Rho_N domain-containing protein

Overview
NameRho_N domain-containing protein
Smed IDSMED30030071
Length (bp)2908
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rho_N domain-containing protein (SMED30030071) t-SNE clustered cells

Violin plots show distribution of expression levels for Rho_N domain-containing protein (SMED30030071) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rho_N domain-containing protein (SMED30030071) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rho_N domain-containing protein (SMED30030071) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030071

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30030071 Contig22037uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30030071 Contig22037newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neuronSMED30030071 Contig12427newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
neuronSMED30030071 Contig12427uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30030071 Contig12427newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30030071 Contig12427uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Match: ZC53.4 (pep chromosome:WBcel235:X:1925750:1937689:1 gene:WBGene00022511.1 transcript:ZC53.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC53.4)

HSP 1 Score: 185.267 bits (469), Expect = 1.761e-47
Identity = 102/291 (35.05%), Postives = 170/291 (58.42%), Query Frame = 3
Query: 1230 LADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGL-------TFVNKHYIAASEDTEL----LYIDINNLYGWALSQSLPYADFTW-------VYDGLDEVLSECANVVD-IEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSII 2045
            +AD F+ FRA ++    L+P  ++  PG++W + L   +  ++ L D+EM+   E GIRGG+       + VN  Y     + +L    LY+D NNLYGWA+ Q LP  DF +       + +G  EV S+  + ++ ++ L  G   EVD+E P  +H   ND P+ PE +   N  ++KL  T + ++ +V+H+  L   ++LG +++ IHR I FK++     YI+ NT+ R N K +  +D YKL NNS++GK++EN+++R+++RL   ++K+   ASKP F++ II
Sbjct: 1117 IADVFENFRALSLRVYQLDPCWYFTSPGLAWDAMLKKTKVELDKLHDVEMYLMIEKGIRGGIVNAVKRYSKVNNKYTKNFNEKQLTNFLLYLDANNLYGWAMCQMLPTGDFKFETQSLISILNGEAEVKSDLKDYINYLKKLDRGCIFEVDLEYPKEIHSRHNDSPLCPENVKIDNHVIKKLCNTLFDREKYVIHYENLLQCLQLGLKLKKIHRIITFKESNWLASYIEMNTNPRKNAKNDFEKDFYKLMNNSVFGKTMENVRERVDVRLVTDEDKIKKLASKPNFKRRII 1407          

HSP 2 Score: 80.1073 bits (196), Expect = 5.733e-15
Identity = 63/196 (32.14%), Postives = 96/196 (48.98%), Query Frame = 2
Query:  377 VTPPFVIYADFESIL---------PLDIRHHQIHMPIAAGLLLINNYTNTKQYYN---FIGDDCVLAFLKKIDEIALSIV-LPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHIL------KYGLNNFSSWELNIIPTTTEKFISL 907
            V   ++IYADFESI+         P +     +   +A+   ++   + TK+ +N   +IG +  L F K I +I+  I+ LP  ++          F + +E   C+  Y  +     KV+DH H++G+Y G  C  CN+ RR    +PV FHNL+GYD H I+      KY   N    E+  I + TEKFIS 
Sbjct:  872 VKHRYIIYADFESIIWKFSNAHYKPNESWTMDVGEHVASRFCVVVVDSFTKKIHNMKSYIGYNATLEFNKYIIKISQEIMSLPSVRMKKLTQDEWTEFNKNQECPHCQVQYNTEDMKTTKVRDHDHWTGEYRGPFCGSCNILRRKNNFIPVFFHNLKGYDSHLIVGCLESTKYL--NEHEIEIEKISSNTEKFISF 1065          
BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Match: H12I13.3 (pep chromosome:WBcel235:II:6071386:6073251:-1 gene:WBGene00019192.1 transcript:H12I13.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:H12I13.3)

HSP 1 Score: 106.686 bits (265), Expect = 1.131e-23
Identity = 73/253 (28.85%), Postives = 120/253 (47.43%), Query Frame = 3
Query: 1788 GAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYSRKLDSVIGNFKDE*GSWVCGMGFLKTQVL*FVKY*RYDEG*WGMKLLGQRLSTSHI*IVLIMT*YSRCL 2546
            G +++ IHR + FK++   K+YI  NT+LR   K +  +D +KL NNS++GK++EN++ R+N+RL   D+  +    KP F+ S II ++L++  L K  +  D+P Y    V+                  +  +  +I +   DTDS   EV+   +   +  M ++    S+Y   HPLY      VIG FKDE        G        F+K  +YD    G +    +    ++   L +  + RCL
Sbjct:  299 GLKLKKIHRILTFKESAWMKKYIMLNTNLRKQAKNDFEKDFFKLMNNSVFGKTMENIRNRVNIRLVTDDDSALKLIRKPNFKNSTIITEDLLSVELTKTKLVFDKPIYFHYDVM------------------QKHYGDKIKLCYQDTDSLIYEVETEDIYEDMGNM-KEWFDFSDYPKNHPLYDESNKKVIGKFKDELNGKNMDEGV-------FLKPKQYDFNVEGAEKKKSKGVKKNVTKTLTLDDFKRCL 525          
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A2L2YHT1 (Uncharacterized protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.406e-105
Identity = 172/469 (36.67%), Postives = 259/469 (55.22%), Query Frame = 3
Query: 1044 KAIFPYDFAKSLEVLESTTELPPIWG--------SVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLT-------FVNKHYIAASEDTE-----LLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDL--LYTSNYDPAHPLYS--RKLDSVIGNFKDE 2378
            K ++PYD+       E TT LPP           S+S ++Y  A+++W      ++ +Y   Y+K DV LLAD F+ FR   + +  L+P  ++  PG+SW + L      IELL D +M  F E GIRGG++         N  Y+ AS D+      LLY+D NNLYGWA+SQ LP  DF WV    D VLS   +       P G+  EVD++ P NLHD  +D P+ PE   PP  K ++L+ T Y K+ +VLH+R LK Y++ G  +  +HR ++F Q+P  K YI  NT LR +   E  +  +KL NN+++GK++EN++ R+N++LC+S++K     S+  FR   I+ + L+A H+ +  I  ++P  IG  VLD+SK+ MY+  Y    K + ++  +I ++  DTDSF  ++     + +   M  DL    TS+Y P   +Y   RK   V+G  KDE
Sbjct:  552 KGVYPYDYIDHESKFEETT-LPPQRAFYSKLNDCSISNEDYKHAEEVWQIFKMSSLGEYSDLYVKTDVLLLADVFENFRDVCMRHYNLDPAWYFTAPGLSWDAMLKKTNISIELLTDYDMILFIESGIRGGISQCSHRYGSANNKYMEASYDSSKPSNYLLYLDANNLYGWAMSQPLPLQDFKWVEPPTD-VLSISDDG------PLGFIFEVDLDYPKNLHDNHSDLPLAPETKIPPGCKDKRLLTTLYHKEKYVLHYRNLKFYLQQGLILRKVHRVLQFSQSPWLKSYIDLNTSLRKDATNEFEKSFFKLMNNAVFGKTMENIRNRVNVKLCSSEKKAEKLISRCNFRDRTILTEQLVAIHMGRTEITFNKPISIGMAVLDISKILMYKFHY---TKMKTKYEEKIKLLYTDTDSFIYDI---TTEDVYSDMNEDLDSFDTSDY-PEDNIYGTPRKNKKVLGVMKDE 1005          

