Rootletin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Expression Details Rootletin ( H.2.8b, crocc ) is expressed during Stage 5, Stage 6, Stage 7 and Stage 8 in the Category 5 cell, proximal tubule, integumental system, protonephridia, renal system and epidermis
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Anatomical Expression
PAGE et. al., 2020SMED30030007 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 40Note: Hover over icons to view figure legend
Homology
BLAST of Rootletin vs. Ensembl Human
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:HGNC Symbol;Acc:HGNC:21299]) HSP 1 Score: 895.19 bits (2312), Expect = 0.000e+0 Identity = 706/1937 (36.45%), Postives = 1145/1937 (59.11%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKM--QFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXX-AKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKS---------------------GLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINS-RHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
+GL++ ++ELR++L+EE YRRKL +Q+ QQ+QAQLVQRLQ K+LQYK++C+ELE ++ + G+ E ++ + + + DLES LI+LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W R R+ELE RE WR EE SFN Y ++EHS L L R + FRR E+K TER++ L E R +R +Q A LS ++ AES+ + + E+++ + LE QLR+K ++L Q + + + +L RV EL + +L+ QNLEKD+ L AL+ ++ ++++A + ED E + Q LR+++Q VL+DS+ DA + + PRR+ S +P +D+ T A + SA++K+ LQ+Q+++ + +S++ ++Q+ D+E+ERR LE Q+ +RD+ + + +D+ RE R ++A + S E+S L Q E+ E E+L++A EL+R R+ LE+EQED +D R +E ER R ++ LE K S +++V +EA+++ L++++L EK E+ +AL++ E + ELE+ + K + E +L++++SKLS+ E L + + +R + QLE E+S+L +R EQE + GL+ L +EQ L + R E+ L EQ+ L RQ S +EL Q RRE +R ++ ++EKE L KE L VQ+ A ERE R SE L ++E LE +L++ Q+ + QLE R+EQLE E Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL + E+ E ER +L + E+++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H EV+ L +DL +D L+E +L T L++ E+ RD LRRE++E RKL+E++E +E ++E EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E +E ELR L++VE++RL+ +RELQELRR +K LD+E +L +E+ +LQ R+A E E E+RRE GL+Q++++ EAS E +R++L AQR + + E ++++R +E +R ++ D L+ + A+ EL + LS AEGR LE+ +ARVE ++ E +L + S+LRR +G + R S + G+G P T S + S P+ +P D++PEAVR ALREF+Q+L A+R+RD+ Q +L +L E E + + A +R +QLQ+A+ ++EE ++ +DGRLS Q L +QEE++RR+ER+R+A ++++ +R+ + ESE R +++++K + ++ +LE + ++ K+ ++ +E+R +L+L +RSLEG+LQR ++ L+D+E + + L DRV+SL +Q+ D E K+ +LQLT++R++ ALAK EE +DK++ L +L+ S L ++K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ ++ D E +R E EG +Q ++ QRQE E QKL E+ LQER+ S+QR LA EAEK+E+ ++ +LEKDR AL+++L KVER+KL++ E + E+ LD+ L E E E QRQ+ L+ Q+ EQ+H +E+++ + + E+ E+E LR++ Q ERTLE RERAHRQRV+GLEEQ+STLK QL E++R
Sbjct: 135 RGLVRQSVELRRQLQEEQASYRRKLQAYQEGQQRQAQLVQRLQGKILQYKKRCSELEQQLLERSGELEQQRLR--------------DTEHSQDLESALIRLEEEQQRSASLAQVNAMLREQLDQAGSANQALSEDIRKVTNDWTRCRKELEHREAAWRREEESFNAYFSNEHSRLLLLWRQVVGFRRLVSEVKMFTERDLLQLGGELARTSRAVQEAGLGLSTGLRLAESRAEAALEKQALLQAQLEEQLRDKVLREKDLAQQQMQSDLDKADLSARVTELGLAVKRLEKQNLEKDQVNKDLTEKLEALESLR---------LQEQAA-LETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGSLRGLSGQRTPSPPRRSSPGRGRSPRRGPSPACSDSSTLALIHSALHKRQLQVQDMRGRYEASQDLLGTLRKQLSDSESERRALEEQLQRLRDKTDGAMQAHEDAQREVQRLRSANELLSREKSNLAHSLQVAQQQAEELRQEREKLQAAQEELRRQRDRLEEEQEDAVQDGARVRRELERSHRQLEQLEGKRSVLAKELVEVREALSRATLQRDMLQAEKAEVAEALTKAEAGRVELELSMTKLRAEEASLQDSLSKLSALNESLAQDKLDLNRLVAQLEEEKSALQGRQRQAEQEATVAREEQERLEELRLEQEVARQGLEGSLRVAEQAQEALEQQLPTLRH---------------------ERSQLQEQLAQLSRQLSGREQELEQARREAQRQVEALERAAREKEALAKEHAGLAVQLVAAEREGRTLSEEATRLRLEKEALEGSLFEVQRQLAQLEARREQLEAEGQALLLAKETLTGELAGLRQQIIATQEKASLDKELMAQKLVQAEREAQASLREQRAAHEEDLQRLQREKEAAWRELEAERAQLQSQLQREQEELLARLEAEKEELSEEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLATISLEMERQKRDAQSRQEQDRSTVNALTSELRDLRAQREEAAAAHAQEVRRLQEQARDLGKQRDSCLREAEELRTQLRLLEDARDGLRRELLEAQRKLRESQEGREVQRQEAGELRRSLGEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARTAVGKEAGELRTGLQEVERSRLEARRELQELRRQMKMLDSENTRLGRELAELQGRLALGERAEKESRRETLGLRQRLLKGEASLEVMRQELQVAQRKLQEQEGEFRTRERRLLGSLEEARGTEKQQLDHARGLELKLEAARAEAAELGLRLSAAEGRAQGLEAELARVEVQRRAAEAQLGGLRSALRRGLGLGRAPSPAPRPVPGSPA----------------------RDAPAEGSGEGLNSPSTLECSPGSQPPSPGPATSPASP-DLDPEAVRGALREFLQELRSAQRERDELRTQTSALNRQLAEMEAERDSATSRARQLQKAVAESEEARRSVDGRLSGVQAELALQEESVRRSERERRATLDQVATLERSLQATESELRASQEKISKMKANETKLEGDKRRLKEVLDASESRTVKLELQRRSLEGELQRSRLGLSDREAQAQALQDRVDSLQRQVADSEVKAGTLQLTVERLNGALAKVEESEGALRDKVRGLTEALAQSSASLNSTRDKNLHLQKALTACEHDRQVLQERLDAARQALSEARKQSSSLGEQVQTLRGEVADLELQRVEAEGQLQQLREVLRQRQEGEAAALNTVQKLQDERRLLQERLGSLQRALAQLEAEKREVERSALRLEKDRVALRRTLDKVEREKLRSHEDTVRLSAEKGRLDRTLTGAELELAEAQRQIQQLEAQVVVLEQSHSPAQLEVDAQQQQLELQQEVERLRSAQAQTERTLEARERAHRQRVRGLEEQVSTLKGQLQQELRR 2003
BLAST of Rootletin vs. Ensembl Human
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:HGNC Symbol;Acc:HGNC:21299]) HSP 1 Score: 567.385 bits (1461), Expect = 1.478e-172 Identity = 450/1264 (35.60%), Postives = 731/1264 (57.83%), Query Frame = 3
Query: 438 DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXX-AKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKS---------------------GLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGF 4100
DLES LI+LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W R R+ELE RE WR EE SFN Y ++EHS L L R + FRR E+K TER++ L E R +R +Q A LS ++ AES+ + + E+++ + LE QLR+K ++L Q + + + +L RV EL + +L+ QNLEKD+ L AL+ ++ ++++A + ED E + Q LR+++Q VL+DS+ DA + + PRR+ S +P +D+ T A + SA++K+ LQ+Q+++ + +S++ ++Q+ D+E+ERR LE Q+ +RD+ + + +D+ RE R ++A + S E+S L Q E+ E E+L++A EL+R R+ LE+EQED +D R +E ER R ++ LE K S +++V +EA+++ L++++L EK E+ +AL++ E + ELE+ + K + E +L++++SKLS+ E L + + +R + QLE E+S+L +R EQE + GL+ L +EQ ++A+++Q L L E+ L EQ+ L RQ S +EL Q RRE +R ++ ++EKE L KE L VQ+ A ERE R SE L ++E LE +L++ Q+ + QLE R+EQLE E Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL + E+ E ER +L + E+++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H EV+ L +DL +D L+E +L T L++ E+ RD LRRE++E RKL+E++E +E ++E EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E +E ELR L++VE++RL+ +RELQELRR +K LD+E +L +E+ +LQ R+A E E E+RRE GL+Q++++ EAS E +R++L AQR + + E ++++R +E +R ++ D L+ + A+ EL + LS AEGR LE+ +ARVE ++ E +L + S+LRR +G
Sbjct: 74 DLESALIRLEEEQQRSASLAQVNAMLREQLDQAGSANQALSEDIRKVTNDWTRCRKELEHREAAWRREEESFNAYFSNEHSRLLLLWRQVVGFRRLVSEVKMFTERDLLQLGGELARTSRAVQEAGLGLSTGLRLAESRAEAALEKQALLQAQLEEQLRDKVLREKDLAQQQMQSDLDKADLSARVTELGLAVKRLEKQNLEKDQVNKDLTEKLEALESLR---------LQEQAA-LETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGSLRGLSGQRTPSPPRRSSPGRGRSPRRGPSPACSDSSTLALIHSALHKRQLQVQDMRGRYEASQDLLGTLRKQLSDSESERRALEEQLQRLRDKTDGAMQAHEDAQREVQRLRSANELLSREKSNLAHSLQVAQQQAEELRQEREKLQAAQEELRRQRDRLEEEQEDAVQDGARVRRELERSHRQLEQLEGKRSVLAKELVEVREALSRATLQRDMLQAEKAEVAEALTKAEAGRVELELSMTKLRAEEASLQDSLSKLSALNESLAQDKLDLNRLVAQLEEEKSALQGRQRQAEQEATVAREEQERLEELRLEQEVARQGLEGSLRVAEQ------AQEALEQQ---------------LPTLRHERSQLQEQLAQLSRQLSGREQELEQARREAQRQVEALERAAREKEALAKEHAGLAVQLVAAEREGRTLSEEATRLRLEKEALEGSLFEVQRQLAQLEARREQLEAEGQALLLAKETLTGELAGLRQQIIATQEKASLDKELMAQKLVQAEREAQASLREQRAAHEEDLQRLQREKEAAWRELEAERAQLQSQLQREQEELLARLEAEKEELSEEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLATISLEMERQKRDAQSRQEQDRSTVNALTSELRDLRAQREEAAAAHAQEVRRLQEQARDLGKQRDSCLREAEELRTQLRLLEDARDGLRRELLEAQRKLRESQEGREVQRQEAGELRRSLGEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARTAVGKEAGELRTGLQEVERSRLEARRELQELRRQMKMLDSENTRLGRELAELQGRLALGERAEKESRRETLGLRQRLLKGEASLEVMRQELQVAQRKLQEQEGEFRTRERRLLGSLEEARGTEKQQLDHARGLELKLEAARAEAAELGLRLSAAEGRAQGLEAELARVEVQRRAAEAQLGGLRSALRRGLGL 1306
BLAST of Rootletin vs. Ensembl Human
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:HGNC Symbol;Acc:HGNC:21299]) HSP 1 Score: 301.982 bits (772), Expect = 2.065e-85 Identity = 235/671 (35.02%), Postives = 390/671 (58.12%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKM--QFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVX-XXXXXXXXXXXXXXXXXXXXAKTPPFAD-ATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKS 2105
+GL++ ++ELR++L+EE YRRKL +Q+ QQ+QAQLVQRLQ K+LQYK++C+ELE ++ + G+ E ++ + + + DLES LI+LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W R R+ELE RE WR EE SFN Y ++EHS L L R + FRR E+K TER++ L E R +R +Q A LS ++ AES+ + + E+++ + LE QLR+K ++L Q + + + +L RV EL + +L+ QNLEKD+ L AL+ ++ + A + ED E + Q LR+++Q VL+DS+ DA + + + SP R + + R +P +D +T A + SA++K+ LQ+Q+++ + +S++ ++Q+ D+E+ERR LE Q+ +RD+ + + +D+ RE R ++A + S E+S L Q E+ E E+L++A EL+R R+ LE+EQED +D R +E ER R ++ LE K S +++V +EA+++ L++++L EK E+ +AL++ E + ELE+ + K + E +L++++SKLS+ E L + + +R + QLE E+S+L +R EQE +
Sbjct: 29 RGLVRQSVELRRQLQEEQASYRRKLQAYQEGQQRQAQLVQRLQGKILQYKKRCSELEQQLLERSGELEQQRLR--------------DTEHSQDLESALIRLEEEQQRSASLAQVNAMLREQLDQAGSANQALSEDIRKVTNDWTRCRKELEHREAAWRREEESFNAYFSNEHSRLLLLWRQVVGFRRLVSEVKMFTERDLLQLGGELARTSRAVQEAGLGLSTGLRLAESRAEAALEKQALLQAQLEEQLRDKVLREKDLAQQQMQSDLDKADLSARVTELGLAVKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAA----------LETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGSLRGLSGQRTPSPPRRSSPGRGRSPRRGPSPACSDSSTLALIHSALHKRQLQVQDMRGRYEASQDLLGTLRKQLSDSESERRALEEQLQRLRDKTDGAMQAHEDAQREVQRLRSANELLSREKSNLAHSLQVAQQQAEELRQEREKLQAAQEELRRQRDRLEEEQEDAVQDGARVRRELERSHRQLEQLEGKRSVLAKELVEVREALSRATLQRDMLQAEKAEVAEALTKAEAGRVELELSMTKLRAEEASLQDSLSKLSALNESLAQDKLDLNRLVAQLEEEKSALQGRQRQAEQEAT 675 HSP 2 Score: 69.707 bits (169), Expect = 5.751e-11 Identity = 43/98 (43.88%), Postives = 73/98 (74.49%), Query Frame = 3
Query: 2790 ENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDK 3083
E ER +L + E+++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+
Sbjct: 740 EAERAQLQSQLQREQEELLARLEAEKEELSEEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLATISLEMERQKRDAQSRQEQDR 837
BLAST of Rootletin vs. Ensembl Human
Match: CROCC2 (ciliary rootlet coiled-coil, rootletin family member 2 [Source:HGNC Symbol;Acc:HGNC:51677]) HSP 1 Score: 265.388 bits (677), Expect = 1.311e-70 Identity = 331/1177 (28.12%), Postives = 613/1177 (52.08%), Query Frame = 3
Query: 1488 EIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEEL-NDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQE-ALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERD-DMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQ--SLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRH-------HDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKE-------TETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRM 4961
E E+LR + E + + + + E++ LE + L + + E + L++ ELQR + +E+ ER + E Q + LE+K+S +E++ + +EA++ QL+++V+ E++ + AL+R E S A+LE+ V + ++ V ++++ +++ +E L DK A NH + QLE ER L +++ LEQE++ ++L ++EQ+ AL + + +Q Q +LE E+LE + + L REK L EQ+G + QK AL E+L Q ++ E ++ + K+ L++E+ +L+ + A ER+ R +E A L +R+ LE++L +AQQL +L +EQLE E + + ++ L E+ +L+ +++++ K+ +L + Q ++E + A++ + +H E + +L + E+ E+E + R +E++ +++ E++ + EI ++++ERD+SLL E++ QQ +LSL E E+ ++E EL L+R+++E+ ++QEQ + I+ + ELK+ ++QFED H+ E +L ++DL + +E L L +A+E LR+E+ + E++E L RE E +R L +E REK+ ++ N LR I E E+ R + +QK+ + +E +QE + LR +L ++E+A D ++EL+EL R ++ L E + + E ++LQ + +++ L+++ + EA E RK++ QR + ++E ++ + + SR + L +S+ + + L+ L A R+ +LE +A+ EGA+++ E +L + S+LRR +G ++++ + S TKG D++ A SP R P S R S +P +D+ V+ LR+FVQ+L +A+R+RDD+ Q +L SRL E E + A++R+ QLQ+AL +AEE ++ ++G LSSA+ A +Q+E +RR E + A + + EKR+L+++L R H L ++ K ++ + N E+R Q A+ SLE L++M+ +E E E LD +NSL +++ ++Q LQ + M
Sbjct: 461 EREALRGQLEAQRLEVQQCRASCKLLGREKAALEMVVEELKGKADAADAEKQGLEAEAAELQRSLLLQAERREELALRRERSCRALETSQGRLQQLEEKVSGLREELASVREALSTAQLQRDVVESEREGLRSALARAECSNADLELLVRRLKSEGVEQRDSLAAMAALMEGLAQDKSALNHLAL-QLEQERDQLREQRKTLEQERARAGEQLAQAEQQLALERAERRGLQ-QACGRLEQRQEQLEGQAALLGREKAQLQEQVGQVTCQKQALEEQLAQSLQDQEAQMGTLQQALQGKDALSEERAQLLAKQEALERQGRLAAEEAADLRVERDSLESSLLEAQQLATKL---QEQLEEEARSAGLARQALQVEMEQLQSDWEVQEMKLRQDTVRLQRQVAQQEREAQRALESQALAHREALAQLQREKETLSLTLAEEKE--VARCQLEQEKELVTKSAAEREALKGEIQSLKQERDESLLQLEHKMQQVMALSLKETERSLLSE-------ELSRARRTLERVQQEAQSQQEQAQATISATTEELKALQAQFEDAITAHQRETTALRESLQDLAAERGDVEREAERLRAQLTVAQEGLAALRQELQGVE-------ESREGLHREAQEARRALSDEAREKDVLLLFNSELRATICRAEQEKASFKRSKEEKEQKLLILEEAQAALQQEASALRAHLWELEQAGGDARQELRELHRQVRTLKAENQRRSGEAHELQAQCSQEV----------LELRRQAAKAEAKHEGARKEVLGLQRKLAEVEAAGEAHGQRLQEHLRESRGAEQTLRAELHSVTRKLQEASGVADALQARLDQACHRIHSLEQELAQAEGARQDAEAQLGRLCSTLRRGLGLQRQSPWASPEQPGSP------------------TKGSDSSQALPGQQGTSPPAR---PHSPLRWPSPTPGGRSSELMDVA--TVQDILRDFVQKLREAQRERDDSRIQMATLSSRLSEAECRCARAQSRVGQLQKALAEAEEGQRRVEGALSSARAARALQKEALRRLELEHLA----------SVRAAGQEKRRLQEQLETLRQALEESRRHSQGLAKQGKLLEEQLTNLEHRC---QKAEVSLE-PLRQMEQETLKREEDVARLGAEKEQLDQSLNSLHQEVDGALRQNQQLQAQMTEM 1569 HSP 2 Score: 84.3445 bits (207), Expect = 2.599e-15 Identity = 66/209 (31.58%), Postives = 105/209 (50.24%), Query Frame = 3
Query: 432 NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTAL 1058
++DLE L +LE ++++ L + N L+EQL ++N L R+++ ++ QR + ELE R W + + +L L R + EL+ +TER + +L+ + R AR L TAC NL N++ LS S L QLR+K E+ Q R++ E LQ R+ E T+L +KL QN +K + + AL
Sbjct: 147 SVDLEEALGRLEAAEERSTGLCQVNALLREQLEHMKKANDALGRELAGMTGSVQRLQGELELRR--WAQRQTRSGGL--GQPRDLLLLWRQAVVLGTDLAELRVATERGLADLQADTARTARRLHTACLNLDSNLR-------LSA---SSTASTLGQQLRDKAGEMLQLQGRWDAEKVALQARLSEQTLLVEKLTEQNEQKAKTIAAL 341 HSP 3 Score: 62.3882 bits (150), Expect = 1.399e-8 Identity = 84/278 (30.22%), Postives = 143/278 (51.44%), Query Frame = 3
Query: 5010 KMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQ-NNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
+M L+ LS + + Q+++ QLQKAL +E+ +R +E L S++ A K L +E + +++ E RR L Q TL ++A +E + Q L + L+E++ +++ +K E+ LE R ++E++ LK EE ++ E+ +LDQ L L E RQ LQ Q+ E EQAH L ++ ++H+++++ E E L + Q + LE +E H+Q+VK LEEQ+++LK+QL E+Q R
Sbjct: 1390 QMATLSSRLSEAECRCARAQSRVGQLQKALAEAEEGQRRVEGALSSARAARALQKEALRRLELEHLASVRA--AGQEKRR-----LQEQLETL----RQALEESRRHSQGLAKQGKLLEEQLTNLEHR-----CQKAEV-----SLEPLR--------QMEQETLKREEDVARLGAEKEQLDQSLNSLHQEVDGALRQNQQLQAQMTEMEQAHTQRLQDLTAQHQRDLATEAERLHGARPQATQALESQEWTHQQQVKVLEEQVASLKEQLDQEVQWR 1638
BLAST of Rootletin vs. Ensembl Human
Match: CEP250 (centrosomal protein 250 [Source:HGNC Symbol;Acc:HGNC:1859]) HSP 1 Score: 203.756 bits (517), Expect = 1.863e-51 Identity = 269/1011 (26.61%), Postives = 506/1011 (50.05%), Query Frame = 3
Query: 309 YKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNL----------ENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTAL-QRIKNGDTAGEINMEDKAT--RVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELN-DKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIME 3299
Y+ C ELE +++ G P + + E D L L++LEEEQ++ L E N +L+ + +A N+ L D+ KL+ +W R+R+EL ++E W+ E+ F YL EH L +L R + FRR F+E+K++T+R++ LK E R + L T C L+ +S E S + R+ L E + E+++EL +++ + E ELQ RV EL+ L + + QN + ++ + AL + ++ +T ME +A+ R E+ S+ Q +++I+QV++ + D A+ +G E + + S + + AD V+S + ++ +Q+L+ +L +E+ ++Q D E E + A+R ++ L ++D + + ++ S ER L+K R L Q+E E RL+ N ELQ ++ + ++E+ +++L ++E ER Q + LE K S +++ +EA+ LE E+L QE+ E+ AL+R E S AEL N +T +L KLS+ E L DK N + + QLE E S+ + EQ ++ L+ +L +E+ L + ++++ E L+ +L ++ EK ++ +++ + Q+ A +L Q +E +R + +EKE L +EK L V++ A ER+ + +E + L+S +E LE++L++AQQ +E K QLE + Q + KE + GE+ L+ E E + R+ + Q ++E K A++Q++ +H ++V +L + E R + E + + ++ER+ M E +E E+ IQ +R++ AE+ Q E+E+ ++ E L++TQ EL +L+R++++ ++ ++++ L ++L+ + + ++ + H ++ +L L K K+V DL + L ++++ + +E+ E
Sbjct: 66 YRSWCQELEKRLEA---------TGGPIPQRWENVEEPNLDEL-----LVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNG--SGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNI-AQGSGHENSLELDSSIFSQFDYQD------------ADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGK-------ALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLL-QLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALE--SLEREKM--ELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQE 1035
BLAST of Rootletin vs. Ensembl Celegans
Match: lfi-1 (Lin-5 (Five) Interacting protein [Source:UniProtKB/TrEMBL;Acc:H2L0I3]) HSP 1 Score: 91.6633 bits (226), Expect = 7.485e-18 Identity = 113/457 (24.73%), Postives = 230/457 (50.33%), Query Frame = 3
Query: 4479 ENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFR------HHDARL---EEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNM-SLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQL 5819
E R + ++ L DAEE+++ ++ RL SA+T L QEE +++ + +R+ M K+ + E++ R L ++L R H D R +EE S + QL+ R + D + ++ + E+E ++L +++ L ++ + K Q ++ D++ L K E S + + +++ S ++H QL L++++ Q L + ++ LE L + + + + K + + + R+ +Q L D+ ++ +E ++ + E +L Q+ + +EK + + ++R LA E E++ + +K++ L K + +E +K +T+ +T +R +++ L +E EN EL + LQ Q+A+ E + + ++E+ ++ R+E ++ +R QIE+ +E RER HR R+K LE+QI+ L+DQL
Sbjct: 1778 ETRYRNIEDNLQDAEEERRALESRLQSAKTLLRSQEEALKQRDEERRQMKSKMVAAELQARGKEAQLRHLNEQLKNLRTDLDNAHTDIRSLRDKEEQWDSSRF---------QLETKMRESDSDTNKYQLQIASFESERQILTEKIKELDGALRLSDSKVQDMKDDTDKLRRDLTKAE---SVENELRKTIDIQSKTSHEYQL--LKDQLLNTQNELNGANNRKQQLENELLNVRSEVRDYKQRVHDVNNRVSELQRQLQDANTEKNRVEDRFLSVEKVVNTMRTTETDLRQQLETAKNEKRVATKELEDLKRRLAQLENERRNSSQLSDGWKKEKITLLKKIELLENEKRRTDAAIRETALQREAIEKSLNAMERENKELYKNCAQLQQQIAQLEMENGNRILELTNKQREEQERQLIRMRQEKGQIEKVIENRERTHRNRIKQLEDQIAILRDQL 2220
BLAST of Rootletin vs. Ensembl Celegans
Match: lfi-1 (Lin-5 (Five) Interacting protein [Source:UniProtKB/TrEMBL;Acc:H2L0I3]) HSP 1 Score: 91.6633 bits (226), Expect = 7.485e-18 Identity = 113/457 (24.73%), Postives = 230/457 (50.33%), Query Frame = 3
Query: 4479 ENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFR------HHDARL---EEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNM-SLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQL 5819
E R + ++ L DAEE+++ ++ RL SA+T L QEE +++ + +R+ M K+ + E++ R L ++L R H D R +EE S + QL+ R + D + ++ + E+E ++L +++ L ++ + K Q ++ D++ L K E S + + +++ S ++H QL L++++ Q L + ++ LE L + + + + K + + + R+ +Q L D+ ++ +E ++ + E +L Q+ + +EK + + ++R LA E E++ + +K++ L K + +E +K +T+ +T +R +++ L +E EN EL + LQ Q+A+ E + + ++E+ ++ R+E ++ +R QIE+ +E RER HR R+K LE+QI+ L+DQL
Sbjct: 1778 ETRYRNIEDNLQDAEEERRALESRLQSAKTLLRSQEEALKQRDEERRQMKSKMVAAELQARGKEAQLRHLNEQLKNLRTDLDNAHTDIRSLRDKEEQWDSSRF---------QLETKMRESDSDTNKYQLQIASFESERQILTEKIKELDGALRLSDSKVQDMKDDTDKLRRDLTKAE---SVENELRKTIDIQSKTSHEYQL--LKDQLLNTQNELNGANNRKQQLENELLNVRSEVRDYKQRVHDVNNRVSELQRQLQDANTEKNRVEDRFLSVEKVVNTMRTTETDLRQQLETAKNEKRVATKELEDLKRRLAQLENERRNSSQLSDGWKKEKITLLKKIELLENEKRRTDAAIRETALQREAIEKSLNAMERENKELYKNCAQLQQQIAQLEMENGNRILELTNKQREEQERQLIRMRQEKGQIEKVIENRERTHRNRIKQLEDQIAILRDQL 2220
BLAST of Rootletin vs. Ensembl Celegans
Match: lfi-1 (Lin-5 (Five) Interacting protein [Source:UniProtKB/TrEMBL;Acc:H2L0I3]) HSP 1 Score: 91.2781 bits (225), Expect = 7.975e-18 Identity = 113/457 (24.73%), Postives = 230/457 (50.33%), Query Frame = 3
Query: 4479 ENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFR------HHDARL---EEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNM-SLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQL 5819
E R + ++ L DAEE+++ ++ RL SA+T L QEE +++ + +R+ M K+ + E++ R L ++L R H D R +EE S + QL+ R + D + ++ + E+E ++L +++ L ++ + K Q ++ D++ L K E S + + +++ S ++H QL L++++ Q L + ++ LE L + + + + K + + + R+ +Q L D+ ++ +E ++ + E +L Q+ + +EK + + ++R LA E E++ + +K++ L K + +E +K +T+ +T +R +++ L +E EN EL + LQ Q+A+ E + + ++E+ ++ R+E ++ +R QIE+ +E RER HR R+K LE+QI+ L+DQL
Sbjct: 1705 ETRYRNIEDNLQDAEEERRALESRLQSAKTLLRSQEEALKQRDEERRQMKSKMVAAELQARGKEAQLRHLNEQLKNLRTDLDNAHTDIRSLRDKEEQWDSSRF---------QLETKMRESDSDTNKYQLQIASFESERQILTEKIKELDGALRLSDSKVQDMKDDTDKLRRDLTKAE---SVENELRKTIDIQSKTSHEYQL--LKDQLLNTQNELNGANNRKQQLENELLNVRSEVRDYKQRVHDVNNRVSELQRQLQDANTEKNRVEDRFLSVEKVVNTMRTTETDLRQQLETAKNEKRVATKELEDLKRRLAQLENERRNSSQLSDGWKKEKITLLKKIELLENEKRRTDAAIRETALQREAIEKSLNAMERENKELYKNCAQLQQQIAQLEMENGNRILELTNKQREEQERQLIRMRQEKGQIEKVIENRERTHRNRIKQLEDQIAILRDQL 2147
BLAST of Rootletin vs. Ensembl Fly
Match: Root (gene:FBgn0039152 transcript:FBtr0339245) HSP 1 Score: 325.865 bits (834), Expect = 3.312e-89 Identity = 489/2036 (24.02%), Postives = 1003/2036 (49.26%), Query Frame = 3
Query: 168 GLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYG-------SPTNNGYGSRS-------------------ENPYDNLDLESTLI------------KLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD----AEDTG----FETVXXXXXXXXXXXXXXXXXXXXXAKTP---------PFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQL--NCRYQQMDKEYKMA-----IQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRS-------QFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEG------LNRQSN-----TLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSL-------GKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLA--EAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFM 6008
