Thymus, brain and testes associated

Overview
NameThymus, brain and testes associated
Smed IDSMED30030073
Length (bp)1320
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Thymus, brain and testes associated (SMED30030073) t-SNE clustered cells

Violin plots show distribution of expression levels for Thymus, brain and testes associated (SMED30030073) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Thymus, brain and testes associated (SMED30030073) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Thymus, brain and testes associated (SMED30030073) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030073

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30030073SMESG000053617.1 dd_Smed_v6_4229_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
headSMED30030073SMESG000053617.1 OX_Smed_1.0.21698ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30030073SMESG000053617.1 dd_Smed_v4_4229_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Thymus, brain and testes associated vs. Ensembl Zebrafish
Match: tbata (thymus, brain and testes associated [Source:ZFIN;Acc:ZDB-GENE-121030-4])

HSP 1 Score: 55.8398 bits (133), Expect = 2.845e-8
Identity = 69/271 (25.46%), Postives = 121/271 (44.65%), Query Frame = 2
Query:  263 QHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYS-RSGRILPIIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFV-RDPIPNGENQIIYTPAKNFSSKL 1069
            +H+PHP+RVRH++ L G PVC+V       N  +    P   +  +K + +     S S S+AF +   + D                R+ P   +      E W ++LK++   A    L     + +     +   D  +   K  YS +SGRI+P             R + R  ++ + M EG      ++L+V  LL  ILQT+   +++ WLL A+++EK L+  +++ A+ D   L++    DP+ N E+ ++    +   S L
Sbjct:   48 RHNPHPHRVRHMQGLNGKPVCMV-------NDDWFAFTPLCPHPLIKSQVSV----SGSRSSAFFTPEISSD----------------RSGPRAALIS----ESWREELKDL---ATKITLSAAAKTQHDKREPQKHADDEAPRRKTQYSSQSGRIIPSSSWG---GKKRSRTSHRRTTTHTHMLEG------LELKVLELLCQILQTDSLSMVQQWLLLASDKEKELVQGLLKQAVSDCSSLSLHTSADPL-NTEHLLLEPAGRAHRSSL 274          
BLAST of Thymus, brain and testes associated vs. Ensembl Zebrafish
Match: tbata (thymus, brain and testes associated [Source:ZFIN;Acc:ZDB-GENE-121030-4])

HSP 1 Score: 55.8398 bits (133), Expect = 2.845e-8
Identity = 69/271 (25.46%), Postives = 121/271 (44.65%), Query Frame = 2
Query:  263 QHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYS-RSGRILPIIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFV-RDPIPNGENQIIYTPAKNFSSKL 1069
            +H+PHP+RVRH++ L G PVC+V       N  +    P   +  +K + +     S S S+AF +   + D                R+ P   +      E W ++LK++   A    L     + +     +   D  +   K  YS +SGRI+P             R + R  ++ + M EG      ++L+V  LL  ILQT+   +++ WLL A+++EK L+  +++ A+ D   L++    DP+ N E+ ++    +   S L
Sbjct:   48 RHNPHPHRVRHMQGLNGKPVCMV-------NDDWFAFTPLCPHPLIKSQVSV----SGSRSSAFFTPEISSD----------------RSGPRAALIS----ESWREELKDL---ATKITLSAAAKTQHDKREPQKHADDEAPRRKTQYSSQSGRIIPSSSWG---GKKRSRTSHRRTTTHTHMLEG------LELKVLELLCQILQTDSLSMVQQWLLLASDKEKELVQGLLKQAVSDCSSLSLHTSADPL-NTEHLLLEPAGRAHRSSL 274          
BLAST of Thymus, brain and testes associated vs. Ensembl Xenopus
Match: ENSXETT00000030256.1 (thymus, brain and testes associated [Source:NCBI gene;Acc:100488797])

HSP 1 Score: 65.4698 bits (158), Expect = 4.366e-11
Identity = 72/268 (26.87%), Postives = 121/268 (45.15%), Query Frame = 2
