Helicase for meiosis 1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030220 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 12
Homology
BLAST of Helicase for meiosis 1 vs. Ensembl Human
Match: HFM1 (helicase for meiosis 1 [Source:HGNC Symbol;Acc:HGNC:20193]) HSP 1 Score: 183.726 bits (465), Expect = 1.799e-103 Identity = 103/294 (35.03%), Postives = 163/294 (55.44%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDS--MLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYL 1715
++ I+ C E +I LR E+ +N LNK +RFP++GRI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D+ +++ + L++ +L KCF KLW+N+ VS Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + P+YEL +EQI + + VT + + +Q + R +DS + LIIGD +N V Y +I + L+ + W +I V R + L I+LIS VG DI+ K YL
Sbjct: 810 VTLIAGCKEFLDIQLRINEKKTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTAKIFRHGSRITRWLSDF---VAAQEKKFAVLLNSLILAKCFRCKLWENSLHVSKQLEKIGITLSNAIVNAGLTSFKKIEETDARELELILNRHPPFGTQIKETVMYLPKYELKVEQITRYSD--TTAEILVTVILRNFEQLQTK---RTASDSHYVTLIIGDADNQVVYLHKITDSVLLKAGSWAKKIAVKRALKSEDLSINLISSEFVGLDIQQKLTVFYL 1095 HSP 2 Score: 139.813 bits (351), Expect = 1.799e-103 Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT 414
FC TRK Q A LV +F + +Q +L + + + KLR + G +HHAG+ DR++VE +F G LPVL +TSTL+MGVNLPAHLVVIK T ++ + + Y++ +LQMIGRAGRPQFD +A A+I+T
Sbjct: 533 FCATRKGVQQAASVLVKDAKFIMTVEQKQRLQKYAYSVRDSKLRDILKDGAAYHHAGMELSDRKVVEGAFTVGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYAGGLFEEYSETDILQMIGRAGRPQFDTTATAVIMT 670 HSP 3 Score: 97.8265 bits (242), Expect = 1.799e-103 Identity = 50/121 (41.32%), Postives = 77/121 (63.64%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ +WI+S+ LYIR +NP++YG +G + I + +L + ++ LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL+ LV
Sbjct: 691 SLHRHLIEHLNAEIVLHTITDVNIAVEWIRSTLLYIRALKNPSHYGFASGLNKDGIEAKLQELCLKNLNDLSSLDLIKMDEGVN-FKPTEAGRLMAWYYITFETVKKFYTISGKETLSDLV 810
BLAST of Helicase for meiosis 1 vs. Ensembl Human
Match: HFM1 (helicase for meiosis 1 [Source:HGNC Symbol;Acc:HGNC:20193]) HSP 1 Score: 142.124 bits (357), Expect = 3.220e-38 Identity = 90/294 (30.61%), Postives = 138/294 (46.94%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDS--MLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYL 1715
++ I+ C E +I LR E+ +N LNK +RFP++GRI MK++CL+Q LGC+ + D +L Q+T KIFR G RI RC+ ++ + NAG ++ +I + A+ +E ILN+ PPFG + + P+YEL +EQI + + VT + + +Q + R +DS + LIIGD +N V Y +I + L+ + W +I V R + L I+LIS VG DI+ K YL
Sbjct: 66 VTLIAGCKEFLDIQLRINEKKTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTAKIFRHGSRITRCI-----------------------------------------------TLSNAIVNAGLTSFKKIEETDARELELILNRHPPFGTQIKETVMYLPKYELKVEQITRYSD--TTAEILVTVILRNFEQLQTK---RTASDSHYVTLIIGDADNQVVYLHKITDSVLLKAGSWAKKIAVKRALKSEDLSINLISSEFVGLDIQQKLTVFYL 307 HSP 2 Score: 39.2762 bits (90), Expect = 3.220e-38 Identity = 23/66 (34.85%), Postives = 38/66 (57.58%), Query Frame = 2
Query: 593 IPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
I + +L + ++ LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL+ LV
Sbjct: 2 IEAKLQELCLKNLNDLSSLDLIKMDEGVN-FKPTEAGRLMAWYYITFETVKKFYTISGKETLSDLV 66
BLAST of Helicase for meiosis 1 vs. Ensembl Human
Match: SNRNP200 (small nuclear ribonucleoprotein U5 subunit 200 [Source:HGNC Symbol;Acc:HGNC:30859]) HSP 1 Score: 89.3521 bits (220), Expect = 8.993e-30 Identity = 47/111 (42.34%), Postives = 64/111 (57.66%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D I++TS E
Sbjct: 773 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 875 HSP 2 Score: 48.9062 bits (115), Expect = 8.993e-30 Identity = 28/109 (25.69%), Postives = 53/109 (48.62%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSIC--EAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LYIR+ R+P YG+ + + Q + L+ + L +L+ +D+ T + T G + + Y++ T+Q +L
Sbjct: 896 KLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQL 1004 HSP 3 Score: 35.039 bits (79), Expect = 8.993e-30 Identity = 39/184 (21.20%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +TL C ++ K ++W + + ++PE V ++ N F L + +++ L + P G+T+ K FP+ EL++
Sbjct: 1019 SLSSEFKNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDK----RMWQSMCPLRQFRKLPE-EVVKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHLFPKLELSV 1188 HSP 4 Score: 78.5666 bits (192), Expect = 1.567e-18 Identity = 41/116 (35.34%), Postives = 67/116 (57.76%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEEFAHQF 444
+K+S+ L+ ++ GVG+ H GL ++R +VE F GA+ V+ ++ +L G+N+ AHLV+I T +N I Y +LQM+G A RP Q D I+ S+++F +F
Sbjct: 1602 EKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKF 1717 HSP 5 Score: 36.5798 bits (83), Expect = 1.567e-18 Identity = 24/101 (23.76%), Postives = 50/101 (49.50%), Query Frame = 2
Query: 449 EHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
+H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1734 DHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGISHRHLSDHLSELVEQTLSDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1830
BLAST of Helicase for meiosis 1 vs. Ensembl Human
Match: SNRNP200 (small nuclear ribonucleoprotein U5 subunit 200 [Source:HGNC Symbol;Acc:HGNC:30859]) HSP 1 Score: 85.8853 bits (211), Expect = 1.395e-28 Identity = 47/111 (42.34%), Postives = 64/111 (57.66%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D I++TS E
Sbjct: 773 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 875 HSP 2 Score: 49.2914 bits (116), Expect = 1.395e-28 Identity = 28/108 (25.93%), Postives = 53/108 (49.07%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSIC--EAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LYIR+ R+P YG+ + + Q + L+ + L +L+ +D+ T + T G + + Y++ T+Q +L
Sbjct: 897 LPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQL 1004 HSP 3 Score: 34.2686 bits (77), Expect = 1.395e-28 Identity = 39/184 (21.20%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +TL C ++ K ++W + + ++PE V ++ N F L + +++ L + P G+T+ K FP+ EL++
Sbjct: 1019 SLSSEFKNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDK----RMWQSMCPLRQFRKLPE-EVVKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHLFPKLELSV 1188
BLAST of Helicase for meiosis 1 vs. Ensembl Human
Match: ASCC3 (activating signal cointegrator 1 complex subunit 3 [Source:HGNC Symbol;Acc:HGNC:18697]) HSP 1 Score: 83.5741 bits (205), Expect = 5.344e-26 Identity = 45/117 (38.46%), Postives = 65/117 (55.56%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
Q+ +++R G HHAG+ + DR +VE+ F G + VL T+TL+ GVNLPAH V+IKGT Q Y + ++Q+ GRAGRPQFD II+T+ ++
Sbjct: 764 QRSRNKQVRELFPDGFSIHHAGMLRQDRNLVENLFSNGHIKVLVCTATLAWGVNLPAHAVIIKGT--------QIYAAKRGSFVDLGILDVMQIFGRAGRPQFDKFGEGIIITTHDK 872 HSP 2 Score: 56.6102 bits (135), Expect = 5.344e-26 Identity = 31/109 (28.44%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGS--ICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
+L ++LNAEIAL + +++ KWI ++LY+R+ NP YG+ + I + + +L+I KL +I ++ T +T G + Y++ + TI+ +L
Sbjct: 893 SLADNLNAEIALGTVTNVEEAVKWISYTYLYVRMRANPLAYGISHKAYQIDPTLRKHREQLVIEVGRKLDKAQMIRFEERTGYFSSTDLGRTASHYYIKYNTIETFNEL 1001 HSP 3 Score: 100.908 bits (250), Expect = 3.213e-25 Identity = 56/150 (37.33%), Postives = 87/150 (58.00%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + +L FL + + L D+ +I+ + + L+ + G+G HHAGL + DR+ VE F+ + VL +TSTL+ GVN PAHLV+IKGT ++ + + +LQM+GRAGRPQFD+ A+IL +++F +F
Sbjct: 1568 TRLTALELIAFLATEEDPKQWLNMDEREMENIIA-TVRDSNLKLTLAFGIGMHHAGLHERDRKTVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKF 1716 HSP 4 Score: 36.965 bits (84), Expect = 3.213e-25 Identity = 26/102 (25.49%), Postives = 49/102 (48.04%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSIC-EAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L +HLNAEIA + +I ++ + R+ NP+YY + G + +++ + + LI + +L I + IE G + + Y+L QT++
Sbjct: 1731 LSDHLNAEIAGGTITSKQDALDYITWTYFFRRLIMNPSYYNL--GDVSHDSVNKFLSHLIEKSLIELELSYCIEIGEDNRSIEPLTYGRIASYYYLKHQTVK 1830
BLAST of Helicase for meiosis 1 vs. Ensembl Celegans
Match: Y54E2A.4 (pep chromosome:WBcel235:II:14754622:14778149:1 gene:WBGene00013189.1 transcript:Y54E2A.4a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y54E2A.4) HSP 1 Score: 99.7525 bits (247), Expect = 1.914e-32 Identity = 50/114 (43.86%), Postives = 75/114 (65.79%), Query Frame = 1
Query: 112 ISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAII-LTSSEEEFAHQF 444
I +E L+ + G+G HHAGL +R IVE F+ + VL +T+TL+ G+N PAHLV++KGT F+ + + +LQM+GRAGRPQFD+SAVA+I + +++ F +F
Sbjct: 1459 IKDENLKLTLPFGIGMHHAGLSAHERAIVEQLFIEKKIQVLIATATLAWGINCPAHLVIVKGTEYFDGKKGKYVDFPVTDVLQMMGRAGRPQFDDSAVAVIYVQDAKKTFYKKF 1572 HSP 2 Score: 60.8474 bits (146), Expect = 1.914e-32 Identity = 34/105 (32.38%), Postives = 60/105 (57.14%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRK 757
L H+NAEI+ + +I +++ ++LY R+F NP YYG+ S EA+ + I K++ V +L I+ D + I+ TPCG + + Y+L +T++ + K
Sbjct: 1587 LPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSE-EAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVRFLVK 1690 HSP 3 Score: 92.4337 bits (228), Expect = 1.598e-27 Identity = 48/97 (49.48%), Postives = 66/97 (68.04%), Query Frame = 1
Query: 145 RGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLL---QMIGRAGRPQFDNSAVAIILTSSEE 426
RG+G HHAGLC+ DR ++E F G + VL T+TL+ GVNLPAH VVIKGT F++ + +T +L Q+ GRAGRPQF+N II+T+ ++
Sbjct: 617 RGMGIHHAGLCRQDRILMERCFAEGHVSVLFCTATLAWGVNLPAHAVVIKGTDVFDAE-KGVFTDLGVLDVQQIFGRAGRPQFENEGHGIIITTRDK 712 HSP 4 Score: 51.9878 bits (123), Expect = 1.598e-27 Identity = 28/108 (25.93%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 431 LHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQI---IMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
+ L ++LNAE+AL + +D +W+ +++Y R +NP YG+ +I E P + +I +L +I D T + +T G + + +++ ++TIQ
Sbjct: 730 FYARLHDNLNAEVALGTVSTVDEGVEWLTYTYMYTRALKNPMAYGIAYNAI-ERDPNLRDHFGNVIREAAMQLDQNKMIRFDMATEYLNSTDLGRIASNFYVKYETIQ 836
BLAST of Helicase for meiosis 1 vs. Ensembl Celegans
Match: Y54E2A.4 (pep chromosome:WBcel235:II:14768462:14778149:1 gene:WBGene00013189.1 transcript:Y54E2A.4b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y54E2A.4) HSP 1 Score: 99.7525 bits (247), Expect = 2.484e-32 Identity = 50/114 (43.86%), Postives = 75/114 (65.79%), Query Frame = 1
Query: 112 ISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAII-LTSSEEEFAHQF 444
I +E L+ + G+G HHAGL +R IVE F+ + VL +T+TL+ G+N PAHLV++KGT F+ + + +LQM+GRAGRPQFD+SAVA+I + +++ F +F
Sbjct: 741 IKDENLKLTLPFGIGMHHAGLSAHERAIVEQLFIEKKIQVLIATATLAWGINCPAHLVIVKGTEYFDGKKGKYVDFPVTDVLQMMGRAGRPQFDDSAVAVIYVQDAKKTFYKKF 854 HSP 2 Score: 60.4622 bits (145), Expect = 2.484e-32 Identity = 33/101 (32.67%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L H+NAEI+ + +I +++ ++LY R+F NP YYG+ S EA+ + I K++ V +L I+ D + I+ TPCG + + Y+L +T++
Sbjct: 869 LPNHVNAEISAGTIDSKQAIVEYLSKTYLYRRLFANPNYYGLEEDSE-EAMLKFITKIVDDSVAELLASECIHVDSEQDVIKPTPCGRIASVYYLQHETVR 968 HSP 3 Score: 52.373 bits (124), Expect = 3.514e-6 Identity = 28/108 (25.93%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 431 LHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQI---IMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
+ L ++LNAE+AL + +D +W+ +++Y R +NP YG+ +I E P + +I +L +I D T + +T G + + +++ ++TIQ
Sbjct: 12 FYARLHDNLNAEVALGTVSTVDEGVEWLTYTYMYTRALKNPMAYGIAYNAI-ERDPNLRDHFGNVIREAAMQLDQNKMIRFDMATEYLNSTDLGRIASNFYVKYETIQ 118
BLAST of Helicase for meiosis 1 vs. Ensembl Celegans
Match: snrp-200 (Putative U5 small nuclear ribonucleoprotein 200 kDa helicase [Source:UniProtKB/Swiss-Prot;Acc:Q9U2G0]) HSP 1 Score: 88.9669 bits (219), Expect = 9.179e-30 Identity = 55/153 (35.95%), Postives = 85/153 (55.56%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKI----SEE----KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQ---NKLLQMIGRAGRPQFDNSAVAIILTSSEE 426
F +RK T + AK + D + + G S +I +E+ L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT +N + +T+ ++QM+GRAGRPQ+D+ I++T+ E
Sbjct: 717 FVHSRKETAKTAKAIRDACLEKDTLSAFMREGSASTEILRTEAEQAKNLDLKDLLPYGFAIHHAGMNRVDRTLVEDLFADRHIQVLFSTATLAWGVNLPAHTVIIKGTQIYNPE-KGRWTELGALDIMQMLGRAGRPQYDDRGEGILITNHSE 868 HSP 2 Score: 51.2174 bits (121), Expect = 9.179e-30 Identity = 29/109 (26.61%), Postives = 55/109 (50.46%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMK---LIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAE+ L + + W+ +FL++R+ +NP YG+ T A P + + LI + L LI +D+ + I+ T G + + ++ ++++Q KL
Sbjct: 890 LTDMLNAEVVLGTVSSVSEATNWLGYTFLFVRMLKNPTLYGI-THEQARADPLLEQRRADLIHTACVLLDKAGLIKYDKRSGIIQATELGRIASHFYCTYESMQTYNKL 997 HSP 3 Score: 31.9574 bits (71), Expect = 9.179e-30 Identity = 40/183 (21.86%), Postives = 74/183 (40.44%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINS--VMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELT 1394
S SE +++R E+ + + +H P P+K + AS K + L+Q + L+++ +L + + + R+ R + + + + L C K + W + HQ +I S V ++ N F L + Q L K P G+ + K FP+ E+T
Sbjct: 1012 SMSSEFKLLSVRDEEKLELQKMAEH-AP----IPIKENLDEASAKTNVLLQAYISQLKLEGFALQADMVFVAQSAGRLFRALFEIVLWRGWAGLAQKVLTLC----KMVTQRQW-GSLNPLHQFKKIPSEVVRSIDKKNYSFDRLYDLDQHQL----GDLIKMPKMGKPLFKFIRQFPKLEMT 1180 HSP 4 Score: 54.6842 bits (130), Expect = 3.806e-12 Identity = 33/114 (28.95%), Postives = 56/114 (49.12%), Query Frame = 1
Query: 112 ISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNS--NIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL-TSSEEEFAHQF 444
I +E LR + GVGF H G D IV+ F A+ V + + + A+LVV+ T +N ++ + Y +L M+G A RP D+ A +++ +S+ + +F
Sbjct: 1603 IEDESLRESVSCGVGFLHEGTAPKDVHIVQQLFESNAIQVCVVPRGMCYQIEMSAYLVVVMDTQFYNGKYHVYEDYPIADMLHMVGLANRPILDSDAKCVVMCQTSKRAYYKKF 1716 HSP 5 Score: 37.7354 bits (86), Expect = 3.806e-12 Identity = 24/103 (23.30%), Postives = 51/103 (49.51%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ + LY R+ +NP YY + G+ + + +L+ + L N I + ++++T G++ + Y++ +QTI+
Sbjct: 1731 LHDHFNAEIVTKTIENKQDAIDYLTWTLLYRRMTQNPNYYNL-QGTTHRHLSDALSELVELTLKDLENSKCI---AVKDEMDTVSLNLGMIASYYYISYQTIE 1829
