SMED30030203
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030203 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30030203 aligns in the following genomic locations:
Homology
BLAST of SMED30030203 vs. Planmine SMEST
Match: SMESG000026714.1 (SMESG000026714.1) HSP 1 Score: 886.33 bits (2289), Expect = 0.000e+0 Identity = 466/481 (96.88%), Postives = 473/481 (98.34%), Query Frame = 1
Query: 208 MKELRIRIVLFMXXXXXXXXNMSNYGIFAFHLDQPHSKATINLKFVNYYFCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIVSMIMITILLAIYEIQLLMLSSKVISYIIYFTFNIIQMIFLLVLILDTNGCTDTGENWFMNFMNTIAILISIAIQLHELMYNLSFKLTDIFCTIINYSSGNYTTDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFHWNWKYMLNLSPKNDQDSNVTFDMSKPFHLPLRIITATIFGIIYFGNDYYKHYRMTSDSSVLFYLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMSGATEIVAEVTLGTKSQEADYIKFHKYFSKSFXXXXXXXXXXXXXXXXXRVPSISGDNRLEFYIVSPLMLIIPHLHKLVSIFIKFTKFLPEGKFDMRIFMSGMICICVPLELLYHWYAWIFYCWVMISSYKE 1650
MKELRIRIVLFMLALLLALLNMSNYGIFAFHLDQPHSKATINLKFVNYYFCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPL+LYLICVPLIVSMIMITILLAIYEIQLLMLSSKVISYIIYFTF +IQMIFLLVLILDTN CTDTGENWFMNFMNT+AILISIAIQLHELMYNLSFKLTDIFCTIINYSSGNYTTDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFH NWKYMLNLSPKNDQDSNVTFDMSKPFHLPLRIITATIFGIIYFGNDYYKH+R+TSDSSVLFYLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVM GATEIVAEVTLGTKS EADYIKFHKYF+KSFSLIILA ESTLLSLLLERVPSISGDNRL FYIVSPLMLIIPHLHKLVSIFIKFTKFLPEGKFDMRIFMSGMICI VPLELLYHWYAWIFYCWV+ISSYKE
Sbjct: 1 MKELRIRIVLFMLALLLALLNMSNYGIFAFHLDQPHSKATINLKFVNYYFCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPLNLYLICVPLIVSMIMITILLAIYEIQLLMLSSKVISYIIYFTFEVIQMIFLLVLILDTNSCTDTGENWFMNFMNTVAILISIAIQLHELMYNLSFKLTDIFCTIINYSSGNYTTDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFHRNWKYMLNLSPKNDQDSNVTFDMSKPFHLPLRIITATIFGIIYFGNDYYKHHRLTSDSSVLFYLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMGGATEIVAEVTLGTKSPEADYIKFHKYFNKSFSLIILAFESTLLSLLLERVPSISGDNRLRFYIVSPLMLIIPHLHKLVSIFIKFTKFLPEGKFDMRIFMSGMICIYVPLELLYHWYAWIFYCWVIISSYKE 481
BLAST of SMED30030203 vs. Planmine SMEST
Match: SMESG000026722.1 (SMESG000026722.1) HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0 Identity = 365/489 (74.64%), Postives = 407/489 (83.23%), Query Frame = 1
Query: 184 FLHKENLFMKELRIRIVLFMXXXXXXXXNMSNYGIFAFHLDQPHSKATINLKFVNYYFCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIVSMIMITILLAIYEIQLLMLSSKVISYIIYFTFNIIQMIFLLVLILDTNGCTDTGENWFMNFMNTIAILISIAIQLHELMYNLSFKLTDIFCTIINYSSGNYTTDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFHWNWKYMLNLSPKNDQDSNVTFDMSKPFHLPLRIITATIFGIIYFGNDYYKHYRMTSDSSVLFYLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMSGATEIVAEVTLGTKSQEADYIKFHKYFSKSFXXXXXXXXXXXXXXXXXRVPSISGDNRLEFYIVSPLMLIIPHLHKLVSIFIKFTKFLPEGKFDMRIFMSGMICICVPLELLYHWYAWIFYCWVMISSYKE 1650