Query:  182 FSMFSIELKSEDSPRYGKSFIKYPGLVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAF--VSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYS-RSGRILPIIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVD 976
            FS  ++   ++ + R+G +F ++    +H+PHP+RV HI+ L GNPVC+V+ E       +S+  P   +  VK         S   +N F    + P  D   + + + G  S+S               E W ++L+ +            C SD      E      S+    +YS  +GRI+P    SR +T    R  +R + +KS  S     D   +L +  LL  ILQT+    I+ WLL+A ++EK L++ +++ A  +
Sbjct:   35 FSSEAMRPPTKSNSRFG-NFSQHSFFSRHNPHPHRVTHIQGLNGNPVCIVNDE-------WSITSPLFPHPMVK---------SQLLTNIFGVTCSMPIGDPHGNKHPYLGAGSVS---------------EAWREELRELAAKV--------CTSDKPPVKKEVEEPRRST----LYSAETGRIIPPS--SRAMTRQGSRKANRNSINKSKNSATLFQDQ--ELLILELLSQILQTDSLTAIQQWLLSAGQKEKDLVMGMIQTATAN 254          
BLAST of Thymus, brain and testes associated vs. TrEMBL
Match: A0A1S3I2F2 (uncharacterized protein LOC106160400 OS=Lingula unguis OX=7574 GN=LOC106160400 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.980e-27
Identity = 78/246 (31.71%), Postives = 130/246 (52.85%), Query Frame = 2
Query:  257 LVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHF-PPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYSRSGRILPIIGISRPLTSPEKRPNSRLNSSKSFMS--EGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEE 985
              +H+PHP RVRH+K L   P+C V+ +    + +YSL +  PN+Y+D K     L +    ++    S    ++TI  L +FTGL S  FR +    +  V   E W  +LK +T+ A     +     D +L   E   + ++ P  +  S +GR++P    SR +T    R  SR N  + F S  E  + + +++  VF +L  ILQT++   ++ WL+++ EREKAL++D++R A+  +EE
Sbjct:   53 FTRHNPHPRRVRHLKGLLDMPICSVNDDGFFASPRYSLFYNSPNSYNDAKSMQRGLGVTLPVNAINVNSTLHPINTITGLQHFTGLNSYPFREKAVPKVGMVPQTEAWRDELKMLTELA-----QYNPSEDRRLKPEEKKVEEMTRPKTQYSSETGRLIP--PPSRAMT----RQGSRQNKRQQFFSQYEHIAPEPDMETMVFQMLAQILQTDDPNAVQAWLVSSGEREKALVLDMIRAAITSREE 287          
BLAST of Thymus, brain and testes associated vs. TrEMBL
Match: A0A1I8H186 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.347e-25
Identity = 96/316 (30.38%), Postives = 162/316 (51.27%), Query Frame = 2
Query:  224 RYGKSFIKYPGLVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPF--LKNKCLSDNQLTMIETGRDSISSPMKR-----------MYSR-SGRILPIIGISRPLTSPEKRPNSRLNSSKSFMS-EGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDPIPNGENQIIYTPAK-NFSSKLPVISENSNSEK-IDQLVI 1120
            R+G+   K     +H+PHP+R  HIK L G P+ VV  +  +++ +  L FPPN ++D KL + +L L  N+      S +  LDT+N L YFT L++ SF+ +    +  V   E W +QL+++TD    P   +     +D +  M      +   P ++           MYSR +GR++P    SR ++    R + R   +    + E  S   +++  VF +L  ILQT +   ++ WL++A++REKAL++D++R A+ +K     +  D  P GE   ++ P        LP I+E+ +  K +D+LVI
Sbjct:   29 RFGQ-LSKSSFFARHNPHPHRCVHIKGLLGLPIPVVVDDCDDSSYETHLCFPPNGHNDEKLASQRLLLPVNAIH--LNSHKHPLDTVNGLQYFTNLRNYSFKEKAVPRLGLVPVTESWREQLRSLTDQTFHPEQPVAPAPAADGRGDMNLRREATYPDPARQQQPEQSQTARPMYSRQTGRLIP--PPSRAMSRRPLRSSRRSTPAHVLSNYEHISAAPDLESAVFSMLCQILQTEDFNAVQAWLVSASDREKALVMDVIRSAVANK--AAYYEAD--PTGE---VFQPTNIREDLHLPPIAEDPDRPKRVDRLVI 332          
BLAST of Thymus, brain and testes associated vs. TrEMBL
Match: A0A1I8JKL9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.491e-25
Identity = 93/305 (30.49%), Postives = 157/305 (51.48%), Query Frame = 2
Query:  257 LVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPF--LKNKCLSDNQLTMIETGRDSISSPMKR-----------MYSR-SGRILPIIGISRPLTSPEKRPNSRLNSSKSFMS-EGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDPIPNGENQIIYTPAK-NFSSKLPVISENSNSEK-IDQLVI 1120
