SMED30030118

Overview


Name	SMED30030118
Smed ID	SMED30030118
Length (bp)	622

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30030118 (SMED30030118) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30030118 (SMED30030118) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30030118

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
gut	SMED30030118	SMESG000072556.1	dd_Smed_v4_10620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30030118	SMESG000072556.1	dd_Smed_v4_10620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30030118	SMESG000072556.1	dd_Smed_v4_10620_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30030118 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.013567:991..7462 -	3020

Homology

[Close]

BLAST of SMED30030118 vs. Ensembl Human
Match: ANKS1A (ankyrin repeat and sterile alpha motif domain containing 1A [Source:HGNC Symbol;Acc:HGNC:20961])

HSP 1 Score: 54.299 bits (129), Expect = 3.734e-8
Identity = 41/146 (28.08%), Postives = 63/146 (43.15%), Query Frame = -1

Query:   59 IVQYHMDNETKHFKIHESLLIETTDVKKFKPSTPNPSFEEIILRIKNPEVSDIEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 496
            IV+  +     H +++E   +E  ++KK+ P  P                            +  K+  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LLN  P L+   N K  TPL LAA+
Sbjct:  128 IVRLLIHQGPSHTRVNEQNALEIKELKKYGPFDPY---------------------------INAKNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSC-NTKKHTPLHLAAR 245

[Close]

BLAST of SMED30030118 vs. Ensembl Human
Match: ANKS1A (ankyrin repeat and sterile alpha motif domain containing 1A [Source:HGNC Symbol;Acc:HGNC:20961])

HSP 1 Score: 53.5286 bits (127), Expect = 7.348e-8
Identity = 32/87 (36.78%), Postives = 47/87 (54.02%), Query Frame = -1

Query:   59 NNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 319
            +  V +++  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LLN  P L+   N K  TPL LAA+
Sbjct:  139 HTRVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSC-NTKKHTPLHLAAR 224

[Close]

BLAST of SMED30030118 vs. Ensembl Human
Match: ANKRD46 (ankyrin repeat domain 46 [Source:HGNC Symbol;Acc:HGNC:27229])

HSP 1 Score: 51.9878 bits (123), Expect = 7.471e-8
Identity = 30/86 (34.88%), Postives = 47/86 (54.65%), Query Frame = -1

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N +  TPL+LA ++ + K
Sbjct:   41 RDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Human
Match: ANKRD46 (ankyrin repeat domain 46 [Source:HGNC Symbol;Acc:HGNC:27229])

HSP 1 Score: 52.373 bits (124), Expect = 8.866e-8
Identity = 34/99 (34.34%), Postives = 54/99 (54.55%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  + N+  +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N +  TPL+LA ++ + K
Sbjct:   32 LESGF--DPNI--RDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Human
Match: ANKS1A (ankyrin repeat and sterile alpha motif domain containing 1A [Source:HGNC Symbol;Acc:HGNC:20961])

HSP 1 Score: 52.7582 bits (125), Expect = 9.861e-8
Identity = 33/91 (36.26%), Postives = 47/91 (51.65%), Query Frame = -1

Query:   29 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 301
            K+  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LLN  P L+   N K  TPL LAA+     +  +L
Sbjct:  166 KNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSC-NTKKHTPLHLAARNGHKAVVQVL 255

[Close]

BLAST of SMED30030118 vs. Ensembl Fly
Match: dgo (gene:FBgn0086898 transcript:FBtr0088249)

HSP 1 Score: 48.9062 bits (115), Expect = 1.252e-6
Identity = 31/95 (32.63%), Postives = 47/95 (49.47%), Query Frame = -1

Query:   29 DKDQKNQ---TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 304
            D D +N    T LH A  YG     + LL   CDP   +++GDT LH      +    + LL    +L GIKN + + P+ +A +K+  ++  IL
Sbjct:  156 DPDVRNNYGDTPLHTACRYGHAGVSRILLSALCDPNKTNLNGDTALHITCAMGRRKLTRILLEADARL-GIKNAQGDCPMHIAIRKNYREIIEIL 249

[Close]

BLAST of SMED30030118 vs. Ensembl Fly
Match: dgo (gene:FBgn0086898 transcript:FBtr0088250)

HSP 1 Score: 48.9062 bits (115), Expect = 1.252e-6
Identity = 31/95 (32.63%), Postives = 47/95 (49.47%), Query Frame = -1

Query:   29 DKDQKNQ---TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 304
            D D +N    T LH A  YG     + LL   CDP   +++GDT LH      +    + LL    +L GIKN + + P+ +A +K+  ++  IL
Sbjct:  156 DPDVRNNYGDTPLHTACRYGHAGVSRILLSALCDPNKTNLNGDTALHITCAMGRRKLTRILLEADARL-GIKNAQGDCPMHIAIRKNYREIIEIL 249

[Close]

BLAST of SMED30030118 vs. Ensembl Zebrafish
Match: ankrd46b (ankyrin repeat domain 46b [Source:NCBI gene;Acc:402888])

HSP 1 Score: 51.9878 bits (123), Expect = 6.968e-8
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = -1

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   41 RDHRGRTGLHLAAARGNVDICRFLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHNGSTPLVLAKRRGVNK 122

[Close]

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   41 RDHRGRTGLHLAAARGNVDICRFLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHNGSTPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 52.373 bits (124), Expect = 1.057e-7
Identity = 32/86 (37.21%), Postives = 49/86 (56.98%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK---KDLTKLFL 283
            T LH A+F G ++ +K+LL+ G  P A ++  +T LH A  +   + A+FLL    Q +  K   D+TPL  AA+   K+L KL +
Sbjct:  374 TPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQ-VDAKAKDDQTPLHCAARMGHKELVKLLM 458

