--RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQ 326          

HSP 9 Score: 100.523 bits (249), Expect = 5.284e-26
Identity = 53/101 (52.48%), Postives = 72/101 (71.29%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD--RSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGHL++VK L+DQ C++E+    K +PIH A  K H++IV+LLI   CDIEA +  + L  PIHLACK+G  +IVKLLI+
Sbjct:  600 CDIESAD--NSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLIN 698          

HSP 10 Score: 99.7525 bits (247), Expect = 8.506e-26
Identity = 52/100 (52.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA       PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID 
Sbjct:  328 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQ 425          

HSP 11 Score: 99.7525 bits (247), Expect = 1.106e-25
Identity = 49/98 (50.00%), Postives = 68/98 (69.39%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            CDIE+ D  +  +R IH AC QGH  VVK L+DQ+C++E+      +PI+ A ++GH+ IV+LLID KCDIE+ D    +PIH+AC+    +IVKLLI
Sbjct:  567 CDIESAD--NCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLI 662          

HSP 12 Score: 98.2117 bits (243), Expect = 3.584e-25
Identity = 53/102 (51.96%), Postives = 68/102 (66.67%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAV-DRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA +K       IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+  R   +PIH AC+ G  DIVKLLIDH
Sbjct:  666 CDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDH 767          

HSP 13 Score: 97.8265 bits (242), Expect = 5.135e-25
Identity = 49/99 (49.49%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA+       R IHFAC+QGHL++VK L+D  C++EA  +   +PIH A  K H++I ++LI+ KC+IEA D + ++PIHLAC +G  +IV LL D
Sbjct:  735 CDIEAIGG-RWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFD 832          

HSP 14 Score: 97.4413 bits (241), Expect = 5.443e-25
Identity = 47/100 (47.00%), Postives = 69/100 (69.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA    +  +R IH AC+ GH ++VK L+DQ+C++E+      +PI+ A +KGH++IV+LLI+  CDIE+ D    +PIH+AC  G  ++VKLLID 
Sbjct:  501 CDIEACK--ESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQ 598          

HSP 15 Score: 92.4337 bits (228), Expect = 3.322e-23
Identity = 45/97 (46.39%), Postives = 64/97 (65.98%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            + ++     +R IH AC++G L +VK L+DQ+C++EA      +PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  +IVKLLI+ 
Sbjct:  469 QVINVFQYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQ 565          

HSP 16 Score: 92.0485 bits (227), Expect = 4.901e-23
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDR-SLVKPIHLACKHGFPDIVKLLI 294
            CDIEA+DK +  +R IHFAC++ HL + K L+++ CN+EA     ++PIH A   GH +IV LL D KC+IE  +   L++PIH AC +G  +I+KLLI
Sbjct:  769 CDIEAMDKCN--KRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLI 865          

HSP 17 Score: 88.9669 bits (219), Expect = 6.699e-22
Identity = 46/87 (52.87%), Postives = 63/87 (72.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSL-VKPIHLACKHGFPDIVKLLIDH 300
            IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+DR   +KPI LAC++G  +IVKLL+ H
Sbjct:   40 IHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSH 126          

HSP 18 Score: 85.1149 bits (209), Expect = 1.419e-20
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            CD EAVDK +   + IH AC+QGHLN+ K L+D  C++EA  +   +PIH A  +GH  IV+LLID  CDIEA ++   +PI +A +     IV+LL
Sbjct:  902 CDTEAVDKDN--SKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 996          

HSP 19 Score: 76.6406 bits (187), Expect = 1.132e-17
Identity = 50/141 (35.46%), Postives = 71/141 (50.35%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD-------------RSLV----------------------------KPIHLACKHGFPDIVKLLIDH 300
            CDIEA    +  +  IH AC+ GH ++VK L+DQ+C++E+      +PI+ A ++GH  IV+LLID KCDIE+ D             + LV                            +PIH+AC+ G  +IVKLLID 
Sbjct:  361 CDIEACK--ESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNVQLFKTCNKSSFKYLVDVILRVQIMYICLNNVINQVINVFQYKQRPIHVACEKGKLEIVKLLIDQ 499          

HSP 20 Score: 52.7582 bits (125), Expect = 3.202e-9
Identity = 29/58 (50.00%), Postives = 40/58 (68.97%), Query Frame = 1
Query:  130 GKIKPIHRATRKGHIKIVELLIDHKCDIEAVD-RSLVKPIHLACKHGFPDIVKLLIDH 300
            G   PIH A + GH++IV+LLI+ KC+IEA   ++  + IH AC+ G  +IVKLLID 
Sbjct:   35 GLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQ 92          
BLAST of SMED30030283 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 201.06 bits (510), Expect = 2.136e-61
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH
Sbjct:  127 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 226          

HSP 2 Score: 114.005 bits (284), Expect = 1.004e-30
Identity = 57/100 (57.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDI AV+K       ++FAC +GHL +VK L+DQNC+ EA  +   KPIH+A R+GH+ I +LLIDHKCDIEAVD+   +PIH AC  G  DIVKLLID 
Sbjct:  868 CDINAVNKC--FRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQ 965          