              +H+PHP+R  HIK L G P+ VV  +  +++ +  L FPPN ++D KL + +L L  N+      S +  LDT+N L YFT L++ SF+ +    +  V   E W +QL+++TD    P   +     +D +  M      +   P ++           MYSR +GR++P    SR ++    R + R   +    + E  S   +++  VF +L  ILQT +   ++ WL++A++REKAL++D++R A+ +K     +  D  P GE   ++ P        LP I+E+ +  K +D+LVI
Sbjct:   39 FARHNPHPHRCVHIKGLLGLPIPVVVDDCDDSSYETHLCFPPNGHNDEKLASQRLLLPVNAIH--LNSHKHPLDTVNGLQYFTNLRNYSFKEKAVPRLGLVPVTESWREQLRSLTDQTFHPEQPVAPAPAADGRGDMNLRREATYPDPARQQQPEQSQTARPMYSRQTGRLIP--PPSRAMSRRPLRSSRRSTPAHVLSNYEHISAAPDLESAVFSMLCQILQTEDFNAVQAWLVSASDREKALVMDVIRSAVANK--AAYYEAD--PTGE---VFQPTNIREDLHLPPIAEDPDRPKRVDRLVI 332          
BLAST of Thymus, brain and testes associated vs. TrEMBL
Match: A0A267H1S7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006934g1 PE=4 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.208e-24
Identity = 93/305 (30.49%), Postives = 157/305 (51.48%), Query Frame = 2
Query:  257 LVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPF--LKNKCLSDNQLTMIETGRDSISSPMKR-----------MYSR-SGRILPIIGISRPLTSPEKRPNSRLNSSKSFMS-EGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDPIPNGENQIIYTPAK-NFSSKLPVISENSNSEK-IDQLVI 1120
              +H+PHP+R  HIK L G P+ VV  +  +++ +  L FPPN ++D KL + +L L  N+      S +  LDT+N L YFT L++ SF+ +    +  V   E W +QL+++TD    P   +     +D +  M      +   P ++           MYSR +GR++P    SR ++    R + R   +    + E  S   +++  VF +L  ILQT +   ++ WL++A++REKAL++D++R A+ +K     +  D  P GE   ++ P        LP I+E+ +  K +D+LVI
Sbjct:   39 FARHNPHPHRCVHIKGLLGLPIPVVVDDCDDSSYETHLCFPPNGHNDEKLASQRLLLPVNAIH--LNSHKHPLDTVNGLQYFTNLRNYSFKEKAVPRLGLVPVTESWREQLRSLTDQTFHPEQPVAPAPAADGRGDMNLRREATYPDPARQQQPEQSQTARPMYSRQTGRLIP--PPSRAMSRRPLRSSRRSTPAHVLSNYEHISVAPDLESAVFSMLCQILQTEDFNAVQAWLVSASDREKALVMDVIRSAVANK--AAYYEAD--PTGE---VFQPTNIREDLHLPPIAEDPDRPKRVDRLVI 332          
BLAST of Thymus, brain and testes associated vs. TrEMBL
Match: K1R2A0 (Protein SPATIAL OS=Crassostrea gigas OX=29159 GN=CGI_10026923 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 7.757e-22
Identity = 86/300 (28.67%), Postives = 144/300 (48.00%), Query Frame = 2
Query:  257 LVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYSR-SGRILPIIGISRPLTSPEKRPNSRLNSSKSFMS-EGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDPIPN--GENQIIYTPAKNFSSKLPVISEN----SNSEKIDQLVIHSDP 1132
              +H+PHP+RVRHIK L   P+C V+ +    + KYSL FPPN +++ ++   K  +  N+  N      P ++T+  L YFTGL S  FR +    +  V   E W  +L+ +T        K      ++L   +     +    K  YS  +GRI+P    SR ++    +  SR    + F + +  + + +++  V  +L  ILQT +   ++ WL +A EREK +++D++R A+  + +   + R+  P    E Q+         +KLP I+E        EK D+L    DP
Sbjct:   59 FTRHNPHPSRVRHIKGLLDVPICAVNDDGYFASPKYSLQFPPNAFNNKQMNKWKGQVPVNA-INVNSRLHP-INTVTGLQYFTGLNSYPFREKAVPRVGMVPVTEQWRDELQQLT--------KAIGFGTDELAPQKPQAPGVPERPKTQYSETTGRIIPP--PSRAMS--RGKTGSRQGRDRYFGTLQHIAPEPDMETMVLQMLCQILQTEDINAVQAWLCSAGEREKTVVMDLIRSAVGSQSDY--YQREVRPEYVEEGQV---------TKLPPINETGQIVGGGEKADRLTFK-DP 332          
BLAST of Thymus, brain and testes associated vs. Planmine SMEST
Match: SMESG000053617.1 (SMESG000053617.1)

HSP 1 Score: 691.804 bits (1784), Expect = 0.000e+0
Identity = 357/360 (99.17%), Postives = 358/360 (99.44%), Query Frame = 2