LL+ N ELR+RL +E + YRR+L ++ +Q QA LV RLQ K+ QY+++C++LE +M +ET K G PTN S S D +D L+ KLEEE ++ + N L++QL ++ ++N+ LT D+ KL+ +W R+EL +E ++ EE +F DY SEH+ L + R + +R F E++ + + E+ + E N + + + + ++ ++ A+ ++ R L+NQL + +YE E+ R + L L ++LK K D + + ++ E ++ ++R I+Q V+ D++ D AE TG + ++ S + S K+P FA+ T +AVQ+A++K L + +++ K ++ E+ K Q++ +E ++ L ++ + ++++S + +E + + L+ ++ Q E R+ +N E ++ E+++ +LQ+ +++E++++ + +++R +K+ + ++ ED+ SR +E+ ++ +E +N+ L +++L Q++ E + ++ E K++LE +++K + +L+E KLS+ +D+ +++ + ER L + E LK EL ++ L L + ++++ L++ E++ ++L+ + R++ D+ Q+ + R+K AL EEL++ R+ LE++ + + L++ E + K+ E V + E++ + +E++A+L S++E LE+ L+D + E R+ QLE + QE +V++E+L + RL++E + K ++ QL R + D K+A ++ H E++ +L R+ E ++ + +DD I+ ++ + + ++ ++ +++L+ AE+EKQQ+L + ++K A+ E+L +L++ + LDR ++E++ R E+ + IA L E+ R+ + E+ + E+++ SL + L + + +L +++ E+ ++ E+ + RK KE E + L++E+ + +R L + E++ SN+ LR +K VE+ + + R + ++ QK++ ++ + + E L LK+ E ++L + +++ E + + +LQ ++ + E ++ A++E +K+++ + EA+ A R++L A+ E+ +++++ + +E R ++L D +++L+ EL+ L GAEGR+ AL+ ++ VE K++ E KL+S+ +LRR+ G + + V + R S ++ F SP G +RS S C P+D++P+ VR +R + Q+ ER++DD +Q + + +L++ +Q + +L +LQ L + +E+K ++ +A+ EE ++ + + + E+L+ + A+ E Q E+RL K R ++L+ E +Q ++ + E R ++L+L + ++EGDL R++MAL +K+ + +R+ + + + LE + +L+ T+D++ L K+ + + +++ L LS + ++K+ +QK+L ++E ++R+L ERL+S++ L+E + ++ Q +Q + D E +RS LE N++S T E+EL+++ + EK+ L+ ++ ++Q + E +++ K G DR+ S G+ + + + LD + +E E +L+ +V L+ LA E+E A C M N + ++ + R++ + E+ L+ RE++HRQ+V LE QIS L++QL E +RR Y + K+N+ LR L SL V++ P +D LLE+E+RRLD ++ M
Sbjct: 90 ALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQANLVSRLQSKIQQYRQRCSDLEERM----HETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLPCSSSLDSPPPSCSRDYVD--DVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEESNRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAVKRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDELQNQL-------ATLKVQYESARHEIMERDQRLLELMNQLK----------------KLEDRCAQAESQAALASRYSDEIERLNNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKSPRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQLLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSSDYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETISLREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNNSCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSAGEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETNSRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATEERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKIANLQACAEEAAKRHGEEILQL-------RNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLESLVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDEKQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSE-------ELKMEIRLKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRDHVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNATKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQELGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKARLGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSP------------------SRRF------------SPSRSCGDYD------NRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKKQLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQMLRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQRVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKSAVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNLNELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGEEELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKS--KFSGGNAYDRAEKSNSFYGGAAESGEFDSNRYDVGGGNAGGGSFNCGLDHSV--IEQETRDLRLKVRRLETLLAEKESELARCKARM--NDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRRQQ--YILRSSKANR--EMQHLRSTLGDSLRNVSQHP-VDPHLLESESRRLDSAVSM 2035
BLAST of Rootletin vs. Ensembl Fly
Match: Root (gene:FBgn0039152 transcript:FBtr0339244) HSP 1 Score: 325.865 bits (834), Expect = 3.312e-89 Identity = 489/2036 (24.02%), Postives = 1003/2036 (49.26%), Query Frame = 3
Query: 168 GLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYG-------SPTNNGYGSRS-------------------ENPYDNLDLESTLI------------KLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD----AEDTG----FETVXXXXXXXXXXXXXXXXXXXXXAKTP---------PFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQL--NCRYQQMDKEYKMA-----IQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRS-------QFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEG------LNRQSN-----TLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSL-------GKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLA--EAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFM 6008
LL+ N ELR+RL +E + YRR+L ++ +Q QA LV RLQ K+ QY+++C++LE +M +ET K G PTN S S D +D L+ KLEEE ++ + N L++QL ++ ++N+ LT D+ KL+ +W R+EL +E ++ EE +F DY SEH+ L + R + +R F E++ + + E+ + E N + + + + ++ ++ A+ ++ R L+NQL + +YE E+ R + L L ++LK K D + + ++ E ++ ++R I+Q V+ D++ D AE TG + ++ S + S K+P FA+ T +AVQ+A++K L + +++ K ++ E+ K Q++ +E ++ L ++ + ++++S + +E + + L+ ++ Q E R+ +N E ++ E+++ +LQ+ +++E++++ + +++R +K+ + ++ ED+ SR +E+ ++ +E +N+ L +++L Q++ E + ++ E K++LE +++K + +L+E KLS+ +D+ +++ + ER L + E LK EL ++ L L + ++++ L++ E++ ++L+ + R++ D+ Q+ + R+K AL EEL++ R+ LE++ + + L++ E + K+ E V + E++ + +E++A+L S++E LE+ L+D + E R+ QLE + QE +V++E+L + RL++E + K ++ QL R + D K+A ++ H E++ +L R+ E ++ + +DD I+ ++ + + ++ ++ +++L+ AE+EKQQ+L + ++K A+ E+L +L++ + LDR ++E++ R E+ + IA L E+ R+ + E+ + E+++ SL + L + + +L +++ E+ ++ E+ + RK KE E + L++E+ + +R L + E++ SN+ LR +K VE+ + + R + ++ QK++ ++ + + E L LK+ E ++L + +++ E + + +LQ ++ + E ++ A++E +K+++ + EA+ A R++L A+ E+ +++++ + +E R ++L D +++L+ EL+ L GAEGR+ AL+ ++ VE K++ E KL+S+ +LRR+ G + + V + R S ++ F SP G +RS S C P+D++P+ VR +R + Q+ ER++DD +Q + + +L++ +Q + +L +LQ L + +E+K ++ +A+ EE ++ + + + E+L+ + A+ E Q E+RL K R ++L+ E +Q ++ + E R ++L+L + ++EGDL R++MAL +K+ + +R+ + + + LE + +L+ T+D++ L K+ + + +++ L LS + ++K+ +QK+L ++E ++R+L ERL+S++ L+E + ++ Q +Q + D E +RS LE N++S T E+EL+++ + EK+ L+ ++ ++Q + E +++ K G DR+ S G+ + + + LD + +E E +L+ +V L+ LA E+E A C M N + ++ + R++ + E+ L+ RE++HRQ+V LE QIS L++QL E +RR Y + K+N+ LR L SL V++ P +D LLE+E+RRLD ++ M
Sbjct: 90 ALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQANLVSRLQSKIQQYRQRCSDLEERM----HETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLPCSSSLDSPPPSCSRDYVD--DVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEESNRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAVKRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDELQNQL-------ATLKVQYESARHEIMERDQRLLELMNQLK----------------KLEDRCAQAESQAALASRYSDEIERLNNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKSPRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQLLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSSDYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETISLREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNNSCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSAGEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETNSRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATEERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKIANLQACAEEAAKRHGEEILQL-------RNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLESLVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDEKQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSE-------ELKMEIRLKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRDHVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNATKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQELGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKARLGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSP------------------SRRF------------SPSRSCGDYD------NRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKKQLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQMLRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQRVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKSAVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNLNELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGEEELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKS--KFSGGNAYDRAEKSNSFYGGAAESGEFDSNRYDVGGGNAGGGSFNCGLDHSV--IEQETRDLRLKVRRLETLLAEKESELARCKARM--NDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRRQQ--YILRSSKANR--EMQHLRSTLGDSLRNVSQHP-VDPHLLESESRRLDSAVSM 2035
BLAST of Rootletin vs. Ensembl Fly
Match: Root (gene:FBgn0039152 transcript:FBtr0304161) HSP 1 Score: 325.865 bits (834), Expect = 3.312e-89 Identity = 489/2036 (24.02%), Postives = 1003/2036 (49.26%), Query Frame = 3
Query: 168 GLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYG-------SPTNNGYGSRS-------------------ENPYDNLDLESTLI------------KLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD----AEDTG----FETVXXXXXXXXXXXXXXXXXXXXXAKTP---------PFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQL--NCRYQQMDKEYKMA-----IQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRS-------QFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEG------LNRQSN-----TLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSL-------GKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLA--EAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFM 6008
LL+ N ELR+RL +E + YRR+L ++ +Q QA LV RLQ K+ QY+++C++LE +M +ET K G PTN S S D +D L+ KLEEE ++ + N L++QL ++ ++N+ LT D+ KL+ +W R+EL +E ++ EE +F DY SEH+ L + R + +R F E++ + + E+ + E N + + + + ++ ++ A+ ++ R L+NQL + +YE E+ R + L L ++LK K D + + ++ E ++ ++R I+Q V+ D++ D AE TG + ++ S + S K+P FA+ T +AVQ+A++K L + +++ K ++ E+ K Q++ +E ++ L ++ + ++++S + +E + + L+ ++ Q E R+ +N E ++ E+++ +LQ+ +++E++++ + +++R +K+ + ++ ED+ SR +E+ ++ +E +N+ L +++L Q++ E + ++ E K++LE +++K + +L+E KLS+ +D+ +++ + ER L + E LK EL ++ L L + ++++ L++ E++ ++L+ + R++ D+ Q+ + R+K AL EEL++ R+ LE++ + + L++ E + K+ E V + E++ + +E++A+L S++E LE+ L+D + E R+ QLE + QE +V++E+L + RL++E + K ++ QL R + D K+A ++ H E++ +L R+ E ++ + +DD I+ ++ + + ++ ++ +++L+ AE+EKQQ+L + ++K A+ E+L +L++ + LDR ++E++ R E+ + IA L E+ R+ + E+ + E+++ SL + L + + +L +++ E+ ++ E+ + RK KE E + L++E+ + +R L + E++ SN+ LR +K VE+ + + R + ++ QK++ ++ + + E L LK+ E ++L + +++ E + + +LQ ++ + E ++ A++E +K+++ + EA+ A R++L A+ E+ +++++ + +E R ++L D +++L+ EL+ L GAEGR+ AL+ ++ VE K++ E KL+S+ +LRR+ G + + V + R S ++ F SP G +RS S C P+D++P+ VR +R + Q+ ER++DD +Q + + +L++ +Q + +L +LQ L + +E+K ++ +A+ EE ++ + + + E+L+ + A+ E Q E+RL K R ++L+ E +Q ++ + E R ++L+L + ++EGDL R++MAL +K+ + +R+ + + + LE + +L+ T+D++ L K+ + + +++ L LS + ++K+ +QK+L ++E ++R+L ERL+S++ L+E + ++ Q +Q + D E +RS LE N++S T E+EL+++ + EK+ L+ ++ ++Q + E +++ K G DR+ S G+ + + + LD + +E E +L+ +V L+ LA E+E A C M N + ++ + R++ + E+ L+ RE++HRQ+V LE QIS L++QL E +RR Y + K+N+ LR L SL V++ P +D LLE+E+RRLD ++ M
Sbjct: 90 ALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQANLVSRLQSKIQQYRQRCSDLEERM----HETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLPCSSSLDSPPPSCSRDYVD--DVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEESNRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAVKRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDELQNQL-------ATLKVQYESARHEIMERDQRLLELMNQLK----------------KLEDRCAQAESQAALASRYSDEIERLNNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKSPRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQLLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSSDYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETISLREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNNSCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSAGEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETNSRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATEERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKIANLQACAEEAAKRHGEEILQL-------RNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLESLVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDEKQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSE-------ELKMEIRLKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRDHVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNATKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQELGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKARLGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSP------------------SRRF------------SPSRSCGDYD------NRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKKQLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQMLRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQRVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKSAVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNLNELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGEEELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKS--KFSGGNAYDRAEKSNSFYGGAAESGEFDSNRYDVGGGNAGGGSFNCGLDHSV--IEQETRDLRLKVRRLETLLAEKESELARCKARM--NDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRRQQ--YILRSSKANR--EMQHLRSTLGDSLRNVSQHP-VDPHLLESESRRLDSAVSM 2035
BLAST of Rootletin vs. Ensembl Zebrafish
Match: crocc2 (ciliary rootlet coiled-coil, rootletin family member 2 [Source:ZFIN;Acc:ZDB-GENE-131121-625]) HSP 1 Score: 606.29 bits (1562), Expect = 0.000e+0 Identity = 604/1911 (31.61%), Postives = 1054/1911 (55.15%), Query Frame = 3
Query: 210 EEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYD---NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLAS-EHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATR-VLIEDTESMHQALRNISQVVLNDS--DYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQ-----SKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIP--RTGSPSSKRRQLSRSPSN---------------CDFNPV-----DINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
EE YR KL +Q+SQ +QAQLVQ+LQ K+LQYK++C LE ++ E+ K + ++ + ++ +LD+++ L L++EQK+ + L++ N L+EQL QA ++NQ LT E Q++R+E EQR+ R E + N S E +++L R R F +L+A +R +++L+ E+ + L+ AC NL E+ + S S LE QLR+K RE Q R++ E EL R+ EL + +L+ QN EK+ L AL+ N + ED+A R L + S+HQ L NI+QVV + D + F + + S + P AVQ A+ ++ Q ++L++ L +S+E ++Q+ E++ R LE ++ ++ E + L R ++DS R+A+R++N+L I ++E+ +LE+ S++ Q+E TE E L+S++ ++QR R+ L ++ED ++ L R+ ESER + +++ LE + S ++++V KEA+++ L+KEVL E + ALS+ E AE E+ + K ++ + L+++++K+ + E L + E +R + Q E E++ L +R E E+ ++EL R +QE L L+ EK L+ L+ E + L REK + EQ +QK A+ E+L C RE+E S + KE++ L ++K L VQ+T+ +R+++ ++ +A+L ++RE LE++L D Q+L LE +++L+GEN L ++L EL ++ E + + + + ++ L + Q ++ + + + SH E + K E +RE+ + E ++ + Q++ +++ +Q+ +SLL AE+ KQQ LS E EK + E++I + ++++ E DR++++ ++QEQDK +GL +EL + RS+FE+ + E+ +SLN I++L+ A +E L L EE+RD R+E++E +R+L+E + K+ +RE +LKR L + REKE + SN LR +K VE E L R + Q++ V +E + +QEV +LR N++++EK+RL +RELQELRR +K L+ + + +E++D+Q R+ ++E++E+E R+E F L+QKV+E+EA +EA K+ A QRHI +LE Q + RD +S+ +S + ++A +LQ+ A E ++++ AE R LE +A+ + ++++E +L ++S+LRR +G R+ G+ SP P R SPS R +RSP C +P +++ +AV++ L F Q+L D +++RD++ AQ SL +++ + E + +Q L+Q++ + EE K+ + L Q +L++++E ++R E + M E+L+ ++ +E+E + L+D+L + + + LE E ++ K+ E + R +L L +R LEG+LQR ++ T+ E E L +R+N+L +++ D E ++ L++ +R++ ALA E + S KDK+Q L SL+ + +++ QLQ+AL +SE ++++++E+L+ ++ AL ++ QN+ L ER+Q +Q + E + SEL+ R + QRQ+AEQE + L + + LQE+V ++Q + EK ++ + +L KDRS+LK++L KVE + K E ER + +Q +R LE+E E Q ++ LQ+Q++ E H L+E+++RH+QE+ +E E +R S Q ER LE RE+AHRQRV LEEQ+STLK+QL EM+RR
Sbjct: 126 EEGRSYRLKLQAYQESQHRQAQLVQKLQAKLLQYKKRCGNLEQQVLEKTSESEKLRLSLQSHLDSSAHRLQRFEQEHSLDVQNKLALLDDEQKRCSGLSQVNALLREQLEQANETNQALT-------ESLQKARDEAEQRDTRLRRE-LEMNTSRNSREQVRVRSLWRQVAFLRSSFTQLRAFADRSLSDLQAEYAATSHRLRVACLNL-------EASKSQSCTTASNGVSALETQLRDKLREAMQLQARFDAEKVELSSRILELGEMVKQLRAQNSEKESNLIALR--SNLNKMETNRSEDRAERDALRSEISSLHQILHNINQVVSGEVSRDCSSKHEHSFISAGSISSSQ---------------REGPLI-----AVQKALLQRQTQTEDLQACLEASQEQVVTLQKQLHVEEDKTRTLEKRLQELQQENQLLERAEEDSTRDAERYRNSLGIITSEKGELERQLSAMQQQLECTQTEQEGLRSSSLDVQRQRDLLRQQREDLERQLARERSESERGRHTLEQLEARYSDVRKELVTLKEALSQLSLQKEVLEDENRSLAQALSKAEAQNAEQELALTKLRSEDAGLQDSLAKMGALSEGLAKDKVELNRLLLQAEAEKAELGERRREAELERMAAREELARLQQELLDLTAEKRALEASHNYLQEAKMTLDAEYNLLQREKSSVQEQHAQASKQKQAVQEQLNACHREMELSAALLQKTYKERDELARDKANLTVQLTSEQRKSKALAQDLAALRAERESLESSLLDRQELASSLEAIRDRLDGENASLQQANKSLTRELSSVQSELEQQMSRALQEKQALELKLAQAERNAQEMLITTQQSHQEQLHTERKHKERMHAELCAQREQAESQLRAECKELRIQSRRVLQQLQEDLAKLQQHCTESLLQAESHKQQVLSEKEAEKATLIERIITLERDMEAAALEKDRMRRDFLSKQEQDKDTASGLQAELHNLRSRFEESLSSREVSERSLNEQIRELSTQWQGAHQEAERLKRELSEVEESRDTGRKELIEAHRELRECLQDKDEQRREGLDLKRALGDAVREKEAINTSNHELRTALKRVEFENNSLKRVGEEKDQRLTVLEECKSSLQQEVLKLRTNMRELEKSRLQARRELQELRRQVKTLEGQCTQQGQELSDMQARLTQEEQREEEMRKETFTLRQKVLESEAGKEAALKEAAGLQRHIAELEEAHQQEKELQQQRD-ASLQDSQLKQREEVARLEGALQESQ----AQLREFGLSVNLAEARAQGLEEQLAQNDAKRRDLEHRLNVLNSTLRRTLGSRR--------------------------------------------GRQSPTPGERGRSPSPWR---TRSPVKGEIISDETGVGGLPVCSLSPSRREDGELDLDAVQMLLHSFQQELRDTQKERDESHAQVHSLSKQVENLQANQERSNTCIQMLRQSIRELEEGKRELAKHLEGMQASLVLRDEAVQRGETQNRIMNEELAKMKKSQLIIETEAKALQDKLERSQASEESLEAERRRLKETCEAVDARAARLDLKRRGLEGELQRAQLRTTEMEAEASSLQERLNNLRRELGDSEERAALLKVECERLATALAHKELQESQLKDKLQALTASLTDSNASAGTARDQAVQLQRALSASEHNQKMVQEKLDKTREALTHSRKQNHDLSERLQSLQRSQEECELKVSELDKQTRVLQETVRQRQQAEQEAQESCLLLQEQNSQLQEQVATLQTTAHRLQGEKSDLERTLLRLGKDRSSLKRTLEKVEHNRQKKEVEILHGEREREQQEQKIRNLEEELNETQAEIHTLQSQISILEHTHSQRLLEVSARHKQELDLESERMRDSQQQTERALEAREKAHRQRVHCLEEQVSTLKEQLEQEMKRR 1947
BLAST of Rootletin vs. Ensembl Zebrafish
Match: crocc2 (ciliary rootlet coiled-coil, rootletin family member 2 [Source:ZFIN;Acc:ZDB-GENE-131121-625]) HSP 1 Score: 293.893 bits (751), Expect = 7.428e-82 Identity = 262/893 (29.34%), Postives = 486/893 (54.42%), Query Frame = 3
Query: 2367 KEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQ-----SKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIP--RTGSPSSKRRQLSRSPSN---------------CDFNPV-----DINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMS 4964
KE++ L ++K L VQ+T+ +R+++ ++ +A+L ++RE LE++L D Q+L LE +++L+GEN L ++L EL ++ E + + + + ++ L + Q ++ + + + SH E + K E +RE+ + E ++ + Q++ +++ +Q+ +SLL AE+ KQQ LS E EK + E++I + ++++ E DR++++ ++QEQDK +GL +EL + RS+FE+ + E+ +SLN I++L+ A +E L L EE+RD R+E++E +R+L+E + K+ +RE +LKR L + REKE + SN LR +K VE E L R + Q++ V +E + +QEV +LR N++++EK+RL +RELQELRR +K L+ + + +E++D+Q R+ ++E++E+E R+E F L+QKV+E+EA +EA K+ A QRHI +LE Q + RD +S+ +S + ++A +LQ+ A E ++++ AE R LE +A+ + ++++E +L ++S+LRR +G R+ G+ SP P R SPS R +RSP C +P +++ +AV++ L F Q+L D +++RD++ AQ SL +++ + E + +Q L+Q++ + EE K+ + L Q +L++++E ++R E + M E+L+ ++ +E+E + L+D+L + + + LE E ++ K+ E + R +L L +R LEG+LQR ++ T+ E E L +R+N+L +++ D E ++ L++ + +S
Sbjct: 129 KERDELARDKANLTVQLTSEQRKSKALAQDLAALRAERESLESSLLDRQELASSLEAIRDRLDGENASLQQANKSLTRELSSVQSELEQQMSRALQEKQALELKLAQAERNAQEMLITTQQSHQEQLHTERKHKERMHAELCAQREQAESQLRAECKELRIQSRRVLQQLQEDLAKLQQHCTESLLQAESHKQQVLSEKEAEKATLIERIITLERDMEAAALEKDRMRRDFLSKQEQDKDTASGLQAELHNLRSRFEESLSSREVSERSLNEQIRELSTQWQGAHQEAERLKRELSEVEESRDTGRKELIEAHRELRECLQDKDEQRREGLDLKRALGDAVREKEAINTSNHELRTALKRVEFENNSLKRVGEEKDQRLTVLEECKSSLQQEVLKLRTNMRELEKSRLQARRELQELRRQVKTLEGQCTQQGQELSDMQARLTQEEQREEEMRKETFTLRQKVLESEAGKEAALKEAAGLQRHIAELEEAHQQEKELQQQRD-ASLQDSQLKQREEVARLEGALQESQ----AQLREFGLSVNLAEARAQGLEEQLAQNDAKRRDLEHRLNVLNSTLRRTLGSRR--------------------------------------------GRQSPTPGERGRSPSPWR---TRSPVKGEIISDETGVGGLPVCSLSPSRREDGELDLDAVQMLLHSFQQELRDTQKERDESHAQVHSLSKQVENLQANQERSNTCIQMLRQSIRELEEGKRELAKHLEGMQASLVLRDEAVQRGETQNRIMNEELAKMKKSQLIIETEAKALQDKLERSQASEESLEAERRRLKETCEAVDARAARLDLKRRGLEGELQRAQLRTTEMEAEASSLQERLNNLRRELGDSEERAALLKVCVCVLS 969
BLAST of Rootletin vs. Ensembl Zebrafish
Match: si:dkey-230p4.1 (si:dkey-230p4.1 [Source:ZFIN;Acc:ZDB-GENE-081104-380]) HSP 1 Score: 87.4261 bits (215), Expect = 2.021e-16 Identity = 90/277 (32.49%), Postives = 144/277 (51.99%), Query Frame = 3
Query: 201 RLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQ--------ARCLQTACSNLSCNMKSAESKQQLSTERE--SRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTV 1001
RL+EE E+R+KL ++ + +QA L+ +LQ KVL Y+ +C LE + G R + LESTLI+LEEEQ+++ L+E + L+ QL+Q+T++N+ L D+ KL+ +W ++ EE+ Q+E W+ E+ Y+ EHS L L + RRQ LK +T++++ LK +F+R A L + ++ + S Q LST + S D LN E K+ EL + E E EL++R+ EL +
Sbjct: 61 RLDEEQREWRKKLREGREREARQALLIHKLQHKVLDYRARCQALEQQA-----------VGEERELRIRERILRDERSDTLESTLIRLEEEQQRSLGLSEVSALLRSQLSQSTEANEALRDDLRKLTADWSKAVEEVVQKEIDWQKEKEVLTGYIGKEHSRLMTLWGRVVTLRRQCHSLKTATDKDLWELKADFSRLSSSLLSVWASPLSAPKLTHNSSLSQSTSAQPLSTTQGPLSLDDLNHEQTDERKSSEL---KADIESETLELRNRITELNM 323