HSP 3 Score: 106.686 bits (265), Expect = 3.723e-28
Identity = 54/100 (54.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDK +     IHFAC+QGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID 
Sbjct:  261 CDIEAVDKRNRC--PIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQ 358          

HSP 4 Score: 105.916 bits (263), Expect = 6.733e-28
Identity = 55/100 (55.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIE+ D  +  +R IH AC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S   PIH+AC+HG  DIVKLLID 
Sbjct:  294 CDIESAD--NSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQ 391          

HSP 5 Score: 103.219 bits (256), Expect = 6.866e-27
Identity = 56/100 (56.00%), Postives = 69/100 (69.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVD+  V  + IH ACK G  ++VK L+D NC++EA      +PIH A  +G + IV+LLIDHKCDIEAVD+    PIH AC+ G  DIVKLLID 
Sbjct:  195 CDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQ 292          

HSP 6 Score: 102.064 bits (253), Expect = 1.356e-26
Identity = 50/100 (50.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIE+ D  +  +R I+ ACK+GHL +VK L++Q+C++E+      +PIH A  +GH ++V+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID 
Sbjct:  533 CDIESAD--NSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQ 630          

HSP 7 Score: 102.064 bits (253), Expect = 1.508e-26
Identity = 55/103 (53.40%), Postives = 76/103 (73.79%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGK-IKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDH 300
            C+IEA  +   ++R+IHFAC++GHL +VK L+DQ+C++EA  R K IKPI  A R GH++IV+LL+ HKCDIEAVD+  V  + IH ACK G  ++VK L+D 
Sbjct:   59 CNIEAC-RYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQ 160          

HSP 8 Score: 101.679 bits (252), Expect = 2.161e-26
Identity = 53/100 (53.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA  +     R IHFAC++G L++VK L+D  C++EA  +    PIH A  +GH  IV+LLID KCDIE+ D S  +PIH+AC+ G  DIVKLLID 
Sbjct:  228 CDIEA--RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQ 325          

HSP 9 Score: 100.523 bits (249), Expect = 5.546e-26
Identity = 53/101 (52.48%), Postives = 72/101 (71.29%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD--RSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGHL++VK L+DQ C++E+    K +PIH A  K H++IV+LLI   CDIEA +  + L  PIHLACK+G  +IVKLLI+
Sbjct:  599 CDIESAD--NSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLIN 697          

HSP 10 Score: 99.7525 bits (247), Expect = 8.929e-26
Identity = 52/100 (52.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA       PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID 
Sbjct:  327 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQ 424          

HSP 11 Score: 99.3673 bits (246), Expect = 1.150e-25
Identity = 49/98 (50.00%), Postives = 68/98 (69.39%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            CDIE+ D  +  +R IH AC QGH  VVK L+DQ+C++E+      +PI+ A ++GH+ IV+LLID KCDIE+ D    +PIH+AC+    +IVKLLI
Sbjct:  566 CDIESAD--NCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLI 661          

HSP 12 Score: 98.2117 bits (243), Expect = 3.799e-25
Identity = 53/102 (51.96%), Postives = 68/102 (66.67%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAV-DRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA +K       IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+  R   +PIH AC+ G  DIVKLLIDH
Sbjct:  665 CDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDH 766          

HSP 13 Score: 97.8265 bits (242), Expect = 5.390e-25
Identity = 49/99 (49.49%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA+       R IHFAC+QGHL++VK L+D  C++EA  +   +PIH A  K H++I ++LI+ KC+IEA D + ++PIHLAC +G  +IV LL D
Sbjct:  734 CDIEAIGG-RWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFD 831          

HSP 14 Score: 97.4413 bits (241), Expect = 5.604e-25
Identity = 47/100 (47.00%), Postives = 69/100 (69.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA    +  +R IH AC+ GH ++VK L+DQ+C++E+      +PI+ A +KGH++IV+LLI+  CDIE+ D    +PIH+AC  G  ++VKLLID 
Sbjct:  500 CDIEACK--ESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQ 597          

HSP 15 Score: 92.4337 bits (228), Expect = 3.487e-23
Identity = 45/97 (46.39%), Postives = 64/97 (65.98%), Query Frame = 1
Query:   10 EAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            + ++     +R IH AC++G L +VK L+DQ+C++EA      +PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  +IVKLLI+ 
Sbjct:  468 QVINVFQYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQ 564          

HSP 16 Score: 92.0485 bits (227), Expect = 5.145e-23
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDR-SLVKPIHLACKHGFPDIVKLLI 294
            CDIEA+DK +  +R IHFAC++ HL + K L+++ CN+EA     ++PIH A   GH +IV LL D KC+IE  +   L++PIH AC +G  +I+KLLI
Sbjct:  768 CDIEAMDKCN--KRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLI 864          

HSP 17 Score: 88.9669 bits (219), Expect = 6.897e-22
Identity = 46/87 (52.87%), Postives = 63/87 (72.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSL-VKPIHLACKHGFPDIVKLLIDH 300
            IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+DR   +KPI LAC++G  +IVKLL+ H
Sbjct:   39 IHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSH 125          

