bcl2-like protein

Overview
Namebcl2-like protein (ACW83059.1)
Smed IDSMED30030127
Length (bp)1636
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of bcl2-like protein (SMED30030127) t-SNE clustered cells

Violin plots show distribution of expression levels for bcl2-like protein (SMED30030127) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of bcl2-like protein (SMED30030127) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for bcl2-like protein (SMED30030127) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030127

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30030127h1SMcG0016447 Contig210GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
dorsal ventral margin of the whole animalSMED30030127h1SMcG0016447 Contig210GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30030127h1SMcG0016447 Contig210GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
cephalic gangliaSMED30030127h1SMcG0016447 dd_Smed_v4_1018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30030127h1SMcG0016447 dd_Smed_v4_1018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30030127h1SMcG0016447 FJ807655smed_ncbi_20240424PMID:19766622
Pellettieri et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30030127h1SMcG0016447 FJ807655smed_ncbi_20240424PMID:19766622
Pellettieri et al., 2010		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of bcl2-like protein vs. Ensembl Human
Match: BCL2L1 (BCL2 like 1 [Source:HGNC Symbol;Acc:HGNC:992])

HSP 1 Score: 49.2914 bits (116), Expect = 5.911e-6
Identity = 37/145 (25.52%), Postives = 64/145 (44.14%), Query Frame = 1 
Query:  271 IVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMY-----IIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGK---VALGITGLFALGK 681
            + Q L     E + R +   S+ +S L+  T     QSF  VV  LF D + W R++  F +     +  V K  +    +   W    +   + PW++ENGGW+  V++  N N   E  +  ++F +     + + G+  LG 
Sbjct:   86 VKQALREAGDEFELRYRRAFSDLTSQLHI-TPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGN-NAAAESRKGQERFNRWFLTGMTVAGVVLLGS 228
[Close]
BLAST of bcl2-like protein vs. Ensembl Human
Match: BCL2L1 (BCL2 like 1 [Source:HGNC Symbol;Acc:HGNC:992])

HSP 1 Score: 49.2914 bits (116), Expect = 5.911e-6
Identity = 37/145 (25.52%), Postives = 64/145 (44.14%), Query Frame = 1 
Query:  271 IVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMY-----IIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGK---VALGITGLFALGK 681
            + Q L     E + R +   S+ +S L+  T     QSF  VV  LF D + W R++  F +     +  V K  +    +   W    +   + PW++ENGGW+  V++  N N   E  +  ++F +     + + G+  LG 
Sbjct:   86 VKQALREAGDEFELRYRRAFSDLTSQLHI-TPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGN-NAAAESRKGQERFNRWFLTGMTVAGVVLLGS 228
[Close]
BLAST of bcl2-like protein vs. Ensembl Zebrafish
Match: bcl2b (BCL2 apoptosis regulator b [Source:ZFIN;Acc:ZDB-GENE-110411-185])

HSP 1 Score: 53.1434 bits (126), Expect = 1.654e-7
Identity = 40/144 (27.78%), Postives = 65/144 (45.14%), Query Frame = 1 
Query:  268 DIVQCLHSLVVEAQDRGKDTI-SNFSSILNK--PTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVL-----KISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQI-SKNRNHL--EEVTETMKQFGKVALGITGL 666
            D    LH+++ EA D  +    S+F+ +  +   T     Q F  V++ LF D + W R+I  F +   V      K    +      W  + +   ++ W+RENGGWE  V++ S  R+ +     +  +  FG  ALG  GL
Sbjct:   57 DPYSALHNVLREAGDELESLYQSDFAEMSKQLHVTSITAHQRFNAVIDELFRDGVNWGRIIAFFEFGGTVCAECVNKEMTGQVDNIAVWMADYLNGPLHGWIRENGGWEAFVELYSSQRDSVFHSSWSSIVTVFGLAALGAVGL 200
[Close]
BLAST of bcl2-like protein vs. Ensembl Mouse
Match: Bcl2l1 (BCL2-like 1 [Source:MGI Symbol;Acc:MGI:88139])

HSP 1 Score: 48.9062 bits (115), Expect = 5.970e-6
Identity = 37/145 (25.52%), Postives = 63/145 (43.45%), Query Frame = 1 
Query:  271 IVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMY-----IIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGK---VALGITGLFALGK 681
            + Q L     E + R +   S+ +S L+  T     QSF  VV  LF D + W R++  F +     +  V K  +    +   W    +   + PW++ENGGW+  V +  N N   E  +  ++F +     + + G+  LG 
Sbjct:   86 VKQALREAGDEFELRYRRAFSDLTSQLHI-TPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGN-NAAAESRKGQERFNRWFLTGMTVAGVVLLGS 228
[Close]
BLAST of bcl2-like protein vs. Ensembl Mouse
Match: Bcl2l1 (BCL2-like 1 [Source:MGI Symbol;Acc:MGI:88139])

