SMED30030030

Overview
NameSMED30030030
Smed IDSMED30030030
Length (bp)1476
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30030030 (SMED30030030) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30030030 (SMED30030030) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30030030 (SMED30030030) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30030030 (SMED30030030) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30030030

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30030030SMESG000007276.1 SmedASXL_009711SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30030030SMESG000007276.1 dd_Smed_v4_11992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30030030SMESG000007276.1 dd_Smed_v4_11992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30030030SMESG000007276.1 dd_Smed_v4_11992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30030030SMESG000007276.1 dd_Smed_v6_11992_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30030030SMESG000007276.1 OX_Smed_1.0.02193ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30030030SMESG000061623.1 SMESG000007276.1 SMESG000048027.1 SMESG000023758.1 Contig16923newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30030030SMESG000061623.1 SMESG000007276.1 SMESG000048027.1 SMESG000023758.1 Contig16923uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30030030SMESG000007276.1 dd_Smed_v4_11992_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30030030 vs. TrEMBL
Match: A0A1I8GGD4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032595g1 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.067e-22
Identity = 114/386 (29.53%), Postives = 182/386 (47.15%), Query Frame = 1
Query:  199 KNKPLKLHTPKVRLRLQEAC--DNSDKAKISTVK--YCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKI-CQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRN-RGSLLIVGSGEGKRIPEEI-HIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVR---DDEATEISKRNHNGSMSDVMNQKQQKYPVVLREP----RCPTAESLE-TWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCP 1311
            ++KP+K+H+PKVR    +A    N ++++ S VK  +   P++++     + +   I    +E  I R +   +MDK++ P     YQ +   P+C  E+S  +  RN RG+   +         +E   +RCPS  A +NL KGRG  M   L H  +   +PP P +R     E+ E   R+  G MS V NQ +       ++P    R    E+ E    ++RG +  +    +    D      P  RV+ E   +      + M            + P+  PRVRL G + A K +GTLT+E YNY ++       P P+V  + +E A+  +  +TK L++KYGQ P D  PKP V   +   A +S+G AM+D + P
Sbjct:   59 RSKPIKVHSPKVRTGWDKAAVVANGERSRSSAVKANFDYRPENAERTEKQQPRGSAIDGRFAE--IQRKQTHTEMDKVLNPSKERFYQPEM-NPKCPTEDSKEFYRRNQRGTYGFMEPPRANYPTDEPGKVRCPSAAARENLEKGRG--MAGRLIHQEERSRTPP-PSLRLGASQESRENYDRSRGGCMSAVFNQSENIR-AGAQDPAPAGRVIKGEAAEQNASRNRGQTAQLFAPGRGAQPD----PQPAPRVRPE-AQETAAREGAGMRGTF---YGGPQTEPQPAPRVRLGGGEVASKLQGTLTSEQYNYRQMP--PDPPPAPKVRPEAQEYANRERAVVTKNLLQKYGQLPSDLPPKPHVPPQAWNTAYRSRGSAMKDCINP 427          
BLAST of SMED30030030 vs. TrEMBL
Match: A0A267H1G3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009461g1 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 1.542e-21
Identity = 114/386 (29.53%), Postives = 181/386 (46.89%), Query Frame = 1