HSP 18 Score: 85.1149 bits (209), Expect = 1.519e-20
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            CD EAVDK +   + IH AC+QGHLN+ K L+D  C++EA  +   +PIH A  +GH  IV+LLID  CDIEA ++   +PI +A +     IV+LL
Sbjct:  901 CDTEAVDKDN--SKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 995          

HSP 19 Score: 76.6406 bits (187), Expect = 1.177e-17
Identity = 50/141 (35.46%), Postives = 71/141 (50.35%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD-------------RSLV----------------------------KPIHLACKHGFPDIVKLLIDH 300
            CDIEA    +  +  IH AC+ GH ++VK L+DQ+C++E+      +PI+ A ++GH  IV+LLID KCDIE+ D             + LV                            +PIH+AC+ G  +IVKLLID 
Sbjct:  360 CDIEACK--ESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNVQLFKTCNKSSFKYLVDVILRVQIMYICLNNVINQVINVFQYKQRPIHVACEKGKLEIVKLLIDQ 498          

HSP 20 Score: 52.7582 bits (125), Expect = 3.330e-9
Identity = 29/58 (50.00%), Postives = 40/58 (68.97%), Query Frame = 1
Query:  130 GKIKPIHRATRKGHIKIVELLIDHKCDIEAVD-RSLVKPIHLACKHGFPDIVKLLIDH 300
            G   PIH A + GH++IV+LLI+ KC+IEA   ++  + IH AC+ G  +IVKLLID 
Sbjct:   34 GLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQ 91          
BLAST of SMED30030283 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 200.29 bits (508), Expect = 2.727e-61
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH
Sbjct:   43 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 142          

HSP 2 Score: 114.005 bits (284), Expect = 1.049e-30
Identity = 57/99 (57.58%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDI AV+K       ++FAC +GHL +VK L+DQNC+ EA  +   KPIH+A R+GH+ I +LLIDHKCDIEAVD+   +PIH AC  G  DIVKLLID
Sbjct:  776 CDINAVNKC--FRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLID 872          

HSP 3 Score: 110.923 bits (276), Expect = 1.190e-29
Identity = 56/99 (56.57%), Postives = 73/99 (73.74%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IHFAC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S  +PIH+AC+HG  DIVKLLID
Sbjct:  342 CDIESAD--NSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID 438          

HSP 4 Score: 106.686 bits (265), Expect = 3.706e-28
Identity = 54/99 (54.55%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEAVDK +     IHFAC+QGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID
Sbjct:  177 CDIEAVDKRNRC--PIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLID 273          

HSP 5 Score: 105.916 bits (263), Expect = 6.834e-28
Identity = 55/99 (55.56%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S   PIH+AC+HG  DIVKLLID
Sbjct:  210 CDIESAD--NSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLID 306          

HSP 6 Score: 103.99 bits (258), Expect = 2.769e-27
Identity = 51/99 (51.52%), Postives = 70/99 (70.71%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID
Sbjct:  309 CDIESAD--NSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLID 405          

HSP 7 Score: 102.834 bits (255), Expect = 7.387e-27
Identity = 56/99 (56.57%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEAVD+  V  + IH ACK G  ++VK L+D NC++EA      +PIH A  +G + IV+LLIDHKCDIEAVD+    PIH AC+ G  DIVKLLID
Sbjct:  111 CDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLID 207          

HSP 8 Score: 102.064 bits (253), Expect = 1.444e-26
Identity = 50/99 (50.51%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACK+GHL +VK L++Q+C++E+      +PIH A  +GH ++V+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID
Sbjct:  441 CDIESAD--NSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLID 537          

HSP 9 Score: 101.679 bits (252), Expect = 2.215e-26
Identity = 53/99 (53.54%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA  +     R IHFAC++G L++VK L+D  C++EA  +    PIH A  +GH  IV+LLID KCDIE+ D S  +PIH+AC+ G  DIVKLLID
Sbjct:  144 CDIEA--RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLID 240          

HSP 10 Score: 100.523 bits (249), Expect = 5.796e-26
Identity = 53/101 (52.48%), Postives = 72/101 (71.29%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD--RSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGHL++VK L+DQ C++E+    K +PIH A  K H++IV+LLI   CDIEA +  + L  PIHLACK+G  +IVKLLI+
Sbjct:  507 CDIESAD--NSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLIN 605          

HSP 11 Score: 100.138 bits (248), Expect = 6.705e-26
Identity = 50/99 (50.51%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA    +  +  IH AC+ GH ++VK L+DQ+C++E+      +PI+ A ++GH  IV+LLID KCDIE+ D S  +PIH AC+ G  DIVKLLID
Sbjct:  276 CDIEACK--ESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLID 372          

HSP 12 Score: 99.7525 bits (247), Expect = 9.151e-26
Identity = 52/99 (52.53%), Postives = 67/99 (67.68%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA       PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID
Sbjct:  243 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLID 339          

HSP 13 Score: 99.3673 bits (246), Expect = 1.144e-25
Identity = 49/98 (50.00%), Postives = 68/98 (69.39%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            CDIE+ D  +  +R IH AC QGH  VVK L+DQ+C++E+      +PI+ A ++GH+ IV+LLID KCDIE+ D    +PIH+AC+    +IVKLLI
Sbjct:  474 CDIESAD--NCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLI 569          

