SMED30030183
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030183 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SMED30030183 vs. Planmine SMEST
Match: SMESG000080881.1 (SMESG000080881.1) HSP 1 Score: 345.51 bits (885), Expect = 2.087e-168 Identity = 174/263 (66.16%), Postives = 214/263 (81.37%), Query Frame = 2 Query: 35 KDRVNLQGQIQCHSYKGYGHMARSCPKNSNKFQRPILKKIRFQNNELQSDNVAFVQSDDFNERIENEMKQIRSN*KRLDRLMCKFESDEEFDNYMVYDLQLEWSRTESKVKSAKDDLESEKVTMIIMVIKNLMASRALKTSKIMRSLNELSQMSLSVRKLKSVIELCAD--NENSKDRNEIENMMKIFGKAFLENAGFNFVFIDIVDRNNNSMIFVHCEAGDYVRDLKEKLKENNRKRIIELKFKNRCLNYDEFLIDCGFCTG 817 +DRVNLQG+IQCHS +GYGHMARSCP+N+ +FQ+PILKKIRFQ+++ Q+DNVAFVQ D+FN RIE+E+KQ+ +RLDRL+CKFESDEE DN+M+Y+ Q E S TE K KDDL+SEK MII I L+ SRA +TSKIM SL ELSQMSLSV++LK+VIELCAD N KDR+EI NMMKIFGKAF EN +F+FID+VD++ NS+I + CE GDYV+DLKE+LKENN ++II++KFKNR L DEFLIDCGF G Sbjct: 1790 RDRVNLQGRIQCHSCRGYGHMARSCPRNNERFQKPILKKIRFQSDKSQADNVAFVQPDNFNNRIEDEVKQMS---RRLDRLICKFESDEETDNHMMYESQSEASETELKKMMIKDDLQSEKAIMIISRINKLIESRAAETSKIMTSLLELSQMSLSVQRLKNVIELCADYYEVNLKDRDEISNMMKIFGKAFRENKWLDFIFIDVVDKSRNSVICIRCEIGDYVKDLKERLKENNGRQIIKVKFKNRYLEDDEFLIDCGFIPG 2049 HSP 2 Score: 231.876 bits (590), Expect = 2.087e-168 Identity = 110/147 (74.83%), Postives = 130/147 (88.44%), Query Frame = 1 Query: 1000 YIYHRSKYRERLKCLLRRFGKELRETRVELKSFLVALDYKVDGKTEFVDEHWCTKDRKLRKSAAEKLVNNGIII-MFKGEELDHDEYLYDCGFKPQELDEN*YLDADGRAGYFVNCVVAIKEQNVDSVNNLENSNTDIEEDNVNGIE 1437 Y+Y +S+Y E+LKCL+RRFG+ELRETR+ELK F++A+DY+ GKTE+++EHWCTK +KLRK AA ++ +GII+ MFK EELD DEYLYDCGFKPQELDEN YLDADGRAGY VNCVVAIKEQ VDSV+N ENSNTDIEEDNVNGIE Sbjct: 2109 YLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNVNGIE 2255 HSP 3 Score: 57.3806 bits (137), Expect = 2.087e-168 Identity = 31/57 (54.39%), Postives = 39/57 (68.42%), Query Frame = 3 Query: 807 FVPGNKNKLQIACMISENEVEKSDSQEIIKEFRDALRKWNEI*IFEMLKVIEKTELD 977 F+PGNKNKLQ+ACM E +KSDSQEII E W+ + I E LK+I+K EL+ Sbjct: 2046 FIPGNKNKLQVACMSKEEVAKKSDSQEIIGE-------WDGVKITEKLKLIDKIELE 2095
BLAST of SMED30030183 vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1) HSP 1 Score: 275.018 bits (702), Expect = 7.593e-151 Identity = 156/275 (56.73%), Postives = 196/275 (71.27%), Query Frame = 2 Query: 11 SINVTVTIKDRVNLQGQIQCHSYKGYGHMARSCPKNSNKFQRPIL----KKIRFQNNELQSDNVAFVQSDDFNERIENEMKQIRSN*KRLDRLMCKFESDEEFDNYMVYDLQLEWSRTESKVKSAKDDLESEKVTMIIMVIKNLMASRALKTSKIMRSLNELSQMSLSVRKLKSVIELCAD--NENSKDRNEIENMMKIFGKAFLENAGFNFVFIDIVDRNNNSMIFVHCEAGDYVRDLKEKLKENNRKRIIELKFKNRCLNYDEFLIDCGFCTG 817 S N T R+N IQC++ GH++R+C S +KIRFQ+++ Q+DNVAFVQ D+FN RIE+E+KQ+ + LDRL+CKFESDEE DN+M+Y+ Q E S TE K KDDL+SEK MI+ I L+ SRA +TSKIM SL