Vesicle transport protein

Overview
NameVesicle transport protein
Smed IDSMED30030005
Length (bp)720
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vesicle transport protein (SMED30030005) t-SNE clustered cells

Violin plots show distribution of expression levels for Vesicle transport protein (SMED30030005) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vesicle transport protein (SMED30030005) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vesicle transport protein (SMED30030005) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30030005

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30030005 dd_Smed_v4_6215_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30030005 dd_Smed_v4_6215_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Vesicle transport protein vs. Ensembl Human
Match: SFT2D1 (SFT2 domain containing 1 [Source:HGNC Symbol;Acc:HGNC:21102])

HSP 1 Score: 47.3654 bits (111), Expect = 3.582e-6
Identity = 31/83 (37.35%), Postives = 47/83 (56.63%), Query Frame = 3 
Query:  309 RKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            + F + YT G+L  L S  FL GP   LK +F   R   T++ +   +  +  AL+ H   L+++F I+Q LS+ WY +SYIP
Sbjct:   62 KLFAVFYTLGNLAALASTCFLMGPVKQLKKMFEATRLLATIVMLLCFIFTLCAALWWHKKGLAVLFCILQFLSMTWYSLSYIP 144
[Close]
BLAST of Vesicle transport protein vs. Ensembl Celegans
Match: sftd-3 (Vesicle transport protein [Source:UniProtKB/TrEMBL;Acc:A0A2K5ATL4])

HSP 1 Score: 73.559 bits (179), Expect = 1.499e-15
Identity = 62/161 (38.51%), Postives = 94/161 (58.39%), Query Frame = 3 
Query:  159 SGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQS--LPI 635
            SGWF+ +  D     M+R  RI+ F + ++      S A +L+P+I+   RKF  L T GSL  L SF+FL GP ++L H+ S +R   TV Y+S L   +Y +L+L S I +LI  I Q  +L+WY++SY+PGG +G   +  +F +   ++  S  LPI
Sbjct:   75 SGWFSPSTQDESMFGMTRTQRIIAFFMCIIGAIFCFSTAAVLIPVILVSTRKFAGLNTLGSLLLLLSFAFLLGPKSYLTHMASPQRRLVTVSYLSALFATLYSSLWLKSTIFTLIAAIFQGFTLVWYVLSYVPGGERGLFFMTSLFTSFLRRSTTSTVLPI 235
[Close]
BLAST of Vesicle transport protein vs. Ensembl Celegans
Match: sftd-3 (Vesicle transport protein [Source:UniProtKB/TrEMBL;Acc:A0A2K5ATL4])

HSP 1 Score: 73.559 bits (179), Expect = 1.527e-15
Identity = 62/161 (38.51%), Postives = 94/161 (58.39%), Query Frame = 3 
Query:  159 SGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQS--LPI 635
            SGWF+ +  D     M+R  RI+ F + ++      S A +L+P+I+   RKF  L T GSL  L SF+FL GP ++L H+ S +R   TV Y+S L   +Y +L+L S I +LI  I Q  +L+WY++SY+PGG +G   +  +F +   ++  S  LPI
Sbjct:   79 SGWFSPSTQDESMFGMTRTQRIIAFFMCIIGAIFCFSTAAVLIPVILVSTRKFAGLNTLGSLLLLLSFAFLLGPKSYLTHMASPQRRLVTVSYLSALFATLYSSLWLKSTIFTLIAAIFQGFTLVWYVLSYVPGGERGLFFMTSLFTSFLRRSTTSTVLPI 239
[Close]
BLAST of Vesicle transport protein vs. Ensembl Celegans
Match: sftd-3 (Vesicle transport protein [Source:UniProtKB/TrEMBL;Acc:A0A2K5ATL4])