BLAST of Rootletin vs. Ensembl Zebrafish
Match: si:dkey-230p4.1 (si:dkey-230p4.1 [Source:ZFIN;Acc:ZDB-GENE-081104-380]) HSP 1 Score: 81.2629 bits (199), Expect = 2.983e-16 Identity = 65/184 (35.33%), Postives = 107/184 (58.15%), Query Frame = 3
Query: 201 RLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTE 752
RL+EE E+R+KL ++ + +QA L+ +LQ KVL Y+ +C LE + G+ + + + RS+ LESTLI+LEEEQ+++ L+E + L+ QL+Q+T++N+ L D+ KL+ +W ++ EE+ Q+E W+ E+ Y+ EHS L L + RRQ LK +T+
Sbjct: 61 RLDEEQREWRKKLREGREREARQALLIHKLQHKVLDYRARCQALEQQA-VGEERELRIRERILRDE----RSDT------LESTLIRLEEEQQRSLGLSEVSALLRSQLSQSTEANEALRDDLRKLTADWSKAVEEVVQKEIDWQKEKEVLTGYIGKEHSRLMTLWGRVVTLRRQCHSLKTATD 233
BLAST of Rootletin vs. Ensembl Xenopus
Match: pgm2 (phosphoglucomutase 2 [Source:Xenbase;Acc:XB-GENE-1006723]) HSP 1 Score: 693.73 bits (1789), Expect = 0.000e+0 Identity = 625/1926 (32.45%), Postives = 1090/1926 (56.59%), Query Frame = 3
Query: 168 GLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYD---NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQ--QLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLND--SDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRR--DKDDSFREA-----------------------DRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQ---LNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
G + L+LR+R EE Y+RKL +Q+ QQ+QAQLVQRLQ KVLQYK +C ELE ++ E+ + + N D +L+L + L +L+EEQ++++ L + N L+EQ+ Q +N+ LT +I +L+ + +S E+L+QRE WR E ++ YL++EHS + L R R QF++++ TER + +K + +R +R + TAC NL N++ ++S L T R LE QLR+K R++ Q++++ E EL RV EL++ ++KL +N +K++ L+ DT E+ + R E+ +S+ Q L +I+Q+ D D E TG + + + +P F ++ S I ++S + + ++ N ++ D E E L +++ A RD +E ++ +K+ EA R +N++ + + E+ +E+ ++ + E L E ERLK+ N EL R REALE+++E+ ++ +R KE ER QR + LE+K+S K+D+V +E++++ LE++VL EK+ + ALS+ E S+AELE+ +N+ + L ++++K+ + E L + +R I QLE ER++L ++R E + LK++ E E L ++ +R + E +R EE+L +++E+ L +Q+ L ++L + RRE++ +++ ++ E LT + EL VQ+ A E E ++ E A L + +E LE+TLY+ Q+ QLE R+EQLEGE L + K++L E RQ + E E + R+Q + + Q ++ ++++ + H + V+RL + ES R ++++ + R E++D++ + E KQ+++ EI+ +Q ER++SLL E+EKQ++L L E EK +++EKL+ TQ EL + E++R ++E+ +QEQDK ++ + SELK+ +S FED H E K+L +K L+ ++ KEV +L T L++AE++RD+LRR+I+E+ R L+E EE E ++E EL+R + + RE+E + SN LR IK +E+ER L + + +Q+++V E +++E LR L+DVE++ ++ +R+LQELRR +K L E + +EV +LQ R+ ++E+KE ++RRE+ L+Q++ E+E+ REA R+++ Q+H+ LE+ + ++++ + SR ++L +A SLQ+ +++ + R+ L E RV ALE+ +++ + +++E E + ++S+LRR IG R + R+ ++ TKG D+++ + +G+ +P R SP SRSP P ++ PE V+ ALR+F+Q+L D +R+RD+A Q SL +L++ E +++ Q+LQ+ L D +E ++G + RL +A T + + ++ +R +E +R+A E++ + + E E R +DRL++ R+ + R E E K ++ +E +ENR T+L+L KRSLEG+L+R +M+L ++E E + L +R + L +Q++D E + SLQ + R+++AL++ ++ ++K Q L +L + LQ +S+LQ AL ++EQDRR+L+ERL+S + AL ++K N L + IQ Q L + EL+ + ++++Q+ E+E + +QE++ +Q + A E +KQEI + LEK + L+ +L KV+R++L+ E A + E+ +L + L + E QRQ LQ Q+ E E++H LME++ RHRQE+ E + Q+E TLE RER+HRQRV+GLEEQI++L++QL E++RR
Sbjct: 126 GETEAVLDLRQRFHEEEMAYKRKLSAYQEGQQRQAQLVQRLQDKVLQYKRRCRELEQQLLEKTSESEQQRLALQARLDSAETKLNRMDQNHSLELNNALTRLDEEQQRSSSLAQVNNTLREQMEQLKTTNEKLTEEIKRLTSDLHKSHEDLDQREAEWRKERQNYTFYLSNEHSGILTLWRKAAALRSQFLDMRGITERGLAEIKTDISRSSRRMHTACLNLDSNLRLSQSAAGASLHTAR-------LEQQLRDKVRQMMQLQSQWDAEKVELNSRVSELSLQTEKLVRENSKKEKEKQILEN-SGCDTLQEL----EGVR---EECDSLQQVLSSITQMCQTDVSPDPTCVESTGVSATNEMRQ----------------SVSPLFVFSS-----SPIRRRSPTRMRSPQRSELALKAVQNVLQRHQDREKE---LHSELAAARDSLEQTKKQQEKNQHVSEAHTEELKKENKEINTILESVKQELQRCQNSIELLTREKEDMEESLQTIKQKAEGSLLETERLKAVNAELLRQREALEEQKEELNRERDRSQKELERGQRIQEQLEEKVSILKKDLVTVRESLSQTALERDVLQGEKEAVASALSKAESSRAELELALNQMHSERATLTDSLAKMGALNEGLAQDKVALNRVILQLEGERAALHEDRRAGEANCAALKEQQEALEHEVSQLQADRVGLERRCREAEEKHKRQEEQLLGVHKERGKLQDQLAQANTLVQTLQDQLKESRREMDLQGSALQRAAQRMEELTSQNAELGVQLAALEDERQEHEEEFAQLRAQKESLESTLYETQRRAAQLEDRREQLEGEIHTLTLVKQSLQVETQ--RQALEAE-EALAGLRQQKVTVELQLAQTEQAAQLSLSNQSQQHQDTVDRLKREKESLRLTMIADKQDSVQRLEKEKEDLLFDRENVKQKLSAEILRLQEEREESLLKVESEKQKALLLKETEKNSLSEKLMNTQRELSDTKMEMERCRREAQIKQEQDKTSLDNVLSELKALQSDFEDAVSAHSKENKNLRDRMKQLSQERESLNKEVEELRTQLRLAEDSRDSLRRDIIEVQRLLREKEEICESQRKETLELRRDVGDLTRERETLQKSNTELRACIKKIESERSSLQVAMEEKQQRISVLQEGKSCAEREAAHLRATLRDVERSHIEARRQLQELRRQVKTLGGESSQKEQEVAELQARIQQEEQKEQQSRRESLELRQRITESESEREAARREILTLQQHLSALESSSRQREKELEQRLSESRAGEQRLQEACKSLQEQLQRCTSESGDSRLQLGAFEARVRALEAELSKSQASRREAESHVNGLYSALRRTIGVRGRSPSPRRALSS--------------------TKGLDSSHGSPMEGRVTPSSRGVSPDR-----SRSP-----YP-ELEPELVKQALRDFMQELRDTQRERDEAGIQVLSLRRQLEDSINSCERLQSQQQKLQRQLADLQEGQRGTEERLGTAHTEMRLLQDNLRHSEAERQASGERIMELEHSLRICEDENRDFQDRLSRARNAENRQELECKGLREVLEASENRGTELELRKRSLEGELERTRMSLAEREAEVQTLQERAHHLQEQLKDSEDREASLQTEVQRLNLALSRAQDGERQLQEKSQGLTQALGEATAGRGNLQEDVSRLQGALTAAEQDRRVLQERLDSVRQALSDSKRHNLRLSQTIQEQQQQLEEMHLNCRELQAQLQDLQQTLIKQQQREEEQGAALLE-------VQEKLKMLQGDKAQVELDKQEIQHSAVLLEKGNNTLRATLDKVKREQLRIEGEAQRLSVEKEQLSHSVISLRKDLAEAQRQNQYLQEQMTEMERSHTQRLMEVSLRHRQELDSESNRAHSIQQQVESTLESRERSHRQRVRGLEEQINSLQEQLEQEVRRR 1971
BLAST of Rootletin vs. Ensembl Xenopus
Match: pgm2 (phosphoglucomutase 2 [Source:Xenbase;Acc:XB-GENE-1006723]) HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0 Identity = 625/1926 (32.45%), Postives = 1090/1926 (56.59%), Query Frame = 3
Query: 168 GLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYD---NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQ--QLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLND--SDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRR--DKDDSFREA-----------------------DRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQ---LNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
G + L+LR+R EE Y+RKL +Q+ QQ+QAQLVQRLQ KVLQYK +C ELE ++ E+ + + N D +L+L + L +L+EEQ++++ L + N L+EQ+ Q +N+ LT +I +L+ + +S E+L+QRE WR E ++ YL++EHS + L R R QF++++ TER + +K + +R +R + TAC NL N++ ++S L T R LE QLR+K R++ Q++++ E EL RV EL++ ++KL +N +K++ L+ DT E+ + R E+ +S+ Q L +I+Q+ D D E TG + + + +P F ++ S I ++S + + ++ N ++ D E E L +++ A RD +E ++ +K+ EA R +N++ + + E+ +E+ ++ + E L E ERLK+ N EL R REALE+++E+ ++ +R KE ER QR + LE+K+S K+D+V +E++++ LE++VL EK+ + ALS+ E S+AELE+ +N+ + L ++++K+ + E L + +R I QLE ER++L ++R E + LK++ E E L ++ +R + E +R EE+L +++E+ L +Q+ L ++L + RRE++ +++ ++ E LT + EL VQ+ A E E ++ E A L + +E LE+TLY+ Q+ QLE R+EQLEGE L + K++L E RQ + E E + R+Q + + Q ++ ++++ + H + V+RL + ES R ++++ + R E++D++ + E KQ+++ EI+ +Q ER++SLL E+EKQ++L L E EK +++EKL+ TQ EL + E++R ++E+ +QEQDK ++ + SELK+ +S FED H E K+L +K L+ ++ KEV +L T L++AE++RD+LRR+I+E+ R L+E EE E ++E EL+R + + RE+E + SN LR IK +E+ER L + + +Q+++V E +++E LR L+DVE++ ++ +R+LQELRR +K L E + +EV +LQ R+ ++E+KE ++RRE+ L+Q++ E+E+ REA R+++ Q+H+ LE+ + ++++ + SR ++L +A SLQ+ +++ + R+ L E RV ALE+ +++ + +++E E + ++S+LRR IG R + R+ ++ TKG D+++ + +G+ +P R SP SRSP P ++ PE V+ ALR+F+Q+L D +R+RD+A Q SL +L++ E +++ Q+LQ+ L D +E ++G + RL +A T + + ++ +R +E +R+A E++ + + E E R +DRL++ R+ + R E E K ++ +E +ENR T+L+L KRSLEG+L+R +M+L ++E E + L +R + L +Q++D E + SLQ + R+++AL++ ++ ++K Q L +L + LQ +S+LQ AL ++EQDRR+L+ERL+S + AL ++K N L + IQ Q L + EL+ + ++++Q+ E+E + +QE++ +Q + A E +KQEI + LEK + L+ +L KV+R++L+ E A + E+ +L + L + E QRQ LQ Q+ E E++H LME++ RHRQE+ E + Q+E TLE RER+HRQRV+GLEEQI++L++QL E++RR
Sbjct: 121 GETEAVLDLRQRFHEEEMAYKRKLSAYQEGQQRQAQLVQRLQDKVLQYKRRCRELEQQLLEKTSESEQQRLALQARLDSAETKLNRMDQNHSLELNNALTRLDEEQQRSSSLAQVNNTLREQMEQLKTTNEKLTEEIKRLTSDLHKSHEDLDQREAEWRKERQNYTFYLSNEHSGILTLWRKAAALRSQFLDMRGITERGLAEIKTDISRSSRRMHTACLNLDSNLRLSQSAAGASLHTAR-------LEQQLRDKVRQMMQLQSQWDAEKVELNSRVSELSLQTEKLVRENSKKEKEKQILEN-SGCDTLQEL----EGVR---EECDSLQQVLSSITQMCQTDVSPDPTCVESTGVSATNEMRQ----------------SVSPLFVFSS-----SPIRRRSPTRMRSPQRSELALKAVQNVLQRHQDREKE---LHSELAAARDSLEQTKKQQEKNQHVSEAHTEELKKENKEINTILESVKQELQRCQNSIELLTREKEDMEESLQTIKQKAEGSLLETERLKAVNAELLRQREALEEQKEELNRERDRSQKELERGQRIQEQLEEKVSILKKDLVTVRESLSQTALERDVLQGEKEAVASALSKAESSRAELELALNQMHSERATLTDSLAKMGALNEGLAQDKVALNRVILQLEGERAALHEDRRAGEANCAALKEQQEALEHEVSQLQADRVGLERRCREAEEKHKRQEEQLLGVHKERGKLQDQLAQANTLVQTLQDQLKESRREMDLQGSALQRAAQRMEELTSQNAELGVQLAALEDERQEHEEEFAQLRAQKESLESTLYETQRRAAQLEDRREQLEGEIHTLTLVKQSLQVETQ--RQALEAE-EALAGLRQQKVTVELQLAQTEQAAQLSLSNQSQQHQDTVDRLKREKESLRLTMIADKQDSVQRLEKEKEDLLFDRENVKQKLSAEILRLQEEREESLLKVESEKQKALLLKETEKNSLSEKLMNTQRELSDTKMEMERCRREAQIKQEQDKTSLDNVLSELKALQSDFEDAVSAHSKENKNLRDRMKQLSQERESLNKEVEELRTQLRLAEDSRDSLRRDIIEVQRLLREKEEICESQRKETLELRRDVGDLTRERETLQKSNTELRACIKKIESERSSLQVAMEEKQQRISVLQEGKSCAEREAAHLRATLRDVERSHIEARRQLQELRRQVKTLGGESSQKEQEVAELQARIQQEEQKEQQSRRESLELRQRITESESEREAARREILTLQQHLSALESSSRQREKELEQRLSESRAGEQRLQEACKSLQEQLQRCTSESGDSRLQLGAFEARVRALEAELSKSQASRREAESHVNGLYSALRRTIGVRGRSPSPRRALSS--------------------TKGLDSSHGSPMEGRVTPSSRGVSPDR-----SRSP-----YP-ELEPELVKQALRDFMQELRDTQRERDEAGIQVLSLRRQLEDSINSCERLQSQQQKLQRQLADLQEGQRGTEERLGTAHTEMRLLQDNLRHSEAERQASGERIMELEHSLRICEDENRDFQDRLSRARNAENRQELECKGLREVLEASENRGTELELRKRSLEGELERTRMSLAEREAEVQTLQERAHHLQEQLKDSEDREASLQTEVQRLNLALSRAQDGERQLQEKSQGLTQALGEATAGRGNLQEDVSRLQGALTAAEQDRRVLQERLDSVRQALSDSKRHNLRLSQTIQEQQQQLEEMHLNCRELQAQLQDLQQTLIKQQQREEEQGAALLE-------VQEKLKMLQGDKAQVELDKQEIQHSAVLLEKGNNTLRATLDKVKREQLRIEGEAQRLSVEKEQLSHSVISLRKDLAEAQRQNQYLQEQMTEMERSHTQRLMEVSLRHRQELDSESNRAHSIQQQVESTLESRERSHRQRVRGLEEQINSLQEQLEQEVRRR 1966
BLAST of Rootletin vs. Ensembl Xenopus
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:NCBI gene;Acc:100486658]) HSP 1 Score: 684.1 bits (1764), Expect = 0.000e+0 Identity = 532/1416 (37.57%), Postives = 858/1416 (60.59%), Query Frame = 3
Query: 1413 NFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSEN--------------EREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKD-LTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQ 5615
+ ++Q+ DNENERR LE ++ ++ E + + K+D+ R+ R +N + + E+ LEK +L +++ Q E E+L+ AN +LQR R+ L++E+ED +D ER ++ +R ++ + LE K+S ++++V KE+++K LE+EV E+ ++ ALS+ E AELE+ +N+++T E +L + +SK+S+ E L + E R I QLE ER+ L +K +EQEK+ ++DELVR EQE L L E+ ++ +E N E+L +E+ L +E+ L EQ+G Q ++ ++L+Q RR++E + + S+EKE L K+KG LVVQ+TA ERE+R +E IA L +++E LE L++AQQ +I + +RKEQ L V+ +NL RL +E + +C Y D E N G++N E+EEL+HR ER++++ +E E++EM++EI +Q+ERD+SLL AE EKQQ+LS+ E EK +++EKL Q L + EL+R K++S RQEQD+ I L+SELK R Q E+ H+ E+K LN ++ L L EV +L T L + E+ RD +RRE++E +R+++E +E E K+E EL+R L +E +EKE V SNE LR ++ E+ERI L R + +Q+++V +E S++EVN+LR++L+DVE++RL+ +RELQ+LRR +K LD E K ++++ ++Q R++ E++E+E+RR+NF LKQK++ETEA REA RK+L+ QR + ++E ++R+ ++ +R + +KL D +L+ ++ +L + LS +EGRV LE+ ++R+EG K+E+EFKL S+HS+LRR +G + T + G R+GSP SK + SP+ + DI+PE VR ALR+F+Q+L D ER+RD+ Q + L E E + + A R+QQLQ+ L + EE K+ +DG+L QT L++QEET+RR ER+RK EK ++ +R+ + E E+R +D++NK + ++A+ E E ++ K+ ++ +E+R T+L+L++R LEG+LQR K+ L D+E E + + R+ +L +Q+ D EG+ +LQL ++R++ LAK +E + K+K+Q L +LS + +K++QLQK L SE +RR+L+ERLE+++ A+ E K QN LME+ Q+++ + + E +R+ELEG RQ ++ QRQE+E + QKL E+ LQER+ +QR + E+EK+E+ ++ +LEKD++ALKK+L KVER+KLKT E + E+ LD+ L +E E E QRQV +L+
Sbjct: 36 SLRKQLSDNENERRALEQKIQQLQQERDVMGGAKEDAERDVTRLRNHIEQLNGEKGALEKSLQNLQEELNGQRQENEKLQLANSDLQRQRDLLQEEKEDVCRDRERALQNVDRGHKAQEQLEVKLSALRKELVVVKESLHKCSLEREVSEAERVDVSQALSKAETRLAELELTLNRQRTEEASLRDALSKMSALNESLAQDKTELSRIIAQLEEERAHLQGQKHEVEQEKASIRDELVRLEQEKLELDTERFGLDNSLQAMEQNREKLLQEVQALRKERGQLQEQLG--QVSLLSIRDDLVQSRRDVELQSECLLRASREKEELMKDKGSLVVQLTASERESRALAEEIAVLRTEKEALETALFEAQQQLIHVTSRKEQ-------LEVESQNL-----RLNKES-------LQGTGSFSC-YGSCDHE-------------------------NGQGNDNTLFAQNELRLELESEKEELVHRLTQEREELVARHEMEREEMSEEIAALQQERDESLLQAEFEKQQALSVKESEKASLSEKLSHAQHGLSGLSLELERHKRDSQIRQEQDRGTILALNSELKGLRGQLEEALVLHDRELKGLNDKSREPLGLDLIGCCLEVEELKTQLCVVEDARDAVRRELIEAHRRVREGQELLEGHKKENMELRRALGDEAKEKEAVQKSNEELRGAVRRAESERISLKRSNEEKEQRLSVLEEARGASEKEVNDLRSSLRDVERSRLEARRELQDLRRQLKLLDDESIKRSRDMAEVQARLSTYEQREEESRRDNFTLKQKLMETEAGREAARKELSALQRKVAEVEGEFGVRERELQGSLDEARGNEKKLLDNTRNLEIKLQAAQEESGQLGLRLSASEGRVHGLEAELSRLEGLKREVEFKLGSLHSALRRTLGIGRAG----------------------------------RTPSPAYRG------RSGSPKSKTPERPPSPTRGEQLVADIDPEVVRAALRDFLQELRDTERERDELRTQLGTSNRHLAEMEAERDGAITRVQQLQKVLSECEEAKRNVDGKLGGLQTTLILQEETLRRYERERKLAQEKAASLERSLQAAEGEQRAAQDKMNKIKANEAKYENERRRLKEVLDASESRNTKLELSRRGLEGELQRHKLVLADREAEMQEMQQRMEALQRQLSDSEGRVGTLQLCVERLNSTLAKAQESETSLKEKVQSLTGALSDSNCTSASSHDKLNQLQKVLTGSEHERRILQERLEAARQAVAEGKKQNVALMEQNQVLRDEQAEGELQRAELEGQVRQMQEILRQRQESEGASLRNVQKLQEEREVLQERLCGLQRAVVQLESEKREVERSSMRLEKDKNALKKTLDKVEREKLKTAEDTLRLSAEKGRLDRSLTTVEQELAEAQRQVQLLE 1364
BLAST of Rootletin vs. Ensembl Xenopus
Match: pgm2 (phosphoglucomutase 2 [Source:Xenbase;Acc:XB-GENE-1006723]) HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0 Identity = 613/1923 (31.88%), Postives = 1077/1923 (56.01%), Query Frame = 3
Query: 168 GLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQ--QLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLND--SDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRR--DKDDSFREA-----------------------DRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQ---LNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
G + L+LR+R EE Y+RKL +Q+ QQ+QAQL L+ K+Q ++ +TK N +L+L + L +L+EEQ++++ L + N L+EQ+ Q +N+ LT +I +L+ + +S E+L+QRE WR E ++ YL++EHS + L R R QF++++ TER + +K + +R +R + TAC NL N++ ++S L T R LE QLR+K R++ Q++++ E EL RV EL++ ++KL +N +K++ L+ DT E+ + R E+ +S+ Q L +I+Q+ D D E TG + + + +P F ++ S I ++S + + ++ N ++ D E E L +++ A RD +E ++ +K+ EA R +N++ + + E+ +E+ ++ + E L E ERLK+ N EL R REALE+++E+ ++ +R KE ER QR + LE+K+S K+D+V +E++++ LE++VL EK+ + ALS+ E S+AELE+ +N+ + L ++++K+ + E L + +R I QLE ER++L ++R E + LK++ E E L ++ +R + E +R EE+L +++E+ L +Q+ L ++L + RRE++ +++ ++ E LT + EL VQ+ A E E ++ E A L + +E LE+TLY+ Q+ QLE R+EQLEGE L + K++L E RQ + E E + R+Q + + Q ++ ++++ + H + V+RL + ES R ++++ + R E++D++ + E KQ+++ EI+ +Q ER++SLL E+EKQ++L L E EK +++EKL+ TQ EL + E++R ++E+ +QEQDK ++ + SELK+ +S FED H E K+L +K L+ ++ KEV +L T L++AE++RD+LRR+I+E+ R L+E EE E ++E EL+R + + RE+E + SN LR IK +E+ER L + + +Q+++V E +++E LR L+DVE++ ++ +R+LQELRR +K L E + +EV +LQ R+ ++E+KE ++RRE+ L+Q++ E+E+ REA R+++ Q+H+ LE+ + ++++ + SR ++L +A SLQ+ +++ + R+ L E RV ALE+ +++ + +++E E + ++S+LRR IG R + R+ ++ TKG D+++ + +G+ +P R SP SRSP P ++ PE V+ ALR+F+Q+L D +R+RD+A Q SL +L++ E +++ Q+LQ+ L D +E ++G + RL +A T + + ++ +R +E +R+A E++ + + E E R +DRL++ R+ + R E E K ++ +E +ENR T+L+L KRSLEG+L+R +M+L ++E E + L +R + L +Q++D E + SLQ + R+++AL++ ++ ++K Q L +L + LQ +S+LQ AL ++EQDRR+L+ERL+S + AL ++K N L + IQ Q L + EL+ + ++++Q+ E+E + +QE++ +Q + A E +KQEI + LEK + L+ +L KV+R++L+ E A + E+ +L + L + E QRQ LQ Q+ E E++H LME++ RHRQE+ E + Q+E TLE RER+HRQRV+GLEEQI++L++QL E++RR
Sbjct: 126 GETEAVLDLRQRFHEEEMAYKRKLSAYQEGQQRQAQL--------------VQRLQDKLQ-ARLDSAETKLNRMDQN----------HSLELNNALTRLDEEQQRSSSLAQVNNTLREQMEQLKTTNEKLTEEIKRLTSDLHKSHEDLDQREAEWRKERQNYTFYLSNEHSGILTLWRKAAALRSQFLDMRGITERGLAEIKTDISRSSRRMHTACLNLDSNLRLSQSAAGASLHTAR-------LEQQLRDKVRQMMQLQSQWDAEKVELNSRVSELSLQTEKLVRENSKKEKEKQILEN-SGCDTLQEL----EGVR---EECDSLQQVLSSITQMCQTDVSPDPTCVESTGVSATNEMRQ----------------SVSPLFVFSS-----SPIRRRSPTRMRSPQRSELALKAVQNVLQRHQDREKE---LHSELAAARDSLEQTKKQQEKNQHVSEAHTEELKKENKEINTILESVKQELQRCQNSIELLTREKEDMEESLQTIKQKAEGSLLETERLKAVNAELLRQREALEEQKEELNRERDRSQKELERGQRIQEQLEEKVSILKKDLVTVRESLSQTALERDVLQGEKEAVASALSKAESSRAELELALNQMHSERATLTDSLAKMGALNEGLAQDKVALNRVILQLEGERAALHEDRRAGEANCAALKEQQEALEHEVSQLQADRVGLERRCREAEEKHKRQEEQLLGVHKERGKLQDQLAQANTLVQTLQDQLKESRREMDLQGSALQRAAQRMEELTSQNAELGVQLAALEDERQEHEEEFAQLRAQKESLESTLYETQRRAAQLEDRREQLEGEIHTLTLVKQSLQVETQ--RQALEAE-EALAGLRQQKVTVELQLAQTEQAAQLSLSNQSQQHQDTVDRLKREKESLRLTMIADKQDSVQRLEKEKEDLLFDRENVKQKLSAEILRLQEEREESLLKVESEKQKALLLKETEKNSLSEKLMNTQRELSDTKMEMERCRREAQIKQEQDKTSLDNVLSELKALQSDFEDAVSAHSKENKNLRDRMKQLSQERESLNKEVEELRTQLRLAEDSRDSLRRDIIEVQRLLREKEEICESQRKETLELRRDVGDLTRERETLQKSNTELRACIKKIESERSSLQVAMEEKQQRISVLQEGKSCAEREAAHLRATLRDVERSHIEARRQLQELRRQVKTLGGESSQKEQEVAELQARIQQEEQKEQQSRRESLELRQRITESESEREAARREILTLQQHLSALESSSRQREKELEQRLSESRAGEQRLQEACKSLQEQLQRCTSESGDSRLQLGAFEARVRALEAELSKSQASRREAESHVNGLYSALRRTIGVRGRSPSPRRALSS--------------------TKGLDSSHGSPMEGRVTPSSRGVSPDR-----SRSP-----YP-ELEPELVKQALRDFMQELRDTQRERDEAGIQVLSLRRQLEDSINSCERLQSQQQKLQRQLADLQEGQRGTEERLGTAHTEMRLLQDNLRHSEAERQASGERIMELEHSLRICEDENRDFQDRLSRARNAENRQELECKGLREVLEASENRGTELELRKRSLEGELERTRMSLAEREAEVQTLQERAHHLQEQLKDSEDREASLQTEVQRLNLALSRAQDGERQLQEKSQGLTQALGEATAGRGNLQEDVSRLQGALTAAEQDRRVLQERLDSVRQALSDSKRHNLRLSQTIQEQQQQLEEMHLNCRELQAQLQDLQQTLIKQQQREEEQGAALLE-------VQEKLKMLQGDKAQVELDKQEIQHSAVLLEKGNNTLRATLDKVKREQLRIEGEAQRLSVEKEQLSHSVISLRKDLAEAQRQNQYLQEQMTEMERSHTQRLMEVSLRHRQELDSESNRAHSIQQQVESTLESRERSHRQRVRGLEEQINSLQEQLEQEVRRR 1943
BLAST of Rootletin vs. Ensembl Xenopus
Match: ENSXETT00000012563.1 (centrosomal protein 250 [Source:NCBI gene;Acc:100498015]) HSP 1 Score: 87.4261 bits (215), Expect = 2.894e-16 Identity = 45/133 (33.83%), Postives = 78/133 (58.65%), Query Frame = 3
Query: 426 YDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNL 824
+ +LE L KLEEEQ++ L N L+E L ++N+ L D+ KL+ +W + +EL+ +E W E +N + EHS L + + FRR F ELK++TER++++++ +++R +Q+ CS+L
Sbjct: 40 HGETELERALQKLEEEQQRCENLAAVNALLREDLGHTQEANKVLCNDMHKLTADWTSALQELKHKENDWEQEREHYNSLMKGEHSRLLRIWTEVVTFRRHFRELKSATERDLSSVRADWSRCLCLIQSCCSSL 172
BLAST of Rootletin vs. Ensembl Mouse
Match: Crocc (ciliary rootlet coiled-coil, rootletin [Source:MGI Symbol;Acc:MGI:3529431]) HSP 1 Score: 919.457 bits (2375), Expect = 0.000e+0 Identity = 692/1936 (35.74%), Postives = 1136/1936 (58.68%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR--------------FLEQ-------EKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSN---CDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
+GL++ ++ELR++L+EE + YRRKL +Q+ QQ+QAQLVQRLQ K+LQYK++C+ELE Q D T + R + + DL+S L++LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W RS +ELEQRE +WR EE SFN Y +SEHS L L R + RRQ E+K TER++ L E R +R +Q LS ++ AESK + + E++ + LE QL+ K ++L Q + + + +L RV EL + + L+ QN EKD+ L AL+ ++ ++++ T + ED E + Q LR+++Q L+D++ D D PR + + R +P +D+ T + SA++K+ LQ+Q+++ + +S+E + ++Q+ D+E ERR LE Q+ +RD+ + + ++D+ REA R ++A + S E+ L Q ++ E+E+L++A EL+R LED QED+ ++ R +E ER R ++ LE K S +++V +EA++ L+++VL EK E+ +AL++ E +A+LE+ + K + E +L +++SK+S+ E L + E +R I QLE E+ +LL ++ LEQ E+ GL+ L +EQ L + + R E+ L EQ+ L RQ S +EL Q RE +R ++ ++EKE + KE+ L V++ A ERE R SE L ++E LE++L+D Q+ + QLE R+EQLE ++Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL E+ + ER +L + ER++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H VK L +L ++ ++E +L T L++ E+TRD LRRE++E RK ++++++ E ++E +EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E V+ +E ELR +L++VE++RL+ +RELQELRR +K LD++ G+L +E+ DLQ R+A E E E+RRE GL+Q++++ E+S EA++++L +QR + + E ++++R +E +R ++L D+ SL+ + + A+ +EL + LS AEGR LE +ARVE ++ E +L + S+LRR +G R +S + + + DG SP P SP S+ PS P D++PEAVR ALR+F+Q+L A+R+RD+ Q +L +L E E + ++A +R +QLQ+A+ ++EE + D RLS AQ L +QEE++RR++R+ +A ++++ +R+ + ESE R +++++K + +A+LE + ++ K+ ++ +E+R +L+L +R+LEG+LQR ++ L D+E + L DRV+SL +Q+ D E K+ +LQLT++R+S ALAK EE + K+Q L +L+ S L Q+K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ +L E +R + EG +Q + QRQE E + QKL E+ LQER+ S+QR LA EAEK+++ ++ + +KDR AL+K+L KVER+KL++ E + ER LD+ L E + E Q+Q+ L+ Q+ A + + + + + E+ E+E LR++ VQ ERTLE RERAHRQRV GLEEQ+STLK QL E++R
Sbjct: 137 RGLVRQSVELRRQLQEEQSSYRRKLQAYQEGQQRQAQLVQRLQAKILQYKKQCSELEK--QLMDRSTELEQQ----------RLRDTEHSQDLDSALLRLEEEQQRSASLAQVNAMLREQLDQANLANQALSEDIRKVTSDWTRSCKELEQREAVWRREEESFNTYFSSEHSRLLRLWRQVMGLRRQASEVKMGTERDLLQLGGELVRTSRAVQELGLGLSASLHRAESKAEAALEKQKLLQAQLEEQLQAKLLREKDLAQLQVQSDLDKADLSARVTELALSVEHLQNQNSEKDQVNRTLSDKLEALESLR---------LQEQTT-LDTEDGEGLQQTLRDLAQAALSDTESGVQLSSSERTADTSDGSLRGFSGQRTPTPPRHSPGRGRSPRRGLSPACSDSSTLTLIHSALHKRQLQVQDMRGRYEASQELLGSVRKQLSDSEGERRGLEEQLQRLRDQTAASAQAQEDAQREAQRLRSANELLSREKGNLTHSLQVTQQQAKELRQELEKLQAAQEELKRQHNQLEDAQEDSVQEGARARRELERSHRQLEQLEVKRSGLTKELVEVREALSCAILQRDVLQTEKAEVAEALTKAEAGRAQLELSLTKLRAEEASLRDSLSKMSALNESLAQDKLELNRLIAQLEEEKVALLGRQQQAEHATTMAVEKQELLEQLRLEQEVERQGLQGSLCVAEQAREALEQQILVLR---------------------SERSHLQEQLAQLSRQLSGRDQELEQALRESQRQVEALERAAREKEAMAKERAGLAVKLAAAEREGRTLSEEAIRLRLEKEALESSLFDVQRQLAQLEARREQLEADSQALLLAKETLTGELAGLRQQVTSTEEKAALDKELMTQKLVQAEREAQASLREQRAAHEEDLQRLQHEKEAAWRELQAERAQLQGQLQQEREELLARMEAEKEELSKEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLAAISLEMERQKRDAQSRQEQDRNTLNALTSELRDLRAQLEEATAAHAQTVKELEERTGNLGRQREACMREAEELRTQLRVLEDTRDGLRRELLEAQRKGRDSQDSSEAHRQEASELRRSLSEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARVSVAKEAGELRASLQEVERSRLEARRELQELRRQMKTLDSDNGRLGRELADLQGRLALGERTEKESRREALGLRQRLLKGESSLEALKQELQGSQRKLQEQEAEFRARERGLLGSLEEARGAEKRLLDSARSLELRLEAVRAETSELGLRLSAAEGRAQGLEVELARVEAQRRVAEAQLGGLRSALRRGLGL---------GRVSS-------------------SPAREAPAGGSGDGLSSPSPLEYSPRSQ------PPSPGLIASPAPPDLDPEAVRDALRDFLQELRSAQRERDELKVQTSTLSQQLVEMEAERDHAASRAKQLQKAVAESEEAWRSADRRLSGAQAELALQEESVRRSKRECRATLDQMAVLERSLQATESELRASQEKVSKMKATEAKLESDKRRLKEVLDASESRSIKLELQRRALEGELQRSRLGLGDREAHAQALQDRVDSLQRQVADSEVKAGTLQLTVERLSGALAKVEESEGNLRSKVQSLTDALTQSSASLSSTQDKNLHLQKALSTCEHDRQVLQERLDAARQALSEARRQSSSLGEQVQTLRGELASLELQRGDAEGQLQQLQQALRQRQEGEAMALRSVQKLQEERRLLQERLGSLQRALAQLEAEKRDLERSALQFDKDRVALRKTLDKVEREKLRSHEDTLRLNAERGRLDRTLTGAELDLAEAQQQIQHLEAQVDVALEGNHNPVQPEAGEQQLELQQEVERLRSAQVQTERTLEARERAHRQRVSGLEEQVSTLKAQLHQELRR 1995
