G-patch domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30029912 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of G-patch domain-containing protein vs. Ensembl Human
Match: GPKOW (G-patch domain and KOW motifs [Source:HGNC Symbol;Acc:HGNC:30677]) HSP 1 Score: 53.9138 bits (128), Expect = 2.144e-7 Identity = 23/55 (41.82%), Postives = 33/55 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSML 865 +W+ L VR +D Y+ G+Y N ++I+DVL PD C C T VL +R+ ML Sbjct: 356 HWLHRDLRVRFVDNMYKGGQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDML 410
BLAST of G-patch domain-containing protein vs. Ensembl Zebrafish
Match: gpkow (G patch domain and KOW motifs [Source:NCBI gene;Acc:368735]) HSP 1 Score: 53.1434 bits (126), Expect = 2.656e-7 Identity = 23/60 (38.33%), Postives = 35/60 (58.33%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 +W+ L VR ID +++ G+Y N + ++DVL P C C T +L D+RQ ML + P Sbjct: 388 SWLQRDLRVRFIDKAFKGGKYYNSKMRVEDVLTPHTCVCRTEEGRMLDDIRQKMLETIVP 447
BLAST of G-patch domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000032061.1 (G-patch domain and KOW motifs [Source:NCBI gene;Acc:496860]) HSP 1 Score: 59.6918 bits (143), Expect = 3.392e-9 Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 W+ L VR ID +Y+ G+Y N + I+DVL P CSC T + +L D+RQ ML + P Sbjct: 380 GWLHRDLRVRFIDKNYKGGKYYNSKMFIEDVLSPTRCSCRTESGRILDDMRQDMLETIIP 439
BLAST of G-patch domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000017333.1 (G-patch domain and KOW motifs [Source:NCBI gene;Acc:496860]) HSP 1 Score: 59.3066 bits (142), Expect = 3.557e-9 Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 W+ L VR ID +Y+ G+Y N + I+DVL P CSC T + +L D+RQ ML + P Sbjct: 341 GWLHRDLRVRFIDKNYKGGKYYNSKMFIEDVLSPTRCSCRTESGRILDDMRQDMLETIIP 400
BLAST of G-patch domain-containing protein vs. Ensembl Mouse
Match: Gpkow (G patch domain and KOW motifs [Source:MGI Symbol;Acc:MGI:1859610]) HSP 1 Score: 60.8474 bits (146), Expect = 1.470e-10 Identity = 54/187 (28.88%), Postives = 79/187 (42.25%), Query Frame = 2 Query: 446 PEQILINDLVVINNGKFSGYIGKVIYLSGDSDLVRVYVE------------------ELQKEVNVRSAAVEAISNDEINIGGNSKVXXXXXXXXXXXXDRVVD----------NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRKQH-AKVLGGPH 919 P+ ++ VV+ +G + G GKV L D D VR V E Q +++++A N E + K + +D V +W+ L VR ID +++GRY N + I+DVL PD C C T VL DVR+ ML + P + H V+ GPH Sbjct: 5 PQGLMHGRAVVVLSGPYRGLYGKVEGL--DPDNVRAMVRLAVGNRIVTVSHVSQTSTEQQNRATGTASSLKAAQNQE-DSKRRQKGSEKKRKHSPDRQDGPVPKTEKAAPRNKHWLHRDLRVRFIDKLHKSGRYYNTKMTIEDVLSPDTCVCRTDEGRVLEDVREDMLETLIPKGEGHRVMVVLGPH 188
BLAST of G-patch domain-containing protein vs. Ensembl Mouse
Match: Gpkow (G patch domain and KOW motifs [Source:MGI Symbol;Acc:MGI:1859610]) HSP 1 Score: 61.6178 bits (148), Expect = 6.392e-10 Identity = 32/77 (41.56%), Postives = 44/77 (57.14%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRKQH-AKVLGGPHLSR 928 +W+ L VR ID +++GRY N + I+DVL PD C C T VL DVR+ ML + P + H V+ GPH + Sbjct: 368 HWLHRDLRVRFIDKLHKSGRYYNTKMTIEDVLSPDTCVCRTDEGRVLEDVREDMLETLIPKGEGHRVMVVLGPHAGK 444