HSP 1 Score: 65.855 bits (159), Expect = 2.094e-13
Identity = 49/118 (41.53%), Postives = 72/118 (61.02%), Query Frame = 3 
Query:  288 PMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQS--LPI 635
            P+I+   RKF  L T GSL  L SF+FL GP ++L H+ S +R   TV Y+S L   +Y +L+L S I +LI  I Q  +L+WY++SY+PGG +G   +  +F +   ++  S  LPI
Sbjct:   35 PVILVSTRKFAGLNTLGSLLLLLSFAFLLGPKSYLTHMASPQRRLVTVSYLSALFATLYSSLWLKSTIFTLIAAIFQGFTLVWYVLSYVPGGERGLFFMTSLFTSFLRRSTTSTVLPI 152
[Close]
BLAST of Vesicle transport protein vs. Ensembl Celegans
Match: sftd-3 (Vesicle transport protein [Source:UniProtKB/TrEMBL;Acc:A0A2K5ATL4])

HSP 1 Score: 52.373 bits (124), Expect = 3.638e-9
Identity = 30/79 (37.97%), Postives = 47/79 (59.49%), Query Frame = 3 
Query:  405 SYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQS--LPI 635
            S +R   TV Y+S L   +Y +L+L S I +LI  I Q  +L+WY++SY+PGG +G   +  +F +   ++  S  LPI
Sbjct:    3 SPQRRLVTVSYLSALFATLYSSLWLKSTIFTLIAAIFQGFTLVWYVLSYVPGGERGLFFMTSLFTSFLRRSTTSTVLPI 81
[Close]
BLAST of Vesicle transport protein vs. Ensembl Fly
Match: CG33635 (gene:FBgn0053635 transcript:FBtr0091612)

HSP 1 Score: 65.0846 bits (157), Expect = 1.116e-12
Identity = 41/116 (35.34%), Postives = 69/116 (59.48%), Query Frame = 3 
Query:  288 PMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQSLPI 635
            P ++ KARKF +LYT GSL F+ SF FL G  + LK +FS  R   ++ Y S L++ +Y AL   S   +++F + Q+++L++ ++  +PGG  G K   ++F    + +   LP+
Sbjct:   84 PFLILKARKFALLYTLGSLFFILSFCFLSGFGSFLKQMFSKPRLLTSLSYSSCLILTLYCALVAKSTAFTVLFAVAQIIALLFMVLGTVPGGATGLKFFGQLFKNTVSASTSVLPV 199
[Close]
BLAST of Vesicle transport protein vs. Ensembl Fly
Match: CG5104 (gene:FBgn0037009 transcript:FBtr0078209)

HSP 1 Score: 46.9802 bits (110), Expect = 1.941e-6
Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFK 578
            F + YT G++  + S  FL GP+  +K +F+  R   T+I +  +++    A+      L+LIFII+Q L++ WY +SYIP      K
Sbjct:   67 FAVFYTLGNVISMASTCFLMGPFKQIKKMFAETRLIATIIVLVMMVLTFIAAIVWKKAGLTLIFIIIQSLAMTWYSLSYIPYARDAVK 154
[Close]
BLAST of Vesicle transport protein vs. Ensembl Zebrafish
Match: sft2d3 (SFT2 domain containing 3 [Source:ZFIN;Acc:ZDB-GENE-070907-1])

HSP 1 Score: 50.8322 bits (120), Expect = 2.958e-7
Identity = 45/159 (28.30%), Postives = 82/159 (51.57%), Query Frame = 3 
Query:  165 WFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAV--ATKTAQSLPI 635
            W   +E D     +SR  R++ F   +   +L   L+ L  P+++ KARKF +L++ GS+  L   + L GP      L +        +Y+ +L   +Y AL LHS +L+ +   +Q+ +++ YIV+ +PGG  G + +  +  +    T T +++PI
Sbjct:   71 WPWSSEPDPCLPGLSRSQRLVAFGTCIFFSALCFGLSALYAPLLLLKARKFALLWSLGSVFALLGAAILRGP----SKLIATPTPG-AAVYLCSLAGTLYAALSLHSTLLTALGACLQIAAIVGYIVALLPGGSAGMRFVGGMAASAIKRTVTGKTMPI 224
[Close]
BLAST of Vesicle transport protein vs. Ensembl Zebrafish
Match: sft2d2a (SFT2 domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-050227-13])

