TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein

Overview
NameTATA box-binding protein-associated factor RNA polymerase I subunit A-like protein
Smed IDSMED30030223
Length (bp)1277
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein (SMED30030223) t-SNE clustered cells

Violin plots show distribution of expression levels for TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein (SMED30030223) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein (SMED30030223) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein (SMED30030223) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030223

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30030223SMESG000023715.1 SmedASXL_009923SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. TrEMBL
Match: A0A5K3EIG7 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.561e-14
Identity = 92/361 (25.48%), Postives = 157/361 (43.49%), Query Frame = 2 
Query:  194 SISNNREACIRLIYTKLSRNDIVDALAVFTILLLNFKISTSFLWRTGFHILSRIGDNSSMKIFFNTLLEYKVWDPSNMIXXXXXXXXXXXXXDEIKHIYRNQYSV-YFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQ----AELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCS-----LFLLQAFKVHI------ENLND---------------EGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSP 1183
            S+  +R+  + +++  LSR+   +A  +    + N+ +S S++WR  FHI       + + IF   +  +++ D +N I E +L  +  K ++       ++ S    R     RE E   +    RL   +SY+A W   LN   +D  S  Q    A+  F E+ S ID   + D FI  Y+ +L ++ + SEAL +L++Y  +  NN +T  YL ++       +  S   E+  E R + +E+ + ++ +C      L  L AF + +       NL D                G HE  + + FK L    W +   PW  L   I  +G SS+ +  LW      W   + S P
Sbjct:   56 SLYCDRQRIMCMMWILLSRHMFAEAAKLMVFAIHNYSMSPSWIWRAAFHIFRCARRPAGLCIFSRNMNPFQMIDENNRILEAILSQMVIKNWEGAVAERPDKLSQKRMRNVDLSREPECLHVQRLQRLYEGLSYFALWH--LN---KDRPSGAQYLEKAKNRFSEVESLIDHGHICDVFIRPYVHILLHEDQASEALELLQKYATQQTNNPNTDYYLAEWYLHTA--IGRSAFPENADEFR-EVLEEALPVSVDCEEMPPLLKHLVAFSLRLLPMPAAPNLVDADKSRHCIIADICCSRGFHEAALDIAFKLLDHPSWCRFRRPWLLLQTCISKIGISSEQVSRLWTLRTATWKLYVLSRP 408
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. TrEMBL
Match: A0A158QU28 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS5685 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.688e-12
Identity = 87/339 (25.66%), Postives = 144/339 (42.48%), Query Frame = 2 
Query:  260 VDALAVFTILLLNFKISTSFLWRTGFHILSRIGDNSSMKIFFNTLLEYKVWDPSNMIXXXXXXXXXXXXXDEIKHIYRNQYSV-YFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQ----AELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCS-----LFLLQAFKVHI------ENLND---------------EGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSP 1183
             +A  +    + N+ +S S++WR  FHI       + + IF   +  +++ D +N I E +L  +  K ++       ++ S    R     RE E   +    RL   +SY+A W   LN   +D  S  Q    A+  F E+ S ID   + D FI  Y+ +L ++ + SEAL +L++Y  +  NN +T  YL ++       +  S   E+  E R + +E+ + ++ +C      L  L AF + +       NL D                G HE  + + FK L    W +   PW  L   I  +G SS+ +  LW      W   + S P
Sbjct:    3 AEAAKLMVFAIHNYSMSPSWIWRAAFHIFRCARRPAGLCIFSRNMNPFQMIDENNRILEAILSQMVIKNWEGAVAERPDKLSQKRMRNVDLSREPECLHVQRLQRLYEGLSYFALWH--LN---KDRPSGAQYLEKAKNRFSEVESLIDHGHICDVFIRPYVHILLHEDQASEALELLQKYATQQTNNPNTDYYLAEWYLHTA--IGRSAFPENADEFR-EVLEEALPVSVDCEEMPPLLKHLVAFSLRLLPMPAAPNLVDADKSRHCIIADICCSRGFHEAALDIAFKLLDHPSWCRFRRPWLLLQTCISKIGISSEQVSRLWTLRTATWKLYVLSRP 333
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. TrEMBL
Match: A0A5K3EK43 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 9.982e-11
Identity = 87/339 (25.66%), Postives = 149/339 (43.95%), Query Frame = 2 