BLAST of G-patch domain-containing protein vs. UniProt/SwissProt
Match: sp|Q56A08|GPKOW_MOUSE (G-patch domain and KOW motifs-containing protein OS=Mus musculus OX=10090 GN=Gpkow PE=1 SV=2) HSP 1 Score: 61.6178 bits (148), Expect = 4.471e-9 Identity = 32/77 (41.56%), Postives = 44/77 (57.14%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRKQH-AKVLGGPHLSR 928 +W+ L VR ID +++GRY N + I+DVL PD C C T VL DVR+ ML + P + H V+ GPH + Sbjct: 368 HWLHRDLRVRFIDKLHKSGRYYNTKMTIEDVLSPDTCVCRTDEGRVLEDVREDMLETLIPKGEGHRVMVVLGPHAGK 444
BLAST of G-patch domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6NU07|GPKOW_XENLA (G-patch domain and KOW motifs-containing protein OS=Xenopus laevis OX=8355 GN=gpkow PE=2 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 1.673e-7 Identity = 24/60 (40.00%), Postives = 36/60 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 W+ + VR ID +Y+ G+Y N ++++DVL P C C T N +L D+RQ ML + P Sbjct: 369 GWLRRDIRVRFIDKNYKGGKYYNSKMLVEDVLSPTRCVCRTENGCILEDIRQDMLETIIP 428
BLAST of G-patch domain-containing protein vs. UniProt/SwissProt
Match: sp|Q92917|GPKOW_HUMAN (G-patch domain and KOW motifs-containing protein OS=Homo sapiens OX=9606 GN=GPKOW PE=1 SV=2) HSP 1 Score: 53.9138 bits (128), Expect = 1.030e-6 Identity = 23/55 (41.82%), Postives = 33/55 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSML 865 +W+ L VR +D Y+ G+Y N ++I+DVL PD C C T VL +R+ ML Sbjct: 356 HWLHRDLRVRFVDNMYKGGQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDML 410
BLAST of G-patch domain-containing protein vs. UniProt/SwissProt
Match: sp|Q90X38|GPKOW_DANRE (G-patch domain and KOW motifs-containing protein OS=Danio rerio OX=7955 GN=gpkow PE=2 SV=2) HSP 1 Score: 53.1434 bits (126), Expect = 2.041e-6 Identity = 23/60 (38.33%), Postives = 35/60 (58.33%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 +W+ L VR ID +++ G+Y N + ++DVL P C C T +L D+RQ ML + P Sbjct: 388 SWLQRDLRVRFIDKAFKGGKYYNSKMRVEDVLTPHTCVCRTEEGRMLDDIRQKMLETIVP 447
BLAST of G-patch domain-containing protein vs. TrEMBL
Match: F6XU64 (G-patch domain and KOW motifs-containing protein (Fragment) OS=Mus musculus OX=10090 GN=Gpkow PE=1 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 3.047e-7 Identity = 54/187 (28.88%), Postives = 79/187 (42.25%), Query Frame = 2 Query: 446 PEQILINDLVVINNGKFSGYIGKVIYLSGDSDLVRVYVE------------------ELQKEVNVRSAAVEAISNDEINIGGNSKVXXXXXXXXXXXXDRVVD----------NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRKQH-AKVLGGPH 919 P+ ++ VV+ +G + G GKV L D D VR V E Q +++++A N E + K + +D V +W+ L VR ID +++GRY N + I+DVL PD C C T VL DVR+ ML + P + H V+ GPH Sbjct: 5 PQGLMHGRAVVVLSGPYRGLYGKVEGL--DPDNVRAMVRLAVGNRIVTVSHVSQTSTEQQNRATGTASSLKAAQNQE-DSKRRQKGSEKKRKHSPDRQDGPVPKTEKAAPRNKHWLHRDLRVRFIDKLHKSGRYYNTKMTIEDVLSPDTCVCRTDEGRVLEDVREDMLETLIPKGEGHRVMVVLGPH 188