[Close]

BLAST of SMED30030118 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 52.373 bits (124), Expect = 1.067e-7
Identity = 32/86 (37.21%), Postives = 49/86 (56.98%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK---KDLTKLFL 283
            T LH A+F G ++ +K+LL+ G  P A ++  +T LH A  +   + A+FLL    Q +  K   D+TPL  AA+   K+L KL +
Sbjct:  347 TPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQ-VDAKAKDDQTPLHCAARMGHKELVKLLM 431

[Close]

BLAST of SMED30030118 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 52.373 bits (124), Expect = 1.077e-7
Identity = 32/86 (37.21%), Postives = 49/86 (56.98%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK---KDLTKLFL 283
            T LH A+F G ++ +K+LL+ G  P A ++  +T LH A  +   + A+FLL    Q +  K   D+TPL  AA+   K+L KL +
Sbjct:  379 TPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQ-VDAKAKDDQTPLHCAARMGHKELVKLLM 463

[Close]

BLAST of SMED30030118 vs. Ensembl Xenopus
Match: kcnk16 (potassium channel, two pore domain subfamily K, member 16 [Source:Xenbase;Acc:XB-GENE-996240])

HSP 1 Score: 52.7582 bits (125), Expect = 6.802e-8
Identity = 31/86 (36.05%), Postives = 47/86 (54.65%), Query Frame = -1

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N +  TPLILA ++ + K
Sbjct:   41 RDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLILAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 49.6766 bits (117), Expect = 1.296e-6
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKK---DLTKLFL 283
            T LH ASF G +  +KTLL+ G  P    +  +T LH A  +   D A FL+  K + +  +   D+TPL  AA+    ++ +L L
Sbjct:  397 TPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAK-VNARAKDDQTPLHCAARSGHYEMVRLLL 481

[Close]

BLAST of SMED30030118 vs. Ensembl Xenopus
Match: atp5f1c (ATP synthase F1 subunit gamma [Source:Xenbase;Acc:XB-GENE-970730])

HSP 1 Score: 49.2914 bits (116), Expect = 1.561e-6
Identity = 28/80 (35.00%), Postives = 42/80 (52.50%), Query Frame = -1

Query:   62 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAA 301
            +D +    LH A+F G+V   + LL   CD  AV+I GD+ LH A    + +     L    + +G+KN + ETPL  +A
Sbjct:  772 RDNEENICLHWAAFAGSVEIAEILLASKCDLRAVNIHGDSPLHIAARENRHECVMLFLGHGSE-VGLKNKEGETPLECSA 850

[Close]

BLAST of SMED30030118 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 49.2914 bits (116), Expect = 1.639e-6
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKK---DLTKLFL 283
            T LH ASF G +  +KTLL+ G  P    +  +T LH A  +   D A FL+  K + +  +   D+TPL  AA+    ++ +L L
Sbjct:  402 TPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAK-VNARAKDDQTPLHCAARSGHYEMVRLLL 486

[Close]

BLAST of SMED30030118 vs. Ensembl Xenopus
Match: ANK1 (ankyrin 1 [Source:NCBI gene;Acc:613080])

HSP 1 Score: 49.2914 bits (116), Expect = 1.654e-6
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKK---DLTKLFL 283
            T LH ASF G +  +KTLL+ G  P    +  +T LH A  +   D A FL+  K + +  +   D+TPL  AA+    ++ +L L
Sbjct:  364 TPLHVASFMGHLPIVKTLLQKGASPNVFSVKVETPLHMAARAAHTDVADFLIQNKAK-VNARAKDDQTPLHCAARSGHYEMVRLLL 448

[Close]

BLAST of SMED30030118 vs. Ensembl Mouse
Match: Ankrd46 (ankyrin repeat domain 46 [Source:MGI Symbol;Acc:MGI:1916089])

HSP 1 Score: 56.225 bits (134), Expect = 2.397e-9
Identity = 35/99 (35.35%), Postives = 55/99 (55.56%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  + N+  +D + +T LH A+  G V   + L K G DPLA D  G+T LH   +   +D  +FL++   + I I N +  TPL+LA ++ + K
Sbjct:   32 LESGF--DPNI--RDSRGRTGLHLAAARGNVDICQLLHKFGADPLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Mouse
Match: Anks1 (ankyrin repeat and SAM domain containing 1 [Source:MGI Symbol;Acc:MGI:2446180])

HSP 1 Score: 50.0618 bits (118), Expect = 7.111e-7
Identity = 39/146 (26.71%), Postives = 61/146 (41.78%), Query Frame = -1

Query:   59 IVQYHMDNETKHFKIHESLLIETTDVKKFKPSTPNPSFEEIILRIKNPEVSDIEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 496
            IV+  +     H +++E   +E  ++KK+ P  P                            +  K+  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LL   P L+     K  TPL LAA+
Sbjct:  124 IVRLLIQQGPSHTRVNEQNALEIRELKKYGPFDPY---------------------------INAKNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLGAHPNLLSCSTRK-HTPLHLAAR 241

[Close]

BLAST of SMED30030118 vs. Ensembl Mouse
Match: Anks1 (ankyrin repeat and SAM domain containing 1 [Source:MGI Symbol;Acc:MGI:2446180])