BLAST of Helicase for meiosis 1 vs. Ensembl Celegans
Match: helq-1 (HELicase Q homolog [Source:UniProtKB/TrEMBL;Acc:Q65XX0]) HSP 1 Score: 83.1889 bits (204), Expect = 6.098e-16 Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAI-ILTSSEEE 429
L+ CI+ GV +HH+GL Q +R+ VE++F+ G + V+C+TSTL+ GVNLP V+IK + + + LQM GRAGR FD I I+ + EEE
Sbjct: 5 LKQCILSGVAYHHSGLTQDERKCVEAAFMEGLIYVVCATSTLAAGVNLPVRRVIIKAPMVGRERLGKA----QYLQMAGRAGRAGFDTKGDCITIIKAGEEE 102
BLAST of Helicase for meiosis 1 vs. Ensembl Celegans
Match: helq-1 (HELicase Q homolog [Source:UniProtKB/TrEMBL;Acc:Q65XX0]) HSP 1 Score: 82.8037 bits (203), Expect = 1.750e-15 Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAI-ILTSSEEE 429
L+ CI+ GV +HH+GL Q +R+ VE++F+ G + V+C+TSTL+ GVNLP V+IK + + + LQM GRAGR FD I I+ + EEE
Sbjct: 459 LKQCILSGVAYHHSGLTQDERKCVEAAFMEGLIYVVCATSTLAAGVNLPVRRVIIKAPMVGRERLGKA----QYLQMAGRAGRAGFDTKGDCITIIKAGEEE 556
BLAST of Helicase for meiosis 1 vs. Ensembl Fly
Match: l(3)72Ab (gene:FBgn0263599 transcript:FBtr0075527) HSP 1 Score: 93.2041 bits (230), Expect = 4.385e-32 Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 1
Query: 103 SQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
++++ +L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT +N + + L LQM+GRAGRPQ+D I++T+ E
Sbjct: 766 AEQVKNTELKELLPYGFAIHHAGMTRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGTQVYNPEKGRWVELSALDVLQMLGRAGRPQYDTKGEGILITNHSE 875 HSP 2 Score: 51.6026 bits (122), Expect = 4.385e-32 Identity = 34/109 (31.19%), Postives = 54/109 (49.54%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIP---QIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LYIR+ RNP YGV +I +A P Q L+ + L LI +D+ T + T G + + Y+L +T+ +L
Sbjct: 897 LPDMLNAEIVLGTVQHLQDAVNWLGYTYLYIRMLRNPTLYGVSHDAI-KADPLLEQHRADLLHTAACCLERSGLIKYDRKTGHFQVTDLGRIASHYYLTHETMLTYNQL 1004 HSP 3 Score: 35.8094 bits (81), Expect = 4.385e-32 Identity = 36/181 (19.89%), Postives = 82/181 (45.30%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELT 1394
S SE I++R E+ + L + ++ P+K I S K++ L+Q + L+++ +L + I + R++R + + + +TL C ++ + ++W + + Q ++ +A +L F R+ + + + ++ + P G+T+ K FP+ EL+
Sbjct: 1019 SLSSEFRHISVREEEKLELQKLME-----RVPIPIKESIEEHSAKVNVLLQAYISQLKLEGFALMSDMVFITQSAARLMRAIFEIVLTRGWAQLADKTLTLCKMIDR----RMWQSMTPL-RQFKKMPDEIAKKLEKKHFP-WGRLYDLEPHELGELI-RVPKLGKTIHKFVHQFPKLELS 1187 HSP 4 Score: 69.3218 bits (168), Expect = 1.597e-16 Identity = 38/116 (32.76%), Postives = 67/116 (57.76%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFN--SNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL-TSSEEEFAHQF 444
++++++ L+ + +GV + H GL D +VE F GA+ V + L G+++ AHLV+I T +N ++ + Y +LQMIGRA RP D A +++ SS+++F +F
Sbjct: 1601 ERMTDKTLKETLAQGVAYLHEGLSASDHRLVEQLFDSGAVQVAVISRDLCWGMSISAHLVIIMDTQFYNGKNHSYEDYPITDVLQMIGRANRPNEDADAKCVLMCQSSKKDFFKKF 1716 HSP 5 Score: 38.1206 bits (87), Expect = 1.597e-16 Identity = 23/101 (22.77%), Postives = 51/101 (50.50%), Query Frame = 2
Query: 449 EHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
+H NAE+ + + ++ +FLY R+ +NP YY + G + + +L+ + + L I+ + + ++T P G++ A Y++++ TI+
Sbjct: 1733 DHFNAEVVTKTIENKQDAVDYLTWTFLYRRLTQNPNYYNL-QGVTHRHLSDHLSELVENTLSDLEQSKCIS---VEDDMDTLPLNLGMIAAYYYINYTTIE 1829
BLAST of Helicase for meiosis 1 vs. Ensembl Fly
Match: obe (gene:FBgn0038344 transcript:FBtr0083147) HSP 1 Score: 116.316 bits (290), Expect = 1.140e-28 Identity = 57/122 (46.72%), Postives = 82/122 (67.21%), Query Frame = 1
Query: 88 QLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQ--NKLLQMIGRAGRPQFDNSAVAIILTSSEEE-FAHQF 444
++ +I Q I E+ L+ C+ G+G HHAGL + DR+ VE FL + +L +T+TL+ GVNLPAHLVVIKGT F+ +++ +LQM+GRAGRPQFDN VA++L E++ F +F
Sbjct: 1606 EIELILQNIREQNLKFCLAFGIGLHHAGLQEQDRKCVEELFLNRKIQILVATATLAWGVNLPAHLVVIKGTEYFDGKVKKYVDMPITDVLQMMGRAGRPQFDNEGVAVVLVHDEKKNFYKKF 1727 HSP 2 Score: 32.3426 bits (72), Expect = 1.140e-28 Identity = 13/40 (32.50%), Postives = 23/40 (57.50%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYY 562
L EH+NAEI + + ++ ++ + R+ RNP+YY
Sbjct: 1742 LPEHINAEIVAGTVQSKQAALDYLTWTYFFRRLLRNPSYY 1781 HSP 3 Score: 89.7373 bits (221), Expect = 6.409e-26 Identity = 44/95 (46.32%), Postives = 62/95 (65.26%), Query Frame = 1
Query: 148 GVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
G+ HHAG+ + DR++VE F+ G + VL T+TL+ GVNLPAH V+I+GT +++ L LQ+ GRAGRPQFD S V I+TS ++
Sbjct: 788 GLAMHHAGMLRADRQMVEKYFVEGHISVLVCTATLAWGVNLPAHAVIIRGTDIYDAKHGSFVDLGILDVLQIFGRAGRPQFDKSGVGTIITSYDK 882 HSP 4 Score: 49.2914 bits (116), Expect = 6.409e-26 Identity = 27/109 (24.77%), Postives = 56/109 (51.38%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMK---LIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L ++LNAEI L + ++D +W+ ++L++R+ NP YG+ + E P + + LI+S L ++ +Q T + T G + +++ + T++ +L
Sbjct: 904 LADNLNAEIGLGTITNVDEAIEWLSYTYLFVRMRINPHVYGIEYSEL-EKDPTLEARRRALIMSAAMSLDKARMMRFNQRTMDMNITDLGRTASYFYIKYDTVETFNEL 1011
BLAST of Helicase for meiosis 1 vs. Ensembl Fly
Match: tst (gene:FBgn0039117 transcript:FBtr0334895) HSP 1 Score: 72.4034 bits (176), Expect = 3.609e-12 Identity = 36/95 (37.89%), Postives = 54/95 (56.84%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL 411
L+ + RG+G HH+G+ + +EIVE F G + +L +T T +MGVN+PA VV F+ + + +QM GRAGR D + AII+
Sbjct: 574 LKDALERGIGVHHSGILPILKEIVEMLFQNGLVKLLFATETFAMGVNMPARTVVFDSCKKFDGLEMRNLKPGEYIQMAGRAGRRGHDETGTAIIM 668
BLAST of Helicase for meiosis 1 vs. Ensembl Fly
Match: tst (gene:FBgn0039117 transcript:FBtr0084462) HSP 1 Score: 72.4034 bits (176), Expect = 3.609e-12 Identity = 36/95 (37.89%), Postives = 54/95 (56.84%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL 411
L+ + RG+G HH+G+ + +EIVE F G + +L +T T +MGVN+PA VV F+ + + +QM GRAGR D + AII+
Sbjct: 574 LKDALERGIGVHHSGILPILKEIVEMLFQNGLVKLLFATETFAMGVNMPARTVVFDSCKKFDGLEMRNLKPGEYIQMAGRAGRRGHDETGTAIIM 668
BLAST of Helicase for meiosis 1 vs. Ensembl Fly
Match: DNApol-theta (gene:FBgn0002905 transcript:FBtr0082662) HSP 1 Score: 70.4774 bits (171), Expect = 1.924e-11 Identity = 49/110 (44.55%), Postives = 63/110 (57.27%), Query Frame = 1
Query: 94 GVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIK----GTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL 411
GV+S+ I+ AC FHHAGL +R+I+E+SF GAL VL +TSTLS GVNLPA V+I+ G +S TY QMIGRAGR D +I++
Sbjct: 534 GVMSKAIT----YAC-----AFHHAGLTTEERDIIEASFKAGALKVLVATSTLSSGVNLPARRVLIRSPLFGGKQMSS---LTYR-----QMIGRAGRMGKDTLGESILI 626
BLAST of Helicase for meiosis 1 vs. Ensembl Zebrafish
Match: hfm1 (helicase for meiosis 1 [Source:ZFIN;Acc:ZDB-GENE-061207-43]) HSP 1 Score: 176.407 bits (446), Expect = 3.352e-98 Identity = 100/313 (31.95%), Postives = 172/313 (54.95%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQS--EVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNY--LNYIESQTKISKQNPEKF 1766
I IS+ E S++ LR ER +N LNK + +RFP++G+I + MK++CL+Q LGC+ + + L Q+T KIFR +R+ R + + FL N+ ++ +L KCF AKLW+N+ ++S Q+ I +A L NAG +T ++I + + +E I+N+ PPFG + + S PR E+++EQI + + VF M + + D + + LI+GD +N V ++ +I + L+ S W I V R + L I LIS +VG DI+ + +Y ++ ++++ I+++ +F
Sbjct: 756 IEMISKAKEFSDMQLRVNERKTLNTLNKDKNRTTIRFPIEGKIKSNDMKVNCLIQAQLGCIPIQEFGLIQDTGKIFRNAVRVSRYLTE--FLCHHSKSNFAAQLNALILAKCFRAKLWENSPYISKQLERIGLSLATALVNAGLTTFSKIEQTSPRELELIVNRHPPFGNQIKEAISKLPRCEVSLEQIPRYSPSTAEI-VFTVNMNNFKELALKRTAPDHHY----VTLIVGDCDNKVVFQQKISDTLLLKSGSWSKRIEVTRSPKGEELSIHLISSEYVGLDIQQDYSAHYSGVHGLQAEFSITEKPTWRF 1061 HSP 2 Score: 139.813 bits (351), Expect = 3.352e-98 Identity = 68/148 (45.95%), Postives = 96/148 (64.86%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEFAH 438
FC TRK Q A L RF + D +L + + + KLR + G+G+HHAG+ DR+I E++F G LPVL +TSTL+MGVN+PAHLVVIK T ++ + Y++ LLQMIGRAGRPQFD +A A+I+T +++++ H
Sbjct: 495 FCSTRKGVQQSASVLAKDARFIMSIDHKQRLVKYANSLLDAKLRDLFVYGIGYHHAGMDVSDRKITENAFTVGDLPVLFTTSTLAMGVNMPAHLVVIKSTMHYVGGACEEYSEADLLQMIGRAGRPQFDTTATAVIMTRPQTKDKYMH 642 HSP 3 Score: 86.6557 bits (213), Expect = 3.352e-98 Identity = 47/121 (38.84%), Postives = 71/121 (58.68%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH NL+EHLNAEI LH + D++ WI+S+FLYIR +NP +Y +L + ++ L++ +LI D+ N I+ T G LMARY + F T++ ++G+ETL L+
Sbjct: 653 SLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPKHY----------------ELCLKNLNSLASFNLITMDEDIN-IKPTETGKLMARYCVAFDTVKQFSMVTGTETLPELI 756
BLAST of Helicase for meiosis 1 vs. Ensembl Zebrafish
Match: ascc3 (activating signal cointegrator 1 complex subunit 3 [Source:NCBI gene;Acc:100332599]) HSP 1 Score: 94.3597 bits (233), Expect = 8.255e-30 Identity = 57/152 (37.50%), Postives = 83/152 (54.61%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVD--QTRFELKFDQIHQLGVISQ------KISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
F R ST + A L++ + R EL F Q+ Q Q + +++R G G HHAG+ + DR ++ES F G L VL T+TL+ GVNLPAH V+IKGT+ +++ L +Q+ GRAGRPQFD I+T+ ++
Sbjct: 719 FVHARNSTVRTAMSLIEMAKNRGELSFFQVDQGADYGQCEKQIQRSRNKQMREMFPDGFGIHHAGMLRQDRSLMESMFSRGYLKVLVCTATLAWGVNLPAHAVIIKGTNIYDAKRGTLVDLGILDVMQIFGRAGRPQFDKYGEGTIITTHDK 870 HSP 2 Score: 58.5362 bits (140), Expect = 8.255e-30 Identity = 30/114 (26.32%), Postives = 61/114 (53.51%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIM---KLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSE 772
+L ++LNAEIAL + ++D +W+ ++LY+R+ NP YG+ + PQ+ + +L++ KL +I D+ T +T G + +++ + TI+ +L ++
Sbjct: 891 SLADNLNAEIALGTVTNVDEAVRWLSYTYLYVRMRANPLAYGI-NHKAYQMDPQLELYRKELVVESGRKLDKARMIRFDERTGYFASTDLGRTASHFYIKYNTIESFNELFNAQ 1003 HSP 3 Score: 104.375 bits (259), Expect = 8.085e-27 Identity = 59/150 (39.33%), Postives = 86/150 (57.33%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L FL D + L D+ +I+ I E L+ + G+G HHAGL + DR+ VE F+ + VL +TSTL+ GVN PAHLV++KGT ++ + Y +LQM+GRAGRPQFD+ A+IL +++F +F
Sbjct: 1566 TRLTALDLIAFLATEDDPKQWLHQDEREMTDIIA-TIRESNLKLTLAFGIGMHHAGLHERDRKTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEYYDGKTRRYVDYPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKF 1714 HSP 4 Score: 38.1206 bits (87), Expect = 8.085e-27 Identity = 28/101 (27.72%), Postives = 46/101 (45.54%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L +HLNAEIA + +I ++ + R+ NP+YY + S E I + + L+ + L + Q IE G + + Y+L QTI+
Sbjct: 1729 LSDHLNAEIAAGTVTSKQDAMDYITWTYFFRRLVMNPSYYNLDDIS-HETINKYLSNLVERSLRDLECSYCMEIQQDEQTIEPLTYGRISSYYYLKHQTIR 1828
BLAST of Helicase for meiosis 1 vs. Ensembl Zebrafish
Match: snrnp200 (small nuclear ribonucleoprotein 200 (U5) [Source:ZFIN;Acc:ZDB-GENE-081105-64]) HSP 1 Score: 89.3521 bits (220), Expect = 8.998e-30 Identity = 47/111 (42.34%), Postives = 65/111 (58.56%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D+ I++TS E
Sbjct: 770 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDSKGEGILITSHGE 872 HSP 2 Score: 50.0618 bits (118), Expect = 8.998e-30 Identity = 28/108 (25.93%), Postives = 54/108 (50.00%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LY+R+ RNP YGV SI + + M L+ + L +L+ +D+ + + T G + + +++ ++I +L
Sbjct: 894 LADMLNAEIVLGNVQNAKDAVNWLGYTYLYVRMLRNPTLYGVSHDDRSIDPLLERRRMDLVHTAATVLEKNNLVKYDKRSGSFQVTDLGRIASHFYITHESIMTYNQL 1001 HSP 3 Score: 33.113 bits (74), Expect = 8.998e-30 Identity = 37/184 (20.11%), Postives = 80/184 (43.48%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +TL C ++ K ++W + + ++PE + ++ N F L + +++ L + P G+T+ K FP+ +L +
Sbjct: 1016 SLSSEFRNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAYISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLSRGWAQLTDKTLNLCKMIDK----RMWQSMSPLRQFRKLPE-EVIKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHQFPKLDLAV 1185 HSP 4 Score: 73.9442 bits (180), Expect = 1.300e-17 Identity = 40/116 (34.48%), Postives = 64/116 (55.17%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEEFAHQF 444
+ +S+ L+ + GVG+ H GL +R IVE F GA+ V+ ++ +L G N+ AHLV++ T +N I Y +LQM+G+A RP Q D I+ S+++F +F
Sbjct: 1598 ENLSDVTLKETLSNGVGYLHEGLSPTERRIVEHLFTSGAIQVVVASRSLCWGTNISAHLVIVMDTQYYNGKIHAYVDYPIYDVLQMVGKANRPLQDDEGRCVIMCQGSKKDFFKKF 1713 HSP 5 Score: 37.3502 bits (85), Expect = 1.300e-17 Identity = 25/103 (24.27%), Postives = 52/103 (50.49%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1728 LHDHFNAEIVTKTVENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGMSHRHLSDHLSELVENTLQDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1826
BLAST of Helicase for meiosis 1 vs. Ensembl Zebrafish
Match: snrnp200 (small nuclear ribonucleoprotein 200 (U5) [Source:ZFIN;Acc:ZDB-GENE-081105-64]) HSP 1 Score: 89.3521 bits (220), Expect = 8.998e-30 Identity = 47/111 (42.34%), Postives = 65/111 (58.56%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D+ I++TS E
Sbjct: 770 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDSKGEGILITSHGE 872 HSP 2 Score: 50.0618 bits (118), Expect = 8.998e-30 Identity = 28/108 (25.93%), Postives = 54/108 (50.00%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LY+R+ RNP YGV SI + + M L+ + L +L+ +D+ + + T G + + +++ ++I +L
Sbjct: 894 LADMLNAEIVLGNVQNAKDAVNWLGYTYLYVRMLRNPTLYGVSHDDRSIDPLLERRRMDLVHTAATVLEKNNLVKYDKRSGSFQVTDLGRIASHFYITHESIMTYNQL 1001 HSP 3 Score: 33.113 bits (74), Expect = 8.998e-30 Identity = 37/184 (20.11%), Postives = 80/184 (43.48%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +TL C ++ K ++W + + ++PE + ++ N F L + +++ L + P G+T+ K FP+ +L +
Sbjct: 1016 SLSSEFRNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAYISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLSRGWAQLTDKTLNLCKMIDK----RMWQSMSPLRQFRKLPE-EVIKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHQFPKLDLAV 1185 HSP 4 Score: 73.9442 bits (180), Expect = 1.300e-17 Identity = 40/116 (34.48%), Postives = 64/116 (55.17%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEEFAHQF 444
+ +S+ L+ + GVG+ H GL +R IVE F GA+ V+ ++ +L G N+ AHLV++ T +N I Y +LQM+G+A RP Q D I+ S+++F +F