Query:  199 KNKPLKLHTPKVRLRLQEAC--DNSDKAKISTVK--YCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKI-CQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRN-RGSLLIVGSGEGKRIPEEI-HIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVR---DDEATEISKRNHNGSMSDVMNQKQQKYPVVLREP----RCPTAESLE-TWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCP 1311
            ++KP+K+H+PKVR    +A    N ++++ S VK  +   P++++     + +   I    +E  I R +   +MDK++ P     YQ +   P+C  E+S  +  RN RG+   +         +E   +RCPS  A +NL KGRG  M   L H  +   +PP P +R     E  E   R+  G MS V NQ +       ++P    R    E+ E    ++RG +  +    +    D      P  RV+ E   +      + M            + P+  PRVRL G + A K +GTLT+E YNY ++       P P+V  + +E A+  +  +TK L++KYGQ P D  PKP V   +   A +S+G AM+D + P
Sbjct:   59 RSKPIKVHSPKVRTGWDKAAVVANGERSRSSAVKANFDYRPENAERTEKQQPRGSAIDGRFAE--IQRKQTHTEMDKVLNPSKERFYQPEM-NPKCPTEDSKEFCRRNQRGTYGFMEPLRANYPTDEPGKVRCPSAAARENLEKGRG--MAGRLIHQEERSRTPP-PSLRLGASQETRENYDRSRGGCMSAVFNQSENIR-AGAQDPAPAGRVIKGEAAEQNASRNRGQTAQLFAPGRGAQPD----PQPAPRVRPE-AQETAAREGAGMRGTF---YGGPQTEPQPAPRVRLGGGEVASKLQGTLTSEQYNYRQMP--PDPPPAPKVRPEAQEYANRERAVVTKNLLQKYGQLPSDLPPKPHVPPQAWNTAYRSRGSAMKDCINP 427          
BLAST of SMED30030030 vs. TrEMBL
Match: A0A4Z2DCD8 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002403 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.611e-14
Identity = 113/423 (26.71%), Postives = 187/423 (44.21%), Query Frame = 1
Query:  226 PKVRLRLQEACDNSDKAKISTVKYCLNPKDSDEDVILKNKEEKII--NYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPE---EIHIRCPSKEAGDNLVKG-RGLGMMNVLQHSVDFR--DSP--PIPRVRDDEATEISKRN---HNGSMSDVMNQKQQKYPVVLREPRCPTAES-----LETWRKS-------------RGDSMSVVMGNKSMFN---DGTHL-MAPK------------------------KRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLM--EKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCP 1311
            P +RLRL EA +N+ + + S V      ++S+   +   K +K I  NY S+ N+N +++ Q+ +I+ P   +     KP     GEESL    R  GS         + + E   +IHIRCP  EA D+L KG +G+G+  +L    + +  D P  PI  +  +EA    +R+     GSM  ++NQ             C T  S     +E+ +KS              G  M+ ++G K  FN   D  +L +AP+                        +  +   G D+E +  +     +  +     +     PR + +G + A +NRGTL N   N + ++P  I    PR+  + +EI ++++G + K L+  +K  Q+      KP   Q +  IA +S+GGA +D L P
Sbjct:   46 PSIRLRLDEAIENAKRNRQSNV---FPSQNSNISNLSWRKLKKPIAGNYDSDCNLNSSEVSQISQILHPNLTKPIIMHKPNVSLRGEESLENSLRGYGSQWFKQITREESLAECQKDIHIRCPRGEALDSLHKGQKGIGVSILLDSKQNDKPIDEPHNPIRSLPSNEAYANWQRDRIKEAGSMEQILNQGWS----------CKTGLSSKLSEMESSQKSCNHSNDDNDRTDHSGVQMAAILGGKLPFNSHSDNNNLSIAPRSIPQSAAANAAREGAGMAERFGLLQHEQFSTGADVEKIEEARAKATLSLDMQHCGNG----PRAKFEGIEYANRNRGTLNNS--NLHSMKPELIPKQTPRIRPEAQEIYESNEGQMCKLLLTNDKLRQH---IPWKPNFYQNTNDIAGKSRGGAAKDCLNP 446          
BLAST of SMED30030030 vs. TrEMBL
Match: R7V103 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_194819 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.247e-9
Identity = 80/299 (26.76%), Postives = 132/299 (44.15%), Query Frame = 1
Query:  406 MDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRI---PEEIHIRCPSKEAGDNLVKGRGLGMMNVLQ--HSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLR-EPRCPTAESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQG 1284
            MD  M       Y + +  PR   +ES     ++RG +  V + E  R    P +  I  PS E  +N  +GR   M  V+    ++ +  + P  R++  E  +  +R+   +M+ V++ ++ +  V  R  PR P+AE+ +   +S G SMS ++   +     T   AP  RVK E   + +    + MS +++  +      P    RV  + +D  EK RG L+    NY  + P S  N  PRV  +G   A   +      L  KYGQ P   +P  RV    ++ A + +G