BLAST of Rootletin vs. Ensembl Mouse
Match: Crocc (ciliary rootlet coiled-coil, rootletin [Source:MGI Symbol;Acc:MGI:3529431]) HSP 1 Score: 894.419 bits (2310), Expect = 0.000e+0 Identity = 654/1845 (35.45%), Postives = 1079/1845 (58.48%), Query Frame = 3
Query: 438 DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR--------------FLEQ-------EKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSN---CDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
DL+S L++LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W RS +ELEQRE +WR EE SFN Y +SEHS L L R + RRQ E+K TER++ L E R +R +Q LS ++ AESK + + E++ + LE QL+ K ++L Q + + + +L RV EL + + L+ QN EKD+ L AL+ ++ ++++ T + ED E + Q LR+++Q L+D++ D D PR + + R +P +D+ T + SA++K+ LQ+Q+++ + +S+E + ++Q+ D+E ERR LE Q+ +RD+ + + ++D+ REA R ++A + S E+ L Q ++ E+E+L++A EL+R LED QED+ ++ R +E ER R ++ LE K S +++V +EA++ L+++VL EK E+ +AL++ E +A+LE+ + K + E +L +++SK+S+ E L + E +R I QLE E+ +LL ++ LEQ E+ GL+ L +EQ L + + R E+ L EQ+ L RQ S +EL Q RE +R ++ ++EKE + KE+ L V++ A ERE R SE L ++E LE++L+D Q+ + QLE R+EQLE ++Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL E+ + ER +L + ER++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H VK L +L ++ ++E +L T L++ E+TRD LRRE++E RK ++++++ E ++E +EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E V+ +E ELR +L++VE++RL+ +RELQELRR +K LD++ G+L +E+ DLQ R+A E E E+RRE GL+Q++++ E+S EA++++L +QR + + E ++++R +E +R ++L D+ SL+ + + A+ +EL + LS AEGR LE +ARVE ++ E +L + S+LRR +G R +S + + + DG SP P SP S+ PS P D++PEAVR ALR+F+Q+L A+R+RD+ Q +L +L E E + ++A +R +QLQ+A+ ++EE + D RLS AQ L +QEE++RR++R+ +A ++++ +R+ + ESE R +++++K + +A+LE + ++ K+ ++ +E+R +L+L +R+LEG+LQR ++ L D+E + L DRV+SL +Q+ D E K+ +LQLT++R+S ALAK EE + K+Q L +L+ S L Q+K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ +L E +R + EG +Q + QRQE E + QKL E+ LQER+ S+QR LA EAEK+++ ++ + +KDR AL+K+L KVER+KL++ E + ER LD+ L E + E Q+Q+ L+ Q+ A + + + + + E+ E+E LR++ VQ ERTLE RERAHRQRV GLEEQ+STLK QL E++R
Sbjct: 52 DLDSALLRLEEEQQRSASLAQVNAMLREQLDQANLANQALSEDIRKVTSDWTRSCKELEQREAVWRREEESFNTYFSSEHSRLLRLWRQVMGLRRQASEVKMGTERDLLQLGGELVRTSRAVQELGLGLSASLHRAESKAEAALEKQKLLQAQLEEQLQAKLLREKDLAQLQVQSDLDKADLSARVTELALSVEHLQNQNSEKDQVNRTLSDKLEALESLR---------LQEQTT-LDTEDGEGLQQTLRDLAQAALSDTESGVQLSSSERTADTSDGSLRGFSGQRTPTPPRHSPGRGRSPRRGLSPACSDSSTLTLIHSALHKRQLQVQDMRGRYEASQELLGSVRKQLSDSEGERRGLEEQLQRLRDQTAASAQAQEDAQREAQRLRSANELLSREKGNLTHSLQVTQQQAKELRQELEKLQAAQEELKRQHNQLEDAQEDSVQEGARARRELERSHRQLEQLEVKRSGLTKELVEVREALSCAILQRDVLQTEKAEVAEALTKAEAGRAQLELSLTKLRAEEASLRDSLSKMSALNESLAQDKLELNRLIAQLEEEKVALLGRQQQAEHATTMAVEKQELLEQLRLEQEVERQGLQGSLCVAEQAREALEQQILVLR---------------------SERSHLQEQLAQLSRQLSGRDQELEQALRESQRQVEALERAAREKEAMAKERAGLAVKLAAAEREGRTLSEEAIRLRLEKEALESSLFDVQRQLAQLEARREQLEADSQALLLAKETLTGELAGLRQQVTSTEEKAALDKELMTQKLVQAEREAQASLREQRAAHEEDLQRLQHEKEAAWRELQAERAQLQGQLQQEREELLARMEAEKEELSKEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLAAISLEMERQKRDAQSRQEQDRNTLNALTSELRDLRAQLEEATAAHAQTVKELEERTGNLGRQREACMREAEELRTQLRVLEDTRDGLRRELLEAQRKGRDSQDSSEAHRQEASELRRSLSEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARVSVAKEAGELRASLQEVERSRLEARRELQELRRQMKTLDSDNGRLGRELADLQGRLALGERTEKESRREALGLRQRLLKGESSLEALKQELQGSQRKLQEQEAEFRARERGLLGSLEEARGAEKRLLDSARSLELRLEAVRAETSELGLRLSAAEGRAQGLEVELARVEAQRRVAEAQLGGLRSALRRGLGL---------GRVSS-------------------SPAREAPAGGSGDGLSSPSPLEYSPRSQ------PPSPGLIASPAPPDLDPEAVRDALRDFLQELRSAQRERDELKVQTSTLSQQLVEMEAERDHAASRAKQLQKAVAESEEAWRSADRRLSGAQAELALQEESVRRSKRECRATLDQMAVLERSLQATESELRASQEKVSKMKATEAKLESDKRRLKEVLDASESRSIKLELQRRALEGELQRSRLGLGDREAHAQALQDRVDSLQRQVADSEVKAGTLQLTVERLSGALAKVEESEGNLRSKVQSLTDALTQSSASLSSTQDKNLHLQKALSTCEHDRQVLQERLDAARQALSEARRQSSSLGEQVQTLRGELASLELQRGDAEGQLQQLQQALRQRQEGEAMALRSVQKLQEERRLLQERLGSLQRALAQLEAEKRDLERSALQFDKDRVALRKTLDKVEREKLRSHEDTLRLNAERGRLDRTLTGAELDLAEAQQQIQHLEAQVDVALEGNHNPVQPEAGEQQLELQQEVERLRSAQVQTERTLEARERAHRQRVSGLEEQVSTLKAQLHQELRR 1831
BLAST of Rootletin vs. Ensembl Mouse
Match: Crocc (ciliary rootlet coiled-coil, rootletin [Source:MGI Symbol;Acc:MGI:3529431]) HSP 1 Score: 894.419 bits (2310), Expect = 0.000e+0 Identity = 654/1845 (35.45%), Postives = 1079/1845 (58.48%), Query Frame = 3
Query: 438 DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR--------------FLEQ-------EKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSN---CDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
DL+S L++LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W RS +ELEQRE +WR EE SFN Y +SEHS L L R + RRQ E+K TER++ L E R +R +Q LS ++ AESK + + E++ + LE QL+ K ++L Q + + + +L RV EL + + L+ QN EKD+ L AL+ ++ ++++ T + ED E + Q LR+++Q L+D++ D D PR + + R +P +D+ T + SA++K+ LQ+Q+++ + +S+E + ++Q+ D+E ERR LE Q+ +RD+ + + ++D+ REA R ++A + S E+ L Q ++ E+E+L++A EL+R LED QED+ ++ R +E ER R ++ LE K S +++V +EA++ L+++VL EK E+ +AL++ E +A+LE+ + K + E +L +++SK+S+ E L + E +R I QLE E+ +LL ++ LEQ E+ GL+ L +EQ L + + R E+ L EQ+ L RQ S +EL Q RE +R ++ ++EKE + KE+ L V++ A ERE R SE L ++E LE++L+D Q+ + QLE R+EQLE ++Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL E+ + ER +L + ER++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H VK L +L ++ ++E +L T L++ E+TRD LRRE++E RK ++++++ E ++E +EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E V+ +E ELR +L++VE++RL+ +RELQELRR +K LD++ G+L +E+ DLQ R+A E E E+RRE GL+Q++++ E+S EA++++L +QR + + E ++++R +E +R ++L D+ SL+ + + A+ +EL + LS AEGR LE +ARVE ++ E +L + S+LRR +G R +S + + + DG SP P SP S+ PS P D++PEAVR ALR+F+Q+L A+R+RD+ Q +L +L E E + ++A +R +QLQ+A+ ++EE + D RLS AQ L +QEE++RR++R+ +A ++++ +R+ + ESE R +++++K + +A+LE + ++ K+ ++ +E+R +L+L +R+LEG+LQR ++ L D+E + L DRV+SL +Q+ D E K+ +LQLT++R+S ALAK EE + K+Q L +L+ S L Q+K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ +L E +R + EG +Q + QRQE E + QKL E+ LQER+ S+QR LA EAEK+++ ++ + +KDR AL+K+L KVER+KL++ E + ER LD+ L E + E Q+Q+ L+ Q+ A + + + + + E+ E+E LR++ VQ ERTLE RERAHRQRV GLEEQ+STLK QL E++R
Sbjct: 52 DLDSALLRLEEEQQRSASLAQVNAMLREQLDQANLANQALSEDIRKVTSDWTRSCKELEQREAVWRREEESFNTYFSSEHSRLLRLWRQVMGLRRQASEVKMGTERDLLQLGGELVRTSRAVQELGLGLSASLHRAESKAEAALEKQKLLQAQLEEQLQAKLLREKDLAQLQVQSDLDKADLSARVTELALSVEHLQNQNSEKDQVNRTLSDKLEALESLR---------LQEQTT-LDTEDGEGLQQTLRDLAQAALSDTESGVQLSSSERTADTSDGSLRGFSGQRTPTPPRHSPGRGRSPRRGLSPACSDSSTLTLIHSALHKRQLQVQDMRGRYEASQELLGSVRKQLSDSEGERRGLEEQLQRLRDQTAASAQAQEDAQREAQRLRSANELLSREKGNLTHSLQVTQQQAKELRQELEKLQAAQEELKRQHNQLEDAQEDSVQEGARARRELERSHRQLEQLEVKRSGLTKELVEVREALSCAILQRDVLQTEKAEVAEALTKAEAGRAQLELSLTKLRAEEASLRDSLSKMSALNESLAQDKLELNRLIAQLEEEKVALLGRQQQAEHATTMAVEKQELLEQLRLEQEVERQGLQGSLCVAEQAREALEQQILVLR---------------------SERSHLQEQLAQLSRQLSGRDQELEQALRESQRQVEALERAAREKEAMAKERAGLAVKLAAAEREGRTLSEEAIRLRLEKEALESSLFDVQRQLAQLEARREQLEADSQALLLAKETLTGELAGLRQQVTSTEEKAALDKELMTQKLVQAEREAQASLREQRAAHEEDLQRLQHEKEAAWRELQAERAQLQGQLQQEREELLARMEAEKEELSKEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLAAISLEMERQKRDAQSRQEQDRNTLNALTSELRDLRAQLEEATAAHAQTVKELEERTGNLGRQREACMREAEELRTQLRVLEDTRDGLRRELLEAQRKGRDSQDSSEAHRQEASELRRSLSEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARVSVAKEAGELRASLQEVERSRLEARRELQELRRQMKTLDSDNGRLGRELADLQGRLALGERTEKESRREALGLRQRLLKGESSLEALKQELQGSQRKLQEQEAEFRARERGLLGSLEEARGAEKRLLDSARSLELRLEAVRAETSELGLRLSAAEGRAQGLEVELARVEAQRRVAEAQLGGLRSALRRGLGL---------GRVSS-------------------SPAREAPAGGSGDGLSSPSPLEYSPRSQ------PPSPGLIASPAPPDLDPEAVRDALRDFLQELRSAQRERDELKVQTSTLSQQLVEMEAERDHAASRAKQLQKAVAESEEAWRSADRRLSGAQAELALQEESVRRSKRECRATLDQMAVLERSLQATESELRASQEKVSKMKATEAKLESDKRRLKEVLDASESRSIKLELQRRALEGELQRSRLGLGDREAHAQALQDRVDSLQRQVADSEVKAGTLQLTVERLSGALAKVEESEGNLRSKVQSLTDALTQSSASLSSTQDKNLHLQKALSTCEHDRQVLQERLDAARQALSEARRQSSSLGEQVQTLRGELASLELQRGDAEGQLQQLQQALRQRQEGEAMALRSVQKLQEERRLLQERLGSLQRALAQLEAEKRDLERSALQFDKDRVALRKTLDKVEREKLRSHEDTLRLNAERGRLDRTLTGAELDLAEAQQQIQHLEAQVDVALEGNHNPVQPEAGEQQLELQQEVERLRSAQVQTERTLEARERAHRQRVSGLEEQVSTLKAQLHQELRR 1831
BLAST of Rootletin vs. Ensembl Mouse
Match: Crocc (ciliary rootlet coiled-coil, rootletin [Source:MGI Symbol;Acc:MGI:3529431]) HSP 1 Score: 894.419 bits (2310), Expect = 0.000e+0 Identity = 654/1845 (35.45%), Postives = 1079/1845 (58.48%), Query Frame = 3
Query: 438 DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR--------------FLEQ-------EKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSN---CDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
DL+S L++LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W RS +ELEQRE +WR EE SFN Y +SEHS L L R + RRQ E+K TER++ L E R +R +Q LS ++ AESK + + E++ + LE QL+ K ++L Q + + + +L RV EL + + L+ QN EKD+ L AL+ ++ ++++ T + ED E + Q LR+++Q L+D++ D D PR + + R +P +D+ T + SA++K+ LQ+Q+++ + +S+E + ++Q+ D+E ERR LE Q+ +RD+ + + ++D+ REA R ++A + S E+ L Q ++ E+E+L++A EL+R LED QED+ ++ R +E ER R ++ LE K S +++V +EA++ L+++VL EK E+ +AL++ E +A+LE+ + K + E +L +++SK+S+ E L + E +R I QLE E+ +LL ++ LEQ E+ GL+ L +EQ L + + R E+ L EQ+ L RQ S +EL Q RE +R ++ ++EKE + KE+ L V++ A ERE R SE L ++E LE++L+D Q+ + QLE R+EQLE ++Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL E+ + ER +L + ER++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H VK L +L ++ ++E +L T L++ E+TRD LRRE++E RK ++++++ E ++E +EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E V+ +E ELR +L++VE++RL+ +RELQELRR +K LD++ G+L +E+ DLQ R+A E E E+RRE GL+Q++++ E+S EA++++L +QR + + E ++++R +E +R ++L D+ SL+ + + A+ +EL + LS AEGR LE +ARVE ++ E +L + S+LRR +G R +S + + + DG SP P SP S+ PS P D++PEAVR ALR+F+Q+L A+R+RD+ Q +L +L E E + ++A +R +QLQ+A+ ++EE + D RLS AQ L +QEE++RR++R+ +A ++++ +R+ + ESE R +++++K + +A+LE + ++ K+ ++ +E+R +L+L +R+LEG+LQR ++ L D+E + L DRV+SL +Q+ D E K+ +LQLT++R+S ALAK EE + K+Q L +L+ S L Q+K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ +L E +R + EG +Q + QRQE E + QKL E+ LQER+ S+QR LA EAEK+++ ++ + +KDR AL+K+L KVER+KL++ E + ER LD+ L E + E Q+Q+ L+ Q+ A + + + + + E+ E+E LR++ VQ ERTLE RERAHRQRV GLEEQ+STLK QL E++R
Sbjct: 52 DLDSALLRLEEEQQRSASLAQVNAMLREQLDQANLANQALSEDIRKVTSDWTRSCKELEQREAVWRREEESFNTYFSSEHSRLLRLWRQVMGLRRQASEVKMGTERDLLQLGGELVRTSRAVQELGLGLSASLHRAESKAEAALEKQKLLQAQLEEQLQAKLLREKDLAQLQVQSDLDKADLSARVTELALSVEHLQNQNSEKDQVNRTLSDKLEALESLR---------LQEQTT-LDTEDGEGLQQTLRDLAQAALSDTESGVQLSSSERTADTSDGSLRGFSGQRTPTPPRHSPGRGRSPRRGLSPACSDSSTLTLIHSALHKRQLQVQDMRGRYEASQELLGSVRKQLSDSEGERRGLEEQLQRLRDQTAASAQAQEDAQREAQRLRSANELLSREKGNLTHSLQVTQQQAKELRQELEKLQAAQEELKRQHNQLEDAQEDSVQEGARARRELERSHRQLEQLEVKRSGLTKELVEVREALSCAILQRDVLQTEKAEVAEALTKAEAGRAQLELSLTKLRAEEASLRDSLSKMSALNESLAQDKLELNRLIAQLEEEKVALLGRQQQAEHATTMAVEKQELLEQLRLEQEVERQGLQGSLCVAEQAREALEQQILVLR---------------------SERSHLQEQLAQLSRQLSGRDQELEQALRESQRQVEALERAAREKEAMAKERAGLAVKLAAAEREGRTLSEEAIRLRLEKEALESSLFDVQRQLAQLEARREQLEADSQALLLAKETLTGELAGLRQQVTSTEEKAALDKELMTQKLVQAEREAQASLREQRAAHEEDLQRLQHEKEAAWRELQAERAQLQGQLQQEREELLARMEAEKEELSKEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLAAISLEMERQKRDAQSRQEQDRNTLNALTSELRDLRAQLEEATAAHAQTVKELEERTGNLGRQREACMREAEELRTQLRVLEDTRDGLRRELLEAQRKGRDSQDSSEAHRQEASELRRSLSEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARVSVAKEAGELRASLQEVERSRLEARRELQELRRQMKTLDSDNGRLGRELADLQGRLALGERTEKESRREALGLRQRLLKGESSLEALKQELQGSQRKLQEQEAEFRARERGLLGSLEEARGAEKRLLDSARSLELRLEAVRAETSELGLRLSAAEGRAQGLEVELARVEAQRRVAEAQLGGLRSALRRGLGL---------GRVSS-------------------SPAREAPAGGSGDGLSSPSPLEYSPRSQ------PPSPGLIASPAPPDLDPEAVRDALRDFLQELRSAQRERDELKVQTSTLSQQLVEMEAERDHAASRAKQLQKAVAESEEAWRSADRRLSGAQAELALQEESVRRSKRECRATLDQMAVLERSLQATESELRASQEKVSKMKATEAKLESDKRRLKEVLDASESRSIKLELQRRALEGELQRSRLGLGDREAHAQALQDRVDSLQRQVADSEVKAGTLQLTVERLSGALAKVEESEGNLRSKVQSLTDALTQSSASLSSTQDKNLHLQKALSTCEHDRQVLQERLDAARQALSEARRQSSSLGEQVQTLRGELASLELQRGDAEGQLQQLQQALRQRQEGEAMALRSVQKLQEERRLLQERLGSLQRALAQLEAEKRDLERSALQFDKDRVALRKTLDKVEREKLRSHEDTLRLNAERGRLDRTLTGAELDLAEAQQQIQHLEAQVDVALEGNHNPVQPEAGEQQLELQQEVERLRSAQVQTERTLEARERAHRQRVSGLEEQVSTLKAQLHQELRR 1831
BLAST of Rootletin vs. Ensembl Mouse
Match: Crocc2 (ciliary rootlet coiled-coil, rootletin family member 2 [Source:MGI Symbol;Acc:MGI:3045962]) HSP 1 Score: 313.153 bits (801), Expect = 1.559e-85 Identity = 386/1421 (27.16%), Postives = 742/1421 (52.22%), Query Frame = 3
Query: 432 NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTAL----QRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD-AEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKT--PPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELN-DKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGL-SNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDF-SSMVESSR----------RDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVD--INPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMA 4628
++DLE L +LE Q+++ L++ N L++QL ++N L R++++ + S L+++ ++ ++ +S + ++ L R + EL+ASTER + +++ + R A+ L AC NL +++ L+ + D LE +LRE+ RE+ Q ++ E LQ R+ E T+L +KL +Q + +RA+ L QR+K+ + G++ +++ L ++ ES+H L +I +V +D+ + A + E R SP R+ S A+T P AVQ+AI ++ + QEL+ +L SS+E + Q+ E R + + E E L + +EA + ++++ E+ LE L + + + E ++L+ N EL+R +++ + + ER ++E E Q ++ LE+K+S ++++ +EA++ QL++++L EK+ + AL++ E A+LE+ V + + + +++++K+++ +E L+ DK NH + QLE ER L +++ L+QE++G++++L ++ Q+ LGL E+ ++ LE + LE+++ L +E L EQ+G + +K AL ++L Q ++ E + + EK L++E+ +L+ + A ER + ++ A L ++R LE +L++AQ+L QL+T++EQLEG+ + + + L E+ RL+ ++++ K+ QL + Q ++E ++A++++ +H ED+ RL + + S E +E R+ ++ +++ +++ + EI +++ERD+SLL E+E QQ+LSL + EK +++ EL EL+R ++E+ N+Q Q + + ++ EL++ + QFE+ H+ E +L ++++ + +E +L L +A E LR+E+ + + +R L +E +EK+ + +SN LR I E E+ L R + +QK+ + E V +++E ELR L+++E+A+ D +R+LQE R ++ L+ E + +EV+DLQ +V+RD + + +E+ L+++V E +A+ + V+K++ ++ + ++E +++ + + ES R R RKL +A N AD+ L+ +L A RV LE +A+ EGA+ E +L + S+L +G + N++ R +S T G T + SP R+ SP +R PS +P I+ VR ALR+ VQ L++A+++RD++ Q +L S+L E E + ++R++QLQ+ L DAEE ++ + L SAQ A +Q+E ++R E + A A
Sbjct: 146 SMDLEEALSRLEASQQRSMGLSQVNTLLRQQLEHMQKANDTLARELTRATH----SLVHLQRKLELQESQRLSLR-----QPRDILPLWRQAKALQTHLAELRASTERGLTDVQADMTRTAQRLHMACLNLDSHLR-------LTASSMTSD---LEQRLREQAREMLQLQGQWAAEKVALQARLSEQTLLVEKLSVQKEQGERAILTLKSDIQRLKSRRSGGQLAVDE-----LRDEVESLHHVLASIKEVAQSDAMCPELAWSSSIEVREAQARLRSPPRSVSPHQRMSPARTSSPTSLHPALQAVQAAIERRQQREQELRLRLESSQEEAAGLREQLSGYRQELRTSQRLLQDRAQEHEDLLGQLEAQRQEAQLSQASVHLLEREKEALETTMEELRAKADIREAETQKLEVTNAELRRSLLLRAEQKAELAQQSERSLRELEASQGRVEQLEEKVSGLRKELATSREALSSMQLQRDILETEKESLHGALAQAESGNADLELLVTRLKAEGMEQQDSLAKMAALLEGLSQDKGTLNHLAL-QLEQERDQLREQQKMLQQEQAGMREQLTQTGQQ-LGLIRAERRSLKETCGHLEQKQDHLEKQVVLLGQENAQLREQVGQVTNKKQALEKQLAQSLQDQEAQMDILQEALHEKNTLSEERAQLLAKQEALERHSELVTKEAADLRAERNSLENSLFEAQRLTTQLQTQQEQLEGKAEAAQLARRALQVEIERLKSDWEVRETKLQLHLGQLQQQAAQQEQEAQLALERQELAHTEDLARLHR-EKDTLSLSLAEEKEAAARWMEQQKELLTRSAADREALQGEIQNLKQERDESLLQLEHEMQQALSLKDAEKSLLSK-------ELSGAHRELERARQEAQNQQVQAEATVTTMTKELRTLQVQFEEAISTHQREADTLREKLREIAAERSSVRREAEELQAQLNVAHERLAELRQELQASEESREGLQREALGA-------RRALEDEVQEKDVLQHSNTELRASIHRAEQEKASLKRSKEEQEQKLLLLQEAQVAAQKEAYELRTRLQELERAQRDTRRKLQERHRQVRTLEAENQRKRQEVSDLQAQVSRDAQHRQKNLQESLELQRQVAEAQAAHDGVQKEVLGLRQKLAEVEASGETRAKQLEGHLCESQRAEQTLQAELCRITRKLQEASNQ---------ADS--LQRSLDNACSRVHVLEQELAKAEGARCNAEAQLGRLWSTLCSGLG-QSRNLLASPKRPHSP------------------TTGSSQTRPGRQ--RTSPPTRSYSP-------ARWPSPVPVDPKSEVIDVAFVRDALRDLVQGLLEAQQERDNSGIQVANLSSQLSEAERERLRLQSRVEQLQRDLADAEEGQRRAESALQSAQAARALQKEALQRLETEHLASA 1486
BLAST of Rootletin vs. UniProt/SwissProt
Match: sp|Q8CJ40|CROCC_MOUSE (Rootletin OS=Mus musculus OX=10090 GN=Crocc PE=1 SV=2) HSP 1 Score: 919.457 bits (2375), Expect = 0.000e+0 Identity = 692/1936 (35.74%), Postives = 1136/1936 (58.68%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR--------------FLEQ-------EKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSN---CDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
+GL++ ++ELR++L+EE + YRRKL +Q+ QQ+QAQLVQRLQ K+LQYK++C+ELE Q D T + R + + DL+S L++LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W RS +ELEQRE +WR EE SFN Y +SEHS L L R + RRQ E+K TER++ L E R +R +Q LS ++ AESK + + E++ + LE QL+ K ++L Q + + + +L RV EL + + L+ QN EKD+ L AL+ ++ ++++ T + ED E + Q LR+++Q L+D++ D D PR + + R +P +D+ T + SA++K+ LQ+Q+++ + +S+E + ++Q+ D+E ERR LE Q+ +RD+ + + ++D+ REA R ++A + S E+ L Q ++ E+E+L++A EL+R LED QED+ ++ R +E ER R ++ LE K S +++V +EA++ L+++VL EK E+ +AL++ E +A+LE+ + K + E +L +++SK+S+ E L + E +R I QLE E+ +LL ++ LEQ E+ GL+ L +EQ L + + R E+ L EQ+ L RQ S +EL Q RE +R ++ ++EKE + KE+ L V++ A ERE R SE L ++E LE++L+D Q+ + QLE R+EQLE ++Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL E+ + ER +L + ER++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H VK L +L ++ ++E +L T L++ E+TRD LRRE++E RK ++++++ E ++E +EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E V+ +E ELR +L++VE++RL+ +RELQELRR +K LD++ G+L +E+ DLQ R+A E E E+RRE GL+Q++++ E+S EA++++L +QR + + E ++++R +E +R ++L D+ SL+ + + A+ +EL + LS AEGR LE +ARVE ++ E +L + S+LRR +G R +S + + + DG SP P SP S+ PS P D++PEAVR ALR+F+Q+L A+R+RD+ Q +L +L E E + ++A +R +QLQ+A+ ++EE + D RLS AQ L +QEE++RR++R+ +A ++++ +R+ + ESE R +++++K + +A+LE + ++ K+ ++ +E+R +L+L +R+LEG+LQR ++ L D+E + L DRV+SL +Q+ D E K+ +LQLT++R+S ALAK EE + K+Q L +L+ S L Q+K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ +L E +R + EG +Q + QRQE E + QKL E+ LQER+ S+QR LA EAEK+++ ++ + +KDR AL+K+L KVER+KL++ E + ER LD+ L E + E Q+Q+ L+ Q+ A + + + + + E+ E+E LR++ VQ ERTLE RERAHRQRV GLEEQ+STLK QL E++R
Sbjct: 137 RGLVRQSVELRRQLQEEQSSYRRKLQAYQEGQQRQAQLVQRLQAKILQYKKQCSELEK--QLMDRSTELEQQ----------RLRDTEHSQDLDSALLRLEEEQQRSASLAQVNAMLREQLDQANLANQALSEDIRKVTSDWTRSCKELEQREAVWRREEESFNTYFSSEHSRLLRLWRQVMGLRRQASEVKMGTERDLLQLGGELVRTSRAVQELGLGLSASLHRAESKAEAALEKQKLLQAQLEEQLQAKLLREKDLAQLQVQSDLDKADLSARVTELALSVEHLQNQNSEKDQVNRTLSDKLEALESLR---------LQEQTT-LDTEDGEGLQQTLRDLAQAALSDTESGVQLSSSERTADTSDGSLRGFSGQRTPTPPRHSPGRGRSPRRGLSPACSDSSTLTLIHSALHKRQLQVQDMRGRYEASQELLGSVRKQLSDSEGERRGLEEQLQRLRDQTAASAQAQEDAQREAQRLRSANELLSREKGNLTHSLQVTQQQAKELRQELEKLQAAQEELKRQHNQLEDAQEDSVQEGARARRELERSHRQLEQLEVKRSGLTKELVEVREALSCAILQRDVLQTEKAEVAEALTKAEAGRAQLELSLTKLRAEEASLRDSLSKMSALNESLAQDKLELNRLIAQLEEEKVALLGRQQQAEHATTMAVEKQELLEQLRLEQEVERQGLQGSLCVAEQAREALEQQILVLR---------------------SERSHLQEQLAQLSRQLSGRDQELEQALRESQRQVEALERAAREKEAMAKERAGLAVKLAAAEREGRTLSEEAIRLRLEKEALESSLFDVQRQLAQLEARREQLEADSQALLLAKETLTGELAGLRQQVTSTEEKAALDKELMTQKLVQAEREAQASLREQRAAHEEDLQRLQHEKEAAWRELQAERAQLQGQLQQEREELLARMEAEKEELSKEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLAAISLEMERQKRDAQSRQEQDRNTLNALTSELRDLRAQLEEATAAHAQTVKELEERTGNLGRQREACMREAEELRTQLRVLEDTRDGLRRELLEAQRKGRDSQDSSEAHRQEASELRRSLSEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARVSVAKEAGELRASLQEVERSRLEARRELQELRRQMKTLDSDNGRLGRELADLQGRLALGERTEKESRREALGLRQRLLKGESSLEALKQELQGSQRKLQEQEAEFRARERGLLGSLEEARGAEKRLLDSARSLELRLEAVRAETSELGLRLSAAEGRAQGLEVELARVEAQRRVAEAQLGGLRSALRRGLGL---------GRVSS-------------------SPAREAPAGGSGDGLSSPSPLEYSPRSQ------PPSPGLIASPAPPDLDPEAVRDALRDFLQELRSAQRERDELKVQTSTLSQQLVEMEAERDHAASRAKQLQKAVAESEEAWRSADRRLSGAQAELALQEESVRRSKRECRATLDQMAVLERSLQATESELRASQEKVSKMKATEAKLESDKRRLKEVLDASESRSIKLELQRRALEGELQRSRLGLGDREAHAQALQDRVDSLQRQVADSEVKAGTLQLTVERLSGALAKVEESEGNLRSKVQSLTDALTQSSASLSSTQDKNLHLQKALSTCEHDRQVLQERLDAARQALSEARRQSSSLGEQVQTLRGELASLELQRGDAEGQLQQLQQALRQRQEGEAMALRSVQKLQEERRLLQERLGSLQRALAQLEAEKRDLERSALQFDKDRVALRKTLDKVEREKLRSHEDTLRLNAERGRLDRTLTGAELDLAEAQQQIQHLEAQVDVALEGNHNPVQPEAGEQQLELQQEVERLRSAQVQTERTLEARERAHRQRVSGLEEQVSTLKAQLHQELRR 1995
BLAST of Rootletin vs. UniProt/SwissProt