Query:  188 MFSIELKSEDSPRYGKSFIKYPGLVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYSRSGRILPIIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDPIPNGENQIIYTPAKNFSSKLPVISENSNSEKIDQLVIHSDPQPKFEVXXXXXXXXXXXXXXXXISTARPQTRGQSSWMQSESIVVN 1267
            MFSIELKSEDSPRYGKSFIKYPGLVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLS+NQLTMIETGRDSISSPMKRMYS SGRILPIIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRD IPNGENQIIYTPAKNFSSKLPVISENSNSEKIDQLVIHSDPQPKFEVPPTRPPTSSTFRPNTRISTARPQTRGQSSWMQSESIVVN
Sbjct:    1 MFSIELKSEDSPRYGKSFIKYPGLVQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSENQLTMIETGRDSISSPMKRMYSSSGRILPIIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDSIPNGENQIIYTPAKNFSSKLPVISENSNSEKIDQLVIHSDPQPKFEVPPTRPPTSSTFRPNTRISTARPQTRGQSSWMQSESIVVN 360          
BLAST of Thymus, brain and testes associated vs. Planmine SMEST
Match: SMESG000022377.1 (SMESG000022377.1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.434e-14
Identity = 77/266 (28.95%), Postives = 120/266 (45.11%), Query Frame = 2
Query:  221 PRYGKSFIKYPGLVQHSPHPNRVRHIKTLFGNPVC-VVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNF-----NMSRVKSLEGWEKQLKNITDSALDPFLKNKCLSDNQL-TMIETGRDSISSPMKRMYSRSGRILP------IIGISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNENEVIKTWLLTATEREKALMVDIVRCALVDK 979
            P+     I++    +H+PHP RV H   L   P+C VVD              PP              L S    +  V   P L   N+LN   GL    FRN P        +  V   E W ++L+ +T+S + P  +   ++  QL T+IE     I  P+  +  R+   +P      I+   +  TS  K P  +  SS  F+ +  S + + + QVF LLG ILQT++ + +KTWL  A++REKA ++ +++ A+  K
Sbjct:   24 PKKQTRTIRWNLTARHNPHPRRVNHFYGLQNMPICHVVD--------------PPL-------------LTSIKCVDPSVDIDPVLVPSNTLN---GLPIFRFRNIPIKEKAIPQLGIVPIQENWRRELRALTESIIVPISEEIGITQGQLQTLIEYHPYPICRPITSIIQRT--FVPKMNQDFIMEREKLKTSTPKFPPIKPLSSDKFLWDE-SQEGDYESQVFALLGQILQTSDVDYVKTWLTVASDREKATVMGMLQSAVESK 256          
BLAST of Thymus, brain and testes associated vs. Planmine SMEST
Match: SMESG000081805.1 (SMESG000081805.1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.251e-11
Identity = 35/114 (30.70%), Postives = 55/114 (48.25%), Query Frame = 2
Query:  260 VQHSPHPNRVRHIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSDSNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLKNIT 601
            ++H+PHP  VRH+K   G P  +V++E+ N      +       +       K  + +  DS +FV     LD I SL YFTGLQ+ SF+      +   +    W K L+N++
Sbjct:   31 LRHNPHPLYVRHVKGFLGQPTQIVETEEDN------VILSQGTVEGFIQDCVKRGVWNYIDSKSFVRKTRDLDEILSLQYFTGLQNFSFKENYLPYLGYREYFSSWRKNLRNLS 138          
The following BLAST results are available for this feature:
BLAST of Thymus, brain and testes associated vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tbata2.845e-825.46thymus, brain and testes associated [Source:ZFIN;A... [more]
tbata2.845e-825.46thymus, brain and testes associated [Source:ZFIN;A... [more]
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BLAST of Thymus, brain and testes associated vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000030256.14.366e-1126.87thymus, brain and testes associated [Source:NCBI g... [more]
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BLAST of Thymus, brain and testes associated vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3I2F21.980e-2731.71uncharacterized protein LOC106160400 OS=Lingula un... [more]