FLH ENLFMKEL IR++LFM LLL + + NYGIFAFHL S+ T NLK + YY +A++CFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIV MI++ IL A+Y Q + VI Y IYF II +I LLVLILDT+G TDTGENWFMNF N+ AIL++IA Q+ ELMYNLSFKLTDIF TIINYSSGNY+TDKYS F+YE+K M +K IA+MFHLTIPAYI+ TF NWK+MLNLSP+NDQDSNV D SKPFHLPLRIITA FGII+FG D+Y++Y +TSDS+VLFYL FVIDMF++ IVLIMISEIKFWPKCNTPKSLNFKN+FIYLGVYGVVM+ ATEIVAE+TLG+ EADYIKFHKYFSKSFSLIILALESTLLSLLLERVPSIS DNRL FYI SPLMLII HL+K V+ IKFTKFLP +F IFM GMICI PLELLYHWYAWIFY WVMISSYKE
Sbjct: 3 FLHVENLFMKELLIRMILFMSTLLLGIFTVLNYGIFAFHLYLSRSEVTYNLKAICYYLMDALYCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIVMMILVGILFALYGTQEDR-NFIVIDYSIYFALLIIHLISLLVLILDTSGWTDTGENWFMNFTNSFAILVTIATQIPELMYNLSFKLTDIFSTIINYSSGNYSTDKYSNFIYESKRHTMLYKGDIAMMFHLTIPAYIMTTFIRNWKHMLNLSPRNDQDSNVAIDRSKPFHLPLRIITAAFFGIIFFGKDFYQYYGLTSDSAVLFYLFEFVIDMFEVGIVLIMISEIKFWPKCNTPKSLNFKNVFIYLGVYGVVMNVATEIVAEMTLGSTIPEADYIKFHKYFSKSFSLIILALESTLLSLLLERVPSISSDNRLRFYIGSPLMLIISHLYKFVTTIIKFTKFLPYEQFS--IFMPGMICIYGPLELLYHWYAWIFYSWVMISSYKE 488
BLAST of SMED30030203 vs. Planmine SMEST
Match: SMESG000026709.1 (SMESG000026709.1) HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0 Identity = 363/489 (74.23%), Postives = 405/489 (82.82%), Query Frame = 1
Query: 184 FLHKENLFMKELRIRIVLFMXXXXXXXXNMSNYGIFAFHLDQPHSKATINLKFVNYYFCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIVSMIMITILLAIYEIQLLMLSSKVISYIIYFTFNIIQMIFLLVLILDTNGCTDTGENWFMNFMNTIAILISIAIQLHELMYNLSFKLTDIFCTIINYSSGNYTTDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFHWNWKYMLNLSPKNDQDSNVTFDMSKPFHLPLRIITATIFGIIYFGNDYYKHYRMTSDSSVLFYLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMSGATEIVAEVTLGTKSQEADYIKFHKYFSKSFXXXXXXXXXXXXXXXXXRVPSISGDNRLEFYIVSPLMLIIPHLHKLVSIFIKFTKFLPEGKFDMRIFMSGMICICVPLELLYHWYAWIFYCWVMISSYKE 1650
FLH ENLF KEL IR+VLFM LL + +S+YG FAFHL S+ T NLK + YY EA++CFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIV MI++ I A+YE Q + K I Y IYF II +I LLVLILDT G TDTGENWFMNFMN+ AILISIA Q+ +LMYNLSFKLT I CTIINYSSGNY+TDKYS F+YE+K+ IM +K I++MFHLTIPAYI+ TF NWK+MLNLSP+N+QDSNV D SKPFHLPLRIITA FGII+FG D+YK+Y +TSDS+VLFYL FVIDMFQIVIVLI+ISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVM+ ATEIVAE+TLGT +ADYIKFHKYFSKSFSLIILALESTLLSLLLERVPSIS +N L+ YI SP MLII H++K V+ IKFTKFLP +F IFM GMICI PLELLYHWYAWIFY WVMISSYKE
Sbjct: 3 FLHVENLFTKELLIRMVLFMSMFLLGMFTVSSYGTFAFHLYLSQSEVTYNLKAICYYLIEALYCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLIVMMILVGIRFALYETQKDT-NFKFIDYSIYFALLIIHLISLLVLILDTRGYTDTGENWFMNFMNSFAILISIATQISQLMYNLSFKLTGILCTIINYSSGNYSTDKYSNFIYESKQHIMLYKGEISMMFHLTIPAYIMTTFIRNWKHMLNLSPRNNQDSNVAIDRSKPFHLPLRIITAAFFGIIFFGKDFYKYYGLTSDSAVLFYLFEFVIDMFQIVIVLIIISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMNVATEIVAEMTLGTTIPKADYIKFHKYFSKSFSLIILALESTLLSLLLERVPSISSENGLKLYIGSPSMLIITHVYKFVTTIIKFTKFLPYEQFS--IFMPGMICIYGPLELLYHWYAWIFYSWVMISSYKE 488
BLAST of SMED30030203 vs. Planmine SMEST
Match: SMESG000025344.1 (SMESG000025344.1) HSP 1 Score: 120.553 bits (301), Expect = 7.749e-29 Identity = 98/346 (28.32%), Postives = 172/346 (49.71%), Query Frame = 1