HSP 2 Score: 125.176 bits (313), Expect = 1.406e-105
Identity = 83/226 (36.73%), Postives = 127/226 (56.19%), Query Frame = 2
Query:  368 VKTVTP-PFVIYADFESIL-PLD----------IRHHQIHMPIAAGLLLINNYTNTKQYYNFIGDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLC-KKFIRD--KVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYIN-KLPVRFIDSMQFVNSSLAKAVKTLT 997
            VK   P PFVIYADFES+L P+D             +Q H PI+    +      +K  Y + G D    F++K+ E AL I   + K +   P+  L+ K+ ++++    CY+C  +F  +  KVKDHCH + ++ GA+C  CN+  ++   +PV+FHNL GYD H  +K    N  +  +N+IP  TEK+IS   Y++ K+ +RFIDS +F+ SSL +  + LT
Sbjct:  310 VKHTQPVPFVIYADFESLLKPIDSCGPSSGASFTEKYQKHEPISFCYYVCGIAGLSKPPYVYRGKDAAKIFMEKMREEALII---HEKYNNPLPMKSLTEKQTQQYESATNCYVCDSEFSVENYKVKDHCHVTQEFRGASCNNCNMQTKVPNFIPVIFHNLSGYDSHLFIKELGKNEEN--INVIPENTEKYISFSEYVSGKIFLRFIDSYRFMASSLDQLARNLT 530          
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: K7JB67 (Uncharacterized protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 2.943e-105
Identity = 170/474 (35.86%), Postives = 262/474 (55.27%), Query Frame = 3
Query: 1035 FRAKAIFPYDFAKSLEVLESTTELPPIW--------GSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLT------------FVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDI-EILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMI---VYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYS--RKLDSVIGNFKDE 2378
             + K +FPYD+  SL  L     LP             +S ++Y  A+++W + N +T+  Y   YLK DV LLA+ F+ FR+  +    L+P ++Y  PG+SW + L   Q  ++LL D++M  F E GIRGG++            ++N+ Y A +++  L+Y D NNLYGWA++++LP   F WV D       +  N  ++ +  P GY +EVD+E P  LH    D P   E MCPP SK +KLM T Y KKN+V+H+R LK  +  G  ++ IHRA++F+Q+   K YI  N+D+R N K E  + L+KLFNN++YGK++EN +K + ++L    E         +KPTF    I D+ L+A  + +  +C+ +P YIG +VLDLSK  +Y   Y  + + R    C+  ++  DTDS   EV +  +  ++R  I +   TS+Y P +  +   R    ++G  KDE
Sbjct:  593 LKRKGVFPYDYVSSLAKLNEVN-LPTKEDFFSSLYDAHISDEDYEHAKRVWQELNVQTLGQYSDLYLKTDVILLAEVFENFRSNCLQAYGLDPAHYYTTPGLSWDAMLKYTQVRLDLLTDIDMLMFVERGIRGGISQCCNRYAKANNPYMNEGYDANADEKYLVYYDANNLYGWAMTEALPSGKFKWVAD------LQTPNFFNVPDDGPKGYILEVDLEYPIMLHGAHRDMPFCAEHMCPPGSKQKKLMTTLYDKKNYVIHYRALKQALAHGLRLKKIHRALEFEQSKWLKPYIDLNSDMRKNAKNEFEKMLFKLFNNAVYGKTMENERKHVEVKLVTKWEGRYGAEALIAKPTFHNCTIFDEYLVAVQMFRAEVCIKKPIYIGLSVLDLSKTLIYRFHYDYMLE-RAGDKCK--LLYTDTDSLVYEVSNLNVYEMMRIDIHE-FDTSDY-PVNNQFDMPRVNKKIVGLMKDE 1054          

HSP 2 Score: 100.523 bits (249), Expect = 2.943e-105
Identity = 68/206 (33.01%), Postives = 105/206 (50.97%), Query Frame = 2
Query:  362 NYVKTVTPPFVIYADFESIL-PLDI--RHHQIHMPIAAGLLLINNYTNT----KQYYNFIGDDC-VLAFLKKIDEIALSIVLPYYKVHCQKPIG--PLSFKEKREFKDCRTCYLCKKFIRDK---VKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRF 940
            N+V     PFV+YADFE +L P+D   R +Q H P + G  +  ++ +T    K Y   I +D     F+  +  +AL +   Y     + PI    L+ ++K EF   R C++C K   +K   V+DHCH +G+Y GAA   CN++ +  + +PVVFHNL GYD H  +K     F       +P++ +K  S +  +  L   F
Sbjct:  380 NFVNRNRVPFVVYADFECLLEPVDDHKRAYQQHKPYSVGYFVKCSFNDTLSVYKSYRQSIEEDTPARWFVNSLHALALELQNMY-----KNPIAMRDLTDQQKLEFSMARKCHICNKPFNEKDAKVRDHCHLTGEYRGAAHNSCNLNYQDSRTIPVVFHNLSGYDAHLFIKEIATCFPGHSFRFMPSSLDKLSSYLTELGMLERVF 580          

HSP 3 Score: 51.9878 bits (123), Expect = 2.943e-105
Identity = 33/95 (34.74%), Postives = 46/95 (48.42%), Query Frame = 2
Query: 2468 NEVVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWV-GRNSSLAFGHYLGVDVIDDE*VTHSPRYESTHSPSYD 2749
            + VV   IE   Y+ C   + I T  Q  I  R H+LFT K  KIAL+ + DKR    G+  +L +GHY    +++DE  T +        P  D
Sbjct: 1089 SSVVKATIEFDDYMTCLQENAILTREQRNIRTRQHKLFTEKEIKIALSARNDKRFLQEGKTDTLPWGHY---SIVEDEQPTAALPVRGLEQPIAD 1180          

HSP 4 Score: 21.9422 bits (45), Expect = 2.943e-105
Identity = 10/29 (34.48%), Postives = 14/29 (48.28%), Query Frame = 3
Query:  231 TSCKIQI*VHWCPHCLYGFRLKPKFDKHV 317
            TS K    +H C  CL+ F+ + K   H 
Sbjct:  327 TSRKHSNKIHLCDRCLHYFQTEEKLASHT 355          
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A224XH84 (Putative dna polymerase type b (Fragment) OS=Panstrongylus lignarius OX=156445 PE=4 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 6.819e-105
Identity = 181/472 (38.35%), Postives = 264/472 (55.93%), Query Frame = 3
Query: 1044 KAIFPYDFAKSLEVLESTTELPP--------IWGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAAS-----------EDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYK-ELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYSRKLDSVIGNFKDE*GSWVCG 2399
            K +FPYDF  S + LE  T LP            S+S ++Y  AQ++W+  NC+T+ DY   YL+LD  LLAD F+ FR+  +  + L+P ++Y +PG+SW + L      +ELLQD E   F E GIRGGL  V+  Y  A+           E T + Y D NNLYG+++++ LPY +FTW+    +E+  E A+    +   YGY +EVDI+ P  LH   N  P  PE +    +K  KL+     K N+++H+  LK  ++ G ++E IHR +KF+Q+   K YI  N  LR+       +DLYKL+NNS+YGK++EN++ R N++L   ++++    +KP+F    +  +NL+A HL K  I  DRP +IG ++LDLSK  MYE  Y   L KY  +    + +   DTDSF   +K   L   +  MI + L TS+Y   HP +S K   V+G FKDE     CG
Sbjct:  490 KGVFPYDFVTSWDRLE-VTSLPSREDFYNRLTGASISDEDYLHAQKVWSAFNCQTLGDYSDHYLQLDTLLLADCFEHFRSITLNTHQLDPCHYYTLPGLSWDAMLKFTGVRLELLQDYEQIMFIESGIRGGLCQVSHRYCKANNQYLADYNPNLETTYISYQDCNNLYGFSMTKCLPYGEFTWI--SSEELNLETAD----DNGEYGYILEVDIDYPKKLHRLHNSLPFLPETLVV--NKQRKLIPNLNDKHNYIVHYVTLKQALKHGLQIEKIHRILKFRQSNWLKSYIDYNATLRAVATNTFDKDLYKLYNNSVYGKTMENIRNRKNIKLVCDNKQLEKLIAKPSFLDRTLYSENLVAVHLAKTKIKFDRPIFIGMSILDLSKNVMYEYYYDIMLPKYGLD---NLILTYMDTDSFIYYIKTNDLYEDILGMI-EYLDTSDYPVNHPCHSLKNKKVLGKFKDE----ACG 944          