HSP 1 Score: 50.0618 bits (118), Expect = 7.352e-7
Identity = 39/146 (26.71%), Postives = 61/146 (41.78%), Query Frame = -1

Query:   59 IVQYHMDNETKHFKIHESLLIETTDVKKFKPSTPNPSFEEIILRIKNPEVSDIEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 496
            IV+  +     H +++E   +E  ++KK+ P  P                            +  K+  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LL   P L+     K  TPL LAA+
Sbjct:  124 IVRLLIQQGPSHTRVNEQNALEIRELKKYGPFDPY---------------------------INAKNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLGAHPNLLSCSTRK-HTPLHLAAR 241

[Close]

BLAST of SMED30030118 vs. Ensembl Mouse
Match: Anks1 (ankyrin repeat and SAM domain containing 1 [Source:MGI Symbol;Acc:MGI:2446180])

HSP 1 Score: 48.9062 bits (115), Expect = 1.685e-6
Identity = 30/87 (34.48%), Postives = 45/87 (51.72%), Query Frame = -1

Query:   59 NNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 319
            +  V +++  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LL   P L+     K  TPL LAA+
Sbjct:  135 HTRVNEQNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLGAHPNLLSCSTRK-HTPLHLAAR 220

[Close]

BLAST of SMED30030118 vs. Ensembl Mouse
Match: Anks1 (ankyrin repeat and SAM domain containing 1 [Source:MGI Symbol;Acc:MGI:2446180])

HSP 1 Score: 48.9062 bits (115), Expect = 1.710e-6
Identity = 30/87 (34.48%), Postives = 45/87 (51.72%), Query Frame = -1

Query:   59 NNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 319
            +  V +++  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LL   P L+     K  TPL LAA+
Sbjct:  135 HTRVNEQNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLGAHPNLLSCSTRK-HTPLHLAAR 220

[Close]

BLAST of SMED30030118 vs. UniProt/SwissProt
Match: sp|Q8BTZ5|ANR46_MOUSE (Ankyrin repeat domain-containing protein 46 OS=Mus musculus OX=10090 GN=Ankrd46 PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.677e-8
Identity = 35/99 (35.35%), Postives = 55/99 (55.56%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  + N+  +D + +T LH A+  G V   + L K G DPLA D  G+T LH   +   +D  +FL++   + I I N +  TPL+LA ++ + K
Sbjct:   32 LESGF--DPNI--RDSRGRTGLHLAAARGNVDICQLLHKFGADPLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. UniProt/SwissProt
Match: sp|Q76K24|ANR46_RAT (Ankyrin repeat domain-containing protein 46 OS=Rattus norvegicus OX=10116 GN=Ankrd46 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.761e-8
Identity = 35/99 (35.35%), Postives = 55/99 (55.56%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  + N+  +D + +T LH A+  G V   + L K G DPLA D  G+T LH   +   +D  +FL++   + I I N +  TPL+LA ++ + K
Sbjct:   32 LESGF--DPNI--RDSRGRTGLHLAAARGNVDICQLLHKFGADPLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. UniProt/SwissProt
Match: sp|Q02989|LITA_LATTR (Alpha-latroinsectotoxin-Lt1a (Fragment) OS=Latrodectus tredecimguttatus OX=6925 PE=1 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 2.395e-7
Identity = 34/124 (27.42%), Postives = 57/124 (45.97%), Query Frame = -1

Query:   38 ILRIKNPEVSDIEEGFVGNN------------NVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLF 373
            + R+ +   S +  G +G N            NV DK     T LH A+  G  +  + LL  G D  A DI+    +H A  +  ++  + L+ + P L+ +KN+++E P  LA +K    +F
Sbjct:  763 VTRLTSKGFSALHLGIIGKNEEIPFFLVEKGANVNDKTNSGVTPLHFAAGLGKANIFRLLLSRGADIKAEDINSQMPIHEAVSNGHLEIVRILIEKDPSLMNVKNIRNEYPFYLAVEKRYKDIF 886

[Close]

BLAST of SMED30030118 vs. UniProt/SwissProt
Match: sp|Q92625|ANS1A_HUMAN (Ankyrin repeat and SAM domain-containing protein 1A OS=Homo sapiens OX=9606 GN=ANKS1A PE=1 SV=4)

HSP 1 Score: 53.5286 bits (127), Expect = 3.529e-7
Identity = 32/87 (36.78%), Postives = 47/87 (54.02%), Query Frame = -1

Query:   59 NNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 319
            +  V +++  N+TALHCA+ YG    +K LL+   DP   +   +T L  A    +++  K LLN  P L+   N K  TPL LAA+
Sbjct:  139 HTRVNEQNNDNETALHCAAQYGHTEVVKVLLEELTDPTMRNNKFETPLDLAALYGRLEVVKMLLNAHPNLLSC-NTKKHTPLHLAAR 224

[Close]

BLAST of SMED30030118 vs. UniProt/SwissProt
Match: sp|Q86W74|ANR46_HUMAN (Ankyrin repeat domain-containing protein 46 OS=Homo sapiens OX=9606 GN=ANKRD46 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 4.258e-7
Identity = 34/99 (34.34%), Postives = 54/99 (54.55%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  + N+  +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N +  TPL+LA ++ + K
Sbjct:   32 LESGF--DPNI--RDSRGRTGLHLAAARGNVDICQLLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHQGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. TrEMBL
Match: A0A179FKU8 (Ankyrin OS=Pochonia chlamydosporia 170 OX=1380566 GN=VFPPC_07553 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.138e-9
Identity = 39/102 (38.24%), Postives = 57/102 (55.88%), Query Frame = -1