HSP 1 Score: 48.9062 bits (115), Expect = 7.372e-7
Identity = 31/81 (38.27%), Postives = 50/81 (61.73%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            FV+ YT G++C L S  FL GP   LK +    RA  TV+ ++ L++ +    +   + L+L+F+I+Q+L+  WY +SYIP
Sbjct:   67 FVVFYTLGNVCSLASTMFLMGPVKQLKRMCDKTRAFATVVMITCLVLTLCAVFWWKIFALTLLFVILQVLAFAWYGLSYIP 147
[Close]
BLAST of Vesicle transport protein vs. Ensembl Zebrafish
Match: sft2d2a (SFT2 domain containing 2a [Source:ZFIN;Acc:ZDB-GENE-050227-13])

HSP 1 Score: 48.1358 bits (113), Expect = 1.392e-6
Identity = 31/81 (38.27%), Postives = 50/81 (61.73%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            FV+ YT G++C L S  FL GP   LK +    RA  TV+ ++ L++ +    +   + L+L+F+I+Q+L+  WY +SYIP
Sbjct:   67 FVVFYTLGNVCSLASTMFLMGPVKQLKRMCDKTRAFATVVMITCLVLTLCAVFWWKIFALTLLFVILQVLAFAWYGLSYIP 147
[Close]
BLAST of Vesicle transport protein vs. Ensembl Zebrafish
Match: sft2d2b (SFT2 domain containing 2b [Source:ZFIN;Acc:ZDB-GENE-050522-336])

HSP 1 Score: 45.8246 bits (107), Expect = 7.647e-6
Identity = 29/81 (35.80%), Postives = 45/81 (55.56%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F + Y+ G++  L S  FL GP   LK +    RA  T I ++ L++    A +  +  L+L+F I+Q L+  WY +SYIP
Sbjct:   66 FAVFYSLGNIASLLSTMFLMGPLKQLKRMCDKTRALATGIMITCLVLTFCAAFWWKNRALALLFCILQFLAFTWYSISYIP 146
[Close]
BLAST of Vesicle transport protein vs. Ensembl Mouse
Match: Sft2d1 (SFT2 domain containing 1 [Source:MGI Symbol;Acc:MGI:1918689])

HSP 1 Score: 47.7506 bits (112), Expect = 1.855e-6
Identity = 33/81 (40.74%), Postives = 46/81 (56.79%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F + YT G+L  L S  FL GP   LK +F   R   T+I +  L+  +  AL+     L+L+F I+Q LS+ WY +SYIP
Sbjct:   64 FAVFYTLGNLAALASTCFLMGPVKQLKKMFETTRLLATIIMLLCLVFTLCAALWWRKKGLALLFCILQFLSMTWYSLSYIP 144
[Close]
BLAST of Vesicle transport protein vs. Ensembl Mouse
Match: Gm12166 (predicted gene 12166 [Source:MGI Symbol;Acc:MGI:3650635])

