Retrovirus-related Pol polyprotein from transposon 297

Overview
NameRetrovirus-related Pol polyprotein from transposon 297
Smed IDSMED30030244
Length (bp)474
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Retrovirus-related Pol polyprotein from transposon 297 (SMED30030244) t-SNE clustered cells

Violin plots show distribution of expression levels for Retrovirus-related Pol polyprotein from transposon 297 (SMED30030244) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030244

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30030244 dd_Smed_v4_15410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030244 dd_Smed_v4_15410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30030244 dd_Smed_v4_15410_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. TrEMBL
Match: A0A4Y2FK14 (Retrovirus-related Pol polyprotein from transposon 297 (Fragment) OS=Araneus ventricosus OX=182803 GN=pol_940 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.450e-11
Identity = 44/123 (35.77%), Postives = 65/123 (52.85%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            +F N+F  +  DI + ++ +HRI T     PIK   RR P+  +E  E +VK + +N IIE+    W S IV V  KK    RF          C+D   LN  T + ++P+P +D+ LD+LN
Sbjct:   55 EFQNLFSTSDSDIGRCNMTQHRINTG-NHPPIKQYPRRLPLAKKEEAERLVKEMVDNGIIEELSGPWASPIVLVK-KKDGSTRF----------CVDYRKLNEITIKDSYPLPRIDDTLDALN 165
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. TrEMBL
Match: A0A4Y1ZU65 (Retrovirus-related Pol polyprotein from transposon 297 OS=Araneus ventricosus OX=182803 GN=pol_3552 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.683e-11
Identity = 44/127 (34.65%), Postives = 68/127 (53.54%), Query Frame = 3 
Query:   72 KVVIQFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            +++ +F N+F  N  D+ + ++ +HRI T     PIK   RR P+  +E  E +VK +  N IIE+    W S +V V  KK    RF          C+DC  LN  T + ++P+P +D+ LD+LN
Sbjct:  200 ELLQEFQNLFSTNDSDVGRCNMTQHRINTG-NHPPIKQYPRRLPLAKKEEAERLVKEMVYNGIIEESSGPWASPVVLV-KKKDGSTRF----------CVDCRKLNEITIKDSYPLPRIDDTLDALN 314
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. TrEMBL
Match: A0A4Y2RZV5 (Retrovirus-related Pol polyprotein from transposon 297 OS=Araneus ventricosus OX=182803 GN=pol_821 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 3.225e-11
Identity = 45/123 (36.59%), Postives = 65/123 (52.85%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            +F N+F  +  DI   ++ +HRI T     PIK   RR P+  +E  E +VK + +N IIE+    W S IV V  KK    RF          C+DC  LN  T + ++P+P +D+ LD+LN
Sbjct:  273 EFQNLFSTSDSDIGGCNMTQHRINTG-NHPPIKQYPRRLPLAKKEEAERLVKEMVDNGIIEESSGPWASLIVLV-KKKDGSTRF----------CVDCRKLNEITIKDSYPLPRIDDTLDALN 383
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. TrEMBL
Match: A0A4Y1ZWJ7 (Retrovirus-related Pol polyprotein from transposon 297 (Fragment) OS=Araneus ventricosus OX=182803 GN=pol_2353 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.375e-11
Identity = 44/123 (35.77%), Postives = 66/123 (53.66%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            +F N+F  +  D+ + ++ +HRI T C   PIK   RR P+  +E  E +VK + +N IIE+    W S IV V  KK    RF          C+D   LN  T + ++P+P +D+ LD+LN
Sbjct:  187 EFQNLFSTSDSDVGRCNMTQHRINT-CNHPPIKQYPRRLPLAKKEEAERLVKEMVDNGIIEESSGPWASPIVLV-KKKDGSTRF----------CVDYRKLNEITIKDSYPLPRIDDTLDALN 297
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. TrEMBL
Match: A0A4Y2F8Z2 (Retrovirus-related Pol polyprotein from transposon 412 OS=Araneus ventricosus OX=182803 GN=POL_922 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.804e-11