A0A1I8H1867.347e-2530.38Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8JKL97.491e-2530.49Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267H1S71.208e-2430.49Uncharacterized protein OS=Macrostomum lignano OX=... [more]
K1R2A07.757e-2228.67Protein SPATIAL OS=Crassostrea gigas OX=29159 GN=C... [more]
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BLAST of Thymus, brain and testes associated vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Thymus, brain and testes associated vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000053617.10.000e+099.17SMESG000053617.1[more]
SMESG000022377.11.434e-1428.95SMESG000022377.1[more]
SMESG000081805.13.251e-1130.70SMESG000081805.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030073 ID=SMED30030073|Name=Thymus, brain and testes associated|organism=Schmidtea mediterranea sexual|type=transcript|length=1320bp
AATTAGATGAACTATTTGATGGTGGTAACAATAGACGAAGCCAGCAACCA
TTGGTATGGTGGTGCAGGCTCCATTGTCATTTCAATTAACTAAATGCAAC
AGAGGTAGATAAAATCTACACGGATTGCTAGGTAACTGTCAGATGAACTT
TCTAACATTGAAAAAGGAAAGAAAATCAGATTTTTCAATGTTTTCTATTG
AATTAAAATCTGAGGACTCACCAAGATATGGAAAGTCTTTTATAAAGTAT
CCCGGATTAGTGCAACACAGTCCTCATCCAAACCGAGTTAGACATATCAA
AACTCTATTCGGAAATCCGGTGTGTGTTGTCGACTCAGAAGACGGAAACA
CCAACCGGAAATATAGTCTACATTTTCCGCCCAATAATTATGATGACGTT
AAATTGAAAACTACAAAACTCAATTTGCACTCGAATTCCGATTCAAATGC
TTTCGTTTCTGCTCGGCCGACTTTAGACACCATCAATTCGTTGAATTATT
TCACGGGACTTCAAAGCATGTCGTTCCGCAATCGGCCTAATTTCAATATG
AGTCGCGTGAAAAGTTTAGAAGGGTGGGAAAAACAACTGAAAAACATTAC
CGATTCTGCTCTAGATCCGTTTTTAAAAAACAAATGTCTTTCGGATAACC
AATTAACGATGATAGAAACGGGAAGAGATTCAATTTCTAGTCCGATGAAA
AGAATGTATTCGAGATCAGGTCGAATATTGCCAATTATTGGAATTTCCCG
GCCATTAACATCTCCTGAAAAAAGACCAAATTCAAGATTAAATTCATCCA
AATCTTTCATGTCGGAAGGCGGTTCTAATGACATTAACATTGACTTACAA
GTGTTTCATTTGTTAGGAGGAATCCTTCAGACTAACGAAAATGAAGTCAT
CAAAACGTGGCTATTAACAGCGACAGAAAGAGAAAAAGCCTTGATGGTTG
ATATTGTCAGATGTGCATTGGTGGATAAAGAAGAGTTGAACATTTTTGTC
AGAGATCCGATTCCGAATGGGGAAAATCAAATTATTTACACTCCCGCAAA
AAATTTCAGCTCAAAACTTCCGGTCATTTCAGAAAATTCCAATTCGGAAA
AAATAGATCAGCTTGTTATTCATTCAGATCCACAGCCGAAATTTGAAGTC
CCTCCAACTAGACCGCCGACTTCATCAACATTCAGACCGAACACGAGAAT
ATCGACAGCAAGACCTCAGACGAGGGGTCAGTCATCCTGGATGCAGAGCG
AATCAATTGTAGTCAATTAGAACTTCACAGATATACTGAGATTCAAATAT
TTTCAATTACAACAATTTTA
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protein sequence of SMED30030073-orf-1

>SMED30030073-orf-1 ID=SMED30030073-orf-1|Name=SMED30030073-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=376bp
MNFLTLKKERKSDFSMFSIELKSEDSPRYGKSFIKYPGLVQHSPHPNRVR
HIKTLFGNPVCVVDSEDGNTNRKYSLHFPPNNYDDVKLKTTKLNLHSNSD
SNAFVSARPTLDTINSLNYFTGLQSMSFRNRPNFNMSRVKSLEGWEKQLK
NITDSALDPFLKNKCLSDNQLTMIETGRDSISSPMKRMYSRSGRILPIIG
ISRPLTSPEKRPNSRLNSSKSFMSEGGSNDINIDLQVFHLLGGILQTNEN
EVIKTWLLTATEREKALMVDIVRCALVDKEELNIFVRDPIPNGENQIIYT
PAKNFSSKLPVISENSNSEKIDQLVIHSDPQPKFEVPPTRPPTSSTFRPN
TRISTARPQTRGQSSWMQSESIVVN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0048484enteric nervous system development
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR037394TBATA-like
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037394Protein TBATA-likePFAMPF15256SPATIALcoord: 39..276
e-value: 6.4E-22
score: 78.9
IPR037394Protein TBATA-likePANTHERPTHR33772FAMILY NOT NAMEDcoord: 40..319
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 345..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..375
NoneNo IPR availablePANTHERPTHR33772:SF1THYMUS, BRAIN AND TESTES-ASSOCIATEDcoord: 40..319