HSP 1 Score: 48.9062 bits (115), Expect = 5.970e-6
Identity = 37/145 (25.52%), Postives = 63/145 (43.45%), Query Frame = 1 
Query:  271 IVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMY-----IIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGK---VALGITGLFALGK 681
            + Q L     E + R +   S+ +S L+  T     QSF  VV  LF D + W R++  F +     +  V K  +    +   W    +   + PW++ENGGW+  V +  N N   E  +  ++F +     + + G+  LG 
Sbjct:   86 VKQALREAGDEFELRYRRAFSDLTSQLHI-TPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGN-NAAAESRKGQERFNRWFLTGMTVAGVVLLGS 228
[Close]
BLAST of bcl2-like protein vs. UniProt/SwissProt
Match: sp|Q91828|AR11_XENLA (Apoptosis regulator R11 OS=Xenopus laevis OX=8355 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.962e-10
Identity = 41/146 (28.08%), Postives = 75/146 (51.37%), Query Frame = 1 
Query:  259 LNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNE-----YKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVA--LGITGLFAL 675
            + ++++Q L     E + R +   S+ +S L+  T D  +QSF  V+  LF D   W R++  F +   +   S N+       +  +W V  +   + PW++ENGGWE  V +   +N   +  E+ ++FG++   + +TG+FAL
Sbjct:   54 VEEEVLQALLEATEEFELRYQRAFSDLTSQLHI-TQDTAQQSFQQVMGELFRDGTNWGRIVAFFSFGRALCVESANKEMTDLLPRIVQWMVNYLEHTLQPWMQENGGWEAFVGL-YGKNAAAQSRESQERFGRLLTIVMLTGVFAL 197
[Close]
BLAST of bcl2-like protein vs. UniProt/SwissProt
Match: sp|Q07816|B2CL1_CHICK (Bcl-2-like protein 1 OS=Gallus gallus OX=9031 GN=BCL2L1 PE=2 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 1.116e-6
Identity = 37/130 (28.46%), Postives = 60/130 (46.15%), Query Frame = 1 
Query:  268 DIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMY-----IIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGK 642
            D+ Q L     E + R +   S+ +S L+  T     QSF  VV  LF+D + W R++  F +     +  V K  +    +   W    +   + PW++ENGGWE+ V +  N N   E+ +  + F K
Sbjct:   81 DVRQALRDAGDEFELRYRRAFSDLTSQLHI-TPGTAYQSFEQVVNELFHDGVNWGRIVAFFSFGGALCVESVDKEMRVLVGRIVSWMTTYLTDHLDPWIQENGGWERFVDLYGN-NAAAELRKGQETFNK 208
[Close]
BLAST of bcl2-like protein vs. TrEMBL
Match: C9WPR4 (Bcl2-like protein OS=Schmidtea mediterranea OX=79327 GN=bcl2-1 PE=2 SV=1)