Sbjct: 1598 ENLSDVTLKETLSNGVGYLHEGLSPTERRIVEHLFTSGAIQVVVASRSLCWGTNISAHLVIVMDTQYYNGKIHAYVDYPIYDVLQMVGKANRPLQDDEGRCVIMCQGSKKDFFKKF 1713 HSP 5 Score: 37.3502 bits (85), Expect = 1.300e-17 Identity = 25/103 (24.27%), Postives = 52/103 (50.49%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1728 LHDHFNAEIVTKTVENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGMSHRHLSDHLSELVENTLQDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1826
BLAST of Helicase for meiosis 1 vs. Ensembl Zebrafish
Match: sclt1 (sodium channel and clathrin linker 1 [Source:ZFIN;Acc:ZDB-GENE-030131-3278]) HSP 1 Score: 100.908 bits (250), Expect = 5.930e-21 Identity = 154/677 (22.75%), Postives = 331/677 (48.89%), Query Frame = 1
Query: 3022 SEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIK-------LIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRN-QIDNMPKNFETCFSEQLKV---------NMQAQIDLMIKEKNSLQEMLRISELEVDRLQNE------------VTENNMKPQTV----------IFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKI-------------QADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNXXXXXXXXXXXXXLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQ--MSCDKAQSNADRFTKLLLASEESATKN-----RKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFXXXXXXXXXXWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLERKVNISEDMAP 4875
+E +FL+DQV+RLN VL YQ+++ S D+ L PL+ EYD+ I +M +++ ++ + + S++ + KENERL Q ++ K ++ + N++ Q+ ++EK + +M + + E+DRLQ + V +M+ Q + N++++S Q ++ ++ E E D++R L Q+ ++E+ N++ A + + +EA++ L QL+++L +++ Q E+V +T+ + C++L D EK ++A ++ + ++AE+A+++++ ++RE Q E E+++ + ++++A +RT++EV+ V + ++ R+ +EL+ + + ++++ R + +E+E+E +++ R + E + H R + AE+ ++E +L L S N + E++ E S Q+++ LQ ++ + +S A +L L E RK+ + R + + ++Q +K+Q LE+RL E++ +++++ + + L QQ+ S++++EA NL++ ++QKL + E +RQK+R E+ + Q+ILE + EQ+ L+R+++ +E A
Sbjct: 3 TEVEFLRDQVNRLNSVLGRYQDEFPPPSSVSQVEDVVIEAAAPWLADRGLMAPLIEEYDRHIQQMEMQLKFYQRQMTDVKASLEQVVKENERLHAEQRLSVEKQLQSLSAGAEGDAAADAMAISNLEEQVKCAVEEKERVLQMWQTASQELDRLQTQFQSSMSDGQIHAVERQHMQSQLAQLQQHAQKLQLTNQKLDSTNQQFVKTVAEQSAEL---EDLRRQLRQAKLDQRSAATKVEEMNRLVQNVQDQMQRREEDAAEAQGREEASDRRLQQLQAALSQLEARLKSATQDCESVRRKQTDCERQVGELQGRCSSLED------EK----FEALSRLRSSIQLAEEASLQREQAQLREKQRAEELEKMKDAMKQLIQNAAMRTRKEVESVRKQCNVQIHRMAEELSALQLECADKETQIERAHRERKAVEEELEKVYKEGRVGEPEMRKMEAL-HQRCLNAERLKDETQLALNSMNNKMKKMEMEYS-------EELSRCQEEVRRLQSALTTARQESTAVSEERLTLQQENQQMHRDMEALRKECALAQRRAKQQVSSMQQELCVKEQGLESRLREMEESSKNSSAGLTRLLLAQQKTISRYKDEAKNLTQAFQQKLNSLRSELNRQKQRVQELEIQMEADHQKILEFERQVSEQQEKNTRLQRRLSQAEQRAA 658
BLAST of Helicase for meiosis 1 vs. Ensembl Xenopus
Match: hfm1 (HFM1, ATP-dependent DNA helicase homolog [Source:NCBI gene;Acc:779837]) HSP 1 Score: 199.134 bits (505), Expect = 5.881e-111 Identity = 100/295 (33.90%), Postives = 169/295 (57.29%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCS--LHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLN 1718
+S IS CSE S++ LR ER +N LNK + +R+P++G+I MK++CL+Q LGC+ V D SL Q+ KIFR G R+ +C+ ++L L + + ++ +L KCF +KLW+N+ +S Q+ +I +A + NAG +T +I ++ A+ +E I+N+ PPFG + + + P+YE+ +Q+ + + L V +T +Q + D F ++L+IGD +N +K +IM+ L + W +I + R S+ +C++LIS +VG DI+ F YL
Sbjct: 745 VSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQE---NKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAP-DSHF----VMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLG 1031 HSP 2 Score: 150.984 bits (380), Expect = 5.881e-111 Identity = 72/151 (47.68%), Postives = 103/151 (68.21%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEFAHQFD 447
FC TRK Q A L +F + + +L + I + KLR + GVG+HHAG+ DR+++E+SFL G LPVL +TSTL+MGVNLPAHLV++K T ++ S + Q Y++ +LQMIGRAGRPQFD++A A+I+T S++E++ H D
Sbjct: 468 FCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLD 618 HSP 3 Score: 96.2857 bits (238), Expect = 5.881e-111 Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +L+EHLNAEIALH + D+ +WI+S+FLYIR +NPAYYG G I + +L + ++ LS+ LI D+ N + T G LMA Y++ F T + + G+ETL LV
Sbjct: 626 SLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEIN-FKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELV 745
BLAST of Helicase for meiosis 1 vs. Ensembl Xenopus
Match: SNRNP200 (small nuclear ribonucleoprotein, U5 200kDa subunit [Source:NCBI gene;Acc:100145098]) HSP 1 Score: 89.3521 bits (220), Expect = 2.159e-28 Identity = 47/111 (42.34%), Postives = 64/111 (57.66%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D I++TS E
Sbjct: 712 ELKDLLPYGFAIHHAGMSRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 814 HSP 2 Score: 45.8246 bits (107), Expect = 2.159e-28 Identity = 28/109 (25.69%), Postives = 53/109 (48.62%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAE+ L + + W+ ++LYIR+ R+P YG+ I + Q + LI + L +L+ +D+ T + T G + + +++ +IQ +L
Sbjct: 835 KLPDMLNAEVVLGHVQNAKDAVNWLGYTYLYIRMLRSPNLYGISHDDLKIDPLLEQRRLDLIHTAALLLDKNNLMKYDKKTGNFQVTELGRIASHFYITNDSIQTYNQL 943 HSP 3 Score: 33.4982 bits (75), Expect = 2.159e-28 Identity = 38/184 (20.65%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +TL C ++ K ++W + + ++PE V ++ N F L + +++ L + P G+T+ K FP+ +L++
Sbjct: 958 SLSSEFKNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDK----RMWQSMCPLRQFRKLPE-EVVKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHLFPKLDLSV 1127 HSP 4 Score: 82.4185 bits (202), Expect = 8.179e-20 Identity = 44/116 (37.93%), Postives = 68/116 (58.62%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEEFAHQF 444
+K+S+E L+ ++ GVG+ H GL L+R IVE F GA+ V+ ++ +L G+N+ AHLV+I T +N I Y +LQM+G A RP Q D I+ S+++F +F
Sbjct: 1541 EKLSDETLKETLLNGVGYLHEGLSSLERRIVEQLFNTGAVQVIVASRSLCWGLNIAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGYANRPLQDDEGRCVIMCQGSKKDFFKKF 1656 HSP 5 Score: 37.3502 bits (85), Expect = 8.179e-20 Identity = 25/103 (24.27%), Postives = 51/103 (49.51%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1671 LHDHFNAEIVTKTVENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGVSHRHLSDHLSELVEHTLSDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1769
BLAST of Helicase for meiosis 1 vs. Ensembl Xenopus
Match: gipc1 (GIPC PDZ domain containing family member 1 [Source:Xenbase;Acc:XB-GENE-493927]) HSP 1 Score: 83.5741 bits (205), Expect = 2.859e-25 Identity = 44/109 (40.37%), Postives = 64/109 (58.72%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
Q+ ++LR G HHAG+ + DR +VE+ F G + VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQFD I+T+ ++
Sbjct: 771 QRSRNKQLRELFPDGFSIHHAGMLRQDRSLVENLFSQGHIKVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGSFVDLGILDVMQIFGRAGRPQFDKFGEGTIITTHDK 879 HSP 2 Score: 54.6842 bits (130), Expect = 2.859e-25 Identity = 28/109 (25.69%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
+L ++LNAE+AL + +++ KW+ ++LY+R+ NP YG+ + A+ + +L+I KL +I ++ T +T G + +++ + TI+ +L
Sbjct: 900 SLADNLNAEVALGTVTNVEEAVKWLSYTYLYVRMRANPLVYGISHKVYQMDPALEKHREQLVIEAGRKLDKARMIRFEERTGFFSSTDLGRTSSHFYIKYNTIETFNEL 1008 HSP 3 Score: 100.138 bits (248), Expect = 5.085e-25 Identity = 56/150 (37.33%), Postives = 85/150 (56.67%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L FL + + L D+ ++ I + + L+ + G+G HHAGL + DR VE F+ + VL +TSTL+ GVN PAHLV++KGT ++ + Y +LQM+GRAGRPQFD+ A+IL +++F +F
Sbjct: 1575 TRLTALDLIAFLATENDPKQWLNMDE-REMNDIIGTLKDSNLKLTLAFGIGMHHAGLHERDRRTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEFYDGKTRRYVDYPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKF 1723 HSP 4 Score: 36.965 bits (84), Expect = 5.085e-25 Identity = 25/101 (24.75%), Postives = 46/101 (45.54%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L +HLNAEIA + +I ++ + R+ NP+YY + E++ + + L+ + L I + IE G + + Y+L QT++
Sbjct: 1738 LSDHLNAEIAAGTITSKQDAMDYITWTYFFRRLMMNPSYYNLEDVG-HESMNKFLSNLVEKSLIDLECSYCIEIAEDNRSIEPQTYGRIASYYYLKHQTVR 1837
BLAST of Helicase for meiosis 1 vs. Ensembl Xenopus
Match: gipc1 (GIPC PDZ domain containing family member 1 [Source:Xenbase;Acc:XB-GENE-493927]) HSP 1 Score: 83.5741 bits (205), Expect = 2.907e-25 Identity = 44/109 (40.37%), Postives = 64/109 (58.72%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
Q+ ++LR G HHAG+ + DR +VE+ F G + VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQFD I+T+ ++
Sbjct: 762 QRSRNKQLRELFPDGFSIHHAGMLRQDRSLVENLFSQGHIKVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGSFVDLGILDVMQIFGRAGRPQFDKFGEGTIITTHDK 870 HSP 2 Score: 54.6842 bits (130), Expect = 2.907e-25 Identity = 28/109 (25.69%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
+L ++LNAE+AL + +++ KW+ ++LY+R+ NP YG+ + A+ + +L+I KL +I ++ T +T G + +++ + TI+ +L
Sbjct: 891 SLADNLNAEVALGTVTNVEEAVKWLSYTYLYVRMRANPLVYGISHKVYQMDPALEKHREQLVIEAGRKLDKARMIRFEERTGFFSSTDLGRTSSHFYIKYNTIETFNEL 999 HSP 3 Score: 100.138 bits (248), Expect = 5.003e-25 Identity = 56/150 (37.33%), Postives = 85/150 (56.67%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L FL + + L D+ ++ I + + L+ + G+G HHAGL + DR VE F+ + VL +TSTL+ GVN PAHLV++KGT ++ + Y +LQM+GRAGRPQFD+ A+IL +++F +F
Sbjct: 1566 TRLTALDLIAFLATENDPKQWLNMDE-REMNDIIGTLKDSNLKLTLAFGIGMHHAGLHERDRRTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEFYDGKTRRYVDYPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKF 1714 HSP 4 Score: 36.965 bits (84), Expect = 5.003e-25 Identity = 25/101 (24.75%), Postives = 46/101 (45.54%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L +HLNAEIA + +I ++ + R+ NP+YY + E++ + + L+ + L I + IE G + + Y+L QT++
Sbjct: 1729 LSDHLNAEIAAGTITSKQDAMDYITWTYFFRRLMMNPSYYNLEDVG-HESMNKFLSNLVEKSLIDLECSYCIEIAEDNRSIEPQTYGRIASYYYLKHQTVR 1828
BLAST of Helicase for meiosis 1 vs. Ensembl Xenopus
Match: gipc1 (GIPC PDZ domain containing family member 1 [Source:Xenbase;Acc:XB-GENE-493927]) HSP 1 Score: 83.5741 bits (205), Expect = 2.980e-25 Identity = 44/109 (40.37%), Postives = 64/109 (58.72%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
Q+ ++LR G HHAG+ + DR +VE+ F G + VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQFD I+T+ ++
Sbjct: 747 QRSRNKQLRELFPDGFSIHHAGMLRQDRSLVENLFSQGHIKVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGSFVDLGILDVMQIFGRAGRPQFDKFGEGTIITTHDK 855 HSP 2 Score: 54.6842 bits (130), Expect = 2.980e-25 Identity = 28/109 (25.69%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
+L ++LNAE+AL + +++ KW+ ++LY+R+ NP YG+ + A+ + +L+I KL +I ++ T +T G + +++ + TI+ +L
Sbjct: 876 SLADNLNAEVALGTVTNVEEAVKWLSYTYLYVRMRANPLVYGISHKVYQMDPALEKHREQLVIEAGRKLDKARMIRFEERTGFFSSTDLGRTSSHFYIKYNTIETFNEL 984 HSP 3 Score: 100.138 bits (248), Expect = 5.432e-25 Identity = 56/150 (37.33%), Postives = 85/150 (56.67%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L FL + + L D+ ++ I + + L+ + G+G HHAGL + DR VE F+ + VL +TSTL+ GVN PAHLV++KGT ++ + Y +LQM+GRAGRPQFD+ A+IL +++F +F
Sbjct: 1551 TRLTALDLIAFLATENDPKQWLNMDE-REMNDIIGTLKDSNLKLTLAFGIGMHHAGLHERDRRTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEFYDGKTRRYVDYPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKF 1699 HSP 4 Score: 36.965 bits (84), Expect = 5.432e-25 Identity = 25/101 (24.75%), Postives = 46/101 (45.54%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L +HLNAEIA + +I ++ + R+ NP+YY + E++ + + L+ + L I + IE G + + Y+L QT++
Sbjct: 1714 LSDHLNAEIAAGTITSKQDAMDYITWTYFFRRLMMNPSYYNLEDVG-HESMNKFLSNLVEKSLIDLECSYCIEIAEDNRSIEPQTYGRIASYYYLKHQTVR 1813
BLAST of Helicase for meiosis 1 vs. Ensembl Mouse
Match: Hfm1 (HFM1, ATP-dependent DNA helicase homolog [Source:MGI Symbol;Acc:MGI:3036246]) HSP 1 Score: 184.111 bits (466), Expect = 9.023e-103 Identity = 111/314 (35.35%), Postives = 167/314 (53.18%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHV-FVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNR--ISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTN 1772
IS IS C+E ++ LR +E+ +N LNK +RFP+ RI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D+ +++ + L++ +L KCF KLW+N+ VS Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + + P+YEL +EQI + V + R ++ D + LIIGD +N V +K +IM+ L+ S W +I V R ISED L I+LIS +VG DI KF Y P KF N
Sbjct: 808 ISMISSCNEFLDVQLRISEKRILNTLNKDPNRITIRFPMAERIKTREMKVNCLIQAQLGCIPIQDFALTQDTVKIFRNGSRIARWLSDF---VAAQEKKFAVLLNSVILTKCFKCKLWENSKHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVAHLPKYELEVEQIARYSDIKAEILVTIILRNFEQLQTKRTAPDFHYAT----LIIGDADNQVVFKHKIMDSVLLKSGNWVKKIDVKRALISED--LSINLISSDYVGLDIHQKFTVFYF------------GPRKFVN 1100 HSP 2 Score: 140.198 bits (352), Expect = 9.023e-103 Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEF 432
FC TRK Q A LV +F + +Q +L + I + KL+ ++ GVG+HHAG+ DR++VE F G LPVL +TSTL+MG+N+PAHLVVIK T +++ + + Y++ +LQMIGRAGRPQFD +A A+I+T S+ E++
Sbjct: 531 FCSTRKGVQQAASVLVKDAKFIISVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEGLFTSGDLPVLFTTSTLAMGMNMPAHLVVIKSTMHYSGGVFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKY 676 HSP 3 Score: 93.9745 bits (232), Expect = 9.023e-103 Identity = 49/121 (40.50%), Postives = 74/121 (61.16%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ WI+S+ LYIR +NP++YG +G + I + +L + + LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL L+
Sbjct: 689 SLHRHLIEHLNAEIVLHTITDVNIALDWIRSTMLYIRALKNPSHYGFSSGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDVN-FKPTEAGRLMAWYYITFETVKKFCAISGKETLLDLI 808
BLAST of Helicase for meiosis 1 vs. Ensembl Mouse
Match: Hfm1 (HFM1, ATP-dependent DNA helicase homolog [Source:MGI Symbol;Acc:MGI:3036246]) HSP 1 Score: 184.111 bits (466), Expect = 9.023e-103 Identity = 111/314 (35.35%), Postives = 167/314 (53.18%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHV-FVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNR--ISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTN 1772
IS IS C+E ++ LR +E+ +N LNK +RFP+ RI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D+ +++ + L++ +L KCF KLW+N+ VS Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + + P+YEL +EQI + V + R ++ D + LIIGD +N V +K +IM+ L+ S W +I V R ISED L I+LIS +VG DI KF Y P KF N
Sbjct: 808 ISMISSCNEFLDVQLRISEKRILNTLNKDPNRITIRFPMAERIKTREMKVNCLIQAQLGCIPIQDFALTQDTVKIFRNGSRIARWLSDF---VAAQEKKFAVLLNSVILTKCFKCKLWENSKHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVAHLPKYELEVEQIARYSDIKAEILVTIILRNFEQLQTKRTAPDFHYAT----LIIGDADNQVVFKHKIMDSVLLKSGNWVKKIDVKRALISED--LSINLISSDYVGLDIHQKFTVFYF------------GPRKFVN 1100 HSP 2 Score: 140.198 bits (352), Expect = 9.023e-103 Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEF 432