HSP 14 Score: 98.2117 bits (243), Expect = 3.300e-25
Identity = 50/99 (50.51%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA      +PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  +IVKLLI+
Sbjct:  375 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN 471          

HSP 15 Score: 97.8265 bits (242), Expect = 4.087e-25
Identity = 53/102 (51.96%), Postives = 68/102 (66.67%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAV-DRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA +K       IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+  R   +PIH AC+ G  DIVKLLIDH
Sbjct:  573 CDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDH 674          

HSP 16 Score: 97.4413 bits (241), Expect = 5.632e-25
Identity = 47/99 (47.47%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA    +  +R IH AC+ GH ++VK L+DQ+C++E+      +PI+ A +KGH++IV+LLI+  CDIE+ D    +PIH+AC  G  ++VKLLID
Sbjct:  408 CDIEACK--ESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID 504          

HSP 17 Score: 97.4413 bits (241), Expect = 5.632e-25
Identity = 49/99 (49.49%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA+       R IHFAC+QGHL++VK L+D  C++EA  +   +PIH A  K H++I ++LI+ KC+IEA D + ++PIHLAC +G  +IV LL D
Sbjct:  642 CDIEAIGG-RWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFD 739          

HSP 18 Score: 92.0485 bits (227), Expect = 5.376e-23
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDR-SLVKPIHLACKHGFPDIVKLLI 294
            CDIEA+DK +  +R IHFAC++ HL + K L+++ CN+EA     ++PIH A   GH +IV LL D KC+IE  +   L++PIH AC +G  +I+KLLI
Sbjct:  676 CDIEAMDKCN--KRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLI 772          

HSP 19 Score: 85.1149 bits (209), Expect = 1.468e-20
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            CD EAVDK +   + IH AC+QGHLN+ K L+D  C++EA  +   +PIH A  +GH  IV+LLID  CDIEA ++   +PI +A +     IV+LL
Sbjct:  809 CDTEAVDKDN--SKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 903          

HSP 20 Score: 64.3142 bits (155), Expect = 2.829e-13
Identity = 33/61 (54.10%), Postives = 44/61 (72.13%), Query Frame = 1
Query:  121 TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLV--KPIHLACKHGFPDIVKLLID 297
            T++  IKPI  A R GH++IV+LL+ HKCDIEAVD+  V  + IH ACK G  ++VK L+D
Sbjct:   15 TLQKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLD 75          
BLAST of SMED30030283 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 200.29 bits (508), Expect = 2.900e-61
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH
Sbjct:   54 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 153          

HSP 2 Score: 114.005 bits (284), Expect = 1.050e-30
Identity = 57/99 (57.58%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDI AV+K       ++FAC +GHL +VK L+DQNC+ EA  +   KPIH+A R+GH+ I +LLIDHKCDIEAVD+   +PIH AC  G  DIVKLLID
Sbjct:  787 CDINAVNKC--FRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLID 883          

HSP 3 Score: 110.923 bits (276), Expect = 1.226e-29
Identity = 56/99 (56.57%), Postives = 73/99 (73.74%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IHFAC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S  +PIH+AC+HG  DIVKLLID
Sbjct:  353 CDIESAD--NSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID 449          

HSP 4 Score: 106.686 bits (265), Expect = 3.818e-28
Identity = 54/99 (54.55%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEAVDK +     IHFAC+QGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID
Sbjct:  188 CDIEAVDKRNRC--PIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLID 284          

HSP 5 Score: 105.916 bits (263), Expect = 7.040e-28
Identity = 55/99 (55.56%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S   PIH+AC+HG  DIVKLLID
Sbjct:  221 CDIESAD--NSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLID 317          

HSP 6 Score: 103.99 bits (258), Expect = 2.797e-27
Identity = 51/99 (51.52%), Postives = 70/99 (70.71%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID
Sbjct:  320 CDIESAD--NSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLID 416          

HSP 7 Score: 102.834 bits (255), Expect = 7.391e-27
Identity = 56/99 (56.57%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEAVD+  V  + IH ACK G  ++VK L+D NC++EA      +PIH A  +G + IV+LLIDHKCDIEAVD+    PIH AC+ G  DIVKLLID
Sbjct:  122 CDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLID 218          

HSP 8 Score: 102.064 bits (253), Expect = 1.417e-26
Identity = 50/99 (50.51%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACK+GHL +VK L++Q+C++E+      +PIH A  +GH ++V+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID
Sbjct:  452 CDIESAD--NSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLID 548          

HSP 9 Score: 101.679 bits (252), Expect = 2.152e-26
Identity = 53/99 (53.54%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA  +     R IHFAC++G L++VK L+D  C++EA  +    PIH A  +GH  IV+LLID KCDIE+ D S  +PIH+AC+ G  DIVKLLID
Sbjct:  155 CDIEA--RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLID 251          