HSP 2 Score: 114.775 bits (286), Expect = 6.819e-105
Identity = 82/225 (36.44%), Postives = 118/225 (52.44%), Query Frame = 2
Query:  389 FVIYADFESIL-----------PLDIRHHQ-----IHMPIAAGLLLINNYTNTKQYYNFIGDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKK--FIRDK-VKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINK-LPVRFIDSMQFVNSSLAKAVKTLTDL 1003
            FV+YADFESIL            +  + H+      ++     +  +++   TK Y  + G +    F+K I  IA  +   Y     + P+ PLS  EK  F    TCY+C K  F  DK V DHCH +GK+ GAA  KCN+  ++   LPV+ HNL GYD H I+K     + +  +++IP T EK+IS    INK + VRF+D+ +F+ +SL   VK L  L
Sbjct:  254 FVVYADFESILHPIKKGLVDKFSIPFQKHEAMSFCTYVKFTDDMEEMSSNLPTKPYI-YRGKNAPAHFMKFIKNIAEEVSKIY---KLKTPMIPLSENEKIAFDSATTCYMCNKTFFKNDKKVHDHCHITGKFRGAAHNKCNLRYQVPSVLPVLIHNLSGYDSHFIIKEL--GYDNKRIDVIPHTEEKYISFSKQINKDISVRFLDTFRFMPTSLNNLVKNLPQL 472          

HSP 3 Score: 27.7202 bits (60), Expect = 6.819e-105
Identity = 16/54 (29.63%), Postives = 27/54 (50.00%), Query Frame = 2
Query: 2489 IEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWVGRNS 2650
            I  + Y+ C N +I        I  + HQLFT + +K++L+   D +  +G N 
Sbjct:  982 IRFKDYVKCLNGNINMRTVVNCIQSKQHQLFTVEINKLSLS-PFDTKRVIGSNG 1034          
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A2L2YHS0 (Uncharacterized protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 7.599e-105
Identity = 170/468 (36.32%), Postives = 259/468 (55.34%), Query Frame = 3
Query: 1044 KAIFPYDFAKSLEVLESTTELPPIWG--------SVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLT-------FVNKHYIAASEDTE----LLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDL--LYTSNYDPAHPLYS--RKLDSVIGNFKDE 2378
            K ++PYD+       E TT LPP           S+S ++Y  A+++W      ++ +Y   Y+K DV LLAD F+ FR   + +  L+P  ++  PG+SW + L      IELL D +M  F E GIRGG++         N  Y+ A + ++    LLY+D NNLYGWA+SQ LP  DF WV    D VLS   +       P G+  EVD++ P NLHD  +D P+ PE   PP  K ++L+ T Y K+ +VLH+R LK Y++ G  +  +HR ++F Q+P  K YI  NT LR +   E  +  +KL NN+++GK++EN++ R+N++LC+S++K     S+  FR   I+ + L+A H+ +  I  ++P  IG  VLD+SK+ MY+  Y    K + ++  +I ++  DTDSF  ++     + +   M  DL    TS+Y P   +Y   RK   V+G  KDE
Sbjct:  552 KGVYPYDYIDHESKFEETT-LPPQRAFYSKLNDCSISNEDYKHAEEVWQIFKMSSLGEYSDLYVKTDVLLLADVFENFRDVCMRHYNLDPAWYFTAPGLSWDAMLKKTNISIELLTDYDMILFIESGIRGGISQCSHRYGSANNKYMEAYDSSKSSNYLLYLDANNLYGWAMSQPLPLQDFKWVEPPTD-VLSISDDG------PLGFIFEVDLDYPKNLHDNHSDLPLAPETKIPPGCKDKRLLTTLYHKEKYVLHYRNLKFYLQQGLILRKVHRVLQFSQSPWLKSYIDLNTSLRKDATNEFEKSFFKLMNNAVFGKTMENIRNRVNVKLCSSEKKAEKLISRCNFRDRTILTEQLVAIHMGRTEITFNKPISIGMAVLDISKILMYKFHY---TKMKTKYEEKIKLLYTDTDSFIYDI---TTEDVYSDMNEDLDSFDTSDY-PEDNIYGTPRKNKKVLGVMKDE 1004          

HSP 2 Score: 125.176 bits (313), Expect = 7.599e-105
Identity = 83/226 (36.73%), Postives = 127/226 (56.19%), Query Frame = 2
Query:  368 VKTVTP-PFVIYADFESIL-PLD----------IRHHQIHMPIAAGLLLINNYTNTKQYYNFIGDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLC-KKFIRD--KVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYIN-KLPVRFIDSMQFVNSSLAKAVKTLT 997
            VK   P PFVIYADFES+L P+D             +Q H PI+    +      +K  Y + G D    F++K+ E AL I   + K +   P+  L+ K+ ++++    CY+C  +F  +  KVKDHCH + ++ GA+C  CN+  ++   +PV+FHNL GYD H  +K    N  +  +N+IP  TEK+IS   Y++ K+ +RFIDS +F+ SSL +  + LT
Sbjct:  310 VKHTQPVPFVIYADFESLLKPIDSCGPSSGASFTEKYQKHEPISFCYYVCGIAGLSKPPYVYRGKDAAKIFMEKMREEALII---HEKYNNPLPMKSLTEKQTQQYESATNCYVCDSEFSVENYKVKDHCHVTQEFRGASCNNCNMQTKVPNFIPVIFHNLSGYDSHLFIKELGKNEEN--INVIPENTEKYISFSEYVSGKIFLRFIDSYRFMASSLDQLARNLT 530          
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: D7GXL4 (Rho_N domain-containing protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC006914 PE=4 SV=1)

HSP 1 Score: 293.123 bits (749), Expect = 1.208e-104
Identity = 177/458 (38.65%), Postives = 256/458 (55.90%), Query Frame = 3
Query: 1044 KAIFPYDFAKSLEVLESTTELPPI--------WGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYN-TLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILP--YGYTMEVDIEIPDNL--HDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            K I+PY++  S E     T+LP I           +S +E+  A ++W     + + ++   YLKLDV LL D F+ FR   +  +  L+P+ +Y  PG++W   L      +ELL D EM+ F+E GIRGG++  +  Y  A E+T+++YID NNLYGWALSQ LP  DF W+         E  NV  I +     GY +EVD+  P +L  H+  ND P+ PE   PP  KVEKL+   Y KKN+++H++ LKLY  LG  +  +HR IKF Q+     Y++ NT+ R   K +  +D +KL  N+ YGKS+++++ + N++L   +EK+    +KP F+   I   +L A H  K  +  ++P YIGQ VLDLSKL MY   Y  + K   EFN  + ++ GDTDSF  E+K+      ++    D   TS+Y   H LYS K   VIG FKDE
Sbjct:  775 KGIYPYEYMDSFEKF-FETKLPSIDKFYSSLTKEGISQEEFEHAVKVWKHFKIKNLGEWHDLYLKLDVLLLTDVFENFRDLCLEKSYGLDPVWYYTTPGLAWDCMLKYTSVELELLTDYEMYLFFEKGIRGGISQCSNRYSQADENTQIIYIDANNLYGWALSQKLPEKDFKWL------TCDEIKNVDGILVSDSDTGYVLEVDLAFPVDLAIHNKFNDLPLAPENRIPPGGKVEKLLCHFYDKKNYIIHYKTLKLYKSLGIRITKVHRGIKFTQSNFIAPYVKFNTEQRQKAKNDFEKDFWKLMVNAPYGKSMQSVRNQQNIKLYCDEEKLQKAINKPNFKHRTIFSKHLAAVHNFKTKVLFNKPIYIGQAVLDLSKLLMYNFHYNVIKK---EFNQNVKLLYGDTDSFIYEIKNADFYEFMKNN-PDYFDTSDYPKDHSLYSDKNKKVIGKFKDE 1221          

