SMED30029875

Overview
NameSMED30029875
Smed IDSMED30029875
Length (bp)1009
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30029875 (SMED30029875) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30029875 (SMED30029875) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30029875

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30029875SMESG000077977.1 Contig36825newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30029875SMESG000077977.1 Contig36825uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
musculature systemSMED30029875SMESG000077977.1 dd_9565dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30029875 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000969:14570..15622 +4934
v31.000969:32190..33242 +4925
v31.000969:52217..53276 +4907
v31.000969:43902..44954 +4907
v31.000969:20796..21848 +4889
Homology
[Close]
BLAST of SMED30029875 vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 341.273 bits (874), Expect = 7.012e-135
Identity = 184/202 (91.09%), Postives = 186/202 (92.08%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:  497 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 683

HSP 2 Score: 111.309 bits (277), Expect = 7.012e-135
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  680 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 750

HSP 3 Score: 69.707 bits (169), Expect = 7.012e-135
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  468 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 498

HSP 4 Score: 374.4 bits (960), Expect = 1.826e-130
Identity = 200/217 (92.17%), Postives = 202/217 (93.09%), Query Frame = -2 
Query:  256 MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 906
            MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD+SIKFRVRNNYID+
Sbjct:    1 MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDKSIKFRVRNNYIDL 202

HSP 5 Score: 111.309 bits (277), Expect = 1.826e-130
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  199 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 269
[Close]
BLAST of SMED30029875 vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 342.043 bits (876), Expect = 1.241e-134
Identity = 184/202 (91.09%), Postives = 187/202 (92.57%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD+SIKFRVRNNYID+
Sbjct:  497 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDKSIKFRVRNNYIDL 683

HSP 2 Score: 110.538 bits (275), Expect = 1.241e-134
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  680 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 750

HSP 3 Score: 69.3218 bits (168), Expect = 1.241e-134
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  468 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 498

HSP 4 Score: 341.273 bits (874), Expect = 2.024e-134
Identity = 184/202 (91.09%), Postives = 186/202 (92.08%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:  978 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 1164

HSP 5 Score: 110.538 bits (275), Expect = 2.024e-134
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct: 1161 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 1231

HSP 6 Score: 69.3218 bits (168), Expect = 2.024e-134
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  949 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 979

HSP 7 Score: 372.859 bits (956), Expect = 8.359e-130
Identity = 200/217 (92.17%), Postives = 201/217 (92.63%), Query Frame = -2 
Query:  256 MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 906
            MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:    1 MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 202

HSP 8 Score: 110.538 bits (275), Expect = 8.359e-130
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  199 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 269
[Close]
BLAST of SMED30029875 vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 342.043 bits (876), Expect = 1.896e-134
Identity = 184/202 (91.09%), Postives = 187/202 (92.57%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD+SIKFRVRNNYID+
Sbjct:  492 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDKSIKFRVRNNYIDL 678

HSP 2 Score: 109.768 bits (273), Expect = 1.896e-134
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  675 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 745

HSP 3 Score: 68.9366 bits (167), Expect = 1.896e-134
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  463 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 493

HSP 4 Score: 341.273 bits (874), Expect = 3.017e-134
Identity = 184/202 (91.09%), Postives = 186/202 (92.08%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:  973 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 1159

HSP 5 Score: 109.768 bits (273), Expect = 3.017e-134
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct: 1156 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 1226

HSP 6 Score: 68.9366 bits (167), Expect = 3.017e-134
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  944 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 974

HSP 7 Score: 364.385 bits (934), Expect = 4.808e-127
Identity = 195/212 (91.98%), Postives = 196/212 (92.45%), Query Frame = -2 
Query:  256 MALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 891
            MALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:    1 MALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 197

HSP 8 Score: 109.768 bits (273), Expect = 4.808e-127
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  194 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 264
[Close]
BLAST of SMED30029875 vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 342.043 bits (876), Expect = 1.928e-134
Identity = 184/202 (91.09%), Postives = 187/202 (92.57%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD+SIKFRVRNNYID+
Sbjct:  497 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDKSIKFRVRNNYIDL 683

HSP 2 Score: 109.768 bits (273), Expect = 1.928e-134
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  680 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 750

HSP 3 Score: 68.9366 bits (167), Expect = 1.928e-134
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  468 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 498

HSP 4 Score: 341.273 bits (874), Expect = 3.093e-134
Identity = 184/202 (91.09%), Postives = 186/202 (92.08%), Query Frame = -2 
Query:  256 RWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 861
            +WNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:  978 KWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 1164

HSP 5 Score: 109.768 bits (273), Expect = 3.093e-134
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct: 1161 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 1231

HSP 6 Score: 68.9366 bits (167), Expect = 3.093e-134
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = -1 
Query:  911 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKY 1003
            VKSSHFRIQLIHKSNFKEWIFEEHQLKKNK+
Sbjct:  949 VKSSHFRIQLIHKSNFKEWIFEEHQLKKNKW 979