FC TRK Q A LV +F + +Q +L + I + KL+ ++ GVG+HHAG+ DR++VE F G LPVL +TSTL+MG+N+PAHLVVIK T +++ + + Y++ +LQMIGRAGRPQFD +A A+I+T S+ E++
Sbjct: 531 FCSTRKGVQQAASVLVKDAKFIISVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEGLFTSGDLPVLFTTSTLAMGMNMPAHLVVIKSTMHYSGGVFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKY 676 HSP 3 Score: 93.9745 bits (232), Expect = 9.023e-103 Identity = 49/121 (40.50%), Postives = 74/121 (61.16%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ WI+S+ LYIR +NP++YG +G + I + +L + + LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL L+
Sbjct: 689 SLHRHLIEHLNAEIVLHTITDVNIALDWIRSTMLYIRALKNPSHYGFSSGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDVN-FKPTEAGRLMAWYYITFETVKKFCAISGKETLLDLI 808
BLAST of Helicase for meiosis 1 vs. Ensembl Mouse
Match: Hfm1 (HFM1, ATP-dependent DNA helicase homolog [Source:MGI Symbol;Acc:MGI:3036246]) HSP 1 Score: 142.124 bits (357), Expect = 1.531e-37 Identity = 100/316 (31.65%), Postives = 145/316 (45.89%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKL--RFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHV-FVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNR--ISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTN 1772
IS IS C+E ++ LR +E+ +N LNK PN++ RFP+ RI MK++CL+Q LGC+ + D +L Q+T KIFR G RI RC+ ++ + NAG ++ +I + A+ +E ILN+ PPFG + + + P+YEL +EQI + V + R ++ D + LIIGD +N V +K +IM+ L+ S W +I V R ISED L I+LIS +VG DI KF Y P KF N
Sbjct: 66 ISMISSCNEFLDVQLRISEKRILNTLNKD--PNRITIRFPMAERIKTREMKVNCLIQAQLGCIPIQDFALTQDTVKIFRNGSRIARCIS-----------------------------------------------LSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVAHLPKYELEVEQIARYSDIKAEILVTIILRNFEQLQTKRTAPDFHYAT----LIIGDADNQVVFKHKIMDSVLLKSGNWVKKIDVKRALISED--LSINLISSDYVGLDIHQKFTVFYF------------GPRKFVN 314 HSP 2 Score: 36.5798 bits (83), Expect = 1.531e-37 Identity = 22/66 (33.33%), Postives = 36/66 (54.55%), Query Frame = 2
Query: 593 IPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
I + +L + + LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL L+
Sbjct: 2 IEAKLQELCLKNLKDLSSLDLIKMDEDVN-FKPTEAGRLMAWYYITFETVKKFCAISGKETLLDLI 66
BLAST of Helicase for meiosis 1 vs. Ensembl Mouse
Match: Snrnp200 (small nuclear ribonucleoprotein 200 (U5) [Source:MGI Symbol;Acc:MGI:2444401]) HSP 1 Score: 89.3521 bits (220), Expect = 8.438e-30 Identity = 45/104 (43.27%), Postives = 66/104 (63.46%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQ---NKLLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT ++ + +T+ +LQM+GRAGRPQ+D I++TS E
Sbjct: 773 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPE-KGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 875 HSP 2 Score: 48.9062 bits (115), Expect = 8.438e-30 Identity = 28/108 (25.93%), Postives = 53/108 (49.07%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSIC--EAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LYIR+ R+P YG+ + + Q + L+ + L +L+ +D+ T + T G + + Y++ T+Q +L
Sbjct: 897 LPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQL 1004 HSP 3 Score: 34.6538 bits (78), Expect = 8.438e-30 Identity = 39/184 (21.20%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +TL C ++ K ++W + + ++PE V ++ N F L + +++ L + P G+T+ K FP+ EL++
Sbjct: 1019 SLSSEFKNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDK----RMWQSMCPLRQFRKLPE-EVVKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHLFPKLELSV 1188 HSP 4 Score: 78.5666 bits (192), Expect = 1.018e-18 Identity = 41/116 (35.34%), Postives = 67/116 (57.76%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEEFAHQF 444
+K+S+ L+ ++ GVG+ H GL ++R +VE F GA+ V+ ++ +L G+N+ AHLV+I T +N I Y +LQM+G A RP Q D I+ S+++F +F
Sbjct: 1602 EKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKF 1717 HSP 5 Score: 36.965 bits (84), Expect = 1.018e-18 Identity = 24/101 (23.76%), Postives = 50/101 (49.50%), Query Frame = 2
Query: 449 EHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
+H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1734 DHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGISHRHLSDHLSELVEQTLSDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1830
BLAST of Helicase for meiosis 1 vs. Ensembl Mouse
Match: Ascc3 (activating signal cointegrator 1 complex subunit 3 [Source:MGI Symbol;Acc:MGI:1925237]) HSP 1 Score: 100.523 bits (249), Expect = 1.682e-26 Identity = 56/150 (37.33%), Postives = 87/150 (58.00%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + +L FL + + L D+ ++ I + + L+ + G+G HHAGL + DR+ VE F+ + VL +TSTL+ GVN PAHLV+IKGT ++ + + +LQM+GRAGRPQFD+ A+IL +++F +F
Sbjct: 1569 TRLTALELIAFLATEEDPKQWLNMDE-QEMDNIIGTVRDSNLKLTLAFGIGMHHAGLHERDRKTVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFPITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKF 1717 HSP 2 Score: 41.2022 bits (95), Expect = 1.682e-26 Identity = 27/102 (26.47%), Postives = 50/102 (49.02%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICE-AIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
L +HLNAEIA + +I ++ + R+ NP+YY + G + + +I + + LI + +L I + IE CG + + Y+L +T++
Sbjct: 1732 LSDHLNAEIAGGTITSKQDAMDYITWTYFFRRLIMNPSYYSL--GDVSQDSINKFLSHLIGQSLVELELSHCIEVGEDNRTIEPLTCGRIASYYYLKHKTVK 1831 HSP 3 Score: 85.1149 bits (209), Expect = 1.741e-25 Identity = 47/117 (40.17%), Postives = 65/117 (55.56%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
QK +++R G HHAG+ + DR +VE+ F G + VL T+TL+ GVNLPAH VVIKGT Q Y + ++Q+ GRAGRPQFD II+T+ ++
Sbjct: 765 QKSRNKQVRELFSDGFSIHHAGMLRQDRNLVENLFSNGHIKVLVCTATLAWGVNLPAHAVVIKGT--------QIYAAKRGSFVDLGILDVMQIFGRAGRPQFDKFGEGIIITTHDK 873 HSP 4 Score: 53.1434 bits (126), Expect = 1.741e-25 Identity = 28/109 (25.69%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGS--ICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
+L ++LNAEIAL + +++ +W+ ++LY+R+ NP YG+ + I + + +L+I KL +I ++ T +T G + +++ + TI+ +L
Sbjct: 894 SLADNLNAEIALGTVTNVEEAVRWMSYTYLYVRMRANPLAYGISHKAYQIDPTLRKHREQLLIEVGQKLDKAKMIRFEERTGYFSSTDLGRTASHFYIKYNTIETFNEL 1002
BLAST of Helicase for meiosis 1 vs. UniProt/SwissProt
Match: sp|A2RUV5|HFM1_XENTR (Probable ATP-dependent DNA helicase HFM1 OS=Xenopus tropicalis OX=8364 GN=hfm1 PE=2 SV=1) HSP 1 Score: 198.749 bits (504), Expect = 3.024e-110 Identity = 100/295 (33.90%), Postives = 169/295 (57.29%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCS--LHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLN 1718
+S IS CSE S++ LR ER +N LNK + +R+P++G+I MK++CL+Q LGC+ V D SL Q+ KIFR G R+ +C+ ++L L + + ++ +L KCF +KLW+N+ +S Q+ +I +A + NAG +T +I ++ A+ +E I+N+ PPFG + + + P+YE+ +Q+ + + L V +T +Q + D F ++L+IGD +N +K +IM+ L + W +I + R S+ +C++LIS +VG DI+ F YL
Sbjct: 679 VSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQE---NKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAP-DSHF----VMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLG 965 HSP 2 Score: 150.984 bits (380), Expect = 3.024e-110 Identity = 72/151 (47.68%), Postives = 103/151 (68.21%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEFAHQFD 447
FC TRK Q A L +F + + +L + I + KLR + GVG+HHAG+ DR+++E+SFL G LPVL +TSTL+MGVNLPAHLV++K T ++ S + Q Y++ +LQMIGRAGRPQFD++A A+I+T S++E++ H D
Sbjct: 402 FCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLD 552 HSP 3 Score: 95.9005 bits (237), Expect = 3.024e-110 Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +L+EHLNAEIALH + D+ +WI+S+FLYIR +NPAYYG G I + +L + ++ LS+ LI D+ N + T G LMA Y++ F T + + G+ETL LV
Sbjct: 560 SLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEIN-FKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELV 679
BLAST of Helicase for meiosis 1 vs. UniProt/SwissProt
Match: sp|A2PYH4|HFM1_HUMAN (Probable ATP-dependent DNA helicase HFM1 OS=Homo sapiens OX=9606 GN=HFM1 PE=1 SV=2) HSP 1 Score: 183.726 bits (465), Expect = 8.014e-103 Identity = 103/294 (35.03%), Postives = 163/294 (55.44%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDS--MLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYL 1715
++ I+ C E +I LR E+ +N LNK +RFP++GRI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D+ +++ + L++ +L KCF KLW+N+ VS Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + P+YEL +EQI + + VT + + +Q + R +DS + LIIGD +N V Y +I + L+ + W +I V R + L I+LIS VG DI+ K YL
Sbjct: 810 VTLIAGCKEFLDIQLRINEKKTLNTLNKDPNRITIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTAKIFRHGSRITRWLSDF---VAAQEKKFAVLLNSLILAKCFRCKLWENSLHVSKQLEKIGITLSNAIVNAGLTSFKKIEETDARELELILNRHPPFGTQIKETVMYLPKYELKVEQITRYSD--TTAEILVTVILRNFEQLQTK---RTASDSHYVTLIIGDADNQVVYLHKITDSVLLKAGSWAKKIAVKRALKSEDLSINLISSEFVGLDIQQKLTVFYL 1095 HSP 2 Score: 139.813 bits (351), Expect = 8.014e-103 Identity = 66/138 (47.83%), Postives = 91/138 (65.94%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT 414
FC TRK Q A LV +F + +Q +L + + + KLR + G +HHAG+ DR++VE +F G LPVL +TSTL+MGVNLPAHLVVIK T ++ + + Y++ +LQMIGRAGRPQFD +A A+I+T
Sbjct: 533 FCATRKGVQQAASVLVKDAKFIMTVEQKQRLQKYAYSVRDSKLRDILKDGAAYHHAGMELSDRKVVEGAFTVGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYAGGLFEEYSETDILQMIGRAGRPQFDTTATAVIMT 670 HSP 3 Score: 97.8265 bits (242), Expect = 8.014e-103 Identity = 50/121 (41.32%), Postives = 77/121 (63.64%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ +WI+S+ LYIR +NP++YG +G + I + +L + ++ LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL+ LV
Sbjct: 691 SLHRHLIEHLNAEIVLHTITDVNIAVEWIRSTLLYIRALKNPSHYGFASGLNKDGIEAKLQELCLKNLNDLSSLDLIKMDEGVN-FKPTEAGRLMAWYYITFETVKKFYTISGKETLSDLV 810
BLAST of Helicase for meiosis 1 vs. UniProt/SwissProt
Match: sp|D3Z4R1|HFM1_MOUSE (Probable ATP-dependent DNA helicase HFM1 OS=Mus musculus OX=10090 GN=Hfm1 PE=3 SV=2) HSP 1 Score: 184.111 bits (466), Expect = 5.516e-102 Identity = 111/314 (35.35%), Postives = 167/314 (53.18%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHV-FVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNR--ISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTN 1772
IS IS C+E ++ LR +E+ +N LNK +RFP+ RI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D+ +++ + L++ +L KCF KLW+N+ VS Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + + P+YEL +EQI + V + R ++ D + LIIGD +N V +K +IM+ L+ S W +I V R ISED L I+LIS +VG DI KF Y P KF N
Sbjct: 808 ISMISSCNEFLDVQLRISEKRILNTLNKDPNRITIRFPMAERIKTREMKVNCLIQAQLGCIPIQDFALTQDTVKIFRNGSRIARWLSDF---VAAQEKKFAVLLNSVILTKCFKCKLWENSKHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPFGTQIKEAVAHLPKYELEVEQIARYSDIKAEILVTIILRNFEQLQTKRTAPDFHYAT----LIIGDADNQVVFKHKIMDSVLLKSGNWVKKIDVKRALISED--LSINLISSDYVGLDIHQKFTVFYF------------GPRKFVN 1100 HSP 2 Score: 140.198 bits (352), Expect = 5.516e-102 Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEF 432
FC TRK Q A LV +F + +Q +L + I + KL+ ++ GVG+HHAG+ DR++VE F G LPVL +TSTL+MG+N+PAHLVVIK T +++ + + Y++ +LQMIGRAGRPQFD +A A+I+T S+ E++
Sbjct: 531 FCSTRKGVQQAASVLVKDAKFIISVEQKLRLQKSAYSIRDSKLKDTLVYGVGYHHAGMELSDRKLVEGLFTSGDLPVLFTTSTLAMGMNMPAHLVVIKSTMHYSGGVFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKY 676 HSP 3 Score: 93.9745 bits (232), Expect = 5.516e-102 Identity = 49/121 (40.50%), Postives = 74/121 (61.16%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ WI+S+ LYIR +NP++YG +G + I + +L + + LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL L+
Sbjct: 689 SLHRHLIEHLNAEIVLHTITDVNIALDWIRSTMLYIRALKNPSHYGFSSGLNKDGIEAKLQELCLKNLKDLSSLDLIKMDEDVN-FKPTEAGRLMAWYYITFETVKKFCAISGKETLLDLI 808
BLAST of Helicase for meiosis 1 vs. UniProt/SwissProt
Match: sp|Q5D892|MER3_ARATH (DExH-box ATP-dependent RNA helicase DExH17 OS=Arabidopsis thaliana OX=3702 GN=MER3 PE=2 SV=1) HSP 1 Score: 119.398 bits (298), Expect = 3.542e-61 Identity = 64/151 (42.38%), Postives = 94/151 (62.25%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKF--------DQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSSE 423
FC TRK + A+ L QT + +Q+ +L S S++++++ I++GVG+H+ GLCQ DR +VE FL G + V+C+T+TL+ G+NLPAH VVIK T +FN Y ++ LLQM GRAGRP FD++ + II+T E
Sbjct: 280 FCSTRKGAQEAAQKLA-QTAMTYGYSNPFIKSREQLERLREASPMCSDKQMQSYILQGVGYHNGGLCQKDRSLVEGLFLNGDIQVICTTNTLAHGINLPAHTVVIKSTQHFNKEKGHYMEYDRSTLLQMSGRAGRPPFDDTGMVIIMTRRE 429 HSP 2 Score: 100.138 bits (248), Expect = 3.542e-61 Identity = 79/295 (26.78%), Postives = 140/295 (47.46%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPL-------KGRIMNASMKIDCLVQVIL-GCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGF-PRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIG-DLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCI--DLISEMHVGFDI 1688
+ + E+S I LR E+ +N++N + +LRF + K RI K+ L L G V D S+ Q+ + I G RI RC+ +Y NY+ +S TLL K KLWD++ ++ Q+P I V A L + G + +A + + IE + + PFG T+ + S P+ E+ +E++ ++ + L V ++R+ Q ++ R Y D LI+G + N +H+ +I S+ + +++ R + + + DLI E ++G D+
Sbjct: 567 LHIVCHAEEISWIQLRRNEKKTLNDVNADK-EGRLRFHINDNKGKRKKRIQTREEKLFVLANDWLTGDPSVHDLSMTQDANSICSNGSRIARCMKEYFIYKK----NYKGTLSSTLLAKSLYQKLWDDSPYLLKQLPGIGMVTAKALHSMGVRSFEALAEADPRRIEIVTGRKYPFGNTIKESLSSLPPKVEIKVEEVDCQKQGISKLAVTLSRVSQPLQSTK-----RHYAD---LIVGSEEENLIHFHEKIRMEDF--SSPYSVTVLLERPHQQTKVTVKADLIFEEYIGIDL 846 HSP 3 Score: 62.003 bits (149), Expect = 3.542e-61 Identity = 31/101 (30.69%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
LIEHL AEI + DI +W++ S+LY+R+ +NP Y + G + + + + +L + ++++LS +I D ++ G LM +Y+L F+T++
Sbjct: 452 LIEHLTAEIVQLTISDITRAIEWMKCSYLYVRMKKNPENYAIKKGIPKDRVEKHLQELCLQKINELSQYQMIWTDTDGFVLKPEEPGRLMTKYYLKFETMK 552
BLAST of Helicase for meiosis 1 vs. UniProt/SwissProt
Match: sp|B6DMK2|MER3_ORYSJ (ATP-dependent DNA helicase MER3 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=MER3 PE=2 SV=1) HSP 1 Score: 115.931 bits (289), Expect = 4.591e-60 Identity = 64/151 (42.38%), Postives = 90/151 (59.60%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFD--------QIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSN--IEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSE 423
