Elongation factor 2
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030239 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14
Homology
BLAST of Elongation factor 2 vs. Ensembl Human
Match: EEF2 (eukaryotic translation elongation factor 2 [Source:HGNC Symbol;Acc:HGNC:3214]) HSP 1 Score: 77.7962 bits (190), Expect = 1.681e-17 Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D + +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. Ensembl Celegans
Match: eef-2 (Elongation factor 2 [Source:UniProtKB/Swiss-Prot;Acc:P29691]) HSP 1 Score: 78.1814 bits (191), Expect = 6.100e-18 Identity = 40/75 (53.33%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P + KP V DTRKRKGL +P LD Sbjct: 774 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALD 846
BLAST of Elongation factor 2 vs. Ensembl Celegans
Match: eef-2 (Elongation factor 2 [Source:UniProtKB/Swiss-Prot;Acc:P29691]) HSP 1 Score: 78.1814 bits (191), Expect = 6.100e-18 Identity = 40/75 (53.33%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P + KP V DTRKRKGL +P LD Sbjct: 774 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALD 846
BLAST of Elongation factor 2 vs. Ensembl Celegans
Match: eef-2 (Elongation factor 2 [Source:UniProtKB/Swiss-Prot;Acc:P29691]) HSP 1 Score: 78.1814 bits (191), Expect = 6.100e-18 Identity = 40/75 (53.33%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P + KP V DTRKRKGL +P LD Sbjct: 774 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALD 846
BLAST of Elongation factor 2 vs. Ensembl Celegans
Match: eef-2 (Elongation factor 2 [Source:UniProtKB/Swiss-Prot;Acc:P29691]) HSP 1 Score: 77.7962 bits (190), Expect = 6.587e-18 Identity = 40/75 (53.33%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P + KP V DTRKRKGL +P LD Sbjct: 762 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALD 834
BLAST of Elongation factor 2 vs. Ensembl Celegans
Match: eef-2 (Elongation factor 2 [Source:UniProtKB/Swiss-Prot;Acc:P29691]) HSP 1 Score: 77.7962 bits (190), Expect = 6.587e-18 Identity = 40/75 (53.33%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P + KP V DTRKRKGL +P LD Sbjct: 762 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALD 834
BLAST of Elongation factor 2 vs. Ensembl Fly
Match: eEF2 (gene:FBgn0000559 transcript:FBtr0085911) HSP 1 Score: 81.2629 bits (199), Expect = 6.247e-19 Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P +P+ KP V+DTRKRKGL +P L + Sbjct: 766 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQ 839
BLAST of Elongation factor 2 vs. Ensembl Fly
Match: eEF2 (gene:FBgn0000559 transcript:FBtr0085912) HSP 1 Score: 81.2629 bits (199), Expect = 6.247e-19 Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P +P+ KP V+DTRKRKGL +P L + Sbjct: 766 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQ 839
BLAST of Elongation factor 2 vs. Ensembl Fly
Match: eEF2 (gene:FBgn0000559 transcript:FBtr0332966) HSP 1 Score: 81.2629 bits (199), Expect = 6.247e-19 Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P +P+ KP V+DTRKRKGL +P L + Sbjct: 766 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQ 839
BLAST of Elongation factor 2 vs. Ensembl Zebrafish
Match: eef2b (eukaryotic translation elongation factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5128]) HSP 1 Score: 81.2629 bits (199), Expect = 8.634e-19 Identity = 42/75 (56.00%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P+D KP V DTRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPKDAASKPCQIVADTRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. Ensembl Zebrafish