Query:  194 SISNNREACIRLIYTKLSRNDIVDALAVFTILLLNFKISTSFLWRTGFHILSRIGDNSSMKIFFNTLLEYKVWDPSNMIXXXXXXXXXXXXXDEIKHIYRNQYSV-YFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQ----AELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCS-----LFLLQAFKVHI------ENLND---------------EGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASS 1117
            S+  +R+  + +++  LSR+   +A  +    + N+ +S S++WR  FHI       + + IF   +  +++ D +N I E +L  +  K ++       ++ S    R     RE E   +    RL   +SY+A W   LN   +D  S  Q    A+  F E+ S ID   + D FI  Y+ +L ++ + SEAL +L++Y  +  NN +T  YL ++       +  S   E+  E R + +E+ + ++ +C      L  L AF + +       NL D                G HE  + + FK L    W +   PW  L   I  +G SS
Sbjct:   56 SLYCDRQRIMCMMWILLSRHMFAEAAKLMVFAIHNYSMSPSWIWRAAFHIFRCARRPAGLCIFSRNMNPFQMIDENNRILEAILSQMVIKNWEGAVAERPDKLSQKRMRNVDLSREPECLHVQRLQRLYEGLSYFALWH--LN---KDRPSGAQYLEKAKNRFSEVESLIDHGHICDVFIRPYVHILLHEDQASEALELLQKYATQQTNNPNTDYYLAEWYLHTA--IGRSAFPENADEFR-EVLEEALPVSVDCEEMPPLLKHLVAFSLRLLPMPAAPNLVDADKSRHCIIADICCSRGFHEAALDIAFKLLDHPSWCRFRRPWLLLQTCISKIGISS 386
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. TrEMBL
Match: A0A087VWY7 (Expressed conserved protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000055600 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.698e-8
Identity = 82/374 (21.93%), Postives = 159/374 (42.51%), Query Frame = 2 
Query:  158 IKLIGEPLDHQVSISNNREACIRLIYTKLSRNDIVDALAVFTILLLNFKISTSFLWRTGFHILSRIGDNSSMKIFFNTLLEYKVWDPSNM-IXXXXXXXXXXXXXDEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATW--KQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHL----QESVTNEDIVEIR---TQYIEKH-IQINPNCSLFLLQAFKVHI-------ENLNDEGN--------------HEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSP 1183
            ++ +  P     ++S +R+  + +++  LSR+   +A  +    + N+ +S S++WR  + I + +  + S + F   L ++ + D +N  +   L  ++       I     N  S   ++    RE E   +    RL   ++Y+A W   +    GS+  E   +AE    E+ + I+   + D F+L  + +   +    + L +L++Y    P+N +T  +L       ++HL      +V   D  E R   T+ +  H + I P   L  L AF + +       +++  +GN                + + + FK L    W + + PWR L   ++ LGA+SD +  +W      W   IFS P
Sbjct:   35 VQCVNSPWQTFETLSCDRQRIMCMMWVLLSRHKFTEAAELMVYAIHNYTLSPSWIWRATYQIFNCMKVSISSEKFNKVLNQFHMTDENNRNLETLLSQMVGGNWKAAIDSRLDNNPSRRVQSTDRCREPESLHVQRLHRLYEGLAYFALWFLNRERPAGSKYLE---KAEYRLSEVETLIEFGHIGDVFLLPLVQIHFDKGCAEDILPLLEKYASLQPHNPNTDRFL------AEWHLYAAIGHTVLPRDPNEFRRIVTKSLTLHGVSIPP--LLKHLVAFSLRLLPTPASSDSVRGDGNWYFIIADICRASGFFTQALEIAFKLLDHPSWCKFDQPWRLLQTCVQKLGATSDFVSKMWTPRAATWKHYIFSHP 397
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. TrEMBL
Match: A0A4E0RHV2 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_002022 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 7.147e-7
Identity = 64/265 (24.15%), Postives = 118/265 (44.53%), Query Frame = 2 
Query:  176 PLDHQVSISNNREACIRLIYTKLSRNDIVDALAVFTILLLNFKISTSFLWRTGFHILSRIGDNSSMKIFFNTLLEYKVWDPSNMIXXXXXXXXXXXXXDEI------KHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATW-KQSLNLGSED--EESRIQAELE---------FKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVT----NEDIVEIRT 904
            P D ++ + + +   +  +++ LS+ +  +A  +    L  F ++ S++WR  FHI   +    S + F   L ++++ D +N + E +L+ + Q   D        K  +R++Y+         RE E E +    RL   +S Y  W +QS+ +   D  +  R QA  +          +E+ + I    + D FI   ++ LEY  + + A R+L RY    P N +T  YL ++  R     Q        NE +V+ +T
Sbjct:   48 PEDFRLCLRSEKLRIMCFMWSLLSKYEFKEAARLLIHSLHTFPMAASWIWRVAFHIFRCLNAPESFRKFNKVLDQFRLADENNRVLERILHHMVQGDLDAAHKCRSHKVTHRDRYTSV--ESIITREPEFERVQRLQRLYEGLSLYCLWNEQSVGISVSDDHDHDRDQAAADELAEKAFHRLQEVEAMISGGYLCDVFIRPLVETLEYFGESARARRLLVRYKRHVPENPNTLRYLCEWYKRQHVTDQSEPMNLSDNEPVVDAKT 310
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Cavefish
Match: taf1a (TATA-box binding protein associated factor, RNA polymerase I subunit A [Source:NCBI gene;Acc:103041075])