Identity = 44/123 (35.77%), Postives = 66/123 (53.66%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            +F N+F  +  D+D+ ++ +HRI T     PIK   RR P+  +E  E +VK + +N IIE+    W S IV V  KK    RF          C+D   LN  T + ++P+P +D+ LD+LN
Sbjct:  236 EFQNLFSTSDSDVDRCNMTQHRINTG-NHPPIKQYPRRLPLAKKEEAERLVKEMVDNGIIEESSGPWASPIVLV-KKKDGSTRF----------CVDYRKLNEITIKDSYPLPRIDDTLDALN 346
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Cavefish
Match: ENSAMXT00000057525.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000101.1:4796788:4801347:-1 gene:ENSAMXG00000035138.1 transcript:ENSAMXT00000057525.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 1.684e-8
Identity = 38/128 (29.69%), Postives = 62/128 (48.44%), Query Frame = 3 
Query:   69 LKVVIQFTNIFMCNKWDIDKIDIFKHRIETNCGQ---IPIKSRRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSL 443
            ++++ ++  +F  +K D  K   F HRI  +  +   +P + R  P HY + +  ++  +EE +II K  S W S IV V  K   L           R C+D   LNS T + A P+P+  + L +L
Sbjct:  346 VQLIQKYEGVFSKDKLDCGKAKDFYHRIRLSDDRPFRLPYR-RVPPAHYHK-LRQVLSEMEEREIIRKSSSEWASPIVLVWKKNGDL-----------RICVDYRWLNSRTVKDAHPLPHQADCLAAL 460
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Cavefish
Match: ENSAMXT00000037150.1 (pep primary_assembly:Astyanax_mexicanus-2.0:10:22265216:22268869:1 gene:ENSAMXG00000033912.1 transcript:ENSAMXT00000037150.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.9878 bits (123), Expect = 3.919e-8
Identity = 35/122 (28.69%), Postives = 60/122 (49.18%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSL 443
            Q+ +IF   + D+    +  H I     ++P++   RR P    E ++  ++ L ++K+I    S ++S IV V  K   L           R C+D   LN+ T R A+P+P ++E LD+L
Sbjct:  294 QYQDIFAQTEGDLGCTTLLTHEIPL-LDEVPVRQPYRRIPPSQYEAVKLHIQQLLDSKVIRNSASPYSSPIVLVTKKDGSL-----------RLCVDYRQLNAKTRRDAYPLPRIEESLDAL 403
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Cavefish
Match: ENSAMXT00000054230.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:19483082:19487098:1 gene:ENSAMXG00000038338.1 transcript:ENSAMXT00000054230.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.6026 bits (122), Expect = 6.010e-8
Identity = 35/123 (28.46%), Postives = 61/123 (49.59%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSR--RQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            ++ ++F  +  D+   ++ +H I      IP++ R  R P    E ++  +  L E +++ +  S + S IV V  K   L           R C+D  LLNS T + AFP+P ++E LD+L+
Sbjct:  270 KYNSVFSAHDGDLGCTNLIEHEIPL-LDNIPVRQRHRRIPPSDYELVKAHIDQLLEAQVVRESSSPYASPIVLVKKKDGSL-----------RMCVDYRLLNSKTRKDAFPLPRIEESLDALS 380
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Cavefish
Match: ENSAMXT00000041754.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001938.1:55959:59870:1 gene:ENSAMXG00000043385.1 transcript:ENSAMXT00000041754.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 1.171e-7
Identity = 32/122 (26.23%), Postives = 62/122 (50.82%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS--RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSL 443
            +++NIF  ++ D+    +  H I     ++P++   RR P      ++  ++ L ++++I +  S ++S IV V  K   L           R C+D   LN+ T R ++P+P ++E LD+L
Sbjct:  278 KYSNIFAKSEGDLGCTSLISHEIPL-LDEVPVRQPYRRIPPSQYSTVKAHIQQLLDSRVIRESSSPFSSPIVLVTKKDGSL-----------RLCVDYRQLNAKTRRDSYPLPRIEESLDAL 387
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Cavefish