Query:   20 GNNNVL-DKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWNY 322
            GNN +L  +D + QTALH A+  G  S I+ LL  G +P   D+ GDT LH A Y +++   + LL  K   +  KN    + L LAA K  T +F++L ++
Sbjct:  402 GNNEILQSRDHEGQTALHNAAEQGYESVIELLLDHGANPTIKDLSGDTALHLAAYHRQLGAVQALLKAKSIDVDAKNDNGRSALFLAAAKGHTDIFILLLSH 503

[Close]

BLAST of SMED30030118 vs. TrEMBL
Match: A0A4Q7JY08 (ANK_REP_REGION domain-containing protein OS=Pochonia chlamydosporia 123 OX=1052797 GN=I1G_00002259 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 5.568e-9
Identity = 38/102 (37.25%), Postives = 57/102 (55.88%), Query Frame = -1

Query:   20 GNNNVL-DKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWNY 322
            GNN +L  +D + QTALH A+  G  S I+ LL  G +P   D+ GDT LH A Y +++   + LL  +   +  KN    + L LAA K  T +F++L ++
Sbjct:  362 GNNEILQSRDHEGQTALHNAAEQGYESVIELLLDHGANPTIKDLSGDTALHLAAYHRQLGAVQALLKAESIDVDAKNDNGRSALFLAAAKGHTDIFILLLSH 463

[Close]

BLAST of SMED30030118 vs. TrEMBL
Match: A0A2N1TNB4 (Uncharacterized protein OS=Spirochaetae bacterium HGW-Spirochaetae-1 OX=2013832 GN=CVV44_03445 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.461e-8
Identity = 35/95 (36.84%), Postives = 54/95 (56.84%), Query Frame = -1

Query:   29 NVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 313
            NV +   ++QT LH A+F G +S ++ LL+ G D  A D+DG T LHFA ++ K   A+ L+      +  KN    TPL  AA +D  ++ ++L
Sbjct:  197 NVNEHSMRDQTPLHFAAFEGDISIVRVLLESGADINARDMDGMTALHFAAFNGKTAVARILVENGAD-VNAKNENGGTPLHHAAYQDSGEIAIML 290

[Close]

BLAST of SMED30030118 vs. TrEMBL
Match: A0A1V0S8A7 (SWPV1-303 OS=Shearwaterpox virus OX=1974596 GN=SWPV1-303 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.243e-7
Identity = 41/94 (43.62%), Postives = 52/94 (55.32%), Query Frame = -1

Query:   35 DKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK----KDLTKLFL 304
            DK+ K +T LH AS Y     IK LL  G D  ++DI G T LH AT S  +D A+FL+N   + I  K+  DETPL  A K      +TKL +
Sbjct:   43 DKNLKGKTPLHYASKYSNCDIIKLLLDNGADIHSLDIKGKTPLHCATESSFVDNAEFLINNGAE-INAKDFNDETPLHYATKIFFGNSVTKLLI 135

[Close]

BLAST of SMED30030118 vs. TrEMBL
Match: G0V187 (Uncharacterized protein TCIL3000_11_8620 OS=Trypanosoma congolense (strain IL3000) OX=1068625 GN=TCIL3000_11_8620 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.564e-7
Identity = 32/100 (32.00%), Postives = 57/100 (57.00%), Query Frame = -1

Query:    2 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWNYDKTLIN 301
            +D++ +TALH A+  G +  ++ LL  G  P A + +G+T LHFA  + +++ A+ LL +       +N  D+TP+ L   K   ++ L+L  +D+ L N
Sbjct:   47 QDEQGRTALHAAAANGRMDMLEILLSYGPTPDATNNEGNTALHFAALNNQVEAARLLL-KNGWRASARNTYDKTPIQLIYGKQFEEMELLLLGFDEELDN 145

[Close]

BLAST of SMED30030118 vs. Ensembl Cavefish
Match: ankrd46b (ankyrin repeat domain-containing protein 46 [Source:NCBI gene;Acc:103025974])

HSP 1 Score: 51.6026 bits (122), Expect = 8.129e-8
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = -1

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   41 RDNRGRTGLHLAAARGNVEICRFLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHNGSTPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Cavefish
Match: ankrd46a (ankyrin repeat domain-containing protein 46-like [Source:NCBI gene;Acc:103024951])

HSP 1 Score: 51.2174 bits (121), Expect = 9.583e-8
Identity = 34/99 (34.34%), Postives = 51/99 (51.52%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  N     +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   32 LESGFDPNA----RDCRGRTGLHLAAARGNVDICRLLHKFGADLLATDAQGNTALHMCGH---VDTIQFLVSNGLK-IDICNHNGATPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 52.373 bits (124), Expect = 9.995e-8
Identity = 33/86 (38.37%), Postives = 49/86 (56.98%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK---KDLTKLFL 283
            T LH A+F G ++ +K+LL+ G  P A ++  +T LH A  +   + A+FLL    Q +  K   D+TPL  AA+   K+L KL L
Sbjct:  381 TPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQ-VDAKAKDDQTPLHCAARMGHKELVKLLL 465

[Close]

BLAST of SMED30030118 vs. Ensembl Cavefish
Match: ankrd46a (ankyrin repeat domain-containing protein 46-like [Source:NCBI gene;Acc:103024951])