HSP 1 Score: 50.447 bits (119), Expect = 2.094e-6
Identity = 45/174 (25.86%), Postives = 80/174 (45.98%), Query Frame = 2 
Query:  650 QAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKR--YHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLL--ISIRHLGASSDIIQNLWIFIKEFW 1159
            QA +  +E+   I +  V DPF+L+Y+D+LE+     EAL+VL+   Y++  P+N +   YL  FL R++    + ++   I+++    +  H  +   C L +      H+         EE + ++F  L    W      W  LL  +++        +I N W   K+FW
Sbjct:  218 QASVTLQEI---IAQPGVWDPFVLSYVDMLEFYNDQEEALKVLQNYAYNKDYPSNPNAHVYLYQFLKRHEAPPAKLIS---ILKVLHHLVPSHELMQEYCYLLVNSVVPGHL---------EESLPVVFNLLDYPSWKNDLDTWNCLLTVMTVFKRKGLQHLIANEWKQRKDFW 376
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Cavefish
Match: taf1a (TATA-box binding protein associated factor, RNA polymerase I subunit A [Source:NCBI gene;Acc:103041075])

HSP 1 Score: 50.447 bits (119), Expect = 2.209e-6
Identity = 45/174 (25.86%), Postives = 80/174 (45.98%), Query Frame = 2 
Query:  650 QAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKR--YHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLL--ISIRHLGASSDIIQNLWIFIKEFW 1159
            QA +  +E+   I +  V DPF+L+Y+D+LE+     EAL+VL+   Y++  P+N +   YL  FL R++    + ++   I+++    +  H  +   C L +      H+         EE + ++F  L    W      W  LL  +++        +I N W   K+FW
Sbjct:  218 QASVTLQEI---IAQPGVWDPFVLSYVDMLEFYNDQEEALKVLQNYAYNKDYPSNPNAHVYLYQFLKRHEAPPAKLIS---ILKVLHHLVPSHELMQEYCYLLVNSVVPGHL---------EESLPVVFNLLDYPSWKNDLDTWNCLLTVMTVFKRKGLQHLIANEWKQRKDFW 376
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Cavefish
Match: taf1a (TATA-box binding protein associated factor, RNA polymerase I subunit A [Source:NCBI gene;Acc:103041075])

HSP 1 Score: 50.447 bits (119), Expect = 2.312e-6
Identity = 45/174 (25.86%), Postives = 80/174 (45.98%), Query Frame = 2 
Query:  650 QAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKR--YHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLL--ISIRHLGASSDIIQNLWIFIKEFW 1159
            QA +  +E+   I +  V DPF+L+Y+D+LE+     EAL+VL+   Y++  P+N +   YL  FL R++    + ++   I+++    +  H  +   C L +      H+         EE + ++F  L    W      W  LL  +++        +I N W   K+FW
Sbjct:  218 QASVTLQEI---IAQPGVWDPFVLSYVDMLEFYNDQEEALKVLQNYAYNKDYPSNPNAHVYLYQFLKRHEAPPAKLIS---ILKVLHHLVPSHELMQEYCYLLVNSVVPGHL---------EESLPVVFNLLDYPSWKNDLDTWNCLLTVMTVFKRKGLQHLIANEWKQRKDFW 376
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Planmine SMEST
Match: SMESG000023715.1 (SMESG000023715.1)

HSP 1 Score: 549.666 bits (1415), Expect = 0.000e+0
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 2 
Query:  371 MKIFFNTLLEYKVWDPSNMIXXXXXXXXXXXXXDEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIKELMVMLA 1222
            MKIFFNTLLEYKVWDPSNMIYEYLLYLLEQKKYDEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIKELMVMLA
Sbjct:    1 MKIFFNTLLEYKVWDPSNMIYEYLLYLLEQKKYDEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIKELMVMLA 284
[Close]
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Planmine SMEST
Match: SMESG000023715.1 (SMESG000023715.1)