HSP 10 Score: 100.523 bits (249), Expect = 5.524e-26
Identity = 53/101 (52.48%), Postives = 72/101 (71.29%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD--RSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGHL++VK L+DQ C++E+    K +PIH A  K H++IV+LLI   CDIEA +  + L  PIHLACK+G  +IVKLLI+
Sbjct:  518 CDIESAD--NSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLIN 616          

HSP 11 Score: 100.138 bits (248), Expect = 6.516e-26
Identity = 50/99 (50.51%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA    +  +  IH AC+ GH ++VK L+DQ+C++E+      +PI+ A ++GH  IV+LLID KCDIE+ D S  +PIH AC+ G  DIVKLLID
Sbjct:  287 CDIEACK--ESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLIDQKCDIESADNSNQRPIHFACEQGHLDIVKLLID 383          

HSP 12 Score: 99.7525 bits (247), Expect = 9.068e-26
Identity = 52/99 (52.53%), Postives = 67/99 (67.68%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA       PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID
Sbjct:  254 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKQGHFDIVKLLID 350          

HSP 13 Score: 99.7525 bits (247), Expect = 1.123e-25
Identity = 49/98 (50.00%), Postives = 68/98 (69.39%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            CDIE+ D  +  +R IH AC QGH  VVK L+DQ+C++E+      +PI+ A ++GH+ IV+LLID KCDIE+ D    +PIH+AC+    +IVKLLI
Sbjct:  485 CDIESAD--NCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLI 580          

HSP 14 Score: 98.2117 bits (243), Expect = 3.270e-25
Identity = 50/99 (50.51%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA      +PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  +IVKLLI+
Sbjct:  386 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN 482          

HSP 15 Score: 97.8265 bits (242), Expect = 4.050e-25
Identity = 53/102 (51.96%), Postives = 68/102 (66.67%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAV-DRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA +K       IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+  R   +PIH AC+ G  DIVKLLIDH
Sbjct:  584 CDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDH 685          

HSP 16 Score: 97.4413 bits (241), Expect = 5.527e-25
Identity = 49/99 (49.49%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA+       R IHFAC+QGHL++VK L+D  C++EA  +   +PIH A  K H++I ++LI+ KC+IEA D + ++PIHLAC +G  +IV LL D
Sbjct:  653 CDIEAIGG-RWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFD 750          

HSP 17 Score: 97.4413 bits (241), Expect = 5.581e-25
Identity = 47/99 (47.47%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA    +  +R IH AC+ GH ++VK L+DQ+C++E+      +PI+ A +KGH++IV+LLI+  CDIE+ D    +PIH+AC  G  ++VKLLID
Sbjct:  419 CDIEACK--ESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID 515          

HSP 18 Score: 92.0485 bits (227), Expect = 5.379e-23
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDR-SLVKPIHLACKHGFPDIVKLLI 294
            CDIEA+DK +  +R IHFAC++ HL + K L+++ CN+EA     ++PIH A   GH +IV LL D KC+IE  +   L++PIH AC +G  +I+KLLI
Sbjct:  687 CDIEAMDKCN--KRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLI 783          

HSP 19 Score: 85.1149 bits (209), Expect = 1.413e-20
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            CD EAVDK +   + IH AC+QGHLN+ K L+D  C++EA  +   +PIH A  +GH  IV+LLID  CDIEA ++   +PI +A +     IV+LL
Sbjct:  820 CDTEAVDKDN--SKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 914          

HSP 20 Score: 64.6994 bits (156), Expect = 1.863e-13
Identity = 38/80 (47.50%), Postives = 51/80 (63.75%), Query Frame = 1
Query:   73 LNVVKFLLDQNCNVEA---TMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLV--KPIHLACKHGFPDIVKLLID 297
            LN+   +  Q  N E    T++  IKPI  A R GH++IV+LL+ HKCDIEAVD+  V  + IH ACK G  ++VK L+D
Sbjct:    7 LNITIMINQQFHNFEKAFHTLQKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLD 86          
BLAST of SMED30030283 vs. Planmine SMEST
Match: SMESG000017135.1 (SMESG000017135.1)

HSP 1 Score: 200.29 bits (508), Expect = 2.920e-61
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH
Sbjct:  127 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH 226          

HSP 2 Score: 114.005 bits (284), Expect = 1.071e-30
Identity = 57/99 (57.58%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDI AV+K       ++FAC +GHL +VK L+DQNC+ EA  +   KPIH+A R+GH+ I +LLIDHKCDIEAVD+   +PIH AC  G  DIVKLLID
Sbjct:  794 CDINAVNKC--FRSPVYFACSKGHLEIVKLLIDQNCDTEAVDKDNSKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLID 890          

HSP 3 Score: 106.686 bits (265), Expect = 3.709e-28
Identity = 54/99 (54.55%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEAVDK +     IHFAC+QGH ++VK L+DQ C++E+      +PIH A  +GH+ IV+LLID KCDIE+ D    +PIH+AC+ G  +IVKLLID
Sbjct:  261 CDIEAVDKRNRC--PIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLID 357          

HSP 4 Score: 105.916 bits (263), Expect = 6.841e-28
Identity = 55/99 (55.56%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC+QGHL++VK L+DQ C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S   PIH+AC+HG  DIVKLLID
Sbjct:  294 CDIESAD--NSNQRPIHVACEQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLID 390          