Match: ENSAMXT00000048272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000106.1:219951:223307:1 gene:ENSAMXG00000032559.1 transcript:ENSAMXT00000048272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.8322 bits (120), Expect = 1.224e-7
Identity = 34/123 (27.64%), Postives = 61/123 (49.59%), Query Frame = 3 
Query:   87 FTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIEN---IVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            + ++F     D+ +  + +H I+   G  P+K + + M +E+ IE+   I +SLE   +     SSW S IV            VR     +R C+D   LN+ T + A+P+P + + L++L+
Sbjct:   50 YGDVFSTGPTDLGRTSLVQHDIQLLPGP-PVKQQPRRMAFEKQIESDAQIQQSLESG-LASPSNSSWASPIV-----------LVRKKDQTYRLCVDYRALNARTVKDAYPLPRIQDTLETLS 159
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Medaka
Match: ENSORLT00000036827.1 (pep primary_assembly:ASM223467v1:16:28613823:28617539:1 gene:ENSORLG00000023550.1 transcript:ENSORLT00000036827.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.7734 bits (151), Expect = 8.622e-12
Identity = 37/124 (29.84%), Postives = 67/124 (54.03%), Query Frame = 3 
Query:   72 KVVIQFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSR--RQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLD 437
            KV++++ +IF  ++ ++    +  H I+T   + PIK+R  R P+ ++   ++ ++ +    IIE  +S W S +V V  KK P +RF          C+D   LN  T + ++P+P +DE LD
Sbjct:  239 KVLLEYRDIFALSEDEVGLTHLVHHEIDTGDAR-PIKTRPRRLPLAHQVAADSAIEEMLRGGIIEPSDSPWASGVVMVKKKKGPKMRF----------CVDYRPLNGVTKKDSYPLPRIDESLD 351
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Medaka
Match: ENSORLT00000044989.1 (pep primary_assembly:ASM223467v1:1:17572028:17576089:-1 gene:ENSORLG00000028051.1 transcript:ENSORLT00000044989.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 1.983e-9
Identity = 37/128 (28.91%), Postives = 64/128 (50.00%), Query Frame = 3 
Query:   72 KVVIQFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKS---RRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            K++ Q+T++F  +  D+   D  +H I      +P+     R  P  Y+E  ++I + L +  +I++  S++ S IV            VR   +  R C+D   LN+ T R AFP+P +DE  D+L+
Sbjct:  239 KLISQYTDVFALSDEDLGYADRIQHEIHLT-DDVPVTQPYRRVPPTQYKEVKDHIAQLLRKG-VIQESTSAYASPIV-----------LVRKADNSLRLCVDYRRLNAKTRRDAFPLPRIDECFDALH 353
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Medaka
Match: ENSORLT00000032171.1 (pep primary_assembly:ASM223467v1:18:2114932:2117958:1 gene:ENSORLG00000025397.1 transcript:ENSORLT00000032171.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 1.999e-7
Identity = 35/123 (28.46%), Postives = 57/123 (46.34%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSR--RQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            ++ N+F  ++ D+    +  H I       P++ R  R P    E +++ +  L  + +I +  S + S IV    K   L           R C+D   LNS T R AFP+P +DE LD+L+
Sbjct:  192 KYANVFAAHEGDLGCTTLMTHEIPL-LDDAPVRQRHRRIPPSEYEAVKDHINQLLASGVIRESNSPYASPIVLARKKDGSL-----------RMCVDYRQLNSKTRRDAFPLPRIDESLDALS 302
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Medaka
Match: ENSORLT00000041674.1 (pep primary_assembly:ASM223467v1:5:30088905:30092924:1 gene:ENSORLG00000022054.1 transcript:ENSORLT00000041674.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 2.047e-7
Identity = 35/123 (28.46%), Postives = 57/123 (46.34%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSR--RQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            ++ N+F  ++ D+    +  H I       P++ R  R P    E +++ +  L  + +I +  S + S IV    K   L           R C+D   LNS T R AFP+P +DE LD+L+