HSP 1 Score: 47.7506 bits (112), Expect = 1.855e-6
Identity = 33/81 (40.74%), Postives = 46/81 (56.79%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F + YT G+L  L S  FL GP   LK +F   R   T+I +  L+  +  AL+     L+L+F I+Q LS+ WY +SYIP
Sbjct:   64 FAVFYTLGNLAALASTCFLMGPVKQLKKMFETTRLLATIIMLLCLVFTLCAALWWRKKGLALLFCILQFLSMTWYSLSYIP 144
[Close]
BLAST of Vesicle transport protein vs. UniProt/SwissProt
Match: sp|P38166|SFT2_YEAST (Protein transport protein SFT2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SFT2 PE=1 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.726e-11
Identity = 37/99 (37.37%), Postives = 64/99 (64.65%), Query Frame = 3 
Query:  288 PMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKIL 584
            P++  K RKF +L+T GSL F+ +F  L GP  +LKHL + ER  F++ + +T  + IY A F  + +L++   +++L+++I+Y +SY P G  G ++L
Sbjct:  103 PVLAAKPRKFGLLWTMGSLLFVLAFGVLMGPLAYLKHLTARERLPFSMFFFATCFMTIYFAAFSKNTVLTITCALLELVAVIYYAISYFPFGATGLRML 201
[Close]
BLAST of Vesicle transport protein vs. UniProt/SwissProt
Match: sp|Q9P6K1|SFT2_SCHPO (Protein transport protein sft2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sft2 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.668e-7
Identity = 34/94 (36.17%), Postives = 57/94 (60.64%), Query Frame = 3 
Query:  288 PMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPK 569
            P++V K RKFV+L+T GSL  +  F+ + G   H + L + ER   T+ Y  TLL  I   + + S ILS++F ++ +LS + Y++++ P G +
Sbjct:   90 PVLVLKPRKFVLLWTMGSLLAVLGFAIVQGFVAHFRQLTTMERLPITLSYFVTLLATIIATIKIKSTILSIVFGVLHILSFVAYLIAFFPFGTR 183
[Close]
BLAST of Vesicle transport protein vs. TrEMBL
Match: A0A2T7PQ12 (Vesicle transport protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_02476 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 1.116e-32
Identity = 80/160 (50.00%), Postives = 111/160 (69.38%), Query Frame = 3 
Query:  159 SGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKT-AQSLPI 635
            +GWF  A+ D L  S+S++ RI+GFI+ LL+G+   SLA L +PM++ KARKF +LYT GS   + SF+FLWGPWNH+KHLFS ER  FT  Y   +   IY ++++ S IL++     Q+ +L+WYIVSYIPGG  G K    +FYA A+KT A++LP+
Sbjct:   51 NGWFTDAQKDPLLPSLSKKQRIIGFIMCLLMGTFCFSLAMLYIPMLILKARKFAMLYTLGSFFVIASFAFLWGPWNHMKHLFSMERLPFTATYFGAMFATIYFSIWVQSTILTVACAAAQVFALVWYIVSYIPGGQTGLKFFSNIFYAAASKTVAKALPV 210
[Close]
BLAST of Vesicle transport protein vs. TrEMBL
Match: A0A2G8JXC8 (Vesicle transport protein OS=Stichopus japonicus OX=307972 GN=BSL78_22746 PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.824e-31
Identity = 80/164 (48.78%), Postives = 110/164 (67.07%), Query Frame = 3 
Query:  147 QNIVSGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKT-AQSLPI 635
            Q+  +GWFN+A+ D  C ++S++ RILGF+LLL+ G     LA+L LPM+  K RKF +LYT GS+  + SFS LWGP NH+KHLFSY+R  FT +Y  T+   +Y      S +L+LI  I QLL+L+WY+VSYIPGG  G     K+F  VA KT + ++P+
Sbjct:   62 QDSTNGWFNQAQKDPFCPTLSKKQRILGFMLLLVSGLCCFILAWLFLPMLAIKPRKFALLYTMGSVFTIGSFSILWGPANHIKHLFSYQRLPFTAVYFGTIFATLYFVFVKRSTLLTLICSICQLLALVWYLVSYIPGGQTGLTFFTKLFATVAGKTISSTMPV 225
[Close]
BLAST of Vesicle transport protein vs. TrEMBL
Match: A0A2G8JBR0 (Vesicle transport protein OS=Stichopus japonicus OX=307972 GN=BSL78_30009 PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.074e-31
Identity = 80/164 (48.78%), Postives = 110/164 (67.07%), Query Frame = 3 
Query:  147 QNIVSGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKT-AQSLPI 635
            Q+  +GWFN+A+ D  C ++S++ RILGF+LLL+ G     LA+L LPM+  K RKF +LYT GS+  + SFS LWGP NH+KHLFSY+R  FT +Y  T+   +Y      S +L+LI  I QLL+L+WY+VSYIPGG  G     K+F  VA KT + ++P+
Sbjct:   62 QDSTNGWFNQAQKDPFCPTLSKKQRILGFMLLLVSGLGCFILAWLFLPMLAIKPRKFALLYTMGSVFTIGSFSILWGPANHIKHLFSYQRLPFTAVYFGTIFATLYFVFVKRSTLLTLICSICQLLALVWYLVSYIPGGQTGLTFFTKLFATVAGKTISSTMPV 225
[Close]
BLAST of Vesicle transport protein vs. TrEMBL
Match: V4A9J9 (Vesicle transport protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_192494 PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 1.150e-30
Identity = 80/161 (49.69%), Postives = 111/161 (68.94%), Query Frame = 3 
Query:  156 VSGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQS-LPI 635
             + WF +A+ND L  S+ ++ RI+GFI+ LL+G+   SLA L LPM+ FKARKF  LYT GS+  + SFSFLWGP +H+KHLFS  R  FT+ Y  T+   +Y +L++H  + ++IF   Q+++L+WY VSYIPGG  G K   KVFYA A+KT  + LP+
Sbjct:   22 ANAWFQQAQNDPLLPSLGKQQRIIGFIVCLLMGTFCFSLAGLYLPMLAFKARKFAALYTLGSIFVISSFSFLWGPVHHVKHLFSGPRLPFTIAYFGTMFATLYFSLWVHKTLFTIIFAFAQIIALVWYFVSYIPGGQTGLKFFTKVFYAAASKTVNATLPV 182
[Close]
BLAST of Vesicle transport protein vs. TrEMBL
Match: A0A2C9KWX8 (Vesicle transport protein OS=Biomphalaria glabrata OX=6526 GN=106050793 PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.156e-30
Identity = 78/163 (47.85%), Postives = 112/163 (68.71%), Query Frame = 3 
Query:  150 NIVSGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKT-AQSLPI 635
               + WF  A++D  C S+S++ RI+GFI+ LL+G+  +SLA L +P +V KARKF +LYT GSL  L SF+ LWGP NH KHLFS  R  FT  Y   +L  +Y +L++ S +L+++F + Q+++LIWY+VSYIPGG  G K    +FYA A+KT  ++LP+
Sbjct:   46 EAANSWFIDAQSDPFCPSLSKKQRIIGFIICLLMGTFCLSLASLYIPFLVLKARKFAMLYTLGSLFVLSSFALLWGPMNHFKHLFSVGRLPFTTTYFGAMLATLYFSLWVQSTVLTIVFAVAQIVALIWYLVSYIPGGQTGLKFFTSIFYAAASKTVGKTLPM 208
[Close]
BLAST of Vesicle transport protein vs. Ensembl Cavefish
Match: sft2d2a (vesicle transport protein SFT2B-like [Source:NCBI gene;Acc:103032030])

