SMED30030030
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30030030 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of SMED30030030 vs. TrEMBL
Match: A0A1I8GGD4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032595g1 PE=4 SV=1) HSP 1 Score: 109.383 bits (272), Expect = 5.067e-22 Identity = 114/386 (29.53%), Postives = 182/386 (47.15%), Query Frame = 1 Query: 199 KNKPLKLHTPKVRLRLQEAC--DNSDKAKISTVK--YCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKI-CQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRN-RGSLLIVGSGEGKRIPEEI-HIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVR---DDEATEISKRNHNGSMSDVMNQKQQKYPVVLREP----RCPTAESLE-TWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCP 1311 ++KP+K+H+PKVR +A N ++++ S VK + P++++ + + I +E I R + +MDK++ P YQ + P+C E+S + RN RG+ + +E +RCPS A +NL KGRG M L H + +PP P +R E+ E R+ G MS V NQ + ++P R E+ E ++RG + + + D P RV+ E + + M + P+ PRVRL G + A K +GTLT+E YNY ++ P P+V + +E A+ + +TK L++KYGQ P D PKP V + A +S+G AM+D + P Sbjct: 59 RSKPIKVHSPKVRTGWDKAAVVANGERSRSSAVKANFDYRPENAERTEKQQPRGSAIDGRFAE--IQRKQTHTEMDKVLNPSKERFYQPEM-NPKCPTEDSKEFYRRNQRGTYGFMEPPRANYPTDEPGKVRCPSAAARENLEKGRG--MAGRLIHQEERSRTPP-PSLRLGASQESRENYDRSRGGCMSAVFNQSENIR-AGAQDPAPAGRVIKGEAAEQNASRNRGQTAQLFAPGRGAQPD----PQPAPRVRPE-AQETAAREGAGMRGTF---YGGPQTEPQPAPRVRLGGGEVASKLQGTLTSEQYNYRQMP--PDPPPAPKVRPEAQEYANRERAVVTKNLLQKYGQLPSDLPPKPHVPPQAWNTAYRSRGSAMKDCINP 427
BLAST of SMED30030030 vs. TrEMBL
Match: A0A267H1G3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009461g1 PE=4 SV=1) HSP 1 Score: 107.842 bits (268), Expect = 1.542e-21 Identity = 114/386 (29.53%), Postives = 181/386 (46.89%), Query Frame = 1 Query: 199 KNKPLKLHTPKVRLRLQEAC--DNSDKAKISTVK--YCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKI-CQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRN-RGSLLIVGSGEGKRIPEEI-HIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVR---DDEATEISKRNHNGSMSDVMNQKQQKYPVVLREP----RCPTAESLE-TWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCP 1311 ++KP+K+H+PKVR +A N ++++ S VK + P++++ + + I +E I R + +MDK++ P YQ + P+C E+S + RN RG+ + +E +RCPS A +NL KGRG M L H + +PP P +R E E R+ G MS V NQ + ++P R E+ E ++RG + + + D P RV+ E + + M + P+ PRVRL G + A K +GTLT+E YNY ++ P P+V + +E A+ + +TK L++KYGQ P D PKP V + A +S+G AM+D + P Sbjct: 59 RSKPIKVHSPKVRTGWDKAAVVANGERSRSSAVKANFDYRPENAERTEKQQPRGSAIDGRFAE--IQRKQTHTEMDKVLNPSKERFYQPEM-NPKCPTEDSKEFCRRNQRGTYGFMEPLRANYPTDEPGKVRCPSAAARENLEKGRG--MAGRLIHQEERSRTPP-PSLRLGASQETRENYDRSRGGCMSAVFNQSENIR-AGAQDPAPAGRVIKGEAAEQNASRNRGQTAQLFAPGRGAQPD----PQPAPRVRPE-AQETAAREGAGMRGTF---YGGPQTEPQPAPRVRLGGGEVASKLQGTLTSEQYNYRQMP--PDPPPAPKVRPEAQEYANRERAVVTKNLLQKYGQLPSDLPPKPHVPPQAWNTAYRSRGSAMKDCINP 427
BLAST of SMED30030030 vs. TrEMBL