Match: sp|Q5TZA2|CROCC_HUMAN (Rootletin OS=Homo sapiens OX=9606 GN=CROCC PE=1 SV=1) HSP 1 Score: 894.804 bits (2311), Expect = 0.000e+0 Identity = 706/1937 (36.45%), Postives = 1145/1937 (59.11%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKM--QFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREK---NRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRA-------LTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD----------YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXX-AKTPPFADA-TYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKS---------------------GLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINS-RHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQR 5837
+GL++ ++ELR++L+EE YRRKL +Q+ QQ+QAQLVQRLQ K+LQYK++C+ELE ++ + G+ E ++ + + + DLES LI+LEEEQ+++ L + N L+EQL QA +NQ L+ DI K++ +W R R+ELE RE WR EE SFN Y ++EHS L L R + FRR E+K TER++ L E R +R +Q A LS ++ AES+ + + E+++ + LE QLR+K ++L Q + + + +L RV EL + +L+ QNLEKD+ L AL+ ++ ++++A + ED E + Q LR+++Q VL+DS+ DA + + PRR+ S +P +D+ T A + SA++K+ LQ+Q+++ + +S++ ++Q+ D+E+ERR LE Q+ +RD+ + + +D+ RE R ++A + S E+S L Q E+ E E+L++A EL+R R+ LE+EQED +D R +E ER R ++ LE K S +++V +EA+++ L++++L EK E+ +AL++ E + ELE+ + K + E +L++++SKLS+ E L + + +R + QLE E+S+L +R EQE + GL+ L +EQ L + R E+ L EQ+ L RQ S +EL Q RRE +R ++ ++EKE L KE L VQ+ A ERE R SE L ++E LE +L++ Q+ + QLE R+EQLE E Q L++ KE L GEL LRQ+ EK +E + + Q ++E + +++++R +H ED++RL + E+ E ER +L + E+++++ E EK+E+++EI +Q+ERD+ LL+AE+EKQQ+LSL E EK A++EKL+ T+ L ++ E++R K+++ +RQEQD+ + L+SEL+ R+Q E+ H EV+ L +DL +D L+E +L T L++ E+ RD LRRE++E RKL+E++E +E ++E EL+R L E +E+E + SNE LR +K E+ERI L D +QK+A+ +E +E ELR L++VE++RL+ +RELQELRR +K LD+E +L +E+ +LQ R+A E E E+RRE GL+Q++++ EAS E +R++L AQR + + E ++++R +E +R ++ D L+ + A+ EL + LS AEGR LE+ +ARVE ++ E +L + S+LRR +G + R S + G+G P T S + S P+ +P D++PEAVR ALREF+Q+L A+R+RD+ Q +L +L E E + + A +R +QLQ+A+ ++EE ++ +DGRLS Q L +QEE++RR+ER+R+A ++++ +R+ + ESE R +++++K + ++ +LE + ++ K+ ++ +E+R +L+L +RSLEG+LQR ++ L+D+E + + L DRV+SL +Q+ D E K+ +LQLT++R++ ALAK EE +DK++ L +L+ S L ++K LQKAL + E DR++L+ERL++++ AL EA+ Q++ L E++Q ++ ++ D E +R E EG +Q ++ QRQE E QKL E+ LQER+ S+QR LA EAEK+E+ ++ +LEKDR AL+++L KVER+KL++ E + E+ LD+ L E E E QRQ+ L+ Q+ EQ+H +E+++ + + E+ E+E LR++ Q ERTLE RERAHRQRV+GLEEQ+STLK QL E++R
Sbjct: 135 RGLVRQSVELRRQLQEEQASYRRKLQAYQEGQQRQAQLVQRLQGKILQYKKRCSELEQQLLERSGELEQQRLR--------------DTEHSQDLESALIRLEEEQQRSASLAQVNAMLREQLDQAGSANQALSEDIRKVTNDWTRCRKELEHREAAWRREEESFNAYFSNEHSRLLLLWRQVVGFRRLVSEVKMFTERDLLQLGGELARTSRAVQEAGLGLSTGLRLAESRAEAALEKQALLQAQLEEQLRDKVLREKDLAQQQMQSDLDKADLSARVTELGLAVKRLEKQNLEKDQVNKDLTEKLEALESLR---------LQEQAA-LETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGSLRGLSGQRTPSPPRRSSPGRGRSPRRGPSPACSDSSTLALIHSALHKRQLQVQDMRGRYEASQDLLGTLRKQLSDSESERRALEEQLQRLRDKTDGAMQAHEDAQREVQRLRSANELLSREKSNLAHSLQVAQQQAEELRQEREKLQAAQEELRRQRDRLEEEQEDAVQDGARVRRELERSHRQLEQLEGKRSVLAKELVEVREALSRATLQRDMLQAEKAEVAEALTKAEAGRVELELSMTKLRAEEASLQDSLSKLSALNESLAQDKLDLNRLVAQLEEEKSALQGRQRQAEQEATVAREEQERLEELRLEQEVARQGLEGSLRVAEQAQEALEQQLPTLRH---------------------ERSQLQEQLAQLSRQLSGREQELEQARREAQRQVEALERAAREKEALAKEHAGLAVQLVAAEREGRTLSEEATRLRLEKEALEGSLFEVQRQLAQLEARREQLEAEGQALLLAKETLTGELAGLRQQIIATQEKASLDKELMAQKLVQAEREAQASLREQRAAHEEDLQRLQREKEAAWRELEAERAQLQSQLQREQEELLARLEAEKEELSEEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLATISLEMERQKRDAQSRQEQDRSTVNALTSELRDLRAQREEAAAAHAQEVRRLQEQARDLGKQRDSCLREAEELRTQLRLLEDARDGLRRELLEAQRKLRESQEGREVQRQEAGELRRSLGEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARTAVGKEAGELRTGLQEVERSRLEARRELQELRRQMKMLDSENTRLGRELAELQGRLALGERAEKESRRETLGLRQRLLKGEASLEVMRQELQVAQRKLQEQEGEFRTRERRLLGSLEEARGTEKQQLDHARGLELKLEAARAEAAELGLRLSAAEGRAQGLEAELARVEVQRRAAEAQLGGLRSALRRGLGLGRAPSPAPRPVPGSPA----------------------RDAPAEGSGEGLNSPSTLECSPGSQPPSPGPATSPASP-DLDPEAVRGALREFLQELRSAQRERDELRTQTSALNRQLAEMEAERDSATSRARQLQKAVAESEEARRSVDGRLSGVQAELALQEESVRRSERERRATLDQVATLERSLQATESELRASQEKISKMKANETKLEGDKRRLKEVLDASESRTVKLELQRRSLEGELQRSRLGLSDREAQAQALQDRVDSLQRQVADSEVKAGTLQLTVERLNGALAKVEESEGALRDKVRGLTEALAQSSASLNSTRDKNLHLQKALTACEHDRQVLQERLDAARQALSEARKQSSSLGEQVQTLRGEVADLELQRVEAEGQLQQLREVLRQRQEGEAAALNTVQKLQDERRLLQERLGSLQRALAQLEAEKREVERSALRLEKDRVALRRTLDKVEREKLRSHEDTVRLSAEKGRLDRTLTGAELELAEAQRQIQQLEAQVVVLEQSHSPAQLEVDAQQQQLELQQEVERLRSAQAQTERTLEARERAHRQRVRGLEEQVSTLKGQLQQELRR 2003
BLAST of Rootletin vs. UniProt/SwissProt
Match: sp|H7BZ55|CRCC2_HUMAN (Putative ciliary rootlet coiled-coil protein 2 OS=Homo sapiens OX=9606 GN=CROCC2 PE=5 SV=4) HSP 1 Score: 271.552 bits (693), Expect = 8.533e-72 Identity = 331/1175 (28.17%), Postives = 613/1175 (52.17%), Query Frame = 3
Query: 1488 EIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEEL-NDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQE-ALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERD-DMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRH-------HDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKE-------TETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRM 4961
E E+LR + E + + + + E++ LE + L + + E + L++ ELQR + +E+ ER + E Q + LE+K+S +E++ + +EA++ QL+++V+ E++ + AL+R E S A+LE+ V + ++ V ++++ +++ +E L DK A NH + QLE ER L +++ LEQE++ ++L ++EQ+ AL + + +Q Q +LE E+LE + + L REK L EQ+G + QK AL E+L Q ++ E ++ + K+ L++E+ +L+ + A ER+ R +E A L +R+ LE++L +AQQL +L +EQLE E + + ++ L E+ +L+ +++++ K+ +L + Q ++E + A++ + +H E + +L + E+ E+E + R +E++ +++ E++ + EI ++++ERD+SLL E++ QQ+LSL E E+ ++E EL L+R+++E+ ++QEQ + I+ + ELK+ ++QFED H+ E +L ++DL + +E L L +A+E LR+E+ + E++E L RE E +R L +E REK+ ++ N LR I E E+ R + +QK+ + +E +QE + LR +L ++E+A D ++EL+EL R ++ L E + + E ++LQ + +++ L+++ + EA E RK++ QR + ++E ++ + + SR + L +S+ + + L+ L A R+ +LE +A+ EGA+++ E +L + S+LRR +G ++++ + S TKG D++ A SP R P S R S +P +D+ V+ LR+FVQ+L +A+R+RDD+ Q +L SRL E E + A++R+ QLQ+AL +AEE ++ ++G LSSA+ A +Q+E +RR E + A + + EKR+L+++L R H L ++ K ++ + N E+R Q A+ SLE L++M+ +E E E LD +NSL +++ ++Q LQ + M
Sbjct: 461 EREALRGQLEAQRLEVQQCRASCKLLGREKAALEMVVEELKGKADAADAEKQGLEAEAAELQRSLLLQAERREELALRRERSCRALETSQGRLQQLEEKVSGLREELASVREALSTAQLQRDVVESEREGLRSALARAECSNADLELLVRRLKSEGVEQRDSLAAMAALMEGLAQDKSALNHLAL-QLEQERDQLREQRKTLEQERARAGEQLAQAEQQLALERAERRGLQ-QACGRLEQRQEQLEGQAALLGREKAQLQEQVGQVTCQKQALEEQLAQSLQDQEAQMGTLQQALQGKDALSEERAQLLAKQEALERQGRLAAEEAADLRVERDSLESSLLEAQQLATKL---QEQLEEEARSAGLARQALQVEMEQLQSDWEVQEMKLRQDTVRLQRQVAQQEREAQRALESQALAHREALAQLQREKETLSLTLAEEKE--VARCQLEQEKELVTKSAAEREALKGEIQSLKQERDESLLQLEHKMQQALSLKETERSLLSE-------ELSRARRTLERVQQEAQSQQEQAQATISATTEELKALQAQFEDAITAHQRETTALRESLQDLAAERGDVEREAERLRAQLTVAQEGLAALRQELQGVE-------ESREGLHREAQEARRALSDEAREKDVLLLFNSELRATICRAEQEKASFKRSKEEKEQKLLILEEAQAALQQEASALRAHLWELEQAGGDARQELRELHRQVRTLKAENQRRSGEAHELQAQCSQEV----------LELRRQAAKAEAKHEGARKEVLGLQRKLAEVEAAGEAHGQRLQEHLRESRGAEQTLRAELHSVTRKLQEASGVADALQARLDQACHRIHSLEQELAQAEGARQDAEAQLGRLCSTLRRGLGLQRQSPWASPEQPGSP------------------TKGSDSSQALPGQQGTSPPAR---PHSPLRWPSPTPGGRSSELMDVA--TVQDILRDFVQKLREAQRERDDSRIQMATLSSRLSEAECRCARAQSRVGQLQKALAEAEEGQRRVEGALSSARAARALQKEALRRLELEHLA----------SVRAAGQEKRRLQEQLETLRQALEESRRHSQGLAKQGKLLEEQLTNLEHRC---QKAEVSLE-PLRQMEQETLKREEDVARLGAEKEQLDQSLNSLHQEVDGALRQNQQLQAQMTEM 1567 HSP 2 Score: 84.7297 bits (208), Expect = 1.037e-14 Identity = 66/209 (31.58%), Postives = 105/209 (50.24%), Query Frame = 3
Query: 432 NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTAL 1058
++DLE L +LE ++++ L + N L+EQL ++N L R+++ ++ QR + ELE R W + + +L L R + EL+ +TER + +L+ + R AR L TAC NL N++ LS S L QLR+K E+ Q R++ E LQ R+ E T+L +KL QN +K + + AL
Sbjct: 147 SVDLEEALGRLEAAEERSTGLCQVNALLREQLEHMKKANDALGRELAGMTGSVQRLQGELELRR--WAQRQTRSGGL--GQPRDLLLLWRQAVVLGTDLAELRVATERGLADLQADTARTARRLHTACLNLDSNLR-------LSA---SSTASTLGQQLRDKAGEMLQLQGRWDAEKVALQARLSEQTLLVEKLTEQNEQKAKTIAAL 341 HSP 3 Score: 62.3882 bits (150), Expect = 5.536e-8 Identity = 84/278 (30.22%), Postives = 141/278 (50.72%), Query Frame = 3
Query: 5010 KMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQ-NNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
+M L+ LS + + Q+++ QLQKAL +E+ +R +E L S++ A K L +E + +++ E RR L Q TL ++A +E + Q L + L+E++ +++ AE LE R ++E++ LK EE ++ E+ +LDQ L L E RQ LQ Q+ E EQAH L ++ ++H+++++ E E L + Q + LE +E H+Q+VK LEEQ+++LK+QL E+Q R
Sbjct: 1388 QMATLSSRLSEAECRCARAQSRVGQLQKALAEAEEGQRRVEGALSSARAARALQKEALRRLELEHLASVRA--AGQEKRR-----LQEQLETL----RQALEESRRHSQGLAKQGKLLEEQLTNLEHRCQKAEV----------SLEPLR--------QMEQETLKREEDVARLGAEKEQLDQSLNSLHQEVDGALRQNQQLQAQMTEMEQAHTQRLQDLTAQHQRDLATEAERLHGARPQATQALESQEWTHQQQVKVLEEQVASLKEQLDQEVQWR 1636
BLAST of Rootletin vs. UniProt/SwissProt
Match: sp|Q9BV73|CP250_HUMAN (Centrosome-associated protein CEP250 OS=Homo sapiens OX=9606 GN=CEP250 PE=1 SV=2) HSP 1 Score: 203.756 bits (517), Expect = 8.947e-51 Identity = 269/1011 (26.61%), Postives = 506/1011 (50.05%), Query Frame = 3
Query: 309 YKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNL----------ENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTAL-QRIKNGDTAGEINMEDKAT--RVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELN-DKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIME 3299
Y+ C ELE +++ G P + + E D L L++LEEEQ++ L E N +L+ + +A N+ L D+ KL+ +W R+R+EL ++E W+ E+ F YL EH L +L R + FRR F+E+K++T+R++ LK E R + L T C L+ +S E S + R+ L E + E+++EL +++ + E ELQ RV EL+ L + + QN + ++ + AL + ++ +T ME +A+ R E+ S+ Q +++I+QV++ + D A+ +G E + + S + + AD V+S + ++ +Q+L+ +L +E+ ++Q D E E + A+R ++ L ++D + + ++ S ER L+K R L Q+E E RL+ N ELQ ++ + ++E+ +++L ++E ER Q + LE K S +++ +EA+ LE E+L QE+ E+ AL+R E S AEL N +T +L KLS+ E L DK N + + QLE E S+ + EQ ++ L+ +L +E+ L + ++++ E L+ +L ++ EK ++ +++ + Q+ A +L Q +E +R + +EKE L +EK L V++ A ER+ + +E + L+S +E LE++L++AQQ +E K QLE + Q + KE + GE+ L+ E E + R+ + Q ++E K A++Q++ +H ++V +L + E R + E + + ++ER+ M E +E E+ IQ +R++ AE+ Q E+E+ ++ E L++TQ EL +L+R++++ ++ ++++ L ++L+ + + ++ + H ++ +L L K K+V DL + L ++++ + +E+ E
Sbjct: 66 YRSWCQELEKRLEA---------TGGPIPQRWENVEEPNLDEL-----LVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNG--SGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNI-AQGSGHENSLELDSSIFSQFDYQD------------ADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGK-------ALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLL-QLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALE--SLEREKM--ELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQE 1035
BLAST of Rootletin vs. UniProt/SwissProt
Match: sp|Q60952|CP250_MOUSE (Centrosome-associated protein CEP250 OS=Mus musculus OX=10090 GN=Cep250 PE=1 SV=4) HSP 1 Score: 194.512 bits (493), Expect = 5.156e-48 Identity = 268/1008 (26.59%), Postives = 494/1008 (49.01%), Query Frame = 3
Query: 309 YKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKL--------NLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQR---IKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIME 3299
Y+ C +LE +++ G + + +LE LI+LEEEQ++ L E N L+ + +A N+ L D+ KL+ +W R+R+EL ++E WR E+ F YL EH L NL R + FRR F+++K++T+R++ LK E R + L T C L+ +S ES TE +L LE + EK++EL ++ + E ELQ RV EL+ L + + QN + ++ + AL+ I + A + E +R E+ S+ Q ++ I+Q + + + EDT ++ + S S P D VQS + ++ +Q+L+ +L+ +E+ + ++Q D E E R A+R++++ L ++D + + ++ S ER L+K R L Q+E E RL+ N ELQ ++ + E+ + +++L ++E ER Q ++ LE K S +++ +EA+ +LE E+L QE+ E+ AL+R E S EL N + +L KL + E L + E ++ + QLE E SL + EQ +S L+ +L +E+ L +K ++++ E L+ EL EK +L +++ Q+ L Q ++ ER ++ +EKE L +E+ L V++ A ER+ + +E + L S +EQLE+ L++AQQ ++ K QLE + Q +I KE + GE+ L+ E E + + + + Q ++E + ++++++ +H E+V RL + E R + E ++ + ER ++ +++ EM E + QRE + S A++ Q E+E+ ++ E L++TQ EL +L+R++++ ++ ++++ L ++L+ + + ++ Q H ++ + DL K+V DL + L ++++ ++ EI E
Sbjct: 65 YRSWCQDLEKRLE--------------ATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKLTVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHARLSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQALAS----VEEEDTVTQSSGHEDSLQSDCNGLSQFD-------PQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFLQQQHDQWEEEGR-------ALREKLQKLTGERDALAGQTVGLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRLRRMNMELQLQGDSAQGEKLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELLKQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVELNQQLLQLEQENQSLCSRVEAAEQLRSALQVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEAHHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQLESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQEWDAVARQLAQAEQEGQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEM--EAIRAQREEERS--QADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQELKEAAQQHRDDLAAFQKDKLDLQ-------KQVEDLMSQLVAHDDSQRLVKEEIEE 1029
BLAST of Rootletin vs. TrEMBL
Match: A0A267F2Q1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001435g3 PE=4 SV=1) HSP 1 Score: 1746.87 bits (4523), Expect = 0.000e+0 Identity = 1048/2017 (51.96%), Postives = 1458/2017 (72.29%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTE--LES---KMQFGDYETRKTKYGSPTNNGYGSRSENPYDNL-DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDT------------AGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD-YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENV---VTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNT--YATTTDGKGSPIPRTGSPSSKRRQLSRS--PSNCDF-NPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFM-------CGSRDQSPTRQKRIEMSKKSAAPNN--SNRRLTAG 6107
+GL+Q+N++LRKRL++E Y+RKL +Q+ QQ+QAQLVQ+LQ KVL YK+K +E LE+ K Q ++ Y ++ RS D+ DLES LI+LEEEQ+++ L N L+EQL QATQ NQ+LT D+ +LSE++QR+R +LE+RE WR+EE SF++Y +SEH+ L +L RN ++FRRQF ELK + ER++NN KNE RQ+R + AC NL+ N+KSAE++ ++ ++E + NLE+ LRE+ REL D Q ++E +L RV EL+V ++KLK Q EKDR L+ LQR+++ A +D ATR LIE+TE+MHQ+LR+I+Q V++D+D Y D D G + R + +++P FAD+TY+AVQ+A+NK++LQ+QEL+ KL S+K++ N +RQ+D++ENERRRLE+QVL +R+E+++++R + DS R+ +R K+AL ERSQLEK+RS+LN+QIE +++RL+ AN ELQR R+ LED++ D KKDL+RQ+KE+ERCQR ID LE+K+S K D+V KE +N+ LEKEVL QEK E+ DALSR E++KAELE+E+NK ++ E L + + K+ S E L + E ++ + E+E++ L EK+ LEQEK G+++EL+R EQE + L NEKA Q +E E++ E+LE+EL +NRE+L+L EQ+ + RQK ALAEELL RRE ER++N+V ++KEKE LTKEKGELVVQ+TACERE R+ E+ + L +D++QLE L++AQ+LI+QLETRK QLEGENQELI+KKENLN E+ R+ +EF++E +K+ RE L + QQM+++ K A+ QE+ SH +D+ERLTK ++ R EN+R+E+IH+ NMER++M+ ++E+E++++ EI QRERD+SL++AEN+KQQ+LS++EQEK I E+L T ELQ+V SELDR ++E++ +Q+QDK+ I L+ ELK FR QFE + HE EV+ LN I +LT ++ A +EV +L L++AE+ RDNLRRE+++ NR+++E EEA + KREI ELKR L EE R +E V SNE LRKKIK++E ERI+ R + + KQ++ V +EQ +EV +LR NL++VEKAR++ KRELQELRR +K LDTERGKL KEV DLQ+RVA DEEK++E RRENFGLKQKVVETEASREA+RK+L N++R DLE L+SK RDF+ +E +R++ R+L DAR+ LQ + A+NNELR AL GAEGR++ALE+ +AR + KKE+E+KLASIHSS+RRLIGFRQ+N +R R+ S + + TKGFD + +ATT +G+GSPIPR+GSP+++ R RS PS D N DI+PEAVRLALR+FVQQLV+ ER+RDDA A ARSLE R+ E EE + E R QQL + LC+ EEDKKGIDGRL+SAQTALM+QEETIRRNER++KA+ +K+S +R + +E+EKRQL+++LNK + HDAR EE+ K K+++++ ENR TQL+L KR+LEGDLQRM++AL DK+ E +VL DRV++L KQI DLE K+QSLQLTI+R+SV+LAKTEE+GS KD++QQLN S+S + ++ LQ ++ QLQKAL SSE DRR+L+ERL+++ A++EAKHQN+ L+ER+Q +Q +L D+E+RR++LE NRQ+N+L+ Q+QEAEQEL QK K N E+ +QERV S+QR LA E EK+EI K+ +LEKD++ALKK+L KVER++++TEE ASK+ F R ELD LRRLE+EN LQRQV LQ+ LAEAEQAH LM++ ++HR+E E+E L+A + ERTLE RER HRQRVKGLEEQ++TLKDQL E++RR + S D+R +LDSSL V++DP LDA L + E+RRLDE++ + G R SP+R + +S+ P+ + RL+ G
Sbjct: 24 RGLMQMNMDLRKRLDDEQQSYKRKLATYQEGQQRQAQLVQKLQAKVLAYKKKVSESDLENQRLKTQLRSFKQSNDSYQFKLDSETRLRSGGDEDHSSDLESALIRLEEEQQRSASLAHVNAMLREQLDQATQVNQSLTEDMKRLSEDFQRTRSDLERRESEWRDEEASFSEYFSSEHNRLLSLWRNLVSFRRQFNELKTANERDLNNFKNEMTRQSRGVHAACQNLNANLKSAETQGAVALDQERSGRANLESALRERTRELQDLQAKFEAHQADLSGRVNELSVQNEKLKFQLAEKDRTLSHLQRLRSSGGGGGGGSASRSQSAEPPGADDAATRALIEETEAMHQSLRDIAQAVISDADGYRDFSDAG-----RGRSVSPVGRGGRSPAGRQRSRSPAFADSTYSAVQAALNKRALQVQELRGKLASAKDAGDNIRRQLDESENERRRLEHQVLGLREELDTVQRAQTDSGRDYERLKSALTAREQERSQLEKVRSALNEQIESLHADLDRLQQANSELQRCRDCLEDQKADQKKDLDRQVKENERCQRCIDQLEEKLSNLKSDLVQTKENLNRACLEKEVLDQEKAEVSDALSRSELAKAELELELNKMKSEEAALRDALLKMQSLNEGLGHDKVELNKILMMYENEKAQLSGEKQALEQEKYGIREELLRCEQEKMELDNEKAGLYQTLELSEMSREKLEDELQSINRERLELAEQLNAIGRQKRALAEELLASRRETERASNTVLRVAKEKEDLTKEKGELVVQLTACEREIRRLGELASGLRADKDQLEDALHEAQELIVQLETRKGQLEGENQELIIKKENLNAEISRITKEFEVECDKLTRQRENLQQKMQQMERDLKHALAQEQQSHEDDLERLTKEKDNQRAEYENQRQEMIHQNNMEREEMVSSFEREREQLHTEIAACQRERDESLMLAENDKQQALSIVEQEKSTILERLGNTHAELQTVASELDRARREAAAKQQQDKETINSLNGELKKFRDQFEQTVEEHEAEVRKLNEQINELTQQREAACREVAELKVQLRMAEDARDNLRRELIDANRRIREQEEALDAQKREIFELKRQLAEEGRAREAVEKSNEELRKKIKEMEAERIERDRSIAEQKQRIGVLEEQKAALGKEVADLRGNLREVEKARVEAKRELQELRRQVKMLDTERGKLCKEVCDLQSRVAHDEEKDEENRRENFGLKQKVVETEASREALRKELGNSKRREADLEEELRSKQRDFALAMEDARKELRRLDDARSELQLSVEAASAENNELRQALGGAEGRINALETTLARTDSVKKELEYKLASIHSSMRRLIGFRQDNPRSPSPMRGRSPSPARRSRPNSP---------TKGFDGSQPFATTAEGRGSPIPRSGSPAARGRSPFRSGSPSRGDIANACDIDPEAVRLALRDFVQQLVNTERERDDALAAARSLEQRVAEMEEAAQRNEQRTQQLHRNLCETEEDKKGIDGRLASAQTALMLQEETIRRNEREKKALGDKMSGLERQISGLETEKRQLQEKLNKMKQHDARQEEDKKNLKRSLDDVENRCTQLELTKRALEGDLQRMRLALGDKDAENQVLHDRVDNLNKQIADLESKAQSLQLTIERLSVSLAKTEEDGSQQKDRVQQLNGSVSDMAATIDSLQERVGQLQKALTSSEHDRRVLQERLDNTHAAMNEAKHQNHQLLERVQSLQGELTDNEHRRNDLEDSNRQTNSLLAQKQEAEQELTQKLNKANQERAAMQERVQSLQRALAGLENEKKEIEKSASRLEKDKTALKKTLDKVERERIRTEEAASKSAFARGELDSALRRLEEENEALQRQVQALQSHLAEAEQAHSQRLMDLGAKHREETDTELERLKAGQLAAERTLENRERLHRQRVKGLEEQVATLKDQLAQEVRRR---NQFLSKSARGAAGDLSDIRTILDSSLGTVSRDPGLDAALFDAESRRLDEALDLHNAFPGGAGKRAGSPSRLGGAGGALRSSTPSGRPAGMRLSRG 2023
BLAST of Rootletin vs. TrEMBL
Match: A0A267H6I3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018782g1 PE=4 SV=1) HSP 1 Score: 1745.33 bits (4519), Expect = 0.000e+0 Identity = 1048/2017 (51.96%), Postives = 1457/2017 (72.24%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTE--LES---KMQFGDYETRKTKYGSPTNNGYGSRSENPYDNL-DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDT------------AGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD-YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENV---VTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNT--YATTTDGKGSPIPRTGSPSSKRRQLSRS--PSNCDF-NPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFM-------CGSRDQSPTRQKRIEMSKKSAAPNN--SNRRLTAG 6107
+GL+Q+N++LRKRL++E Y+RKL +Q+ QQ+QAQLVQ+LQ KVL YK+K +E LE+ K Q ++ Y ++ RS D+ DLES LI+LEEEQ+++ L N L+EQL QATQ NQ+LT D+ +LSE++QR+R +LE+RE WR+EE SF++Y +SEH+ L +L RN ++FRRQF ELK + ER++NN KNE RQ+R + AC NL+ N+KSAE++ ++ ++E + NLE+ LRE+ REL D Q ++E +L RV EL+V ++KLK Q EKDR L+ LQR+++ A +D ATR LIE+TE+MHQ+LR+I+Q V++D+D Y D D G + R + +++P FAD+TY+AVQ+A+NK++LQ+QEL+ KL S+K++ N +RQ+D++ENERRRLE+QVL +R+E+++++R + DS R+ +R K+AL ERSQLEK+RS+LN+QIE +++RL+ AN ELQR R+ LED++ D KKDL+RQ+KE+ERCQR ID LE+K+S K D+V KE +N+ LEKEVL QEK E+ DALSR E++KAELE+E+NK ++ E L + + K+ S E L + E ++ + E+E++ L EK+ LEQEK G+++EL+R EQE + L NEKA Q +E E++ E+LE+EL +NRE+L+L EQ+ + RQK ALAEELL RRE ER++N+V ++KEKE LTKEKGELVVQ+TACERE R+ E+ + L +D++QLE L++AQ+LI+QLETRK QLEGENQELI+KKENL E+ R+ +EF++E +K+ RE L + QQM+++ K A+ QE+ SH +D+ERLTK ++ R EN+R+E IH+ NMER++M+ ++E+E++++ EI QRERD+SL++AEN+KQQ+LS++EQEK I E+L T ELQ+V SELDR ++E++ +Q+QDK+ I L+ ELK FR QFE + HE EV+ LN I +LT ++ A +EV +L L++AE+ RDNLRRE+++ NR+++E EEA + KREI ELKR L EE R +E V SNE LRKKIK++E ERI+ R + + KQ++ V +EQ +EV +LR NL++VEKAR++ KRELQELRR +K LDTERGKL KEV DLQ+RVA DEEK++E RRENFGLKQKVVETEASREA+RK+L N++R DLE L+SK RDF+ +E +R++ R+L DAR+ LQ + A+NNELR AL GAEGR++ALE+ +AR + KKE+E+KLASIHSS+RRLIGFRQ+N +R R+ S + + TKGFD + +ATT +G+GSPIPR+GSP+++ R RS PS D N DI+PEAVRLALR+FVQQLV+ ER+RDDA A ARSLE R+ E EE + E R QQL + LC+ EEDKKGIDGRL+SAQTALM+QEETIRRNER++KA+ +K+S +R + +E+EKRQL+++LNK + HDAR EE+ K K+++++ ENR TQL+L KR+LEGD+QRM++AL DK+ E +VL DRV++L KQI DLE K+QSLQLTI+R+SV+LAKTEE+GS KD++QQLN S+S + ++ LQ ++ QLQKAL SSE DRR+L+ERL+++ AL+EAKHQN+ L+ER+Q +Q +L D+E+RR++LEG NRQ+N+L+ Q+QEAEQEL QK K N E+ +QERV S+QR LA E EK+EI K+ +LEKD++ALKK+L KVER++++TEE ASK+ F R ELD LRRLE+EN LQRQV LQ+ LAEAEQAH LM++ ++HR+E E+E L+A + ERTLE RER HRQRVKGLEEQ++TLKDQL E++RR + S D+R +LDSSL V++DP LDA L + E+RRLDE++ + G R SP+R + +S+ P+ + RL+ G