HSP 5 Score: 102.834 bits (255), Expect = 6.975e-27
Identity = 56/99 (56.57%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEAVD+  V  + IH ACK G  ++VK L+D NC++EA      +PIH A  +G + IV+LLIDHKCDIEAVD+    PIH AC+ G  DIVKLLID
Sbjct:  195 CDIEAVDRSLV--KPIHLACKHGFPDIVKLLIDHNCDIEARKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLID 291          

HSP 6 Score: 102.064 bits (253), Expect = 1.460e-26
Identity = 50/99 (50.51%), Postives = 71/99 (71.72%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACK+GHL +VK L++Q+C++E+      +PIH A  +GH ++V+LLID  CDIE+ D S  +PI++ACK G  DIVKLLID
Sbjct:  459 CDIESAD--NSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLID 555          

HSP 7 Score: 102.064 bits (253), Expect = 1.578e-26
Identity = 55/102 (53.92%), Postives = 76/102 (74.51%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGK-IKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLV--KPIHLACKHGFPDIVKLLID 297
            C+IEA  +   ++R+IHFAC++GHL +VK L+DQ+C++EA  R K IKPI  A R GH++IV+LL+ HKCDIEAVD+  V  + IH ACK G  ++VK L+D
Sbjct:   59 CNIEAC-RYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSHKCDIEAVDKVDVTERSIHFACKQGHLNVVKFLLD 159          

HSP 8 Score: 101.679 bits (252), Expect = 2.174e-26
Identity = 53/99 (53.54%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA  +     R IHFAC++G L++VK L+D  C++EA  +    PIH A  +GH  IV+LLID KCDIE+ D S  +PIH+AC+ G  DIVKLLID
Sbjct:  228 CDIEA--RKGCNRRPIHFACERGRLDIVKLLIDHKCDIEAVDKRNRCPIHFACEQGHFDIVKLLIDQKCDIESADNSNQRPIHVACEQGHLDIVKLLID 324          

HSP 9 Score: 100.523 bits (249), Expect = 5.580e-26
Identity = 53/101 (52.48%), Postives = 72/101 (71.29%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVD--RSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R I+ ACKQGHL++VK L+DQ C++E+    K +PIH A  K H++IV+LLI   CDIEA +  + L  PIHLACK+G  +IVKLLI+
Sbjct:  525 CDIESAD--NSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLIGQNCDIEAFNKRKGLSHPIHLACKNGHLEIVKLLIN 623          

HSP 10 Score: 99.3673 bits (246), Expect = 1.191e-25
Identity = 49/98 (50.00%), Postives = 68/98 (69.39%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLI 294
            CDIE+ D  +  +R IH AC QGH  VVK L+DQ+C++E+      +PI+ A ++GH+ IV+LLID KCDIE+ D    +PIH+AC+    +IVKLLI
Sbjct:  492 CDIESAD--NCNQRPIHVACNQGHFEVVKLLIDQHCDIESADNSNQRPIYVACKQGHLDIVKLLIDQKCDIESADNYKQRPIHVACEKNHLEIVKLLI 587          

HSP 11 Score: 99.3673 bits (246), Expect = 1.338e-25
Identity = 52/99 (52.53%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA    +  +  IH AC+ GH ++VK L+DQ+C++E+    K +PIH A  KG ++IV+LLID  CDIEA   S  +PIH+AC+HG  DIVKLLID
Sbjct:  360 CDIEACK--ESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLID 456          

HSP 12 Score: 98.2117 bits (243), Expect = 3.147e-25
Identity = 50/99 (50.51%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA      +PIH A   GH  IV+LLID  CDIE+ D S  +PI++ACK G  +IVKLLI+
Sbjct:  393 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLIN 489          

HSP 13 Score: 98.2117 bits (243), Expect = 3.785e-25
Identity = 53/102 (51.96%), Postives = 68/102 (66.67%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAV-DRSLVKPIHLACKHGFPDIVKLLIDH 300
            CDIEA +K       IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+  R   +PIH AC+ G  DIVKLLIDH
Sbjct:  591 CDIEAFNKRKGLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSERAIHFACEQGHLEIVKLLIDQHCDIEAIGGRWKNRPIHFACEQGHLDIVKLLIDH 692          

HSP 14 Score: 97.4413 bits (241), Expect = 5.423e-25
Identity = 49/99 (49.49%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA+       R IHFAC+QGHL++VK L+D  C++EA  +   +PIH A  K H++I ++LI+ KC+IEA D + ++PIHLAC +G  +IV LL D
Sbjct:  660 CDIEAIGG-RWKNRPIHFACEQGHLDIVKLLIDHKCDIEAMDKCNKRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFD 757          

HSP 15 Score: 97.4413 bits (241), Expect = 5.583e-25
Identity = 47/99 (47.47%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIEA    +  +R IH AC+ GH ++VK L+DQ+C++E+      +PI+ A +KGH++IV+LLI+  CDIE+ D    +PIH+AC  G  ++VKLLID
Sbjct:  426 CDIEACK--ESNQRPIHVACEHGHFDIVKLLIDQHCDIESADNSNQRPIYVACKKGHLEIVKLLINQHCDIESADNCNQRPIHVACNQGHFEVVKLLID 522          