Sbjct:  284 KYANVFAAHEGDLGCTTLMTHEIPL-LDDAPVRQRHRRIPPSEYEAVKDHINQLLASGVIRESNSPYASPIVLARKKDGSL-----------RMCVDYRQLNSKTRRDAFPLPRIDESLDALS 394
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Medaka
Match: ENSORLT00000032005.1 (pep primary_assembly:ASM223467v1:7:31190450:31194463:1 gene:ENSORLG00000029990.1 transcript:ENSORLT00000032005.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.6766 bits (117), Expect = 3.114e-7
Identity = 37/130 (28.46%), Postives = 61/130 (46.92%), Query Frame = 3 
Query:   66 KLKVVIQ-FTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSR--RQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            K + ++Q + N+F  ++ D+    +  H I       P++ R  R P    E +++ +  L  + +I +  S + S IV    K   L           R C+D   LNS T R AFP+P +DE LD+L+
Sbjct:  277 KARALLQKYANVFAAHEGDLGCTTLMTHEIPL-LDDAPVRQRHRRIPPSEYEAVKDHINQLLASGVIRESNSPYASPIVLARKKDGSL-----------RMCVDYRQLNSKTRRDAFPLPPIDESLDALS 394
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Planmine SMEST
Match: SMESG000052960.1 (SMESG000052960.1)

HSP 1 Score: 139.428 bits (350), Expect = 1.199e-42
Identity = 73/121 (60.33%), Postives = 83/121 (68.60%), Query Frame = 3 
Query:   84 QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            +FT IFMCNKWDI K+DI KHRIETNCG +PIKSR+QPMHYEE +E IVK+L +NKI EK ES +NS I  V  K                T +D  +L SNT RPAF IPNVDE LDSLN
Sbjct:    5 EFTYIFMCNKWDIGKVDILKHRIETNCGPVPIKSRQQPMHYEEKLEEIVKNLAKNKITEKCESPYNSPIARVPKKG---------------TSIDFRILISNTVRPAFQIPNVDETLDSLN 110
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.201e-22
Identity = 54/118 (45.76%), Postives = 71/118 (60.17%), Query Frame = 3 
Query:   93 NIFMCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            N+FM +KWDI   ++ KH+I T    I IK RRQP++ E+ IE  +K+LE N II K  S WN+ +V V  K+K  +R           CLD   LN  T R AFP+PNVDE+LD L+
Sbjct: 2702 NVFMADKWDIGCTNLIKHKIITKGEPIMIKPRRQPINLEDKIEEAIKNLENNGIIRKCNSPWNTPLVCVWKKEKKDIRL----------CLDFRQLNKITERQAFPMPNVDEILDILH 2809
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Planmine SMEST
Match: SMESG000029866.1 (SMESG000029866.1)

HSP 1 Score: 85.1149 bits (209), Expect = 9.635e-20
Identity = 53/124 (42.74%), Postives = 78/124 (62.90%), Query Frame = 3 
Query:   72 KVVIQFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSL 443
            ++V +  +IFM N +DI K  + KH I T+C  I + SRRQP+H    IE  ++ +E+  II++F S WN+ +  VC+KKK          +  R CLD GLLN ++ RP FPIPN++E+LDS+
Sbjct:   47 RLVRKNDSIFMKNIYDIAKTQLVKHEIITSCPHIIMNSRRQPVHLLPKIEECIEEIEKYGIIKEFTSPWNTPL--VCIKKKD--------CENIRLCLDFGLLNKSSERPIFPIPNIEEILDSI 160
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Planmine SMEST
Match: SMESG000062996.1 (SMESG000062996.1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.981e-19
Identity = 47/128 (36.72%), Postives = 70/128 (54.69%), Query Frame = 3 
Query:   66 KLKVVI-QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            KL  +I QFT+IFM +++D+    +  H I+T+   I    RRQPMH E  +E +V++L E  ++ K +S WN+ +V V      +           R CLD  LLNS T + +FPIP +  +LD L 
Sbjct:   61 KLGTLISQFTSIFMASRFDLGHTKVITHEIKTSGPPILQNPRRQPMHLEGKVEELVQNLLEAGVVRKCQSPWNTPLVIVGKPDGSI-----------RMCLDFRLLNSVTEKFSFPIPEMQLLLDCLG 177
[Close]
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Planmine SMEST
Match: SMESG000062996.1 (SMESG000062996.1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.284e-18
Identity = 47/128 (36.72%), Postives = 70/128 (54.69%), Query Frame = 3 