Match: eef2l2 (eukaryotic translation elongation factor 2, like 2 [Source:ZFIN;Acc:ZDB-GENE-030131-8112]) HSP 1 Score: 79.7221 bits (195), Expect = 2.564e-18 Identity = 42/75 (56.00%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D KP V DTRKRKGL IP LD Sbjct: 783 PIFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPYDVNSKPSQIVADTRKRKGLKEGIPALD 855
BLAST of Elongation factor 2 vs. Ensembl Zebrafish
Match: eef2b (eukaryotic translation elongation factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5128]) HSP 1 Score: 79.7221 bits (195), Expect = 2.589e-18 Identity = 42/75 (56.00%), Postives = 47/75 (62.67%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D KP V DTRKRKGL IP LD Sbjct: 782 PIFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPYDVNSKPSQIVADTRKRKGLKEGIPALD 854
BLAST of Elongation factor 2 vs. Ensembl Zebrafish
Match: eef2a.1 (eukaryotic translation elongation factor 2a, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-050522-500]) HSP 1 Score: 65.855 bits (159), Expect = 3.949e-14 Identity = 37/74 (50.00%), Postives = 42/74 (56.76%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPL 284 P L K YL V + F +L A T GQ F QCVFDHWQ+ PG+P DPT K + D RK KGL IP L Sbjct: 778 PVYLLKAYLPV--SESFGFTADLCANTSGQAFSQCVFDHWQILPGDPMDPTSKVAHIMADIRKSKGLDEAIPAL 849 HSP 2 Score: 28.4906 bits (62), Expect = 3.949e-14 Identity = 11/16 (68.75%), Postives = 12/16 (75.00%), Query Frame = 2 Query: 17 KRRGVFFRERQVAGTP 64 +RRGV F E QV GTP Sbjct: 763 RRRGVVFSESQVMGTP 778
BLAST of Elongation factor 2 vs. Ensembl Zebrafish
Match: eef2a.1 (eukaryotic translation elongation factor 2a, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-050522-500]) HSP 1 Score: 65.0846 bits (157), Expect = 4.555e-14 Identity = 31/56 (55.36%), Postives = 35/56 (62.50%), Query Frame = 3 Query: 117 FP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPL 284 F +L A T GQ F QCVFDHWQ+ PG+P DPT K + D RK KGL IP L Sbjct: 100 FTADLCANTSGQAFSQCVFDHWQILPGDPMDPTSKVAHIMADIRKSKGLDEAIPAL 155
BLAST of Elongation factor 2 vs. Ensembl Xenopus
Match: eef2.2 (eukaryotic translation elongation factor 2, gene 2 [Source:NCBI gene;Acc:548502]) HSP 1 Score: 82.8037 bits (203), Expect = 3.344e-19 Identity = 42/75 (56.00%), Postives = 49/75 (65.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IPPLD Sbjct: 768 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSTSRPSQVVGETRKRKGLKEGIPPLD 840
BLAST of Elongation factor 2 vs. Ensembl Xenopus
Match: eef2.2 (eukaryotic translation elongation factor 2, gene 2 [Source:NCBI gene;Acc:548502]) HSP 1 Score: 82.4185 bits (202), Expect = 3.643e-19 Identity = 42/75 (56.00%), Postives = 49/75 (65.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IPPLD Sbjct: 747 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSTSRPSQVVGETRKRKGLKEGIPPLD 819
BLAST of Elongation factor 2 vs. Ensembl Xenopus
Match: eef2.2 (eukaryotic translation elongation factor 2, gene 2 [Source:NCBI gene;Acc:548502]) HSP 1 Score: 82.4185 bits (202), Expect = 3.654e-19 Identity = 42/75 (56.00%), Postives = 49/75 (65.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IPPLD Sbjct: 784 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSTSRPSQVVGETRKRKGLKEGIPPLD 856
BLAST of Elongation factor 2 vs. Ensembl Xenopus