FC TRK + A+ L QT L + Q L + S+++L+AC++ GVG+H+ GLC DR +VE FL G + +LC+T+TL+ G+NLPAH VVIK T FN + Y ++ +LQM GRAGRP FD++ II+T E
Sbjct: 287 FCSTRKGAQEAAQCL-SQTASSLGYSNPFMKSMQQYEHLKEAALTCSDKQLQACLVHGVGYHNGGLCLKDRSVVEGLFLKGDIQILCTTNTLAHGINLPAHTVVIKSTQFFNKEKGLYVEYERSMVLQMCGRAGRPPFDDTGTIIIMTRRE 436 HSP 2 Score: 98.5969 bits (244), Expect = 4.591e-60 Identity = 83/302 (27.48%), Postives = 141/302 (46.69%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHR---------GPNKLRFPLKGRIMNASMKI-----DCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGF-PRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVN-RISEDPTLCIDLISEMHVGFDIRTK 1697
+ I +E++ I LR E+ +N +N + G N R K RI KI DCL L V D SL+QE + I G R+ +C+ +Y NY++ +S LL KC KLW+++ F+ Q+P I V A L AG + +A + A+ IES+ + PFG ++ P+ ++ IE G ++ ++ V +TR+ Q S+ + Y D +++ + +N + + +I + S+ + ++ V + TL +D+I E +VG DI K
Sbjct: 574 LHIICHSAEITWIQLRRNEKKLLNEINADKEGRLWFHVVGANGKR---KKRIQTREEKIFILANDCLTGDPL----VHDLSLNQEMNSICSNGCRVAKCMREYFIYKK----NYKSAISSMLLAKCLHQKLWESSPFLLKQLPGIGIVTAKALKTAGIDSFESLATADARKIESVTGRNYPFGDSIKSYLPSLGPKIDINIEDAGNRQGK-STIIVTLTRLSQAVGSSKQN-----YAD--MVVGSEEDNAILFHEKIKTQEF--SSPYSVKLYVPCPPNARATLKVDVIFEEYVGLDIHKK 854 HSP 3 Score: 63.1586 bits (152), Expect = 4.591e-60 Identity = 37/115 (32.17%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 446 IEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
+EHLNAEI + DI +W++ S+LYIRI +NP +YG+ E + + + + + ++ +L LI D+ ++ G LM +++L F T++ I K S TL L+
Sbjct: 460 VEHLNAEIVQLTVSDITLAIEWLKCSYLYIRIKKNPQHYGIKKEIPRELLEKQMKDICVEKIHELGEYGLIWTDEDGFLLKPLEPGRLMTKFYLKFDTMKLIVKASACCTLEDLL 574
BLAST of Helicase for meiosis 1 vs. TrEMBL
Match: A0A5G3L853 (Probable ATP-dependent DNA helicase HFM1 OS=Xenopus tropicalis OX=8364 GN=hfm1 PE=4 SV=1) HSP 1 Score: 199.134 bits (505), Expect = 5.359e-108 Identity = 100/295 (33.90%), Postives = 169/295 (57.29%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCS--LHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLN 1718
+S IS CSE S++ LR ER +N LNK + +R+P++G+I MK++CL+Q LGC+ V D SL Q+ KIFR G R+ +C+ ++L L + + ++ +L KCF +KLW+N+ +S Q+ +I +A + NAG +T +I ++ A+ +E I+N+ PPFG + + + P+YE+ +Q+ + + L V +T +Q + D F ++L+IGD +N +K +IM+ L + W +I + R S+ +C++LIS +VG DI+ F YL
Sbjct: 745 VSLISSCSEFSDVQLRANERRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLTQDISKIFRHGTRLAKCLSEFLALQE---NKFSAFLNALILTKCFKSKLWENSSHISKQLEKIGVTLANAMVNAGLTTFKKIEDTNARELELIVNRHPPFGNQIKESVAHLPKYEIKFQQLAKYRATTAEVVLTVLLTNFKQLQKKRTAP-DSHF----VMLVIGDDDNQAIFKQKIMDSSLFKTGSWTRKIEIKRASKSTNVCLNLISSEYVGLDIQQSFNICYLG 1031 HSP 2 Score: 150.984 bits (380), Expect = 5.359e-108 Identity = 72/151 (47.68%), Postives = 103/151 (68.21%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEFAHQFD 447
FC TRK Q A L +F + + +L + I + KLR + GVG+HHAG+ DR+++E+SFL G LPVL +TSTL+MGVNLPAHLV++K T ++ S + Q Y++ +LQMIGRAGRPQFD++A A+I+T S++E++ H D
Sbjct: 468 FCSTRKGVQQAASILTKDAKFVMSIEHKQRLQKCANSIKDSKLRDVLQYGVGYHHAGVDISDRKVIENSFLIGDLPVLFTTSTLAMGVNLPAHLVIVKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYVHMLD 618 HSP 3 Score: 96.2857 bits (238), Expect = 5.359e-108 Identity = 52/121 (42.98%), Postives = 73/121 (60.33%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +L+EHLNAEIALH + D+ +WI+S+FLYIR +NPAYYG G I + +L + ++ LS+ LI D+ N + T G LMA Y++ F T + + G+ETL LV
Sbjct: 626 SLHKHLVEHLNAEIALHTITDVKVALEWIRSTFLYIRALKNPAYYGFSEGLDKIGIEAKLQELCLKNLNDLSSLGLIKMDEEIN-FKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELV 745
BLAST of Helicase for meiosis 1 vs. TrEMBL
Match: A0A1L8GMZ3 (Uncharacterized protein OS=Xenopus laevis OX=8355 GN=XELAEV_18023344mg PE=4 SV=1) HSP 1 Score: 201.06 bits (510), Expect = 4.617e-107 Identity = 109/336 (32.44%), Postives = 185/336 (55.06%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCS--LHVFVTRMRQKSDQSEVDCDIRFYNDS--MLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKI-SKQNPEKFTNSKLDNKLPSCPLDKNLTCDT 1832
+S IS C+E S++ LR E+ +N LNK + +R+P++G+I MK++CL+Q LGC+ V D SL Q+ KIFR G R+ +C+ ++L L + + ++ L KCF +KLW+N+ VS Q+ +I +A + NAG +T +I ++ A+ IE I+N+ PPFG + + + P+Y++T +Q+ + + L V +T Q + R DS ++L+IGD +N V +K +IM+ L+ + W +I + R S +C++L+S +VG DI+ F YL S + ++Q E F+ +K+ P + DT
Sbjct: 780 VSLISSCAEFSDVQLRANEKRVLNTLNKDKNRVTIRYPMEGKIKTKEMKVNCLIQAHLGCILVQDFSLSQDISKIFRHGTRLAKCLSEFLALQE---NKFSAFLNALFLTKCFKSKLWENSLHVSKQLEKIGVTLANAMVNAGLTTFKKIEDTNAREIELIVNRHPPFGNQIKESVANLPKYDITFQQLSKYRISTAEVVLTVLLTNFEQLQKK-------RTAPDSHYVMLVIGDDDNQVIFKQKIMDSSLLKTGSWTRKIEIKRASTSTDVCLNLVSSEYVGLDIQRNFNIYYLGSDSSNAGVKTQQKSETFS-----SKISKSPTKRKYNADT 1100 HSP 2 Score: 147.517 bits (371), Expect = 4.617e-107 Identity = 71/151 (47.02%), Postives = 102/151 (67.55%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEFAHQFD 447
FC TRK Q A L +F + + +L + I + KLR + GVG+HHAG+ DR+++E++FL G LPVL +TSTL+MGVNLPAHLV+IK T ++ S + Q Y++ +LQMIGRAGRPQFD++A A+I+T S++E++ D
Sbjct: 503 FCATRKGVQQAASILTKDAKFVMSIEHKQRLQKCANPIKDSKLRDVVQYGVGYHHAGVDIHDRKVIENTFLMGDLPVLFTTSTLAMGVNLPAHLVIIKSTMHYVSGMFQEYSETDILQMIGRAGRPQFDSTATAVIMTRLSTKEKYLQMLD 653 HSP 3 Score: 94.3597 bits (233), Expect = 4.617e-107 Identity = 51/121 (42.15%), Postives = 73/121 (60.33%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +L+EHLNAEIALH + D++ +WI+S+FLYIR +N AYYG G I + +L + ++ LS+ LI D+ N + T G LMA Y++ F T + + G+ETL LV
Sbjct: 661 SLHKHLVEHLNAEIALHTITDVNVALEWIRSTFLYIRALKNSAYYGFSEGLDKMGIEAKLQELCLKNLNDLSSVGLIKMDEEIN-FKPTETGKLMALYYIAFNTAKLFHTIRGTETLAELV 780
BLAST of Helicase for meiosis 1 vs. TrEMBL
Match: F7ARR8 (Helicase for meiosis 1 OS=Monodelphis domestica OX=13616 GN=HFM1 PE=4 SV=3) HSP 1 Score: 200.29 bits (508), Expect = 3.157e-106 Identity = 121/352 (34.38%), Postives = 189/352 (53.69%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYL--NYIESQTKISKQNPEKFTNSKLDNKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRN 1889
++ I+ C E +I LR E+ +N LNK + +RFP++GRI MK++CL+Q LGCL + D SL Q+T KIFR G RI + + +YL + + L++ +L KCF AKLW+N+ VS Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + + P+YEL +EQI + L V V + Q++ Y + LIIGD NN V +K ++M+ L+ + W +I V R + L I+LIS +VG DI+ KF Y+ N E+Q + ++N E T+S+ + P + T + TCKIS N
Sbjct: 631 VTMIAGCKEFLDIQLRINEKKTLNTLNKDQNRITIRFPMEGRIKTREMKVNCLIQAQLGCLPIQDFSLTQDTAKIFRSGTRITKWLSEYLAMQEK---KFAVLLNSLILAKCFRAKLWENSLHVSKQLEKIGVTLSNAMANAGLTSFKKIEDINARELELILNRHPPFGNQIKETVTHLPKYELEVEQISRYSNTGAELLVTVFLRNFEQLQTKRTAPDSHY---VTLIIGDANNQVVFKHKLMDSVLLKTGNWTKKIDVKRALQSEDLSINLISSEYVGLDIQQKFTAFYIGPNRFENQITM-QRNVETETHSRHSDGSPRKDVSAGSTSNRK----------NTCKISGN 965 HSP 2 Score: 141.739 bits (356), Expect = 3.157e-106 Identity = 70/146 (47.95%), Postives = 96/146 (65.75%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEF 432
FC TRK Q A L RF + Q +L + + + KLR + G+G+HHAG+ DR+IVE +F G LPVL +TSTL+MGVNLPAHLVVIK T ++ + + Y++ +LQMIGRAGRPQFD +A A+I+T S+ E++
Sbjct: 354 FCATRKGVQQAASVLSKNARFAMTMGQKQRLQKCTHSLRDSKLRDLLTCGIGYHHAGMELSDRKIVEEAFTVGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYVGGMFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKY 499 HSP 3 Score: 98.5969 bits (244), Expect = 3.157e-106 Identity = 50/121 (41.32%), Postives = 78/121 (64.46%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ +WI+S+ LYIR +NPA+YG +G I + +L + ++ LS+ LI D+ N ++T G LMA Y++ F+T++ ++G+ETL+ LV
Sbjct: 512 SLHRHLIEHLNAEIVLHTITDVNVALEWIRSTLLYIRALKNPAHYGFASGLDKNGIESKLQELCLKNLNDLSSVDLIKMDEDVN-FKSTEAGRLMAWYYITFETVKKFCIINGTETLSELV 631
BLAST of Helicase for meiosis 1 vs. TrEMBL
Match: G1L0V4 (Helicase for meiosis 1 OS=Ailuropoda melanoleuca OX=9646 GN=HFM1 PE=4 SV=1) HSP 1 Score: 196.823 bits (499), Expect = 4.249e-105 Identity = 111/335 (33.13%), Postives = 183/335 (54.63%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLN--YIESQTKISKQNPEKFTNSKLDNKLPSCPLDKNLT-CDTP 1835
++ I+ C E ++ LR E+ +N LNK +RFP++GRI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D++ + + L++ +L KCF KLW+N+ +S Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + P+YEL +EQI M + V + + Q++ Y + LIIGD +N + +K RIM+ L+ + W +I V R + LCI+LIS +VG DI+ KF YL + +Q + K++ + ++SK N+ +K +T C P
Sbjct: 813 VTMIASCKEFVDVQLRINEKKTLNTLNKDPNRVTIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTAKIFRNGSRITRWLSDFVAVQEK---KFAVLLNSLILAKCFRCKLWENSLHISKQLEKIGITLSNAMVNAGLTSFKKIEETDARELELILNRHPPFGTQIKETVMYLPKYELEVEQIARYSDVMAEILVTIILRNFEQLQTKRTAPDSHY---VTLIIGDADNQLVFKHRIMDSVLLKAGNWTKKIDVKRALKSEDLCINLISSEYVGLDIQRKFTVFYLGPKRLGNQIIMQKKSETEISHSKHSNRSTIAGPNKGMTACKKP 1141 HSP 2 Score: 144.821 bits (364), Expect = 4.249e-105 Identity = 71/146 (48.63%), Postives = 98/146 (67.12%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEF 432
FC TRK Q A LV +F + +Q +L + I + KLR +I GV +HHAG+ DR++VE +F G LPVL +TSTL+MGVNLPAHLVVIK T ++ + + Y++ +LQMIGRAGRPQFD +A A+I+T S++E +
Sbjct: 536 FCATRKGVQQAASVLVKDAKFIMAVEQKQRLQKCAYSIRDSKLRDILIHGVAYHHAGMELSDRKVVEGAFTVGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYAGGMFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTKERY 681 HSP 3 Score: 95.5153 bits (236), Expect = 4.249e-105 Identity = 50/121 (41.32%), Postives = 76/121 (62.81%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ +WI+S+ LYIR +NP++YG +G + I + +L + ++ LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL LV
Sbjct: 694 SLHRHLIEHLNAEIVLHTITDVNIALEWIRSTLLYIRALKNPSHYGFASGLNKDGIEAKLQELCLKNLNDLSSLDLIRMDEDIN-FKPTEAGRLMAWYYITFETVKKFCTISGKETLLDLV 813
BLAST of Helicase for meiosis 1 vs. TrEMBL
Match: A0A3Q7VWX0 (probable ATP-dependent DNA helicase HFM1 OS=Ursus arctos horribilis OX=116960 GN=HFM1 PE=4 SV=1) HSP 1 Score: 196.438 bits (498), Expect = 4.284e-105 Identity = 107/319 (33.54%), Postives = 177/319 (55.49%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLN--YIESQTKISKQNPEKFTNSKLDNK 1790
++ I+ C E ++ LR E+ +N LNK +RFP++GRI MK++CL+Q LGC+ + D +L Q+T KIFR G RI R + D++ + + L++ +L KCF KLW+N+ +S Q+ +I ++ + NAG ++ +I + A+ +E ILN+ PPFG + + P+YEL +EQI M + V + + Q++ Y + LIIGD +N + +K RIM+ L+ + W +I V R + LCI+LIS +VG DI+ KF YL + +Q + K++ + ++SK N+
Sbjct: 813 VTMIASCKEFVDVQLRINEKKTLNTLNKDPNRVTIRFPMEGRIKTREMKVNCLIQAQLGCIPIQDFALTQDTAKIFRNGSRITRWLSDFVAVQEK---KFAVLLNSLILAKCFRCKLWENSLHISKQLEKIGITLSNAMVNAGLTSFKKIEETDARELELILNRHPPFGTQIKETVMYLPKYELEVEQIARYSDTMAEILVTIILRNFEQLQTKRTAPDSHY---VTLIIGDADNQLVFKHRIMDSVLLKAGNWTKKIDVKRAFKSEDLCINLISSEYVGLDIQQKFTVFYLGPKRLGNQIIMQKKSETEISHSKHSNR 1125 HSP 2 Score: 145.206 bits (365), Expect = 4.284e-105 Identity = 71/146 (48.63%), Postives = 99/146 (67.81%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT--SSEEEF 432
FC TRK Q A LV +F + +Q +L + I + KLR +I GV +HHAG+ DR++VE +F G LPVL +TSTL+MGVNLPAHLVVIK T ++ + + Y++ +LQMIGRAGRPQFD +A A+I+T S++E++
Sbjct: 536 FCATRKGVQQAASVLVKDAKFIMAVEQKQRLQKCAYSIRDSKLRDILIHGVAYHHAGMELSDRKVVEGAFTVGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYAGGMFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTKEKY 681 HSP 3 Score: 95.1301 bits (235), Expect = 4.284e-105 Identity = 50/121 (41.32%), Postives = 76/121 (62.81%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D++ +WI+S+ LYIR +NP++YG +G + I + +L + ++ LS+ LI D+ N + T G LMA Y++ F+T++ +SG ETL LV
Sbjct: 694 SLHRHLIEHLNAEIVLHTITDVNIALEWIRSTLLYIRALKNPSHYGFASGLNKDGIEAKLQELCLKNLNDLSSLDLIRMDEDIN-FKPTEAGRLMAWYYITFETVKKFCTISGKETLLDLV 813
BLAST of Helicase for meiosis 1 vs. Ensembl Cavefish
Match: hfm1 (helicase for meiosis 1 [Source:ZFIN;Acc:ZDB-GENE-061207-43]) HSP 1 Score: 176.022 bits (445), Expect = 3.844e-102 Identity = 95/296 (32.09%), Postives = 168/296 (56.76%), Query Frame = 3
Query: 825 NICY*ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNY 1712
N+ I +S+ E S++ LR E+ +N LN + +R+P++G+I + MK++CL+Q LG L + + L Q+T KIFR G+RI +C+ ++L S N+ ++ +L KCF AKLW+++ +VS Q+ +I +A + NAG ++ ++I + + +E I+N+ PPFG + + S P+YE+ +EQ+ + + + V V + ++ Y + LIIGD +N+V ++ +I + L+ S W +I V R S++ + I+LIS +VG DI+ F Y
Sbjct: 765 NLAELIEMMSKGKEFSDVQLRVNEKRTLNTLNIDKNRVTIRYPMEGKIKSKDMKVNCLIQAQLGNLPIQEFGLTQDTGKIFRSGVRISKCLAEFLSHHSK--QNFSAQLNSLILAKCFRAKLWEDSPYVSKQLDKIGLSLATAMVNAGLTSFSKIEATNPRELELIVNRHPPFGNQIKEAVSKLPKYEIRLEQLPKYSVSTAEILVTVNLKNFEELLTKRTAPDHHY---VTLIIGDCDNNVVFQQKIQDTVLLKSGSWSRKIEVTRTSQEDKISINLISSEYVGLDIQQTFTAFY 1055 HSP 2 Score: 142.51 bits (358), Expect = 3.844e-102 Identity = 71/138 (51.45%), Postives = 91/138 (65.94%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT 414
FC TRK Q A L RF + D +L + I + KLR I+ GVG+HHAG+ DR++VE +F G LPVL +TSTL+MGVNLPAHLVVIK T ++ + Y++ +LQMIGRAGRPQFD SA A+I+T
Sbjct: 493 FCSTRKGVQQSATVLAKDARFIMSIDHRQRLMKYASSILDAKLRDLIMCGVGYHHAGVSLSDRKVVEDAFTAGDLPVLFTTSTLAMGVNLPAHLVVIKSTMHYAGGSCEEYSEADMLQMIGRAGRPQFDTSATAVIMT 630 HSP 3 Score: 97.8265 bits (242), Expect = 3.844e-102 Identity = 50/121 (41.32%), Postives = 74/121 (61.16%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH NL+EHLNAEI LH + D++ WI+S+FLYIR +NP +YG + I + +L + ++ L++ LI D+ N I+ T G LMARY + F T++ KL+G+E L L+
Sbjct: 651 SLHTNLVEHLNAEIVLHTISDVNMALDWIRSTFLYIRALKNPTHYGFSSELDKCGIETKLQELCLKNLNSLASYQLITMDEDIN-IKPTESGKLMARYCVAFDTMKQFSKLTGTENLAELI 770
BLAST of Helicase for meiosis 1 vs. Ensembl Cavefish
Match: snrnp200 (small nuclear ribonucleoprotein U5 subunit 200 [Source:NCBI gene;Acc:103030392]) HSP 1 Score: 88.9669 bits (219), Expect = 1.106e-28 Identity = 45/104 (43.27%), Postives = 66/104 (63.46%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQ---NKLLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT ++ + +T+ +LQM+GRAGRPQ+D I++TS E
Sbjct: 770 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPE-KGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 872 HSP 2 Score: 49.2914 bits (116), Expect = 1.106e-28 Identity = 27/108 (25.00%), Postives = 53/108 (49.07%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAE+ L + ++ WI ++LY+R+ RNP Y V S + Q M L+ + L +L+ +D+ + + T G + + +++ ++I +L