HSP 1 Score: 512.686 bits (1319), Expect = 0.000e+0
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 2 
Query:  470 DEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIKELMVMLA 1222
            DEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIKELMVMLA
Sbjct:   16 DEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYATWKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQKFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIRTQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVKWGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIKELMVMLA 266
The following BLAST results are available for this feature:
BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K3EIG74.561e-1425.48Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A158QU283.688e-1225.66Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A5K3EK439.982e-1125.66Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A087VWY71.698e-821.93Expressed conserved protein OS=Echinococcus multil... [more]
A0A4E0RHV27.147e-724.15Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
taf1a2.094e-625.86TATA-box binding protein associated factor, RNA po... [more]
taf1a2.209e-625.86TATA-box binding protein associated factor, RNA po... [more]
taf1a2.312e-625.86TATA-box binding protein associated factor, RNA po... [more]
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000023715.10.000e+0100.00SMESG000023715.1[more]
SMESG000023715.10.000e+0100.00SMESG000023715.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030223 ID=SMED30030223|Name=TATA box-binding protein-associated factor RNA polymerase I subunit A-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1277bp
AATGAATCGAAATAAGTTTTTCAAATCATATAAAAATAATGGAAAATCAA
TGGATAGTGAGGCATCATTTGAACATCTAGCAAGTATCTTTCATTCTATT
TTGAAAAAAAGAACTAAATTAAAACATAAATCCTTTACTTTATTTGATGA
GAATTTAATCAAGCTTATTGGGGAACCTTTAGATCATCAAGTTAGCATTA
GCAACAATAGAGAAGCATGTATTCGATTAATCTATACTAAACTTTCGCGA
AATGATATTGTTGATGCTCTAGCTGTTTTCACCATTTTATTACTTAACTT
CAAAATCAGTACCTCTTTTCTTTGGAGAACTGGATTTCATATTTTATCAC
GCATTGGAGATAACAGTTCCATGAAAATTTTCTTTAACACATTATTAGAA
TATAAAGTTTGGGATCCTTCAAATATGATCTATGAATATTTACTCTATTT
ATTAGAACAGAAAAAGTATGATGAAATTAAACATATTTATAGGAACCAGT
ATTCAGTATATTTCCGGACTAAGAGATTTATCAGGGAATCGGAAATGGAA
ATTTTGAATCACTATATCAGGTTGATTGTCGCGGTTTCATACTACGCCAC
TTGGAAGCAGTCATTAAATTTGGGTTCCGAAGATGAAGAATCTCGAATCC
AGGCCGAATTAGAATTCAAAGAATTATTTTCTGCCATTGATAAAAAATTA
GTCCTCGACCCCTTTATTTTGAATTATCTAGACTTGTTGGAATATCAGCA
GAAATTTTCGGAGGCCCTTCGTGTTCTTAAACGGTATCATGAAAAATGTC
CCAATAATATTCATACTTCAGAATATCTCTTGGACTTTTTGTCTCGTAAT
CAATATCACTTACAAGAATCGGTAACGAATGAAGACATCGTAGAGATTCG
AACGCAATACATTGAGAAACATATTCAGATTAATCCCAATTGCTCTTTGT
TCCTTTTGCAGGCATTTAAAGTGCATATTGAAAACCTCAATGATGAGGGA
AATCACGAGGAAATCGTTAGATTATTGTTTAAATATCTCAGTGATGTCAA
GTGGGGACAAGTCGAGTGGCCTTGGAGATATCTTTTGATCTCAATTCGGC
ATTTGGGGGCATCCAGTGATATTATTCAAAATTTGTGGATCTTTATTAAA
GAATTTTGGAATGATGAAATCTTCTCAAGTCCGCGCAACAGCTACATAAA
GGAACTGATGGTAATGTTAGCGTAGCCGATGGTATGTGATTTCGTTGTCT
GCCTTAGAATAAAATGTTTCTATTGCC
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protein sequence of SMED30030223-orf-1

>SMED30030223-orf-1 ID=SMED30030223-orf-1|Name=SMED30030223-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=408bp
MNRNKFFKSYKNNGKSMDSEASFEHLASIFHSILKKRTKLKHKSFTLFDE
NLIKLIGEPLDHQVSISNNREACIRLIYTKLSRNDIVDALAVFTILLLNF
KISTSFLWRTGFHILSRIGDNSSMKIFFNTLLEYKVWDPSNMIYEYLLYL
LEQKKYDEIKHIYRNQYSVYFRTKRFIRESEMEILNHYIRLIVAVSYYAT
WKQSLNLGSEDEESRIQAELEFKELFSAIDKKLVLDPFILNYLDLLEYQQ
KFSEALRVLKRYHEKCPNNIHTSEYLLDFLSRNQYHLQESVTNEDIVEIR
TQYIEKHIQINPNCSLFLLQAFKVHIENLNDEGNHEEIVRLLFKYLSDVK
WGQVEWPWRYLLISIRHLGASSDIIQNLWIFIKEFWNDEIFSSPRNSYIK
ELMVMLA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR039495TAF1A-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039495TATA box-binding protein-associated factor RNA polymerase I subunit A-likePFAMPF14929TAF1_subAcoord: 104..393
e-value: 3.3E-13
score: 49.8
NoneNo IPR availableTMHMMTMhelixcoord: 86..108