Match: eef2.2 (eukaryotic translation elongation factor 2, gene 2 [Source:NCBI gene;Acc:548502]) HSP 1 Score: 82.4185 bits (202), Expect = 3.950e-19 Identity = 42/75 (56.00%), Postives = 49/75 (65.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IPPLD Sbjct: 787 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSTSRPSQVVGETRKRKGLKEGIPPLD 859
BLAST of Elongation factor 2 vs. Ensembl Xenopus
Match: eef2.2 (eukaryotic translation elongation factor 2, gene 2 [Source:NCBI gene;Acc:548502]) HSP 1 Score: 82.4185 bits (202), Expect = 4.552e-19 Identity = 42/75 (56.00%), Postives = 49/75 (65.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IPPLD Sbjct: 740 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSTSRPSQVVGETRKRKGLKEGIPPLD 812
BLAST of Elongation factor 2 vs. Ensembl Mouse
Match: Eef2 (eukaryotic translation elongation factor 2 [Source:MGI Symbol;Acc:MGI:95288]) HSP 1 Score: 78.1814 bits (191), Expect = 9.139e-18 Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D + +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. UniProt/SwissProt
Match: sp|P13060|EF2_DROME (Elongation factor 2 OS=Drosophila melanogaster OX=7227 GN=EF2 PE=1 SV=4) HSP 1 Score: 81.2629 bits (199), Expect = 6.262e-18 Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P +P+ KP V+DTRKRKGL +P L + Sbjct: 766 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQ 839
BLAST of Elongation factor 2 vs. UniProt/SwissProt
Match: sp|Q3SYU2|EF2_BOVIN (Elongation factor 2 OS=Bos taurus OX=9913 GN=EEF2 PE=2 SV=3) HSP 1 Score: 79.337 bits (194), Expect = 3.055e-17 Identity = 41/75 (54.67%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. UniProt/SwissProt
Match: sp|A0SXL6|EF2_CALJA (Elongation factor 2 OS=Callithrix jacchus OX=9483 GN=EEF2 PE=2 SV=1) HSP 1 Score: 79.337 bits (194), Expect = 3.115e-17 Identity = 41/75 (54.67%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D T +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. UniProt/SwissProt
Match: sp|P58252|EF2_MOUSE (Elongation factor 2 OS=Mus musculus OX=10090 GN=Eef2 PE=1 SV=2) HSP 1 Score: 78.1814 bits (191), Expect = 6.392e-17 Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D + +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. UniProt/SwissProt
Match: sp|P05197|EF2_RAT (Elongation factor 2 OS=Rattus norvegicus OX=10116 GN=Eef2 PE=1 SV=4) HSP 1 Score: 78.1814 bits (191), Expect = 6.455e-17 Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P D + +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. TrEMBL
Match: A0A183VZI7 (Tr-type G domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS5893 PE=4 SV=1) HSP 1 Score: 94.7449 bits (234), Expect = 3.674e-20 Identity = 47/90 (52.22%), Postives = 58/90 (64.44%), Query Frame = 3 Query: 18 KEEACFSGKDKSPELPRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 ++ A +++ + P + K YL V N F +LRA TGGQ FPQCVFDHWQ YPG+P DPT KPG V +TRKRKGL G +P LD Sbjct: 757 RKRAITISEERKSDSPMCIIKAYLPV--NESFGFTTDLRAATGGQAFPQCVFDHWQTYPGDPLDPTSKPGQVVLETRKRKGLNGGVPALD 844
BLAST of Elongation factor 2 vs. TrEMBL
Match: Q804A1 (Elongation factor-2 (Fragment) OS=Pseudopleuronectes americanus OX=8265 GN=EF2 PE=2 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 4.120e-19 Identity = 38/58 (65.52%), Postives = 44/58 (75.86%), Query Frame = 3 Query: 117 FP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 F +LR+ TGGQ FPQCVFDHWQ+ PGNP DPT KPGI V DTRKRKG+ +P L+ Sbjct: 22 FTADLRSSTGGQAFPQCVFDHWQILPGNPMDPTSKPGIVVTDTRKRKGIKEGVPALEN 79
BLAST of Elongation factor 2 vs. TrEMBL