HSP 1 Score: 51.2174 bits (121), Expect = 1.207e-7
Identity = 34/99 (34.34%), Postives = 51/99 (51.52%), Query Frame = -1

Query:   44 IEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 340
            +E GF  N     +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   57 LESGFDPNA----RDCRGRTGLHLAAARGNVDICRLLHKFGADLLATDAQGNTALHMCGH---VDTIQFLVSNGLK-IDICNHNGATPLVLAKRRGVNK 147

[Close]

BLAST of SMED30030118 vs. Ensembl Cavefish
Match: ankrd46b (ankyrin repeat domain-containing protein 46 [Source:NCBI gene;Acc:103025974])

HSP 1 Score: 51.2174 bits (121), Expect = 1.228e-7
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = -1

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   41 RDNRGRTGLHLAAARGNVEICRFLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHNGSTPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 7.349e-8
Identity = 29/86 (33.72%), Postives = 52/86 (60.47%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAA---KKDLTKLFL 283
            T +H ASF G ++ ++ LL+ G    A ++ G+T LH A+ + +++  K LL Q    +G+K  +D+TPL +A+   K ++ +L L
Sbjct:  405 TPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLL-QSGARVGVKAREDQTPLHIASRLGKAEIVQLLL 489

[Close]

BLAST of SMED30030118 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 8.004e-8
Identity = 29/86 (33.72%), Postives = 52/86 (60.47%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAA---KKDLTKLFL 283
            T +H ASF G ++ ++ LL+ G    A ++ G+T LH A+ + +++  K LL Q    +G+K  +D+TPL +A+   K ++ +L L
Sbjct:  412 TPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLL-QSGARVGVKAREDQTPLHIASRLGKAEIVQLLL 496

[Close]

BLAST of SMED30030118 vs. Ensembl Sea Lamprey
Match: anks1b (ankyrin repeat and sterile alpha motif domain containing 1B [Source:NCBI gene;Acc:565408])

HSP 1 Score: 49.6766 bits (117), Expect = 1.546e-7
Identity = 30/91 (32.97%), Postives = 45/91 (49.45%), Query Frame = -1

Query:   29 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 301
            ++  N+TALHCA+ YG  + ++ LL+   DP   +   +T L  A    ++D    L    P L+   N K  TPL LAA+   T    +L
Sbjct:  122 QNHDNETALHCAAQYGHTAVVRLLLEELTDPTMRNARFETPLDLAALYGRLDVVTLLTRSHPNLLAC-NTKQHTPLHLAARNGHTGTVQVL 211

[Close]

BLAST of SMED30030118 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008505.1 (pep scaffold:Pmarinus_7.0:GL477211:28706:49738:-1 gene:ENSPMAG00000007672.1 transcript:ENSPMAT00000008505.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.9802 bits (110), Expect = 1.069e-6
Identity = 33/110 (30.00%), Postives = 53/110 (48.18%), Query Frame = -1

Query:   56 KNPEVSDIEEGFVGNNNVL--------DKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKK 361
            K   +S+  +G VGN   L        +  Q+ +TALH A+  G +   + LLK G      D  G+T LH+A +  +   A  L++ + + +  +N    T L LAA+K
Sbjct:  420 KKQLISEAAQGNVGNVQKLVQLFPGQINAQQEGRTALHVAAHQGHIDVARALLKAGASTEIGDDKGNTALHYAAHGNEAAMAGLLVSHRAR-VNSRNHAGRTALHLAARK 528

[Close]

BLAST of SMED30030118 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.0542 bits (105), Expect = 5.365e-6
Identity = 23/75 (30.67%), Postives = 43/75 (57.33%), Query Frame = -1

Query:   59 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 283
            T +H ++F G ++ +  LL+ G  P A ++ G+T LH A  + + +  + LL    Q +  K  +++TPL +AA+
Sbjct:   50 TPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQ-VDAKAREEQTPLHIAAR 123

[Close]

BLAST of SMED30030118 vs. Ensembl Nematostella
Match: EDO39546 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9P8])

HSP 1 Score: 45.4394 bits (106), Expect = 7.863e-7
Identity = 23/82 (28.05%), Postives = 46/82 (56.10%), Query Frame = -1

Query:   59 DKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK 304
            D+D++ +T +H A+  G V  ++ L + G     VD+ G+T++H   +   +D  +FL++        +N+  +TPL+LA +
Sbjct:    4 DRDERGRTPMHMAAMRGNVEMLELLYQHGGKLGTVDLIGNTLMHMCNH---VDVLQFLVDHGLSATE-RNINGDTPLVLAMR 81

[Close]

BLAST of SMED30030118 vs. Ensembl Nematostella
Match: EDO48893 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHQ3])

HSP 1 Score: 43.5134 bits (101), Expect = 9.798e-6
Identity = 30/96 (31.25%), Postives = 48/96 (50.00%), Query Frame = -1

Query:   29 NVLDKDQKNQT-ALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 313
            ++++  Q NQ  ALH   F G +  ++ L+  G D    + +G T LH+   S   D AKFL+ +K     I +   +TP+      DL +LFL+ 
Sbjct:   38 SIINGTQGNQGPALHQLVFEGNLKCVRMLVNLGADVNMQNEEGFTPLHYCALSNNFDMAKFLM-KKSANPDIADYNGKTPVDYTEDFDLIELFLMF 132

[Close]