Sbjct: 894 LPDMLNAEVVLGNVQNVKDAVNWIGYTYLYVRMLRNPTLYAVSHDERSTDPYLEQRRMDLVHTAATVLEKNNLVKYDKRSGSFQVTDLGRIASHFYITHESIMTYNQL 1001 HSP 3 Score: 30.4166 bits (67), Expect = 1.106e-28 Identity = 35/184 (19.02%), Postives = 80/184 (43.48%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE I +R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +T+ C ++ K ++W + + ++PE + ++ + F L + +++ L + P G+T+ K FP+ +L +
Sbjct: 1016 SLSSEFRNITVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAYISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLGRGWAQLTDKTMNLCKMIDK----RMWQSMSPLRQFRKLPE-EVIKKIEKKSFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHQFPKLDLAV 1185 HSP 4 Score: 73.9442 bits (180), Expect = 1.803e-17 Identity = 41/116 (35.34%), Postives = 65/116 (56.03%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEEFAHQF 444
+K+S+ LR + GVG+ H GL + +IVE F GA+ V+ ++ +L G N+ AHLV++ T +N I Y +LQM+G+A RP Q D I+ S+++F +F
Sbjct: 1599 EKLSDATLRETLSNGVGYLHEGLSSTECKIVEQLFNSGAIQVVVASRSLCWGTNISAHLVIVMDTQYYNGKIHAYVDYPIYDVLQMVGKANRPLQDDEGRCVIMCQGSKKDFFKKF 1714 HSP 5 Score: 36.965 bits (84), Expect = 1.803e-17 Identity = 24/103 (23.30%), Postives = 52/103 (50.49%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + + L I+ + ++++ P G++ A Y++++ TI+
Sbjct: 1729 LHDHFNAEIVTKTVENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGMSHRHLSDHLSELVENTLQDLEQSKCIS---VEDEMDVAPLNLGMIAAYYYINYTTIE 1827
BLAST of Helicase for meiosis 1 vs. Ensembl Cavefish
Match: ascc3 (activating signal cointegrator 1 complex subunit 3 [Source:NCBI gene;Acc:100332599]) HSP 1 Score: 90.1225 bits (222), Expect = 2.336e-17 Identity = 56/152 (36.84%), Postives = 81/152 (53.29%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVD--QTRFELKFDQIHQLGVIS------QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
F R ST + A L++ + R E F Q+ Q Q+ ++LR G G HHAG+ + DR ++E+ F G L VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQFD I+T+ ++
Sbjct: 721 FVHARNSTVRTAMSLIEMAKNRGESSFFQVDQGPDYGHCEKQVQRSRNKQLREMFPDGFGIHHAGMLRQDRTLMENMFSRGYLKVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGAMVDLGILDVMQIFGRAGRPQFDKYGEGTIITTHDK 872 HSP 2 Score: 72.4034 bits (176), Expect = 5.907e-17 Identity = 39/103 (37.86%), Postives = 59/103 (57.28%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFN 312
TR + L FL D + L D+ +I+ + + L+ + G+G HHAGL + DR+ VE F+ + VL +TSTL+ GVN PAHLV++KGT ++
Sbjct: 1528 TRLTALDLIAFLATEDDPKQWLHQDEREMTDIIA-TVRDSNLKLTLAFGIGMHHAGLHERDRKTVEELFVNCKIQVLIATSTLAWGVNFPAHLVIVKGTEYYD 1629 HSP 3 Score: 36.965 bits (84), Expect = 5.907e-17 Identity = 34/148 (22.97%), Postives = 63/148 (42.57%), Query Frame = 2
Query: 302 QILTLILSKHILKINYYK*SVGRVGRNLTTVPWP*F*LVARRSLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
++L ++ H +K ++YK P+P SL L +HLNAEIA + +I ++ + R+ NP+YY + + E I + + L+ + L + + IE G + + Y+L QT++
Sbjct: 1644 EVLAGLMLLHDIKKDFYK--------KFLYEPFP-----VESSLLGVLSDHLNAEIAAGTVTSKQDAMDYITWTYFFRRLVMNPSYYNLDDIN-HETINKFLSNLVEKSLRDLECSYCMEIQEDERTIEPLTYGRIASYYYLKHQTVR 1777
BLAST of Helicase for meiosis 1 vs. Ensembl Cavefish
Match: polq (DNA polymerase theta [Source:NCBI gene;Acc:103039702]) HSP 1 Score: 72.0182 bits (175), Expect = 7.217e-12 Identity = 42/100 (42.00%), Postives = 59/100 (59.00%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEE 426
L+ + GV FHHAGL +R+I+E +F G + VL +TSTLS GVNLPA V+I+ T FN ++ T QM+GRAGR D ++++ E
Sbjct: 578 LQRTVPWGVAFHHAGLTFDERDILEGAFRQGFIRVLAATSTLSSGVNLPARRVIIR-TPVFNGHLLDILTYK---QMVGRAGRKGVDTIGESVLVCKEAE 673
BLAST of Helicase for meiosis 1 vs. Ensembl Cavefish
Match: skiv2l (Ski2 like RNA helicase [Source:NCBI gene;Acc:103028968]) HSP 1 Score: 69.3218 bits (168), Expect = 3.862e-11 Identity = 35/98 (35.71%), Postives = 53/98 (54.08%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSS 420
+R + RG+G HH+G+ + +E++E F G + VL +T T +MGVN+PA VV + + + +QM GRAGR D + IIL S
Sbjct: 633 MRDLLKRGIGVHHSGILPILKEVIEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSIRKHDGTGFRNLLPGEYIQMAGRAGRRGLDATGTVIILCKS 730
BLAST of Helicase for meiosis 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003190.1 (pep scaffold:Pmarinus_7.0:GL480029:355:29241:-1 gene:ENSPMAG00000002910.1 transcript:ENSPMAT00000003190.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 117.857 bits (294), Expect = 3.703e-73 Identity = 62/190 (32.63%), Postives = 107/190 (56.32%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSD-CDNYQTLVSCTLLKKCFVAKLWDNT-WFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQ 1403
++ ++ C E I LR E+ +N N + + +RFP+ G+I MK++CL+Q LGC+ + D +L Q+ KIFR G+R+ +C+ + FL D +N+Q +++ L KCF AKLW N+ F + ++ ++ L NAG ++ +I A I+N+ PPFG + + P+Y++ +EQ
Sbjct: 396 VNLVAHCREYKYIALRVNEKKTLNTFNMAKN-HTIRFPMPGKIKTIEMKVNCLIQPQLGCV-LQDFALIQDIAKIFRTGIRLTKCLAE--FLQQKDKQNNFQAVLNAVHLAKCFRAKLWKNSPVFKQQNVSAVSFSLSSALVNAGLTSFEKIYTVNAIGPVVIVNRHPPFGNQIKEAVQHLPKYQVRVEQ 581 HSP 2 Score: 114.005 bits (284), Expect = 3.703e-73 Identity = 68/147 (46.26%), Postives = 89/147 (60.54%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFD--QIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFN-SNIEQTYTQNKLLQMIGRAGRP-QFDNSAVAIILTSSEEE 429
FC TR+S Q A L +F + F Q H S + + KLR +++G+ +HHAG+ DR +E FL G L VL STSTL++GVNLPAHLV++K T + + Q Y + LQMIGRAGRP QFD SA A+ILT + E
Sbjct: 122 FCATRRSVQQAAATLAKYAKFMIDFQHRQSHCRKHASH-LKDIKLRDLLMKGIAYHHAGMAASDRRNIEDVFLQGELRVLFSTSTLAIGVNLPAHLVIVKSTMRYTRGGMLQEYGET--LQMIGRAGRPQQFDVSATAVILTRKKYE 265 HSP 3 Score: 84.7297 bits (208), Expect = 3.703e-73 Identity = 50/130 (38.46%), Postives = 69/130 (53.08%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIM---------KLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLHINL EHLNAEI LH + D++ WI+S+FLYIR +NP +Y P G E I + ++ L +++ + NG I+ T G LMA Y++ F TI+ L GSE + LV
Sbjct: 277 SLHINLTEHLNAEIVLHTITDVNVALDWIRSTFLYIRALKNPKHYKFPAGLSKEDIEKKLLSELCLESLNSLATFKLNCMQNGD----------IKPTDVGRLMASYYIAFDTIKLFGTLQGSEAMQDLV 396
BLAST of Helicase for meiosis 1 vs. Ensembl Sea Lamprey
Match: ascc3 (activating signal cointegrator 1 complex subunit 3 [Source:NCBI gene;Acc:100332599]) HSP 1 Score: 104.375 bits (259), Expect = 1.654e-22 Identity = 59/150 (39.33%), Postives = 85/150 (56.67%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L FL D + L D+ +IS + + L+ C+ G+G HHAGL + DR VE F+ + VL +TSTL+ GVN PAHLV+IKGT F+ + + +LQM+GRAGRPQFD+ A+I +++F +F
Sbjct: 939 TRLTALDLIAFLAGEDNPKQWLHTDETEMEHIIS-TVKDSNLKLCLAFGIGMHHAGLHERDRRTVEELFVNTRIQVLIATSTLAWGVNFPAHLVIIKGTEYFDGKTRRYVDFPITDVLQMMGRAGRPQFDDQGKAVIFVHDLKKDFYKKF 1087 HSP 2 Score: 81.6481 bits (200), Expect = 5.944e-22 Identity = 43/109 (39.45%), Postives = 64/109 (58.72%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
++ + +R G HHAG+ + DR +VE F G + VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQ+D + II+TS ++
Sbjct: 130 ERSRNKPMRELFPDGFSTHHAGMLRPDRSLVEGLFSRGLVRVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGTFVDLGALDVMQIFGRAGRPQYDTFGLGIIITSHDK 238 HSP 3 Score: 41.9726 bits (97), Expect = 5.944e-22 Identity = 28/129 (21.71%), Postives = 55/129 (42.64%), Query Frame = 2
Query: 431 LHINLIEHLNAE--IALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEA--IPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSG----SETLTSLVC 793
+L ++LNAE L I +W+ ++LY+R+ NP YG+ + + + ++++ KL +I D+ T T G + +++ + TI+ G + S+VC
Sbjct: 256 FQASLQDNLNAEDEAGLATAAYIFEAVRWLSYTYLYVRMRANPLAYGINYNELWRSPNVEWFREQMVVDAGRKLDKARMIRFDERTGYFSPTDTGRTASHFYIKYNTIETFNDYFGPHLTESDVLSMVC 384
BLAST of Helicase for meiosis 1 vs. Ensembl Sea Lamprey
Match: ascc3 (activating signal cointegrator 1 complex subunit 3 [Source:NCBI gene;Acc:100332599]) HSP 1 Score: 104.375 bits (259), Expect = 1.655e-22 Identity = 59/150 (39.33%), Postives = 85/150 (56.67%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L FL D + L D+ +IS + + L+ C+ G+G HHAGL + DR VE F+ + VL +TSTL+ GVN PAHLV+IKGT F+ + + +LQM+GRAGRPQFD+ A+I +++F +F
Sbjct: 940 TRLTALDLIAFLAGEDNPKQWLHTDETEMEHIIS-TVKDSNLKLCLAFGIGMHHAGLHERDRRTVEELFVNTRIQVLIATSTLAWGVNFPAHLVIIKGTEYFDGKTRRYVDFPITDVLQMMGRAGRPQFDDQGKAVIFVHDLKKDFYKKF 1088 HSP 2 Score: 81.6481 bits (200), Expect = 7.179e-22 Identity = 43/109 (39.45%), Postives = 64/109 (58.72%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
++ + +R G HHAG+ + DR +VE F G + VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQ+D + II+TS ++
Sbjct: 131 ERSRNKPMRELFPDGFSTHHAGMLRPDRSLVEGLFSRGLVRVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGTFVDLGALDVMQIFGRAGRPQYDTFGLGIIITSHDK 239 HSP 3 Score: 41.9726 bits (97), Expect = 7.179e-22 Identity = 29/125 (23.20%), Postives = 55/125 (44.00%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIP---QIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSG----SETLTSLVC 793
+L ++LNAE +W+ ++LY+R+ NP YG+ S +A P + ++++ KL +I D+ T T G + +++ + TI+ G + S+VC
Sbjct: 260 SLQDNLNAEDEAGLATAAFEAVRWLSYTYLYVRMRANPLAYGI-NHSHYQADPSLHKFREQMVVDAGRKLDKARMIRFDERTGYFSPTDTGRTASHFYIKYNTIETFNDYFGPHLTESDVLSMVC 383
BLAST of Helicase for meiosis 1 vs. Ensembl Sea Lamprey
Match: helq (helicase, POLQ like [Source:ZFIN;Acc:ZDB-GENE-060503-421]) HSP 1 Score: 73.1738 bits (178), Expect = 3.734e-13 Identity = 44/150 (29.33%), Postives = 86/150 (57.33%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFL---VDQTRFELKFDQIHQLGVISQKISEEKL----RACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEEE 429
FC T+K+ +A+ + + +TR L + + ++++ ++ +L R + G+ +HH+GL +R ++E ++ G L +L TSTL+ GVNLPA V+++ NS + ++++ QM+GRAGR D+S +I + +S ++
Sbjct: 265 FCPTKKNCENVAEMICKYLAKTRHTLHHREEEKRALLAELQADGELCPVLRRTVPYGLAYHHSGLTGEERRLIEEAYSAGTLCLLTCTSTLAAGVNLPARRVILRSPYIGNSFL----SRSQYKQMVGRAGRAGIDSSGESITIVNSRDK 410
BLAST of Helicase for meiosis 1 vs. Ensembl Sea Lamprey
Match: helq (helicase, POLQ like [Source:ZFIN;Acc:ZDB-GENE-060503-421]) HSP 1 Score: 71.633 bits (174), Expect = 1.430e-12 Identity = 36/101 (35.64%), Postives = 62/101 (61.39%), Query Frame = 1
Query: 127 LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEEE 429
LR + G+ +HH+GL +R ++E ++ G L +L TSTL+ GVNLPA V+++ NS + ++++ QM+GRAGR D+S +I + +S ++
Sbjct: 385 LRRTVPYGLAYHHSGLTGEERRLIEEAYSAGTLCLLTCTSTLAAGVNLPARRVILRSPYIGNSFL----SRSQYKQMVGRAGRAGIDSSGESITIVNSRDK 481
BLAST of Helicase for meiosis 1 vs. Ensembl Yeast
Match: HFM1 (Meiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity [Source:SGD;Acc:S000003220]) HSP 1 Score: 130.183 bits (326), Expect = 3.078e-41 Identity = 66/141 (46.81%), Postives = 92/141 (65.25%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSE 423
FC TR ST AKFL++ F + + S++ L C+ +G+ FHHAG+ DR VE FL G++ +LCSTSTL++GVNLPA+LV+IKGT ++NS+ Q Y+ +LQMIGRAGRPQF+ A+I+T S+
Sbjct: 377 FCPTRASTISTAKFLLNNHIFSKSKKRCNH------NPSDKILNECMQQGIAFHHAGISLEDRTAVEKEFLAGSINILCSTSTLAVGVNLPAYLVIIKGTKSWNSSEIQEYSDLDVLQMIGRAGRPQFETHGCAVIMTDSK 511 HSP 2 Score: 57.7658 bits (138), Expect = 3.078e-41 Identity = 33/120 (27.50%), Postives = 61/120 (50.83%), Query Frame = 2
Query: 416 VARRSLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYY----------GVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQ 745
V SLH+NLIEHL AE +L + I++ W++++F Y+R +NPA Y V I + ++ L+ ++ +SNG + ++T G M R+++ F++++
Sbjct: 525 VLESSLHLNLIEHLAAETSLETVYSIETAVNWLRNTFFYVRFGKNPAAYQEVNRYVSFHSVEDSQINQFCQYLLDTLVKVKIIDISNG----------EYKSTAYGNAMTRHYISFESMK 634
BLAST of Helicase for meiosis 1 vs. Ensembl Yeast
Match: SLH1 (Putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses [Source:SGD;Acc:S000003503]) HSP 1 Score: 103.99 bits (258), Expect = 1.441e-28 Identity = 55/135 (40.74%), Postives = 79/135 (58.52%), Query Frame = 1
Query: 49 DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIE--QTYTQNKLLQMIGRAGRPQFDNSAVAIILTS-SEEEFAHQF 444
D R L D +L +++++ L+ + G+G HHAGL Q DR I F + +L +TSTL+ GVNLPAHLV+IKGT F++ IE + +LQM+GRAGRP +D + AI+ T S++ F F
Sbjct: 1397 DNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHAGLVQKDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMDLTDILQMMGRAGRPAYDTTGTAIVYTKESKKMFYKHF 1531 HSP 2 Score: 41.2022 bits (95), Expect = 1.441e-28 Identity = 31/112 (27.68%), Postives = 53/112 (47.32%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKL--SNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRK 757
SLH L +HL AEI + + ++ +FL+ R NP YYG+ + + + + LI S ++ L S L++ D I TP + + Y++ TI+ + K
Sbjct: 1541 SLHKVLDDHLGAEITSGSITNKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLENLRESQCVLLHGDDIV----ATPFLSISSYYYISHLTIRQLLK 1648 HSP 3 Score: 68.5514 bits (166), Expect = 1.078e-11 Identity = 30/56 (53.57%), Postives = 39/56 (69.64%), Query Frame = 1
Query: 148 GVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNS 315
G G HHAG+ + DR + E F GA+ VLC T+TL+ GVNLPA V+IKGT ++S
Sbjct: 594 GFGIHHAGMARSDRNLTEKMFKDGAIKVLCCTATLAWGVNLPADCVIIKGTQVYDS 649
BLAST of Helicase for meiosis 1 vs. Ensembl Yeast
Match: BRR2 (RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD [Source:SGD;Acc:S000000974]) HSP 1 Score: 90.8929 bits (224), Expect = 1.692e-24 Identity = 59/151 (39.07%), Postives = 84/151 (55.63%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQ----------TRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFN---SNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT 414
F +RK T + A +L ++ T+ + QI L + + + LR I G+G HHAGL + DR + E F G L VL T+TL+ GVNLPAH V+IKGT ++ + EQ Q+ +LQM+GRAGRP++D II+T
Sbjct: 743 FVHSRKETSRTATWLKNKFAEENITHKLTKNDAGSKQI--LKTEAANVLDPSLRKLIESGIGTHHAGLTRSDRSLSEDLFADGLLQVLVCTATLAWGVNLPAHTVIIKGTDVYSPEKGSWEQLSPQD-VLQMLGRAGRPRYDTFGEGIIIT 890 HSP 2 Score: 40.817 bits (94), Expect = 1.692e-24 Identity = 23/100 (23.00%), Postives = 50/100 (50.00%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTI 742
L+++LNAE+ + + W+ ++LY+R+ +P Y VP S + + L+ S + L L+ +D + IE T G + + ++++ ++
Sbjct: 916 LVDNLNAEVVAGNIKCRNDAVNWLAYTYLYVRMLASPMLYKVPDISSDGQLKKFRESLVHSALCILKEQELVLYDAENDVIEATDLGNIASSFYINHASM 1015 HSP 3 Score: 41.5874 bits (96), Expect = 1.883e-9 Identity = 37/128 (28.91%), Postives = 54/128 (42.19%), Query Frame = 2
Query: 455 LNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLI---------------NHDQITNKIETTPCGLLMARYFLDFQTIQD-IRKLSGSETLTSLV 790