HSP 2 Score: 107.842 bits (268), Expect = 1.208e-104
Identity = 81/238 (34.03%), Postives = 117/238 (49.16%), Query Frame = 2
Query:  362 NYVKTVTPPFVIYADFESIL---------PLDIRHH--QIHMPIAAGLLLINNYTNTKQYYN-FIGDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKKFI---RDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINK-----LPVRFIDSMQFVNSSLAKAVKTLTDLPLTD 1015
            N  + +  P+VIY+DFESIL         P     H  Q H+P   GL + + Y N       + G++    F+K+I E   +I   Y  +   KPI  +   E  E++  + CYLC+        KV+DH H++GKY GAAC KCN+  R    +P++FHNL  YD H  +K  L    S +L +IP   EK+IS    +       + +RFIDS +F+ SSL      L    L +
Sbjct:  522 NIQRMLRHPYVIYSDFESILKPIPTCEPNPESSYTHKTQFHVPCGYGLYIKSAYNNIDNPLEIYRGEESDKRFIKRITEHTRTI---YKLLKENKPIKDI---ELEEYEKTKICYLCENVFSIENPKVRDHDHYTGKYRGAACRKCNLKYRQPLFIPILFHNLSSYDCHLFIK-NLGEDKS-QLKLIPNNEEKYISFGKILRMDDNGYIELRFIDSFRFLASSLDNLSSNLESSQLRE 751          

HSP 3 Score: 32.7278 bits (73), Expect = 1.208e-104
Identity = 22/68 (32.35%), Postives = 35/68 (51.47%), Query Frame = 2
Query: 2474 VVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWVGRNS-SLAFGHYL 2674
            V+  EI  + Y +C  N   +   Q  I    H +FT +  K +L+ K DKR  +  ++ +LA+GH L
Sbjct: 1257 VLKKEISFEDYKNCLFNQKEYFHTQKVIRSHKHDIFTEEVKKKSLSWKDDKRYLLPDSTDTLAYGHVL 1324          

HSP 4 Score: 21.9422 bits (45), Expect = 1.208e-104
Identity = 19/67 (28.36%), Postives = 30/67 (44.78%), Query Frame = 3
Query:  144 IYLPFLEDGDNYHYTSVFHLDKLMNCH-AITSCKIQI*VHWCPHCL-YGFRLKPKFDKHVSFWIINQ 338
            I L +  + DN HY  + +L +L+N    I   K     ++C  CL Y  R +    KH +    N+
Sbjct:  444 IDLLYFGNKDNNHYCWIKNLSRLLNDQLEINRSK----KYFCRRCLNYSARTEDALKKHQNVCFSNE 506          
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO46109 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR56])

HSP 1 Score: 257.299 bits (656), Expect = 2.038e-88
Identity = 160/470 (34.04%), Postives = 250/470 (53.19%), Query Frame = 3
Query: 1044 KAIFPYDFAKSLEVLESTTELPPIWG--------SVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASE------DTEL-----LYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKEL-AKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHP--LYSRKLDSVIGNFKDE*GS 2387
            K +FPYD+  + + L++  +LPP            +S ++Y  AQ++W      TM DY   YL+ DV LLAD F+ FR   +    L+P  +Y  PG++W +AL   +  +ELL D +M   +E GIRGG++ ++  +  A+       D  L      Y+D NNLYGWA+S+ LP   F W+    D+ L E           +   +EVD+  P +LHD  +D+P+ PE +    +KV KL+     K  +V+H   LKLY  LG +V  +HR I F+++   K YI  NT LR+    +  +D +KL NNS++GK++EN++ R+++RL  ++++     SKP ++   I  +NL A H+ K  +  ++P Y+G  +LDLSK  MY+  Y  +  KY D    +  ++  DTDS   E++       +   +R +  TSNY   HP  + S     +IG FKDE G 
Sbjct: 1063 KGVFPYDWFCNFDQLDAI-QLPPKEAFYSTLNDTDISEEDYQHAQKVWETFKMSTMRDYHDLYLESDVLLLADVFENFRDVCLENYGLDPAWYYTAPGLAWDAALKITRVELELLTDCDMLLMFEEGIRGGVSMISTRHSKANNPYMREYDPNLPTKYITYLDANNLYGWAMSKPLPTHGFRWM---TDQELKEWGR--------HPCVVEVDMAYPRHLHDSHDDYPLAPESI--KINKVGKLIPNLNDKTKYVVHHETLKLYESLGLKVTKVHRGITFEESGWLKTYIDLNTSLRAKATNDFEKDFFKLMNNSVFGKTMENIRNRVDIRLITNEKQARKLISKPNYKHRTIFCENLAAIHMRKTRLVFNKPVYLGMCILDLSKNLMYDFHYGYVKPKYGD----KAKLLFTDTDSLMYEIETEDFYKDISGDVRSMFDTSNYPRNHPSGIESGLNKKIIGMFKDETGG 1514          

HSP 2 Score: 88.9669 bits (219), Expect = 2.038e-88
Identity = 73/244 (29.92%), Postives = 113/244 (46.31%), Query Frame = 2
Query:  362 NYVKTVTPPFVIYADFESIL-------PLDIR----HHQIHMPIAAGLLLINNYTN-------TKQYYNFIGDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCK-KFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYI--------------NKLPVRFIDSMQFVNSSLAKAVKTL 994
            N+ + +  PFV+YADFES         P + R     +Q H P     L+  +  +        ++      +D    F++ ++     I   Y K    K +  ++ +++  + +   C++C  +   DKV DHCH +GKY GAA   CN+  R  K  PV+FHNL GYD H  +K  L   S  ++N IP   EK+IS    +               K  +RFIDS +F+++SL   VK L
Sbjct:  803 NFNRKMRVPFVVYADFESFTENIDTCSPDESRSFTKQYQKHKPSGFCYLIKCSIDDGVFSPKLVQRTVQGSAEDVAQLFVESLESDIKEI---YNKFKFPKEV-EMTREDEMNYDNATHCHICGGELGEDKVLDHCHLTGKYRGAAHNACNLRFRAPKFFPVLFHNLSGYDSHLFVK-NLGT-SEGKINCIPNNEEKYISFTKQVVVDRFTNKEGKQVDVKRDIRFIDSFKFMSASLDSLVKNL 1040          
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO45323 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT38])

HSP 1 Score: 84.3445 bits (207), Expect = 2.452e-17
Identity = 80/276 (28.99%), Postives = 125/276 (45.29%), Query Frame = 3
Query: 1617 EVDIEIPDNLHDFLNDF-PI-----GPEKMCPPNSKV------------EKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLR----SNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            +VDI  P+NL +   +F P+      PE++ P + K              K +L     K  +L+  LLK Y+  G EV  IH+ IK+     FK +    ++ R    SN   +   D YKL  NS YGK +E+L+K         DE  +  A +  F   + + ++       K  + ++RP   G  V  L+KLRM E  Y  L KY D  + E  ++  DTDS ++ + +  LD +++  ++D  Y+ N        S+  D   G FK E
Sbjct:    3 QVDIHAPENLREKFEEFSPLFVVDSVPEELVPEHMKQYQKDTDRKTLKGTKKLLGVTKAKKILLYTPLLKWYLTHGLEVTAIHKIIKYVSGRPFKWFPDEVSNARRDGDSNPALKQLGDTYKLKGNSFYGKMIEDLEKHTKTSYTQ-DEAEVDKAFRSPFFDDLEVINDTYELKERKRQVTINRPYQCGIAVYQLAKLRMLEFYYDFLDKYVDRKDFE--LIQMDTDSLYMALSNENLDEIIKPKLQD-EYSQNGKAEFLSTSKYHDRTPGLFKLE 274          
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000066165.1 (SMESG000066165.1)