Match: A0A0B7AWJ0 (Tr-type G domain-containing protein OS=Arion vulgaris OX=1028688 GN=ORF142525 PE=4 SV=1) HSP 1 Score: 90.8929 bits (224), Expect = 7.528e-19 Identity = 40/66 (60.61%), Postives = 49/66 (74.24%), Query Frame = 3 Query: 90 HV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 H+ N F +LR+ TGGQ FPQCVFDHWQ+ PG+P+DPT KPG+ V ++RKRKGL IPPLD Sbjct: 771 HMPVNESFGFTADLRSNTGGQAFPQCVFDHWQIMPGDPKDPTTKPGVIVIESRKRKGLKEGIPPLD 836
BLAST of Elongation factor 2 vs. TrEMBL
Match: A0A1X7U235 (Tr-type G domain-containing protein OS=Amphimedon queenslandica OX=400682 GN=100641942 PE=4 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 9.723e-19 Identity = 44/76 (57.89%), Postives = 51/76 (67.11%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 P + + YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P DPT KPGI V +TRKRK L IP LDK Sbjct: 694 PMFMVRAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPHDPTTKPGIVVTETRKRKALSEGIPGLDK 767
BLAST of Elongation factor 2 vs. TrEMBL
Match: Q6AZM9 (Eft-2-prov protein OS=Xenopus laevis OX=8355 GN=eef2.2 PE=2 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 1.058e-18 Identity = 46/91 (50.55%), Postives = 55/91 (60.44%), Query Frame = 3 Query: 18 KEEACFSGKDKSPELPRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 K+ +++ +P K +L V N F +LRA TGGQ FPQCVFDHWQ YPGNP DP+ KPG V RKRKGL +IP LDK Sbjct: 757 KKRGVIQSEERVAGMPVCCVKAFLPV--NESFGFTTDLRANTGGQAFPQCVFDHWQQYPGNPLDPSSKPGQAVLAIRKRKGLSDEIPSLDK 845
BLAST of Elongation factor 2 vs. Ensembl Cavefish
Match: eef2b (eukaryotic translation elongation factor 2 [Source:NCBI gene;Acc:103031761]) HSP 1 Score: 79.337 bits (194), Expect = 3.338e-18 Identity = 41/75 (54.67%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ G+P+D T KP V + RKRKGL IPPLD Sbjct: 805 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILQGDPKDATSKPCQIVAEIRKRKGLKEGIPPLD 877
BLAST of Elongation factor 2 vs. Ensembl Cavefish
Match: eef2b (eukaryotic translation elongation factor 2 [Source:NCBI gene;Acc:103031761]) HSP 1 Score: 78.9518 bits (193), Expect = 4.247e-18 Identity = 41/75 (54.67%), Postives = 48/75 (64.00%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ G+P+D T KP V + RKRKGL IPPLD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILQGDPKDATSKPCQIVAEIRKRKGLKEGIPPLD 852
BLAST of Elongation factor 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001336.1 (pep scaffold:Pmarinus_7.0:GL476352:246829:320669:1 gene:ENSPMAG00000001142.1 transcript:ENSPMAT00000001336.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 84.7297 bits (208), Expect = 8.289e-21 Identity = 42/75 (56.00%), Postives = 49/75 (65.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ PG+P DPT +P V +TRKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPSDPTTRPAQVVVETRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. Ensembl Yeast
Match: EFT2 (Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication [Source:SGD;Acc:S000002793]) HSP 1 Score: 62.3882 bits (150), Expect = 3.162e-13 Identity = 35/79 (44.30%), Postives = 42/79 (53.16%), Query Frame = 3 Query: 42 KDKSPELPRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIP 278 +++ P P K YL V N F ELR TGGQ FPQ VFDHW +P DPT K G V RKR G+ ++P Sbjct: 757 EEQRPGTPLFTVKAYLPV--NESFGFTGELRQATGGQAFPQMVFDHWSTLGSDPLDPTSKAGEIVLAARKRHGMKEEVP 833
BLAST of Elongation factor 2 vs. Ensembl Yeast
Match: EFT1 (Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication [Source:SGD;Acc:S000005659]) HSP 1 Score: 62.3882 bits (150), Expect = 3.162e-13 Identity = 35/79 (44.30%), Postives = 42/79 (53.16%), Query Frame = 3 Query: 42 KDKSPELPRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIP 278 +++ P P K YL V N F ELR TGGQ FPQ VFDHW +P DPT K G V RKR G+ ++P Sbjct: 757 EEQRPGTPLFTVKAYLPV--NESFGFTGELRQATGGQAFPQMVFDHWSTLGSDPLDPTSKAGEIVLAARKRHGMKEEVP 833