LN EIA + W S+ Y RI NP+YYGV S I + L+ + ++ L S I D+ T I T GL+ + Y + F TIQ + LS + TL +++
Sbjct: 1754 LNNEIANSIIQSKQDCVDWFTYSYFYRRIHVNPSYYGVRDTS-PHGISVFLSNLVETCLNDLVESSFIEIDDTEAEVTAEVNGGDDEATEIISTLSNGLIASHYGVSFFTIQSFVSSLSNTSTLKNML 1880 HSP 4 Score: 38.891 bits (89), Expect = 1.883e-9 Identity = 40/148 (27.03%), Postives = 74/148 (50.00%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIH----QLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVA---IILTS 417
F +RK ++A + ++ +++D ++ Q+ +K+++ LRA + GVG + G+ D IV+ + GA+ VL + S V+I GT+ ++ + YT N+LL+M+G A N ++A +ILTS
Sbjct: 1584 FLPSRKDCMEVASAFMKFSK-AIEWDMLNVEEEQIVPYIEKLTDGHLRAPLKHGVGILYKGMASNDERIVKRLYEYGAVSVLLISKDCS-AFACKTDEVIILGTNLYDGAEHKYMPYTINELLEMVGLAS----GNDSMAGKVLILTS 1725
BLAST of Helicase for meiosis 1 vs. Ensembl Yeast
Match: SKI2 (Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome [Source:SGD;Acc:S000004390]) HSP 1 Score: 68.5514 bits (166), Expect = 7.752e-12 Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL 411
K R+ + RG+ HH GL + +E++E F G + VL +T T +MG+NLP V+ + N + T + QM GRAGR D++ I++
Sbjct: 683 KTRSLLERGIAVHHGGLLPIVKELIEILFSKGFIKVLFATETFAMGLNLPTRTVIFSSIRKHDGNGLRELTPGEFTQMAGRAGRRGLDSTGTVIVM 778
BLAST of Helicase for meiosis 1 vs. Ensembl Yeast
Match: MTR4 (ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing [Source:SGD;Acc:S000003586]) HSP 1 Score: 68.1662 bits (165), Expect = 1.126e-11 Identity = 33/95 (34.74%), Postives = 54/95 (56.84%), Query Frame = 1
Query: 145 RGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEEE 429
RG+G HH+GL + +E++E F G L VL +T T S+G+N+PA VV ++ + + + +QM GRAGR D+ + I++ + E
Sbjct: 470 RGIGIHHSGLLPILKEVIEILFQEGFLKVLFATETFSIGLNMPAKTVVFTSVRKWDGQQFRWVSGGEYIQMSGRAGRRGLDDRGIVIMMIDEKME 564
BLAST of Helicase for meiosis 1 vs. Ensembl Nematostella
Match: EDO34884 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN39]) HSP 1 Score: 156.377 bits (394), Expect = 1.216e-55 Identity = 68/189 (35.98%), Postives = 116/189 (61.38%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQI 1406
+ +S+C E +++ LR +E+ +N LNK + +RFP+ G+I KI+CL+Q LGCL + + SL+Q+ KIFR G R+ RC+ + L L +Y+ L + L+ KC ++LW+N+ +VS Q+ ++ ++ L NAG +T +I + + IE I+N+ PPFG + FP+YE+++EQ+
Sbjct: 277 VILLSKCKEFADVKLRVSEKRILNTLNKDKHKATVRFPVNGKIKTTDQKINCLIQATLGCLPITEFSLNQDVTKIFRSGQRVTRCLMELLMLRG----DYRALQNAILVTKCMKSRLWENSLYVSRQLEKVGMTLSTMLVNAGLTTFKKIEETNPREIELIVNRHPPFGSQIRDAVLAFPKYEVSVEQV 461 HSP 2 Score: 80.4925 bits (197), Expect = 1.216e-55 Identity = 43/121 (35.54%), Postives = 69/121 (57.02%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +LIEHLNAEI LH + D+ +W++S+FL+IRI +NP YY + I ++ L +L+ + + + P G LMARY++ F +I++ + G+ETL+ +V
Sbjct: 174 SLHRHLIEHLNAEIVLHTITDVSVALEWLKSTFLFIRIQKNPTYYD----------------MCIQNLNTLERSNLVKMEDGFDLKPSEP-GCLMARYYIAFDSIKEFLAIQGTETLSDMV 277 HSP 3 Score: 21.557 bits (44), Expect = 1.216e-55 Identity = 8/16 (50.00%), Postives = 13/16 (81.25%), Query Frame = 1
Query: 382 FDNSAVAIILTSSEEE 429
FD SA A+ILT++ ++
Sbjct: 143 FDTSATAVILTTTTKK 158
BLAST of Helicase for meiosis 1 vs. Ensembl Nematostella
Match: EDO49146 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH22]) HSP 1 Score: 90.1225 bits (222), Expect = 4.591e-28 Identity = 47/118 (39.83%), Postives = 68/118 (57.63%), Query Frame = 1
Query: 103 SQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
++++ +L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D I++TS E
Sbjct: 766 AEQVKNGELKDLLPYGFAIHHAGMSRVDRTLVEDLFADRHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWVELGALDILQMLGRAGRPQYDTKGEGILITSHGE 875 HSP 2 Score: 54.6842 bits (130), Expect = 4.591e-28 Identity = 32/109 (29.36%), Postives = 54/109 (49.54%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMK---LIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L ++LNAE+ L + W+ ++LYIR+ RNPA YG+ I E P + + LI S L +L+ +D+ + + T G + + Y+L +T+ L
Sbjct: 897 LADNLNAEVVLGSVQTAKDAVHWLGYTYLYIRMLRNPALYGISHDEI-EKDPLLEQRRADLIHSAASLLDKNNLLKYDKKSGNFQVTELGRIASHYYLTHETVSTFNNL 1004 HSP 3 Score: 70.8626 bits (172), Expect = 3.014e-17 Identity = 39/115 (33.91%), Postives = 63/115 (54.78%), Query Frame = 1
Query: 109 KISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIIL-TSSEEEFAHQF 444
+++++ L+ + G+ F H GL + +IV F GA+ V+ + TL + VNL AHLVV+ T + Y +L MIG A RP D++ A+IL +S++EF +F
Sbjct: 1603 RLTDKTLKETVTYGIAFLHEGLSDAEVKIVNQLFTSGAIQVVVVSRTLCLSVNLSAHLVVVMDTQFYEGKTHSYADYPVTDVLHMIGHASRPLLDDAGKAVILCQASKKEFFKKF 1717 HSP 4 Score: 37.7354 bits (86), Expect = 3.014e-17 Identity = 23/103 (22.33%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAE+ + + ++ +FLY R+ NP YY + G + + +L+ + ++ L ++ + ++++ TP G++ A Y++++ TI+
Sbjct: 1732 LHDHFNAEVVTKTIENKQDAVDYLTWTFLYRRMTLNPNYYNL-QGVTHRHLSDHMSELVENTLNDLEQSKCVS---VEDEMDVTPLNLGMIAAYYYINYTTIE 1830
BLAST of Helicase for meiosis 1 vs. Ensembl Nematostella
Match: EDO46714 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPC5]) HSP 1 Score: 85.1149 bits (209), Expect = 5.338e-27 Identity = 45/103 (43.69%), Postives = 61/103 (59.22%), Query Frame = 1
Query: 121 EKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSE 423
++LR G G HHAG+ + DR +VE F G + VL T+TL+ GVNLPAH V+IKGT +++ L LQ+ GRAGRPQFD I+T+ +
Sbjct: 774 KQLRDLFPDGFGTHHAGMLRTDRTMVEQYFAKGLIKVLVCTATLAWGVNLPAHAVIIKGTEVYDAKKGSFVDLGILDVLQIFGRAGRPQFDKFGEGTIITAHD 876 HSP 2 Score: 56.225 bits (134), Expect = 5.338e-27 Identity = 29/112 (25.89%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICE--AIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRK 757
+L +L + LNAEI L + +++ +W+ S+LYIR+ +NP YG+P + + +LI+S KL +I + + T G + +++ + TI++ +
Sbjct: 894 TLTASLTDSLNAEICLGTVTNVNEAVEWLSYSYLYIRMIKNPLVYGIPHKDKEDDPTLENYRRQLIVSTARKLDKARMIRFAEEQGYLSATDLGRTASHFYIKYDTIEEFNE 1005 HSP 3 Score: 96.6709 bits (239), Expect = 1.207e-23 Identity = 54/138 (39.13%), Postives = 78/138 (56.52%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNI--EQTYTQNKLLQMIGRAGRPQFDNSAVAIIL 411
TR + L FL D + L + ++ + + + L+ + G+G HHAGL + DR +VE F+ + VL +TSTL+ GVN PAHLVVIKGT F+ + +LQM+GRAGRPQ+D+ A+IL
Sbjct: 1573 TRLTALDLIAFLAAQDDPKQWLHMPE-EEMEQVLHAVRDSNLKLVLSFGIGLHHAGLHERDRSVVEELFVNQKIQVLIATSTLAWGVNFPAHLVVIKGTEYFDGKKCRYVDFPITDVLQMMGRAGRPQYDDQGKAVIL 1709 HSP 4 Score: 33.4982 bits (75), Expect = 1.207e-23 Identity = 19/105 (18.10%), Postives = 51/105 (48.57%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRK 757
L +HLNAE+ + ++ ++ + R+ NP YY + + +++ + + +L+ S +L +N + + + T + + Y+L+ T++ +++
Sbjct: 1736 LADHLNAEVVAGTITSKQDAMDYMTWTYFFRRLVMNPTYYDL-QDTDHDSVNKYLSRLVESAAAELELSGCLNTGEDSVSLSPTTAAHIASYYYLNHLTLRMLKE 1839
BLAST of Helicase for meiosis 1 vs. Ensembl Nematostella
Match: EDO44748 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT3]) HSP 1 Score: 75.8702 bits (185), Expect = 1.262e-13 Identity = 51/173 (29.48%), Postives = 91/173 (52.60%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEK------LRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNS--AVAIILTSSEEEFAHQFD*TFECGNRSSSFARYR 495
FC T+K+ +AK +V+ E+ +I + + Q + + L+ + G+ +HH+GL +R+++E ++ G L L TSTL+ GVNLPA V+++ NI + +++ QMIGRAGR D++ ++ II T+ + + A F F+ S + Y+
Sbjct: 273 FCATKKNCENVAKLMVEFLPREMTQVKIGERMELLQALHHQANGLCPVLKKSVPYGIAYHHSGLTMDERKLIEDAYSQGVLCALTCTSTLAAGVNLPAKRVILRA-PYIGRNIIR---KSQYKQMIGRAGRAGIDSTGESILIIKTTDKHKVAPLFSGEFDSCQSSLPYQEYK 441
BLAST of Helicase for meiosis 1 vs. Ensembl Nematostella
Match: EDO38196 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDN7]) HSP 1 Score: 67.0106 bits (162), Expect = 6.068e-11 Identity = 33/96 (34.38%), Postives = 55/96 (57.29%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIIL 411
+++ + RG+G HH+G+ + +E++E F G + +L +T T +MGVN+PA VV T + + + + +QM GRAGR D + IIL
Sbjct: 344 RMQELLQRGIGVHHSGILPILKEMIEILFQEGLVKLLYATETFAMGVNMPARTVVFDSTRKNDGSSFRDLLPGEYVQMAGRAGRRGKDTTGTVIIL 439
BLAST of Helicase for meiosis 1 vs. Ensembl Medaka
Match: hfm1 (helicase for meiosis 1 [Source:ZFIN;Acc:ZDB-GENE-061207-43]) HSP 1 Score: 140.584 bits (353), Expect = 7.924e-81 Identity = 90/278 (32.37%), Postives = 148/278 (53.24%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMH 1673
+ +S+ E SEI LR E+ +N+ NK + + FPLKG++ ++ MK++ L+Q LGC+ + D L Q+T KIFRIGLRI + FL + L++ +L KCF +KLW+N+ +VS Q+ +I ++ A + NAG +T +I + +E ILN+ PPFG + P+Y++ +EQ+ + V V Q S+ R Y + L+IG+ +N V + ++ L+ W +I V R S+ L ++LIS +
Sbjct: 518 VELVSKSKEFSEIQLRVNEKRPLNSFNKDKNRTTI-FPLKGKMKSSDMKVN-LIQAQLGCISIQDFGLTQDTAKIFRIGLRISKLSE---FLGQRSRAGFTALLNSIILAKCFQSKLWENSPYVSKQLEKIQTLSAA-MVNAGLTTFTKIEQINPRELELILNRHPPFGNQIRDSVMHLPKYDVCVEQLPRYNIASAEISVKVILKNQTQLLSKRTSPDRHY---VSLLIGNADNSVVCLHK-LDSILLKCGTWSKKIEVTRASKGDELNVNLISSQY 785 HSP 2 Score: 124.405 bits (311), Expect = 7.924e-81 Identity = 69/138 (50.00%), Postives = 87/138 (63.04%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILT 414
FC TRK Q A L RF L + QL + + + KLR + G+GFHHAGL DR++VE +F G L VL +T TL+MGVNLPAHLVVIK T + + Y++ +LQMIGRAGRPQFD SA A+I+T
Sbjct: 263 FCSTRKGAQQSAAILAKDVRFNLSIEH-KQLTKYANSVLDSKLRLVPL-GIGFHHAGLDLSDRKLVEEAFTQGDLSVL-TTRTLAMGVNLPAHLVVIKSTMQYVVGSCEEYSEADILQMIGRAGRPQFDTSATAVIMT 397 HSP 3 Score: 80.1073 bits (196), Expect = 7.924e-81 Identity = 48/121 (39.67%), Postives = 70/121 (57.85%), Query Frame = 2
Query: 428 SLHINLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
SLH +L+EHLNAEI L + D++ WI+S+FLYIR +NP +Y L + ++ LS+ LI+ D+ N I+ T G LMARY + F T++ K+ GSE+L+ LV
Sbjct: 417 SLHGHLVEHLNAEIVLQTISDVNMALDWIRSTFLYIRALKNPTHY-----------------LCLKNLNSLSSIDLISMDEDIN-IKPT-AGRLMARYCVAFDTMKQFSKVDGSESLSDLV 518
BLAST of Helicase for meiosis 1 vs. Ensembl Medaka
Match: snrnp200 (small nuclear ribonucleoprotein U5 subunit 200 [Source:NCBI gene;Acc:101154872]) HSP 1 Score: 88.5817 bits (218), Expect = 1.389e-31 Identity = 47/111 (42.34%), Postives = 64/111 (57.66%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D I++TS E
Sbjct: 688 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFSDRHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 790 HSP 2 Score: 53.1434 bits (126), Expect = 1.389e-31 Identity = 29/109 (26.61%), Postives = 54/109 (49.54%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LY+R+ RNP YG+ S + + M LI + + L SL+ +D+ T + T G + + +++ ++Q +L
Sbjct: 811 KLPDMLNAEIVLGNVQTVKDAVNWLGYTYLYVRMLRNPTLYGISQDDRSADPLLERRRMDLIHTAANVLDKNSLVKYDKRTGAFQVTDLGRIASHFYITHDSVQTYNQL 919 HSP 3 Score: 36.965 bits (84), Expect = 1.389e-31 Identity = 39/184 (21.20%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE IN+R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +T+ C ++ K ++W + + ++PE V ++ N F L + +++ L + P G+T+ K FPR +L +
Sbjct: 934 SLSSEFKNINVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTMNLCKMIDK----RMWQSMSPLRQFKKLPE-EVVKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHQFPRLDLAV 1103 HSP 4 Score: 78.9518 bits (193), Expect = 3.327e-19 Identity = 40/116 (34.48%), Postives = 68/116 (58.62%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIIL-TSSEEEFAHQF 444
+KI++ L+ + GVG+ H GL +R+IVE F GA+ V+ S+ +L G+++ AHLV++ T +N I Y +LQM+G+A RP D+ +I+ S+++F +F
Sbjct: 1517 EKINDPTLKETLANGVGYLHEGLSATERKIVEQLFNSGAVQVVVSSRSLCWGISISAHLVIVMDTQYYNGKIHAYVDYPIYDVLQMVGKANRPMLDDEGRCVIMCQGSKKDFFKKF 1632 HSP 5 Score: 37.7354 bits (86), Expect = 3.327e-19 Identity = 25/103 (24.27%), Postives = 52/103 (50.49%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1647 LHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGMSHRHLSDHLSELVENTLHDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1745
BLAST of Helicase for meiosis 1 vs. Ensembl Medaka
Match: snrnp200 (small nuclear ribonucleoprotein U5 subunit 200 [Source:NCBI gene;Acc:101154872]) HSP 1 Score: 88.5817 bits (218), Expect = 1.758e-31 Identity = 47/111 (42.34%), Postives = 64/111 (57.66%), Query Frame = 1
Query: 124 KLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNK----------LLQMIGRAGRPQFDNSAVAIILTSSEE 426
+L+ + G HHAG+ ++DR +VE F + VL ST+TL+ GVNLPAH V+IKGT Q Y+ K +LQM+GRAGRPQ+D I++TS E
Sbjct: 771 ELKDLLPYGFAIHHAGMTRVDRTLVEDLFSDRHIQVLVSTATLAWGVNLPAHTVIIKGT--------QVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGE 873 HSP 2 Score: 52.7582 bits (125), Expect = 1.758e-31 Identity = 29/108 (26.85%), Postives = 54/108 (50.00%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTG--SICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKL 760
L + LNAEI L + + W+ ++LY+R+ RNP YG+ S + + M LI + + L SL+ +D+ T + T G + + +++ ++Q +L
Sbjct: 895 LPDMLNAEIVLGNVQTVKDAVNWLGYTYLYVRMLRNPTLYGISQDDRSADPLLERRRMDLIHTAANVLDKNSLVKYDKRTGAFQVTDLGRIASHFYITHDSVQTYNQL 1002 HSP 3 Score: 36.965 bits (84), Expect = 1.758e-31 Identity = 39/184 (21.20%), Postives = 81/184 (44.02%), Query Frame = 3
Query: 852 SRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSH--QIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTI 1397
S SE IN+R E+ L + ++ P+K I S KI+ L+Q + L+++ +L + + + R++R + + + +T+ C ++ K ++W + + ++PE V ++ N F L + +++ L + P G+T+ K FPR +L +
Sbjct: 1017 SLSSEFKNINVREEEK-----LELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTMNLCKMIDK----RMWQSMSPLRQFKKLPE-EVVKKIEKKNFPFERLYDLNHNEIGE----LIRMPKMGKTIHKYVHQFPRLDLAV 1186 HSP 4 Score: 78.9518 bits (193), Expect = 3.982e-19 Identity = 40/116 (34.48%), Postives = 68/116 (58.62%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIIL-TSSEEEFAHQF 444
+KI++ L+ + GVG+ H GL +R+IVE F GA+ V+ S+ +L G+++ AHLV++ T +N I Y +LQM+G+A RP D+ +I+ S+++F +F
Sbjct: 1600 EKINDPTLKETLANGVGYLHEGLSATERKIVEQLFNSGAVQVVVSSRSLCWGISISAHLVIVMDTQYYNGKIHAYVDYPIYDVLQMVGKANRPMLDDEGRCVIMCQGSKKDFFKKF 1715 HSP 5 Score: 37.3502 bits (85), Expect = 3.982e-19 Identity = 25/103 (24.27%), Postives = 52/103 (50.49%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTP--CGLLMARYFLDFQTIQ 745
L +H NAEI + + ++ +FLY R+ +NP YY + G + + +L+ + + L I+ I ++++ P G++ A Y++++ TI+
Sbjct: 1730 LHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNL-QGMSHRHLSDHLSELVENTLHDLEQSKCIS---IEDEMDVAPLNLGMIAAYYYINYTTIE 1828