BLAST of SMED30030118 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 54.6842 bits (130), Expect = 1.737e-8
Identity = 35/86 (40.70%), Postives = 49/86 (56.98%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK---KDLTKLFL 283
            T LH ASF G ++ +K LL+ G  P A ++  +T LH A+ S   + A+FLL Q    +  K   D+TPL  AA+   K+L KL L
Sbjct:  344 TPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLL-QNAAPVDAKAKDDQTPLHCAARMGHKELVKLLL 428

[Close]

BLAST of SMED30030118 vs. Ensembl Medaka
Match: ankrd46b (ankyrin repeat domain 46 [Source:NCBI gene;Acc:101161514])

HSP 1 Score: 51.6026 bits (122), Expect = 8.237e-8
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = -1

Query:   44 KDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTK 301
            +D + +T LH A+  G V   + L K G D LA D  G+T LH   +   +D  +FL++   + I I N    TPL+LA ++ + K
Sbjct:   41 RDNRGRTGLHLAAARGNVDICRLLHKFGADLLATDYQGNTALHLCGH---VDTIQFLVSNGLK-IDICNHNGSTPLVLAKRRGVNK 122

[Close]

BLAST of SMED30030118 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 52.373 bits (124), Expect = 1.181e-7
Identity = 32/86 (37.21%), Postives = 49/86 (56.98%), Query Frame = -1

Query:   35 TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAK---KDLTKLFL 283
            T LH A+F G ++ +K LL+ G  P A ++  +T LH A+ +   + A+FLL    Q +  +   D+TPL  AA+   K+L KL L
Sbjct:  404 TPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQ-VDARAKDDQTPLHCAARMGHKELVKLLL 488

[Close]

BLAST of SMED30030118 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479])

HSP 1 Score: 47.7506 bits (112), Expect = 3.834e-6
Identity = 31/92 (33.70%), Postives = 47/92 (51.09%), Query Frame = -1

Query:   29 DQKNQ--TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 298
            D KNQ  TAL  A+  G +  +K LL+  C     D DGDT LH+A +  + + A+ LL++    + + N    T L +A  K  T +  +L
Sbjct:  507 DIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGAN-VNLLNNSMCTALHIAVNKGFTDVVRVL 597

[Close]

BLAST of SMED30030118 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479])

HSP 1 Score: 47.7506 bits (112), Expect = 3.943e-6
Identity = 31/92 (33.70%), Postives = 47/92 (51.09%), Query Frame = -1

Query:   29 DQKNQ--TALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLIL 298
            D KNQ  TAL  A+  G +  +K LL+  C     D DGDT LH+A +  + + A+ LL++    + + N    T L +A  K  T +  +L
Sbjct:  521 DIKNQGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGAN-VNLLNNSMCTALHIAVNKGFTDVVRVL 611

[Close]

BLAST of SMED30030118 vs. Planmine SMEST
Match: SMESG000005009.1 (SMESG000005009.1)

HSP 1 Score: 48.9062 bits (115), Expect = 9.662e-7
Identity = 27/83 (32.53%), Postives = 43/83 (51.81%), Query Frame = -1

Query:   59 DQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQ---LIGIKNLKDETPLILAAK 298
            D +  T +H A+F+G +  ++ LL+ G     VD  G+T  H A     +   KFL+ Q P    ++G +N   ETP +LA +
Sbjct:  135 DYQGWTPVHGAAFHGRLGCLQFLLRWGLRSDDVDNQGNTAAHLAAMEGHLPVLKFLVAQGPTSSCVLGCRNDDGETPKMLAQR 217

[Close]

BLAST of SMED30030118 vs. Planmine SMEST
Match: SMESG000051358.1 (SMESG000051358.1)

HSP 1 Score: 46.595 bits (109), Expect = 5.194e-6
Identity = 40/133 (30.08%), Postives = 60/133 (45.11%), Query Frame = -1

Query:    5 STPNPSFEEIILRIKNPEVSDIEEGFVGNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWNYDKTLI 403
            S  + +F+ I     N  V  IE       ++   D   +T L+ ++FYG +     LL  G D    D++G+  +HFA+ S   D  + LL      I IKN   ETP  LA +K   +L   L   ++ LI
Sbjct:   66 SENDENFQAIHFAAVNNHVKCIETLLEFGVSIESCDMYRRTPLNISAFYGRIIASMFLLSRGADLNHQDMNGNHSIHFASLSGNSDLCQILLYSGVD-IHIKNDFGETPFHLACRKGSKRLLKSLMRNERKLI 197

[Close]

BLAST of SMED30030118 vs. Planmine SMEST
Match: SMESG000016986.1 (SMESG000016986.1)

HSP 1 Score: 45.4394 bits (106), Expect = 7.775e-6
Identity = 25/97 (25.77%), Postives = 46/97 (47.42%), Query Frame = -1

Query:   23 VLDKDQKNQTALHCASFYGTVSKIKTLLKCG-CDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWN 310
            V D+D +  T LH A     ++ ++ +++C    P+    +G T LHF  +S   +  K L+  +P+LI ++ L   T  + A +     +   L N
Sbjct:   74 VTDQDPEGLTVLHVACLTANLNIVQWVIQCTHLSPVTRTEEGATALHFCCWSGNFEITKLLIETEPRLINMRMLNGATAAMFATQGGYIDIMEFLLN 170

[Close]

BLAST of SMED30030118 vs. Planmine SMEST
Match: SMESG000016986.1 (SMESG000016986.1)

HSP 1 Score: 45.4394 bits (106), Expect = 7.821e-6
Identity = 25/97 (25.77%), Postives = 46/97 (47.42%), Query Frame = -1