BLAST of G-patch domain-containing protein vs. TrEMBL
Match: A0A1A6HSE8 (G-patch domain-containing protein OS=Neotoma lepida OX=56216 GN=A6R68_20666 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 1.965e-6 Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRK-QHAKVLGGPHLSR 928 +W+ L VR ID ++ GRY N + I+DVL PD C C T VL VR+ ML + P + H V+ GPH + Sbjct: 368 HWLHRDLRVRFIDKLHKGGRYYNTKMTIEDVLSPDTCVCRTDEGRVLEGVREGMLETLIPKEEGHHVMVVLGPHAGK 444
BLAST of G-patch domain-containing protein vs. TrEMBL
Match: A0A5G3JBT6 (G-patch domain and KOW motifs OS=Xenopus tropicalis OX=8364 GN=gpkow PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 5.326e-6 Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 W+ L VR ID +Y+ G+Y N + I+DVL P CSC T + +L D+RQ ML + P Sbjct: 380 GWLHRDLRVRFIDKNYKGGKYYNSKMFIEDVLSPTRCSCRTESGRILDDMRQDMLETIIP 439
BLAST of G-patch domain-containing protein vs. TrEMBL
Match: A0A5G3JAH1 (G-patch domain and KOW motifs OS=Xenopus tropicalis OX=8364 GN=gpkow PE=4 SV=1) HSP 1 Score: 59.3066 bits (142), Expect = 5.585e-6 Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 W+ L VR ID +Y+ G+Y N + I+DVL P CSC T + +L D+RQ ML + P Sbjct: 341 GWLHRDLRVRFIDKNYKGGKYYNSKMFIEDVLSPTRCSCRTESGRILDDMRQDMLETIIP 400
BLAST of G-patch domain-containing protein vs. TrEMBL
Match: C3Z963 (G-patch domain-containing protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_174745 PE=4 SV=1) HSP 1 Score: 59.3066 bits (142), Expect = 5.827e-6 Identity = 28/59 (47.46%), Postives = 37/59 (62.71%), Query Frame = 2 Query: 704 WMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 WM L VR+ID Y+NG+Y N V IDDV + C+C+T + V+ V+QSML V P Sbjct: 361 WMRPQLRVRVIDKHYKNGKYYNAKVAIDDVTDAETCTCVTEDGRVIDGVKQSMLETVIP 419
BLAST of G-patch domain-containing protein vs. Ensembl Sea Lamprey
Match: gpkow (G patch domain and KOW motifs [Source:NCBI gene;Acc:368735]) HSP 1 Score: 51.6026 bits (122), Expect = 1.775e-7 Identity = 22/59 (37.29%), Postives = 34/59 (57.63%), Query Frame = 2 Query: 704 WMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 W+ L VR +D Y+ G+Y N+ + +DDV+ P+ C C T +L + QSML + P Sbjct: 410 WLRRDLRVRFVDKFYKGGKYYNKKMTVDDVVTPEICVCRTEEGRILEGIEQSMLETIIP 468
BLAST of G-patch domain-containing protein vs. Ensembl Nematostella
Match: EDO48178 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK31]) HSP 1 Score: 53.9138 bits (128), Expect = 5.572e-8 Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 2 Query: 704 WMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRKQHA--KVLGGP 916 W+ L VR+I Y+ GRY N V + DV+ +EC C T + +L V+QSML V P +KQ A +++ GP Sbjct: 366 WLHPELRVRIISKDYKKGRYYNTKVKVVDVISRNECDCETEDGKLLEGVKQSMLETVVP-KKQDAYIRIVRGP 437
BLAST of G-patch domain-containing protein vs. Ensembl Medaka