BLAST of Helicase for meiosis 1 vs. Ensembl Medaka
Match: ascc3 (activating signal cointegrator 1 complex subunit 3 [Source:NCBI gene;Acc:101173332]) HSP 1 Score: 85.1149 bits (209), Expect = 6.057e-26 Identity = 44/109 (40.37%), Postives = 65/109 (59.63%), Query Frame = 1
Query: 106 QKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKL--LQMIGRAGRPQFDNSAVAIILTSSEE 426
Q+ ++++ G G HHAG+ + DR ++ES F G L VL T+TL+ GVNLPAH V+IKGT +++ L +Q+ GRAGRPQFD I+T+ ++
Sbjct: 623 QRSRNKQMKEMFPEGFGIHHAGMLRSDRSLMESLFSKGYLKVLVCTATLAWGVNLPAHAVIIKGTQIYDAKRGAFVDLGILDVMQIFGRAGRPQFDKFGEGTIITTHDK 731 HSP 2 Score: 54.299 bits (129), Expect = 6.057e-26 Identity = 29/114 (25.44%), Postives = 62/114 (54.39%), Query Frame = 2
Query: 440 NLIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIM---KLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSE 772
+L ++LNAEIAL + +++ KW+ ++LY+R+ NP YG+ +I + P + + +L++ +L +I ++ T +T G + +++ + TI+ +L S+
Sbjct: 752 SLADNLNAEIALGTVTNVEEAVKWLSYTYLYVRMRANPLAYGINHKAI-QMDPTLELFRTELVVESGRRLDKAHMIRFEERTGYFASTDLGRTASHFYIRYNTIETFNELFSSQ 864 HSP 3 Score: 99.3673 bits (246), Expect = 2.260e-25 Identity = 57/150 (38.00%), Postives = 85/150 (56.67%), Query Frame = 1
Query: 10 TRKSTFQLAKFLV--DQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQ--TYTQNKLLQMIGRAGRPQFDNSAVAIILTSS-EEEFAHQF 444
TR + L +L D + L D+ ++ I + + L+ + G+G HHAGL + DR+ VE F+ + VL +TSTL+ GVN PAHLVV+KGT ++ + Y +LQM+GRAGRPQFD A+IL +++F +F
Sbjct: 1427 TRLTALDLIAYLATEDNPKQWLHQDE-REIEDIIATVRDSNLKLTLAFGIGLHHAGLHERDRKTVEELFVNCKIQVLVATSTLAWGVNFPAHLVVVKGTEYYDGKSRRYVDYPITDVLQMMGRAGRPQFDEQGKAVILVHDIKKDFYKKF 1575 HSP 4 Score: 38.1206 bits (87), Expect = 2.260e-25 Identity = 25/100 (25.00%), Postives = 46/100 (46.00%), Query Frame = 2
Query: 443 LIEHLNAEIALHPLLDIDSIYKWIQSSFLYIRIFRNPAYYGVPTGSICEAIPQIIMKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTI 742
L +HLNAEIA + +I ++ + R+ NP+YY + S E+I + + L+ + L I + +E G + + Y+L Q++
Sbjct: 1590 LSDHLNAEIAAGTISSKQDAMDYITWTYFFRRLVMNPSYYSLADTS-HESINKYLSNLVEQSLRDLEISHCIEIKEDERTVEPLTYGRIASYYYLKHQSV 1688
BLAST of Helicase for meiosis 1 vs. Ensembl Medaka
Match: sclt1 (sodium channel and clathrin linker 1 [Source:NCBI gene;Acc:101167852]) HSP 1 Score: 75.8702 bits (185), Expect = 2.764e-13 Identity = 158/708 (22.32%), Postives = 323/708 (45.62%), Query Frame = 1
Query: 3022 SEFDFLKDQVSRLNGVLSNYQEKY--------FEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLR--------NQIDNMPKNFETCFS----EQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQN------------EVTENNMKPQTVIF----------NEQMESMRQMYMNSIQELENE-----------------AVAN-RDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNXXXXXXXXXXXXXLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFT--KLLLASE------------ESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFXXXXXXXXXXWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLERKVNISEDMA------------PFNIE 4887
+E D+L+DQV RLN L YQ+ E++ + ++ L + PL+ EYD+ I +MT+ +++ + + ++ ++ + KENERL Q+ ++P T + + N+Q Q++L +E+ E+ + ++ E+DRLQ + +K Q V F N+++ES Q + ++ E +E A A ++ + L N E+ QR+ EL ++A + + AA+ L QL+ +L +++ Q EAV ++ W + +++ + E++ + A KV E ++AE+A + K +RE Q E E+ + +++A VRT++EV+ + + R+ +EL+ + + ++++ R + +E+E+E +++ R + E + + H R + AE+ +++ L LQ+ + + E N E S Q+++ L+ S A ++ + +L L E ++ +KK ++ + HEY + K+Q LE R+ E++ R +++++ + L+ QQ+ + + +EEA NL + +E K+ + E +RQK+R+ E+ + L I+E + + E + L+R++ +E A PF+++
Sbjct: 4 AELDYLRDQVRRLNLSLRQYQDGLHPPSTSSQVEEAWRAESSAPWLTDRSLMAPLIAEYDRHIADMTERLRKNQEQMTNLKVQLERVVKENERLHAELKECVEKQLHSLPVASGTSGGTMEEDAIIRNLQEQLELSEQERIKAMELWQTADQELDRLQRIHQQTVSDHEGQDAERRQLKDQLVQFQQHSYKFQVANQKLESTSQQLLKTVAEQSSEMEELHSQLRRAKAELRTATAKVEEMTKLLQNLQEQIQRREEEL-----LEA---QGRESAADRRLQQLQGTLSQLETRLTAASQEAEAVH----------REQAVWEKKVGELQARCTTLEEEKHQALSKVRENIQLAEEATLHKDQALLREKQKTEELEKTKEAFKLFIQEAAVRTRKEVENERKQCNIRIHRMSEELSVLQLECADKQSQIERSLRERKAVEEELERVYKEGRAEP--ELQKIDALHQRCLDAERLKDDALLTLQTTQKKLKKMEMEHN-------EELSRCQEEMRRLRSSLAAAHNDGVSVSEERLQLQHENLQLRREMEELRKATMLVQKKAKQEVSQMEHEYSV-------KEQGLEARVRELEESSRSSSADLTRLLAAQQKSAQRRKEEAKNLVQAFEAKITGLKMELNRQKQRSRELQAQLERDHDTIMEYEKQLGEHQEKAGRLQRRLTQAEQKAASAVQQVGPCRLPFHVQ 677
BLAST of Helicase for meiosis 1 vs. Planmine SMEST
Match: SMESG000065400.1 (SMESG000065400.1) HSP 1 Score: 1449.11 bits (3750), Expect = 0.000e+0 Identity = 707/723 (97.79%), Postives = 708/723 (97.93%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTNSKLDNKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRNMSLISSYRQSCSTTSFDHSKRKNTSPVIVSKSFKSACLQYPTQLSCGRDMSVDLDKSASFNEFSDDSFDEVMSKIDEESFNNSFIQIDNFPVSSTPIVKNLSSHNPMKNINANDFKSFNQMLNMNAESPILPVCAIERDINVPLVQTIKIGSHNNTQKSFEISPTTQNSSQKFVNSGFIRTKSYNSCWEMFSLPMTKSVRESDLLPTVNNGLLYTSQPIDKIPNTLTEWTDPIADRSLVDSINDLNTDMMHNPSTVEFPHLKIKSTGTSNSFSNPFLSSINSEDLRHINVGEKLLNVDSISHLDSYQNTELMDKSNAANISELSVHHSRQLGIVNGNPGTNIPSPSMVSFYNQGWDALFDFIKMRSLLNASKA 3008
ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQ ETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTNSKLDNKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRNMSLISSYRQSCSTTSFDHSKRKNTSPVIVSK+FKSACLQYPTQLSCGRDMSVDLDKSASFNEFSDDSFDEVMSKIDEESFNNSFIQIDNFPVSSTPIVKNLSSHNPMKNINANDFKSFNQMLNMNAESPILPVCAIERDINVPLVQTIKIGSHNNTQKSFEISPTTQNSSQKFVNSGFIRTKSYNSCWEMFSLPMTKSVRESDLLPTVNNGLLYTSQPIDKIPNTLTEWTDPIADRSLVDSINDLNTDMMHNPSTVEFPHLKIKSTGTSNSFSNPFLSSINSEDLRHINVGEKLLNVDSISHLDSYQNTELMDKSNAANISELSVHHSRQLGIVNGNPGTNIPSPSMVSFYNQGWDALFDFIKMRSLLNASKA
Sbjct: 61 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQ---------------ETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTNSKLDNKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRNMSLISSYRQSCSTTSFDHSKRKNTSPVIVSKNFKSACLQYPTQLSCGRDMSVDLDKSASFNEFSDDSFDEVMSKIDEESFNNSFIQIDNFPVSSTPIVKNLSSHNPMKNINANDFKSFNQMLNMNAESPILPVCAIERDINVPLVQTIKIGSHNNTQKSFEISPTTQNSSQKFVNSGFIRTKSYNSCWEMFSLPMTKSVRESDLLPTVNNGLLYTSQPIDKIPNTLTEWTDPIADRSLVDSINDLNTDMMHNPSTVEFPHLKIKSTGTSNSFSNPFLSSINSEDLRHINVGEKLLNVDSISHLDSYQNTELMDKSNAANISELSVHHSRQLGIVNGNPGTNIPSPSMVSFYNQGWDALFDFIKMRSLLNASKA 768 HSP 2 Score: 123.635 bits (309), Expect = 4.364e-28 Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 2
Query: 608 MKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
MKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSL+
Sbjct: 1 MKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLI 61
BLAST of Helicase for meiosis 1 vs. Planmine SMEST
Match: SMESG000065400.1 (SMESG000065400.1) HSP 1 Score: 1440.63 bits (3728), Expect = 0.000e+0 Identity = 707/732 (96.58%), Postives = 708/732 (96.72%), Query Frame = 3
Query: 840 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTNSKLD---------NKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRNMSLISSYRQSCSTTSFDHSKRKNTSPVIVSKSFKSACLQYPTQLSCGRDMSVDLDKSASFNEFSDDSFDEVMSKIDEESFNNSFIQIDNFPVSSTPIVKNLSSHNPMKNINANDFKSFNQMLNMNAESPILPVCAIERDINVPLVQTIKIGSHNNTQKSFEISPTTQNSSQKFVNSGFIRTKSYNSCWEMFSLPMTKSVRESDLLPTVNNGLLYTSQPIDKIPNTLTEWTDPIADRSLVDSINDLNTDMMHNPSTVEFPHLKIKSTGTSNSFSNPFLSSINSEDLRHINVGEKLLNVDSISHLDSYQNTELMDKSNAANISELSVHHSRQLGIVNGNPGTNIPSPSMVSFYNQGWDALFDFIKMRSLLNASKA 3008
ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQ ETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTNSKLD NKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRNMSLISSYRQSCSTTSFDHSKRKNTSPVIVSK+FKSACLQYPTQLSCGRDMSVDLDKSASFNEFSDDSFDEVMSKIDEESFNNSFIQIDNFPVSSTPIVKNLSSHNPMKNINANDFKSFNQMLNMNAESPILPVCAIERDINVPLVQTIKIGSHNNTQKSFEISPTTQNSSQKFVNSGFIRTKSYNSCWEMFSLPMTKSVRESDLLPTVNNGLLYTSQPIDKIPNTLTEWTDPIADRSLVDSINDLNTDMMHNPSTVEFPHLKIKSTGTSNSFSNPFLSSINSEDLRHINVGEKLLNVDSISHLDSYQNTELMDKSNAANISELSVHHSRQLGIVNGNPGTNIPSPSMVSFYNQGWDALFDFIKMRSLLNASKA
Sbjct: 61 ISFISRCSELSEINLRYTERGFMNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQ---------------ETDKIFRIGLRILRCVHDYLFLLSSDCDNYQTLVSCTLLKKCFVAKLWDNTWFVSHQIPEINSVMAVQLTNAGFSTLNRIANSQAQAIESILNKTPPFGQTVIKRASGFPRYELTIEQIGEKESNMCSLHVFVTRMRQKSDQSEVDCDIRFYNDSMLLIIGDLNNHVHYKSRIMNHQLVNSTVWRAEIIVNRISEDPTLCIDLISEMHVGFDIRTKFKPNYLNYIESQTKISKQNPEKFTNSKLDYDRNLLSKDNKLPSCPLDKNLTCDTPIATKVKVFPWKTCKISRNMSLISSYRQSCSTTSFDHSKRKNTSPVIVSKNFKSACLQYPTQLSCGRDMSVDLDKSASFNEFSDDSFDEVMSKIDEESFNNSFIQIDNFPVSSTPIVKNLSSHNPMKNINANDFKSFNQMLNMNAESPILPVCAIERDINVPLVQTIKIGSHNNTQKSFEISPTTQNSSQKFVNSGFIRTKSYNSCWEMFSLPMTKSVRESDLLPTVNNGLLYTSQPIDKIPNTLTEWTDPIADRSLVDSINDLNTDMMHNPSTVEFPHLKIKSTGTSNSFSNPFLSSINSEDLRHINVGEKLLNVDSISHLDSYQNTELMDKSNAANISELSVHHSRQLGIVNGNPGTNIPSPSMVSFYNQGWDALFDFIKMRSLLNASKA 777 HSP 2 Score: 123.25 bits (308), Expect = 4.679e-28 Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 2
Query: 608 MKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLV 790
MKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSL+
Sbjct: 1 MKLIISEVDKLSNGSLINHDQITNKIETTPCGLLMARYFLDFQTIQDIRKLSGSETLTSLI 61
BLAST of Helicase for meiosis 1 vs. Planmine SMEST
Match: SMESG000065404.1 (SMESG000065404.1) HSP 1 Score: 1279.23 bits (3309), Expect = 0.000e+0 Identity = 673/674 (99.85%), Postives = 673/674 (99.85%), Query Frame = 1
Query: 3010 MNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRNQIDNMPKNFETCFSEQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQNEVTENNMKPQTVIFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNXXXXXXXXXXXXXLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFTKLLLASEESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFXXXXXXXXXXWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLERKVNISEDMAPFNIEVDSSDLDNSGWXXXXXXXXXXRIVKESVARKFDFSPKFSCKPETSSFR 5031
MNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRNQIDNMPKNFETCFSEQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQNEVTENNMKPQTVIFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNRAIRAEKEREEIRLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFTKLLLASEESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFLSKQQQLSSKWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLE KVNISEDMAPFNIEVDSSDLDNSGWQLQQFKKQQQRIVKESVARKFDFSPKFSCKPETSSFR
Sbjct: 1 MNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRNQIDNMPKNFETCFSEQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQNEVTENNMKPQTVIFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNRAIRAEKEREEIRLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFTKLLLASEESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFLSKQQQLSSKWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLESKVNISEDMAPFNIEVDSSDLDNSGWQLQQFKKQQQRIVKESVARKFDFSPKFSCKPETSSFR 674
BLAST of Helicase for meiosis 1 vs. Planmine SMEST
Match: SMESG000065404.1 (SMESG000065404.1) HSP 1 Score: 1237.63 bits (3201), Expect = 0.000e+0 Identity = 655/656 (99.85%), Postives = 655/656 (99.85%), Query Frame = 1
Query: 3010 MNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRNQIDNMPKNFETCFSEQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQNEVTENNMKPQTVIFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNXXXXXXXXXXXXXLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFTKLLLASEESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFXXXXXXXXXXWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLERKVNISEDMAPFNIEVDSSDLDNSGWXXXXXXXXXXRIVKESVAR 4977
MNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRNQIDNMPKNFETCFSEQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQNEVTENNMKPQTVIFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNRAIRAEKEREEIRLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFTKLLLASEESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFLSKQQQLSSKWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLE KVNISEDMAPFNIEVDSSDLDNSGWQLQQFKKQQQRIVKESVAR
Sbjct: 1 MNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNNHTTPLLMEYDKVIFEMTKEIQEFKTYVEQEQMSVKAITKENERLRNQIDNMPKNFETCFSEQLKVNMQAQIDLMIKEKNSLQEMLRISELEVDRLQNEVTENNMKPQTVIFNEQMESMRQMYMNSIQELENEAVANRDIKRDLSNELEKQQRQNLELEKENKIQADKLKFEKEAAEENLNQLKSSLKRIDLLNNEKKQLLEAVDALRTEIKNHDSVKSEWCTTLSDIEVKYNEKEQQVYDASQKVSEAYKIAEDAAVEKKAIEIREMQALGECERLRGVISGILEDAGVRTKEEVDLVANKYEKEMERVMDELNFCEQNRQELIVELDQMKRSKSQLEQEMELLHRSDREKSFQENSQEEINHNRAIRAEKEREEIRLQLQSAENGFTRERAEWNRQREAFENENSFMQKKLSNLQMSCDKAQSNADRFTKLLLASEESATKNRKKYQESLRNCRHEYELLKQASQMKQQELETRLASAEDTQRHTTSEMRQFLSKQQQLSSKWREEAHNLSEQYEQKLKQITKEFDRQKKRNDEMISLLHESKQEILESHEVIDEQKRTIELLESKVNISEDMAPFNIEVDSSDLDNSGWQLQQFKKQQQRIVKESVAR 656
BLAST of Helicase for meiosis 1 vs. Planmine SMEST
Match: SMESG000065396.1 (SMESG000065396.1) HSP 1 Score: 295.819 bits (756), Expect = 8.384e-88 Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 1
Query: 1 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEEE 429
FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEEE
Sbjct: 322 FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGVGFHHAGLCQLDREIVESSFLCGALPVLCSTSTLSMGVNLPAHLVVIKGTSNFNSNIEQTYTQNKLLQMIGRAGRPQFDNSAVAIILTSSEEE 464 The following BLAST results are available for this feature:
BLAST of Helicase for meiosis 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Helicase for meiosis 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Helicase for meiosis 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030220 ID=SMED30030220|Name=Helicase for meiosis 1|organism=Schmidtea mediterranea sexual|type=transcript|length=5281bpback to top protein sequence of SMED30030220-orf-1 >SMED30030220-orf-1 ID=SMED30030220-orf-1|Name=SMED30030220-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp FCMTRKSTFQLAKFLVDQTRFELKFDQIHQLGVISQKISEEKLRACIIRGback to top protein sequence of SMED30030220-orf-2 >SMED30030220-orf-2 ID=SMED30030220-orf-2|Name=SMED30030220-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=709bp MNNLNKHRGPNKLRFPLKGRIMNASMKIDCLVQVILGCLEVDDSSLHQETback to top protein sequence of SMED30030220-orf-3 >SMED30030220-orf-3 ID=SMED30030220-orf-3|Name=SMED30030220-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=680bp MHQKHMNTQSEFDFLKDQVSRLNGVLSNYQEKYFEQSEKKGNNDIKLIFNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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