Sbjct: 24 RGLMQMNMDLRKRLDDEQQSYKRKLATYQEGQQRQAQLVQKLQAKVLAYKKKVSESDLENQRLKTQLRSFKQSNDSYQFKLDSETRLRSGGDEDHSSDLESALIRLEEEQQRSASLAHVNAMLREQLDQATQVNQSLTEDMKRLSEDFQRTRSDLERRESEWRDEEASFSEYFSSEHNRLLSLWRNLVSFRRQFNELKTANERDLNNFKNEMTRQSRGVHAACQNLNANLKSAETQGAVALDQERSGRANLESALRERTRELQDLQAKFEAHQADLSGRVNELSVQNEKLKFQLAEKDRTLSHLQRLRSSGGGGGGGSASRSQSAEPPGADDAATRALIEETEAMHQSLRDIAQAVISDADGYRDFSDAG-----RGRSVSPVGRGGRSPAGRQRSRSPAFADSTYSAVQAALNKRALQVQELRGKLASAKDAGDNIRRQLDESENERRRLEHQVLGLREELDTVQRAQTDSGRDYERLKSALTAREQERSQLEKVRSALNEQIESLHADLDRLQQANSELQRCRDCLEDQKADQKKDLDRQVKENERCQRCIDQLEEKLSNLKSDLVQTKENLNRACLEKEVLDQEKAEVSDALSRSELAKAELELELNKMKSEEAALRDALLKMQSLNEGLGHDKVELNKILMMYENEKAQLSGEKQALEQEKYGIREELLRCEQEKMELDNEKAGLYQTLELSEMSREKLEDELQSINRERLELAEQLNAIGRQKRALAEELLASRRETERASNTVLRVAKEKEDLTKEKGELVVQLTACEREIRRLGELASGLRADKDQLEDALHEAQELIVQLETRKGQLEGENQELIIKKENLTAEISRITKEFEVECDKLTRQRENLQQKMQQMERDLKHALAQEQQSHEDDLERLTKEKDNQRAEYENQRQETIHQNNMEREEMVSSFEREREQLHTEIAACQRERDESLMLAENDKQQALSIVEQEKSTILERLGNTHAELQTVASELDRARREAAAKQQQDKETINSLNGELKKFRDQFEQTVEEHEAEVRKLNEQINELTQQREAACREVAELKVQLRMAEDARDNLRRELIDANRRIREQEEALDAQKREIFELKRQLAEEGRAREAVEKSNEELRKKIKEMEAERIERDRSIAEQKQRIGVLEEQKAALGKEVADLRGNLREVEKARVEAKRELQELRRQVKMLDTERGKLCKEVCDLQSRVAHDEEKDEENRRENFGLKQKVVETEASREALRKELGNSKRREADLEEELRSKQRDFALAMEEARKELRRLDDARSELQLSVEAASAENNELRQALGGAEGRINALETTLARTDSVKKELEYKLASIHSSMRRLIGFRQDNPRSPSPMRGRSPSPARRSRPNSP---------TKGFDGSQPFATTAEGRGSPIPRSGSPAARGRSPFRSGSPSRGDIANACDIDPEAVRLALRDFVQQLVNTERERDDALAAARSLEQRVAEMEEAAQRNEQRTQQLHRNLCETEEDKKGIDGRLASAQTALMLQEETIRRNEREKKALGDKMSGLERQISGLETEKRQLQEKLNKMKQHDARQEEDKKNLKRSLDDVENRCTQLELTKRALEGDMQRMRLALGDKDAENQVLHDRVDNLNKQIADLESKAQSLQLTIERLSVSLAKTEEDGSQQKDRVQQLNGSVSDMAATIDSLQERVGQLQKALTSSEHDRRVLQERLDNTHAALNEAKHQNHQLLERVQSLQGELTDNEHRRNDLEGSNRQTNSLLAQKQEAEQELTQKLNKANQERAAMQERVQSLQRALAGLENEKKEIEKSASRLEKDKTALKKTLDKVERERIRTEEAASKSAFARGELDSALRRLEEENEALQRQVQALQSHLAEAEQAHSQRLMDLGAKHREETDTELERLKAGQLAAERTLENRERLHRQRVKGLEEQVATLKDQLAQEVRRR---NQFLSKSARGAAGDLSDIRTILDSSLGTVSRDPGLDAALFDAESRRLDEALDLHNAFPGGAGKRAGSPSRLGGAGGALRSSTPSGRPAGMRLSRG 2023
BLAST of Rootletin vs. TrEMBL
Match: A0A1I8FWZ5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 1714.51 bits (4439), Expect = 0.000e+0 Identity = 1039/2017 (51.51%), Postives = 1444/2017 (71.59%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTE--LES---KMQFGDYETRKTKYGSPTNNGYGSRSENPYDNL-DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDT------------AGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSD-YFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENV---VTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNT--YATTTDGKGSPIPRTGSPSSKRRQLSRS--PSNCDF-NPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFM-------CGSRDQSPTRQKRIEMSKKSAAPNN--SNRRLTAG 6107
+GL+Q+N++LRKRL++E Y+RKL +Q+ QQ+QAQLVQ+LQ KVL YK+K +E LE+ K Q ++ Y ++ RS D+ DLES LI+LEEEQ+++ L N L+EQL QATQ NQ+LT D+ +LSE++QR+R +LE+RE WR+EE SF++Y +SEH+ L +L RN ++FRRQF ELK + ER++NN KNE RQ+R + AC NL+ N+KSAE++ ++ ++E + NLE+ LRE+ REL D Q ++E +L RV EL+V ++KLK Q EKDR L+ LQR+++ A +D ATR LIE+TE+MHQ+LR+I+Q V++D+D Y D D G + R + +++P FAD+TY+AVQ+A+NK++LQ+QEL+ KL S+K++ N +RQ+D++ENERRRLE+QVL +R+E+++++R + DS R+ +R K+AL ERSQLEK+RS+LN+QIE +++RL+ AN ELQR R+ LED++ D KKDL+RQ+KE+ERCQR ID LE+K+S K D+V KE +N+ LEKEVL QEK E+ DALSR E++KAELE+E+NK ++ E L + + K+ S E L + E ++ + E+E++ L EK+ LEQEK G+++EL+R EQE + L NEKA Q +E E++ E+LE+EL +NRE+L+L EQ+ + RQK ALAEELL RRE ER++N+V ++KEKE LTKEKGELVVQ+TACERE R+ E+ + L +D++QLE L++AQ+LI+QLETRK QLEGENQELI+KKENLN E+ R+ +EF++E +K+ RE L + QQM+++ K A+ QE+ SH +D+ERLTK ++ R EN+R+E+IH+ NMER++M+ ++E+E++++ EI QRERD+SL++AEN+KQQ+LS++EQEK I E+L T ELQ+V SELDR ++E++ +Q+QDK+ I L+ ELK FR QFE + HE EV+ LN I +LT ++ A +EV +L L++AE+ RDNLRRE+++ NR+++E EEA + KREI ELKR L EE R +E V SNE LRKK ++ V +EQ +EV +LR NL++VEKAR++ KRELQELRR +K LDTERGKL KEV DLQ+RVA DEEK++E RRENFGLKQKVVETEASREA+RK+L N++R DLE L+SK RDF+ +E +R++ R+L DAR+ LQ + A+NNELR AL GAEGR++ALE+ +AR + KKE+E+KLASIHSS+RRLIGFRQ+N +R R+ S + + TKGFD + +ATT +G+GSPIPR+GSP+++ R RS PS D N DI+PEAVRLALR+FVQQLV+ ER+RDDA A ARSLE R+ E EE + E R QQL + LC+ EEDKKGIDGRL+SAQTALM+QEETIRRNER++KA+ +K+S +R + +E+EKRQL+++LNK + HDAR EE+ K K+++++ ENR TQL+L KR+LEGDLQRM++AL DK+ E +VL DRV++L KQI DLE K+QSLQLTI+R+SV+LAKTEE+GS KD++QQLN S+S + ++ LQ ++ QLQKAL SSE DRR+L+ERL+++ A++EAKHQN+ L+ER+Q +Q +L D+E+RR++LE NRQ+N+L+ Q+QEAEQEL QK K N E+ +QERV S+QR LA E EK+EI K+ +LEKD++ALKK+L KVER++++TEE ASK+ F R ELD LRRLE+EN LQRQV LQ+ LAEAEQAH LM++ ++HR+E E+E L+A + ERTLE RER HRQRVKGLEEQ++TLKDQL E++RR + S D+R +LDSSL V++DP LDA L + E+RRLDE++ + G R SP+R + +S+ P+ + RL+ G
Sbjct: 131 RGLMQMNMDLRKRLDDEQQSYKRKLATYQEGQQRQAQLVQKLQAKVLAYKKKVSESDLENQRLKTQLRSFKQSNDSYQFKLDSETRLRSGGDEDHSSDLESALIRLEEEQQRSASLAHVNAMLREQLDQATQVNQSLTEDMKRLSEDFQRTRSDLERRESEWRDEEASFSEYFSSEHNRLLSLWRNLVSFRRQFNELKTANERDLNNFKNEMTRQSRGVHAACQNLNANLKSAETQGAVALDQERSGRANLESALRERTRELQDLQAKFEAHQADLSGRVNELSVQNEKLKFQLAEKDRTLSHLQRLRSSGGGGGGGSASRSQSAEPPGADDAATRALIEETEAMHQSLRDIAQAVISDADGYRDFSDAG-----RGRSVSPVGRGGRSPAGRQRSRSPAFADSTYSAVQAALNKRALQVQELRGKLASAKDAGDNIRRQLDESENERRRLEHQVLGLREELDTVQRAQTDSGRDYERLKSALTAREQERSQLEKVRSALNEQIESLHADLDRLQQANSELQRCRDCLEDQKADQKKDLDRQVKENERCQRCIDQLEEKLSNLKSDLVQTKENLNRACLEKEVLDQEKAEVSDALSRSELAKAELELELNKMKSEEAALRDALLKMQSLNEGLGHDKVELNKILMMYENEKAQLSGEKQALEQEKYGIREELLRCEQEKMELDNEKAGLYQTLELSEMSREKLEDELQSINRERLELAEQLNAIGRQKRALAEELLASRRETERASNTVLRVAKEKEDLTKEKGELVVQLTACEREIRRLGELASGLRADKDQLEDALHEAQELIVQLETRKGQLEGENQELIIKKENLNAEISRITKEFEVECDKLTRQRENLQQKMQQMERDLKHALAQEQQSHEDDLERLTKEKDNQRAEYENQRQEMIHQNNMEREEMVSSFEREREQLHTEIAACQRERDESLMLAENDKQQALSIVEQEKSTILERLGNTHAELQTVASELDRARREAAAKQQQDKETINSLNGELKKFRDQFEQTVEEHEAEVRKLNEQINELTQQREAACREVAELKVQLRMAEDARDNLRRELIDANRRIREQEEALDAQKREIFELKRQLAEEGRAREAVEKSNEELRKK--------------------RIGVLEEQKAALGKEVADLRGNLREVEKARVEAKRELQELRRQVKMLDTERGKLCKEVCDLQSRVAHDEEKDEENRRENFGLKQKVVETEASREALRKELGNSKRREADLEEELRSKQRDFALAMEDARKELRRLDDARSELQLSVEAASAENNELRQALGGAEGRINALETTLARTDSVKKELEYKLASIHSSMRRLIGFRQDNPRSPSPMRGRSPSPARRSRPNSP---------TKGFDGSQPFATTAEGRGSPIPRSGSPAARGRSPFRSGSPSRGDIANACDIDPEAVRLALRDFVQQLVNTERERDDALAAARSLEQRVAEMEEAAQRNEQRTQQLHRNLCETEEDKKGIDGRLASAQTALMLQEETIRRNEREKKALGDKMSGLERQISGLETEKRQLQEKLNKMKQHDARQEEDKKNLKRSLDDVENRCTQLELTKRALEGDLQRMRLALGDKDAENQVLHDRVDNLNKQIADLESKAQSLQLTIERLSVSLAKTEEDGSQQKDRVQQLNGSVSDMAATIDSLQERVGQLQKALTSSEHDRRVLQERLDNTHAAMNEAKHQNHQLLERVQSLQGELTDNEHRRNDLEDSNRQTNSLLAQKQEAEQELTQKLNKANQERAAMQERVQSLQRALAGLENEKKEIEKSASRLEKDKTALKKTLDKVERERIRTEEAASKSAFARGELDSALRRLEEENEALQRQVQALQSHLAEAEQAHSQRLMDLGAKHREETDTELERLKAGQLAAERTLENRERLHRQRVKGLEEQVATLKDQLAQEVRRR---NQFLSKSARGAAGDLSDIRTILDSSLGTVSRDPGLDAALFDAESRRLDEALDLHNAFPGGAGKRAGSPSRLGGAGGALRSSTPSGRPAGMRLSRG 2110
BLAST of Rootletin vs. TrEMBL
Match: A0A1S3JC29 (rootletin-like isoform X4 OS=Lingula unguis OX=7574 GN=LOC106171578 PE=4 SV=1) HSP 1 Score: 1665.59 bits (4312), Expect = 0.000e+0 Identity = 1025/2010 (51.00%), Postives = 1450/2010 (72.14%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESK-MQFGDYETRKTKYGSPTNNGYGSRSENPYDNL---------DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEI-------NMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFET--VXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQE----NVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSP----SSKRRQLSRSPSNCD--FNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKRIEMSKKSAAPNNSNRRLTAG 6107
K L+ N+ELR++LEEEH Y+RKL +QD QQ+QAQLVQ+LQ KVLQYK+KC ELE++ QF + + T + SR E L DLE+ LIKLEEEQ++++ L N L+EQL QAT +NQ LT+DI +L+ +WQR+REELEQ+E WR+EE SFN+Y ++EHS L +L R + FRRQF E+K++TER++++++ + R +R + +AC NLS N++S +++ Q+S +R+ D+ LENQLR+K RE+ + QT+++ +EL +V ELT++++KLKIQ +EKD++++ LQ + AG + +ME +A++ + E+TE++H ALR+I+Q V++D+D ED G T + ++ R R+ + ++ +++P FAD+T++AVQ+A+NK+ LQ+QE+++K N+SK+ ++Q+DD ENERRR+E Q++ R+E+++ RRDK+DSFR+ DRFKN L++T +E+SQLE++R +LN+QIE TE E+L++AN E+QR R+ +EDE++D KD ERQIKE+ERC + I+ LE K+S+ +ED V KE ++K QL+K+VL EK E+G+ALS+ E+ AELE+ VNK +T E L + + K+ + E L + E ++ I QLE E++SL EK + EKSG+++ELVR EQE + L EK Q +E E E L+EE++ LNREK+++TEQ+ + RQKSALAEELLQ R+E ER +++V ++KEKE LTKEK L+VQ+T+ ERENRQQSE+IA +D+E LE LY+AQQL QLETRKEQLEGENQELI++KE L E+ RLR+E +LE EK+ +E + + Q +++ +++ QE+ +H ED+ERLT+ E+ R E REE++H ME+++MI E EK+++ QE+ IQRERDDS+L AENEKQQ LSL+EQEK + EKL TQ +LQS E +R+K+ES +RQE DK +I GL +ELK FRSQFE+ + E+K LN +++L K+QAL+EV +L LK+ EETRD +RR+++E NRK++EA+E KE +++EI +LKR + +E REKE V +NE LR KIK E E+I+L R + ++KQ++ + +EQ ++E ++LR +L++VEKARLD +RELQELRR +K LD+ER KL KEV DLQ RVARDEEKE+E+RRE +GLKQKVVETEASREA+RK+LAN QR +LE+ + K++D+ +E SRR+ RKL + R +L+ + + EL++ALSGAEGRV+ALE+ +ARVEGAK+++EFKL+SIHS+LRR IGFRQ+ T+R+R+ S R S KGFDNT+ TTTDG+GSPI RTGSP S RR+ SPS D + DI+PEAVR+ALR+FVQQL +ER+RDD +Q RSL +LKE EE + E RLQQLQ++L +AEEDK+GID RL+SAQTALM+QEETIRRNER+RK MAEK++ +R A+MESEKRQL++R+ K + ++A+LEE+ KQ + A++++E R T+ +LAKRS+EGDLQR+K+A+ DKETE +VL +R+++L +Q+QDL+ K QSLQLTIDR+S AL+KTEEE S KDK+Q LNMSLS S + LQ ++ QLQ+AL SSE DRR+L+ERL++++ A +EAK QN+ LMER+Q IQ++L+DSE RR+ELEG RQ++ +IVQRQE EQELNQ+ QKL +EK LQERV S+QR A E +K+EI +N + EKD+SALKK+L KVER+K+KTEE+AS+++ E+ ++ L+RLE+EN +LQRQ+ +Q Q+AE EQAH L+++ +RHR E ME E LR++ +Q ERTLE RERAHRQRVKGLEEQI+TLKDQL EM++R STR D D+R++LDSSL VA+DP LD +LL+ E +L +++ + SPTR +S + +P S + G
Sbjct: 39 KSLVTQNVELRRKLEEEHASYKRKLQAYQDGQQRQAQLVQKLQAKVLQYKKKCGELEAQNSQFKSESQQVKRSLQSTQDVLQSRLETTEARLRMAEEDASMDLETALIKLEEEQQRSSSLAHVNSMLREQLDQATSANQQLTQDIHRLTTDWQRAREELEQKESEWRDEEQSFNEYFSAEHSRLLSLWRAVVAFRRQFGEMKSATERDLSHIRADLTRTSRSMHSACLNLSANLRSMDTQSQVSADRDRSDRNALENQLRDKVREVMELQTKFDIHTSELNSKVNELTMMNEKLKIQLIEKDKSISTLQANMDRVDAGRMEAARERESMEVEASQTMREETEALHAALRDIAQAVISDADQSKLEDEGASTAELARSMSPSRSPRMRTRTRSASPSRSPAFADSTFSAVQAALNKRQLQVQEMRAKFNASKDQNAALRKQLDDTENERRRIERQMMEYREELDNARRDKEDSFRDRDRFKNQLDLTGSEKSQLERMRQALNEQIEALNTENEKLQAANSEIQRQRDNIEDEKDDVIKDKERQIKENERCHKVIEQLEMKLSKLREDFVETKETLHKSQLDKDVLENEKAEMGEALSKAELRMAELELAVNKLKTEEAGLRDALLKMQALNEGLGQDKIELNKIIMQLEAEKASLFGEKHAIIAEKSGIQEELVRVEQEKMDLDTEKLGLNQTVEIHEQTVENLQEEITILNREKVEITEQLNSVSRQKSALAEELLQSRKECERQSDTVVRIAKEKEDLTKEKASLIVQLTSSERENRQQSEIIAGFKADKESLENALYEAQQLNAQLETRKEQLEGENQELILRKEALQAEIARLRKEMELEIEKLERQKESITAKLGQTERDLNVSLTQEKQAHEEDIERLTREKETQRIEYEANREEILHHNAMEKEEMIARAENEKEQLQQELAAIQRERDDSMLFAENEKQQVLSLMEQEKSTLAEKLNSTQKDLQSQIVENERLKRESMSRQESDKNSINGLQAELKKFRSQFEEATVAQDRELKDLNGRMQELQRQKEQALREVAELKVQLKMVEETRDTIRRDLIEANRKIREADETKELMRKEIGDLKRSVNDEVREKEAVQKANEELRNKIKRAEGEKIELSRNLQEAKQRIGILEEQKTGVQKEASDLRASLREVEKARLDARRELQELRRQVKMLDSERSKLGKEVGDLQNRVARDEEKEEESRREQYGLKQKVVETEASREALRKELANLQRRYAELEDESRVKEKDYQMALEDSRRNERKLEEIRRNLETNLEACNNEIGELKLALSGAEGRVNALEAQLARVEGAKRDVEFKLSSIHSTLRRTIGFRQDMPRSPSPTMRARSPSPRRTRPTSP----------AKGFDNTFTTTTDGRGSPIARTGSPDRARSPTRRERDMSPSRLDQSVSIADIDPEAVRIALRDFVQQLAGSERERDDCLSQVRSLSMQLKEMEEDRDRTEARLQQLQKSLGEAEEDKRGIDSRLASAQTALMLQEETIRRNERERKTMAEKINTLERGLAAMESEKRQLQERVGKMKQNEAKLEEDKKQLRMALDDSEQRCTKTELAKRSMEGDLQRLKLAMNDKETENQVLQERIDNLTRQMQDLDSKCQSLQLTIDRLSNALSKTEEEESVQKDKVQVLNMSLSEQSATIADLQERVQQLQRALTSSEHDRRVLQERLDTTRQAQNEAKKQNHSLMERVQAIQNELSDSEVRRNELEGQVRQAHGIIVQRQEQEQELNQRLQKLGTEKQGLQERVASLQRQYAQLETDKREIERNVIRTEKDKSALKKTLDKVEREKMKTEEVASRSMTEKGSMEGTLKRLEEENMDLQRQLQSMQAQMAEMEQAHSQRLIDLTTRHRSETEMETERLRSAQLQAERTLEARERAHRQRVKGLEEQINTLKDQLAQEMRKRQ-QFIQRSTRTG---DDIKDIRNILDSSLGTVARDPGLDTLLLDHETSKLGDTVDHHST--ISPTR-----LSPRRRSPTRSTSPVRLG 2027
BLAST of Rootletin vs. TrEMBL
Match: A0A1S3HNJ8 (rootletin-like isoform X5 OS=Lingula unguis OX=7574 GN=LOC106156388 PE=4 SV=1) HSP 1 Score: 1665.59 bits (4312), Expect = 0.000e+0 Identity = 1023/2010 (50.90%), Postives = 1452/2010 (72.24%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYG-SPTNNGYGSRSENPYDNL---------DLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEI-------NMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFET--VXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQE----NVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSP----SSKRRQLSRSPSNCD--FNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKRIEMSKKSAAPNNSNRRLTAG 6107
K L+ N+ELR++LEEEH Y+RKL +QD QQ+QAQLVQ+LQ KVLQYK+KC ++E +Q + E ++++ T + SR E L DLE+ LIKLEEEQ++++ L N L+EQL QAT +NQ LT+DI +L+ +WQR+REELEQ+E WR+EE SFN+Y ++EHS L +L R + FRRQF E+K++TER++++++ + R +R + +AC NLS N++S +++ Q+S +R+ D+ LENQLR+K RE+ + QT+++ +EL +V ELT++++KLKIQ +EKD++++ LQ + AG + +ME +A++ + E+TE++H ALR+I+Q V++D+D ED G T + ++ R R+ + ++ +++P FAD+T++AVQ+A+NK+ LQ+QE+++K N+SK+ ++Q+DD ENERRR+E Q++ R+E+++ RRDK+DSFR+ DRFKN L++T +E+SQLE++R +LN+QIE TE E+L++AN E+QR R+ +EDE++D KD ERQIKE+ERC + I+ LE K+S+ +ED V KE ++K QL+K+VL EK E+G+ALS+ E+ AELE+ VNK +T E L + + K+ + E L + E ++ I QLE E++SL EK + EKSG+++ELVR EQE + L EK Q +E E E L+EE++ LNREK+++TEQ+ + RQKSALAEELLQ R+E ER +++V ++KEKE LTKEK L+VQ+T+ ERENRQQSE+IA +D+E LE LY+AQQL QLETRKEQLEGENQELI++KE L E+ RLR+E +LE EK+ +E + + Q +++ +++ QE+ +H ED+ERLT+ E+ R E REE++H ME+++MI E EK+++ QE+ IQRERDDS+L AENEKQQ LSL+EQEK + EKL TQ +LQS E +R+K+ES +RQE DK +I GL +ELK FRSQFE+ + E+K LN +++L K+QAL+EV +L LK+ EETRD +RR+++E NRK++EA+E KE +++EI +LKR + +E REKE V +NE LR KIK E E+I+L R + ++KQ++ + +EQ ++E ++LR +L++VEKARLD +RELQELRR +K LD+ER KL KEV DLQ RVARDEEKE+E+RRE +GLKQKVVETEASREA+RK+LAN QR +LE+ + K++D+ +E SRR+ RKL + R +L+ + + EL++ALSGAEGRV+ALE+ +ARVEGAK+++EFKL+SIHS+LRR IGFRQ+ T+R+R+ S R S KGFDNT+ TTTDG+GSPI RTGSP S RR+ SPS D + DI+PEAVR+ALR+FVQQL +ER+RDD +Q RSL +LKE EE + E RLQQLQ++L +AEEDK+GID RL+SAQTALM+QEETIRRNER+RK MAEK++ +R A+MESEKRQL++R+ K + ++A+LEE+ KQ + A++++E R T+ +LAKRS+EGDLQR+K+A+ DKETE +VL +R+++L +Q+QDL+ K QSLQLTIDR+S AL+KTEEE S KDK+Q LNMSLS S + LQ ++ QLQ+AL SSE DRR+L+ERL++++ A +EAK QN+ LMER+Q IQ++L+DSE RR+ELEG RQ++ +IVQRQE EQELNQ+ QKL +EK LQERV S+QR A E +K+EI +N + EKD+SALKK+L KVER+K+KTEE+AS+++ E+ ++ L+RLE+EN +LQRQ+ +Q Q+AE EQAH L+++ +RHR E ME E LR++ +Q ERTLE RERAHRQRVKGLEEQI+TLKDQL EM++R STR D D+R++LDSSL VA+DP LD +LL+ E +L +++ + SPTR +S + +P S + G
Sbjct: 39 KSLVTQNVELRRKLEEEHASYKRKLQAYQDGQQRQAQLVQKLQAKVLQYKKKCGQMEDILQQKELELHRSRFSLQSTQDVLQSRLETTEARLRMAEEDASMDLETALIKLEEEQQRSSSLAHVNSMLREQLDQATSANQQLTQDIHRLTTDWQRAREELEQKESEWRDEEQSFNEYFSAEHSRLLSLWRAVVAFRRQFGEMKSATERDLSHIRADLTRTSRSMHSACLNLSANLRSMDTQSQVSADRDRSDRNALENQLRDKVREVMELQTKFDIHTSELNSKVNELTMMNEKLKIQLIEKDKSISTLQANMDRVDAGRMEAARERESMEVEASQTMREETEALHAALRDIAQAVISDADQSKLEDEGASTAELARSMSPSRSPRMRTRTRSASPSRSPAFADSTFSAVQAALNKRQLQVQEMRAKFNASKDQNAALRKQLDDTENERRRIERQMMEYREELDNARRDKEDSFRDRDRFKNQLDLTGSEKSQLERMRQALNEQIEALNTENEKLQAANSEIQRQRDNIEDEKDDVIKDKERQIKENERCHKVIEQLEMKLSKLREDFVETKETLHKSQLDKDVLENEKAEMGEALSKAELRMAELELAVNKLKTEEAGLRDALLKMQALNEGLGQDKIELNKIIMQLEAEKASLFGEKHAIIAEKSGIQEELVRVEQEKMDLDTEKLGLNQTVEIHEQTVENLQEEITILNREKVEITEQLNSVSRQKSALAEELLQSRKECERQSDTVVRIAKEKEDLTKEKASLIVQLTSSERENRQQSEIIAGFKADKESLENALYEAQQLNAQLETRKEQLEGENQELILRKEALQAEIARLRKEMELEIEKLERQKESITAKLGQTERDLNVSLTQEKQAHEEDIERLTREKETQRIEYEANREEILHHNAMEKEEMIARAENEKEQLQQELAAIQRERDDSMLFAENEKQQVLSLMEQEKSTLAEKLNSTQKDLQSQIVENERLKRESMSRQESDKNSINGLQAELKKFRSQFEEATVAQDRELKDLNGRMQELQRQKEQALREVAELKVQLKMVEETRDTIRRDLIEANRKIREADETKELMRKEIGDLKRSVNDEVREKEAVQKANEELRNKIKRAEGEKIELSRNLQEAKQRIGILEEQKTGVQKEASDLRASLREVEKARLDARRELQELRRQVKMLDSERSKLGKEVGDLQNRVARDEEKEEESRREQYGLKQKVVETEASREALRKELANLQRRYAELEDESRVKEKDYQMALEDSRRNERKLEEIRRNLETNLEACNNEIGELKLALSGAEGRVNALEAQLARVEGAKRDVEFKLSSIHSTLRRTIGFRQDMPRSPSPTMRARSPSPRRTRPTSP----------AKGFDNTFTTTTDGRGSPIARTGSPDRARSPTRRERDMSPSRLDQSVSIADIDPEAVRIALRDFVQQLAGSERERDDCLSQVRSLSMQLKEMEEDRDRTEARLQQLQKSLGEAEEDKRGIDSRLASAQTALMLQEETIRRNERERKTMAEKINTLERGLAAMESEKRQLQERVGKMKQNEAKLEEDKKQLRMALDDSEQRCTKTELAKRSMEGDLQRLKLAMNDKETENQVLQERIDNLTRQMQDLDSKCQSLQLTIDRLSNALSKTEEEESVQKDKVQVLNMSLSEQSATIADLQERVQQLQRALTSSEHDRRVLQERLDTTRQAQNEAKKQNHSLMERVQAIQNELSDSEVRRNELEGQVRQAHGIIVQRQEQEQELNQRLQKLGTEKQGLQERVASLQRQYAQLETDKREIERNVIRTEKDKSALKKTLDKVEREKMKTEEVASRSMTEKGSMEGTLKRLEEENMDLQRQLQSMQAQMAEMEQAHSQRLIDLTTRHRSETEMETERLRSAQLQAERTLEARERAHRQRVKGLEEQINTLKDQLAQEMRKRQ-QFIQRSTRTG---DDIKDIRNILDSSLGTVARDPGLDTLLLDHETSKLGDTVDHHST--ISPTR-----LSPRRRSPTRSTSPVRLG 2027
BLAST of Rootletin vs. Ensembl Cavefish
Match: crocc2 (rootletin-like [Source:NCBI gene;Acc:103030367]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 617/1895 (32.56%), Postives = 1052/1895 (55.51%), Query Frame = 3
Query: 198 KRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSR----SENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQRE-KIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNL--SCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATR-VLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEK-AIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQ-----SKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
+RL +E YR KL +QDSQ +QAQLV +LQ KVLQYK++C E+E ++ E K + T+ + SE + +LD +S L LEEE+++ + LT+ N L+EQL QA ++NQ LT E ++EE EQR+ ++ R +EMS + L E + ++ L R + R F +L+A +R +++++ EF R L AC NL N SA + ++S LE QL++K +E Q R++ E EL R+ EL + +L+ QN EKD L ALQ + D EDKA + L ++ + L +I + V +D D ++ G E + P D T +VQ A++++ Q+L++ L+++ + + ++ D+E+E R+LE ++ ++ E + + R+A R++ +L + ++E+ +LEK Q+ E+ERL+S++ ++QR R+ L ++ED ++ L R+ E+ER R+I+ LE K S ++++V +EA+++ L+KEVL EK + ALS+ E AE E+ + K Q+ E L ++ K+++ E L + E +R + Q+E E++ L KR E E++ ++EL +QE + + EK A++ + E E + L EQ+ + QK AL E+L C+RE E +S+ +E+E L ++K L VQ+TA +R + ++ +A+ + E LE+ ++D Q+ I LE +LEGE L E L E+ R++ E + + + + R+ L + Q ++ + ++ + SH E +E K ES R +RE++ + +E +++ + Q++ +E+ Q+ DSLL AE+ KQQ+LS E EK + E+++ Q ++++ E +R++++ N+QEQDK AGL SEL+ R FE+ + E+ K LN I++L+L + +A +EV L + EE+RD +R+E++E +L+E + +E +RE EL+R L E REKE + SN+ LR +K E+E L R + + +Q++ V +E + +QE ++RN+++++EK+ L +RELQELRR +K L+ + G+ +EV +LQ R+ ++E++E+EAR+E+F LKQ+V+E++A REA K++A QR + +LE V + + RD +S+ +S RR + A +LQ+V + N EL++ AEGR +LE +A + ++ ++E +L+ + S+LRR +G + R+R+ + P R P KG ++ G P+ PS SP + + +D+ E+V+ ALR F Q+L DA+R+RDDA AQ SL+ +L E + E + R QQL+ ++ D E+ K+ + RL + Q +L +QEE RR +R+R+ M E+LS + ++ R L++RL + +A L E ++ K+ + AE R ++L+L+ R+LEG+LQR ++ + E E L +R++SL +++ + E + L++ +R + ALA+ E + + ++K+Q +L + + +QLQ L +SE + R L+ERLE + AL ++K QN L ER+Q +Q + E R SEL+ + QR++ +E L ++ LQERV ++Q +L + ++ ++ K +L KD++ALK++L KVE+++ + EE ++ EL+Q L LE + + + L++QL+ EQAH L+E+ +RHRQE+ +E E LR S Q ER LE RE+AHRQRV+ LEEQ+ TLK+QL EM+RR
Sbjct: 140 QRLRDESRAYRLKLQAYQDSQHRQAQLVHKLQTKVLQYKKRCGEMEQQVLEKTSELEKLRLSLQTHLDSSAHRFQLSEQEH-SLDAQSKLALLEEERRRCSGLTKVNALLREQLEQANEANQALT-------ESLHEAQEEAEQRDIRLRREQEMSAS-RLGREQARVRALWRQAASLRSTFTQLRAFADRNLSDIRGEFANANRKLHVACLNLQSGINEHSAPAGLEVSA---------LEKQLKDKLKEAMQLQGRFDAEKVELNSRIVELAEMVKQLRAQNSEKDSCLAALQ--SSLDRMEVSQAEDKAEKDTLRKEISGLQMILHSIGKAVCDDGDSSSSDSPGEELPSPVPTV-----------------SSPQRDNTLISVQRALSQRQKLTQDLQTCLDAALDQAAVLRDRLQDSESECRQLEQRIQQLQQENRHAEKAGVEISRDAQRYRTSLGLITSEKGELEKHLFVAQHQLNHDQAELERLRSSSLDVQRQRDILRQQREDLERQLARERSEAERGLRTIEQLEAKHSDVRKELVTVREALSQITLQKEVLEDEKASLEKALSKAEAQNAEQELALTKLQSEEAGLRDSFEKMAALCEGLAKDKVELNRVLLQVEAEKAELSERKREAELERAAAREELALIQQELMDVVAEKRALEASHVLLQESRAGLDTELTLLQREKSSVL-EQLAQVSEQKQALMEQLSVCQRESELQTSSLQRTGREREELARDKAGLSVQLTAEQRRAKTLAQELAAARVEWESLESAVFDGQEQISALEADLSRLEGERGNLQQANEALTREVSRIQSELEQQVSQALLERQALELKLTQAERNAQRSLASAQQSHQEQLEAERKDKESVRLALTAQREQVEEQLRVEYEELRLRSRTDLQQLQEELARAQQHCTDSLLQAESHKQQALSEKEAEKTVLTERILSLQRDIETTALETERMRRDFLNKQEQDKDTAAGLQSELQQLRLHFEESLSSREISEKRLNDQIRELSLQRHEAQQEVEQLRRSVLEVEESRDGVRKELVEARAELRECVQEREAGRRESLELRRALGNESREKEAIHTSNQELRTSVKRAESENNGLKRTLEEREQRLTVLEECKSSLQQEALKVRNSMRELEKSHLQARRELQELRRKVKTLEGQCGQQEQEVGELQARLVQEEQREEEARKESFSLKQRVLESDAGREAALKEVAGLQRRVAELEEVQRVEKECQRQRD-ASLQDSQRRQREETARLEGALQEVQAELREQNVELKL----AEGRGRSLEEQLALADASRSDLEQRLSGLSSALRRTLG------IGCRARSPTPRPRGRRPSPWRNRSPE---KGSESVS-------GGPMGSVQDPSV-------SPVHGEDGKLDV--ESVQAALRNFQQELRDAQRERDDAQAQVMSLKRQLSELQAGQEKSSARAQQLKHSIKDLEQGKRDLAERLEAVQASLSLQEEAGRRGDRERRVMEEELSRLKNGLQASDAGARALQERLERVEQSEAGLGAECRRLKECWDAAEARASRLELSHRTLEGELQRSQIRSAELEAELSSLQERMSSLRRELGESEECTALLKVEGERAATALARAELQEAQLREKLQSTAAALKESCSSAGAAKEQTAQLQSTLTASELEGRTLQERLEKMREALSDSKKQNYALSERLQNLQRTQEEYELRVSELDKHTHSLQEALRQREQVVEEARADCTVLQKDRVELQERVTNLQTSLHSLQDDRTQLDKILTRLGKDKAALKRTLQKVEQERRRQEEEVLCVGRQKEELEQSLSSLEQQLSDTHAESHTLKSQLSHLEQAHSQRLLEVTARHRQELELETERLRGSQQQAERALEAREKAHRQRVRCLEEQVCTLKEQLEQEMRRR 1966
BLAST of Rootletin vs. Ensembl Cavefish
Match: crocc2 (rootletin-like [Source:NCBI gene;Acc:103030367]) HSP 1 Score: 633.639 bits (1633), Expect = 0.000e+0 Identity = 606/1895 (31.98%), Postives = 1030/1895 (54.35%), Query Frame = 3
Query: 198 KRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSR----SENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQRE-KIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNL--SCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATR-VLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEK-AIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQ-----SKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRR 5840