HSP 1 Score: 46.2098 bits (108), Expect = 5.327e-6
Identity = 31/81 (38.27%), Postives = 52/81 (64.20%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F++ YTFG++C LFS  FL GP   +K +    RA  TV+ ++ L++ +    +   + L+L+F+I+Q+L+  WY +SYIP
Sbjct:   67 FIVFYTFGNICSLFSTMFLMGPVKQIKKMCDKTRAFATVVMITCLVLTLCAVFWWKIFALTLLFVILQVLAFAWYGLSYIP 147
[Close]
BLAST of Vesicle transport protein vs. Ensembl Cavefish
Match: sft2d2b (vesicle transport protein SFT2B-like [Source:NCBI gene;Acc:103042672])

HSP 1 Score: 45.4394 bits (106), Expect = 8.861e-6
Identity = 30/81 (37.04%), Postives = 47/81 (58.02%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F + YTFG++  L S  FL GP   +K +    RA  T I ++ L++ +  A +  +  L+L+F I+Q L+  WY +SYIP
Sbjct:   67 FAVFYTFGNIASLLSTMFLMGPLKQVKRMCDKTRALATAIMITCLVLTLCAAFWWKNKGLALLFCILQFLAFTWYGLSYIP 147
[Close]
BLAST of Vesicle transport protein vs. Ensembl Sea Lamprey
Match: sft2d1 (SFT2 domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1350])