HSP 1 Score: 826.624 bits (2134), Expect = 0.000e+0
Identity = 396/448 (88.39%), Postives = 417/448 (93.08%), Query Frame = 3
Query: 1038 RAKAIFPYDFAKSLEVLESTTELPPIWGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRD-LLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            +AK IFPYDFA SL+VLEST ELPP W SVS DEYATAQ IWTQQNC TMLDYMLTYLKLDVFLLADYFQQFRAKAIAYN LEPLNFYGIPGMSWASALMTL+EPIELLQDMEMFNFYEGGIRGGLTFVNKH++A+SEDTELLYIDINNLYGWALSQ+LPY DF WV+D L++VLSEC N  +IE LPYGYTMEVDI IP+ +H +LNDFP+ PEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQ+PIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNS EKMIVYASKPTF+KSI I D+LIA HLNKDSICLDRPSYIGQ+VLDLSKLRMY+LQY ELAKYR EFNC+INIVAGDTDSFFLEVKHCKLD LL AMIRD LL TSNYDPAHPLYSRKLDSVIG FKDE
Sbjct:  528 KAKGIFPYDFAVSLKVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNA-NIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQSPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDSVIGKFKDE 974          

HSP 2 Score: 258.07 bits (658), Expect = 0.000e+0
Identity = 124/171 (72.51%), Postives = 145/171 (84.80%), Query Frame = 2
Query:  548 KKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRFIDSMQFVNSSLAKAVKTLTDLPLTDSVFDGAI-*SQGYFP 1057
            KK++EIA++IVLPYY+ H +KP+  L+  E+++F  CRTCYLCKK IR+KVKDH HF+G+YLGAAC  CN++R+I KQLP+VFHNLRGYDLHHILKYGLN F SW+LNIIPTTTEKFISLI  I KL VRFIDSMQFVN SLAKAVKTLTDLPLT S FDG I  ++G FP
Sbjct:  364 KKVEEIAVTIVLPYYENHGKKPMNNLTSNEEQQFIACRTCYLCKKLIRNKVKDHDHFTGQYLGAACRNCNINRKISKQLPIVFHNLRGYDLHHILKYGLNEFPSWKLNIIPTTTEKFISLIVNIKKLAVRFIDSMQFVNCSLAKAVKTLTDLPLTCSAFDGLIVKAKGIFP 534          

HSP 3 Score: 123.635 bits (309), Expect = 2.792e-35
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 2
Query: 2465 GNEVVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWVGRNSSLAFGHYLGVDVI--DDE*VTHSPRYESTHSPSYD 2749
            G +++GT+I+HQSYLDC+N +++FTVPQ+RIG RNHQL+TFKNSKIAL+N  DKR WVG+N+S+AFGHY GVD I  D       P +  + SPS+D
Sbjct: 1004 GVKLLGTDIKHQSYLDCYNKNVVFTVPQSRIGTRNHQLYTFKNSKIALSNNDDKRLWVGKNTSIAFGHYFGVDAIVSDQVNFLDEPLFGGS-SPSFD 1099          

HSP 4 Score: 45.8246 bits (107), Expect = 2.792e-35
Identity = 21/30 (70.00%), Postives = 21/30 (70.00%), Query Frame = 1
Query: 2377 SKDLGYAEWVFLRLKFYSLLNTKDTMRVNG 2466
            SK LGY EWVFLR K YSLL   DTMR  G
Sbjct:  975 SKGLGYKEWVFLRPKCYSLLGKSDTMRAKG 1004          
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000066165.1 (SMESG000066165.1)

HSP 1 Score: 826.239 bits (2133), Expect = 0.000e+0
Identity = 396/448 (88.39%), Postives = 417/448 (93.08%), Query Frame = 3
Query: 1038 RAKAIFPYDFAKSLEVLESTTELPPIWGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRD-LLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            +AK IFPYDFA SL+VLEST ELPP W SVS DEYATAQ IWTQQNC TMLDYMLTYLKLDVFLLADYFQQFRAKAIAYN LEPLNFYGIPGMSWASALMTL+EPIELLQDMEMFNFYEGGIRGGLTFVNKH++A+SEDTELLYIDINNLYGWALSQ+LPY DF WV+D L++VLSEC N  +IE LPYGYTMEVDI IP+ +H +LNDFP+ PEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQ+PIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNS EKMIVYASKPTF+KSI I D+LIA HLNKDSICLDRPSYIGQ+VLDLSKLRMY+LQY ELAKYR EFNC+INIVAGDTDSFFLEVKHCKLD LL AMIRD LL TSNYDPAHPLYSRKLDSVIG FKDE
Sbjct:  590 KAKGIFPYDFAVSLKVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNA-NIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQSPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDSVIGKFKDE 1036          

HSP 2 Score: 270.011 bits (689), Expect = 0.000e+0
Identity = 130/180 (72.22%), Postives = 152/180 (84.44%), Query Frame = 2
Query:  521 GDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRFIDSMQFVNSSLAKAVKTLTDLPLTDSVFDGAI-*SQGYFP 1057
            G +CVL FLKK++EIA++IVLPYY+ H +KP+  L+  E+++F  CRTCYLCKK IR+KVKDH HF+G+YLGAAC  CN++R+I KQLP+VFHNLRGYDLHHILKYGLN F SW+LNIIPTTTEKFISLI  I KL VRFIDSMQFVN SLAKAVKTLTDLPLT S FDG I  ++G FP
Sbjct:  417 GLECVLEFLKKVEEIAVTIVLPYYENHGKKPMNNLTSNEEQQFIACRTCYLCKKLIRNKVKDHDHFTGQYLGAACRNCNINRKISKQLPIVFHNLRGYDLHHILKYGLNEFPSWKLNIIPTTTEKFISLIVNIKKLAVRFIDSMQFVNCSLAKAVKTLTDLPLTCSAFDGLIVKAKGIFP 596          

HSP 3 Score: 123.635 bits (309), Expect = 2.933e-35
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 2
Query: 2465 GNEVVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWVGRNSSLAFGHYLGVDVI--DDE*VTHSPRYESTHSPSYD 2749
            G +++GT+I+HQSYLDC+N +++FTVPQ+RIG RNHQL+TFKNSKIAL+N  DKR WVG+N+S+AFGHY GVD I  D       P +  + SPS+D
Sbjct: 1066 GVKLLGTDIKHQSYLDCYNKNVVFTVPQSRIGTRNHQLYTFKNSKIALSNNDDKRLWVGKNTSIAFGHYFGVDAIVSDQVNFLDEPLFGGS-SPSFD 1161          

HSP 4 Score: 45.8246 bits (107), Expect = 2.933e-35
Identity = 21/30 (70.00%), Postives = 21/30 (70.00%), Query Frame = 1
Query: 2377 SKDLGYAEWVFLRLKFYSLLNTKDTMRVNG 2466
            SK LGY EWVFLR K YSLL   DTMR  G
Sbjct: 1037 SKGLGYKEWVFLRPKCYSLLGKSDTMRAKG 1066          