Match: A0A4Z2DCD8 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002403 PE=4 SV=1) HSP 1 Score: 86.2705 bits (212), Expect = 2.611e-14 Identity = 113/423 (26.71%), Postives = 187/423 (44.21%), Query Frame = 1 Query: 226 PKVRLRLQEACDNSDKAKISTVKYCLNPKDSDEDVILKNKEEKII--NYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPE---EIHIRCPSKEAGDNLVKG-RGLGMMNVLQHSVDFR--DSP--PIPRVRDDEATEISKRN---HNGSMSDVMNQKQQKYPVVLREPRCPTAES-----LETWRKS-------------RGDSMSVVMGNKSMFN---DGTHL-MAPK------------------------KRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLM--EKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCP 1311 P +RLRL EA +N+ + + S V ++S+ + K +K I NY S+ N+N +++ Q+ +I+ P + KP GEESL R GS + + E +IHIRCP EA D+L KG +G+G+ +L + + D P PI + +EA +R+ GSM ++NQ C T S +E+ +KS G M+ ++G K FN D +L +AP+ + + G D+E + + + + + PR + +G + A +NRGTL N N + ++P I PR+ + +EI ++++G + K L+ +K Q+ KP Q + IA +S+GGA +D L P Sbjct: 46 PSIRLRLDEAIENAKRNRQSNV---FPSQNSNISNLSWRKLKKPIAGNYDSDCNLNSSEVSQISQILHPNLTKPIIMHKPNVSLRGEESLENSLRGYGSQWFKQITREESLAECQKDIHIRCPRGEALDSLHKGQKGIGVSILLDSKQNDKPIDEPHNPIRSLPSNEAYANWQRDRIKEAGSMEQILNQGWS----------CKTGLSSKLSEMESSQKSCNHSNDDNDRTDHSGVQMAAILGGKLPFNSHSDNNNLSIAPRSIPQSAAANAAREGAGMAERFGLLQHEQFSTGADVEKIEEARAKATLSLDMQHCGNG----PRAKFEGIEYANRNRGTLNNS--NLHSMKPELIPKQTPRIRPEAQEIYESNEGQMCKLLLTNDKLRQH---IPWKPNFYQNTNDIAGKSRGGAAKDCLNP 446
BLAST of SMED30030030 vs. TrEMBL
Match: R7V103 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_194819 PE=4 SV=1) HSP 1 Score: 70.4774 bits (171), Expect = 2.247e-9 Identity = 80/299 (26.76%), Postives = 132/299 (44.15%), Query Frame = 1 Query: 406 MDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRI---PEEIHIRCPSKEAGDNLVKGRGLGMMNVLQ--HSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLR-EPRCPTAESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQG 1284 MD M Y + + PR +ES ++RG + V + E R P + I PS E +N +GR M V+ ++ + + P R++ E + +R+ +M+ V++ ++ + V R PR P+AE+ + +S G SMS ++ + T AP RVK E + + + MS +++ + P RV + +D EK RG L+ NY + P S N PRV +G A + L KYGQ P +P RV ++ A + +G Sbjct: 57 MDCFMSTSKNADYASPRNPPRVRNDESKSIYAKSRGQMGAVLNAEANRFYYTPRQPKI--PSGEGKENYDRGRHGTMKLVMNIDKNMSYSTARPQLRIKSAEGAQNMQRSRGSNMATVLDGEKNRSYVTPRPAPRAPSAEAQDIAARSNGCSMSQLLDVDANMKYDTPRPAP--RVKSEASENADKDKGNMMSLIIEGKAMRD---PMPVKRVWGEAEDIYEKGRGNLSQMQANYGTM-PLSARN-VPRVKGEGEGNATIGQKGTLCNLFSKYGQMPPSPRPASRVKSEGKRWANKGKG 346 HSP 2 Score: 68.5514 bits (166), Expect = 8.603e-9 Identity = 69/275 (25.09%), Postives = 119/275 (43.27%), Query Frame = 1 Query: 589 AGDNLVKGRG-LGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPTAESLETWRKSRGDSMSVVM---------------------GNKSMFNDGTHLMA---------------PKKRVKDENGYDIEPLNSSC-MSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEP--RVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAM 1293 A +NL K RG + + D+ PRVR+DE+ I ++ G M V+N + ++ R+P+ P+ E E + + R +M +VM G ++M MA P R DI ++ C MSQ++D ++ Y +P+ PRV+ + +NA+K++G + + I I+ ++ +P P RV + +I + +G L+ Q+ YG P A+ PRV + A Q G + Sbjct: 46 AAENLRKSRGTMDCFMSTSKNADYASPRNPPRVRNDESKSIYAKSR-GQMGAVLNAEANRFYYTPRQPKIPSGEGKENYDRGRHGTMKLVMNIDKNMSYSTARPQLRIKSAEGAQNMQRSRGSNMATVLDGEKNRSYVTPRPAPRAPSAEAQDIAARSNGCSMSQLLDVDANMKYDTPRPAPRVKSEASENADKDKGNMMSLI-----IEGKAMRDPMPVKRVWGEAEDIYEKGRGNLS-QMQANYGTMPLSARNVPRVKGEGEGNATIGQKGTL 313