HSP 1 Score: 378.637 bits (971), Expect = 4.492e-127
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 1 
Query:  142 MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGKWIFN 693
            MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGKWIFN
Sbjct:    1 MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGKWIFN 184
[Close]
BLAST of bcl2-like protein vs. TrEMBL
Match: Q90ZH2 (Bcl-XL OS=Xenopus laevis OX=8355 GN=bcl2l1 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 5.926e-8
Identity = 41/146 (28.08%), Postives = 75/146 (51.37%), Query Frame = 1 
Query:  259 LNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNE-----YKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVA--LGITGLFAL 675
            + ++++Q L     E + R +   S+ +S L+  T D  +QSF  V+  LF D   W R++  F +   +   S N+       +  +W V  +   + PW++ENGGWE  V +   +N   +  E+ ++FG++   + +TG+FAL
Sbjct:   54 VEEEVLQALLEATEEFELRYQRAFSDLTSQLHI-TQDTAQQSFQQVMGELFRDGTNWGRIVAFFSFGRALCVESANKEMTDLLPRIVQWMVNYLEHTLQPWMQENGGWEAFVGL-YGKNAAAQSRESQERFGRLLTIVMLTGVFAL 197
[Close]
BLAST of bcl2-like protein vs. TrEMBL
Match: Q2TAP5 (Bcl-XL protein OS=Xenopus laevis OX=8355 GN=bcl2l1 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.883e-8
Identity = 41/146 (28.08%), Postives = 75/146 (51.37%), Query Frame = 1 
Query:  259 LNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNE-----YKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVA--LGITGLFAL 675
            + ++++Q L     E + R +   S+ +S L+  T D  +QSF  V+  LF D   W R++  F +   +   S N+       +  +W V  +   + PW++ENGGWE  V +   +N   +  E+ ++FG++   + +TG+FAL
Sbjct:   54 VEEEVLQALLEATEEFELRYQRAFSDLTSQLHI-TQDTAQQSFQQVMGELFRDGTNWGRIVAFFSFGRALCVESANKEMTDLLPRIVQWMVNYLEHTLQPWMQENGGWEAFVGL-YGKNAAAQSRESQERFGRLLTIVILTGVFAL 197
[Close]
BLAST of bcl2-like protein vs. TrEMBL
Match: R4TE41 (Bcl-2 like 1 protein OS=Ruditapes philippinarum OX=129788 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.602e-6
Identity = 44/168 (26.19%), Postives = 83/168 (49.40%), Query Frame = 1 
Query:  214 VKLAGIDQQLND-RNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYI-VVESLFNDCIRWSRVIVAFMYI--IQVLKISKNEYKKY---FEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVAL-GITGLFALGKWIFN 693
            V+ AG D Q++  RNC N+ ++  +  L  E + R +   S F+ +    +  +   + Y+ V++ L  + + W RV+  F +   + V  + KN+ ++     EW+ + + +K+  W+ EN GW  ++      N +++  E    +G V + G  G FALG  I N
Sbjct:   24 VQQAGFDYQVSPTRNCPNNKVIDTVKKLCSEFEKRYE---SQFTEMCETCSQVELNSANYVAVLDQLIVNGLHWGRVVAIFSFAGAMSVYCMKKNQKERVDWIREWTCQFMDSKVENWINENNGWRGLIDFYT--NGIKKPEEKTNGWGSVLIGGALGAFALGALIIN 186
[Close]
BLAST of bcl2-like protein vs. Ensembl Cavefish
Match: bcl2l1 (BCL2 like 1 [Source:ZFIN;Acc:ZDB-GENE-010730-1])

HSP 1 Score: 47.7506 bits (112), Expect = 9.913e-6
Identity = 34/120 (28.33%), Postives = 52/120 (43.33%), Query Frame = 1 
Query:  265 DDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMY-----IIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLE 609
            D + + L     E + R     S+ SS L+  T     QSF  V++ +F D + W RV+  F +     +  V K       +  EW    +   I PW++E GGWE+  +I  N    E
Sbjct:   88 DAVKEALRDSANEFELRYSRAFSDLSSQLHI-TPATAYQSFESVMDEVFRDGVNWGRVVGLFAFGGALCVECVEKEMSPLVGRIAEWMTVYLDNHIQPWIQEQGGWERFAEIFGNDTAAE 206
[Close]
BLAST of bcl2-like protein vs. Planmine SMEST
Match: SMESG000049099.1 (SMESG000049099.1)