HSP 5 Score: 71.633 bits (174), Expect = 3.155e-12
Identity = 31/70 (44.29%), Postives = 45/70 (64.29%), Query Frame = 3
Query:  108 IIYCFKFTVGTAIYLPFLEDGDNYHYTSVFHLDKLMNCHAITSCKIQI*VHWCPHCLYGFRLKPKFDKHV 317
            +I+  K   G  I L  L+DGDN+HYT++F+L++L+NC + + C  +I V WCPHCL G     +F K V
Sbjct:  359 LIHRTKVIGGNPINLLLLQDGDNFHYTAIFNLNRLLNCRSSSECNTRIHVKWCPHCLNGLECVLEFLKKV 428          
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000066165.1 (SMESG000066165.1)

HSP 1 Score: 825.854 bits (2132), Expect = 0.000e+0
Identity = 396/448 (88.39%), Postives = 417/448 (93.08%), Query Frame = 3
Query: 1038 RAKAIFPYDFAKSLEVLESTTELPPIWGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRD-LLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            +AK IFPYDFA SL+VLEST ELPP W SVS DEYATAQ IWTQQNC TMLDYMLTYLKLDVFLLADYFQQFRAKAIAYN LEPLNFYGIPGMSWASALMTL+EPIELLQDMEMFNFYEGGIRGGLTFVNKH++A+SEDTELLYIDINNLYGWALSQ+LPY DF WV+D L++VLSEC N  +IE LPYGYTMEVDI IP+ +H +LNDFP+ PEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQ+PIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNS EKMIVYASKPTF+KSI I D+LIA HLNKDSICLDRPSYIGQ+VLDLSKLRMY+LQY ELAKYR EFNC+INIVAGDTDSFFLEVKHCKLD LL AMIRD LL TSNYDPAHPLYSRKLDSVIG FKDE
Sbjct:  552 KAKGIFPYDFAVSLKVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLYIDINNLYGWALSQNLPYGDFVWVHDNLNDVLSECVNA-NIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQSPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRPSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDNLLDTSNYDPAHPLYSRKLDSVIGKFKDE 998          

HSP 2 Score: 270.396 bits (690), Expect = 0.000e+0
Identity = 130/180 (72.22%), Postives = 152/180 (84.44%), Query Frame = 2
Query:  521 GDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRFIDSMQFVNSSLAKAVKTLTDLPLTDSVFDGAI-*SQGYFP 1057
            G +CVL FLKK++EIA++IVLPYY+ H +KP+  L+  E+++F  CRTCYLCKK IR+KVKDH HF+G+YLGAAC  CN++R+I KQLP+VFHNLRGYDLHHILKYGLN F SW+LNIIPTTTEKFISLI  I KL VRFIDSMQFVN SLAKAVKTLTDLPLT S FDG I  ++G FP
Sbjct:  379 GLECVLEFLKKVEEIAVTIVLPYYENHGKKPMNNLTSNEEQQFIACRTCYLCKKLIRNKVKDHDHFTGQYLGAACRNCNINRKISKQLPIVFHNLRGYDLHHILKYGLNEFPSWKLNIIPTTTEKFISLIVNIKKLAVRFIDSMQFVNCSLAKAVKTLTDLPLTCSAFDGLIVKAKGIFP 558          

HSP 3 Score: 123.635 bits (309), Expect = 2.909e-35
Identity = 58/97 (59.79%), Postives = 76/97 (78.35%), Query Frame = 2
Query: 2465 GNEVVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWVGRNSSLAFGHYLGVDVI--DDE*VTHSPRYESTHSPSYD 2749
            G +++GT+I+HQSYLDC+N +++FTVPQ+RIG RNHQL+TFKNSKIAL+N  DKR WVG+N+S+AFGHY GVD I  D       P +  + SPS+D
Sbjct: 1028 GVKLLGTDIKHQSYLDCYNKNVVFTVPQSRIGTRNHQLYTFKNSKIALSNNDDKRLWVGKNTSIAFGHYFGVDAIVSDQVNFLDEPLFGGS-SPSFD 1123          

HSP 4 Score: 45.8246 bits (107), Expect = 2.909e-35
Identity = 21/30 (70.00%), Postives = 21/30 (70.00%), Query Frame = 1
Query: 2377 SKDLGYAEWVFLRLKFYSLLNTKDTMRVNG 2466
            SK LGY EWVFLR K YSLL   DTMR  G
Sbjct:  999 SKGLGYKEWVFLRPKCYSLLGKSDTMRAKG 1028          
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000037212.1 (SMESG000037212.1)

HSP 1 Score: 796.579 bits (2056), Expect = 0.000e+0
Identity = 384/447 (85.91%), Postives = 408/447 (91.28%), Query Frame = 3
Query: 1038 RAKAIFPYDFAKSLEVLESTTELPPIWGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            +AK IFPYDFA SLEVLEST ELPP W SVS DEYATAQ IWTQQNC TMLDYMLTYLKLDVFLLADYFQQFRAKAIAYN LEPLNFYGIPGMSWASALMTL+EPIELLQDMEMFNFYEGGIRGGLTFVNKH++A+SEDTELL+IDINNLYGWALSQ+LPY DF WV+  L++VLSEC N  +IE LPYGYTMEVDI IP+ +H +LNDFP+ PEKMCPPNSKVEKLMLTHYSKKNHVLHW+LLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSN+KCEL+RDLYKLFNNSLYGKSVENLKKRMNLRLCNS EKMIVYASKPTF+KSI I D+LIA HLNKDSICLDR SYIGQ+VLDLSKLRMY+LQY ELAKYR EFNC+INIVAGDTDSFFLEVKHCKLD LL AMIRD L  +     HPLYSRKLDSVIG FKDE
Sbjct: 1145 KAKGIFPYDFAVSLEVLESTYELPPKWDSVSDDEYATAQLIWTQQNCTTMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNGLEPLNFYGIPGMSWASALMTLKEPIELLQDMEMFNFYEGGIRGGLTFVNKHHVASSEDTELLHIDINNLYGWALSQNLPYGDFVWVHYNLNDVLSECVNA-NIEFLPYGYTMEVDINIPERVHQYLNDFPLAPEKMCPPNSKVEKLMLTHYSKKNHVLHWKLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNSKCELHRDLYKLFNNSLYGKSVENLKKRMNLRLCNSAEKMIVYASKPTFKKSIKIVDDLIAAHLNKDSICLDRLSYIGQSVLDLSKLRMYQLQYNELAKYRSEFNCDINIVAGDTDSFFLEVKHCKLDILLPAMIRDSLLDT-----HPLYSRKLDSVIGKFKDE 1585          

HSP 2 Score: 126.716 bits (317), Expect = 3.360e-29
Identity = 59/97 (60.82%), Postives = 78/97 (80.41%), Query Frame = 2
Query: 2465 GNEVVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRNWVGRNSSLAFGHYLGVDVIDDE*VT--HSPRYESTHSPSYD 2749
            G +++GT+I+HQSYLDC+N +++FTVPQ+RIG RNHQL+TFKNSKIAL+N  DKR WVG+N+S+AFGHY GVD ID + V     P +  + SPS+D
Sbjct: 1595 GVKLLGTDIKHQSYLDCYNKNVVFTVPQSRIGTRNHQLYTFKNSKIALSNNDDKRLWVGKNTSIAFGHYFGVDAIDSDQVNFLDEPLFGGS-SPSFD 1690          

HSP 3 Score: 67.3958 bits (163), Expect = 5.457e-11
Identity = 37/48 (77.08%), Postives = 39/48 (81.25%), Query Frame = 2
Query:  917 INKLPVRFIDSMQFVNSSLAKAVKTLTDLPLTDSVFDGAI-*SQGYFP 1057
            I KL VRFIDSMQFVN SLAKAVKTLTDLPLT S FDG I  ++G FP
Sbjct: 1104 IRKLAVRFIDSMQFVNCSLAKAVKTLTDLPLTCSAFDGLIVKAKGIFP 1151          