HSP 7 Score: 373.244 bits (957), Expect = 1.082e-129
Identity = 200/217 (92.17%), Postives = 201/217 (92.63%), Query Frame = -2 
Query:  256 MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGXXXXXXXXXXXXLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLELGKATRDK*IFDNCIVGLHIGFYMELRSDEDATILYFSSSTALDQSIKFRVRNNYIDI 906
            MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL               GLHIGFYMELRSDEDATILYFSSSTALD SIKFRVRNNYID+
Sbjct:    1 MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLNDLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESGKAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIKINLNDPQLEL---------------GLHIGFYMELRSDEDATILYFSSSTALDHSIKFRVRNNYIDL 202

HSP 8 Score: 109.768 bits (273), Expect = 1.082e-129
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = -1 
Query:    2 Y*NPSENNDNIINIAMRMLIEFRWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 217
            Y + S N +N+    M++L + +WYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK
Sbjct:  199 YIDLSINGNNLKFENMKIL-KSKWYKMDIFVNESATVLTVNCEQTYSSVNAEGLSKWKQMDLLTMICADEGK 269
The following BLAST results are available for this feature:
BLAST of SMED30029875 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30029875 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000077977.17.012e-13591.09SMESG000077977.1[more]
SMESG000077977.11.241e-13491.09SMESG000077977.1[more]
SMESG000077977.11.896e-13491.09SMESG000077977.1[more]
SMESG000077977.11.928e-13491.09SMESG000077977.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30029875 ID=SMED30029875|Name=SMED30029875|organism=Schmidtea mediterranea sexual|type=transcript|length=1009bp
CCTTGCCCTCATCTGCACAAATCATTGTCAATAAATCCATTTGTTTCCAT
TTTGATAATCCTTCCGCATTGACAGAAGAATATGTCTGCTCACAATTGAC
AGTCAATACTGTTGCACTTTCATTCACAAATATATCCATTTTGTACCACC
TAAATTCAATCAACATCCGCATTGCAATATTAATTATATTATCATTATTC
TCACTTGGATTTCAATATTTTCATATTTTCGAATTTCAAATTATTTCCAT
TAATCGATATCGATATAATTATTTCTCACTCGGAATTTTATCGATTGGTC
AAGTGCAGTTGATGAGGAAAAATAGAGAATTGTCGCGTCCTCATCACTCC
TCAATTCCATATAAAATCCGATATGCAATCCTACAATACAATTATCAAAT
ATTCATTTATCTCGAGTCGCTTTACCCAATTCCAATTGTGGATCATTCAA
ATTTATCTTTATCGGTTCCATTGATCCGGTGAGATCTTGATCTGGTTTCG
ATAGAATTTCTATAGTTTTCTCATCACAATTGATGTGCTTAACTGTCAAT
TCTTTCATTAGTTGATTCGATATTGCATTGGCTATTGAATTCGCTGTTCC
AGCTTTACCGGATTCTGTGAAACATTGATCGATTGTGCATGTCAAATGGC
TGACATCTGTCAAACTCGATAATGGAATCAATGATAATTTCGATATTGAT
CCCATGAAATATTCGTTAGAACTTTCATCAATCAAGAAATCTAATTTCCT
CAAATCATTCAATTTCATATTTTCATCAATTGGAATCGCAGATATTTCTC
CACAATTCATTCTTAGACTGATTTCTGCAAATGAAATCTGAATTTCTACT
TTGTTCCATCTGAAATGCAGATTTCGTGTTATCAATGCCATATTTGATAT
GATCATTTCAATACTTGTTCTTTTTCAATTGATGTTCTTCGAATATCCAT
TCTTTGAAATTTGATTTGTGTATCAATTGAATTCGGAAATGTGAGGATTT
CACAGATCG
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protein sequence of SMED30029875-orf-1

>SMED30029875-orf-1 ID=SMED30029875-orf-1|Name=SMED30029875-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=168bp
MIISNMALITRNLHFRWNKVEIQISFAEISLRMNCGEISAIPIDENMKLN
DLRKLDFLIDESSNEYFMGSISKLSLIPLSSLTDVSHLTCTIDQCFTESG
KAGTANSIANAISNQLMKELTVKHINCDEKTIEILSKPDQDLTGSMEPIK
INLNDPQLELGKATRDK*
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protein sequence of SMED30029875-orf-2

>SMED30029875-orf-2 ID=SMED30029875-orf-2|Name=SMED30029875-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=160bp
LPSSAQIIVNKSICFHFDNPSALTEEYVCSQLTVNTVALSFTNISILYHL
NSINIRIAILIILSLFSLGFQYFHIFEFQIISINRYRYNYFSLGILSIGQ
VQLMRKNRELSRPHHSSIPYKIRYAILQYNYQIFIYLESLYPIPIVDHSN
LSLSVPLIR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000428musculature system