HSP 16 Score: 95.9005 bits (237), Expect = 2.376e-24
Identity = 50/99 (50.51%), Postives = 66/99 (66.67%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLID 297
            CDIE+ D  +  +R IH AC++G L +VK L+DQ+C++EA       PIH A   GH  IV+LLID  CDIE+ D    +PIH+AC+ G  +IVKLLID
Sbjct:  327 CDIESAD--NYKQRPIHVACEKGKLEIVKLLIDQHCDIEACKESNQCPIHVACEHGHFDIVKLLIDQHCDIESADNYKQRPIHVACEKGKLEIVKLLID 423          

HSP 17 Score: 92.0485 bits (227), Expect = 5.277e-23
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDR-SLVKPIHLACKHGFPDIVKLLI 294
            CDIEA+DK +  +R IHFAC++ HL + K L+++ CN+EA     ++PIH A   GH +IV LL D KC+IE  +   L++PIH AC +G  +I+KLLI
Sbjct:  694 CDIEAMDKCN--KRPIHFACEKDHLEIAKVLIEKKCNIEAWDINNLRPIHLACSYGHAEIVTLLFDAKCNIEVQENIGLLRPIHFACNNGHLEIMKLLI 790          

HSP 18 Score: 88.5817 bits (218), Expect = 7.214e-22
Identity = 46/87 (52.87%), Postives = 63/87 (72.41%), Query Frame = 1
Query:   46 IHFACKQGHLNVVKFLLDQNCNVEAT-MRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSL-VKPIHLACKHGFPDIVKLLIDH 300
            IH ACK GHL +VK L+++ CN+EA   +   + IH A  +GH++IV+LLID  CDIEA+DR   +KPI LAC++G  +IVKLL+ H
Sbjct:   39 IHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLIDQHCDIEAIDRRKNIKPIDLACRNGHLEIVKLLVSH 125          

HSP 19 Score: 85.1149 bits (209), Expect = 1.499e-20
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 1
Query:    1 CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIHRATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLL 291
            CD EAVDK +   + IH AC+QGHLN+ K L+D  C++EA  +   +PIH A  +GH  IV+LLID  CDIEA ++   +PI +A +     IV+LL
Sbjct:  827 CDTEAVDKDN--SKPIHQACRQGHLNITKLLIDHKCDIEAVDKCNRRPIHFACAQGHFDIVKLLIDQNCDIEARNKWEERPIDIAKRKNQVKIVQLL 921          