+RL +E YR KL +QDSQ +QAQLV +LQ KVLQYK++C E+E ++ E K + T+ + SE + +LD +S L LEEE+++ + LT+ N L+EQL QA ++NQ LT E ++EE EQR+ ++ R +EMS + L E + ++ L R + R F +L+A +R +++++ EF R L AC NL N SA + ++S LE QL++K +E Q R++ E EL R+ EL + +L+ QN EKD L ALQ + D EDKA + L ++ + L +I + V +D D ++ G E + P D T +VQ A++++ Q+L++ L+++ + + ++ D+E+E R+LE ++ ++ E + + R+A R++ +L + ++E+ +LEK Q+ E+ERL+S++ ++QR R+ L ++ED ++ L R+ E+ER R+I+ LE K S ++++V +EA+++ L+KEVL EK + ALS+ E AE E+ + K Q+ E L ++ K+++ E L + E +R + Q+E E++ L KR E E++ ++EL +QE + + EK A++ + E E + L EQ+ + QK AL E+L C+RE E +S+ +E+E L ++K L VQ+TA +R + ++ +A+ + E LE+ ++D Q+ I LE +LEGE L E L E+ R++ E + + + + R+ L + Q ++ + ++ + SH E +E K ES R +RE++ + +E +++ + Q++ +E+ Q+ DSLL AE+ KQQ+LS E EK + E+++ Q ++++ E +R++++ N+QEQDK AGL SEL+ R FE+ + E+ K LN I++L+L + +A +EV L + EE+RD +R+E++E +L+E + +E +RE EL+R L E REKE + SN+ LR +K E+E L R + + +Q++ V +E + +QE ++RN+++++EK+ L +RELQELRR +K L+ + G+ +EV +LQ R+ ++E++E+EAR+E+F LKQ+V+E++A REA K++A QR + +LE V + + RD +S+ +S RR + A +LQ+V + N EL++ AEGR +LE +A + ++ ++E +L+ + S+LRR +G + R+R+ + P R P KG ++ G P+ PS SP + + +D+ E+V+ ALR F Q+L DA+R+RDDA AQ SL+ +L E + E + R QQL+ ++ D E+ K+ + RL + Q +L +QEE RR +R+R+ M E+LS + ++ R L++RL + +A L E ++ K+ + AE R ++L+L+ R+LEG+LQR ++ + E E L +R++SL +++ + E + L++ +R + ALA+ E + + ++K+Q +L + + +QLQ L +SE + R L+ERLE + AL ++K QN L ER+Q +Q + E R SEL+ + QR++ +E L ++ LQERV ++Q +L + ++ ++ K +L KD++ALK++L K +QL+ EQAH L+E+ +RHRQE+ +E E LR S Q ER LE RE+AHRQRV+ LEEQ+ TLK+QL EM+RR
Sbjct: 140 QRLRDESRAYRLKLQAYQDSQHRQAQLVHKLQTKVLQYKKRCGEMEQQVLEKTSELEKLRLSLQTHLDSSAHRFQLSEQEH-SLDAQSKLALLEEERRRCSGLTKVNALLREQLEQANEANQALT-------ESLHEAQEEAEQRDIRLRREQEMSAS-RLGREQARVRALWRQAASLRSTFTQLRAFADRNLSDIRGEFANANRKLHVACLNLQSGINEHSAPAGLEVSA---------LEKQLKDKLKEAMQLQGRFDAEKVELNSRIVELAEMVKQLRAQNSEKDSCLAALQ--SSLDRMEVSQAEDKAEKDTLRKEISGLQMILHSIGKAVCDDGDSSSSDSPGEELPSPVPTV-----------------SSPQRDNTLISVQRALSQRQKLTQDLQTCLDAALDQAAVLRDRLQDSESECRQLEQRIQQLQQENRHAEKAGVEISRDAQRYRTSLGLITSEKGELEKHLFVAQHQLNHDQAELERLRSSSLDVQRQRDILRQQREDLERQLARERSEAERGLRTIEQLEAKHSDVRKELVTVREALSQITLQKEVLEDEKASLEKALSKAEAQNAEQELALTKLQSEEAGLRDSFEKMAALCEGLAKDKVELNRVLLQVEAEKAELSERKREAELERAAAREELALIQQELMDVVAEKRALEASHVLLQESRAGLDTELTLLQREKSSVL-EQLAQVSEQKQALMEQLSVCQRESELQTSSLQRTGREREELARDKAGLSVQLTAEQRRAKTLAQELAAARVEWESLESAVFDGQEQISALEADLSRLEGERGNLQQANEALTREVSRIQSELEQQVSQALLERQALELKLTQAERNAQRSLASAQQSHQEQLEAERKDKESVRLALTAQREQVEEQLRVEYEELRLRSRTDLQQLQEELARAQQHCTDSLLQAESHKQQALSEKEAEKTVLTERILSLQRDIETTALETERMRRDFLNKQEQDKDTAAGLQSELQQLRLHFEESLSSREISEKRLNDQIRELSLQRHEAQQEVEQLRRSVLEVEESRDGVRKELVEARAELRECVQEREAGRRESLELRRALGNESREKEAIHTSNQELRTSVKRAESENNGLKRTLEEREQRLTVLEECKSSLQQEALKVRNSMRELEKSHLQARRELQELRRKVKTLEGQCGQQEQEVGELQARLVQEEQREEEARKESFSLKQRVLESDAGREAALKEVAGLQRRVAELEEVQRVEKECQRQRD-ASLQDSQRRQREETARLEGALQEVQAELREQNVELKL----AEGRGRSLEEQLALADASRSDLEQRLSGLSSALRRTLG------IGCRARSPTPRPRGRRPSPWRNRSPE---KGSESVS-------GGPMGSVQDPSV-------SPVHGEDGKLDV--ESVQAALRNFQQELRDAQRERDDAQAQVMSLKRQLSELQAGQEKSSARAQQLKHSIKDLEQGKRDLAERLEAVQASLSLQEEAGRRGDRERRVMEEELSRLKNGLQASDAGARALQERLERVEQSEAGLGAECRRLKECWDAAEARASRLELSHRTLEGELQRSQIRSAELEAELSSLQERMSSLRRELGESEECTALLKVEGERAATALARAELQEAQLREKLQSTAAALKESCSSAGAAKEQTAQLQSTLTASELEGRTLQERLEKMREALSDSKKQNYALSERLQNLQRTQEEYELRVSELDKHTHSLQEALRQREQVVEEARADCTVLQKDRVELQERVTNLQTSLHSLQDDRTQLDKILTRLGKDKAALKRTLQK--------------------------------------------SQLSHLEQAHSQRLLEVTARHRQELELETERLRGSQQQAERALEAREKAHRQRVRCLEEQVCTLKEQLEQEMRRR 1922
BLAST of Rootletin vs. Ensembl Cavefish
Match: si:dkey-230p4.1 (trichohyalin-like [Source:NCBI gene;Acc:103036019]) HSP 1 Score: 83.1889 bits (204), Expect = 3.786e-15 Identity = 60/176 (34.09%), Postives = 97/176 (55.11%), Query Frame = 3
Query: 441 LESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCL-----------QTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELT 935
LESTLI+LEEEQ+++ L E N L+ QL+Q+ ++NQ L D+ KL+ +W R+ EE QRE W+ E+ +++E + L L ++ RRQ +K +T++++ L+ EF+R + L + S+ + M E Q+ ST D+ +L QLR + ELT
Sbjct: 128 LESTLIRLEEEQQRSVALVEVNALLRSQLSQSAEANQTLQDDLRKLTADWSRAVEEAGQREIDWQKEKEVLVGCVSAERARLMMLWGKAVSLRRQCHTVKTATDKDLWELRAEFSRLSSTLFSMGFPPVVPRPSVLSSSTGPMTLEELNQETSTTNHLDDQESLTQQLRSRIEELT 303
BLAST of Rootletin vs. Ensembl Cavefish
Match: si:dkey-230p4.1 (trichohyalin-like [Source:NCBI gene;Acc:103036019]) HSP 1 Score: 83.1889 bits (204), Expect = 3.786e-15 Identity = 60/176 (34.09%), Postives = 97/176 (55.11%), Query Frame = 3
Query: 441 LESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCL-----------QTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELT 935
LESTLI+LEEEQ+++ L E N L+ QL+Q+ ++NQ L D+ KL+ +W R+ EE QRE W+ E+ +++E + L L ++ RRQ +K +T++++ L+ EF+R + L + S+ + M E Q+ ST D+ +L QLR + ELT
Sbjct: 128 LESTLIRLEEEQQRSVALVEVNALLRSQLSQSAEANQTLQDDLRKLTADWSRAVEEAGQREIDWQKEKEVLVGCVSAERARLMMLWGKAVSLRRQCHTVKTATDKDLWELRAEFSRLSSTLFSMGFPPVVPRPSVLSSSTGPMTLEELNQETSTTNHLDDQESLTQQLRSRIEELT 303
BLAST of Rootletin vs. Ensembl Cavefish
Match: ENSAMXT00000053516.1 (pep primary_assembly:Astyanax_mexicanus-2.0:22:18273005:18276973:-1 gene:ENSAMXG00000035105.1 transcript:ENSAMXT00000053516.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 68.9366 bits (167), Expect = 1.121e-12 Identity = 47/160 (29.38%), Postives = 83/160 (51.88%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEE 644
+ L Q N++LR+RLEEE Y+RKL +Q+ +++ +L K+Q ++T + + R+ + Y+ + + +++ L+ N L+EQL QA+ +N+ L++DI +L+ +W ++REELEQRE WR EE
Sbjct: 17 RSLAQQNVDLRRRLEEEQAAYKRKLTAYQEG------------------QQRQAQLVQKLQAKVFQTFIKHLQHQKPHSHQQRNCSLYETQSVCVCVCV-----SRSSSLSAVNAMLREQLEQASLANEALSQDIRRLTADWTKAREELEQREADWRREE 153
BLAST of Rootletin vs. Ensembl Nematostella
Match: EDO42343 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1K9]) HSP 1 Score: 410.609 bits (1054), Expect = 2.833e-126 Identity = 255/558 (45.70%), Postives = 393/558 (70.43%), Query Frame = 3
Query: 4332 DINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIF 6005
+I+PE VR+ALR+F Q + +AER+RD+A +A +L+ L E EE+ + R+Q +Q++L ++EE+++G DGRLSSAQTALM+QEETIRR ER+RKA+ EK++ D + A E ++RQL D++ + +++ ++E + + IEN E+R+T+++L KRS+EGD++R++M ++ E E L +R++ +LK Q+LE ++ SLQLT+DR+++ALAKTEEE K+K+ +L+MSL+ + + LQ +I QLQ+AL +SE DR++++ERLE+ K A EAK +NN+L +R+Q ++++ D++ RR ELEG RQ ++ Q++EAE+EL + KL EK LQER+ QR++A AE EK+E+ + H +LEKD+ AL+ +L K+ER+KL+T+E ++ +R LD+ E EN EL RQ+ ILQ QLAE EQ+H L+E+ SRHRQE+ ME + R S Q+E+T RERAH+QRVKGLEEQ++TLKDQL E+Q++ Y TR + + D DLR L+ SL VA+D + +L+ E+++L+ES+
Sbjct: 4 EIDPETVRVALRDFAQSMKEAERERDEAVTRANNLQRALAELEEERTRMDQRMQSIQKSLGESEEERRGADGRLSSAQTALMLQEETIRRLERERKALNEKITALDSSLAQAEGDRRQLRDKVANLQQSESKSDQEKEAMRAQIENTESRLTKVELKKRSVEGDIERLRMLNSENEAEKIALQERIDQMLKSQQELESRATSLQLTVDRLTLALAKTEEEEMAFKNKVTELSMSLNDSNSTSQSLQERIQQLQRALTNSEHDRKIMQERLEALKNAQQEAKGRNNMLQDRMQQMKNEQADADVRRMELEGQIRQLQQILRQQKEAEEELVARIGKLQEEKRELQERLAKFQRSVAAAEQEKRELERAHVRLEKDKKALRNTLDKIEREKLETDETNTRLRDDRERLDRSSANFEHENQELHRQIQILQQQLAETEQSHARKLVEVTSRHRQEIEMEGDRARQSQGQLEKTQMARERAHKQRVKGLEEQVATLKDQLAKELQKK--QSYL--TRTAQKSDEIKDLRSKLEDSLNIVARDTIYEPTVLDRESQKLEESLV 557
BLAST of Rootletin vs. Ensembl Medaka
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:NCBI gene;Acc:101159974]) HSP 1 Score: 570.081 bits (1468), Expect = 1.555e-171 Identity = 441/1193 (36.97%), Postives = 701/1193 (58.76%), Query Frame = 3
Query: 2340 SNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTY---ATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGL--NRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAH--CHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTG 5897
S ++ + + LTK+K LVVQ+TA EREN +E + + S+RE LE +L+DAQQ ++Q E+ +EQLE ENQ L V+ E GE N+ QL Q +++ K A+ Q + + ++ + + RH + E E + + E +D +Q +EK+E+ + +Q ERD SLL AE EKQQ+LSL E EK + EK+ Q +L ++ E +R +E++ +EQ++ + L+SEL+ RSQ ED HE ++ SLN DL D + L AEE R+ LRR+ +E R+L + +EA E+ +RE EL+R LC+ RE++ + SN LR+ ++ ETERI + RQ + +Q++ V +E L +S++EV ELR+ L++VE++RL+ +RELQELRR +K LD +R + +E+ +LQTR++ +E++E+E +E F LKQK+ ET+ +RE+++++L+ Q+ +V+ E+ +S +R+ ++ ++ +R +KL D +L + + LS A+GR++A E+ + R E +K+++EF+L+S+ S+L R +G + R+R+ DNT A + +G G+P+P P+ P +++P+ +R LR+F+Q+L DA R RDDA Q +L+ L+EQ+ + E AENR QLQ +L + +E K+G+D RL+S Q L QE+ ++R +R+R+A+A+++ +R+ + E EK+ +++LNK R +ARLE E K+ ++A+E AE R T+++L +RSLEG+LQR+K+ L D+E E++ +R + LL+Q+ + E + LQ +DR+S AL K +E + K K+ L+ SL + Q +++ LQK L EQD+RLL+E+++ ++ ++ EAK L ER+Q +Q +L SE R ELE N Q + QR + E QK +E+ ++ER+ ++QR +AL E EK++ + +LEKD+ AL+ +L KVERQKLK EE + + E++ LD+ L +E E E Q+Q+ +LQ LAE EQAH C L+ R R E E E LR S +ERTL RERAHR RVKGLEEQ+STLK+QL EM+RR ST + STG
Sbjct: 431 SEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATGE-------------GKTNAENQLA----QAERD-KQALTQALSAAQLEAQQASLV----RHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRAD-----LEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGI----GASGRARSTP-----------------------DNTMGSGAVSPEGGGTPLP---------------PAQ----P-ELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEVRALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQVTLAEMEQAHSGCEGLL----RQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPP--LPST-----FTSTG 1538 HSP 2 Score: 130.568 bits (327), Expect = 1.416e-29 Identity = 66/170 (38.82%), Postives = 108/170 (63.53%), Query Frame = 3
Query: 474 QKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHR 983
Q++++ L+ N L+EQL QA +N+ L+RDI +L+ +W ++REELEQ+E WR EE SF+ Y +SEH L R + FRR E+K+S ER++++++ E R + +Q +C+ LS ++S E+ L+ E+E + LE QLR + +E + Q+R + E +EL R
Sbjct: 62 QQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLR 231
BLAST of Rootletin vs. Ensembl Medaka
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:NCBI gene;Acc:101159974]) HSP 1 Score: 564.303 bits (1453), Expect = 1.616e-168 Identity = 486/1345 (36.13%), Postives = 761/1345 (56.58%), Query Frame = 3
Query: 1917 ELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESN----------RHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRI-ALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTY---ATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGL--NRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAH--CHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTG 5897
EL + ++K Q + L ++++K+ + E L + + ++ I QLE E++ L +KR EQEK ++DELVR EQ L L + + +Q ++ E + +E EL L E+L L E++ Q ++L EL + E +RS ++ + + LTK+K LVVQ+TA EREN +E + + S+RE LE +L+DAQQ ++Q E+ +EQLE ENQ L V+ E D E ++A Q ERD A LT+ + RH + E E + + E +D +Q +EK+E+ + +Q ERD SLL AE EKQQ+LSL E EK + EK+ Q +L ++ E +R +E++ +EQ++ + L+SEL+ RSQ ED HE ++ SLN DL D + L AEE R+ LRR+ +E R+L + +EA E+ +RE EL+R LC+ RE++ + SN LR+ ++ ETERI + RQ + +Q++ V +E L +S++EV ELR+ L++VE++RL+ +RELQELRR +K LD +R + +E+ +LQTR++ +E++E+E +E F LKQK+ ET+ L+ Q+ +V+ E+ +S +R+ ++ ++ +R +KL D A N +R A + GR++A E+ + R E +K+++EF+L+S+ S+L R +G SR S DNT A + +G G+P+P P+ P +++P+ +R LR+F+Q+L DA R RDDA Q +L+ L+EQ+ + E AENR QLQ +L + +E K+G+D RL+S Q L QE+ ++R +R+R+A+A+++ +R+ + E EK+ +++LNK R +ARLE E K+ ++A+E AE R T+++L +RSLEG+LQR+K+ L D+E E++ +R + LL+Q+ + E + LQ +DR+S AL K +E + K K+ L+ SL + Q +++ LQK L EQD+RLL+E+++ ++ ++ EAK L ER+Q +Q +L SE R ELE N Q + QR + E QK +E+ ++ER+ ++QR +AL E EK++ + +LEKD+ AL+ +L KVERQKLK EE + + E++ LD+ L +E E E Q+Q+ +LQ LAE EQAH C L+ R R E E E LR S +ERTL RERAHR RVKGLEEQ+STLK+QL EM+RR ST + STG
Sbjct: 431 ELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVA----QVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETAT-------------------------------DAENQLA-QAERDKQA-----LTQALSAAQLEAQQASLVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRAD-----LEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQT--------LSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDE------------AKNLSVRAQAAQESAGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGI---GASGRASRGRSPGGRSTP----------------DNTMGSGAVSPEGGGTPLP---------------PAQ----P-ELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEVRALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQVTLAEMEQAHSGCEGLL----RQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPP--LPST-----FTSTG 1659 HSP 2 Score: 119.013 bits (297), Expect = 3.800e-26 Identity = 83/194 (42.78%), Postives = 116/194 (59.79%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKAS 746
+ L Q N+ELR+RL+EE YRRKL +Q+ QQ+QAQLVQ+LQ KVLQYK++C +LE K+Q E K S + E EQ++++ L+ N L+EQL QA +N+ L+RDI +L+ +W ++REELEQ+E WR EE SF+ Y +SEH L R + FRR E+K+S
Sbjct: 122 RSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSDSSSSLEEALLHLEE----------------EQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSS 299
BLAST of Rootletin vs. Ensembl Medaka
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:NCBI gene;Acc:101159974]) HSP 1 Score: 558.91 bits (1439), Expect = 1.479e-166 Identity = 476/1329 (35.82%), Postives = 758/1329 (57.04%), Query Frame = 3
Query: 1917 ELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGL--NRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTG 5897
EL + ++K Q + L ++++K+ + E L + + ++ I QLE E++ L +KR EQEK ++DELVR EQ L L + + +Q ++ E + +E EL L E+L L E++ Q ++L EL + E +RS ++ + + LTK+K LVVQ+TA EREN +E + + S+RE LE +L+DAQQ ++Q E+ +EQLE ENQ L V+ E GE N+ QL Q +++ K A+ Q + + ++ + + RH + E E + + E +D +Q +EK+E+ + +Q ERD SLL AE EKQQ+LSL E EK + EK+ Q +L ++ E +R +E++ +EQ++ + L+SEL+ RSQ ED HE ++ SLN DL D ALKE+ L AEE R+ LRR+ +E R+L + +EA E+ +RE EL+R LC+ RE++ + SN LR+ ++ ETERI + RQ + +Q++ V +E L +S++EV ELR+ L++VE++RL+ +RELQELRR +K LD +R + +E+ +LQTR++ +E++E+E +E F LKQK+ ET+ L+ Q+ +V+ E+ +S +R+ ++ ++ +R +KL D +L + + LS A+GR++A E+ + R E +K+++EF+L+S+ S+L R +G + G+ SP + SP S R P+ P +++P+ +R LR+F+Q+L DA R RDDA Q +L+ L+EQ+ + E AENR QLQ +L + +E K+G+D RL+S Q L QE+ ++R +R+R+A+A+++ +R+ + E EK+ +++LNK R +ARLE E K+ ++A+E AE R T+++L +RSLEG+LQR+K+ L D+E E++ +R + LL+Q+ + E + LQ +DR+S AL K +E + K K+ L+ SL + Q +++ LQK L EQD+RLL+E+++ ++ ++ EAK L ER+Q +Q +L SE R ELE N Q + QR + E QK +E+ ++ER+ ++QR +AL E EK++ + +LEKD+ AL+ +L KVERQKLK EE + + E++ LD+ L +E E E Q+Q+ +LQ A AH L+ R R E E E LR S +ERTL RERAHR RVKGLEEQ+ QL EM+RR ST + STG
Sbjct: 438 ELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVA----QVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATGE-------------GKTNAENQLA----QAERD-KQALTQALSAAQLEAQQASLV----RHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQT--------LSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGI--------------------------------------GASGRASRGR-SPGGSSASPGSISRGTPLPPA----QP-ELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEVRALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQV-TASNSSAHSTGLL----RQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVWFHDSQLQQEMKRRQPP--LPST-----FTSTG 1676 HSP 2 Score: 119.398 bits (298), Expect = 3.422e-26 Identity = 83/195 (42.56%), Postives = 117/195 (60.00%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKAST 749
+ L Q N+ELR+RL+EE YRRKL +Q+ QQ+QAQLVQ+LQ KVLQYK++C +LE K+Q E K S + E EQ++++ L+ N L+EQL QA +N+ L+RDI +L+ +W ++REELEQ+E WR EE SF+ Y +SEH L R + FRR E+K+S+
Sbjct: 132 RSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSDSSSSLEEALLHLEE----------------EQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSS 310
BLAST of Rootletin vs. Ensembl Medaka
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:NCBI gene;Acc:101159974]) HSP 1 Score: 549.28 bits (1414), Expect = 1.666e-163 Identity = 465/1332 (34.91%), Postives = 743/1332 (55.78%), Query Frame = 3
Query: 1917 ELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTY---ATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAH--CHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTG 5897
EL + ++K Q + L ++++K+ + E L + + ++ I QLE E++ L +KR EQEK ++DELVR EQ L L + + +Q ++ E + +E EL L E+L L E++ Q ++L EL + E +RS ++ + + LTK+K LVVQ+TA EREN +E + + S+RE LE +L+DAQQ ++Q E+ +EQLE ENQ L V+ E GE N+ QL Q +++ K A+ Q + + ++ + + RH + E E + + E +D +Q +EK+E+ + +Q ERD SLL AE EKQQ+LSL E EK + EK+ Q +L ++ E +R +E++ +EQ++ + L+SEL+ RSQ ED HE ++ SLN DL D ALKE+ L AEE R+ LRR+ +E R+L + +EA E+ +RE EL+R LC+ RE++ + SN LR+ ++ ETERI + RQ + +Q++ V +E L +S++EV ELR+ L++VE++RL+ +RELQELRR +K LD +R + +E+ +LQTR++ +E++E+E +E F LKQK+ ET+ L+ Q+ +V+ E+ +S +R+ ++ ++ +R +KL D +L + + LS A+GR++A E+ + R E +K+++EF+L+S+ S+L R +G DNT A + +G G+P+P P+ P +++P+ +R LR+F+Q+L DA R RDDA Q +L+ L+EQ+ + E AENR QLQ +L + +E K+G+D RL+S Q L QE+ ++R +R+R+A+A+++ +R+ + E EK+ +++LNK R +ARLE E K+ ++A+E AE R T+++L +RSLEG+LQR+K+ L D+E E++ +R + LL+Q+ + E + LQ +DR+S AL K +E + K K+ L+ SL + Q +++ LQK L EQD+RLL+ + G A+ Q+++ QK +E+ ++ER+ ++QR +AL E EK++ + +LEKD+ AL+ +L KVERQKLK EE + + E++ LD+ L +E E E Q+Q+ +LQ + EQAH C L+ R R E E E LR S +ERTL RERAHR RVKGLEEQ+STLK+QL EM+RR ST + STG
Sbjct: 452 ELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVA----QVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATGE-------------GKTNAENQLA----QAERD-KQALTQALSAAQLEAQQASLV----RHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQT--------LSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGSTP------------------------------DNTMGSGAVSPEGGGTPLP----PA---------------QP-ELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQVSGSTRGGWRSLAETQHSI-----------------------------------------------QKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQVTM---EQAHSGCEGLL----RQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPP--LPST-----FTSTG 1638 HSP 2 Score: 118.627 bits (296), Expect = 5.855e-26 Identity = 83/194 (42.78%), Postives = 116/194 (59.79%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKAS 746
+ L Q N+ELR+RL+EE YRRKL +Q+ QQ+QAQLVQ+LQ KVLQYK++C +LE K+Q E K S + E EQ++++ L+ N L+EQL QA +N+ L+RDI +L+ +W ++REELEQ+E WR EE SF+ Y +SEH L R + FRR E+K+S
Sbjct: 136 RSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSDSSSSLEEALLHLEE----------------EQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSS 313
BLAST of Rootletin vs. Ensembl Medaka
Match: CROCC (ciliary rootlet coiled-coil, rootletin [Source:NCBI gene;Acc:101159974]) HSP 1 Score: 544.658 bits (1402), Expect = 1.101e-161 Identity = 422/1151 (36.66%), Postives = 676/1151 (58.73%), Query Frame = 3
Query: 2463 SLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTY---ATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDR-DDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAH--CHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTG 5897
+ S+RE LE +L+DAQQ ++Q E+ +EQLE ENQ L V+ E EL RLR + + + + ++ L E + A ++ H E+VERL E RH + E E + + E +D +Q +EK+E+ + +Q ERD SLL AE EKQQ+LSL E EK + EK+ Q +L ++ E +R +E++ +EQ++ + L+SEL+ RSQ ED HE ++ SLN DL D + L AEE R+ LRR+ +E R+L + +EA E+ +RE EL+R LC+ RE++ + SN LR+ ++ ETERI + RQ + +Q++ V +E L +S++EV ELR+ L++VE++RL+ +RELQELRR +K LD +R + +E+ +LQTR++ +E++E+E +E F LKQK+ ET+ +RE+++++L+ Q+ +V+ E+ +S +R+ ++ ++ +R +KL D +L + + LS A+GR++A E+ + R E +K+++EF+L+S+ S+L R +G + R+R+ DNT A + +G G+P+P P+ P +++P+ +R LR+F+Q+L DA R R +D F ++L+EQ+ + E AENR QLQ +L + +E K+G+D RL+S Q L QE+ ++R +R+R+A+A+++ +R+ + E EK+ +++LNK R +ARLE E K+ ++A+E AE R T+++L +RSLEG+LQR+K+ L D+E E++ +R + LL+Q+ + E + LQ +DR+S AL K +E + K K+ L+ SL + Q +++ LQK L EQD+RLL+E+++ ++ ++ EAK L ER+Q +Q +L SE R ELE + + + QR + E QK +E+ ++ER+ ++QR +AL E EK++ + +LEKD+ AL+ +L KVERQKLK EE + + E++ LD+ L +E E E Q+Q+ +LQ QAH C L+ R R E E E LR S +ERTL RERAHR RVKGLEEQ+STLK+QL EM+RR ST + STG
Sbjct: 573 ACRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRAD-----LEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGI----GASGRARSTP-----------------------DNTMGSGAVSPEGGGTPLP---------------PAQ----P-ELDPDTLRSGLRDFLQELRDAHRQRVNDCFKHETDT-AQLEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQ-----VTQAHSGCEGLL----RQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPP--LPST-----FTSTG 1654 HSP 2 Score: 168.318 bits (425), Expect = 4.028e-41 Identity = 111/273 (40.66%), Postives = 166/273 (60.81%), Query Frame = 3
Query: 165 KGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHR 983
+ L Q N+ELR+RL+EE YRRKL +Q+ QQ+QAQLVQ+LQ KVLQYK++C +LE K+Q E K S L+ LEEEQ++++ L+ N L+EQL QA +N+ L+RDI +L+ +W ++REELEQ+E WR EE SF+ Y +SEH L R + FRR E+K+S ER++++++ E R + +Q +C+ LS ++S E+ L+ E+E + LE QLR + +E + Q+R + E +EL R
Sbjct: 122 RSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKC------------------------SALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLR 370
BLAST of Rootletin vs. Planmine SMEST
Match: SMESG000071494.1 (SMESG000071494.1) HSP 1 Score: 3619.71 bits (9385), Expect = 0.000e+0 Identity = 1980/2008 (98.61%), Postives = 1983/2008 (98.75%), Query Frame = 3