Query:   23 VLDKDQKNQTALHCASFYGTVSKIKTLLKCG-CDPLAVDIDGDTVLHFATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWN 310
            V D+D +  T LH A     ++ ++ +++C    P+    +G T LHF  +S   +  K L+  +P+LI ++ L   T  + A +     +   L N
Sbjct:   74 VTDQDPEGLTVLHVACLTANLNIVQWVIQCTHLSPVTRTEEGATALHFCCWSGNFEITKLLIETEPRLINMRMLNGATAAMFATQGGYIDIMEFLLN 170

The following BLAST results are available for this feature:

BLAST of SMED30030118 vs. Ensembl Human
BLAST of SMED30030118 vs. Ensembl Celegans
BLAST of SMED30030118 vs. Ensembl Fly
BLAST of SMED30030118 vs. Ensembl Zebrafish
BLAST of SMED30030118 vs. Ensembl Xenopus
BLAST of SMED30030118 vs. Ensembl Mouse
BLAST of SMED30030118 vs. UniProt/SwissProt
BLAST of SMED30030118 vs. TrEMBL
BLAST of SMED30030118 vs. Ensembl Cavefish
BLAST of SMED30030118 vs. Ensembl Sea Lamprey
BLAST of SMED30030118 vs. Ensembl Yeast
BLAST of SMED30030118 vs. Ensembl Nematostella
BLAST of SMED30030118 vs. Ensembl Medaka
BLAST of SMED30030118 vs. Planmine SMEST

BLAST of SMED30030118 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ANKS1A	3.734e-8	28.08	ankyrin repeat and sterile alpha motif domain cont...	[more]
ANKS1A	7.348e-8	36.78	ankyrin repeat and sterile alpha motif domain cont...	[more]
ANKRD46	7.471e-8	34.88	ankyrin repeat domain 46 [Source:HGNC Symbol;Acc:H...	[more]
ANKRD46	8.866e-8	34.34	ankyrin repeat domain 46 [Source:HGNC Symbol;Acc:H...	[more]
ANKS1A	9.861e-8	36.26	ankyrin repeat and sterile alpha motif domain cont...	[more]

BLAST of SMED30030118 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30030118 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dgo	1.252e-6	32.63	gene:FBgn0086898 transcript:FBtr0088249	[more]
dgo	1.252e-6	32.63	gene:FBgn0086898 transcript:FBtr0088250	[more]

BLAST of SMED30030118 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ankrd46b	6.968e-8	34.88	ankyrin repeat domain 46b [Source:NCBI gene;Acc:40...	[more]
ankrd46b	6.968e-8	34.88	ankyrin repeat domain 46b [Source:NCBI gene;Acc:40...	[more]
BX640476.1	1.057e-7	37.21	ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79...	[more]
ank1b	1.067e-7	37.21	ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN...	[more]
BX640476.1	1.077e-7	37.21	ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79...	[more]

BLAST of SMED30030118 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
kcnk16	6.802e-8	36.05	potassium channel, two pore domain subfamily K, me...	[more]
ANK1	1.296e-6	34.88	ankyrin 1 [Source:NCBI gene;Acc:613080]	[more]
atp5f1c	1.561e-6	35.00	ATP synthase F1 subunit gamma [Source:Xenbase;Acc:...	[more]
ANK1	1.639e-6	34.88	ankyrin 1 [Source:NCBI gene;Acc:613080]	[more]
ANK1	1.654e-6	34.88	ankyrin 1 [Source:NCBI gene;Acc:613080]	[more]

BLAST of SMED30030118 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ankrd46	2.397e-9	35.35	ankyrin repeat domain 46 [Source:MGI Symbol;Acc:MG...	[more]
Anks1	7.111e-7	26.71	ankyrin repeat and SAM domain containing 1 [Source...	[more]
Anks1	7.352e-7	26.71	ankyrin repeat and SAM domain containing 1 [Source...	[more]
Anks1	1.685e-6	34.48	ankyrin repeat and SAM domain containing 1 [Source...	[more]
Anks1	1.710e-6	34.48	ankyrin repeat and SAM domain containing 1 [Source...	[more]

BLAST of SMED30030118 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8BTZ5\|ANR46_MOUSE	1.677e-8	35.35	Ankyrin repeat domain-containing protein 46 OS=Mus...	[more]
sp\|Q76K24\|ANR46_RAT	1.761e-8	35.35	Ankyrin repeat domain-containing protein 46 OS=Rat...	[more]
sp\|Q02989\|LITA_LATTR	2.395e-7	27.42	Alpha-latroinsectotoxin-Lt1a (Fragment) OS=Latrode...	[more]
sp\|Q92625\|ANS1A_HUMAN	3.529e-7	36.78	Ankyrin repeat and SAM domain-containing protein 1...	[more]
sp\|Q86W74\|ANR46_HUMAN	4.258e-7	34.34	Ankyrin repeat domain-containing protein 46 OS=Hom...	[more]

BLAST of SMED30030118 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A179FKU8	1.138e-9	38.24	Ankyrin OS=Pochonia chlamydosporia 170 OX=1380566 ...	[more]
A0A4Q7JY08	5.568e-9	37.25	ANK_REP_REGION domain-containing protein OS=Pochon...	[more]
A0A2N1TNB4	2.461e-8	36.84	Uncharacterized protein OS=Spirochaetae bacterium ...	[more]
A0A1V0S8A7	1.243e-7	43.62	SWPV1-303 OS=Shearwaterpox virus OX=1974596 GN=SWP...	[more]
G0V187	1.564e-7	32.00	Uncharacterized protein TCIL3000_11_8620 OS=Trypan...	[more]