HSP 1 Score: 378.637 bits (971), Expect = 1.249e-130
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 1 
Query:  142 MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGKWIFN 693
            MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGKWIFN
Sbjct:    1 MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGKWIFN 184
[Close]
BLAST of bcl2-like protein vs. Planmine SMEST
Match: SMESG000050542.1 (SMESG000050542.1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.725e-18
Identity = 49/131 (37.40%), Postives = 76/131 (58.02%), Query Frame = 1 
Query:  184 DYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQI 576
            DYI +++NGLV  +            +D I+QCL+ ++ E  D   D   +FS +  + TD DK+++F  V E+LF D I WS VI++ MYI  ++ +  N  +KY  WS +  L K+YPW+ ENG W+ +
Sbjct:    3 DYIITIVNGLVYGS------------DDVIIQCLNKIIKEI-DSECDI--DFSPMFLQETDKDKEKTFKDVFETLFEDGICWSNVIISLMYIRTIIILCPNYQQKYLNWSAQE-LIKVYPWITENGDWKYV 117
[Close]
BLAST of bcl2-like protein vs. Planmine SMEST
Match: SMESG000004697.1 (SMESG000004697.1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.875e-17
Identity = 46/139 (33.09%), Postives = 76/139 (54.68%), Query Frame = 1 
Query:  265 DDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKNRNHLEEVTETMKQFGKVALGITGLFALGK 681
            DDI++ +  +V   +   +D   ++  ILN  T + KK SF  +   +F++  +W R+IV  MY+  ++  S ++Y+ YF+W +E+I TKI PWV  NGGW  I+Q+S      +   E  K   K+  G + L+   K
Sbjct:  812 DDIIKNMKFMVNRTEKIVEDIPFDYEKILNLTTLEKKKNSFQSIFSYMFDENNKWMRIIVELMYMKHLILKSGSDYENYFQWFIEVIETKIDPWVETNGGWSAIIQVSAEAKRSDIYIENCKFLLKLLTGASVLYLCNK 950
[Close]
BLAST of bcl2-like protein vs. Planmine SMEST
Match: SMESG000079150.1 (SMESG000079150.1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.541e-16
Identity = 41/104 (39.42%), Postives = 65/104 (62.50%), Query Frame = 1 
Query:  265 DDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQI 576
            D ++  L  LV EA    +    +FS IL++ T + K+ +F  V  +L  + ++WSRVIVAFMY+  +L+ S + Y++Y  WS+++I  +I PWV ENG W+ I
Sbjct:   19 DQLISNLQFLVDEALKVYRRNPIDFSWILSRSTLNAKENAFKGVFYALLEN-MKWSRVIVAFMYMKIILQRSGDYYERYLYWSLQIIQNEISPWVAENGSWDNI 121
[Close]
BLAST of bcl2-like protein vs. Planmine SMEST
Match: SMESG000038292.1 (SMESG000038292.1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.454e-14
Identity = 47/133 (35.34%), Postives = 72/133 (54.14%), Query Frame = 1 
Query:  196 SLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHSLVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVIVAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISKN 594
            SLLN LV            N   DDI++ +  +V    +  +D    F  ILN  T + KK SF  +   +F+  I+W R+I+  MY+  ++  S ++Y+  F+WS+E+I TKI PWV ENG  EQ+  ++ N
Sbjct:    9 SLLNELVA----------NNGEEDDIIKNIKFMVNHTDNIVEDI--PFEPILNLSTLERKKNSFQSIFSYMFDKNIKWMRIIIGLMYMKHMILKSGSDYENDFQWSIEVIETKIDPWVEENGDGEQLFNLNDN 129
The following BLAST results are available for this feature:
BLAST of bcl2-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
BCL2L15.911e-625.52BCL2 like 1 [Source:HGNC Symbol;Acc:HGNC:992][more]
BCL2L15.911e-625.52BCL2 like 1 [Source:HGNC Symbol;Acc:HGNC:992][more]
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BLAST of bcl2-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
bcl2b1.654e-727.78BCL2 apoptosis regulator b [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of bcl2-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Bcl2l15.970e-625.52BCL2-like 1 [Source:MGI Symbol;Acc:MGI:88139][more]
Bcl2l15.970e-625.52BCL2-like 1 [Source:MGI Symbol;Acc:MGI:88139][more]
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BLAST of bcl2-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q91828|AR11_XENLA1.962e-1028.08Apoptosis regulator R11 OS=Xenopus laevis OX=8355 ... [more]
sp|Q07816|B2CL1_CHICK1.116e-628.46Bcl-2-like protein 1 OS=Gallus gallus OX=9031 GN=B... [more]
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BLAST of bcl2-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
C9WPR44.492e-127100.00Bcl2-like protein OS=Schmidtea mediterranea OX=793... [more]
Q90ZH25.926e-828.08Bcl-XL OS=Xenopus laevis OX=8355 GN=bcl2l1 PE=2 SV... [more]
Q2TAP59.883e-828.08Bcl-XL protein OS=Xenopus laevis OX=8355 GN=bcl2l1... [more]
R4TE413.602e-626.19Bcl-2 like 1 protein OS=Ruditapes philippinarum OX... [more]
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BLAST of bcl2-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
bcl2l19.913e-628.33BCL2 like 1 [Source:ZFIN;Acc:ZDB-GENE-010730-1][more]
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BLAST of bcl2-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bcl2-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000049099.11.249e-130100.00SMESG000049099.1[more]
SMESG000050542.13.725e-1837.40SMESG000050542.1[more]
SMESG000004697.12.875e-1733.09SMESG000004697.1[more]
SMESG000079150.15.541e-1639.42SMESG000079150.1[more]
SMESG000038292.13.454e-1435.34SMESG000038292.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30030127
GenBankACW83059.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030127 ID=SMED30030127|Name=bcl2-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1636bp
TTAATGTTTTGTTTTAATATAATAAATGTTTCCTCTTTTTAAATAACATT
TAAATTTATTTTAATTAAAGAAAGGTTTATACATTGTAGAATTTGTTGAT
ATTTCTGTGGTAGATATTTATCAATTAAACCGTAGCGTAGAATGTCTTAT
GTTAAAACTATGACACTAAATTATGATGATAAAGATTACATTCATTCTTT
GCTCAACGGTCTTGTTAAATTAGCAGGCATAGATCAACAGCTTAATGATC
GAAACTGTCTAAATGATGATATAGTACAATGCTTGCACAGTTTGGTGGTA
GAAGCTCAAGACCGTGGTAAAGATACAATATCTAATTTTTCATCGATTTT
AAATAAACCTACGGATGATGATAAAAAACAGTCGTTTTATATTGTGGTGG
AATCTTTATTTAACGATTGTATACGGTGGTCCAGAGTAATAGTAGCTTTT
ATGTATATAATTCAAGTTCTCAAAATTTCGAAAAATGAATACAAAAAATA
TTTTGAGTGGTCAGTTGAGTTGATCCTAACCAAAATATATCCATGGGTCA
GAGAAAATGGAGGATGGGAGCAAATAGTACAGATATCGAAAAACCGAAAC
CACCTCGAAGAAGTCACAGAAACAATGAAACAATTCGGCAAAGTGGCCCT
GGGGATAACTGGTTTGTTTGCATTGGGAAAATGGATATTCAATTGATGTT
AACAATATATATTGCTCAATTAATAACAAATTTATTTTTTTAATTTCCTT
CATGTGCCATGACGCTGCTCGCCGGTATTTATTGATTGGTTGCTAATTGA
TTGGAATGAATAGATTGATTGATTTTATGAAATGAATTGGTTGGAATGAA
TTGATTGATTTATTGATTGGAATGGATTGATTGATTTTATGGAATGAATT
GATTGATTGGTAGTGTGTGTGTGTGTAATGATTATCAATTAGGGGAGGGG
TGGATAGATTTATATTTGTTCTGGTGCTGAATGAATAATTGATAATTCCC
CTGCAGTGAGCACATGCTAGATGTGTGAGTGTGAGGCAGTGCAATTAACC
GATTTGCATAGTTACCAACTGACTGGTCATGGTCATGGCAGCTGCAAACT
GATCGATTTCACCCTCTTGGTCAATATATCTAACTCATCATTTTACCGTT
ATCGATTAAATAATTTTATTGAATCACTTAAATTTTTATTTTTATCGATT
TTCATTAAATTATAATTTATTGATTAATTTATGGGTAATTATAATAATCA
ATATTTTCAAGTCGCGGTTGTCGTAGGAGAACAACGAATCACATTTAGAC
CTATTATTAATTGATCTCAATTGATTATTTGTGTGGAAATCGATCAACCA
ATCGTATCGTGAGAATAGTAATAGTAATTATCGATCCAGGATCCGTTATT
GCCATTGATTCATTGACCTCGTTTCCCGACAGACACGTTGATATTTGTCT
GCATATGGTTTGTGTTATCGATCATTTGAATCTTTCACAGAATATTAATC
AATCAAAACAACCTTTAGAAATTGCAATCGATTTGGTATTGATTTTCACT
TTTCTGTCATATCAACGCGACTTATTTTTAAATTCGTTTAATAATTGATC
CTGTTACTTTATTTATCTTTTGTTTGCGAATCGAAC
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protein sequence of SMED30030127-orf-1