HSP 1 Score: 46.2098 bits (108), Expect = 1.009e-6
Identity = 30/81 (37.04%), Postives = 48/81 (59.26%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F + Y+ G++C L S  FL GP   +K +F+  RA  T + +  L+  +  A +  +  L+L+F ++Q LSL WY +SYIP
Sbjct:   65 FAVFYSLGNICALCSTCFLMGPLKQMKRMFNPTRAIATSVMLCCLVCTLCSAFWWKNKPLALLFCVLQFLSLTWYSLSYIP 145
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BLAST of Vesicle transport protein vs. Ensembl Yeast
Match: SFT2 (Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 [Source:SGD;Acc:S000000198])

HSP 1 Score: 64.3142 bits (155), Expect = 3.926e-13
Identity = 37/99 (37.37%), Postives = 64/99 (64.65%), Query Frame = 3 
Query:  288 PMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKIL 584
            P++  K RKF +L+T GSL F+ +F  L GP  +LKHL + ER  F++ + +T  + IY A F  + +L++   +++L+++I+Y +SY P G  G ++L
Sbjct:  103 PVLAAKPRKFGLLWTMGSLLFVLAFGVLMGPLAYLKHLTARERLPFSMFFFATCFMTIYFAAFSKNTVLTITCALLELVAVIYYAISYFPFGATGLRML 201
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BLAST of Vesicle transport protein vs. Ensembl Nematostella
Match: EDO46566 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPU0])

HSP 1 Score: 116.316 bits (290), Expect = 1.197e-32
Identity = 77/160 (48.12%), Postives = 103/160 (64.38%), Query Frame = 3 
Query:  159 SGWFNKAENDFLCTSMSRRTRXXXXXXXXXXXXXXXXXXXXXXPMIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYAVATKTAQS-LPI 635
              W +K E D    S+SR+ RILGF + LL+G     +A  + P IV KARKFV+LYT GSL  + SFS LWGP NH+KHL S  R  FT  Y  +++  +Y+AL + S IL+ +F +VQ+++LIWY VSYIPGG  G K   ++F +  TKT  S LP+
Sbjct:   12 GAWLSKGEEDPYLPSLSRKQRILGFFMCLLMGVFCFVMAGFIAPFIVLKARKFVMLYTMGSLFTIGSFSLLWGPVNHVKHLCSLGRLPFTAAYFGSMIATLYMALIVRSTILTALFAVVQIIALIWYFVSYIPGGTTGMKFFTRLFSSAVTKTVSSTLPV 171
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BLAST of Vesicle transport protein vs. Ensembl Nematostella
Match: EDO38593 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCF2])

HSP 1 Score: 55.0694 bits (131), Expect = 1.035e-9
Identity = 33/96 (34.38%), Postives = 57/96 (59.38%), Query Frame = 3 
Query:  291 MIVFKARKFVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFK 578
            +++   + F + YT G++  L S  FL GP   LK++F  +R   T++ ++ L++ +  AL+ HS  L+++F I+Q LS+ WY +SYIP      K
Sbjct:   59 LLIRDIKLFAVFYTLGNITALLSTCFLMGPCKQLKNMFKEKRLIATILMLTCLVLTLCAALWWHSKGLAIVFCILQYLSMTWYCLSYIPFARDAVK 154
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BLAST of Vesicle transport protein vs. Ensembl Medaka
Match: sft2d2a (vesicle transport protein SFT2B-like [Source:NCBI gene;Acc:101171820])