BLAST of Elongation factor 2 vs. Ensembl Nematostella
Match: EDO39908 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8P5]) HSP 1 Score: 70.0922 bits (170), Expect = 1.207e-17 Identity = 33/54 (61.11%), Postives = 39/54 (72.22%), Query Frame = 3 Query: 126 ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 +LR++TGGQ FPQCVFDHWQ+ PG+ D PG V +TRKRKGL IP LD Sbjct: 1 DLRSKTGGQAFPQCVFDHWQVLPGDVHDLASMPGQVVANTRKRKGLKEGIPALD 54
BLAST of Elongation factor 2 vs. Ensembl Nematostella
Match: EDO45605 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB9]) HSP 1 Score: 73.1738 bits (178), Expect = 1.474e-16 Identity = 34/57 (59.65%), Postives = 40/57 (70.18%), Query Frame = 3 Query: 117 FP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 F +LR++TGGQ FPQCVFDHWQ+ PG+ D PG V +TRKRKGL IP LD Sbjct: 769 FTADLRSKTGGQAFPQCVFDHWQVLPGDVHDLASMPGQVVANTRKRKGLKEGIPALD 825
BLAST of Elongation factor 2 vs. Ensembl Medaka
Match: ENSORLT00000005814.2 (elongation factor 2 [Source:NCBI gene;Acc:101171982]) HSP 1 Score: 87.8113 bits (216), Expect = 3.035e-21 Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = 3 Query: 117 FP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 F +LR+ TGGQ FPQC+FDHWQ+ PGNP +PT KPG+ V DTRKRKGL +P LD Sbjct: 796 FTADLRSNTGGQAFPQCMFDHWQILPGNPMEPTSKPGVVVTDTRKRKGLKEGVPALD 852
BLAST of Elongation factor 2 vs. Ensembl Medaka
Match: EEF2 (eukaryotic translation elongation factor 2 [Source:NCBI gene;Acc:101162630]) HSP 1 Score: 76.2554 bits (186), Expect = 3.984e-17 Identity = 39/75 (52.00%), Postives = 46/75 (61.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ G+P DP +P V + RKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILQGDPNDPATRPCQVVAEIRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. Ensembl Medaka
Match: EEF2 (eukaryotic translation elongation factor 2 [Source:NCBI gene;Acc:101162630]) HSP 1 Score: 75.8702 bits (185), Expect = 4.474e-17 Identity = 39/75 (52.00%), Postives = 46/75 (61.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ G+P DP +P V + RKRKGL IP LD Sbjct: 780 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILQGDPNDPATRPCQVVAEIRKRKGLKEGIPALD 852
BLAST of Elongation factor 2 vs. Ensembl Medaka
Match: EEF2 (eukaryotic translation elongation factor 2 [Source:NCBI gene;Acc:101162630]) HSP 1 Score: 75.8702 bits (185), Expect = 5.022e-17 Identity = 39/75 (52.00%), Postives = 46/75 (61.33%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLD 287 P + K YL V N F +LR+ TGGQ FPQCVFDHWQ+ G+P DP +P V + RKRKGL IP LD Sbjct: 768 PMFVVKAYLPV--NESFGFTADLRSNTGGQAFPQCVFDHWQILQGDPNDPATRPCQVVAEIRKRKGLKEGIPALD 840
BLAST of Elongation factor 2 vs. Planmine SMEST
Match: SMESG000042430.1 (SMESG000042430.1) HSP 1 Score: 116.701 bits (291), Expect = 1.962e-31 Identity = 56/76 (73.68%), Postives = 59/76 (77.63%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGLFGDIPPLDK 290 P+ + K YL V N +F ELRAQTGGQ FPQCVFDHWQLYPGNP DPT KPGI VRDTRKRKGL DIPPLDK Sbjct: 771 PQYVVKAYLPV--NESFNFTSELRAQTGGQAFPQCVFDHWQLYPGNPLDPTTKPGITVRDTRKRKGLSEDIPPLDK 844 HSP 2 Score: 46.9802 bits (110), Expect = 4.511e-7 Identity = 25/67 (37.31%), Postives = 34/67 (50.75%), Query Frame = 2 Query: 2 HSSINKRRGVFFRERQVAGTPQDAVKEIFTRVTQCNCTFSIRVESSNRWAGFPSMCF*SLAIISGKP 202 +S++NKRRG+ F E QVAGTPQ VK N T +R ++ + FP F + G P Sbjct: 751 YSTLNKRRGIIFSEMQVAGTPQYVVKAYLPVNESFNFTSELRAQTGGQ--AFPQCVFDHWQLYPGNP 815