BLAST of SMED30030030 vs. TrEMBL
Match: A0A2T7NBC6 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_21014 PE=4 SV=1) HSP 1 Score: 67.781 bits (164), Expect = 1.825e-8 Identity = 63/221 (28.51%), Postives = 110/221 (49.77%), Query Frame = 1 Query: 676 PRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREP--RCPTAESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNP---------EPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDAL 1305 PRV+ EA E + RN +++ + + + P+V P RCP+ E+ + +++SRG ++ G+ + AP RV+ E E MS+++ S+S +S + + PRV+++ D AE RG ++ +HNP +PRV + EIA G ++M KYG+ P+ ++P PRV Q + ++A +GG M++ L Sbjct: 100 PRVKP-EAEEYASRNLG--TTNIFSSEHYE-PIVTPSPSPRCPSYEARDNYQRSRGQVAGLLGGSGPAVPLES---APAPRVRPEAQSIAENHLGRGMSEILASSSQASEYA-QPVPRVKVEAADQAELARGQRMGKL----------LHNPDGLPATARCQPRVKPEAAEIAHLGAGPQMNKIMNKYGETPRSSRPVPRVKQEAAEMASMDRGGRMQNLL 302
BLAST of SMED30030030 vs. Planmine SMEST
Match: SMESG000007276.1 (SMESG000007276.1) HSP 1 Score: 819.305 bits (2115), Expect = 0.000e+0 Identity = 405/407 (99.51%), Postives = 406/407 (99.75%), Query Frame = 1 Query: 136 MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISTVKYCLNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPTAESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDXXXXXXXXXPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKRRVRSAYR 1356 MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISTVKY LNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPT+ESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDSNSASSYSSPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKRRVRSAYR Sbjct: 1 MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISTVKYSLNPKDSDEDVILKNKEEKIINYLSEPNINRNKICQMDKIMFPIAGEKYQTDKPVPRCIGEESLGYLTRNRGSLLIVGSGEGKRIPEEIHIRCPSKEAGDNLVKGRGLGMMNVLQHSVDFRDSPPIPRVRDDEATEISKRNHNGSMSDVMNQKQQKYPVVLREPRCPTSESLETWRKSRGDSMSVVMGNKSMFNDGTHLMAPKKRVKDENGYDIEPLNSSCMSQVMDSNSASSYSSPKYEPRVRLDGKDNAEKNRGTLTNEIYNYNKIQPGSIHNPEPRVLRQGREIADASKGFLTKQLMEKYGQYPKDAKPKPRVTQYSQKIARQSQGGAMRDALCPPRQAHSKRRVRSAYR 407 The following BLAST results are available for this feature:
BLAST of SMED30030030 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30030030 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30030030 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30030030 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30030030 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30030030 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030030 ID=SMED30030030|Name=SMED30030030|organism=Schmidtea mediterranea sexual|type=transcript|length=1476bpback to top protein sequence of SMED30030030-orf-1 >SMED30030030-orf-1 ID=SMED30030030-orf-1|Name=SMED30030030-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=408bp MSTLAAIKNDGNIRNNSQFQDKNKPLKLHTPKVRLRLQEACDNSDKAKISback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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