ELSQ+SLSV++LKSVIELCAD N KDR+EI NMMKIFGKAF EN +F+FID+VD + NS+I + CE GDYV+DLKE+LKENN ++II++KFKNR L DEFLIDCGF G Sbjct: 335 SFNKMSTNSQRIN--DNIQCYNCGKRGHVSRNCFARSRSPSTSPSPQRDRKIRFQSDKSQADNVAFVQPDNFNNRIEDEVKQMS---RHLDRLICKFESDEETDNHMMYESQSEASDTEFKKIMIKDDLQSEKAIMIMSRINKLIESRATETSKIMTSLLELSQISLSVQRLKSVIELCADYYEVNLKDRDEISNMMKIFGKAFRENKWLDFIFIDVVDESRNSVICILCEIGDYVKDLKERLKENNGRQIIKVKFKNRYLEDDEFLIDCGFIPG 604 HSP 2 Score: 228.024 bits (580), Expect = 7.593e-151 Identity = 107/147 (72.79%), Postives = 128/147 (87.07%), Query Frame = 1 Query: 1000 YIYHRSKYRERLKCLLRRFGKELRETRVELKSFLVALDYKVDGKTEFVDEHWCTKDRKLRKSAAEKLVNNGIII-MFKGEELDHDEYLYDCGFKPQELDEN*YLDADGRAGYFVNCVVAIKEQNVDSVNNLENSNTDIEEDNVNGIE 1437 Y+Y +S+Y E+LKCL+RRFG+ELRETR+ELK F++A+DY+ GKTE+++EHWCTK +KLRK AA ++ +GII+ MFK EELD DEYLYDCGFKPQELDEN YLD DGRAGY VNCVVAIKEQ VD V+N ENSNTDIEEDN+NGIE Sbjct: 671 YLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDGDGRAGYLVNCVVAIKEQKVDPVDNSENSNTDIEEDNINGIE 817 HSP 3 Score: 73.559 bits (179), Expect = 7.593e-151 Identity = 35/57 (61.40%), Postives = 45/57 (78.95%), Query Frame = 3 Query: 807 FVPGNKNKLQIACMISENEVEKSDSQEIIKEFRDALRKWNEI*IFEMLKVIEKTELD 977 F+PGNKNKLQ+ACMI E EKSDSQ+II + R+AL +W+ + I E LK+I+K ELD Sbjct: 601 FIPGNKNKLQVACMIREEVAEKSDSQKIIGDLREALNEWDGVKITEKLKLIDKIELD 657
BLAST of SMED30030183 vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1) HSP 1 Score: 275.018 bits (702), Expect = 8.387e-151 Identity = 156/275 (56.73%), Postives = 196/275 (71.27%), Query Frame = 2 Query: 11 SINVTVTIKDRVNLQGQIQCHSYKGYGHMARSCPKNSNKFQRPIL----KKIRFQNNELQSDNVAFVQSDDFNERIENEMKQIRSN*KRLDRLMCKFESDEEFDNYMVYDLQLEWSRTESKVKSAKDDLESEKVTMIIMVIKNLMASRALKTSKIMRSLNELSQMSLSVRKLKSVIELCAD--NENSKDRNEIENMMKIFGKAFLENAGFNFVFIDIVDRNNNSMIFVHCEAGDYVRDLKEKLKENNRKRIIELKFKNRCLNYDEFLIDCGFCTG 817 S N T R+N IQC++ GH++R+C S +KIRFQ+++ Q+DNVAFVQ D+FN RIE+E+KQ+ + LDRL+CKFESDEE DN+M+Y+ Q E S TE K KDDL+SEK MI+ I L+ SRA +TSKIM SL ELSQ+SLSV++LKSVIELCAD N KDR+EI NMMKIFGKAF EN +F+FID+VD + NS+I + CE GDYV+DLKE+LKENN ++II++KFKNR L DEFLIDCGF G Sbjct: 335 SFNKMSTNSQRIN--DNIQCYNCGKRGHVSRNCFARSRSPSTSPSPQRDRKIRFQSDKSQADNVAFVQPDNFNNRIEDEVKQMS---RHLDRLICKFESDEETDNHMMYESQSEASDTEFKKIMIKDDLQSEKAIMIMSRINKLIESRATETSKIMTSLLELSQISLSVQRLKSVIELCADYYEVNLKDRDEISNMMKIFGKAFRENKWLDFIFIDVVDESRNSVICILCEIGDYVKDLKERLKENNGRQIIKVKFKNRYLEDDEFLIDCGFIPG 604 HSP 2 Score: 227.639 bits (579), Expect = 8.387e-151 Identity = 107/147 (72.79%), Postives = 128/147 (87.07%), Query Frame = 1 Query: 1000 YIYHRSKYRERLKCLLRRFGKELRETRVELKSFLVALDYKVDGKTEFVDEHWCTKDRKLRKSAAEKLVNNGIII-MFKGEELDHDEYLYDCGFKPQELDEN*YLDADGRAGYFVNCVVAIKEQNVDSVNNLENSNTDIEEDNVNGIE 1437 Y+Y +S+Y