>SMED30030127-orf-1 ID=SMED30030127-orf-1|Name=SMED30030127-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=185bp
MSYVKTMTLNYDDKDYIHSLLNGLVKLAGIDQQLNDRNCLNDDIVQCLHS
LVVEAQDRGKDTISNFSSILNKPTDDDKKQSFYIVVESLFNDCIRWSRVI
VAFMYIIQVLKISKNEYKKYFEWSVELILTKIYPWVRENGGWEQIVQISK
NRNHLEEVTETMKQFGKVALGITGLFALGKWIFN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000070intestinal phagocyte
PLANA:0000136whole organism
PLANA:0002056medial muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036834Blc2-like_sf
IPR026298Blc2_fam
IPR002475Bcl2-like
Vocabulary: biological process
TermDefinition
GO:0042981regulation of apoptotic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036834Blc2-like superfamilyGENE3DG3DSA:1.10.437.10coord: 13..148
e-value: 7.1E-20
score: 73.4
IPR036834Blc2-like superfamilySUPERFAMILYSSF56854Bcl-2 inhibitors of programmed cell deathcoord: 36..178
IPR026298Blc2 familyPFAMPF00452Bcl-2coord: 53..142
e-value: 1.4E-9
score: 38.5
IPR026298Blc2 familyPANTHERPTHR11256BCL-2 RELATEDcoord: 35..174
IPR002475Bcl2-likePROSITEPS50062BCL2_FAMILYcoord: 48..144
score: 12.765