BLAST of Elongation factor 2 vs. Planmine SMEST
Match: SMESG000076126.1 (SMESG000076126.1) HSP 1 Score: 94.7449 bits (234), Expect = 1.010e-23 Identity = 46/77 (59.74%), Postives = 56/77 (72.73%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGL-FGDIPPLDK 290 P+ + K +L V N +F +LRAQTGGQ FPQCVFDHWQ++PGNP DPT KPG+ V RKRKGL G +PPLD+ Sbjct: 750 PQYIVKAHLPV--NESFNFTSDLRAQTGGQAFPQCVFDHWQMFPGNPLDPTSKPGLVVMAARKRKGLPDGGVPPLDR 824
BLAST of Elongation factor 2 vs. Planmine SMEST
Match: SMESG000076108.1 (SMESG000076108.1) HSP 1 Score: 94.7449 bits (234), Expect = 1.010e-23 Identity = 46/77 (59.74%), Postives = 56/77 (72.73%), Query Frame = 3 Query: 63 PRML*KRYLHV*PNAIVHFP*ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMKPGIQVRDTRKRKGL-FGDIPPLDK 290 P+ + K +L V N +F +LRAQTGGQ FPQCVFDHWQ++PGNP DPT KPG+ V RKRKGL G +PPLD+ Sbjct: 750 PQYIVKAHLPV--NESFNFTSDLRAQTGGQAFPQCVFDHWQMFPGNPLDPTSKPGLVVMAARKRKGLPDGGVPPLDR 824
BLAST of Elongation factor 2 vs. Planmine SMEST
Match: SMESG000045982.1 (SMESG000045982.1) HSP 1 Score: 43.5134 bits (101), Expect = 6.509e-6 Identity = 26/62 (41.94%), Postives = 33/62 (53.23%), Query Frame = 3 Query: 126 ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMK--------PGIQVRD----TRKRKGLFGDI 275 +LR T GQ F Q VF HWQ PG+P D ++K P R+ TR+RKGL D+ Sbjct: 312 DLRTFTQGQSFCQSVFHHWQQVPGDPLDRSIKLKPLIAQPPTHLAREFMVKTRRRKGLNEDV 373
BLAST of Elongation factor 2 vs. Planmine SMEST
Match: SMESG000045982.1 (SMESG000045982.1) HSP 1 Score: 41.9726 bits (97), Expect = 6.868e-6 Identity = 26/62 (41.94%), Postives = 33/62 (53.23%), Query Frame = 3 Query: 126 ELRAQTGGQDFPQCVFDHWQLYPGNPQDPTMK--------PGIQVRD----TRKRKGLFGDI 275 +LR T GQ F Q VF HWQ PG+P D ++K P R+ TR+RKGL D+ Sbjct: 16 DLRTFTQGQSFCQSVFHHWQQVPGDPLDRSIKLKPLIAQPPTHLAREFMVKTRRRKGLNEDV 77 The following BLAST results are available for this feature:
BLAST of Elongation factor 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of Elongation factor 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Elongation factor 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of Elongation factor 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Elongation factor 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Elongation factor 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of Elongation factor 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Elongation factor 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Elongation factor 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of Elongation factor 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Elongation factor 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of Elongation factor 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of Elongation factor 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 4
BLAST of Elongation factor 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030239 ID=SMED30030239|Name=Elongation factor 2|organism=Schmidtea mediterranea sexual|type=transcript|length=340bpback to top protein sequence of SMED30030239-orf-1 >SMED30030239-orf-1 ID=SMED30030239-orf-1|Name=SMED30030239-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp DPNIYLIDWNLKFTPTFYLRVEYPQKVLFFFSCLELGFLVSLWDPVGFPDback to top Annotated Terms
The following terms have been associated with this transcript:
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