Query: 84 MSEIDYSAIVRRRXXXXXXXXXXXXXXKGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKRIEMSKKSAAPNNSNRRLTAG 6107
MSEIDYSAIVRRRIGDIDGDSSEDSSSKGLLQVNLELRKRLEEEHNEYRRKLIVFQDSQQKQAQLVQRLQKKVLQYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVRKNN K DATYAAVQSAINKKSLQIQELKSKLNSSKESCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR LEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIEQLEINTERLEEELSELNREKLDLTEQIGCLQRQKSALAEELLQCRRELERSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLN+GIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNRKLKEAEEAKEHLKREINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTRVARDEEKEDEARRENFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKSPVRGRSVSPLRSRPVSPTKGFDNTYATTTDGKGSPIPRTGSPSSK+RQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDRRLLEERLESSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKR+EMSKKSAAP NSNRRLTAG
Sbjct: 1 MSEIDYSAIVRRRIGDIDGDSSEDSSSKGLLQVNLELRKRLEEEHNEYRRKLIVFQDSQQKQAQLVQRLQKKVLQYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVRKNNHQ---------------LKHRHLPDATYAAVQSAINKKSLQIQELKSKLNSSKESCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRCLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIEQLEINTERLEEELSELNREKLDLTEQIGCLQRQKSALAEELLQCRRELERSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNNGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNRKLKEAEEAKEHLKREINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTRVARDEEKEDEARRENFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKSPVRGRSVSPLRSRPVSPTKGFDNTYATTTDGKGSPIPRTGSPSSKQRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDRRLLEERLESSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKRMEMSKKSAAPTNSNRRLTAG 1993
BLAST of Rootletin vs. Planmine SMEST
Match: SMESG000071494.1 (SMESG000071494.1) HSP 1 Score: 3618.17 bits (9381), Expect = 0.000e+0 Identity = 1983/2008 (98.75%), Postives = 1987/2008 (98.95%), Query Frame = 3
Query: 84 MSEIDYSAIVRRRXXXXXXXXXXXXXXKGLLQVNLELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPPFADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKRIEMSKKSAAPNNSNRRLTAG 6107
MSEIDYSAIVRRRIGDIDGDSSEDSSSKGLLQVNLELRKRLEEEHNEYRRKLIVFQDSQQKQAQLVQRLQKKVLQYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVRKNNRSP+ K DATYAAVQSAINKKSLQIQELKSKLNSSKESCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKR LEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIEQLEINTERLEEELSELNREKLDLTEQIGCLQRQKSALAEELLQCRRELERSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLN+GIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNRKLKEAEEAKEHLKREINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTRVARDEEKEDEARRENFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKSPVRGRSVSPLRSRPVSPTKGFDNTYATTTDGKGSPIPRTGSPSSK+RQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDRRLLEERLESSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKR+EMSKKSAAP NSNRRLTAG
Sbjct: 1 MSEIDYSAIVRRRIGDIDGDSSEDSSSKGLLQVNLELRKRLEEEHNEYRRKLIVFQDSQQKQAQLVQRLQKKVLQYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGSRSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNGDTAGEINMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFDAEDTGFETVRKNNRSPNQ------------LKHRHLPDATYAAVQSAINKKSLQIQELKSKLNSSKESCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRCLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIEQLEINTERLEEELSELNREKLDLTEQIGCLQRQKSALAEELLQCRRELERSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNNGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNRKLKEAEEAKEHLKREINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTRVARDEEKEDEARRENFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKSPVRGRSVSPLRSRPVSPTKGFDNTYATTTDGKGSPIPRTGSPSSKQRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDRRLLEERLESSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQYDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCGSRDQSPTRQKRMEMSKKSAAPTNSNRRLTAG 1996
BLAST of Rootletin vs. Planmine SMEST
Match: SMESG000081227.1 (SMESG000081227.1) HSP 1 Score: 1043.49 bits (2697), Expect = 0.000e+0 Identity = 782/1978 (39.53%), Postives = 1218/1978 (61.58%), Query Frame = 3
Query: 186 LELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYKEKCTELESKMQFGDYETRKTKYGSPTNNGYGS-RSENPYDNLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNG---DTAGEI-NMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD----AEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPP------------------------FADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQ--YDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCG 6014
++LRK+L+++H EY+RKL D ++ K EL ++Q ++T + + +S N+DLES + +L+EEQ K+ L + L++QL +AT +NQ LT D+ +LSEE + ELEQ++ W+++E +F+ +L E + +++L +NF+RQF ++K+S ER+++ L+ + + L+ ACSNL N + S+ Q + E + LEN L + E +T+Y+ ++ L + + ++ L + +K++ LT L +++NG D I N +D ATR LI DT+++HQALR+I+Q+V+ND D D +D + + + S+ N + T P AD+T AAV+SA++K++LQ+Q+L SK+NS+K + + K+Q+DD+ENERR LE Q + +R E +S R+ DD RE +R ++ +++ ++ +L+K R + ++ + ++E+LK+ N+ELQR +E L D +D ++LERQ KE ER +SI+ E K SR KE++ + KE++N+ LEKEVL QE + D+L++ E+ KAE E++ NK +T E L + +L S L + +E I Q E E+ L EK+ LEQEK G+++EL+R E E L+ K Q ++ +E + E L++ELS+LN EK L LQR+++AL+ ELLQ RR+ ER + +V K+KE L KEK +L+ Q+T E E R+ ++ I L ++EQL+ +LY++QQ I QLE + E LE Q+L K+ + E RL+ EF +E +K+ ++E L + + +KE+ ++ E+++ ED+ERL E R+ +EE+ +F E DD+ ++K+E+AQEI+TIQRERDD LL AE KQ +++LLEQEK ++ EK+ + L++ ES+L+R + E R +QD+ I + +LK FR QFE +H+ E+K+L K+L K Q ++E +L + LKIAEETRDNLR+++ E K++E E+ +E L+RE+ + KR E ++E E + SN+ LR+K+K E ++ID+ RQ+ D + K+++ +EQ ++EV + R NL+D EKAR+DV+REL+ELR +K+LDTERGKL EV+ LQ RVA DE KE EARRENFGLKQKV+E+EA++E + K L + R +LE +L SK++ + +DY+ L +++ +L+ + + EL LS E R+S LE+ I ++ +K E +LA+IHS LR+LIGFRQ+ T+R+R+ S + F+ + G+ + + S P + D++PEAVR+ALR+ Q +V ER+RDD +A++ + E Q + A R+ QLQ+ + EEDKKGID RLSSAQTALMMQEETI RNER+RK++ +K+ +R A +E+EK+ L++R++K R ++ EEE + KA+++AENR TQL+L +R LEG+LQR+KM DKETE +VL DRV++L KQI DLE K+QSLQLTIDR+S+ LA+TEEE + KDK+ QLN+SLS H L+ +Q+K+ QLQ L SSE DRR+L+ERLES K L EAK QN+ L++R++ Q +L +SE+R+ E+EG ++ + ++ QRQE EQEL K + EKN L ER+NS+ +LA E+E+++I K H K EK++++LKK+L K+E++K ++EE ++++ +RSE D+ RRLEDEN L RQV LQNQLAEAEQ H +ME+ SR + EM E++ L+++ E+TL+ RER+HRQ+VK LEEQI+ LKDQL+ E+++R + KSN+ + D++ +L+ + ++K D +L E E RR+ + + G
Sbjct: 184 IQLRKQLDDDHREYKRKLAAQHD------------------HQIKQAELIKRLQ-QKFQTTTSDLNRRIDIAENHLKSAETDKNMDLESVVNELKEEQSKSMSLLQMTALLRDQLDEATNANQGLTEDVQRLSEENSFLKSELEQKDMDWKDDEATFSRFLIGEQNRIKSLWNAVVNFKRQFNDIKSSNERDLHQLRVNIEQSTQSLRLACSNLDTNHVNRLSQLQFQLDTEKNNCSQLENSLSKMKEEFRKTETQYQKQIESLNEQFVHVNRSNESLTKELADKEKVLTTLHKLRNGMNGDLDNSIYNNDDSATRQLIIDTDAVHQALRDIAQLVMNDVDQVDDSPWMQDDYLDESKTTGQLKPTLSINSLHSNRSVSPTRPSKRSHFNSGSLDRNRVYRNKSPVSIADSTCAAVRSALHKRALQVQDLTSKINSAKNNHNSVKKQLDDSENERRNLEKQFIELRSEYDSNHRELDDIQRECERMRHTISMLEEDKLKLDKSRMNSYEECKSYKQDMEKLKTLNYELQRDKENLTDNCQDLDRNLERQTKEVERLTKSIENSESKSSRLKEEISSLKESLNRALLEKEVLEQENSVLTDSLNKTEVLKAEYEVKANKFKTEEARLRDKFHQLESMNHFLVKENSEFKTIIDQTESEKHRLSGEKKALEQEKYGIREELMRVENEKEELNQMKDGLHQSMQNVEKSYENLQDELSKLNYEKGQLETANVTLQRERNALSTELLQARRDAERLSAAVQKSMKDKEVLAKEKIDLISQLTTFEHELRENADAIYVLQEEKEQLDGSLYESQQNISQLEAKIEALESNLQQLERKRSIIFDEKQRLQHEFDVELQKLSKAKESLQIKLNESEKEWNKILRSEKEAREEDIERLIAEREELRNEFMKYKEEIKLQFQEEIDDLRHRLNRDKEELAQEIITIQRERDDCLLEAEMGKQHAINLLEQEKMSMYEKISQLTDLLRTSESDLERTRNECEIRAQQDQNTIQTFNGDLKKFRLQFEQIHIDHDREIKNLQCAYKELENQKYQDMRENEELKSLLKIAEETRDNLRQDLSESMGKIRETEDQRERLRRELMDEKRNYFEVKKELETIQLSNKELREKLKTSEMQKIDIQRQLQDLQNKLSILEEQKQVLQKEVKDTRYNLQDTEKARVDVRRELEELRSQVKKLDTERGKLVNEVSGLQVRVATDENKEVEARRENFGLKQKVIESEATKEELSKQLYQSLRKNEELEEILNSKEKTTKQYYDEVLKDYKSLEESKLNLENQLEITNHNFEELNSTLSTKENRISLLENQINILDQKRKGAENRLATIHSHLRKLIGFRQQICQTLRNRSPSPGRRSRSVSPEKGRVSSRNSVEFNFSEDRKRVGRFGDVNQYSE--------SVIPGS------DLDPEAVRIALRDITQHIVSIERERDDMEIKAKNSALKSDETSVQLDQALQRINQLQKNFGEVEEDKKGIDSRLSSAQTALMMQEETIHRNERERKSLIDKVGKLERQLAHVETEKKYLQERVDKMRTGESYHEEERRGLIKAVDDAENRCTQLELLRRGLEGELQRLKMGFNDKETENQVLRDRVDALTKQISDLESKAQSLQLTIDRLSMNLARTEEEENTQKDKVHQLNLSLSEHQGNLQSMQDKLVQLQSTLASSEHDRRVLQERLESVKHNLSEAKSQNHTLVDRMKATQQELMESESRKHEIEGEIKRLSQILSQRQEIEQELQSKIHRSAMEKNLLTERLNSLTASLAGLESERRDIEKVHIKTEKEKNSLKKTLDKLEKEKNRSEESINQSLLKRSEFDEFTRRLEDENEVLHRQVQKLQNQLAEAEQCHAQRIMELTSRIKNEMLSEIDRLKSALNSTEKTLQFRERSHRQKVKSLEEQINILKDQLSQELKKR-----ELNLNKSNRGLKNDICDIKSILEKNSNNISK-TSFDNLLYEPEIRRMKDILSESG 2122
BLAST of Rootletin vs. Planmine SMEST
Match: SMESG000081227.1 (SMESG000081227.1) HSP 1 Score: 1039.25 bits (2686), Expect = 0.000e+0 Identity = 795/1991 (39.93%), Postives = 1236/1991 (62.08%), Query Frame = 3
Query: 186 LELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYK------EKCTELESKMQFGDYETRKTKYGSPTN--NGYGSRSENPYD------NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNG---DTAGEI-NMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD----AEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPP------------------------FADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQ--YDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCG 6014
++LRK+L+++H EY+RKL D Q KQA+L++RLQ+K+ Q + EK + SK++ + K + + T+ N +EN N+DLES + +L+EEQ K+ L + L++QL +AT +NQ LT D+ +LSEE + ELEQ++ W+++E +F+ +L E + +++L +NF+RQF ++K+S ER+++ L+ + + L+ ACSNL N + S+ Q + E + LEN L + E +T+Y+ ++ L + + ++ L + +K++ LT L +++NG D I N +D ATR LI DT+++HQALR+I+Q+V+ND D D +D + + + S+ N + T P AD+T AAV+SA++K++LQ+Q+L SK+NS+K + + K+Q+DD+ENERR LE Q + +R E +S R+ DD RE +R ++ +++ ++ +L+K R + ++ + ++E+LK+ N+ELQR +E L D +D ++LERQ KE ER +SI+ E K SR KE++ + KE++N+ LEKEVL QE + D+L++ E+ KAE E++ NK +T E L + +L S L + +E I Q E E+ L EK+ LEQEK G+++EL+R E E L+ K Q ++ +E + E L++ELS+LN EK L LQR+++AL+ ELLQ RR+ ER + +V K+KE L KEK +L+ Q+T E E R+ ++ I L ++EQL+ +LY++QQ I QLE + E LE Q+L K+ + E RL+ EF +E +K+ ++E L + + +KE+ ++ E+++ ED+ERL E R+ +EE+ +F E DD+ ++K+E+AQEI+TIQRERDD LL AE KQ +++LLEQEK ++ EK+ + L++ ES+L+R + E R +QD+ I + +LK FR QFE +H+ E+K+L K+L K Q ++E +L + LKIAEETRDNLR+++ E K++E E+ +E L+RE+ + KR E ++E E + SN+ LR+K+K E ++ID+ RQ+ D + K+++ +EQ ++EV + R NL+D EKAR+DV+REL+ELR +K+LDTERGKL EV+ LQ RVA DE KE EARRENFGLKQKV+E+EA++E + K L + R +LE +L SK++ + +DY+ L +++ +L+ + + EL LS E R+S LE+ I ++ +K E +LA+IHS LR+LIGFRQ+ T+R+R+ S + F+ + G+ + + S P + D++PEAVR+ALR+ Q +V ER+RDD +A++ + E Q + A R+ QLQ+ + EEDKKGID RLSSAQTALMMQEETI RNER+RK++ +K+ +R A +E+EK+ L++R++K R ++ EEE + KA+++AENR TQL+L +R LEG+LQR+KM DKETE +VL DRV++L KQI DLE K+QSLQLTIDR+S+ LA+TEEE + KDK+ QLN+SLS H L+ +Q+K+ QLQ L SSE DRR+L+ERLES K L EAK QN+ L++R++ Q +L +SE+R+ E+EG ++ + ++ QRQE EQEL K + EKN L ER+NS+ +LA E+E+++I K H K EK++++LKK+L K+E++K ++EE ++++ +RSE D+ RRLEDEN L RQV LQNQLAEAEQ H +ME+ SR + EM E++ L+++ E+TL+ RER+HRQ+VK LEEQI+ LKDQL+ E+++R + KSN+ + D++ +L+ + ++K D +L E E RR+ + + G
Sbjct: 184 IQLRKQLDDDHREYKRKLAAQHDHQIKQAELIKRLQQKLEQLEGRNGLVEKLNQQNSKLR-SEISRLKNHFQTTTSDLNRRIDIAENHLKSAETDKNMDLESVVNELKEEQSKSMSLLQMTALLRDQLDEATNANQGLTEDVQRLSEENSFLKSELEQKDMDWKDDEATFSRFLIGEQNRIKSLWNAVVNFKRQFNDIKSSNERDLHQLRVNIEQSTQSLRLACSNLDTNHVNRLSQLQFQLDTEKNNCSQLENSLSKMKEEFRKTETQYQKQIESLNEQFVHVNRSNESLTKELADKEKVLTTLHKLRNGMNGDLDNSIYNNDDSATRQLIIDTDAVHQALRDIAQLVMNDVDQVDDSPWMQDDYLDESKTTGQLKPTLSINSLHSNRSVSPTRPSKRSHFNSGSLDRNRVYRNKSPVSIADSTCAAVRSALHKRALQVQDLTSKINSAKNNHNSVKKQLDDSENERRNLEKQFIELRSEYDSNHRELDDIQRECERMRHTISMLEEDKLKLDKSRMNSYEECKSYKQDMEKLKTLNYELQRDKENLTDNCQDLDRNLERQTKEVERLTKSIENSESKSSRLKEEISSLKESLNRALLEKEVLEQENSVLTDSLNKTEVLKAEYEVKANKFKTEEARLRDKFHQLESMNHFLVKENSEFKTIIDQTESEKHRLSGEKKALEQEKYGIREELMRVENEKEELNQMKDGLHQSMQNVEKSYENLQDELSKLNYEKGQLETANVTLQRERNALSTELLQARRDAERLSAAVQKSMKDKEVLAKEKIDLISQLTTFEHELRENADAIYVLQEEKEQLDGSLYESQQNISQLEAKIEALESNLQQLERKRSIIFDEKQRLQHEFDVELQKLSKAKESLQIKLNESEKEWNKILRSEKEAREEDIERLIAEREELRNEFMKYKEEIKLQFQEEIDDLRHRLNRDKEELAQEIITIQRERDDCLLEAEMGKQHAINLLEQEKMSMYEKISQLTDLLRTSESDLERTRNECEIRAQQDQNTIQTFNGDLKKFRLQFEQIHIDHDREIKNLQCAYKELENQKYQDMRENEELKSLLKIAEETRDNLRQDLSESMGKIRETEDQRERLRRELMDEKRNYFEVKKELETIQLSNKELREKLKTSEMQKIDIQRQLQDLQNKLSILEEQKQVLQKEVKDTRYNLQDTEKARVDVRRELEELRSQVKKLDTERGKLVNEVSGLQVRVATDENKEVEARRENFGLKQKVIESEATKEELSKQLYQSLRKNEELEEILNSKEKTTKQYYDEVLKDYKSLEESKLNLENQLEITNHNFEELNSTLSTKENRISLLENQINILDQKRKGAENRLATIHSHLRKLIGFRQQICQTLRNRSPSPGRRSRSVSPEKGRVSSRNSVEFNFSEDRKRVGRFGDVNQYSE--------SVIPGS------DLDPEAVRIALRDITQHIVSIERERDDMEIKAKNSALKSDETSVQLDQALQRINQLQKNFGEVEEDKKGIDSRLSSAQTALMMQEETIHRNERERKSLIDKVGKLERQLAHVETEKKYLQERVDKMRTGESYHEEERRGLIKAVDDAENRCTQLELLRRGLEGELQRLKMGFNDKETENQVLRDRVDALTKQISDLESKAQSLQLTIDRLSMNLARTEEEENTQKDKVHQLNLSLSEHQGNLQSMQDKLVQLQSTLASSEHDRRVLQERLESVKHNLSEAKSQNHTLVDRMKATQQELMESESRKHEIEGEIKRLSQILSQRQEIEQELQSKIHRSAMEKNLLTERLNSLTASLAGLESERRDIEKVHIKTEKEKNSLKKTLDKLEKEKNRSEESINQSLLKRSEFDEFTRRLEDENEVLHRQVQKLQNQLAEAEQCHAQRIMELTSRIKNEMLSEIDRLKSALNSTEKTLQFRERSHRQKVKSLEEQINILKDQLSQELKKR-----ELNLNKSNRGLKNDICDIKSILEKNSNNISK-TSFDNLLYEPEIRRMKDILSESG 2153
BLAST of Rootletin vs. Planmine SMEST
Match: SMESG000081227.1 (SMESG000081227.1) HSP 1 Score: 1038.87 bits (2685), Expect = 0.000e+0 Identity = 795/1991 (39.93%), Postives = 1236/1991 (62.08%), Query Frame = 3
Query: 186 LELRKRLEEEHNEYRRKLIVFQDSXXXXXXXXXXXXXXXXXYK------EKCTELESKMQFGDYETRKTKYGSPTN--NGYGSRSENPYD------NLDLESTLIKLEEEQKKTNYLTEFNERLQEQLTQATQSNQNLTRDISKLSEEWQRSREELEQREKIWRNEEMSFNDYLASEHSNLQNLQRNTINFRRQFIELKASTEREINNLKNEFNRQARCLQTACSNLSCNMKSAESKQQLSTERESRDKLNLENQLREKNRELTDYQTRYECEMTELQHRVKELTVLSDKLKIQNLEKDRALTALQRIKNG---DTAGEI-NMEDKATRVLIEDTESMHQALRNISQVVLNDSDYFD----AEDTGFETVXXXXXXXXXXXXXXXXXXXXXAKTPP------------------------FADATYAAVQSAINKKSLQIQXXXXXXXXXXXXCTNFKRQIDDNENERRRLENQVLAMRDEIESLRRDKDDSFREADRFKNALNITSNERSQLEKIRSSLNDQIEQQLTEIERLKSANHELQRLREALEDEQEDTKKDLERQIKESERCQRSIDVLEDKISRFKEDVVNYKEAINKYQLEKEVLVQEKDEIGDALSRCEISKAELEIEVNKRQTIEVNLEENISKLSSEIEELNDKRAENHRTIKQLEHERSSLLNEKRFLEQEKSGLKDELVRSEQEALGLSNEKAIQRQRIXXXXXXXXXXXXXXXXXXXXXXDLTEQIGCLQRQKSAXXXXXXXXXXXXXXSNNSVNNLSKEKEYLTKEKGELVVQVTACERENRQQSEVIASLNSDREQLEATLYDAQQLIIQLETRKEQLEGENQELIVKKENLNGELVRLRQEFQLEYEKVVNSREQLNCRYQQMDKEYKMAIQQERDSHAEDVERLTKINESNRHGSENEREELIHRFNMERDDMIQNYEQEKQEMAQEIMTIQRERDDSLLMAENEKQQSLSLLEQEKCAINEKLIKTQFELQSVESELDRIKKESSNRQEQDKQNIAGLSSELKSFRSQFEDKCQNHEMEVKSLNSGIKDLTLMKDQALKEVHDLHTGLKIAEETRDNLRREIMEMNXXXXXXXXXXXXXXXXINELKRGLCEEQREKENVINSNENLRKKIKDVETERIDLHRQVTDSKQKVAVQDEQLVNSKQEVNELRNNLKDVEKARLDVKRELQELRRHIKELDTERGKLTKEVNDLQTXXXXXXXXXXXXXXXNFGLKQKVVETEASREAVRKDLANAQRHIVDLENVLQSKDRDFSSMVESSRRDYRKLADARNSLQQVYDTMVADNNELRIALSGAEGRVSALESHIARVEGAKKEIEFKLASIHSSLRRLIGFRQENVVTIRSRNNSKXXXXXXXXXXXXXXXXXXTKGFDNTYATTTDGKGSPIPRTGSPSSKRRQLSRSPSNCDFNPVDINPEAVRLALREFVQQLVDAERDRDDAFAQARSLESRLKEQEEQNEYAENRLQQLQQALCDAEEDKKGIDGRLSSAQTALMMQEETIRRNERDRKAMAEKLSNYDRTFASMESEKRQLEDRLNKFRHHDARLEEEHKQSKKAIENAENRITQLQLAKRSLEGDLQRMKMALTDKETETEVLDDRVNSLLKQIQDLEGKSQSLQLTIDRMSVALAKTEEEGSFHKDKMQQLNMSLSTHSQQLEQLQNKISQLQKALGSSEQDXXXXXXXXXSSKGALHEAKHQNNLLMERIQIIQSDLNDSENRRSELEGLNRQSNTLIVQRQEAEQELNQKYQKLNSEKNTLQERVNSMQRNLALAEAEKQEIIKNHGKLEKDRSALKKSLGKVERQKLKTEEIASKTVFERSELDQILRRLEDENCELQRQVGILQNQLAEAEQAHCHHLMEINSRHRQEMSMEMEHLRASNVQIERTLEGRERAHRQRVKGLEEQISTLKDQLTHEMQRRMDSGYYESTRKSNQ--YDSTGDLRHVLDSSLTRVAKDPQLDAMLLETEARRLDESIFMCG 6014
++LRK+L+++H EY+RKL D Q KQA+L++RLQ+K+ Q + EK + SK++ + K + + T+ N +EN N+DLES + +L+EEQ K+ L + L++QL +AT +NQ LT D+ +LSEE + ELEQ++ W+++E +F+ +L E + +++L +NF+RQF ++K+S ER+++ L+ + + L+ ACSNL N + S+ Q + E + LEN L + E +T+Y+ ++ L + + ++ L + +K++ LT L +++NG D I N +D ATR LI DT+++HQALR+I+Q+V+ND D D +D + + + S+ N + T P AD+T AAV+SA++K++LQ+Q+L SK+NS+K + + K+Q+DD+ENERR LE Q + +R E +S R+ DD RE +R ++ +++ ++ +L+K R + ++ + ++E+LK+ N+ELQR +E L D +D ++LERQ KE ER +SI+ E K SR KE++ + KE++N+ LEKEVL QE + D+L++ E+ KAE E++ NK +T E L + +L S L + +E I Q E E+ L EK+ LEQEK G+++EL+R E E L+ K Q ++ +E + E L++ELS+LN EK L LQR+++AL+ ELLQ RR+ ER + +V K+KE L KEK +L+ Q+T E E R+ ++ I L ++EQL+ +LY++QQ I QLE + E LE Q+L K+ + E RL+ EF +E +K+ ++E L + + +KE+ ++ E+++ ED+ERL E R+ +EE+ +F E DD+ ++K+E+AQEI+TIQRERDD LL AE KQ +++LLEQEK ++ EK+ + L++ ES+L+R + E R +QD+ I + +LK FR QFE +H+ E+K+L K+L K Q ++E +L + LKIAEETRDNLR+++ E K++E E+ +E L+RE+ + KR E ++E E + SN+ LR+K+K E ++ID+ RQ+ D + K+++ +EQ ++EV + R NL+D EKAR+DV+REL+ELR +K+LDTERGKL EV+ LQ RVA DE KE EARRENFGLKQKV+E+EA++E + K L + R +LE +L SK++ + +DY+ L +++ +L+ + + EL LS E R+S LE+ I ++ +K E +LA+IHS LR+LIGFRQ+ T+R+R+ S + F+ + G+ + + S P + D++PEAVR+ALR+ Q +V ER+RDD +A++ + E Q + A R+ QLQ+ + EEDKKGID RLSSAQTALMMQEETI RNER+RK++ +K+ +R A +E+EK+ L++R++K R ++ EEE + KA+++AENR TQL+L +R LEG+LQR+KM DKETE +VL DRV++L KQI DLE K+QSLQLTIDR+S+ LA+TEEE + KDK+ QLN+SLS H L+ +Q+K+ QLQ L SSE DRR+L+ERLES K L EAK QN+ L++R++ Q +L +SE+R+ E+EG ++ + ++ QRQE EQEL K + EKN L ER+NS+ +LA E+E+++I K H K EK++++LKK+L K+E++K ++EE ++++ +RSE D+ RRLEDEN L RQV LQNQLAEAEQ H +ME+ SR + EM E++ L+++ E+TL+ RER+HRQ+VK LEEQI+ LKDQL+ E+++R + KSN+ + D++ +L+ + ++K D +L E E RR+ + + G
Sbjct: 195 IQLRKQLDDDHREYKRKLAAQHDHQIKQAELIKRLQQKLEQLEGRNGLVEKLNQQNSKLR-SEISRLKNHFQTTTSDLNRRIDIAENHLKSAETDKNMDLESVVNELKEEQSKSMSLLQMTALLRDQLDEATNANQGLTEDVQRLSEENSFLKSELEQKDMDWKDDEATFSRFLIGEQNRIKSLWNAVVNFKRQFNDIKSSNERDLHQLRVNIEQSTQSLRLACSNLDTNHVNRLSQLQFQLDTEKNNCSQLENSLSKMKEEFRKTETQYQKQIESLNEQFVHVNRSNESLTKELADKEKVLTTLHKLRNGMNGDLDNSIYNNDDSATRQLIIDTDAVHQALRDIAQLVMNDVDQVDDSPWMQDDYLDESKTTGQLKPTLSINSLHSNRSVSPTRPSKRSHFNSGSLDRNRVYRNKSPVSIADSTCAAVRSALHKRALQVQDLTSKINSAKNNHNSVKKQLDDSENERRNLEKQFIELRSEYDSNHRELDDIQRECERMRHTISMLEEDKLKLDKSRMNSYEECKSYKQDMEKLKTLNYELQRDKENLTDNCQDLDRNLERQTKEVERLTKSIENSESKSSRLKEEISSLKESLNRALLEKEVLEQENSVLTDSLNKTEVLKAEYEVKANKFKTEEARLRDKFHQLESMNHFLVKENSEFKTIIDQTESEKHRLSGEKKALEQEKYGIREELMRVENEKEELNQMKDGLHQSMQNVEKSYENLQDELSKLNYEKGQLETANVTLQRERNALSTELLQARRDAERLSAAVQKSMKDKEVLAKEKIDLISQLTTFEHELRENADAIYVLQEEKEQLDGSLYESQQNISQLEAKIEALESNLQQLERKRSIIFDEKQRLQHEFDVELQKLSKAKESLQIKLNESEKEWNKILRSEKEAREEDIERLIAEREELRNEFMKYKEEIKLQFQEEIDDLRHRLNRDKEELAQEIITIQRERDDCLLEAEMGKQHAINLLEQEKMSMYEKISQLTDLLRTSESDLERTRNECEIRAQQDQNTIQTFNGDLKKFRLQFEQIHIDHDREIKNLQCAYKELENQKYQDMRENEELKSLLKIAEETRDNLRQDLSESMGKIRETEDQRERLRRELMDEKRNYFEVKKELETIQLSNKELREKLKTSEMQKIDIQRQLQDLQNKLSILEEQKQVLQKEVKDTRYNLQDTEKARVDVRRELEELRSQVKKLDTERGKLVNEVSGLQVRVATDENKEVEARRENFGLKQKVIESEATKEELSKQLYQSLRKNEELEEILNSKEKTTKQYYDEVLKDYKSLEESKLNLENQLEITNHNFEELNSTLSTKENRISLLENQINILDQKRKGAENRLATIHSHLRKLIGFRQQICQTLRNRSPSPGRRSRSVSPEKGRVSSRNSVEFNFSEDRKRVGRFGDVNQYSE--------SVIPGS------DLDPEAVRIALRDITQHIVSIERERDDMEIKAKNSALKSDETSVQLDQALQRINQLQKNFGEVEEDKKGIDSRLSSAQTALMMQEETIHRNERERKSLIDKVGKLERQLAHVETEKKYLQERVDKMRTGESYHEEERRGLIKAVDDAENRCTQLELLRRGLEGELQRLKMGFNDKETENQVLRDRVDALTKQISDLESKAQSLQLTIDRLSMNLARTEEEENTQKDKVHQLNLSLSEHQGNLQSMQDKLVQLQSTLASSEHDRRVLQERLESVKHNLSEAKSQNHTLVDRMKATQQELMESESRKHEIEGEIKRLSQILSQRQEIEQELQSKIHRSAMEKNLLTERLNSLTASLAGLESERRDIEKVHIKTEKEKNSLKKTLDKLEKEKNRSEESINQSLLKRSEFDEFTRRLEDENEVLHRQVQKLQNQLAEAEQCHAQRIMELTSRIKNEMLSEIDRLKSALNSTEKTLQFRERSHRQKVKSLEEQINILKDQLSQELKKR-----ELNLNKSNRGLKNDICDIKSILEKNSNNISK-TSFDNLLYEPEIRRMKDILSESG 2164 The following BLAST results are available for this feature:
BLAST of Rootletin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Rootletin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of Rootletin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of Rootletin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of Rootletin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Rootletin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Rootletin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Rootletin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Rootletin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Rootletin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Rootletin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Rootletin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Rootletin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Rootletin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30030007 ID=SMED30030007|Name=Rootletin|organism=Schmidtea mediterranea sexual|type=transcript|length=6612bpback to top protein sequence of SMED30030007-orf-1 >SMED30030007-orf-1 ID=SMED30030007-orf-1|Name=SMED30030007-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2012bp MSEIDYSAIVRRRIGDIDGDSSEDSSSKGLLQVNLELRKRLEEEHNEYRRback to top Aliases
The feature 'Rootletin' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
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