BLAST of SMED30030118 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ankrd46b	8.129e-8	34.88	ankyrin repeat domain-containing protein 46 [Sourc...	[more]
ankrd46a	9.583e-8	34.34	ankyrin repeat domain-containing protein 46-like [...	[more]
ank1b	9.995e-8	38.37	ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN...	[more]
ankrd46a	1.207e-7	34.34	ankyrin repeat domain-containing protein 46-like [...	[more]
ankrd46b	1.228e-7	34.88	ankyrin repeat domain-containing protein 46 [Sourc...	[more]

BLAST of SMED30030118 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000000095.1	7.349e-8	33.72	pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g...	[more]
ENSPMAT00000000093.1	8.004e-8	33.72	pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g...	[more]
anks1b	1.546e-7	32.97	ankyrin repeat and sterile alpha motif domain cont...	[more]
ENSPMAT00000008505.1	1.069e-6	30.00	pep scaffold:Pmarinus_7.0:GL477211:28706:49738:-1 ...	[more]
ENSPMAT00000007449.1	5.365e-6	30.67	pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1...	[more]

BLAST of SMED30030118 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30030118 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO39546	7.863e-7	28.05	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO48893	9.798e-6	31.25	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30030118 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ank1a	1.737e-8	40.70	ankyrin-1 [Source:NCBI gene;Acc:101174837]	[more]
ankrd46b	8.237e-8	34.88	ankyrin repeat domain 46 [Source:NCBI gene;Acc:101...	[more]
ank1b	1.181e-7	37.21	ankyrin-1 [Source:NCBI gene;Acc:101157380]	[more]
mib2	3.834e-6	33.70	mindbomb E3 ubiquitin protein ligase 2 [Source:NCB...	[more]
mib2	3.943e-6	33.70	mindbomb E3 ubiquitin protein ligase 2 [Source:NCB...	[more]

BLAST of SMED30030118 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4


Match Name	E-value	Identity	Description
SMESG000005009.1	9.662e-7	32.53	SMESG000005009.1	[more]
SMESG000051358.1	5.194e-6	30.08	SMESG000051358.1	[more]
SMESG000016986.1	7.775e-6	25.77	SMESG000016986.1	[more]
SMESG000016986.1	7.821e-6	25.77	SMESG000016986.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30030118 ID=SMED30030118|Name=SMED30030118|organism=Schmidtea mediterranea sexual|type=transcript|length=622bp
TATTGATAAGGGTTTTGTCATAATTCCACAAAATTAAAAACAATTTTGTT
AAATCTTTCTTGGCAGCAAGAATTAGTGGAGTTTCATCTTTTAAATTTTT
TATTCCGATTAATTGAGGTTTTTGATTTAATAAAAATTTAGCTTCATCTA
TTTTTTGAGAATAAGTAGCGAAATGAAGCACGGTATCCCCATCAATATCC
ACAGCTAATGGGTCACAACCGCATTTTAGTAAAGTTTTAATTTTTGACAC
AGTTCCATAAAAGGATGCACAATGTAAAGCTGTTTGATTTTTTTGGTCTT
TATCCAATACATTATTATTTCCCACGAACCCTTCTTCAATATCACTAACT
TCTGGATTTTTAATTCTGAGTATTATCTCTTCAAAGGAAGGATTAGGTGT
TGATGGCTTAAATTTTTTCACATCTGTCGTTTCAATTAATAAACTTTCAT
GTATTTTAAAATGCTTCGTTTCATTATCCATATGATATTGAACAATGATA
CATTCTCTATTCCTACTGTAAACCTGGCCAAGTACATTGTGTAATTTTTT
AAAATCAAGTGATGGGAATCTTTTCTCTTTTTGAACTTCCTTTATTTTTT
CATAATCATCGATTAATTTGAC

protein sequence of SMED30030118-orf-1

>SMED30030118-orf-1 ID=SMED30030118-orf-1|Name=SMED30030118-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=207bp
VKLIDDYEKIKEVQKEKRFPSLDFKKLHNVLGQVYSRNRECIIVQYHMDN
ETKHFKIHESLLIETTDVKKFKPSTPNPSFEEIILRIKNPEVSDIEEGFV
GNNNVLDKDQKNQTALHCASFYGTVSKIKTLLKCGCDPLAVDIDGDTVLH
FATYSQKIDEAKFLLNQKPQLIGIKNLKDETPLILAAKKDLTKLFLILWN
YDKTLIN

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR036770	Ankyrin_rpt-contain_sf
IPR002110	Ankyrin_rpt
IPR020683	Ankyrin_rpt-contain_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 144..174 e-value: 46.0 score: 12.9 coord: 111..140 e-value: 1.0 score: 18.4
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 111..143 score: 8.523
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 116..199 e-value: 1.4E-9 score: 38.4
IPR020683	Ankyrin repeat-containing domain	PROSITE	PS50297	ANK_REP_REGION	coord: 111..198 score: 16.335
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	G3DSA:1.25.40.20		coord: 34..203 e-value: 9.4E-19 score: 69.5
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	SSF48403	Ankyrin repeat	coord: 105..207
None	No IPR available	PANTHER	PTHR24124	FAMILY NOT NAMED	coord: 94..190
None	No IPR available	PANTHER	PTHR24124:SF6		coord: 94..190

Main menu

SMED30030118

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

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