HSP 20 Score: 52.7582 bits (125), Expect = 3.415e-9
Identity = 29/57 (50.88%), Postives = 40/57 (70.18%), Query Frame = 1
Query:  130 GKIKPIHRATRKGHIKIVELLIDHKCDIEAVD-RSLVKPIHLACKHGFPDIVKLLID 297
            G   PIH A + GH++IV+LLI+ KC+IEA   ++  + IH AC+ G  +IVKLLID
Sbjct:   34 GLSHPIHLACKNGHLEIVKLLINKKCNIEACRYQNSKRAIHFACEEGHLEIVKLLID 90          
The following BLAST results are available for this feature:
BLAST of SMED30030283 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK16.606e-1534.12ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK17.282e-1534.12ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK18.028e-1534.12ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK34.136e-1434.38ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK35.050e-1434.38ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
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BLAST of SMED30030283 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-442.896e-1232.29AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-442.896e-1232.29AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-443.225e-1232.29AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-443.225e-1232.29AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-444.071e-1232.29AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of SMED30030283 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank23.932e-1337.50gene:FBgn0261788 transcript:FBtr0303117[more]
Ank23.933e-1337.50gene:FBgn0261788 transcript:FBtr0303121[more]
Ank23.935e-1337.50gene:FBgn0261788 transcript:FBtr0303112[more]
Ank24.011e-1337.50gene:FBgn0261788 transcript:FBtr0303123[more]
Ank24.252e-1337.50gene:FBgn0261788 transcript:FBtr0303114[more]
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BLAST of SMED30030283 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX640476.18.953e-1533.33ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
BX640476.18.956e-1533.33ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
ank1b1.143e-1433.33ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b1.235e-1433.33ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
BX640476.14.641e-1433.33ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
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BLAST of SMED30030283 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK13.853e-1533.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK14.126e-1533.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK14.212e-1533.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK14.590e-1533.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
ANK14.597e-1533.72ankyrin 1 [Source:NCBI gene;Acc:613080][more]
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BLAST of SMED30030283 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank16.373e-1534.12ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank16.375e-1534.12ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank16.377e-1534.12ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank16.436e-1534.12ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ank16.438e-1534.12ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
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BLAST of SMED30030283 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P16157|ANK1_HUMAN3.497e-1434.12Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=... [more]
sp|Q02357|ANK1_MOUSE4.459e-1434.12Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV... [more]
sp|O70511|ANK3_RAT1.577e-1334.38Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE... [more]
sp|Q12955|ANK3_HUMAN1.986e-1334.38Ankyrin-3 OS=Homo sapiens OX=9606 GN=ANK3 PE=1 SV=... [more]
sp|G5E8K5|ANK3_MOUSE2.058e-1334.38Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV... [more]
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BLAST of SMED30030283 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2S7NTM74.373e-1742.86ANK_REP_REGION domain-containing protein OS=Rutstr... [more]
A4RX233.635e-1640.45ANK_REP_REGION domain-containing protein (Fragment... [more]
A0A3D8RP441.014e-1532.00ANK_REP_REGION domain-containing protein OS=Coleop... [more]
A0A182GGY82.078e-1539.80ANK_REP_REGION domain-containing protein OS=Aedes ... [more]
J9HSB73.023e-1538.54AAEL017480-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
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BLAST of SMED30030283 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a7.870e-1431.76ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b1.665e-1330.59ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank3b2.704e-1337.21ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
ank3a3.937e-1336.05ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ENSAMXT00000017800.25.525e-1335.05pep primary_assembly:Astyanax_mexicanus-2.0:16:210... [more]
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BLAST of SMED30030283 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankrd1a3.001e-1434.04ankyrin repeat domain 1a (cardiac muscle) [Source:... [more]
ENSPMAT00000007449.11.150e-1332.95pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
ankdd1a1.728e-1335.63ankyrin repeat and death domain containing 1A [Sou... [more]
ENSPMAT00000007446.12.063e-1332.95pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000002048.14.864e-1333.67pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 g... [more]
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BLAST of SMED30030283 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
AKR12.828e-631.40Palmitoyl transferase involved in protein palmitoy... [more]
AKR24.377e-632.50Ankyrin repeat-containing protein; member of a fam... [more]
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BLAST of SMED30030283 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO387023.285e-927.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO454854.240e-935.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO340341.027e-833.72Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347041.588e-831.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460995.865e-834.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30030283 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1b1.315e-1431.76ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
ank1a6.878e-1434.69ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ENSORLT00000039903.14.656e-1336.05pep primary_assembly:ASM223467v1:15:18469388:18484... [more]
ankrd391.280e-1231.40ankyrin repeat domain 39 [Source:NCBI gene;Acc:101... [more]
ENSORLT00000006308.21.473e-1235.42pep primary_assembly:ASM223467v1:1:12516834:125306... [more]
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BLAST of SMED30030283 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017135.12.000e-61100.00SMESG000017135.1[more]
SMESG000017135.12.136e-61100.00SMESG000017135.1[more]
SMESG000017135.12.727e-61100.00SMESG000017135.1[more]
SMESG000017135.12.900e-61100.00SMESG000017135.1[more]
SMESG000017135.12.920e-61100.00SMESG000017135.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030283 ID=SMED30030283|Name=SMED30030283|organism=Schmidtea mediterranea sexual|type=transcript|length=300bp
TGTGATATTGAGGCCGTGGATAAAGTAGATGTCACTGAGCGATCAATTCA
TTTTGCATGCAAGCAAGGTCATTTAAATGTTGTAAAATTTCTTCTAGATC
AAAATTGTAATGTTGAGGCTACGATGAGAGGAAAAATAAAACCGATTCAC
CGGGCAACGAGGAAAGGTCATATTAAAATTGTGGAACTACTTATAGATCA
TAAATGTGATATTGAGGCTGTGGATAGAAGTTTAGTCAAACCAATTCACC
TGGCATGCAAGCATGGCTTTCCAGATATTGTGAAACTGCTGATTGATCAT
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protein sequence of SMED30030283-orf-1

>SMED30030283-orf-1 ID=SMED30030283-orf-1|Name=SMED30030283-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
CDIEAVDKVDVTERSIHFACKQGHLNVVKFLLDQNCNVEATMRGKIKPIH
RATRKGHIKIVELLIDHKCDIEAVDRSLVKPIHLACKHGFPDIVKLLIDH
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008093cytoskeletal adaptor activity
GO:0019899enzyme binding
GO:0030507spectrin binding
GO:0051117ATPase binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0005886plasma membrane
GO:0014731spectrin-associated cytoskeleton
GO:0016020membrane
GO:0016529sarcoplasmic reticulum
GO:0030863cortical cytoskeleton
Vocabulary: biological process
TermDefinition
GO:0006779porphyrin-containing compound biosynthetic process
GO:0006888ER to Golgi vesicle-mediated transport
GO:0007165signal transduction
GO:0015672monovalent inorganic cation transport
GO:0048821erythrocyte development
GO:0055072iron ion homeostasis
GO:0072661protein targeting to plasma membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 44..73
e-value: 0.041
score: 23.0
coord: 11..40
e-value: 4.4
score: 16.3
IPR002110Ankyrin repeatPFAMPF13606Ank_3coord: 81..100
e-value: 0.0026
score: 18.1
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 44..76
score: 10.312
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 11..43
score: 8.63
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 13..74
e-value: 1.3E-11
score: 44.9
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 1..100
score: 25.223
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 2..100
e-value: 2.1E-26
score: 94.4
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 15..100
NoneNo IPR availablePANTHERPTHR24188ANKYRIN REPEAT PROTEINcoord: 16..99