Sbjct:   57 MDCFMSTSKNADYASPRNPPRVRNDESKSIYAKSRGQMGAVLNAEANRFYYTPRQPKI--PSGEGKENYDRGRHGTMKLVMNIDKNMSYSTARPQLRIKSAEGAQNMQRSRGSNMATVLDGEKNRSYVTPRPAPRAPSAEAQDIAARSNGCSMSQLLDVDANMKYDTPRPAP--RVKSEASENADKDKGNMMSLIIEGKAMRD---PMPVKRVWGEAEDIYEKGRGNLSQMQANYGTM-PLSARN-VPRVKGEGEGNATIGQKGTLCNLFSKYGQMPPSPRPASRVKSEGKRWANKGKG 346          

HSP 2 Score: 68.5514 bits (166), Expect = 8.603e-9
Identity = 69/275 (25.09%), Postives = 119/275 (43.27%), Query Frame = 1
Query:  589 AGDNLVKGRG-LGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPTAESLETWRKSRGDSMSVVM---------------------GNKSMFNDGTHLMA---------------PKKRVKDENGYDIEPLNSSC-MSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEP--RVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAM 1293
            A +NL K RG +        + D+      PRVR+DE+  I  ++  G M  V+N +  ++    R+P+ P+ E  E + + R  +M +VM                     G ++M       MA               P  R       DI   ++ C MSQ++D ++   Y +P+  PRV+ +  +NA+K++G + + I     I+  ++ +P P  RV  +  +I +  +G L+ Q+   YG  P  A+  PRV    +  A   Q G +
Sbjct:   46 AAENLRKSRGTMDCFMSTSKNADYASPRNPPRVRNDESKSIYAKSR-GQMGAVLNAEANRFYYTPRQPKIPSGEGKENYDRGRHGTMKLVMNIDKNMSYSTARPQLRIKSAEGAQNMQRSRGSNMATVLDGEKNRSYVTPRPAPRAPSAEAQDIAARSNGCSMSQLLDVDANMKYDTPRPAPRVKSEASENADKDKGNMMSLI-----IEGKAMRDPMPVKRVWGEAEDIYEKGRGNLS-QMQANYGTMPLSARNVPRVKGEGEGNATIGQKGTL 313          
BLAST of SMED30030030 vs. TrEMBL
Match: A0A2T7NBC6 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_21014 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.825e-8
Identity = 63/221 (28.51%), Postives = 110/221 (49.77%), Query Frame = 1
Query:  676 PRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREP--RCPTAESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNP---------EPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDAL 1305
            PRV+  EA E + RN     +++ + +  + P+V   P  RCP+ E+ + +++SRG    ++ G+       +   AP  RV+ E     E      MS+++ S+S +S  + +  PRV+++  D AE  RG    ++          +HNP         +PRV  +  EIA    G    ++M KYG+ P+ ++P PRV Q + ++A   +GG M++ L
Sbjct:  100 PRVKP-EAEEYASRNLG--TTNIFSSEHYE-PIVTPSPSPRCPSYEARDNYQRSRGQVAGLLGGSGPAVPLES---APAPRVRPEAQSIAENHLGRGMSEILASSSQASEYA-QPVPRVKVEAADQAELARGQRMGKL----------LHNPDGLPATARCQPRVKPEAAEIAHLGAGPQMNKIMNKYGETPRSSRPVPRVKQEAAEMASMDRGGRMQNLL 302          
BLAST of SMED30030030 vs. Planmine SMEST
Match: SMESG000007276.1 (SMESG000007276.1)

HSP 1 Score: 819.305 bits (2115), Expect = 0.000e+0
Identity = 405/407 (99.51%), Postives = 406/407 (99.75%), Query Frame = 1
Query:  136 MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISTVKYCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPTAESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKRRVRSAYR 1356
            MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISTVKY LNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPT+ESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDSNSASSYSSPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKRRVRSAYR
Sbjct:    1 MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISTVKYSLNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPTSESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDSNSASSYSSPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKRRVRSAYR 407          