HSP 4 Score: 51.9878 bits (123), Expect = 2.584e-6
Identity = 21/48 (43.75%), Postives = 33/48 (68.75%), Query Frame = 3
Query:  108 IIYCFKFTVGTAIYLPFLEDGDNYHYTSVFHLDKLMNCHAITSCKIQI 251
            +I+  K   G  I L  L+DGDNYHYT++F+L++ +NCH+ + C  +I
Sbjct: 1057 LIHRTKVIGGNPINLLLLQDGDNYHYTAIFNLNRQLNCHSSSECNTRI 1104          
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000062279.1 (SMESG000062279.1)

HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0
Identity = 385/448 (85.94%), Postives = 402/448 (89.73%), Query Frame = 3
Query: 1038 RAKAIFPYDFAKSLEVLESTTELPPIWGSVSADEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWASALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYIDINNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVDIEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKLYIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFNNSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLRAMIRD-LLYTSNYDPAHPLYSRKLDSVIGNFKDE 2378
            RAKAIFPYDFAKSLEVLESTTELPPIWGSVS DEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNF                  +E L DMEMFNFYEGGIRGGLTFVNKHY+A+ EDTE LYIDINNLYGWALSQ LPYADF WVYDGLD+VL+EC NV DIEILPYGYTMEVDI+IPD LH+FLNDFPIGPEKMCPPNSKVEKLMLTH+ KKNHVLHWRLL+LY+ LG  V  +HRAIKFKQAPIF  YI +NT LR+ +  ELYR+L+KL+NNSLYGKSVENLKK MNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLL AMIRD LL TSNYDPAHPLYSRKLDSVIG FKDE
Sbjct: 1571 RAKAIFPYDFAKSLEVLESTTELPPIWGSVSVDEYATAQQIWTQQNCETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNF------------------MEFL-DMEMFNFYEGGIRGGLTFVNKHYVASFEDTEFLYIDINNLYGWALSQYLPYADFVWVYDGLDDVLNECMNVADIEILPYGYTMEVDIDIPDYLHNFLNDFPIGPEKMCPPNSKVEKLMLTHWPKKNHVLHWRLLRLYLSLGVNVVKVHRAIKFKQAPIFHAYIDKNTKLRAQSTSELYRNLFKLYNNSLYGKSVENLKKWMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHLNKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGDTDSFFLEVKHCKLDTLLPAMIRDSLLDTSNYDPAHPLYSRKLDSVIGKFKDE 1999          

HSP 2 Score: 462.225 bits (1188), Expect = 0.000e+0
Identity = 217/225 (96.44%), Postives = 223/225 (99.11%), Query Frame = 2
Query:  362 NYVKTVTPPFVIYADFESILPLDIRHHQIHMPIAAGLLLINNYTNTKQYYNFIGDDCVLAFLKKIDEIALSIVLPYYKVHCQKPIGPLSFKEKREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRFIDSMQFVNSSLAKAVKTLTDLPLTDSVFDGAI 1036
            N+ K++TPPFVIYADFESILPLDIRHHQIHMPIAAGLLLINNYTNTKQY+NFIGDDCVLAFLKKIDEIALSIVLPYY+VHCQKPIGPLSFKE REFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRFIDSMQF+NSSLAKAVKTLTDLPLTDSVFDGAI
Sbjct: 1345 NFAKSITPPFVIYADFESILPLDIRHHQIHMPIAAGLLLINNYTNTKQYFNFIGDDCVLAFLKKIDEIALSIVLPYYEVHCQKPIGPLSFKEDREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMKCNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFISLIAYINKLPVRFIDSMQFINSSLAKAVKTLTDLPLTDSVFDGAI 1569          

HSP 3 Score: 97.4413 bits (241), Expect = 6.858e-28
Identity = 45/56 (80.36%), Postives = 50/56 (89.29%), Query Frame = 2
Query: 2465 GNEVVGTEIEHQSYLDCFNNDIIFTVPQTRIGMRNHQLFTFKNSKIALTNKVDKRN 2632
            G +++GTEI+HQSYLDCF ND IFTVPQTRIG RNHQLFTFKN+KIALTN  DKRN
Sbjct: 2029 GVKLLGTEIDHQSYLDCFENDTIFTVPQTRIGTRNHQLFTFKNNKIALTNNDDKRN 2084          

HSP 4 Score: 47.3654 bits (111), Expect = 6.858e-28
Identity = 22/30 (73.33%), Postives = 23/30 (76.67%), Query Frame = 1
Query: 2377 SKDLGYAEWVFLRLKFYSLLNTKDTMRVNG 2466
            SK LGY EWVFLR K YSLL TKD+MR  G
Sbjct: 2000 SKGLGYKEWVFLRPKCYSLLGTKDSMRAKG 2029          