E+LKCL+RRFG+ELRETR+ELK F++A+DY+ GKTE+++EHWCTK +KLRK AA ++ +GII+ MFK EELD DEYLYDCGFKPQELDEN YLD DGRAGY VNCVVAIKEQ VD V+N ENSNTDIEEDN+NGIE Sbjct: 671 YLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDGDGRAGYLVNCVVAIKEQKVDPVDNSENSNTDIEEDNINGIE 817 HSP 3 Score: 73.559 bits (179), Expect = 8.387e-151 Identity = 35/57 (61.40%), Postives = 45/57 (78.95%), Query Frame = 3 Query: 807 FVPGNKNKLQIACMISENEVEKSDSQEIIKEFRDALRKWNEI*IFEMLKVIEKTELD 977 F+PGNKNKLQ+ACMI E EKSDSQ+II + R+AL +W+ + I E LK+I+K ELD Sbjct: 601 FIPGNKNKLQVACMIREEVAEKSDSQKIIGDLREALNEWDGVKITEKLKLIDKIELD 657
BLAST of SMED30030183 vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1) HSP 1 Score: 274.633 bits (701), Expect = 9.904e-151 Identity = 156/275 (56.73%), Postives = 196/275 (71.27%), Query Frame = 2 Query: 11 SINVTVTIKDRVNLQGQIQCHSYKGYGHMARSCPKNSNKFQRPIL----KKIRFQNNELQSDNVAFVQSDDFNERIENEMKQIRSN*KRLDRLMCKFESDEEFDNYMVYDLQLEWSRTESKVKSAKDDLESEKVTMIIMVIKNLMASRALKTSKIMRSLNELSQMSLSVRKLKSVIELCAD--NENSKDRNEIENMMKIFGKAFLENAGFNFVFIDIVDRNNNSMIFVHCEAGDYVRDLKEKLKENNRKRIIELKFKNRCLNYDEFLIDCGFCTG 817 S N T R+N IQC++ GH++R+C S +KIRFQ+++ Q+DNVAFVQ D+FN RIE+E+KQ+ + LDRL+CKFESDEE DN+M+Y+ Q E S TE K KDDL+SEK MI+ I L+ SRA +TSKIM SL ELSQ+SLSV++LKSVIELCAD N KDR+EI NMMKIFGKAF EN +F+FID+VD + NS+I + CE GDYV+DLKE+LKENN ++II++KFKNR L DEFLIDCGF G Sbjct: 335 SFNKMSTNSQRIN--DNIQCYNCGKRGHVSRNCFARSRSPSTSPSPQRDRKIRFQSDKSQADNVAFVQPDNFNNRIEDEVKQMS---RHLDRLICKFESDEETDNHMMYESQSEASDTEFKKIMIKDDLQSEKAIMIMSRINKLIESRATETSKIMTSLLELSQISLSVQRLKSVIELCADYYEVNLKDRDEISNMMKIFGKAFRENKWLDFIFIDVVDESRNSVICILCEIGDYVKDLKERLKENNGRQIIKVKFKNRYLEDDEFLIDCGFIPG 604 HSP 2 Score: 227.639 bits (579), Expect = 9.904e-151 Identity = 107/147 (72.79%), Postives = 128/147 (87.07%), Query Frame = 1 Query: 1000 YIYHRSKYRERLKCLLRRFGKELRETRVELKSFLVALDYKVDGKTEFVDEHWCTKDRKLRKSAAEKLVNNGIII-MFKGEELDHDEYLYDCGFKPQELDEN*YLDADGRAGYFVNCVVAIKEQNVDSVNNLENSNTDIEEDNVNGIE 1437 Y+Y +S+Y E+LKCL+RRFG+ELRETR+ELK F++A+DY+ GKTE+++EHWCTK +KLRK AA ++ +GII+ MFK EELD DEYLYDCGFKPQELDEN YLD DGRAGY VNCVVAIKEQ VD V+N ENSNTDIEEDN+NGIE Sbjct: 671 YLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDGDGRAGYLVNCVVAIKEQKVDPVDNSENSNTDIEEDNINGIE 817 HSP 3 Score: 73.559 bits (179), Expect = 9.904e-151 Identity = 35/57 (61.40%), Postives = 45/57 (78.95%), Query Frame = 3 Query: 807 FVPGNKNKLQIACMISENEVEKSDSQEIIKEFRDALRKWNEI*IFEMLKVIEKTELD 977 F+PGNKNKLQ+ACMI E EKSDSQ+II + R+AL +W+ + I E LK+I+K ELD Sbjct: 601 FIPGNKNKLQVACMIREEVAEKSDSQKIIGDLREALNEWDGVKITEKLKLIDKIELD 657
BLAST of SMED30030183 vs. Planmine SMEST