The following BLAST results are available for this feature:
BLAST of SMED30030030 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8GGD45.067e-2229.53Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267H1G31.542e-2129.53Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A4Z2DCD82.611e-1426.71Uncharacterized protein OS=Schistosoma japonicum O... [more]
R7V1032.247e-926.76Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A2T7NBC61.825e-828.51Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of SMED30030030 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30030030 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000007276.10.000e+099.51SMESG000007276.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030030 ID=SMED30030030|Name=SMED30030030|organism=Schmidtea mediterranea sexual|type=transcript|length=1476bp
TAGAAACATGATTTACTTGATATTTATTCAACATTATTTGTCATTTGATT
TATATTCAATCCATTATTATAAATTAAATTTTGAATCATAATTAATAAAT
TAATTTTCATTGTTTAGTATATATTAAATTAAAATATGTCTACTCTTGCT
GCAATTAAAAATGATGGCAACATAAGAAATAATTCACAATTTCAAGACAA
AAATAAGCCTTTAAAACTTCATACTCCAAAAGTTAGATTGCGTTTACAAG
AGGCTTGTGACAATTCAGATAAAGCGAAAATATCAACTGTCAAATATTGT
TTAAATCCAAAGGATTCTGACGAAGATGTTATTTTAAAGAATAAAGAAGA
GAAAATAATAAATTATTTATCAGAACCTAACATTAATCGTAATAAAATCT
GTCAAATGGATAAAATAATGTTTCCGATAGCTGGTGAAAAATACCAAACT
GACAAACCAGTTCCTCGCTGTATTGGGGAAGAAAGTTTAGGCTATTTAAC
AAGAAATCGTGGATCATTGCTAATAGTAGGAAGCGGTGAGGGTAAGAGAA
TTCCTGAAGAAATTCATATCCGTTGTCCATCGAAAGAAGCCGGAGACAAC
CTTGTAAAAGGTAGGGGCCTTGGAATGATGAATGTTTTACAGCATTCTGT
GGATTTCAGAGACAGTCCGCCAATTCCAAGAGTCAGAGATGACGAAGCAA
CGGAAATCTCGAAGAGAAATCATAATGGTTCAATGTCGGATGTTATGAAC
CAAAAACAACAAAAATACCCTGTTGTGTTACGAGAACCTCGCTGTCCAAC
TGCGGAATCTTTAGAAACCTGGAGAAAAAGTCGTGGGGATTCAATGTCAG
TAGTCATGGGTAACAAGTCGATGTTTAACGACGGTACTCATCTGATGGCA
CCGAAAAAGCGAGTAAAAGATGAGAATGGCTATGACATTGAACCCCTTAA
TAGCTCTTGTATGTCGCAAGTGATGGATTCCAACAGTGCTTCTTCTTATT
CTTCTCCTAAATATGAACCAAGAGTTCGTTTAGATGGCAAAGATAATGCT
GAAAAAAATCGCGGTACACTTACTAATGAAATCTATAATTACAATAAAAT
CCAGCCCGGATCAATACATAATCCGGAACCTCGAGTTTTGCGCCAGGGCA
GAGAAATCGCGGATGCCAGTAAAGGGTTTCTTACCAAACAGTTAATGGAA
AAATATGGTCAGTATCCCAAAGACGCCAAGCCGAAACCACGGGTTACTCA
ATATTCTCAAAAAATTGCTCGACAATCACAAGGGGGTGCAATGCGAGATG
CCTTATGTCCCCCTCGTCAGGCACATTCTAAACGGCGTGTTCGAAGTGCT
TATCGATAATCATTTTGAATTGTGTTCCATTAGATTGATTTTGAAATTTA
TAATTATTTCACATGTAATAATTAAACATTTATTGTAATTCAAGAAATTT
TGATTACTATTGAATAAGCTATTGTG
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protein sequence of SMED30030030-orf-1

>SMED30030030-orf-1 ID=SMED30030030-orf-1|Name=SMED30030030-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=408bp
MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKIS
TVKYCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAG
EKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSK
EAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSM
SDVMNQKQQKYPVVLREPRCPTAESLETWRKSRGDSMSVVMGNKSMFNDG
THLMAPKKRVKDENGYDIEPLNSSCMSQVMDSNSASSYSSPKYEPRVRLD
GKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLT
KQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKR
RVRSAYR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002089reproductive organ
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..407