HSP 5 Score: 122.479 bits (306), Expect = 8.784e-28
Identity = 57/81 (70.37%), Postives = 62/81 (76.54%), Query Frame = 3
Query:  102 FDIIYCFKFTVGTAIYLPFLEDGDNYHYTSVFHLDKLMNCHAITSCKIQI*VHWCPHCLYGFRLKPKFDKHVSFWIINQTG 344
             DII+  K T GT IYL  LEDGDNYHYT+VF L+KLMNCHA TSC +QI VHWCPHCL GFRLKP FD HV+    NQTG
Sbjct: 1249 LDIIHRSKVTDGTPIYLLLLEDGDNYHYTAVFDLNKLMNCHATTSCNVQISVHWCPHCLNGFRLKPAFDAHVALCSKNQTG 1329          
The following BLAST results are available for this feature:
BLAST of Rho_N domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ZC53.41.761e-4735.05pep chromosome:WBcel235:X:1925750:1937689:1 gene:W... [more]
H12I13.31.131e-2328.85pep chromosome:WBcel235:II:6071386:6073251:-1 gene... [more]
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BLAST of Rho_N domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2L2YHT11.406e-10536.67Uncharacterized protein (Fragment) OS=Parasteatoda... [more]
K7JB672.943e-10535.86Uncharacterized protein OS=Nasonia vitripennis OX=... [more]
A0A224XH846.819e-10538.35Putative dna polymerase type b (Fragment) OS=Panst... [more]
A0A2L2YHS07.599e-10536.32Uncharacterized protein (Fragment) OS=Parasteatoda... [more]
D7GXL41.208e-10438.65Rho_N domain-containing protein OS=Tribolium casta... [more]
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BLAST of Rho_N domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO461092.038e-8834.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO453232.452e-1728.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Rho_N domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000066165.12.792e-3588.39SMESG000066165.1[more]
SMESG000066165.12.933e-3588.39SMESG000066165.1[more]
SMESG000066165.12.909e-3588.39SMESG000066165.1[more]
SMESG000037212.13.360e-2985.91SMESG000037212.1[more]
SMESG000062279.16.858e-2885.94SMESG000062279.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030071 ID=SMED30030071|Name=Rho_N domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2908bp
GCCTAAGGTAGGTAATAGTCATTCCTAAGAGCAATCCATCTCCTGCACTC
TGAAGCCTTCATAAAAAAAAATAAAATAATAATAATTTGGAAGAATTGAA
TTTTGATATAATTTACTGCTTCAAGTTTACTGTCGGTACAGCTATTTATT
TACCTTTTTTGGAGGACGGTGATAATTATCACTATACGTCTGTTTTTCAT
TTGGATAAGTTAATGAACTGTCATGCGATCACATCTTGCAAAATCCAAAT
CTAGGTACATTGGTGTCCCCATTGTCTTTATGGCTTTAGATTAAAACCGA
AATTTGACAAACATGTTTCCTTTTGGATTATAAATCAAACAGGTAAAAAA
TAAATATTTAAAATTATGTGAAAACTGTTACCCCGCCATTCGTAATTTAT
GCGGATTTTGAGTCAATTTTACCACTGGATATAAGGCATCATCAAATACA
TATGCCGATAGCAGCAGGTCTATTATTAATTAACAACTACACAAACACAA
AACAATATTATAATTTTATTGGCGATGATTGCGTTTTGGCGTTTTTAAAA
AAGATTGATGAAATCGCTCTTTCGATTGTCTTGCCATATTACAAGGTTCA
TTGTCAAAAACCTATAGGACCACTATCTTTCAAGGAGAAACGAGAGTTCA
AGGATTGTCGCACTTGTTATTTGTGTAAAAAGTTTATTAGGGATAAAGTT
AAGGATCATTGTCATTTCTCAGGTAAGTATTTAGGGGCTGCTTGTATGAA
GTGTAATGTTTCTCGACGAATCCAGAAACAATTACCTGTGGTTTTCCATA
ATTTGAGGGGATATGATCTCCATCATATTTTAAAGTATGGTCTAAATAAT
TTTTCTTCTTGGGAATTAAATATTATTCCGACCACTACCGAAAAATTTAT
CTCCCTCATCGCATACATTAATAAATTGCCTGTTCGTTTTATAGACAGCA
TGCAATTTGTTAATTCATCTTTAGCAAAAGCGGTTAAGACACTTACTGAT
TTGCCTTTAACTGATTCTGTTTTTGATGGTGCAATTTAGAGCCAAGGCTA
TTTTCCCTTATGACTTTGCGAAATCTCTTGAGGTTCTTGAATCGACGACT
GAATTGCCTCCTATTTGGGGTTCTGTATCTGCTGATGAATACGCTACAGC
CCAACAAATTTGGACACAGCAAAATTGCGAGACAATGCTAGATTACATGT
TAACCTACTTAAAATTAGACGTATTCCTTCTTGCGGATTATTTTCAGCAA
TTTCGCGCAAAAGCAATTGCTTATAATACTCTAGAACCTCTTAATTTTTA
TGGAATTCCTGGTATGTCATGGGCGTCAGCTCTCATGACATTACAGGAAC
CCATAGAACTTTTGCAGGACATGGAAATGTTTAATTTCTATGAGGGTGGA
ATACGGGGTGGGTTAACATTTGTTAACAAACATTACATTGCTGCTTCGGA
GGATACAGAATTATTGTACATAGATATAAATAATCTGTATGGTTGGGCTC
TCAGCCAAAGTCTTCCTTACGCTGATTTTACTTGGGTGTATGATGGTTTG
GATGAGGTTTTGAGTGAATGTGCGAATGTTGTGGATATTGAAATCCTTCC
ATATGGATATACTATGGAAGTTGATATCGAGATTCCAGATAATTTACATG
ATTTTTTAAATGACTTTCCGATTGGACCGGAAAAGATGTGCCCACCAAAT
TCAAAAGTTGAGAAGTTAATGCTCACGCATTACAGTAAGAAGAACCATGT
TCTTCACTGGAGACTTCTCAAGCTGTATATAGAATTGGGTGCAGAAGTTG
AGTGTATTCATAGGGCAATTAAATTTAAACAAGCACCTATTTTTAAAGAA
TATATACAAAGAAATACAGATTTACGCTCAAACACAAAGTGCGAGCTTTA
TAGAGATTTGTATAAATTATTTAATAATAGCCTATATGGGAAGTCCGTTG
AGAATTTGAAAAAACGGATGAATTTGAGGCTATGCAATTCTGATGAAAAG
ATGATTGTATATGCCTCAAAACCGACTTTCAGAAAATCTATCATAATTGA
TGATAATCTGATTGCTACTCATCTGAATAAGGATTCGATATGTTTAGACA
GGCCTAGTTATATCGGACAAACAGTTCTAGACCTGTCTAAACTTAGAATG
TACGAACTACAATATAAAGAGCTAGCGAAATATCGCGATGAATTTAATTG
TGAAATAAATATCGTTGCCGGCGACACTGATTCGTTTTTCCTTGAGGTTA
AGCACTGTAAGCTAGACACTCTTTTGCGGGCAATGATTAGAGACCTTTTA
TATACGTCCAATTACGACCCGGCACATCCCTTGTACTCAAGAAAGCTTGA
CTCTGTAATAGGAAATTTTAAAGATGAGTAAGGATCTTGGGTATGCGGAA
TGGGTTTTCTTAAGACTCAAGTTCTATAGTTTGTTAAATACTAAAGATAC
GATGAGGGTTAATGGGGAATGAAGTTGTTGGGACAGAGATTGAGCACCAG
TCATATTTAGATTGTTTTAATAATGACATAATATTCACGGTGCCTCAAAC
TAGGATAGGGATGCGGAACCATCAGTTGTTCACATTTAAAAACTCTAAAA
TTGCTCTTACCAATAAAGTCGATAAGCGTAATTGGGTGGGTAGAAATTCC
AGTCTCGCATTTGGCCATTATTTAGGAGTCGATGTCATTGATGATGAATA
AGTAACGCACAGTCCAAGATATGAGAGCACGCACAGCCCCAGTTATGATA
GCTTTTATCATAATAGCTCTTACAGTCAAGATGTCGATAGTGACGATGTA
CTTTTATCAGACTGTATCTTGTGATTTATTGCAAAATAATGTATTTTTCT
TGAATTTCTTGAGATTTTATCTATTTATGTTTTATCAACTTTTTATTGTA
TAATAGGA
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protein sequence of SMED30030071-orf-1

>SMED30030071-orf-1 ID=SMED30030071-orf-1|Name=SMED30030071-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=196bp
MPIAAGLLLINNYTNTKQYYNFIGDDCVLAFLKKIDEIALSIVLPYYKVH
CQKPIGPLSFKEKREFKDCRTCYLCKKFIRDKVKDHCHFSGKYLGAACMK
CNVSRRIQKQLPVVFHNLRGYDLHHILKYGLNNFSSWELNIIPTTTEKFI
SLIAYINKLPVRFIDSMQFVNSSLAKAVKTLTDLPLTDSVFDGAI*
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protein sequence of SMED30030071-orf-2

>SMED30030071-orf-2 ID=SMED30030071-orf-2|Name=SMED30030071-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=452bp
MVQFRAKAIFPYDFAKSLEVLESTTELPPIWGSVSADEYATAQQIWTQQN
CETMLDYMLTYLKLDVFLLADYFQQFRAKAIAYNTLEPLNFYGIPGMSWA
SALMTLQEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAASEDTELLYID
INNLYGWALSQSLPYADFTWVYDGLDEVLSECANVVDIEILPYGYTMEVD
IEIPDNLHDFLNDFPIGPEKMCPPNSKVEKLMLTHYSKKNHVLHWRLLKL
YIELGAEVECIHRAIKFKQAPIFKEYIQRNTDLRSNTKCELYRDLYKLFN
NSLYGKSVENLKKRMNLRLCNSDEKMIVYASKPTFRKSIIIDDNLIATHL
NKDSICLDRPSYIGQTVLDLSKLRMYELQYKELAKYRDEFNCEINIVAGD
TDSFFLEVKHCKLDTLLRAMIRDLLYTSNYDPAHPLYSRKLDSVIGNFKD
E*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006353DNA-templated transcription, termination
GO:0006260DNA replication
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000136whole organism
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
IPR023211DNA_pol_palm_dom_sf
IPR004868DNA-dir_DNA_pol_B_mt/vir
IPR017964DNA-dir_DNA_pol_B_CS
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:00084083'-5' exonuclease activity
GO:0000166nucleotide binding
GO:0003677DNA binding
GO:0003887DNA-directed DNA polymerase activity