HSP 1 Score: 49.6766 bits (117), Expect = 2.753e-7
Identity = 34/81 (41.98%), Postives = 51/81 (62.96%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F+I YTFG++C L S  FL GP   LK +    RA  T I ++ L++ +  A +  ++ L+L+F I+Q+LS  WY +SYIP
Sbjct:   66 FIIFYTFGNICALGSTMFLMGPVKQLKRMCDKTRALATTIMITCLVLTLCAAFWWKNFGLALLFCILQVLSFAWYSLSYIP 146
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BLAST of Vesicle transport protein vs. Ensembl Medaka
Match: ENSORLT00000046729.1 (vesicle transport protein SFT2B [Source:NCBI gene;Acc:101155498])

HSP 1 Score: 47.7506 bits (112), Expect = 1.152e-6
Identity = 31/81 (38.27%), Postives = 49/81 (60.49%), Query Frame = 3 
Query:  315 FVILYTXXXXXXXXXXXXXWGPWNHLKHLFSYERASFTVIYVSTLLINIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIP 557
            F +LY+ G++  L S  FL GP   +K + + ERA  TVI +  L++ +  A +  +  L+L+F I+Q L+  WY +SYIP
Sbjct:   64 FAVLYSVGNISALASTMFLMGPLRQVKKMCAKERALATVIMLVCLVLTLCAAFWWKNKGLALLFCILQFLAFTWYGLSYIP 144
The following BLAST results are available for this feature:
BLAST of Vesicle transport protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SFT2D13.582e-637.35SFT2 domain containing 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of Vesicle transport protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
sftd-31.499e-1538.51Vesicle transport protein [Source:UniProtKB/TrEMB... [more]
sftd-31.527e-1538.51Vesicle transport protein [Source:UniProtKB/TrEMB... [more]
sftd-32.094e-1341.53Vesicle transport protein [Source:UniProtKB/TrEMB... [more]
sftd-33.638e-937.97Vesicle transport protein [Source:UniProtKB/TrEMB... [more]
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BLAST of Vesicle transport protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG336351.116e-1235.34gene:FBgn0053635 transcript:FBtr0091612[more]
CG51041.941e-634.09gene:FBgn0037009 transcript:FBtr0078209[more]
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BLAST of Vesicle transport protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
sft2d32.958e-728.30SFT2 domain containing 3 [Source:ZFIN;Acc:ZDB-GENE... [more]
sft2d2a7.372e-738.27SFT2 domain containing 2a [Source:ZFIN;Acc:ZDB-GEN... [more]
sft2d2a1.392e-638.27SFT2 domain containing 2a [Source:ZFIN;Acc:ZDB-GEN... [more]
sft2d2b7.647e-635.80SFT2 domain containing 2b [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of Vesicle transport protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vesicle transport protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Sft2d11.855e-640.74SFT2 domain containing 1 [Source:MGI Symbol;Acc:MG... [more]
Gm121661.855e-640.74predicted gene 12166 [Source:MGI Symbol;Acc:MGI:36... [more]
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BLAST of Vesicle transport protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|P38166|SFT2_YEAST2.726e-1137.37Protein transport protein SFT2 OS=Saccharomyces ce... [more]
sp|Q9P6K1|SFT2_SCHPO1.668e-736.17Protein transport protein sft2 OS=Schizosaccharomy... [more]
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BLAST of Vesicle transport protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7PQ121.116e-3250.00Vesicle transport protein OS=Pomacea canaliculata ... [more]
A0A2G8JXC81.824e-3148.78Vesicle transport protein OS=Stichopus japonicus O... [more]
A0A2G8JBR02.074e-3148.78Vesicle transport protein OS=Stichopus japonicus O... [more]
V4A9J91.150e-3049.69Vesicle transport protein OS=Lottia gigantea OX=22... [more]
A0A2C9KWX81.156e-3047.85Vesicle transport protein OS=Biomphalaria glabrata... [more]
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BLAST of Vesicle transport protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
sft2d2a5.327e-638.27vesicle transport protein SFT2B-like [Source:NCBI ... [more]
sft2d2b8.861e-637.04vesicle transport protein SFT2B-like [Source:NCBI ... [more]
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BLAST of Vesicle transport protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sft2d11.009e-637.04SFT2 domain containing 1 [Source:ZFIN;Acc:ZDB-GENE... [more]
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BLAST of Vesicle transport protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
SFT23.926e-1337.37Tetra-spanning membrane protein found mostly in th... [more]
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BLAST of Vesicle transport protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO465661.197e-3248.13Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO385931.035e-934.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vesicle transport protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
sft2d2a2.753e-741.98vesicle transport protein SFT2B-like [Source:NCBI ... [more]
ENSORLT00000046729.11.152e-638.27vesicle transport protein SFT2B [Source:NCBI gene;... [more]
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BLAST of Vesicle transport protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30030005 ID=SMED30030005|Name=Vesicle transport protein|organism=Schmidtea mediterranea sexual|type=transcript|length=720bp
CATTACTATATGTACAAATGAATTTATTTAAAAGTCAACAGACCCTTCCT
TTGTACAATTCTTCTAATGGATCGAATAATTATACATATTCGCCTTTATC
GTTTTTTAGTTCAAATAGCCTTTCAAAAAGTGATCCGAATGAATCCCAAA
ATATAGTTAGTGGATGGTTCAACAAAGCAGAAAATGATTTTCTATGCACT
TCGATGAGCAGACGAACTCGAATTCTCGGATTTATATTACTGCTTTTACT
TGGAAGTTTATTTGTTAGCTTAGCTTTCCTTTTATTGCCAATGATAGTAT
TTAAAGCCAGAAAATTTGTTATTTTGTACACTTTTGGTTCACTATGTTTT
CTATTTAGTTTTTCATTTTTATGGGGACCTTGGAATCACCTGAAGCACTT
ATTTAGCTACGAACGAGCATCTTTTACTGTGATATATGTAAGCACATTAC
TTATCAATATCTATGTTGCATTGTTTCTACATAGTTGGATATTGTCATTG
ATTTTTATTATTGTACAACTTTTATCGCTGATTTGGTATATTGTCAGTTA
TATTCCAGGTGGACCCAAAGGTTTTAAAATATTGTTGAAAGTATTTTACG
CTGTTGCAACTAAGACAGCACAGTCTTTACCAATATGAATATATTTTATT
ATTCGTTATGCTTTGTTAATTAAAGAATTTAATAAATAAATTTATATATT
ATCCCGATTAATGTAACATG
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protein sequence of SMED30030005-orf-1