Query: 271 MSNYGIFAFHLDQPHSKATINLKFVNYYFCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVPLI-VSMIMITILLAIYEIQLLMLSSKVISYIIYFTFNIIQMIFLLVLILDTNGCTDTGENWFMNFMNTIAILISIAIQLHELMYNLSFKLTDIFCTIINYSS--GNYTTDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFHWNWKYMLNLSPKNDQDSNVT-FDMSKPFHLPLRIITA--TIFGIIYFGNDYYKHYRMTSDSSVLF--YLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMSGATEIVAEV 1284
++NYG + + + + L +NY V +IILI YN ++N +RNLRIK + E PL YLIC+P++ +S++ + +++ Y + S + I+YF I + ++L++D NG +DTGE WFMN+ N++ I+I++ + ++ N+ LTD F +N ++ ++ ++IF ++ K+ I L ++V N ++ PK D + T M+K + IT T+ + F + Y M + V+ +I ++ F ++ IMI+EIK + N+ + LN KNI IYLG +G T IV +
Sbjct: 32 VTNYGFYVYQISPRGDRKDEYLDKINYS-ISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPMLFISLMGLFVIIFHYTFE----KSGTSTNILYFALVIFEYFLFIILVIDINGFSDTGEEWFMNYFNSMTIIITMTSVMIQICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNK-----VSSITGGFTVGVFLTFIVIVHLLYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNI 367
BLAST of SMED30030203 vs. Planmine SMEST
Match: SMESG000025336.1 (SMESG000025336.1) HSP 1 Score: 119.013 bits (297), Expect = 2.573e-28 Identity = 108/361 (29.92%), Postives = 182/361 (50.42%), Query Frame = 1
Query: 271 MSNYGIFAFHLDQPHSKATINLKFVNYY--FCEAVFCFNIILIFYNQKVNFNYRNLRIKSRTESPLSLYLICVP-LIVSMIMITILLAIYEIQLLMLSSKVISYIIYFTFNIIQMIFLLVLILDTNGCTDTGENWFMNFMNTIAILISIAIQLHELMYNLSFKLTDIFCTIIN-------YSSGNYT-TDKYSIFLYENKEIIMSFKEIIAIMFHLTIPAYIVNTFHWNWKYMLNLSPKND-----------QDSNVTFDMSKPFHLPLR-IITATIFGIIYFGNDYYKHYRMTSDSSVLFYLIVFVIDMFQIVIVLIMISEIKFWPKCNTPKSLNFKNIFIYLGVYGVVMSGATEIVAEV 1284
++NYG + + + ++ ++ +NY F + V +IILI YN K+N +RNLRIK + E PL YLIC+P L++S+I + +++ Y + S K S+I Y + I + +VL++D NG +DTGE WFMN+ N++ I+I++ + ++ N+ LT+ F +N YS+ ++T DK S NK I + + +I + L ++V N ++ K+D + S++T ++ ++ L I+ F I GN Y R+ +LI+F I + IMI+EIK + N+ + LN KNI IYLG +G IV ++
Sbjct: 32 VTNYGFYVYRITPNENRNDGYIEKINYSISFVKVV---SIILICYNTKINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFER---SVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSVIMQICLNIIMILTEEFSNSLNQVLHFNLYSNHSFTFFDKTS----YNKIIFLKKRTVITL--SLAFIPFLVIPLKRNLNHLFQFKTKDDLPEDQPILSMNKVSSITGSLTVGVYITLIFIVDLCNFVIHERGNYIYLTSRIIKA-----FLILFSIGLLA-----IMITEIKVSARLNSSQYLNVKNILIYLGAFGQFSISIAYIVVDI 370 The following BLAST results are available for this feature:
BLAST of SMED30030203 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030203 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030203 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030203 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030203 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030203 ID=SMED30030203|Name=SMED30030203|organism=Schmidtea mediterranea sexual|type=transcript|length=1797bpback to top protein sequence of SMED30030203-orf-1 >SMED30030203-orf-1 ID=SMED30030203-orf-1|Name=SMED30030203-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=496bp MFLHKENLFMKELRIRIVLFMLALLLALLNMSNYGIFAFHLDQPHSKATIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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