Match: gpkow (G-patch domain and KOW motifs [Source:NCBI gene;Acc:101167619]) HSP 1 Score: 50.8322 bits (120), Expect = 1.253e-6 Identity = 23/60 (38.33%), Postives = 35/60 (58.33%), Query Frame = 2 Query: 701 NWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQP 880 +W+ L VR ID +++ GR+ N + ++DVL P C C T +L DV+Q ML + P Sbjct: 394 SWLQRDLKVRFIDKAFKGGRFYNSKMQVEDVLTPFTCVCRTEEGRLLDDVKQDMLETIIP 453
BLAST of G-patch domain-containing protein vs. Planmine SMEST
Match: SMESG000080913.1 (SMESG000080913.1) HSP 1 Score: 558.525 bits (1438), Expect = 0.000e+0 Identity = 327/331 (98.79%), Postives = 329/331 (99.40%), Query Frame = 2 Query: 62 MISFSFNKKISDTKNKIIKQKEKVEFISSFSNDNAVVLPAKYPSNASCSYKRKRKCIMNKTDVKNFVTNDIENTSASESKDFLKSLLNTKMKLTENDNYDRTLKLFSNNSLCXXXXXXXXXXXXXXXXPEQILINDLVVINNGKFSGYIGKVIYLSGDSDLVRVYVEELQKEVNVRSAAVEAISNDEINIGGNSKVXXXXXXXXXXXXDRVVDNWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQPIRKQHAKVLGGPHLSRXXXXXXXXXXXXXXXXXNSEEKFAIDIRLLCRMSSYDHVGPA 1054 MISFSFNKKISDTKNKIIKQKEKVE+IS FSNDNAVVLPAKYPSNASCSYKRKRKCIMNKTDVKNFVTNDIENTSASESKDFLKSLLNTKMKLTENDNYDRTLKLFSNNSLCKQKQNRKRRKGSSKKKPEQI+INDLVVINNGKFSGYIGKVIYLSGDSDLVRVYVEELQKEVNVRSAAVEAISNDEINIGGNSKVISPMESIEIEMEDRVVDNWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQ IRKQHAKVLGGPHLSRVVLVLAIDDLVATVVDVNSEEKFAIDIRLLCRMSSYDHVGPA Sbjct: 1 MISFSFNKKISDTKNKIIKQKEKVEYISRFSNDNAVVLPAKYPSNASCSYKRKRKCIMNKTDVKNFVTNDIENTSASESKDFLKSLLNTKMKLTENDNYDRTLKLFSNNSLCKQKQNRKRRKGSSKKKPEQIVINDLVVINNGKFSGYIGKVIYLSGDSDLVRVYVEELQKEVNVRSAAVEAISNDEINIGGNSKVISPMESIEIEMEDRVVDNWMFVGLCVRLIDGSYQNGRYDNETVVIDDVLGPDECSCITSNKNVLYDVRQSMLIRVQLIRKQHAKVLGGPHLSRVVLVLAIDDLVATVVDVNSEEKFAIDIRLLCRMSSYDHVGPA 331 The following BLAST results are available for this feature:
BLAST of G-patch domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of G-patch domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of G-patch domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of G-patch domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of G-patch domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 2
BLAST of G-patch domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of G-patch domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 4
BLAST of G-patch domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of G-patch domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of G-patch domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of G-patch domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of G-patch domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of G-patch domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of G-patch domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30029912 ID=SMED30029912|Name=G-patch domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1081bpback to top protein sequence of SMED30029912-orf-1 >SMED30029912-orf-1 ID=SMED30029912-orf-1|Name=SMED30029912-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=332bp MISFSFNKKISDTKNKIIKQKEKVEFISSFSNDNAVVLPAKYPSNASCSYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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