Match: SMESG000056661.1 (SMESG000056661.1) HSP 1 Score: 312.383 bits (799), Expect = 1.478e-141 Identity = 162/260 (62.31%), Postives = 199/260 (76.54%), Query Frame = 2 Query: 38 DRVNLQGQIQCHSYKGYGHMARSCPKNSNKFQRPILKKIRFQNNELQSDNVAFVQSDDFNERIENEMKQIRSN*KRLDRLMCKFESDEEFDNYMVYDLQLEWSRTESKVKSAKDDLESEKVTMIIMVIKNLMASRALKTSKIMRSLNELSQMSLSVRKLKSVIELCADNENSKDRNEIENMMKIFGKAFLENAGFNFVFIDIVDRNNNSMIFVHCEAGDYVRDLKEKLKENNRKRIIELKFKNRCLNYDEFLIDCGFCTG 817 DRVNLQG IQCHS +GYGHMARSCP+N+ +FQ+PILKKIRFQ+++ Q DNVAFVQ D+ N RIE+E+KQ+ +RLDRL+CKFESDEE DN+M+Y+LQ E S TE K KDD +SEK MI+ I L+ SRA +TSKIM SL ELSQMSLSV++LK +R+EI NMMKIFGKAF EN +F+FID+VD + NS+I + CE GDYV+DLKE+LKENN ++II++KFKNR L DEFLIDCGF G Sbjct: 304 DRVNLQGGIQCHSCRGYGHMARSCPRNNERFQKPILKKIRFQSDKSQVDNVAFVQPDNSNNRIEDEVKQMS---RRLDRLICKFESDEEKDNHMMYELQSEASDTELKNMMIKDDQQSEKEIMIMSRINKLIESRAAETSKIMTSLLELSQMSLSVQRLK-------------NRDEITNMMKIFGKAFRENKWLDFIFIDVVDESRNSVICIRCEIGDYVKDLKERLKENNGRQIIKVKFKNRYLEDDEFLIDCGFIPG 547 HSP 2 Score: 160.999 bits (406), Expect = 1.478e-141 Identity = 81/146 (55.48%), Postives = 98/146 (67.12%), Query Frame = 1 Query: 1000 YIYHRSKYRERLKCLLRRFGKELRETRVELKSFLVALDYKVDGKTEFVDEHWCTKDRKLRKSAAEKLVNNGIIIMFKGEELDHDEYLYDCGFKPQELDEN*YLDADGRAGYFVNCVVAIKEQNVDSVNNLENSNTDIEEDNVNGIE 1437 Y+Y +S+Y E+LKCL+RRFG+ELRET +ELK F++A+DY+ GK E+++EHWCTK +K F PQELDEN YLDADGR GY VNCVVA+KEQ +D V NLENSNTDIEEDNVNGIE Sbjct: 614 YLYDKSRYGEKLKCLMRRFGRELRETGMELKPFIIAIDYEDWGKAEYIEEHWCTKVQK---------------------------------FNPQELDENGYLDADGRVGYLVNCVVALKEQKIDLVYNLENSNTDIEEDNVNGIE 726 HSP 3 Score: 72.4034 bits (176), Expect = 1.478e-141 Identity = 33/57 (57.89%), Postives = 46/57 (80.70%), Query Frame = 3 Query: 807 FVPGNKNKLQIACMISENEVEKSDSQEIIKEFRDALRKWNEI*IFEMLKVIEKTELD 977 F+PGNKNKLQ+ACMI E +EKSDSQEI ++ RDAL +W+ + I E L++++K EL+ Sbjct: 544 FIPGNKNKLQVACMIREEVMEKSDSQEITRDLRDALSEWDGVKITEKLELLDKIELE 600 The following BLAST results are available for this feature:
BLAST of SMED30030183 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030183 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030183 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030183 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030183 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030183 ID=SMED30030183|Name=SMED30030183|organism=Schmidtea mediterranea sexual|type=transcript|length=1438bpback to top protein sequence of SMED30030183-orf-1 >SMED30030183-orf-1 ID=SMED30030183-orf-1|Name=SMED30030183-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=182bp MCKFESDEEFDNYMVYDLQLEWSRTESKVKSAKDDLESEKVTMIIMVIKNback to top Annotated Terms
The following terms have been associated with this transcript:
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