>SMED30030005-orf-1 ID=SMED30030005-orf-1|Name=SMED30030005-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=212bp
LLYVQMNLFKSQQTLPLYNSSNGSNNYTYSPLSFFSSNSLSKSDPNESQN
IVSGWFNKAENDFLCTSMSRRTRILGFILLLLLGSLFVSLAFLLLPMIVF
KARKFVILYTFGSLCFLFSFSFLWGPWNHLKHLFSYERASFTVIYVSTLL
INIYVALFLHSWILSLIFIIVQLLSLIWYIVSYIPGGPKGFKILLKVFYA
VATKTAQSLPI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR011691Vesicle_transpt_SFT2
IPR023795Serpin_CS
IPR007305Vesicle_transpt_Got1/SFT2
Vocabulary: biological process
TermDefinition
GO:0016192vesicle-mediated transport
GO:0006810transport
GO:0015031protein transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007305Vesicle transport protein, Got1/SFT2-likePFAMPF04178Got1coord: 84..194
e-value: 3.1E-32
score: 111.3
NoneNo IPR availablePANTHERPTHR23137:SF6VESICLE TRANSPORT PROTEIN SFT2Ccoord: 45..202
NoneNo IPR availableTMHMMTMhelixcoord: 162..184
NoneNo IPR availableTMHMMTMhelixcoord: 139..158
NoneNo IPR availableTMHMMTMhelixcoord: 78..100
NoneNo IPR availableTMHMMTMhelixcoord: 105..127
IPR011691Vesicle transport protein SFT2PANTHERPTHR23137UNCHARACTERIZEDcoord: 45..202