Query:   66 KLKVVI-QFTNIFMCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIENIVKSLEENKIIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRPAFPIPNVDEMLDSLN 446
            KL  +I QFT+IFM +++D+    +  H I+T+   I    RRQPMH E  +E +V++L E  ++ K +S WN+ +V V      +           R CLD  LLNS T + +FPIP +  +LD L 
Sbjct:   75 KLGTLISQFTSIFMASRFDLGHTKVITHEIKTSGPPILQNPRRQPMHLEGKVEELVQNLLEAGVVRKCQSPWNTPLVIVGKPDGSI-----------RMCLDFRLLNSVTEKFSFPIPEMQLLLDCLG 191
The following BLAST results are available for this feature:
BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Y2FK141.450e-1135.77Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y1ZU652.683e-1134.65Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y2RZV53.225e-1136.59Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y1ZWJ73.375e-1135.77Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y2F8Z23.804e-1135.77Retrovirus-related Pol polyprotein from transposon... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000057525.11.684e-829.69pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000037150.13.919e-828.69pep primary_assembly:Astyanax_mexicanus-2.0:10:222... [more]
ENSAMXT00000054230.16.010e-828.46pep primary_assembly:Astyanax_mexicanus-2.0:14:194... [more]
ENSAMXT00000041754.11.171e-726.23pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000048272.11.224e-727.64pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000036827.18.622e-1229.84pep primary_assembly:ASM223467v1:16:28613823:28617... [more]
ENSORLT00000044989.11.983e-928.91pep primary_assembly:ASM223467v1:1:17572028:175760... [more]
ENSORLT00000032171.11.999e-728.46pep primary_assembly:ASM223467v1:18:2114932:211795... [more]
ENSORLT00000041674.12.047e-728.46pep primary_assembly:ASM223467v1:5:30088905:300929... [more]
ENSORLT00000032005.13.114e-728.46pep primary_assembly:ASM223467v1:7:31190450:311944... [more]
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BLAST of Retrovirus-related Pol polyprotein from transposon 297 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052960.11.199e-4260.33SMESG000052960.1[more]
SMESG000029259.14.201e-2245.76SMESG000029259.1[more]
SMESG000029866.19.635e-2042.74SMESG000029866.1[more]
SMESG000062996.16.981e-1936.72SMESG000062996.1[more]
SMESG000062996.11.284e-1836.72SMESG000062996.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030244 ID=SMED30030244|Name=Retrovirus-related Pol polyprotein from transposon 297|organism=Schmidtea mediterranea sexual|type=transcript|length=474bp
ATCCTCAGTTTGCAAGCAAAATTTAGCTGCAATAATAAGAAAGATAATAA
AACTGTAGATAATAAAAATTGAAAGTTGTCATACAGTTTACAAATATTTT
TATGTGTAATAAATGGGATATAGACAAAATAGATATTTTTAAACACCGAA
TAGAAACGAATTGTGGCCAAATACCAATTAAATCCCGGCGACAACCAATG
CACTATGAAGAAAATATAGAAAATATTGTTAAAAGTTTGGAGGAAAATAA
AATAATAGAGAAATTTGAATCATCTTGGAATTCCTCAATTGTGTATGTGT
GCCTAAAAAAGAAACCTCTGCTTAGATTTGTTAGACATGTTTTAGATTTG
TTTAGAACTTGTTTAGATTGTGGACTTTTAAATTCAAATACGTTTAGACC
AGCTTTTCCAATTCCTAATGTTGACGAAATGCTGGATTCTCTGAATTAGA
TCACAAAAATAGTTATTTGTTGAA
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protein sequence of SMED30030244-orf-1

>SMED30030244-orf-1 ID=SMED30030244-orf-1|Name=SMED30030244-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=116bp
MCNKWDIDKIDIFKHRIETNCGQIPIKSRRQPMHYEENIENIVKSLEENK
IIEKFESSWNSSIVYVCLKKKPLLRFVRHVLDLFRTCLDCGLLNSNTFRP
AFPIPNVDEMLDSLN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
GO:0003676nucleic acid binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0015074DNA integration